FvH4_4g32080

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015289
Physical Location & Seq
Forward (+)
30900639 .. 30901966
1328 bp
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UTR
Exon/CDS
Intron
FvH4_4g32080.t1

Sequence Viewer

Length: 609 bp
ATGAACCGGAAGAGAGATAAACCCTACTTCTCCAACCGCCGCGCCCCCGCCTCGGCTCCAAAACGACGTCGTCCTCTCCCCCCTCACACCGAAGAACCCGAGCTGCTTGACAAGCCCACCTTCAAACCGCCGCCGCCGCCCGCTCTCGTCGTACTCGGTCTCCCGCCGGACTCCTCCGTCCTCGACCTCAAGTCCCGCTTCGAGATCTACGGCCCCATCTCCCGCATCCGCATCGACCGCGACTCCATCGGCCACGTGGCATTCCGCACCGCCGACGCTGCCCAAGCCGCCGTTGACGCCGCCCTCGATTCCGCCTTCGGCATTACCCTCCACTCCAAAAAGTTACAGGTGTTGTGGGCAACAGACCCACTGGCACAGTGGAGGAAAGGAGTTGCGGTTGATGAGGCTAATAGGGATTCGAGTAATGAGACATCTTCTTCGTCGTCGAAGCTTTTGAGGGCTGGGATGCCACTGAGGAGACATGGCAGAGGGAATAAGCTTGCTTCGGCTATAGTGAACCCGAGAGGAGCTGCCGAGGCTTCTGAATCGTCGGTTTTGGAGGTGCCTTCCAGAGCAAGAGAGATTGTGGCTTATGATGACATTCTGTAA

Protein Analysis

203

Amino Acids

22.11

Weight (kDa)

9.98

Isoelectric Point (pI)

58.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 49 - 100 5.9e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015289)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27050
fragaria_vesca FvH4_4g32080
malus_domestica MD13G1079700.v1.1 MD13G1172800.v1.1
prunus_persica Prupe.1G267100_v2.0.a1
pyrus_communis pycom03g02960
rosa_chinensis RchiOBHm_Chr4g0440771
rosa_laevigata RLG00000006155
rosa_roxburghii Rroxscaffold_5G00381530
rosa_rugosa Rorug04G0327800
rosa_samantha Rh4AG379600 Rh4BG392500 Rh4CG407300 Rh4DG386300
rosa_wichuraiana Rw4G032650 Rw4G032680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AatII GACGTC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 562
AccBSI CCGCTC 1 cut(s) 143
AccII CGCG 2 cut(s) 42, 240
AcoI YGGCCR 1 cut(s) 250
AcvI CACGTG 1 cut(s) 256
AcyI GRCGYC 2 cut(s) 67, 297
AfaI GTAC 1 cut(s) 153
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 1 cut(s) 124
AhdI GACNNNNNGTC 1 cut(s) 190
AluBI AGCT 4 cut(s) 103, 451, 499, 530
AluI AGCT 4 cut(s) 103, 451, 499, 530
Alw26I GTCTC 3 cut(s) 164, 422, 472
Ama87I CYCGRG 2 cut(s) 98, 520
AoxI GGCC 2 cut(s) 211, 250
ApeKI GCWGC 3 cut(s) 103, 278, 530
ArsI GACNNNNNNTTYG 2 cut(s) 421, 453
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 212
AvaI CYCGRG 2 cut(s) 98, 520
BanI GGYRCC 1 cut(s) 562
BbrPI CACGTG 1 cut(s) 256
BbvI GCAGC 3 cut(s) 90, 265, 517
BccI CCATC 2 cut(s) 224, 254
BceAI ACGGC 2 cut(s) 226, 275
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BcoDI GTCTC 3 cut(s) 164, 422, 472
BfmI CTRYAG 1 cut(s) 510
BglII AGATCT 1 cut(s) 204
BisI GCNGC 9 cut(s) 40, 104, 131, 134, 137, 279, 288, 300, 531
BlsI GCNGC 9 cut(s) 41, 105, 132, 135, 138, 280, 289, 301, 532
BmeRI GACNNNNNGTC 1 cut(s) 190
BmeT110I CYCGRG 2 cut(s) 98, 520
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 3 cut(s) 57, 214, 564
BmsI GCATC 3 cut(s) 234, 240, 456
BpuEI CTTGAG 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 256
BsaHI GRCGYC 2 cut(s) 67, 297
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 51, 534
BsaWI WCCGGW 1 cut(s) 6
BsaXI ACNNNNNCTCC 6 cut(s) 144, 174, 227, 257, 381, 411
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse1I ACTGG 1 cut(s) 375
BseDI CCNNGG 2 cut(s) 51, 534
BseGI GGATG 2 cut(s) 225, 471
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 1 cut(s) 375
BseRI GAGGAG 3 cut(s) 163, 490, 540
BseXI GCAGC 3 cut(s) 90, 265, 517
BseYI CCCAGC 1 cut(s) 461
Bsh1236I CGCG 2 cut(s) 42, 240
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 2 cut(s) 213, 252
BshNI GGYRCC 1 cut(s) 562
BsiEI CGRYCG 1 cut(s) 238
BsiHKCI CYCGRG 2 cut(s) 98, 520
BsiSI CCGG 2 cut(s) 7, 167
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 1 cut(s) 52
BsmAI GTCTC 3 cut(s) 164, 422, 472
BsmFI GGGAC 1 cut(s) 178
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 2 cut(s) 213, 252
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 2 cut(s) 98, 520
Bsp143I GATC 1 cut(s) 204
BspANI GGCC 2 cut(s) 213, 252
BspCNI CTCAG 1 cut(s) 465
BspFNI CGCG 2 cut(s) 42, 240
BspLI GGNNCC 3 cut(s) 57, 214, 564
BspT107I GGYRCC 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 143
BsrI ACTGG 1 cut(s) 375
BssECI CCNNGG 2 cut(s) 51, 534
BssMI GATC 1 cut(s) 204
BssNI GRCGYC 2 cut(s) 67, 297
Bst4CI ACNGT 1 cut(s) 378
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 2 cut(s) 67, 297
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 2 cut(s) 141, 501
BstDEI CTNAG 1 cut(s) 473
BstF5I GGATG 2 cut(s) 225, 471
BstFNI CGCG 2 cut(s) 42, 240
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 1 cut(s) 207
BstMAI GTCTC 3 cut(s) 164, 422, 472
BstMBI GATC 1 cut(s) 204
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 8 cut(s) 112, 136, 237, 278, 284, 287, 296, 536
BstSFI CTRYAG 1 cut(s) 510
BstUI CGCG 2 cut(s) 42, 240
BstV1I GCAGC 3 cut(s) 90, 265, 517
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BsuRI GGCC 2 cut(s) 213, 252
BtsCI GGATG 2 cut(s) 225, 471
BtsIMutI CAGTG 3 cut(s) 368, 383, 470
Cac8I GCNNGC 2 cut(s) 141, 501
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 212
CseI GACGC 2 cut(s) 284, 305
Csp6I GTAC 1 cut(s) 152
CviAII CATG 1 cut(s) 482
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 1 cut(s) 473
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
DrdI GACNNNNNNGTC 1 cut(s) 176
DriI GACNNNNNGTC 1 cut(s) 190
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 5
Eam1105I GACNNNNNGTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 5
EciI GGCGGA 1 cut(s) 301
Eco31I GGTCTC 1 cut(s) 164
Eco72I CACGTG 1 cut(s) 256
Eco88I CYCGRG 2 cut(s) 98, 520
FaeI CATG 1 cut(s) 485
FaiI YATR 3 cut(s) 483, 512, 594
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 182, 214
FaqI GGGAC 1 cut(s) 178
FatI CATG 1 cut(s) 481
FauI CCCGC 5 cut(s) 55, 148, 171, 203, 230
Fnu4HI GCNGC 9 cut(s) 40, 104, 131, 134, 137, 279, 288, 300, 531
FokI GGATG 2 cut(s) 212, 478
Fsp4HI GCNGC 9 cut(s) 40, 104, 131, 134, 137, 279, 288, 300, 531
GlaI GCGC 1 cut(s) 43
GluI GCNGC 9 cut(s) 40, 104, 131, 134, 137, 279, 288, 300, 531
GsaI CCCAGC 1 cut(s) 465
HaeIII GGCC 2 cut(s) 213, 252
HapII CCGG 2 cut(s) 7, 167
HgaI GACGC 2 cut(s) 284, 305
HhaI GCGC 1 cut(s) 44
Hin1I GRCGYC 2 cut(s) 67, 297
Hin1II CATG 1 cut(s) 485
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 2 cut(s) 449, 497
HinfI GANTC 5 cut(s) 170, 242, 308, 416, 545
HpaII CCGG 2 cut(s) 7, 167
Hpy166II GTNNAC 2 cut(s) 295, 517
Hpy188I TCNGA 1 cut(s) 544
Hpy188III TCNNGA 2 cut(s) 202, 570
Hpy8I GTNNAC 2 cut(s) 295, 517
Hpy99I CGWCG 7 cut(s) 69, 72, 152, 278, 445, 448, 553
HpyAV CCTTC 3 cut(s) 130, 325, 576
HpyCH4III ACNGT 1 cut(s) 378
HpyCH4IV ACGT 2 cut(s) 67, 255
HpyF10VI GCNNNNNNNGC 8 cut(s) 112, 136, 237, 278, 284, 287, 296, 536
HpyF3I CTNAG 1 cut(s) 473
HpySE526I ACGT 2 cut(s) 67, 255
Hsp92I GRCGYC 2 cut(s) 67, 297
Hsp92II CATG 1 cut(s) 485
HspAI GCGC 1 cut(s) 42
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 2 cut(s) 61, 527
LpnPI CCDG 6 cut(s) 20, 180, 332, 356, 447, 583
Lsp1109I GCAGC 3 cut(s) 90, 265, 517
LweI GCATC 3 cut(s) 234, 240, 456
MaeII ACGT 2 cut(s) 67, 255
MaeIII GTNAC 1 cut(s) 342
MalI GATC 1 cut(s) 206
MbiI CCGCTC 1 cut(s) 143
MboI GATC 1 cut(s) 204
MboII GAAGA 4 cut(s) 22, 104, 426, 429
MflI RGATCY 1 cut(s) 204
MlyI GAGTC 2 cut(s) 164, 236
MmeI TCCRAC 1 cut(s) 57
MspI CCGG 2 cut(s) 7, 167
Mva1269I GAATGC 1 cut(s) 260
MvnI CGCG 2 cut(s) 42, 240
MwoI GCNNNNNNNGC 8 cut(s) 112, 136, 237, 278, 284, 287, 296, 536
NdeII GATC 1 cut(s) 204
NlaIII CATG 1 cut(s) 485
NlaIV GGNNCC 3 cut(s) 57, 214, 564
NmeAIII GCCGAG 2 cut(s) 32, 559
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 1 cut(s) 260
PfeI GAWTC 3 cut(s) 308, 416, 545
PflFI GACNNNGTC 1 cut(s) 69
PkrI GCNGC 9 cut(s) 41, 105, 132, 135, 138, 280, 289, 301, 532
PleI GAGTC 2 cut(s) 164, 236
PmaCI CACGTG 1 cut(s) 256
PmlI CACGTG 1 cut(s) 256
PpsI GAGTC 2 cut(s) 164, 236
Ppu21I YACGTR 1 cut(s) 256
PspCI CACGTG 1 cut(s) 256
PspFI CCCAGC 1 cut(s) 461
PspN4I GGNNCC 3 cut(s) 57, 214, 564
PspPI GGNCC 1 cut(s) 212
PsuI RGATCY 1 cut(s) 204
PsyI GACNNNGTC 1 cut(s) 69
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
SatI GCNGC 9 cut(s) 40, 104, 131, 134, 137, 279, 288, 300, 531
Sau3AI GATC 1 cut(s) 204
Sau96I GGNCC 1 cut(s) 212
SchI GAGTC 2 cut(s) 164, 236
SfaNI GCATC 3 cut(s) 234, 240, 456
SfcI CTRYAG 1 cut(s) 510
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 378
TaiI ACGT 2 cut(s) 70, 258
TaqI TCGA 6 cut(s) 183, 201, 234, 306, 419, 446
TaqII GACCGA 1 cut(s) 146
TauI GCSGC 6 cut(s) 42, 133, 136, 139, 290, 302
TfiI GAWTC 3 cut(s) 308, 416, 545
TscAI CASTG 3 cut(s) 375, 383, 477
TseI GCWGC 3 cut(s) 103, 278, 530
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 3 cut(s) 375, 383, 477
Tth111I GACNNNGTC 1 cut(s) 69
XcmI CCANNNNNNNNNTGG 2 cut(s) 253, 375
ZraI GACGTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.