AT1G29200

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006470
Physical Location & Seq
Forward (+)
10207577 .. 10210622
3046 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G29200.2

Sequence Viewer

Length: 1488 bp
ATGTCAATTAAGAATGGAGCAGTAGATGCTTCACCAGTTCAAATGTATAGCCGTCTTCTAAACTTAGCTTCGGATTCTCTTGCAAAGAATGAATTCAAACCCGATACACCAAACTTCCGGGAGGAAAGATCTTCAAAGTCATCACAGTGGAAGCCTTGTGCAGATAATAACAAAGCAGCTGTGGCATTAGAGAGGTCTAGAGAATTAAGCAATGGTTACATCATGGTTAGTGCAAATGGAGGCCTTAATCAACAGCGCGTGGCTATCTGTAACGCTGTTGCGGTTGCTGCTCTGCTCAACGCGACTCTAGTTCTTCCTCGGTTTCTTTACAGCAATGTATGGAAAGATCCTAGCCAATTTGGTGATATATACCAAGAGGATCATTTTATCGAGTATCTGAAAGATGAGGTGAATATAGTGAAGAATCTTCCTCAACATCTAAAATCTACTGATAACAAGAATCTCAGCTTGGTTACTGATACGGAGCTTGTAAAAGAGGCAACACCAGTCGATTACATCGAACATGTTCTTCCTTTGTTAAAGAAGTATGGAATGGTTCATCTTTTCGGATACGGGAATCGCCTCGGGTTTGATCCATTGCCTTTTGACGTTCAGAGATTACGATGTAAATGCAACTTTCACGCGCTGAAATTCGCGCCCAAGATTCAAGAAGCGGGATCTCTGTTGGTGAAACGGATAAGAAGATTCAAAACATCGCGTAGCAGATTGGAAGAAGCATTACTTGGTGAATCCATGGTGAAAAGTACTGTTAAAGGAGAAGAAGAACCGTTGAAGTATCTTGCTTTGCATCTAAGGTTTGAAGAAGACATGGTTGCTTATTCTCTATGCGATTTTGGAGGAGGAGAAGCCGAGCGCAAAGAGCTTCAAGCTTACCGTGAAGATCATTTCCCTCTTCTACTCAAACGTTTGAAGAAATCAAAGCCAGTGTCCCCAGAAGAGTTAAGAAAAACGGGCAAGTGTCCACTAACACCAGAGGAAGCAACTCTGGTTCTTGCTGGTCTTGGATTCAAGAGAAAAACATATATTTACTTAGCTGGATCTCAAATCTATGGAGGCTCTTCTCGTATGCTTCCTTTAACAAGACTGTACCCGAATATCGCGACCAAAGAAACTCTCCTTACACCTCAAGAACTTGCGCCATTCAAGAACTTCTCTTCTCAGCTAGCTGCACTAGATTTCATAGCTTGTATAGCATCAGATGTCTTTGCAATGACTGATTCTGGGAGTCAACTATCATCCTTAGTGTCTGGATTTAGAAACTACTACGGAAACGGTCAGGCACCAACATTGAGACCTAACAAGAAGAGGCTTGCAGCAATCTTATCAGACAGTGAAACAATAAAGTGGAAGATTTTTGAAGATCGTGTGAGAAAAATGGTAGAGGAAGGCCAAAAGCTTCGTACTAGGCCTTATGGACGCAGCATTTATAGACAACCGAGATGTCCTGAGTGTATGTGTAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

495

Amino Acids

55.98

Weight (kDa)

9.28

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 189 - 545 6.4e-98 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1300
AccII CGCG 6 cut(s) 258, 302, 644, 656, 718, 1121
AciI CCGC 2 cut(s) 281, 674
AclI AACGTT 1 cut(s) 925
AclWI GGATC 5 cut(s) 341, 387, 587, 685, 1066
AcsI RAATTY 3 cut(s) 92, 650, 1481
AfaI GTAC 3 cut(s) 766, 1109, 1423
AflIII ACRYGT 1 cut(s) 523
AjuI GAANNNNNNNTTGG 4 cut(s) 452, 484, 726, 758
Alw26I GTCTC 1 cut(s) 1306
AlwI GGATC 5 cut(s) 341, 387, 587, 685, 1066
Ama87I CYCGRG 1 cut(s) 584
AoxI GGCC 3 cut(s) 241, 1408, 1427
ApeKI GCWGC 5 cut(s) 176, 287, 1187, 1334, 1440
ApoI RAATTY 3 cut(s) 92, 650, 1481
ArsI GACNNNNNNTTYG 2 cut(s) 932, 964
Asp700I GAANNNNTTC 2 cut(s) 92, 525
AspLEI GCGC 5 cut(s) 258, 646, 658, 876, 1159
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 6 cut(s) 24, 374, 421, 700, 758, 769
AsuNHI GCTAGC 1 cut(s) 1183
AvaI CYCGRG 1 cut(s) 584
BanI GGYRCC 1 cut(s) 1300
BarI GAAGNNNNNNTAC 2 cut(s) 723, 755
BbsI GAAGAC 2 cut(s) 47, 831
BbvI GCAGC 5 cut(s) 188, 274, 1174, 1346, 1452
BceAI ACGGC 1 cut(s) 36
BcgI CGANNNNNNTGC 2 cut(s) 612, 646
BciVI GTATCC 1 cut(s) 563
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 1306
BfaI CTAG 6 cut(s) 198, 308, 351, 1184, 1193, 1425
BfuI GTATCC 1 cut(s) 563
BglII AGATCT 1 cut(s) 128
BisI GCNGC 5 cut(s) 177, 288, 1188, 1335, 1441
BlsI GCNGC 5 cut(s) 178, 289, 1189, 1336, 1442
BmcAI AGTACT 1 cut(s) 766
Bme1390I CCNGG 1 cut(s) 119
BmeT110I CYCGRG 1 cut(s) 584
BmiI GGNNCC 1 cut(s) 1302
BmrFI CCNGG 1 cut(s) 119
BmsI GCATC 3 cut(s) 16, 817, 1223
BmtI GCTAGC 1 cut(s) 1187
BpiI GAAGAC 2 cut(s) 47, 831
BpuEI CTTGAG 1 cut(s) 1131
BpuMI CCSGG 1 cut(s) 119
BsaI GGTCTC 1 cut(s) 1306
BsaJI CCNNGG 3 cut(s) 317, 583, 753
Bse1I ACTGG 3 cut(s) 35, 506, 944
Bse3DI GCAATG 4 cut(s) 217, 340, 596, 1236
BseDI CCNNGG 3 cut(s) 317, 583, 753
BseGI GGATG 1 cut(s) 1256
BseMI GCAATG 4 cut(s) 217, 340, 596, 1236
BseMII CTCAG 3 cut(s) 478, 1193, 1458
BseNI ACTGG 3 cut(s) 35, 506, 944
BseRI GAGGAG 2 cut(s) 873, 876
BseXI GCAGC 5 cut(s) 188, 274, 1174, 1346, 1452
BsgI GTGCAG 2 cut(s) 180, 1173
Bsh1236I CGCG 6 cut(s) 258, 302, 644, 656, 718, 1121
BshFI GGCC 3 cut(s) 243, 1410, 1429
BshNI GGYRCC 1 cut(s) 1300
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 1 cut(s) 118
BslFI GGGAC 1 cut(s) 934
BsmAI GTCTC 1 cut(s) 1306
BsmFI GGGAC 1 cut(s) 934
BsnI GGCC 3 cut(s) 243, 1410, 1429
Bso31I GGTCTC 1 cut(s) 1306
BsoBI CYCGRG 1 cut(s) 584
Bsp143I GATC 8 cut(s) 128, 346, 379, 592, 677, 901, 1058, 1381
Bsp19I CCATGG 1 cut(s) 753
Bsp68I TCGCGA 1 cut(s) 1121
BspACI CCGC 2 cut(s) 281, 674
BspANI GGCC 3 cut(s) 243, 1410, 1429
BspCNI CTCAG 3 cut(s) 477, 1192, 1459
BspFNI CGCG 6 cut(s) 258, 302, 644, 656, 718, 1121
BspLI GGNNCC 1 cut(s) 1302
BspOI GCTAGC 1 cut(s) 1187
BspPI GGATC 5 cut(s) 341, 387, 587, 685, 1066
BspQI GCTCTTC 1 cut(s) 1084
BspT107I GGYRCC 1 cut(s) 1300
BspTNI GGTCTC 1 cut(s) 1306
BsrDI GCAATG 4 cut(s) 217, 340, 596, 1236
BsrI ACTGG 3 cut(s) 35, 506, 944
BssECI CCNNGG 3 cut(s) 317, 583, 753
BssMI GATC 8 cut(s) 128, 346, 379, 592, 677, 901, 1058, 1381
BssT1I CCWWGG 1 cut(s) 753
Bst4CI ACNGT 7 cut(s) 147, 769, 789, 896, 1107, 1295, 1352
Bst6I CTCTTC 5 cut(s) 918, 951, 1084, 1180, 1319
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 1185, 1332
BstDEI CTNAG 7 cut(s) 64, 464, 812, 1051, 1179, 1261, 1467
BstDSI CCRYGG 1 cut(s) 753
BstF5I GGATG 1 cut(s) 1256
BstFNI CGCG 6 cut(s) 258, 302, 644, 656, 718, 1121
BstHHI GCGC 5 cut(s) 258, 646, 658, 876, 1159
BstKTI GATC 8 cut(s) 131, 349, 382, 595, 680, 904, 1061, 1384
BstMAI GTCTC 1 cut(s) 1306
BstMBI GATC 8 cut(s) 128, 346, 379, 592, 677, 901, 1058, 1381
BstMWI GCNNNNNNNGC 5 cut(s) 26, 182, 287, 880, 1211
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 1 cut(s) 117
BstUI CGCG 6 cut(s) 258, 302, 644, 656, 718, 1121
BstV1I GCAGC 5 cut(s) 188, 274, 1174, 1346, 1452
BstV2I GAAGAC 2 cut(s) 47, 831
BstX2I RGATCY 4 cut(s) 128, 346, 677, 1058
BstYI RGATCY 4 cut(s) 128, 346, 677, 1058
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 3 cut(s) 243, 1410, 1429
BtgI CCRYGG 1 cut(s) 753
BtgZI GCGATG 1 cut(s) 699
BtsCI GGATG 1 cut(s) 1256
BtsIMutI CAGTG 3 cut(s) 152, 951, 1357
BtuMI TCGCGA 1 cut(s) 1121
Cac8I GCNNGC 2 cut(s) 1185, 1332
CfoI GCGC 5 cut(s) 258, 646, 658, 876, 1159
CseI GACGC 1 cut(s) 1446
Csp6I GTAC 3 cut(s) 765, 1108, 1422
CviAII CATG 4 cut(s) 223, 524, 754, 829
CviQI GTAC 3 cut(s) 765, 1108, 1422
DdeI CTNAG 7 cut(s) 64, 464, 812, 1051, 1179, 1261, 1467
DpnI GATC 8 cut(s) 130, 348, 381, 594, 679, 903, 1060, 1383
DpnII GATC 8 cut(s) 128, 346, 379, 592, 677, 901, 1058, 1381
Eam1104I CTCTTC 5 cut(s) 918, 951, 1084, 1180, 1319
EarI CTCTTC 5 cut(s) 918, 951, 1084, 1180, 1319
Eco130I CCWWGG 1 cut(s) 753
Eco147I AGGCCT 2 cut(s) 243, 1429
Eco31I GGTCTC 1 cut(s) 1306
Eco88I CYCGRG 1 cut(s) 584
EcoRI GAATTC 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 753
ErhI CCWWGG 1 cut(s) 753
FaeI CATG 4 cut(s) 226, 527, 757, 832
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 934
FatI CATG 4 cut(s) 222, 523, 753, 828
FauI CCCGC 1 cut(s) 667
Fnu4HI GCNGC 5 cut(s) 177, 288, 1188, 1335, 1441
FokI GGATG 1 cut(s) 1243
Fsp4HI GCNGC 5 cut(s) 177, 288, 1188, 1335, 1441
FspBI CTAG 6 cut(s) 198, 308, 351, 1184, 1193, 1425
GlaI GCGC 5 cut(s) 257, 645, 657, 875, 1158
GluI GCNGC 5 cut(s) 177, 288, 1188, 1335, 1441
HaeIII GGCC 3 cut(s) 243, 1410, 1429
HapII CCGG 1 cut(s) 118
HgaI GACGC 1 cut(s) 1446
HhaI GCGC 5 cut(s) 258, 646, 658, 876, 1159
Hin1II CATG 4 cut(s) 226, 527, 757, 832
Hin6I GCGC 5 cut(s) 256, 644, 656, 874, 1157
HinP1I GCGC 5 cut(s) 256, 644, 656, 874, 1157
HincII GTYRAC 1 cut(s) 1250
HindII GTYRAC 1 cut(s) 1250
HindIII AAGCTT 2 cut(s) 888, 1415
HpaII CCGG 1 cut(s) 118
HphI GGTGA 6 cut(s) 24, 374, 421, 700, 758, 769
Hpy166II GTNNAC 2 cut(s) 983, 1250
Hpy188I TCNGA 6 cut(s) 73, 399, 569, 615, 1219, 1348
Hpy188III TCNNGA 8 cut(s) 198, 668, 1030, 1120, 1148, 1165, 1269, 1466
Hpy8I GTNNAC 2 cut(s) 983, 1250
HpyAV CCTTC 1 cut(s) 1400
HpyCH4III ACNGT 7 cut(s) 147, 769, 789, 896, 1107, 1295, 1352
HpyCH4IV ACGT 2 cut(s) 609, 925
HpyCH4V TGCA 8 cut(s) 83, 161, 233, 633, 808, 1190, 1229, 1334
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 182, 287, 880, 1211
HpyF3I CTNAG 7 cut(s) 64, 464, 812, 1051, 1179, 1261, 1467
HpySE526I ACGT 2 cut(s) 609, 925
Hsp92II CATG 4 cut(s) 226, 527, 757, 832
HspAI GCGC 5 cut(s) 256, 644, 656, 874, 1157
Kzo9I GATC 8 cut(s) 128, 346, 379, 592, 677, 901, 1058, 1381
LguI GCTCTTC 1 cut(s) 1084
LmnI GCTCC 2 cut(s) 17, 484
Lsp1109I GCAGC 5 cut(s) 188, 274, 1174, 1346, 1452
LweI GCATC 3 cut(s) 16, 817, 1223
MaeI CTAG 6 cut(s) 198, 308, 351, 1184, 1193, 1425
MaeII ACGT 2 cut(s) 609, 925
MaeIII GTNAC 3 cut(s) 215, 269, 472
MalI GATC 8 cut(s) 130, 348, 381, 594, 679, 903, 1060, 1383
MboI GATC 8 cut(s) 128, 346, 379, 592, 677, 901, 1058, 1381
MflI RGATCY 4 cut(s) 128, 346, 677, 1058
MluCI AATT 6 cut(s) 6, 92, 203, 356, 650, 1481
MlyI GAGTC 2 cut(s) 298, 1255
MroXI GAANNNNTTC 2 cut(s) 92, 525
MseI TTAA 7 cut(s) 9, 206, 246, 539, 771, 962, 1097
MslI CAYNNNNRTG 1 cut(s) 145
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 1 cut(s) 119
MvnI CGCG 6 cut(s) 258, 302, 644, 656, 718, 1121
MwoI GCNNNNNNNGC 5 cut(s) 26, 182, 287, 880, 1211
NciI CCSGG 1 cut(s) 119
NcoI CCATGG 1 cut(s) 753
NdeII GATC 8 cut(s) 128, 346, 379, 592, 677, 901, 1058, 1381
NheI GCTAGC 1 cut(s) 1183
NlaIII CATG 4 cut(s) 226, 527, 757, 832
NlaIV GGNNCC 1 cut(s) 1302
NmeAIII GCCGAG 1 cut(s) 895
NruI TCGCGA 1 cut(s) 1121
NspI RCATGY 1 cut(s) 527
PceI AGGCCT 2 cut(s) 243, 1429
PciI ACATGT 1 cut(s) 523
PciSI GCTCTTC 1 cut(s) 1084
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PdmI GAANNNNTTC 2 cut(s) 92, 525
PfeI GAWTC 9 cut(s) 74, 424, 460, 577, 664, 705, 749, 1026, 1238
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 5 cut(s) 178, 289, 1189, 1336, 1442
PleI GAGTC 2 cut(s) 298, 1254
PpsI GAGTC 2 cut(s) 298, 1254
PscI ACATGT 1 cut(s) 523
Psp1406I AACGTT 1 cut(s) 925
PspN4I GGNNCC 1 cut(s) 1302
PsuI RGATCY 4 cut(s) 128, 346, 677, 1058
PvuII CAGCTG 1 cut(s) 179
RruI TCGCGA 1 cut(s) 1121
RsaI GTAC 3 cut(s) 766, 1109, 1423
RsaNI GTAC 3 cut(s) 765, 1108, 1422
RseI CAYNNNNRTG 1 cut(s) 145
SapI GCTCTTC 1 cut(s) 1084
SaqAI TTAA 7 cut(s) 9, 206, 246, 539, 771, 962, 1097
SatI GCNGC 5 cut(s) 177, 288, 1188, 1335, 1441
Sau3AI GATC 8 cut(s) 128, 346, 379, 592, 677, 901, 1058, 1381
ScaI AGTACT 1 cut(s) 766
SchI GAGTC 2 cut(s) 298, 1255
ScrFI CCNGG 1 cut(s) 119
SfaNI GCATC 3 cut(s) 16, 817, 1223
SmiMI CAYNNNNRTG 1 cut(s) 145
SmlI CTYRAG 1 cut(s) 1146
SmoI CTYRAG 1 cut(s) 1146
Sse9I AATT 6 cut(s) 6, 92, 203, 356, 650, 1481
SseBI AGGCCT 2 cut(s) 243, 1429
SsiI CCGC 2 cut(s) 281, 674
SspMI CTAG 6 cut(s) 198, 308, 351, 1184, 1193, 1425
StuI AGGCCT 2 cut(s) 243, 1429
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 1 cut(s) 753
TaaI ACNGT 7 cut(s) 147, 769, 789, 896, 1107, 1295, 1352
TaiI ACGT 2 cut(s) 612, 928
TaqI TCGA 3 cut(s) 390, 510, 519
TasI AATT 6 cut(s) 6, 92, 203, 356, 650, 1481
TatI WGTACW 1 cut(s) 764
TfiI GAWTC 9 cut(s) 74, 424, 460, 577, 664, 705, 749, 1026, 1238
Tru1I TTAA 7 cut(s) 9, 206, 246, 539, 771, 962, 1097
Tru9I TTAA 7 cut(s) 9, 206, 246, 539, 771, 962, 1097
TscAI CASTG 3 cut(s) 152, 951, 1357
TseI GCWGC 5 cut(s) 176, 287, 1187, 1334, 1440
TspDTI ATGAA 3 cut(s) 105, 548, 1189
TspGWI ACGGA 3 cut(s) 497, 709, 1302
TspRI CASTG 3 cut(s) 152, 951, 1357
XapI RAATTY 3 cut(s) 92, 650, 1481
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 527
XmnI GAANNNNTTC 2 cut(s) 92, 525
XspI CTAG 6 cut(s) 198, 308, 351, 1184, 1193, 1425
ZrmI AGTACT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.