MD13G1064000.v1.1

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011931
Physical Location & Seq
Reverse (-)
4412978 .. 4415584
2607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1064000.v1.1.491

Sequence Viewer

Length: 963 bp
ATGGAAGACGATGAAGAAGCTCCACCTCTCGCCATTGAAATCGACCAACCCCTGGGACCCTACTCTCAGCAGCAACCCAATTCATCTAGTCACAAAGACGACGACGTGTTGGTCTGTGTCACCGTCATCACCGGCTACCTCGGCGCCGGAAAATCCACCCTGGTTAATCATATATTGAAATCCCAACACGGGAAGAAAATTGCTGTGATACTAAACGATTTCGGTGAGGAAATTGGCGTGGAGAGAGCGATGATTAACCAAGGAGATGGCGGTGCGCTTGTCGAAGAGTGGGTGGAGTTAGCAAAAGGTATTTGTTGCACTGTTAAGCACAGTTTGGTGCAGGCATTGGAGCAACTTGTTCCCAAAAGAAAACTTGATCGTATGTTGCTGGAGACTACTGGTTGCGAAGACTGTGAGTTGGATGAAAATCTAGAATCATCTGTCAAGCTTGATTCAAGTATCACTGTCGTGGATGCCAAGAACCTCCGTTATCAGCTCAGTGAGCATCGAATCTCATCTTCATTTGCTGAAGCATATCTTCAGATTGCATTTGCATTTTACTCATATGATCTGTTCTATTCTAAGGGTTCTGCGGATTTTCTGGAGGAACTGGAGAAGGAAATACATAGTATTAACTCACTTGCTAGTATCATCCGTTCTGTCCGTGGTCAAGTTGACCTGTCCAATATATTGGACTGTCGGGCCTATGATGCCACGGTTCGTTTGTGGATTGAGGAGATTCTTTGGGAGAAGAAACATGACATGGATGTATACCGCTGCAAAGGGGTTCTGAGCGTTTATAATTCAAATCAATTACACACTTTGCAGGGAGTCAGCTTTTGTTCTTCTCCACATTCTAACCGGGTGATCCAGGAATTTGCCTATTTGCATTTCAGTGGGCTTGAACGAATCCGACGAAGAATCCCTTGCTTGATAGGGCTTGAGGTTAGATATCCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

321

Amino Acids

36.11

Weight (kDa)

5.14

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 40 - 185 1.9e-32 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26520
fragaria_vesca FvH4_4g30300
malus_domestica MD13G1064000.v1.1 MD16G1062700.v1.1
prunus_persica Prupe.1G284700_v2.0.a1
pyrus_communis pycom16g05480
rosa_chinensis RchiOBHm_Chr4g0438581
rosa_laevigata RLG00000006349
rosa_multiflora Rmu_co8071018.1_g000001 Rmu_sc0002327.1_g000002 Rmu_sc0020430.1_g000001
rosa_roxburghii Rroxscaffold_5G00379590
rosa_rugosa Rorug04G0311700
rosa_samantha Rh4AG362100 Rh4BG373500 Rh4CG387400 Rh4DG368200
rosa_wichuraiana Rw0G006680 Rw0G016630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 801
AasI GACNNNNNNGTC 1 cut(s) 110
AccB1I GGYRCC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 52
AccI GTMKAC 1 cut(s) 771
AciI CCGC 3 cut(s) 270, 593, 775
AclWI GGATC 1 cut(s) 862
AcsI RAATTY 1 cut(s) 875
AcuI CTGAAG 2 cut(s) 524, 549
AcyI GRCGYC 1 cut(s) 144
AfiI CCNNNNNNNGG 2 cut(s) 52, 189
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 5 cut(s) 38, 178, 456, 807, 905
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 3 cut(s) 51, 159, 870
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 4 cut(s) 20, 448, 496, 837
AluI AGCT 4 cut(s) 20, 448, 496, 837
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 1 cut(s) 862
AoxI GGCC 1 cut(s) 702
ApeKI GCWGC 2 cut(s) 70, 777
ApoI RAATTY 1 cut(s) 875
AspLEI GCGC 2 cut(s) 146, 277
AspS9I GGNCC 2 cut(s) 56, 702
AsuC2I CCSGG 1 cut(s) 863
AsuHPI GGTGA 4 cut(s) 112, 121, 236, 877
AvaII GGWCC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 143
BbsI GAAGAC 2 cut(s) 12, 414
BbvI GCAGC 2 cut(s) 82, 764
BccI CCATC 1 cut(s) 260
BciT130I CCWGG 3 cut(s) 53, 161, 872
BcnI CCSGG 1 cut(s) 863
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 3 cut(s) 87, 431, 645
BfoI RGCGCY 1 cut(s) 147
BisI GCNGC 2 cut(s) 71, 778
BlsI GCNGC 2 cut(s) 72, 779
Bme1390I CCNGG 4 cut(s) 53, 161, 863, 872
Bme18I GGWCC 1 cut(s) 56
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 2 cut(s) 56, 702
BmiI GGNNCC 3 cut(s) 57, 58, 145
BmrFI CCNGG 4 cut(s) 53, 161, 863, 872
BmsI GCATC 3 cut(s) 463, 514, 700
BpiI GAAGAC 2 cut(s) 12, 414
BpmI CTGGAG 3 cut(s) 410, 623, 632
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 863
BsaBI GATNNNNATC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 7 cut(s) 51, 52, 139, 159, 259, 664, 714
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 189
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 403, 615
Bse8I GATNNNNATC 1 cut(s) 426
BseBI CCWGG 3 cut(s) 53, 161, 872
BseDI CCNNGG 7 cut(s) 51, 52, 139, 159, 259, 664, 714
BseGI GGATG 4 cut(s) 427, 478, 651, 772
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 2 cut(s) 52, 189
BseMII CTCAG 3 cut(s) 80, 511, 782
BseNI ACTGG 2 cut(s) 403, 615
BseRI GAGGAG 1 cut(s) 749
BseXI GCAGC 2 cut(s) 82, 764
BsgI GTGCAG 1 cut(s) 359
BshFI GGCC 1 cut(s) 704
BshNI GGYRCC 1 cut(s) 143
BsiSI CCGG 3 cut(s) 132, 147, 862
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 52, 189
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 1 cut(s) 704
Bsp143I GATC 3 cut(s) 376, 568, 867
BspACI CCGC 3 cut(s) 270, 593, 775
BspANI GGCC 1 cut(s) 704
BspCNI CTCAG 3 cut(s) 79, 510, 783
BspLI GGNNCC 3 cut(s) 57, 58, 145
BspPI GGATC 1 cut(s) 862
BspT107I GGYRCC 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 2 cut(s) 403, 615
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 7 cut(s) 51, 52, 139, 159, 259, 664, 714
BssMI GATC 3 cut(s) 376, 568, 867
BssNAI GTATAC 1 cut(s) 772
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 259
Bst1107I GTATAC 1 cut(s) 772
Bst2UI CCWGG 3 cut(s) 53, 161, 872
Bst4CI ACNGT 7 cut(s) 124, 322, 332, 413, 466, 698, 718
Bst6I CTCTTC 1 cut(s) 279
BstACI GRCGYC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 4 cut(s) 66, 497, 582, 791
BstDSI CCRYGG 2 cut(s) 664, 714
BstF5I GGATG 4 cut(s) 427, 478, 651, 772
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 2 cut(s) 146, 277
BstKTI GATC 3 cut(s) 379, 571, 870
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 3 cut(s) 376, 568, 867
BstMWI GCNNNNNNNGC 3 cut(s) 141, 502, 710
BstNI CCWGG 3 cut(s) 53, 161, 872
BstSCI CCNGG 4 cut(s) 51, 159, 861, 870
BstV1I GCAGC 2 cut(s) 82, 764
BstV2I GAAGAC 2 cut(s) 12, 414
BstXI CCANNNNNNTGG 2 cut(s) 266, 691
BstZ17I GTATAC 1 cut(s) 772
BsuRI GGCC 1 cut(s) 704
BtgI CCRYGG 2 cut(s) 664, 714
BtgZI GCGATG 1 cut(s) 263
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 4 cut(s) 427, 478, 651, 772
BtsIMutI CAGTG 4 cut(s) 318, 462, 505, 901
Cac8I GCNNGC 1 cut(s) 342
CfoI GCGC 2 cut(s) 146, 277
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 56, 702
CviAII CATG 2 cut(s) 758, 763
CviJI RGCY 8 cut(s) 20, 135, 448, 496, 704, 837, 901, 940
CviKI_1 RGCY 8 cut(s) 20, 135, 448, 496, 704, 837, 901, 940
DdeI CTNAG 4 cut(s) 66, 497, 582, 791
DinI GGCGCC 1 cut(s) 145
DpnI GATC 3 cut(s) 378, 570, 869
DpnII GATC 3 cut(s) 376, 568, 867
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 259
Eco32I GATATC 1 cut(s) 953
Eco47I GGWCC 1 cut(s) 56
Eco57I CTGAAG 2 cut(s) 524, 549
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 3 cut(s) 51, 159, 870
EcoRV GATATC 1 cut(s) 953
EcoT14I CCWWGG 1 cut(s) 259
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 2 cut(s) 761, 766
FalI AAGNNNNNCTT 4 cut(s) 522, 554, 910, 942
FaqI GGGAC 1 cut(s) 69
FatI CATG 2 cut(s) 757, 762
FauNDI CATATG 1 cut(s) 565
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 2 cut(s) 71, 778
FokI GGATG 4 cut(s) 434, 485, 638, 779
Fsp4HI GCNGC 2 cut(s) 71, 778
FspBI CTAG 3 cut(s) 87, 431, 645
GlaI GCGC 2 cut(s) 145, 276
GluI GCNGC 2 cut(s) 71, 778
GsuI CTGGAG 3 cut(s) 410, 623, 632
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 1 cut(s) 704
HapII CCGG 3 cut(s) 132, 147, 862
HhaI GCGC 2 cut(s) 146, 277
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 2 cut(s) 761, 766
Hin6I GCGC 2 cut(s) 144, 275
HinP1I GCGC 2 cut(s) 144, 275
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 7 cut(s) 434, 452, 510, 739, 831, 909, 921
HpaII CCGG 3 cut(s) 132, 147, 862
HphI GGTGA 4 cut(s) 112, 121, 236, 877
Hpy166II GTNNAC 2 cut(s) 676, 772
Hpy188I TCNGA 3 cut(s) 543, 792, 914
Hpy188III TCNNGA 2 cut(s) 431, 602
Hpy8I GTNNAC 2 cut(s) 676, 772
Hpy99I CGWCG 3 cut(s) 104, 107, 918
HpyAV CCTTC 1 cut(s) 610
HpyCH4III ACNGT 7 cut(s) 124, 322, 332, 413, 466, 698, 718
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 7 cut(s) 318, 340, 548, 554, 780, 826, 889
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 502, 710
HpyF3I CTNAG 4 cut(s) 66, 497, 582, 791
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 2 cut(s) 761, 766
HspAI GCGC 2 cut(s) 144, 275
KasI GGCGCC 1 cut(s) 143
KflI GGGWCCC 1 cut(s) 56
Kzo9I GATC 3 cut(s) 376, 568, 867
LmnI GCTCC 2 cut(s) 25, 349
Lsp1109I GCAGC 2 cut(s) 82, 764
LweI GCATC 3 cut(s) 463, 514, 700
MaeI CTAG 3 cut(s) 87, 431, 645
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 89, 118
MalI GATC 3 cut(s) 378, 570, 869
MboI GATC 3 cut(s) 376, 568, 867
MluCI AATT 6 cut(s) 79, 198, 231, 802, 812, 875
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 1 cut(s) 840
MmeI TCCRAC 2 cut(s) 399, 937
MnlI CCTC 7 cut(s) 36, 149, 220, 494, 598, 727, 937
MseI TTAA 4 cut(s) 165, 255, 324, 633
MslI CAYNNNNRTG 2 cut(s) 467, 894
MspA1I CMGCKG 1 cut(s) 777
MspI CCGG 3 cut(s) 132, 147, 862
MspR9I CCNGG 4 cut(s) 53, 161, 863, 872
MvaI CCWGG 3 cut(s) 53, 161, 872
MwoI GCNNNNNNNGC 3 cut(s) 141, 502, 710
NarI GGCGCC 1 cut(s) 144
NciI CCSGG 1 cut(s) 863
NdeI CATATG 1 cut(s) 565
NdeII GATC 3 cut(s) 376, 568, 867
NlaIII CATG 2 cut(s) 761, 766
NlaIV GGNNCC 3 cut(s) 57, 58, 145
NmeAIII GCCGAG 1 cut(s) 120
NmuCI GTSAC 2 cut(s) 89, 118
OliI CACNNNNGTG 1 cut(s) 467
PasI CCCWGGG 1 cut(s) 52
PcsI WCGNNNNNNNCGW 1 cut(s) 913
PfeI GAWTC 6 cut(s) 434, 452, 510, 739, 909, 921
PflMI CCANNNNNTGG 1 cut(s) 52
PfoI TCCNGGA 1 cut(s) 870
PkrI GCNGC 2 cut(s) 72, 779
PleI GAGTC 1 cut(s) 839
PluTI GGCGCC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 839
PpuMI RGGWCCY 1 cut(s) 56
PsiI TTATAA 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 3 cut(s) 51, 159, 870
PspGI CCWGG 3 cut(s) 51, 159, 870
PspN4I GGNNCC 3 cut(s) 57, 58, 145
PspPI GGNCC 2 cut(s) 56, 702
PspPPI RGGWCCY 1 cut(s) 56
PsrI GAACNNNNNNTAC 2 cut(s) 640, 672
RseI CAYNNNNRTG 2 cut(s) 467, 894
SaqAI TTAA 4 cut(s) 165, 255, 324, 633
SatI GCNGC 2 cut(s) 71, 778
Sau3AI GATC 3 cut(s) 376, 568, 867
Sau96I GGNCC 2 cut(s) 56, 702
SchI GAGTC 1 cut(s) 840
ScrFI CCNGG 4 cut(s) 53, 161, 863, 872
SfaNI GCATC 3 cut(s) 463, 514, 700
SfoI GGCGCC 1 cut(s) 145
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 2 cut(s) 467, 894
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
Sse9I AATT 6 cut(s) 79, 198, 231, 802, 812, 875
SsiI CCGC 3 cut(s) 270, 593, 775
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 3 cut(s) 87, 431, 645
StyD4I CCNGG 4 cut(s) 51, 159, 861, 870
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 7 cut(s) 124, 322, 332, 413, 466, 698, 718
TaiI ACGT 1 cut(s) 108
TaqI TCGA 3 cut(s) 42, 282, 508
TasI AATT 6 cut(s) 79, 198, 231, 802, 812, 875
TfiI GAWTC 6 cut(s) 434, 452, 510, 739, 909, 921
Tru1I TTAA 4 cut(s) 165, 255, 324, 633
Tru9I TTAA 4 cut(s) 165, 255, 324, 633
TscAI CASTG 4 cut(s) 325, 469, 505, 901
TseFI GTSAC 2 cut(s) 89, 118
TseI GCWGC 2 cut(s) 70, 777
Tsp45I GTSAC 2 cut(s) 89, 118
TspDTI ATGAA 4 cut(s) 27, 72, 438, 510
TspGWI ACGGA 3 cut(s) 476, 644, 653
TspRI CASTG 4 cut(s) 325, 469, 505, 901
Van91I CCANNNNNTGG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 1 cut(s) 875
XbaI TCTAGA 1 cut(s) 430
XmiI GTMKAC 1 cut(s) 771
XspI CTAG 3 cut(s) 87, 431, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.