Rw4G032680

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015289
Physical Location & Seq
Forward (+)
59779475 .. 59780726
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032680.1

Sequence Viewer

Length: 612 bp
ATGAACCGCAAGAGAAATAAACCCTACTTCTCTTCCCGCGGCGCCCCCGCCTCCGCATCCAAACGGCGTCGTCCCCTCCCCCCTCACGCGGAGGACCCCGAAGAAGCCGAGCTGGACAAGCCCACCTTCAAGCCGCCGCCGCCGCCCGCTCTGGTCGTAATTGGTCTCCCGCCGGACTCCTCCGTCCTCGACCTCAAGTCCCGCTTCGAGATCTACGGCCCCATTTCCCGCATCCGCATCGATCGCGACGCCGTCGGCCACGTGGCATTCCGCACCGCCGACGCTGCCCAAGCCGCCGTGGACGCCGCCCTCGACTCCTCCTTCGGCATTACCCTCCACTCCAAAAAGTTACAGGTACTGTGGGCAACAGACCCACTGGCACAGTGGCGGAAAGGAGTTGCGGTGGATGATGCTAATAGGGATTCGAGTAATGAGACGTCGTCGTCTTCCAAGCTTTTGAGGGCTGGGATGCCACTGAGAAGTCATGGCAGAGGTAACAAGCTTGCTTCGGCTATAGTCAACCCAAGAGGAGCTGCTGAGAGTTCTGAATCGTCAGTGTTGGAGGTGCCTTCGAGTGCAAGAGAGATTGTGGCTTATGATGACATTCTGTAA

Protein Analysis

203

Amino Acids

21.91

Weight (kDa)

9.43

Isoelectric Point (pI)

56.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 51 - 102 2.8e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015289)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27050
fragaria_vesca FvH4_4g32080
malus_domestica MD13G1079700.v1.1 MD13G1172800.v1.1
prunus_persica Prupe.1G267100_v2.0.a1
pyrus_communis pycom03g02960
rosa_chinensis RchiOBHm_Chr4g0440771
rosa_laevigata RLG00000006155
rosa_roxburghii Rroxscaffold_5G00381530
rosa_rugosa Rorug04G0327800
rosa_samantha Rh4AG379600 Rh4BG392500 Rh4CG407300 Rh4DG386300
rosa_wichuraiana Rw4G032650 Rw4G032680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 182, 442
AatII GACGTC 1 cut(s) 440
AccB1I GGYRCC 2 cut(s) 41, 565
AccBSI CCGCTC 1 cut(s) 149
AccII CGCG 3 cut(s) 39, 89, 246
AcoI YGGCCR 1 cut(s) 256
AcvI CACGTG 1 cut(s) 262
AcyI GRCGYC 5 cut(s) 42, 67, 249, 303, 437
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 1 cut(s) 130
AhdI GACNNNNNGTC 1 cut(s) 196
AluBI AGCT 4 cut(s) 112, 454, 502, 533
AluI AGCT 4 cut(s) 112, 454, 502, 533
Alw26I GTCTC 2 cut(s) 170, 428
AlwNI CAGNNNCTG 1 cut(s) 358
AoxI GGCC 2 cut(s) 217, 256
ApeKI GCWGC 2 cut(s) 284, 533
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 2 cut(s) 94, 218
AvaII GGWCC 1 cut(s) 94
BanI GGYRCC 2 cut(s) 41, 565
BbrPI CACGTG 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 271, 520
BceAI ACGGC 4 cut(s) 80, 232, 236, 281
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BcoDI GTCTC 2 cut(s) 170, 428
BfmI CTRYAG 1 cut(s) 513
BfoI RGCGCY 1 cut(s) 45
BglII AGATCT 1 cut(s) 210
BisI GCNGC 9 cut(s) 40, 134, 137, 140, 143, 285, 294, 306, 534
BlsI GCNGC 9 cut(s) 41, 135, 138, 141, 144, 286, 295, 307, 535
Bme18I GGWCC 1 cut(s) 94
BmeRI GACNNNNNGTC 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 94, 218
BmiI GGNNCC 4 cut(s) 43, 96, 220, 567
BmsI GCATC 5 cut(s) 65, 240, 246, 400, 459
BpiI GAAGAC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 179
Bsa29I ATCGAT 1 cut(s) 240
BsaAI YACGTR 1 cut(s) 262
BsaHI GRCGYC 5 cut(s) 42, 67, 249, 303, 437
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 37, 297
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 381
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 37, 297
BseGI GGATG 4 cut(s) 56, 231, 412, 474
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 2 cut(s) 467, 528
BseNI ACTGG 1 cut(s) 381
BseRI GAGGAG 3 cut(s) 169, 307, 543
BseXI GCAGC 2 cut(s) 271, 520
BseYI CCCAGC 1 cut(s) 464
Bsh1236I CGCG 3 cut(s) 39, 89, 246
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 2 cut(s) 219, 258
BshNI GGYRCC 2 cut(s) 41, 565
BshVI ATCGAT 1 cut(s) 240
BsiEI CGRYCG 1 cut(s) 244
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 2 cut(s) 57, 184
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 2 cut(s) 170, 428
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 2 cut(s) 57, 184
BsmI GAATGC 1 cut(s) 266
BsnI GGCC 2 cut(s) 219, 258
Bso31I GGTCTC 1 cut(s) 170
Bsp143I GATC 2 cut(s) 210, 241
Bsp68I TCGCGA 1 cut(s) 246
BspANI GGCC 2 cut(s) 219, 258
BspCNI CTCAG 2 cut(s) 468, 529
BspDI ATCGAT 1 cut(s) 240
BspFNI CGCG 3 cut(s) 39, 89, 246
BspLI GGNNCC 4 cut(s) 43, 96, 220, 567
BspT107I GGYRCC 2 cut(s) 41, 565
BspTNI GGTCTC 1 cut(s) 170
BsrBI CCGCTC 1 cut(s) 149
BsrI ACTGG 1 cut(s) 381
BssECI CCNNGG 2 cut(s) 37, 297
BssMI GATC 2 cut(s) 210, 241
BssNI GRCGYC 5 cut(s) 42, 67, 249, 303, 437
Bst4CI ACNGT 2 cut(s) 360, 384
Bst6I CTCTTC 1 cut(s) 37
BstACI GRCGYC 5 cut(s) 42, 67, 249, 303, 437
BstBAI YACGTR 1 cut(s) 262
BstC8I GCNNGC 2 cut(s) 147, 504
BstDEI CTNAG 2 cut(s) 476, 537
BstDSI CCRYGG 2 cut(s) 37, 297
BstF5I GGATG 4 cut(s) 56, 231, 412, 474
BstFNI CGCG 3 cut(s) 39, 89, 246
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 2 cut(s) 213, 244
BstMAI GTCTC 2 cut(s) 170, 428
BstMBI GATC 2 cut(s) 210, 241
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 8 cut(s) 118, 139, 142, 243, 284, 290, 293, 302
BstSFI CTRYAG 1 cut(s) 513
BstUI CGCG 3 cut(s) 39, 89, 246
BstV1I GCAGC 2 cut(s) 271, 520
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 240
BsuRI GGCC 2 cut(s) 219, 258
BsuTUI ATCGAT 1 cut(s) 240
BtgI CCRYGG 2 cut(s) 37, 297
BtsCI GGATG 4 cut(s) 56, 231, 412, 474
BtsIMutI CAGTG 4 cut(s) 374, 389, 473, 561
BtuMI TCGCGA 1 cut(s) 246
Cac8I GCNNGC 2 cut(s) 147, 504
CaiI CAGNNNCTG 1 cut(s) 358
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 2 cut(s) 94, 218
Cfr42I CCGCGG 1 cut(s) 40
ClaI ATCGAT 1 cut(s) 240
CseI GACGC 4 cut(s) 56, 257, 290, 311
Csp6I GTAC 1 cut(s) 356
CviAII CATG 1 cut(s) 485
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 2 cut(s) 476, 537
DinI GGCGCC 1 cut(s) 43
DpnI GATC 2 cut(s) 212, 243
DpnII GATC 2 cut(s) 210, 241
DrdI GACNNNNNNGTC 2 cut(s) 182, 442
DriI GACNNNNNGTC 1 cut(s) 196
DseDI GACNNNNNNGTC 2 cut(s) 182, 442
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 37
Eam1105I GACNNNNNGTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 37
EciI GGCGGA 1 cut(s) 403
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 94
Eco72I CACGTG 1 cut(s) 262
EcoO109I RGGNCCY 1 cut(s) 94
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 1 cut(s) 488
FaiI YATR 3 cut(s) 486, 515, 597
FalI AAGNNNNNCTT 4 cut(s) 110, 142, 188, 220
FaqI GGGAC 2 cut(s) 57, 184
FatI CATG 1 cut(s) 484
FauI CCCGC 6 cut(s) 44, 55, 154, 177, 209, 236
Fnu4HI GCNGC 9 cut(s) 40, 134, 137, 140, 143, 285, 294, 306, 534
FokI GGATG 4 cut(s) 43, 218, 419, 481
Fsp4HI GCNGC 9 cut(s) 40, 134, 137, 140, 143, 285, 294, 306, 534
GlaI GCGC 1 cut(s) 43
GluI GCNGC 9 cut(s) 40, 134, 137, 140, 143, 285, 294, 306, 534
GsaI CCCAGC 1 cut(s) 468
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 2 cut(s) 219, 258
HapII CCGG 1 cut(s) 173
HgaI GACGC 4 cut(s) 56, 257, 290, 311
HhaI GCGC 1 cut(s) 44
Hin1I GRCGYC 5 cut(s) 42, 67, 249, 303, 437
Hin1II CATG 1 cut(s) 488
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 2 cut(s) 452, 500
HinfI GANTC 4 cut(s) 176, 314, 422, 548
HpaII CCGG 1 cut(s) 173
Hpy166II GTNNAC 2 cut(s) 301, 520
Hpy188I TCNGA 1 cut(s) 547
Hpy188III TCNNGA 2 cut(s) 208, 245
Hpy8I GTNNAC 2 cut(s) 301, 520
Hpy99I CGWCG 6 cut(s) 72, 251, 257, 284, 442, 445
HpyAV CCTTC 3 cut(s) 136, 331, 579
HpyCH4III ACNGT 2 cut(s) 360, 384
HpyCH4IV ACGT 2 cut(s) 261, 437
HpyCH4V TGCA 1 cut(s) 578
HpyF10VI GCNNNNNNNGC 8 cut(s) 118, 139, 142, 243, 284, 290, 293, 302
HpyF3I CTNAG 2 cut(s) 476, 537
HpySE526I ACGT 2 cut(s) 261, 437
Hsp92I GRCGYC 5 cut(s) 42, 67, 249, 303, 437
Hsp92II CATG 1 cut(s) 488
HspAI GCGC 1 cut(s) 42
KasI GGCGCC 1 cut(s) 41
KspI CCGCGG 1 cut(s) 40
Kzo9I GATC 2 cut(s) 210, 241
LmnI GCTCC 1 cut(s) 530
LpnPI CCDG 6 cut(s) 98, 137, 186, 338, 362, 450
Lsp1109I GCAGC 2 cut(s) 271, 520
LweI GCATC 5 cut(s) 65, 240, 246, 400, 459
MaeII ACGT 2 cut(s) 261, 437
MaeIII GTNAC 2 cut(s) 348, 494
MalI GATC 2 cut(s) 212, 243
MbiI CCGCTC 1 cut(s) 149
MboI GATC 2 cut(s) 210, 241
MboII GAAGA 3 cut(s) 24, 113, 438
MflI RGATCY 1 cut(s) 210
MluCI AATT 1 cut(s) 159
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 2 cut(s) 170, 308
MmeI TCCRAC 1 cut(s) 540
MspA1I CMGCKG 1 cut(s) 39
MspI CCGG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 266
MvnI CGCG 3 cut(s) 39, 89, 246
MwoI GCNNNNNNNGC 8 cut(s) 118, 139, 142, 243, 284, 290, 293, 302
NarI GGCGCC 1 cut(s) 42
NdeII GATC 2 cut(s) 210, 241
NlaIII CATG 1 cut(s) 488
NlaIV GGNNCC 4 cut(s) 43, 96, 220, 567
NmeAIII GCCGAG 1 cut(s) 133
NruI TCGCGA 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 266
PfeI GAWTC 2 cut(s) 422, 548
PflFI GACNNNGTC 2 cut(s) 251, 439
PkrI GCNGC 9 cut(s) 41, 135, 138, 141, 144, 286, 295, 307, 535
Ple19I CGATCG 1 cut(s) 244
PleI GAGTC 2 cut(s) 170, 308
PluTI GGCGCC 1 cut(s) 45
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 2 cut(s) 170, 308
Ppu21I YACGTR 1 cut(s) 262
PpuMI RGGWCCY 1 cut(s) 94
Psp5II RGGWCCY 1 cut(s) 94
PspCI CACGTG 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 464
PspN4I GGNNCC 4 cut(s) 43, 96, 220, 567
PspPI GGNCC 2 cut(s) 94, 218
PspPPI RGGWCCY 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 358
PsuI RGATCY 1 cut(s) 210
PsyI GACNNNGTC 2 cut(s) 251, 439
PvuI CGATCG 1 cut(s) 244
RruI TCGCGA 1 cut(s) 246
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
SacII CCGCGG 1 cut(s) 40
SatI GCNGC 9 cut(s) 40, 134, 137, 140, 143, 285, 294, 306, 534
Sau3AI GATC 2 cut(s) 210, 241
Sau96I GGNCC 2 cut(s) 94, 218
SchI GAGTC 2 cut(s) 170, 308
SfaNI GCATC 5 cut(s) 65, 240, 246, 400, 459
SfcI CTRYAG 1 cut(s) 513
SfoI GGCGCC 1 cut(s) 43
Sfr303I CCGCGG 1 cut(s) 40
SgrBI CCGCGG 1 cut(s) 40
SinI GGWCC 1 cut(s) 94
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 1 cut(s) 159
SspDI GGCGCC 1 cut(s) 41
TaaI ACNGT 2 cut(s) 360, 384
TaiI ACGT 2 cut(s) 264, 440
TaqI TCGA 6 cut(s) 189, 207, 240, 312, 425, 572
TasI AATT 1 cut(s) 159
TauI GCSGC 7 cut(s) 42, 136, 139, 142, 145, 296, 308
TfiI GAWTC 2 cut(s) 422, 548
TscAI CASTG 4 cut(s) 381, 389, 480, 561
TseI GCWGC 2 cut(s) 284, 533
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 4 cut(s) 381, 389, 480, 561
Tth111I GACNNNGTC 2 cut(s) 251, 439
VpaK11BI GGWCC 1 cut(s) 94
XcmI CCANNNNNNNNNTGG 1 cut(s) 381
ZraI GACGTC 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.