pycom05g27610

protein At1g04910-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006470
Physical Location & Seq
Forward (+)
28543007 .. 28545807
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27610.1

Sequence Viewer

Length: 1806 bp
ATGGGGAAACAAGGGTCTCCGAGAAGTCCACATCCCGAGCCACAGATTGACGATAACTTATCGTCAGGGACTAAAGACTATGATCCTTCTAATGTTGGGAGGAGAATGTCAGGAGGAGATAACCATTTGAATTCCAAAATGTTGTTCCTTCATGGTCTGAAAAATGACCATTTTCATATTGTGAGAAGTGATTCTGTAAAATATGGTACCTGTAAAGGGAGCTTCGTGGGGAAAAAGCATTTGTGGCTTCGAAAAGATGATCGATCAATCGTCATTGTGTTTGCGTTGATGGGTTTCCTTCTCCTACTTGATTCTTTTCTAGTGTCTATTTTTTATTCGATGGTTCTTCAGAATTCAACTTATTATGTAAGAAATCCACAGGGGCTTAAGGAGGACAGGGATGCCTACATTACGAAAGAAAAATCACCAGTACATATGTATGACCAGCTTCTAAAGGCAGCGAAAGATGCCATTGCAGAGTTCACTGATTTATGCGGCGTACTGATAAGCTCAGTTTGTTCATGTTATTACAGCCAATTTGAAGATATTTACCAAGAACAGTATTTTATAGATGTACTGAAGGATGAAGTAAACATAGTCAAAGAACTTCCTTCTCATTTGAAAACACTAGACGTTGAAGCAATTGGTAGCTTTGTACGTCATCCTCCTATTTTGTTACATTCATTTCCGCAGCAAATTTGTAAAATAAGTACCTTTTCCTCTTCGCAGATTACTGATGCAGATCTTGTGAAGGAGGCAAAACCAATTGATTATATCAGAATTGTACTTCCTCTTCTCTTGCGAAATCAAGTTGTTCACTTCCTCGGATATGGAAATCGACTAGGCTTTGATCCGTTGCCTTCTGAGCTTCAGAGATTAAGATGCAAATGTAACTTTCATGCTTTGAAGTTTGTGCCCAAAATCCAACAAGTCGGTTCTCTGTTGGTTAGAAGGATAAGGAATTATGATGCTGCTTTGAGTATGTTGGACAAAAAATTGCTTGGAAATTTCATGGAGAAAACTCCCTCAAAGGTACATGTTGCTGCAAGAGGCCCAACGAAATATCTCGCTCTACACTTGAGATTTGAGATTGACATGGTGGCCTACTCCCTGTGTGAATTCGGAGGTGGGGAAAGTGAGAAAAAGGAACTCCAAGCCTACAGGGAAATCCATTTTCCGCTGCTCATTAAGCGCTTGAAGAACTCAAAGTATTTATTTTCCATTGCCTTTTTAGTTGTTATTTTCTCTAATTTCGTGGCAGTTTCTCCAAGACAGTTGAGAATGTTGGGGAGGTGTCCATTGGCACCAGAAGAAGCCGGACTTGTTCTTGCTGGTTTAGGTTTTAAGCATGTAACTTACATTTATCTAGCTGGTTCTCAGATATACGGTGGAAGCTCTCGGATGAAATCCTTCACTAGCCTCTACCCTAACTTGGTCACCAAGGAAACTCTCCTCACACCGAGCGAACTTGCACCTTTTCAAAATTTCTCTTCTAAGTTAGCAGCATTGGACTTCATTGCCTGTTCAACAGCCAATGTGTTTGCTATGACTGACTCTGGGAGCCAACTATCCTCCCTAGTGTCTGGATTTCGAACCTACTATGGTGCTGGCGGTGCTCCTACTTTGCGGCCAAACAAGAAGAGGCTAGCAGCAATTTTGTCTGAGAATAGTACTATAGGGTGGAACAGCTTTGAAGACAGAATAAAAAAGATGATTGACGAAGGCCAAAGGGTGCATGTGAGGAGGTTCGGTCGAAGCATTTATCGGCAGCCAAGATGCCCGCAGTGCATGTGCAAATCGCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

602

Amino Acids

67.89

Weight (kDa)

9.37

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 179 - 534 2.3e-65 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 206
AccB1I GGYRCC 2 cut(s) 206, 1303
AccII CGCG 1 cut(s) 1801
AciI CCGC 6 cut(s) 495, 689, 1178, 1611, 1627, 1781
AclWI GGATC 2 cut(s) 77, 845
AcoI YGGCCR 1 cut(s) 1628
AcsI RAATTY 6 cut(s) 130, 352, 696, 1006, 1118, 1483
AcuI CTGAAG 3 cut(s) 332, 599, 854
AfaI GTAC 9 cut(s) 208, 432, 501, 576, 657, 712, 786, 1035, 1672
AfeI AGCGCT 1 cut(s) 1193
AfiI CCNNNNNNNGG 2 cut(s) 216, 1432
AflII CTTAAG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 1036
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 8 cut(s) 222, 448, 510, 651, 868, 1370, 1395, 1689
AluI AGCT 8 cut(s) 222, 448, 510, 651, 868, 1370, 1395, 1689
Alw21I GWGCWC 1 cut(s) 1618
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 2 cut(s) 77, 845
Ama87I CYCGRG 1 cut(s) 35
Aor51HI AGCGCT 1 cut(s) 1193
AoxI GGCC 4 cut(s) 1051, 1101, 1628, 1723
ApeKI GCWGC 8 cut(s) 458, 691, 971, 1043, 1180, 1502, 1649, 1768
ApoI RAATTY 6 cut(s) 130, 352, 696, 1006, 1118, 1483
Asp700I GAANNNNTTC 2 cut(s) 190, 1409
Asp718I GGTACC 1 cut(s) 206
AspLEI GCGC 2 cut(s) 1194, 1803
AspS9I GGNCC 1 cut(s) 1052
AsuHPI GGTGA 2 cut(s) 417, 1429
AsuII TTCGAA 2 cut(s) 250, 1591
AsuNHI GCTAGC 1 cut(s) 1645
AvaI CYCGRG 1 cut(s) 35
BaeGI GKGCMC 1 cut(s) 918
BanI GGYRCC 2 cut(s) 206, 1303
BarI GAAGNNNNNNTAC 2 cut(s) 777, 809
BbsI GAAGAC 1 cut(s) 1701
Bbv12I GWGCWC 1 cut(s) 1618
BbvI GCAGC 8 cut(s) 470, 703, 958, 1030, 1167, 1514, 1661, 1780
BccI CCATC 2 cut(s) 283, 334
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 7 cut(s) 320, 629, 842, 1367, 1416, 1577, 1646
BfmI CTRYAG 2 cut(s) 1159, 1674
BfoI RGCGCY 1 cut(s) 1195
BfrI CTTAAG 1 cut(s) 386
BglII AGATCT 1 cut(s) 742
BmcAI AGTACT 1 cut(s) 1672
BmeT110I CYCGRG 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 1052
BmiI GGNNCC 3 cut(s) 208, 1305, 1562
BmsI GCATC 6 cut(s) 391, 457, 727, 872, 958, 1766
BmtI GCTAGC 1 cut(s) 1649
BpiI GAAGAC 1 cut(s) 1701
Bpu14I TTCGAA 2 cut(s) 250, 1591
BpuEI CTTGAG 1 cut(s) 1099
Bsa29I ATCGAT 1 cut(s) 262
BsaBI GATNNNNATC 1 cut(s) 741
BsaI GGTCTC 1 cut(s) 21
BsaJI CCNNGG 2 cut(s) 823, 1440
BsaXI ACNNNNNCTCC 2 cut(s) 1735, 1765
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 1432
Bse1I ACTGG 1 cut(s) 428
Bse3DI GCAATG 3 cut(s) 471, 1221, 1515
Bse8I GATNNNNATC 1 cut(s) 741
BseCI ATCGAT 1 cut(s) 262
BseDI CCNNGG 2 cut(s) 823, 1440
BseGI GGATG 5 cut(s) 31, 406, 589, 661, 1407
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 2 cut(s) 216, 1432
BseMI GCAATG 3 cut(s) 471, 1221, 1515
BseMII CTCAG 4 cut(s) 525, 855, 1391, 1653
BseNI ACTGG 1 cut(s) 428
BseRI GAGGAG 4 cut(s) 115, 129, 1442, 1756
BseSI GKGCMC 1 cut(s) 918
BseXI GCAGC 8 cut(s) 470, 703, 958, 1030, 1167, 1514, 1661, 1780
Bsh1236I CGCG 1 cut(s) 1801
Bsh1285I CGRYCG 1 cut(s) 1753
BshFI GGCC 4 cut(s) 1053, 1103, 1630, 1725
BshNI GGYRCC 2 cut(s) 206, 1303
BshVI ATCGAT 1 cut(s) 262
BsiEI CGRYCG 1 cut(s) 1753
BsiHKAI GWGCWC 1 cut(s) 1618
BsiHKCI CYCGRG 1 cut(s) 35
BsiSI CCGG 1 cut(s) 1317
BslFI GGGAC 1 cut(s) 82
BslI CCNNNNNNNGG 2 cut(s) 216, 1432
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 82
BsnI GGCC 4 cut(s) 1053, 1103, 1630, 1725
Bso31I GGTCTC 1 cut(s) 21
BsoBI CYCGRG 1 cut(s) 35
Bsp119I TTCGAA 2 cut(s) 250, 1591
Bsp1286I GDGCHC 2 cut(s) 918, 1618
Bsp143I GATC 5 cut(s) 82, 259, 263, 742, 850
BspACI CCGC 6 cut(s) 495, 689, 1178, 1611, 1627, 1781
BspANI GGCC 4 cut(s) 1053, 1103, 1630, 1725
BspCNI CTCAG 4 cut(s) 524, 856, 1390, 1654
BspDI ATCGAT 1 cut(s) 262
BspFNI CGCG 1 cut(s) 1801
BspLI GGNNCC 3 cut(s) 208, 1305, 1562
BspOI GCTAGC 1 cut(s) 1649
BspPI GGATC 2 cut(s) 77, 845
BspT104I TTCGAA 2 cut(s) 250, 1591
BspT107I GGYRCC 2 cut(s) 206, 1303
BspTI CTTAAG 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 21
BsrDI GCAATG 3 cut(s) 471, 1221, 1515
BsrI ACTGG 1 cut(s) 428
BssECI CCNNGG 2 cut(s) 823, 1440
BssMI GATC 5 cut(s) 82, 259, 263, 742, 850
BssT1I CCWWGG 1 cut(s) 1440
Bst4CI ACNGT 3 cut(s) 561, 1275, 1388
Bst6I CTCTTC 4 cut(s) 727, 798, 1495, 1634
BstAFI CTTAAG 1 cut(s) 386
BstBI TTCGAA 2 cut(s) 250, 1591
BstC8I GCNNGC 3 cut(s) 1609, 1647, 1781
BstDEI CTNAG 5 cut(s) 511, 864, 1377, 1494, 1662
BstEII GGTNACC 1 cut(s) 1435
BstF5I GGATG 5 cut(s) 31, 406, 589, 661, 1407
BstFNI CGCG 1 cut(s) 1801
BstH2I RGCGCY 1 cut(s) 1195
BstHHI GCGC 2 cut(s) 1194, 1803
BstKTI GATC 5 cut(s) 85, 262, 266, 745, 853
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 5 cut(s) 82, 259, 263, 742, 850
BstMCI CGRYCG 1 cut(s) 1753
BstMWI GCNNNNNNNGC 7 cut(s) 244, 467, 865, 1189, 1613, 1785, 1800
BstNSI RCATGY 4 cut(s) 1040, 1352, 1739, 1792
BstPI GGTNACC 1 cut(s) 1435
BstSFI CTRYAG 2 cut(s) 1159, 1674
BstSLI GKGCMC 1 cut(s) 918
BstUI CGCG 1 cut(s) 1801
BstV1I GCAGC 8 cut(s) 470, 703, 958, 1030, 1167, 1514, 1661, 1780
BstV2I GAAGAC 1 cut(s) 1701
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
Bsu15I ATCGAT 1 cut(s) 262
BsuRI GGCC 4 cut(s) 1053, 1103, 1630, 1725
BsuTUI ATCGAT 1 cut(s) 262
BtsCI GGATG 5 cut(s) 31, 406, 589, 661, 1407
BtsI GCAGTG 1 cut(s) 1790
BtsIMutI CAGTG 2 cut(s) 483, 1790
Cac8I GCNNGC 3 cut(s) 1609, 1647, 1781
CfoI GCGC 2 cut(s) 1194, 1803
Cfr13I GGNCC 1 cut(s) 1052
ClaI ATCGAT 1 cut(s) 262
Csp6I GTAC 9 cut(s) 207, 431, 500, 575, 656, 711, 785, 1034, 1671
CviAII CATG 9 cut(s) 152, 522, 899, 1012, 1037, 1096, 1349, 1736, 1789
CviQI GTAC 9 cut(s) 207, 431, 500, 575, 656, 711, 785, 1034, 1671
DdeI CTNAG 5 cut(s) 511, 864, 1377, 1494, 1662
DpnI GATC 5 cut(s) 84, 261, 265, 744, 852
DpnII GATC 5 cut(s) 82, 259, 263, 742, 850
EaeI YGGCCR 1 cut(s) 1628
Eam1104I CTCTTC 4 cut(s) 727, 798, 1495, 1634
EarI CTCTTC 4 cut(s) 727, 798, 1495, 1634
Eco130I CCWWGG 1 cut(s) 1440
Eco31I GGTCTC 1 cut(s) 21
Eco47III AGCGCT 1 cut(s) 1193
Eco57I CTGAAG 3 cut(s) 332, 599, 854
Eco88I CYCGRG 1 cut(s) 35
Eco91I GGTNACC 1 cut(s) 1435
EcoO65I GGTNACC 1 cut(s) 1435
EcoRI GAATTC 3 cut(s) 130, 352, 1118
EcoT14I CCWWGG 1 cut(s) 1440
ErhI CCWWGG 1 cut(s) 1440
FaeI CATG 9 cut(s) 155, 525, 902, 1015, 1040, 1099, 1352, 1739, 1792
FalI AAGNNNNNCTT 2 cut(s) 1305, 1337
FaqI GGGAC 1 cut(s) 82
FatI CATG 9 cut(s) 151, 521, 898, 1011, 1036, 1095, 1348, 1735, 1788
FauI CCCGC 1 cut(s) 1788
FauNDI CATATG 1 cut(s) 435
FokI GGATG 5 cut(s) 18, 413, 596, 648, 1414
FspBI CTAG 7 cut(s) 320, 629, 842, 1367, 1416, 1577, 1646
GlaI GCGC 2 cut(s) 1193, 1802
HaeII RGCGCY 1 cut(s) 1195
HaeIII GGCC 4 cut(s) 1053, 1103, 1630, 1725
HapII CCGG 1 cut(s) 1317
HhaI GCGC 2 cut(s) 1194, 1803
Hin1II CATG 9 cut(s) 155, 525, 902, 1015, 1040, 1099, 1352, 1739, 1792
Hin6I GCGC 2 cut(s) 1192, 1801
HinP1I GCGC 2 cut(s) 1192, 1801
HinfI GANTC 3 cut(s) 191, 311, 1553
HpaII CCGG 1 cut(s) 1317
HphI GGTGA 2 cut(s) 417, 1429
Hpy166II GTNNAC 4 cut(s) 29, 483, 592, 817
Hpy188III TCNNGA 3 cut(s) 35, 111, 1584
Hpy8I GTNNAC 4 cut(s) 29, 483, 592, 817
HpyCH4III ACNGT 3 cut(s) 561, 1275, 1388
HpyCH4IV ACGT 2 cut(s) 633, 658
HpyCH4V TGCA 8 cut(s) 476, 740, 885, 1046, 1472, 1735, 1788, 1794
HpyF10VI GCNNNNNNNGC 7 cut(s) 244, 467, 865, 1189, 1613, 1785, 1800
HpyF3I CTNAG 5 cut(s) 511, 864, 1377, 1494, 1662
HpySE526I ACGT 2 cut(s) 633, 658
Hsp92II CATG 9 cut(s) 155, 525, 902, 1015, 1040, 1099, 1352, 1739, 1792
HspAI GCGC 2 cut(s) 1192, 1801
KpnI GGTACC 1 cut(s) 210
Kzo9I GATC 5 cut(s) 82, 259, 263, 742, 850
LmnI GCTCC 3 cut(s) 219, 1560, 1621
Lsp1109I GCAGC 8 cut(s) 470, 703, 958, 1030, 1167, 1514, 1661, 1780
LweI GCATC 6 cut(s) 391, 457, 727, 872, 958, 1766
MaeI CTAG 7 cut(s) 320, 629, 842, 1367, 1416, 1577, 1646
MaeII ACGT 2 cut(s) 633, 658
MaeIII GTNAC 4 cut(s) 675, 890, 1351, 1435
MalI GATC 5 cut(s) 84, 261, 265, 744, 852
MboI GATC 5 cut(s) 82, 259, 263, 742, 850
MboII GAAGA 9 cut(s) 338, 554, 714, 785, 1210, 1322, 1482, 1651, 1706
MfeI CAATTG 2 cut(s) 642, 765
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 2 cut(s) 918, 1618
MlyI GAGTC 1 cut(s) 1547
MmeI TCCRAC 2 cut(s) 949, 966
MroXI GAANNNNTTC 2 cut(s) 190, 1409
MseI TTAA 4 cut(s) 387, 878, 1188, 1344
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 1180
MspCI CTTAAG 1 cut(s) 386
MspI CCGG 1 cut(s) 1317
MunI CAATTG 2 cut(s) 642, 765
MvnI CGCG 1 cut(s) 1801
MwoI GCNNNNNNNGC 7 cut(s) 244, 467, 865, 1189, 1613, 1785, 1800
NdeI CATATG 1 cut(s) 435
NdeII GATC 5 cut(s) 82, 259, 263, 742, 850
NheI GCTAGC 1 cut(s) 1645
NlaIII CATG 9 cut(s) 155, 525, 902, 1015, 1040, 1099, 1352, 1739, 1792
NlaIV GGNNCC 3 cut(s) 208, 1305, 1562
NmuCI GTSAC 1 cut(s) 1435
NspI RCATGY 4 cut(s) 1040, 1352, 1739, 1792
NspV TTCGAA 2 cut(s) 250, 1591
PciI ACATGT 1 cut(s) 1036
PdmI GAANNNNTTC 2 cut(s) 190, 1409
PfeI GAWTC 2 cut(s) 191, 311
PleI GAGTC 1 cut(s) 1547
PpsI GAGTC 1 cut(s) 1547
PscI ACATGT 1 cut(s) 1036
PspEI GGTNACC 1 cut(s) 1435
PspN4I GGNNCC 3 cut(s) 208, 1305, 1562
PspPI GGNCC 1 cut(s) 1052
PsuI RGATCY 1 cut(s) 742
RsaI GTAC 9 cut(s) 208, 432, 501, 576, 657, 712, 786, 1035, 1672
RsaNI GTAC 9 cut(s) 207, 431, 500, 575, 656, 711, 785, 1034, 1671
RseI CAYNNNNRTG 1 cut(s) 438
SaqAI TTAA 4 cut(s) 387, 878, 1188, 1344
Sau3AI GATC 5 cut(s) 82, 259, 263, 742, 850
Sau96I GGNCC 1 cut(s) 1052
ScaI AGTACT 1 cut(s) 1672
SchI GAGTC 1 cut(s) 1547
SduI GDGCHC 2 cut(s) 918, 1618
SfaNI GCATC 6 cut(s) 391, 457, 727, 872, 958, 1766
SfcI CTRYAG 2 cut(s) 1159, 1674
SfuI TTCGAA 2 cut(s) 250, 1591
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 2 cut(s) 386, 1078
SmoI CTYRAG 2 cut(s) 386, 1078
SsiI CCGC 6 cut(s) 495, 689, 1178, 1611, 1627, 1781
SspMI CTAG 7 cut(s) 320, 629, 842, 1367, 1416, 1577, 1646
StyI CCWWGG 1 cut(s) 1440
TaaI ACNGT 3 cut(s) 561, 1275, 1388
TaiI ACGT 2 cut(s) 636, 661
TaqI TCGA 6 cut(s) 250, 262, 338, 838, 1591, 1753
TaqII GACCGA 1 cut(s) 1739
TatI WGTACW 4 cut(s) 430, 574, 784, 1670
TauI GCSGC 2 cut(s) 498, 1630
TfiI GAWTC 2 cut(s) 191, 311
Tru1I TTAA 4 cut(s) 387, 878, 1188, 1344
Tru9I TTAA 4 cut(s) 387, 878, 1188, 1344
TscAI CASTG 2 cut(s) 490, 1790
TseFI GTSAC 1 cut(s) 1435
TseI GCWGC 8 cut(s) 458, 691, 971, 1043, 1180, 1502, 1649, 1768
Tsp45I GTSAC 1 cut(s) 1435
TspDTI ATGAA 9 cut(s) 140, 164, 510, 600, 672, 887, 1000, 1418, 1504
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 2 cut(s) 490, 1790
Vha464I CTTAAG 1 cut(s) 386
XapI RAATTY 6 cut(s) 130, 352, 696, 1006, 1118, 1483
XceI RCATGY 4 cut(s) 1040, 1352, 1739, 1792
XmnI GAANNNNTTC 2 cut(s) 190, 1409
XspI CTAG 7 cut(s) 320, 629, 842, 1367, 1416, 1577, 1646
ZrmI AGTACT 1 cut(s) 1672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.