pycom03g02960

sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015289
Physical Location & Seq
Forward (+)
2307360 .. 2308539
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g02960.3

Sequence Viewer

Length: 558 bp
ATGCATTTTTCCGTGCACGCTTCATGCCAAAAGAAGAACGAGCAAGCCACTTACTTAGAGGATTTGAAGGATCACATTGATGATTTTGTTAATGCATACTTGCTTCAAGACCACCGTGCAAAAGCCTCGACCGCTTCCTTTCTTTATCCCCCCTCCCTCCTCAACTCATATGGCAAACCCTCGTCCCTCTTCACCTCGTCTAAACACCATGAGCTAGAAGAGAGACAAACCCTACTTCTCCCGCCACGCCCCCTACTCCGGGTCGAAACACCGTCGTCCCCTGCCTCCACATCCGGAACCCAAACCCGAGCTCGACAAGCCCACTTACAAGCAACCTACCGTGCCACCGACTCCGCTCAAGCCGCCATTGCTGCCTCTCTCGACCCATCATTCGGTATCACCATCGAATCCAAAAAACTGCAGGTGCTTTGGCCGACGGACTCACTTGCCCAGTGGCGGAAGGGAGTTGGTGCTGGTGCTGAAATTAGAGATTCCGGTGCCAGGTCGTTGGAAAGTTCGATCGATCCTTCCAACAGTTCACGCAGGCCTCATCTCTAA

Protein Analysis

186

Amino Acids

20.37

Weight (kDa)

8.71

Isoelectric Point (pI)

57.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015289)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27050
fragaria_vesca FvH4_4g32080
malus_domestica MD13G1079700.v1.1 MD13G1172800.v1.1
prunus_persica Prupe.1G267100_v2.0.a1
pyrus_communis pycom03g02960
rosa_chinensis RchiOBHm_Chr4g0440771
rosa_laevigata RLG00000006155
rosa_roxburghii Rroxscaffold_5G00381530
rosa_rugosa Rorug04G0327800
rosa_samantha Rh4AG379600 Rh4BG392500 Rh4CG407300 Rh4DG386300
rosa_wichuraiana Rw4G032650 Rw4G032680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 412
Acc36I ACCTGC 1 cut(s) 412
AccB1I GGYRCC 1 cut(s) 497
AccBSI CCGCTC 1 cut(s) 356
AccIII TCCGGA 1 cut(s) 293
AciI CCGC 5 cut(s) 132, 242, 354, 363, 457
AclWI GGATC 2 cut(s) 78, 518
AcoI YGGCCR 1 cut(s) 431
AfiI CCNNNNNNNGG 5 cut(s) 258, 259, 392, 456, 501
AgsI TTSAA 2 cut(s) 67, 107
AjnI CCWGG 1 cut(s) 500
AluBI AGCT 2 cut(s) 214, 311
AluI AGCT 2 cut(s) 214, 311
Alw21I GWGCWC 2 cut(s) 18, 313
Alw26I GTCTC 1 cut(s) 217
Alw44I GTGCAC 1 cut(s) 14
AlwI GGATC 2 cut(s) 78, 518
Ama87I CYCGRG 1 cut(s) 306
Aor13HI TCCGGA 1 cut(s) 293
AoxI GGCC 2 cut(s) 431, 545
ApaLI GTGCAC 1 cut(s) 14
ApeKI GCWGC 1 cut(s) 371
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 2 cut(s) 184, 391
AvaI CYCGRG 1 cut(s) 306
BaeGI GKGCMC 1 cut(s) 18
BanI GGYRCC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 313
Bbv12I GWGCWC 2 cut(s) 18, 313
BbvI GCAGC 1 cut(s) 358
BccI CCATC 2 cut(s) 394, 410
BcgI CGANNNNNNTGC 2 cut(s) 108, 142
BciT130I CCWGG 1 cut(s) 502
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 419
BfuAI ACCTGC 1 cut(s) 412
BisI GCNGC 2 cut(s) 363, 372
BlsI GCNGC 2 cut(s) 364, 373
Bme1390I CCNGG 2 cut(s) 260, 502
BmeT110I CYCGRG 1 cut(s) 306
BmiI GGNNCC 2 cut(s) 298, 499
BmrFI CCNGG 2 cut(s) 260, 502
BmrI ACTGGG 1 cut(s) 445
BmuI ACTGGG 1 cut(s) 445
BpuEI CTTGAG 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 260
Bsa29I ATCGAT 1 cut(s) 522
BsaWI WCCGGW 2 cut(s) 293, 494
Bsc4I CCNNNNNNNGG 5 cut(s) 258, 259, 392, 456, 501
Bse1I ACTGG 1 cut(s) 451
Bse3DI GCAATG 1 cut(s) 366
BseAI TCCGGA 1 cut(s) 293
BseBI CCWGG 1 cut(s) 502
BseCI ATCGAT 1 cut(s) 522
BseGI GGATG 1 cut(s) 290
BseLI CCNNNNNNNGG 5 cut(s) 258, 259, 392, 456, 501
BseMI GCAATG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 18
BseXI GCAGC 1 cut(s) 358
Bsh1285I CGRYCG 2 cut(s) 132, 522
BshFI GGCC 2 cut(s) 433, 547
BshNI GGYRCC 1 cut(s) 497
BshVI ATCGAT 1 cut(s) 522
BsiEI CGRYCG 2 cut(s) 132, 522
BsiHKAI GWGCWC 2 cut(s) 18, 313
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 3 cut(s) 259, 294, 495
BslFI GGGAC 2 cut(s) 169, 262
BslI CCNNNNNNNGG 5 cut(s) 258, 259, 392, 456, 501
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 2 cut(s) 169, 262
BsnI GGCC 2 cut(s) 433, 547
BsoBI CYCGRG 1 cut(s) 306
Bsp1286I GDGCHC 2 cut(s) 18, 313
Bsp13I TCCGGA 1 cut(s) 293
Bsp143I GATC 3 cut(s) 70, 519, 523
BspACI CCGC 5 cut(s) 132, 242, 354, 363, 457
BspANI GGCC 2 cut(s) 433, 547
BspDI ATCGAT 1 cut(s) 522
BspEI TCCGGA 1 cut(s) 293
BspLI GGNNCC 2 cut(s) 298, 499
BspMAI CTGCAG 1 cut(s) 423
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 2 cut(s) 78, 518
BspT107I GGYRCC 1 cut(s) 497
BsrBI CCGCTC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 366
BsrI ACTGG 1 cut(s) 451
BssMI GATC 3 cut(s) 70, 519, 523
Bst2UI CCWGG 1 cut(s) 502
Bst4CI ACNGT 4 cut(s) 116, 273, 341, 536
Bst6I CTCTTC 2 cut(s) 194, 213
BstC8I GCNNGC 3 cut(s) 18, 45, 545
BstDEI CTNAG 1 cut(s) 55
BstF5I GGATG 1 cut(s) 290
BstKTI GATC 3 cut(s) 73, 522, 526
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 3 cut(s) 70, 519, 523
BstMCI CGRYCG 2 cut(s) 132, 522
BstMWI GCNNNNNNNGC 5 cut(s) 131, 317, 362, 368, 371
BstNI CCWGG 1 cut(s) 502
BstSCI CCNGG 2 cut(s) 258, 500
BstSFI CTRYAG 1 cut(s) 419
BstSLI GKGCMC 1 cut(s) 18
BstV1I GCAGC 1 cut(s) 358
BstXI CCANNNNNNTGG 1 cut(s) 508
Bsu15I ATCGAT 1 cut(s) 522
BsuRI GGCC 2 cut(s) 433, 547
BsuTUI ATCGAT 1 cut(s) 522
BtsCI GGATG 1 cut(s) 290
BtsIMutI CAGTG 1 cut(s) 458
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 3 cut(s) 18, 45, 545
ClaI ATCGAT 1 cut(s) 522
CviAII CATG 2 cut(s) 24, 209
CviJI RGCY 8 cut(s) 47, 125, 214, 311, 320, 362, 433, 547
CviKI_1 RGCY 8 cut(s) 47, 125, 214, 311, 320, 362, 433, 547
DdeI CTNAG 1 cut(s) 55
DpnI GATC 3 cut(s) 72, 521, 525
DpnII GATC 3 cut(s) 70, 519, 523
EaeI YGGCCR 1 cut(s) 431
Eam1104I CTCTTC 2 cut(s) 194, 213
EarI CTCTTC 2 cut(s) 194, 213
EciI GGCGGA 1 cut(s) 472
Ecl136II GAGCTC 1 cut(s) 311
Eco147I AGGCCT 1 cut(s) 547
Eco24I GRGCYC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 311
Eco88I CYCGRG 1 cut(s) 306
EcoICRI GAGCTC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 500
EcoT22I ATGCAT 2 cut(s) 6, 97
EcoT38I GRGCYC 1 cut(s) 313
FaeI CATG 2 cut(s) 27, 212
FaiI YATR 5 cut(s) 25, 97, 169, 171, 210
FaqI GGGAC 2 cut(s) 169, 262
FatI CATG 2 cut(s) 23, 208
FauI CCCGC 1 cut(s) 249
FauNDI CATATG 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 363, 372
FokI GGATG 1 cut(s) 277
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 2 cut(s) 363, 372
FspBI CTAG 1 cut(s) 215
GluI GCNGC 2 cut(s) 363, 372
HaeIII GGCC 2 cut(s) 433, 547
HapII CCGG 3 cut(s) 259, 294, 495
Hin1II CATG 2 cut(s) 27, 212
HinfI GANTC 4 cut(s) 350, 407, 440, 491
HpaII CCGG 3 cut(s) 259, 294, 495
HphI GGTGA 2 cut(s) 184, 391
Hpy166II GTNNAC 2 cut(s) 16, 539
Hpy188III TCNNGA 3 cut(s) 107, 294, 380
Hpy8I GTNNAC 2 cut(s) 16, 539
Hpy99I CGWCG 2 cut(s) 277, 439
HpyAV CCTTC 3 cut(s) 61, 454, 537
HpyCH4III ACNGT 4 cut(s) 116, 273, 341, 536
HpyCH4V TGCA 5 cut(s) 4, 16, 95, 119, 421
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 317, 362, 368, 371
HpyF3I CTNAG 1 cut(s) 55
Hsp92II CATG 2 cut(s) 27, 212
Kpn2I TCCGGA 1 cut(s) 293
Kzo9I GATC 3 cut(s) 70, 519, 523
Lsp1109I GCAGC 1 cut(s) 358
MaeI CTAG 1 cut(s) 215
MalI GATC 3 cut(s) 72, 521, 525
MbiI CCGCTC 1 cut(s) 356
MboI GATC 3 cut(s) 70, 519, 523
MboII GAAGA 3 cut(s) 46, 181, 230
MhlI GDGCHC 2 cut(s) 18, 313
MluCI AATT 1 cut(s) 483
MlyI GAGTC 2 cut(s) 344, 434
MmeI TCCRAC 2 cut(s) 489, 555
Mph1103I ATGCAT 2 cut(s) 6, 97
MroI TCCGGA 1 cut(s) 293
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 3 cut(s) 259, 294, 495
MspR9I CCNGG 2 cut(s) 260, 502
MvaI CCWGG 1 cut(s) 502
MwoI GCNNNNNNNGC 5 cut(s) 131, 317, 362, 368, 371
NciI CCSGG 1 cut(s) 260
NdeI CATATG 1 cut(s) 169
NdeII GATC 3 cut(s) 70, 519, 523
NlaIII CATG 2 cut(s) 27, 212
NlaIV GGNNCC 2 cut(s) 298, 499
NsiI ATGCAT 2 cut(s) 6, 97
PaqCI CACCTGC 1 cut(s) 412
PceI AGGCCT 1 cut(s) 547
PfeI GAWTC 2 cut(s) 407, 491
PkrI GCNGC 2 cut(s) 364, 373
Ple19I CGATCG 1 cut(s) 522
PleI GAGTC 2 cut(s) 344, 434
PpsI GAGTC 2 cut(s) 344, 434
Psp124BI GAGCTC 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 500
PspGI CCWGG 1 cut(s) 500
PspN4I GGNNCC 2 cut(s) 298, 499
PstI CTGCAG 1 cut(s) 423
PvuI CGATCG 1 cut(s) 522
RseI CAYNNNNRTG 1 cut(s) 78
SacI GAGCTC 1 cut(s) 313
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 2 cut(s) 363, 372
Sau3AI GATC 3 cut(s) 70, 519, 523
SchI GAGTC 2 cut(s) 344, 434
ScrFI CCNGG 2 cut(s) 260, 502
SduI GDGCHC 2 cut(s) 18, 313
SetI ASST 6 cut(s) 197, 216, 313, 338, 426, 506
SfcI CTRYAG 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 1 cut(s) 483
SseBI AGGCCT 1 cut(s) 547
SsiI CCGC 5 cut(s) 132, 242, 354, 363, 457
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 313
StuI AGGCCT 1 cut(s) 547
StyD4I CCNGG 2 cut(s) 258, 500
TaaI ACNGT 4 cut(s) 116, 273, 341, 536
TaqI TCGA 7 cut(s) 128, 264, 313, 381, 405, 518, 522
TasI AATT 1 cut(s) 483
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 2 cut(s) 407, 491
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 1 cut(s) 458
TseI GCWGC 1 cut(s) 371
TspDTI ATGAA 1 cut(s) 12
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 1 cut(s) 458
VneI GTGCAC 1 cut(s) 14
XspI CTAG 1 cut(s) 215
Zsp2I ATGCAT 2 cut(s) 6, 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.