AT1G27050

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015289
Physical Location & Seq
Forward (+)
9393653 .. 9394716
1064 bp
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UTR
Exon/CDS
Intron
AT1G27050.1

Sequence Viewer

Length: 618 bp
ATGAGCCGCAAGAGAGATAAACCCTACACAAACCGTCACACACCGGCGCGAATTTCGAAACGCCGGCGACCATGGGCGCCTTCGTCATCGGAGCACGATGAGATTATCGACAAACCGATCACCAAACCTCCGCCGCCACCGGCGTTGGTAGTTATGGGACTTCCCGCCAACTGTTCAGTTCTGGAGCTAAAATCTCGATTCGAGATCTACGGTTCAATCTCCCGAATCCGAATCCATAAAGACGGAATTGGCTCCGTTTCGTATCGAACCGCCGAGTCAGCAGAAGCCGCCATTGCTGGTAGTCACGAGCCTTCTTTCGGTATCTCCATCGATTCCAAAAAGTTGGAGGTGGTTTGGGCGACGGATCCATTGGTGAAGTGGAAGGAAGGTGTGACGGCGGGAGAAGGAAAGGAGAGAACGTCGTCGTTTTCGTCGAAGCTTTTACGACCTGTGATGCCTTTGAGAAAACATGGGAGAAGCAGTAGGTTAGCGTCAGCTATTGTTAATCCGAGAAGTGATAATACTAAAGGAATTAGTGGAGATGGAGGGATATCATCTCCGGCGACGACAAGTGAAGTTAAACAGAGAAATATAGTAACCTACGACGATATCGTTTAA

Protein Analysis

205

Amino Acids

22.45

Weight (kDa)

9.99

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 49 - 99 4.9e-08 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015289)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27050
fragaria_vesca FvH4_4g32080
malus_domestica MD13G1079700.v1.1 MD13G1172800.v1.1
prunus_persica Prupe.1G267100_v2.0.a1
pyrus_communis pycom03g02960
rosa_chinensis RchiOBHm_Chr4g0440771
rosa_laevigata RLG00000006155
rosa_roxburghii Rroxscaffold_5G00381530
rosa_rugosa Rorug04G0327800
rosa_samantha Rh4AG379600 Rh4BG392500 Rh4CG407300 Rh4DG386300
rosa_wichuraiana Rw4G032650 Rw4G032680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AccII CGCG 1 cut(s) 49
AciI CCGC 7 cut(s) 7, 131, 134, 165, 270, 288, 398
AclWI GGATC 2 cut(s) 359, 372
AcsI RAATTY 1 cut(s) 51
AcyI GRCGYC 1 cut(s) 77
AfiI CCNNNNNNNGG 1 cut(s) 317
AgsI TTSAA 1 cut(s) 216
AluBI AGCT 3 cut(s) 187, 439, 497
AluI AGCT 3 cut(s) 187, 439, 497
Alw21I GWGCWC 1 cut(s) 96
AlwI GGATC 2 cut(s) 359, 372
ApoI RAATTY 1 cut(s) 51
AspLEI GCGC 2 cut(s) 49, 79
AsuHPI GGTGA 2 cut(s) 112, 385
AsuII TTCGAA 1 cut(s) 56
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 76
BarI GAAGNNNNNNTAC 2 cut(s) 505, 537
BauI CACGAG 1 cut(s) 305
Bbv12I GWGCWC 1 cut(s) 96
BccI CCATC 2 cut(s) 335, 536
BceAI ACGGC 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 80
BglII AGATCT 1 cut(s) 204
BisI GCNGC 3 cut(s) 7, 134, 288
BlsI GCNGC 3 cut(s) 8, 135, 289
BmiI GGNNCC 3 cut(s) 78, 253, 366
BmsI GCATC 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 203
Bpu14I TTCGAA 1 cut(s) 56
Bsa29I ATCGAT 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 71
BsaXI ACNNNNNCTCC 6 cut(s) 112, 142, 404, 434, 466, 496
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse118I RCCGGY 3 cut(s) 43, 63, 139
Bse3DI GCAATG 1 cut(s) 291
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 71
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMI GCAATG 1 cut(s) 291
Bsh1236I CGCG 1 cut(s) 49
BshNI GGYRCC 1 cut(s) 76
BshVI ATCGAT 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 4 cut(s) 44, 64, 140, 560
BslFI GGGAC 1 cut(s) 171
BslI CCNNNNNNNGG 1 cut(s) 317
BsmFI GGGAC 1 cut(s) 171
Bsp119I TTCGAA 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 3 cut(s) 117, 204, 364
Bsp19I CCATGG 1 cut(s) 71
BspACI CCGC 7 cut(s) 7, 131, 134, 165, 270, 288, 398
BspDI ATCGAT 1 cut(s) 330
BspFNI CGCG 1 cut(s) 49
BspLI GGNNCC 3 cut(s) 78, 253, 366
BspPI GGATC 2 cut(s) 359, 372
BspT104I TTCGAA 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 3 cut(s) 43, 63, 139
BssAI RCCGGY 3 cut(s) 43, 63, 139
BssECI CCNNGG 1 cut(s) 71
BssMI GATC 3 cut(s) 117, 204, 364
BssNI GRCGYC 1 cut(s) 77
BssSI CACGAG 1 cut(s) 305
BssT1I CCWWGG 1 cut(s) 71
Bst2BI CACGAG 1 cut(s) 305
Bst4CI ACNGT 3 cut(s) 35, 173, 212
BstACI GRCGYC 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 65
BstDSI CCRYGG 1 cut(s) 71
BstFNI CGCG 1 cut(s) 49
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 2 cut(s) 49, 79
BstKTI GATC 3 cut(s) 120, 207, 367
BstMBI GATC 3 cut(s) 117, 204, 364
BstMWI GCNNNNNNNGC 3 cut(s) 278, 287, 293
BstUI CGCG 1 cut(s) 49
BstX2I RGATCY 2 cut(s) 204, 364
BstXI CCANNNNNNTGG 1 cut(s) 343
BstYI RGATCY 2 cut(s) 204, 364
Bsu15I ATCGAT 1 cut(s) 330
BsuTUI ATCGAT 1 cut(s) 330
BtgI CCRYGG 1 cut(s) 71
Cac8I GCNNGC 1 cut(s) 65
CfoI GCGC 2 cut(s) 49, 79
Cfr10I RCCGGY 3 cut(s) 43, 63, 139
ClaI ATCGAT 1 cut(s) 330
CseI GACGC 1 cut(s) 480
CspCI CAANNNNNGTGG 2 cut(s) 126, 161
CviAII CATG 2 cut(s) 72, 470
CviJI RGCY 7 cut(s) 6, 187, 252, 287, 310, 439, 497
CviKI_1 RGCY 7 cut(s) 6, 187, 252, 287, 310, 439, 497
DinI GGCGCC 1 cut(s) 78
DpnI GATC 3 cut(s) 119, 206, 366
DpnII GATC 3 cut(s) 117, 204, 364
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 71
Eco32I GATATC 2 cut(s) 552, 610
EcoRV GATATC 2 cut(s) 552, 610
EcoT14I CCWWGG 1 cut(s) 71
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 2 cut(s) 75, 473
FaiI YATR 5 cut(s) 73, 155, 237, 471, 593
FaqI GGGAC 1 cut(s) 171
FatI CATG 2 cut(s) 71, 469
FauI CCCGC 2 cut(s) 172, 391
Fnu4HI GCNGC 3 cut(s) 7, 134, 288
Fsp4HI GCNGC 3 cut(s) 7, 134, 288
GlaI GCGC 2 cut(s) 48, 78
GluI GCNGC 3 cut(s) 7, 134, 288
GsuI CTGGAG 1 cut(s) 203
HaeII RGCGCY 1 cut(s) 80
HapII CCGG 4 cut(s) 44, 64, 140, 560
HgaI GACGC 1 cut(s) 480
HhaI GCGC 2 cut(s) 49, 79
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 2 cut(s) 75, 473
Hin6I GCGC 2 cut(s) 47, 77
HinP1I GCGC 2 cut(s) 47, 77
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 5 cut(s) 198, 225, 231, 275, 332
HpaII CCGG 4 cut(s) 44, 64, 140, 560
HphI GGTGA 2 cut(s) 112, 385
Hpy188I TCNGA 3 cut(s) 91, 230, 510
Hpy188III TCNNGA 5 cut(s) 182, 195, 202, 222, 305
Hpy99I CGWCG 6 cut(s) 364, 424, 427, 436, 568, 608
HpyAV CCTTC 5 cut(s) 90, 321, 376, 380, 398
HpyCH4III ACNGT 3 cut(s) 35, 173, 212
HpyCH4IV ACGT 1 cut(s) 419
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 287, 293
HpySE526I ACGT 1 cut(s) 419
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 2 cut(s) 75, 473
HspAI GCGC 2 cut(s) 47, 77
KasI GGCGCC 1 cut(s) 76
KroI GCCGGC 1 cut(s) 63
KroNI GCCGGC 1 cut(s) 65
Kzo9I GATC 3 cut(s) 117, 204, 364
LmnI GCTCC 3 cut(s) 91, 184, 257
LpnPI CCDG 7 cut(s) 57, 77, 153, 167, 282, 462, 573
LweI GCATC 1 cut(s) 444
MaeII ACGT 1 cut(s) 419
MaeIII GTNAC 4 cut(s) 35, 302, 391, 595
MalI GATC 3 cut(s) 119, 206, 366
MboI GATC 3 cut(s) 117, 204, 364
MflI RGATCY 2 cut(s) 204, 364
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 3 cut(s) 51, 246, 531
Mly113I GGCGCC 1 cut(s) 77
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 3 cut(s) 138, 340, 539
MreI CGCCGGCG 1 cut(s) 63
MroNI GCCGGC 1 cut(s) 63
MseI TTAA 3 cut(s) 504, 579, 616
MspI CCGG 4 cut(s) 44, 64, 140, 560
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 3 cut(s) 278, 287, 293
NaeI GCCGGC 1 cut(s) 65
NarI GGCGCC 1 cut(s) 77
NcoI CCATGG 1 cut(s) 71
NdeII GATC 3 cut(s) 117, 204, 364
NgoMIV GCCGGC 1 cut(s) 63
NlaIII CATG 2 cut(s) 75, 473
NlaIV GGNNCC 3 cut(s) 78, 253, 366
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 3 cut(s) 35, 302, 391
NspV TTCGAA 1 cut(s) 56
PcsI WCGNNNNNNNCGW 3 cut(s) 428, 431, 609
PdiI GCCGGC 1 cut(s) 65
PfeI GAWTC 4 cut(s) 198, 225, 231, 332
PkrI GCNGC 3 cut(s) 8, 135, 289
PleI GAGTC 1 cut(s) 283
PluTI GGCGCC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 283
PspN4I GGNNCC 3 cut(s) 78, 253, 366
PsuI RGATCY 2 cut(s) 204, 364
SaqAI TTAA 3 cut(s) 504, 579, 616
SatI GCNGC 3 cut(s) 7, 134, 288
Sau3AI GATC 3 cut(s) 117, 204, 364
SchI GAGTC 1 cut(s) 284
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 1 cut(s) 444
SfoI GGCGCC 1 cut(s) 78
SfuI TTCGAA 1 cut(s) 56
SgrAI CRCCGGYG 3 cut(s) 43, 63, 139
Sse9I AATT 3 cut(s) 51, 246, 531
SsiI CCGC 7 cut(s) 7, 131, 134, 165, 270, 288, 398
SspDI GGCGCC 1 cut(s) 76
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 3 cut(s) 35, 173, 212
TaiI ACGT 1 cut(s) 422
TaqI TCGA 7 cut(s) 56, 108, 196, 201, 265, 330, 434
TasI AATT 3 cut(s) 51, 246, 531
TauI GCSGC 3 cut(s) 9, 136, 290
TfiI GAWTC 4 cut(s) 198, 225, 231, 332
Tru1I TTAA 3 cut(s) 504, 579, 616
Tru9I TTAA 3 cut(s) 504, 579, 616
TseFI GTSAC 3 cut(s) 35, 302, 391
Tsp45I GTSAC 3 cut(s) 35, 302, 391
TspGWI ACGGA 3 cut(s) 244, 258, 377
XapI RAATTY 1 cut(s) 51
XcmI CCANNNNNNNNNTGG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.