Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011931

Physical Location & Seq

Genomic Coordinates Reverse (-)

4452319 .. 4455138

Gene Structure 2820 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1062700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGGAAGACGATGAAGAAGCTCCACCTCTCGCCGTTGAAATCGATCAAGCGACGGAACCCTACTCTCAGCAGCAGCCCAATTCATCTAGTCGCATAGACGACGACGTTTCGGTCGGTGTCACCGTCATCACCGGCTATCTTGGCGCCGGAAAATCCACTCTGGTTAATCATATATTGAATTCCCAACACGGGAAGAGAATTGCTGTGATATTAAACGAGTTTGGTGAGGAAATTGGCGTGGAGAGAGCGATGATTAACGAAGGAGATGGCGGTGCGCTTGTCGAAGAGTGGGTGGAGTTAGCAAATGGGTGTATTTGTTGCACTGTTAAGCACAGTTTGGTGCAGGCATTGGAGCAACTTGTTCAGAGAAAAGAAAGACTGGATCATATATTGCTGGAGACTACTGGGTTAGCGAACCCAGCTCCGCTTGCTTCGGTTCTGTGGTTGGATGATAATCTAGAATCGTCTGTCAAACTTGATTCAATTATCACTGTTGTAGATGCCAAGAACCTCCGTTTTCAGCTCAGTGAAAATCGAAGTTCATCTTCATTTCCTGAAGCATATCTTCAAATTGCGTTTGCGGATGTTATAATTCTTAACAAGGTTGATTTGGTTTCTCCAGAGGATTCTGCCAATTTTCTGGAGGAACTGGAGAAGGAAATACATAGTATTAACTCTCTTGCTAGTATCATTCGTTCTGTTCGTGGTCAAGTTGACTTGTCCAATATATTGGACTGTCGAGCCTATGATGCCACGCATGCTACCCATTTAGAATCATTATTGGAAGAAAGTCGTGCTCTATCTACAAGTGATCTTCATGATAGTGGTGTGCGAACTTTATGCATTTCCGAGCCACAGCAGGTTGATCTTGAAAAGGTTCGTTTGTGGATTGAGGAGATTCTTTGGGAGAAGAAAGATGGCATGGATGTATACCGCTGCAAAGGGGTTTTGAGCATTCATAATTCAAATCAATTACATACTTTGCAGGGAGTGAAGGAGATATATGAGATTGTGCCCACTCGTGATTGGAAAACGGAAGAAACTCAGACGAACAAGATAGTGTTTATAGGTCATAATCTAAATGAGAATGTTCTCGCCGAGTCTTTTAGGTCGTGCGTAGTTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CobW_C KTI12 cobW

Protein Analysis

376

Amino Acids

41.73

Weight (kDa)

4.62

Isoelectric Point (pI)

52.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
cobW	PF02492	40 - 231	3.4e-57	CobW/HypB/UreG, nucleotide-binding domain
CobW_C	PF07683	278 - 373	4.5e-18	Cobalamin synthesis protein cobW C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011931)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26520
fragaria_vesca	FvH4_4g30300
malus_domestica	MD13G1064000.v1.1 MD16G1062700.v1.1
prunus_persica	Prupe.1G284700_v2.0.a1
pyrus_communis	pycom16g05480
rosa_chinensis	RchiOBHm_Chr4g0438581
rosa_laevigata	RLG00000006349
rosa_multiflora	Rmu_co8071018.1_g000001 Rmu_sc0002327.1_g000002 Rmu_sc0020430.1_g000001
rosa_roxburghii	Rroxscaffold_5G00379590
rosa_rugosa	Rorug04G0311700
rosa_samantha	Rh4AG362100 Rh4BG373500 Rh4CG387400 Rh4DG368200
rosa_wichuraiana	Rw0G006680 Rw0G016630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	590
AasI	GACNNNNNNGTC	1 cut(s)	110
Acc36I	ACCTGC	1 cut(s)	850
AccB1I	GGYRCC	1 cut(s)	143
AccI	GTMKAC	1 cut(s)	930
AciI	CCGC	4 cut(s)	270, 425, 581, 934
AclWI	GGATC	1 cut(s)	390
AcsI	RAATTY	1 cut(s)	178
AcuI	CTGAAG	1 cut(s)	576
AcyI	GRCGYC	1 cut(s)	144
AfiI	CCNNNNNNNGG	1 cut(s)	189
AgsI	TTSAA	6 cut(s)	38, 178, 483, 569, 872, 966
AluBI	AGCT	3 cut(s)	20, 422, 523
AluI	AGCT	3 cut(s)	20, 422, 523
Alw21I	GWGCWC	1 cut(s)	799
Alw26I	GTCTC	1 cut(s)	392
AlwI	GGATC	1 cut(s)	390
ApeKI	GCWGC	3 cut(s)	70, 73, 936
ApoI	RAATTY	1 cut(s)	178
Asp700I	GAANNNNTTC	1 cut(s)	876
AspLEI	GCGC	2 cut(s)	146, 277
AsuHPI	GGTGA	3 cut(s)	112, 121, 236
BaeGI	GKGCMC	1 cut(s)	1017
BanI	GGYRCC	1 cut(s)	143
BauI	CACGAG	1 cut(s)	1020
BbsI	GAAGAC	1 cut(s)	12
Bbv12I	GWGCWC	1 cut(s)	799
BbvI	GCAGC	3 cut(s)	82, 85, 923
BccI	CCATC	2 cut(s)	260, 911
BceAI	ACGGC	1 cut(s)	17
BcgI	CGANNNNNNTGC	2 cut(s)	822, 856
BcoDI	GTCTC	1 cut(s)	392
BfaI	CTAG	3 cut(s)	87, 458, 684
BfoI	RGCGCY	1 cut(s)	147
BfuAI	ACCTGC	1 cut(s)	850
BisI	GCNGC	3 cut(s)	71, 74, 937
BlsI	GCNGC	3 cut(s)	72, 75, 938
BmiI	GGNNCC	2 cut(s)	57, 145
BmrI	ACTGGG	1 cut(s)	414
BmsI	GCATC	2 cut(s)	490, 739
BmuI	ACTGGG	1 cut(s)	414
BpiI	GAAGAC	1 cut(s)	12
BpmI	CTGGAG	4 cut(s)	416, 603, 662, 671
Bsa29I	ATCGAT	1 cut(s)	42
BsaBI	GATNNNNATC	1 cut(s)	453
BsaHI	GRCGYC	1 cut(s)	144
Bsc4I	CCNNNNNNNGG	1 cut(s)	189
Bse118I	RCCGGY	1 cut(s)	131
Bse1I	ACTGG	3 cut(s)	384, 409, 654
Bse8I	GATNNNNATC	1 cut(s)	453
BseCI	ATCGAT	1 cut(s)	42
BseGI	GGATG	3 cut(s)	454, 589, 931
BseJI	GATNNNNATC	1 cut(s)	453
BseLI	CCNNNNNNNGG	1 cut(s)	189
BseMII	CTCAG	3 cut(s)	80, 538, 1058
BseNI	ACTGG	3 cut(s)	384, 409, 654
BseRI	GAGGAG	1 cut(s)	908
BseSI	GKGCMC	1 cut(s)	1017
BseXI	GCAGC	3 cut(s)	82, 85, 923
BseYI	CCCAGC	1 cut(s)	418
BsgI	GTGCAG	1 cut(s)	362
Bsh1285I	CGRYCG	1 cut(s)	114
BshNI	GGYRCC	1 cut(s)	143
BshVI	ATCGAT	1 cut(s)	42
BsiEI	CGRYCG	1 cut(s)	114
BsiHKAI	GWGCWC	1 cut(s)	799
BsiSI	CCGG	2 cut(s)	132, 147
BslI	CCNNNNNNNGG	1 cut(s)	189
BsmAI	GTCTC	1 cut(s)	392
BsmI	GAATGC	1 cut(s)	954
Bsp1286I	GDGCHC	2 cut(s)	799, 1017
Bsp143I	GATC	4 cut(s)	43, 382, 811, 865
BspACI	CCGC	4 cut(s)	270, 425, 581, 934
BspCNI	CTCAG	3 cut(s)	79, 537, 1057
BspDI	ATCGAT	1 cut(s)	42
BspHI	TCATGA	1 cut(s)	817
BspLI	GGNNCC	2 cut(s)	57, 145
BspMI	ACCTGC	1 cut(s)	850
BspPI	GGATC	1 cut(s)	390
BspT107I	GGYRCC	1 cut(s)	143
BsrFI	RCCGGY	1 cut(s)	131
BsrI	ACTGG	3 cut(s)	384, 409, 654
BssAI	RCCGGY	1 cut(s)	131
BssMI	GATC	4 cut(s)	43, 382, 811, 865
BssNAI	GTATAC	1 cut(s)	931
BssNI	GRCGYC	1 cut(s)	144
BssSI	CACGAG	1 cut(s)	1020
Bst1107I	GTATAC	1 cut(s)	931
Bst2BI	CACGAG	1 cut(s)	1020
Bst4CI	ACNGT	5 cut(s)	124, 325, 335, 493, 737
Bst6I	CTCTTC	2 cut(s)	188, 279
BstACI	GRCGYC	1 cut(s)	144
BstC8I	GCNNGC	3 cut(s)	345, 429, 759
BstDEI	CTNAG	3 cut(s)	66, 524, 1044
BstF5I	GGATG	3 cut(s)	454, 589, 931
BstH2I	RGCGCY	1 cut(s)	147
BstHHI	GCGC	2 cut(s)	146, 277
BstKTI	GATC	4 cut(s)	46, 385, 814, 868
BstMAI	GTCTC	1 cut(s)	392
BstMBI	GATC	4 cut(s)	43, 382, 811, 865
BstMCI	CGRYCG	1 cut(s)	114
BstMWI	GCNNNNNNNGC	5 cut(s)	141, 419, 428, 749, 758
BstNSI	RCATGY	1 cut(s)	761
BstSLI	GKGCMC	1 cut(s)	1017
BstV1I	GCAGC	3 cut(s)	82, 85, 923
BstV2I	GAAGAC	1 cut(s)	12
BstXI	CCANNNNNNTGG	2 cut(s)	640, 730
BstZ17I	GTATAC	1 cut(s)	931
Bsu15I	ATCGAT	1 cut(s)	42
BsuTUI	ATCGAT	1 cut(s)	42
BtgZI	GCGATG	1 cut(s)	263
BtsCI	GGATG	3 cut(s)	454, 589, 931
BtsIMutI	CAGTG	3 cut(s)	321, 489, 532
BveI	ACCTGC	1 cut(s)	850
Cac8I	GCNNGC	3 cut(s)	345, 429, 759
CciI	TCATGA	1 cut(s)	817
CfoI	GCGC	2 cut(s)	146, 277
Cfr10I	RCCGGY	1 cut(s)	131
ClaI	ATCGAT	1 cut(s)	42
CviAII	CATG	3 cut(s)	758, 818, 922
CviJI	RGCY	7 cut(s)	20, 76, 135, 422, 523, 743, 853
CviKI_1	RGCY	7 cut(s)	20, 76, 135, 422, 523, 743, 853
DdeI	CTNAG	3 cut(s)	66, 524, 1044
DinI	GGCGCC	1 cut(s)	145
DpnI	GATC	4 cut(s)	45, 384, 813, 867
DpnII	GATC	4 cut(s)	43, 382, 811, 865
DrdI	GACNNNNNNGTC	1 cut(s)	110
DseDI	GACNNNNNNGTC	1 cut(s)	110
Eam1104I	CTCTTC	2 cut(s)	188, 279
EarI	CTCTTC	2 cut(s)	188, 279
Eco57I	CTGAAG	1 cut(s)	576
EcoRI	GAATTC	1 cut(s)	178
EcoT22I	ATGCAT	1 cut(s)	845
EgeI	GGCGCC	1 cut(s)	145
EheI	GGCGCC	1 cut(s)	145
FaeI	CATG	3 cut(s)	761, 821, 925
FalI	AAGNNNNNCTT	4 cut(s)	529, 561, 549, 581
FatI	CATG	3 cut(s)	757, 817, 921
FblI	GTMKAC	1 cut(s)	930
Fnu4HI	GCNGC	3 cut(s)	71, 74, 937
FokI	GGATG	3 cut(s)	461, 596, 938
Fsp4HI	GCNGC	3 cut(s)	71, 74, 937
FspBI	CTAG	3 cut(s)	87, 458, 684
GlaI	GCGC	2 cut(s)	145, 276
GluI	GCNGC	3 cut(s)	71, 74, 937
GsaI	CCCAGC	1 cut(s)	422
GsuI	CTGGAG	4 cut(s)	416, 603, 662, 671
HaeII	RGCGCY	1 cut(s)	147
HapII	CCGG	2 cut(s)	132, 147
HhaI	GCGC	2 cut(s)	146, 277
Hin1I	GRCGYC	1 cut(s)	144
Hin1II	CATG	3 cut(s)	761, 821, 925
Hin6I	GCGC	2 cut(s)	144, 275
HinP1I	GCGC	2 cut(s)	144, 275
HincII	GTYRAC	1 cut(s)	715
HindII	GTYRAC	1 cut(s)	715
HinfI	GANTC	6 cut(s)	461, 479, 626, 773, 898, 1100
HpaII	CCGG	2 cut(s)	132, 147
HphI	GGTGA	3 cut(s)	112, 121, 236
Hpy166II	GTNNAC	2 cut(s)	715, 931
Hpy188I	TCNGA	3 cut(s)	366, 850, 1047
Hpy188III	TCNNGA	7 cut(s)	458, 554, 620, 641, 818, 869, 1022
Hpy8I	GTNNAC	2 cut(s)	715, 931
Hpy99I	CGWCG	3 cut(s)	55, 104, 107
HpyAV	CCTTC	3 cut(s)	254, 649, 988
HpyCH4III	ACNGT	5 cut(s)	124, 325, 335, 493, 737
HpyCH4IV	ACGT	1 cut(s)	105
HpyCH4V	TGCA	5 cut(s)	321, 343, 843, 939, 985
HpyF10VI	GCNNNNNNNGC	5 cut(s)	141, 419, 428, 749, 758
HpyF3I	CTNAG	3 cut(s)	66, 524, 1044
HpySE526I	ACGT	1 cut(s)	105
Hsp92I	GRCGYC	1 cut(s)	144
Hsp92II	CATG	3 cut(s)	761, 821, 925
HspAI	GCGC	2 cut(s)	144, 275
KasI	GGCGCC	1 cut(s)	143
Kzo9I	GATC	4 cut(s)	43, 382, 811, 865
LmnI	GCTCC	3 cut(s)	25, 352, 427
Lsp1109I	GCAGC	3 cut(s)	82, 85, 923
LweI	GCATC	2 cut(s)	490, 739
MaeI	CTAG	3 cut(s)	87, 458, 684
MaeII	ACGT	1 cut(s)	105
MaeIII	GTNAC	1 cut(s)	118
MalI	GATC	4 cut(s)	45, 384, 813, 867
MboI	GATC	4 cut(s)	43, 382, 811, 865
MhlI	GDGCHC	2 cut(s)	799, 1017
Mly113I	GGCGCC	1 cut(s)	144
MlyI	GAGTC	1 cut(s)	1109
MmeI	TCCRAC	1 cut(s)	426
MnlI	CCTC	6 cut(s)	36, 220, 521, 616, 637, 886
Mph1103I	ATGCAT	1 cut(s)	845
MroXI	GAANNNNTTC	1 cut(s)	876
MseI	TTAA	6 cut(s)	165, 212, 255, 327, 597, 672
MslI	CAYNNNNRTG	1 cut(s)	822
MspA1I	CMGCKG	1 cut(s)	936
MspI	CCGG	2 cut(s)	132, 147
Mva1269I	GAATGC	1 cut(s)	954
MwoI	GCNNNNNNNGC	5 cut(s)	141, 419, 428, 749, 758
NarI	GGCGCC	1 cut(s)	144
NdeII	GATC	4 cut(s)	43, 382, 811, 865
NlaIII	CATG	3 cut(s)	761, 821, 925
NlaIV	GGNNCC	2 cut(s)	57, 145
NmeAIII	GCCGAG	1 cut(s)	1123
NmuCI	GTSAC	1 cut(s)	118
NsiI	ATGCAT	1 cut(s)	845
NspI	RCATGY	1 cut(s)	761
PaeI	GCATGC	1 cut(s)	761
PagI	TCATGA	1 cut(s)	817
PcsI	WCGNNNNNNNCGW	2 cut(s)	120, 700
PctI	GAATGC	1 cut(s)	954
PdmI	GAANNNNTTC	1 cut(s)	876
PfeI	GAWTC	5 cut(s)	461, 479, 626, 773, 898
PkrI	GCNGC	3 cut(s)	72, 75, 938
PleI	GAGTC	1 cut(s)	1108
PluTI	GGCGCC	1 cut(s)	147
PpsI	GAGTC	1 cut(s)	1108
PsiI	TTATAA	1 cut(s)	590
PspFI	CCCAGC	1 cut(s)	418
PspN4I	GGNNCC	2 cut(s)	57, 145
PsrI	GAACNNNNNNTAC	2 cut(s)	679, 711
RseI	CAYNNNNRTG	1 cut(s)	822
SaqAI	TTAA	6 cut(s)	165, 212, 255, 327, 597, 672
SatI	GCNGC	3 cut(s)	71, 74, 937
Sau3AI	GATC	4 cut(s)	43, 382, 811, 865
SchI	GAGTC	1 cut(s)	1109
SduI	GDGCHC	2 cut(s)	799, 1017
SfaNI	GCATC	2 cut(s)	490, 739
SfoI	GGCGCC	1 cut(s)	145
SmiMI	CAYNNNNRTG	1 cut(s)	822
SphI	GCATGC	1 cut(s)	761
SsiI	CCGC	4 cut(s)	270, 425, 581, 934
SspDI	GGCGCC	1 cut(s)	143
SspMI	CTAG	3 cut(s)	87, 458, 684
TaaI	ACNGT	5 cut(s)	124, 325, 335, 493, 737
TaiI	ACGT	1 cut(s)	108
TaqI	TCGA	4 cut(s)	42, 282, 535, 739
TaqII	GACCGA	1 cut(s)	100
TfiI	GAWTC	5 cut(s)	461, 479, 626, 773, 898
Tru1I	TTAA	6 cut(s)	165, 212, 255, 327, 597, 672
Tru9I	TTAA	6 cut(s)	165, 212, 255, 327, 597, 672
TscAI	CASTG	3 cut(s)	328, 496, 532
TseFI	GTSAC	1 cut(s)	118
TseI	GCWGC	3 cut(s)	70, 73, 936
Tsp45I	GTSAC	1 cut(s)	118
TspDTI	ATGAA	6 cut(s)	27, 72, 531, 537, 806, 947
TspGWI	ACGGA	3 cut(s)	68, 503, 1049
TspRI	CASTG	3 cut(s)	328, 496, 532
XapI	RAATTY	1 cut(s)	178
XbaI	TCTAGA	1 cut(s)	457
XceI	RCATGY	1 cut(s)	761
XmiI	GTMKAC	1 cut(s)	930
XmnI	GAANNNNTTC	1 cut(s)	876
XspI	CTAG	3 cut(s)	87, 458, 684
Zsp2I	ATGCAT	1 cut(s)	845

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.