Rroxscaffold_1G00065150

protein At1g04910-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006470
Physical Location & Seq
Forward (+)
86913348 .. 86922100
8753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065150.1

Sequence Viewer

Length: 4602 bp
ATGGAGAGAGCTCAGTTCTTCCTCTTGTGCATAACATTGTTGCTTTGCATGGCCAGAGCAAAAACCAATTTGACCACAGACCAGTCTGCTCTTCTTGCTCTCAAAGCTAATGTCACTAGTGACCCTCAAAACTTGTTGTCCAACTGGTCTACCAATACCTCCGTTTGCAACTGGGTTGGGGTTACTTGTGGTCATGTCCATCTTAGAGTCGCTTCCTTGAATCTCTCTTATTTTGGCCTCACAGGTAGAATTCCTCCGGAGCTAGGAAACCTTTCATTTCTTGTTGTGCTACGCTTGAGAAACAACAGTTTTAATGGCACTTTGCCAGTGGAATTAGCTGGTTTGCGCCGATTGAAGTTGGTTAGCTTCAAATACAATAACTTTATGGGGGAGATTCCATCATGGTTTGGGTCCTTATCCAAACTTAAAGCCTTGATTTTGTATGGGAATCGGTTCACAGGATCCATACCTGCTGCTATCTTCAACTTATCTGCTCTGCAAGTATTTGATCTGAGCAATAACCAGCTATCAGGTAGCATACCGAGAGAAATCGGGAACTTAACAATGCTGAAGCATTTATACCTTGACAATAACAATTTCCGAGAAATTCCGGATGAGATCGGCAGTTTACATCAGCTGGATTTGCTGTCTATGCAATACAATGGCCTAAAAGGGCGTGTTCCTTTGGTTTTCTTCAACATGTCTTCTTTGACTATTTTGGGCCTTACAGGAAACAGTTTCAGTGGTCGTCTTCCTGACAATATATGTCAGAATCTTCCTAGTATTCAAGGGTTGTACTTTTCTATCAACCAGTTTGATGGTCCACTTCCATCCCAATTATGGCAGTGCAAACAGCTTCTTGTATTGTCATTATCTGTTAACAATTTCAGTGGAAGTATACCCAGAAATATTGGCAACTTGACCCAGTTACAGAAGATTTATCTAGGCTTCAACAATTTGACAGGTACTATTCCACATGAGATTGGCTATCTTCAAAATTTAGAGATATTGTCACTCCTTGCTAACTCATTTTCTGGGTCCATTCCTACCACACTCTGTGCCTTACCAGAACTCCTGTCACTTTACTTGGGCAAGAATAAGTTAACCATTGATACTTCTACTCCAGAAGCCAACATTTTCTCTTGTTTGGCCAATCTTAAAAATTTGTGGATATTAAATTTGTCAAGCAATCCATTATATGCCACGCTTCCTGTTTCCCTCAAGAACTTCTCTACATCCATTCAATATCTGGATATGAGCAGTTGCAACATAGGGGGTAACATTACCAACGGTATTGGCAACTTGAGCAACTTGACAGTCTTAGACTTGGGATACAATCAACTGAGTGGATCAATTCCAACTACAATGGGAAGACTACAGAACCTCCAGGGTTTGTACTTGACTGATAACAAATTGCAAGGATTTATTCCGGATGAACTTTGTCAACTAAACAATGTAGTTGACTTGGTTTTGGGTGGTAATCAACTTTCTGGTTCTGTACCTTCCTGCTTGGGTAATCTAACAGCTCTTAGAACTCTGTCATTAGGGTCCAATTCTTTAACTTCAATGGTACCATCTGCAGTGTGGGGACTTACATATATCTTACACATAAACTTGTCATCCAATTCTCTACTTGGTCCTATCTCAGTAGATGTTGGAAATTTAAGAGTTGTCACAAATATAGATTTGTCAAGCAACAACTTTTCAGGTACTATACCAAGCAACATTGGGTTGCTTGAAAACTTGGTTAATCTCTCCTTGGCAAATAACAATCTCGAAGGCCCAATTCCCAGTTCATTTGGCAACTTAGTAAGCCTGGAGCTCTTGGATCTATCCAGAAACAATCTATCTGGAATCATTCCAGAGTCTTTAGAAGCGCTCTTGCATCTCAGGTATCTGAATTTGTCTTTCAACAAACTCCATGGCGAAATTCCAACAGGTGGACCTTTCAAAAACTTCTCTGATCAGTCATTTCTCTCAAATGATGGACTATGTGGTGCATCCCAGCTTGATTTTCTACCGTGCAGAACCCCTGTACAATATTCAAGGACAGCTAGTTCATCTATGTTGAAATATATTATTCCAGGGATGTTGGCAGCAATGCTTGTATTGACCTTCATATGGTTGTTCACACTACGCAAAAAAGGGGATGCAGAGGTTGCAACAGAGCACACCTTAATACCTGAACTTCTTCGGAAAAAAGTTACATACCAAGAAATTCTAAGTGCTACAAATGGCTTCAATGAAACCAACTTACTTGGTTCGGGGGGATTTGGCTCAGTGTATAAAGGAATACTTTCAAATGGGATAGCGGTTGCTATAAAGGTGTTCAATTTACAGCACGAAGAGGCTTTCAAAAAATTTGATGTTGAAAGTGAACTGCTAAGCAATGTTTGTCATCGCAATCTTATCAAAATCATCGGTAGTTGCAACCAAAGTCATTTCAAAGCCTTGGTACTTGACTACATGCCTAATGGGAGCCTCGAGAGGTTGCTGTATTCTGAAGAATACTGTTCTTTGAAAATCCTAGAGAGGTTGAACATAATGATAGATGTTGCATCGGCGTTGGAATACCTTCATCATGGTTATTCAATACCAATTGTCCATTGTGATTTGAAGCCAAGCAATATACTACTAGACAATGATATGGTTGCGCATGTTGCTGATTTTGGCATTGCAAAACTCTCAGAATATGGAATAGACGGAATAGTTACCAGAAGAGGGGATGTGTACAGTTTTGGTATAGTACTAATGGAGACTTTCACAAAAAAGAAGCCTACAGATGAGATATTTGTTGGGGAAATGAGTTTAAAGCGATGGGTTGCAGATTCGTTACTTTCAGATACAGTACTTGAAGTTGTGGATGGCAGTTTACTTGGGAAAGAGGAAGATCATGATTTTGTGAATAAGAGGGATTGTTTATCTTCCATTATGAGGTTGGCACTAGATTGTTCTGCAGAATCAGTTAAAGAGAGGATAAGTATGCAAGATGCTGCAGTCACACTCCATAAAATCAAGAACAATTTTTTGAAGGACATTGGAGGAGTTGAGTTGAACAGTTCAACTTTAAGAAAATCTCTGGGGCTTAAGGAGGACAAAATGGATGCCTACATTATGAAAGAAAAATCACCAGTTCAGATGTATGATCGCCTACTAAAGTTGGCGTCTGCATCCCTTGCAAAGAAAGAGTTCAAGCAAGAGTCATCAACCTTATGGGAAGAACCAAATAGGAGGCAAGTTGCTGCATGGAAACCTTGTGCGGATAGAAAAGTTTCGAAAAGCCTAGGAAAATATAAGAAAAGTAATGGCTACATAATAGTCAGTGCAAATGGTGGTCTCAATCAACAACGAGTTGCTATTTGCAATGCTGTTGCTGTGGCATCTCTTCTAAATGCTACTCTTGTTCTTCCAAGATTTCTTTACAGCAATGTGTGGAATGATCCGAGTCAATTTGGTGATATTTACCAAGAAGAATATTTTATAGATGTAATGAAGGATGAAGTAAACTTTGTCAAGGAACTCCCTTCTCATTTGGCATCACTAGACCTTGAAGCAATTGGTAGCCTTATTACTGATGCAGATCTTGTGAAGGAGGCAAAACCAACTGATTATGTTAGAACTGTGCTTCCTATTCTCTTGAAAAATGGAGTTGTTCACTTCCTGGGATATGGGAATCGACTAGGCTTCGATCCATTGCCTTCTGAGCTTCAGAGACTAAGATGCAAATGCAACTTCCATGCTTTGAAATTCGTGCCCGAAATCCAACGAGTTGGTTCTTTATTGGTTACAAGGATAAGAAAATATGATGCTGCACTGAGTATGTTGGACAAACAATTGCTTGGAAACTTCATGCACAGCATTCACTCAAAACAGCATGATGCCGCTGCAAGAGGCCCGTTCAAATATCTTGCTCTACACTTGAGATTCGAGATTGACATGGTAGCCTACTCCCTCTGTGACTTTGGAGGTGGAGAAAGTGAGAGAAATGAACTTCAAGCATACAGAGAAATCCATTTTCCTCTGCTCATCGAGCGCTTAAAGAACTCGAAGTCTGTTTCTCAAAGTGAGTTGAGAAAGTTGGGGAAATGTCCATTGACACCGGAAGAAGCAGCACTAGTTCTTGCTGGTATAGGTTTTAAGCATAGAACTTACATTTACCTAGCTGGATCTCAGATATATGGTGGAAACTCCAGGATGAATTCCTTCACCAACCTCTATCCTAATTTGGTCACAAAAGAAACTCTCCTCACACCAAGAGAACTTGCACCTTTTCAAAACTTCTCTTCCAAGTTAGCAGCACTAGACTTCATTGCCTGTGCAACTGCCGATGTGTTTGCTATGACTGACTCCGGGAGCCAACTATCCTCCCTGGTATCTGGATTTCGAACCTATTATGGTGGCGGCCATGCTCCTACTCTGCGGCCTAACAAGAAGAGGCTAGCAGCAATTTTGTCTGAGAATAGTACCATAGGCTGGAATAGTTTTGAAGACAGGGTTAAAAAGATGATCGAGGAAGGTCAAAGGGTACATGTAAGGAGGTTTGGTCGAAGCATTTATCGACAGCCAAGATGCCCAGAGTGCATGTGTAAATCCTACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

1533

Amino Acids

169.63

Weight (kDa)

8.41

Isoelectric Point (pI)

34.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 63 1e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 67 - 147 9e-06 Leucine-rich repeat region
LRR_8 PF13855 133 - 176 7.3e-06 Leucine rich repeat
LRR_8 PF13855 141 - 200 3.8e-09 Leucine rich repeat
LRR_14 PF23598 162 - 265 8.4e-11 Leucine-rich repeat region
LRR_14 PF23598 278 - 396 2.5e-09 Leucine-rich repeat region
LRR_14 PF23598 407 - 518 1.6e-09 Leucine-rich repeat region
LRR_8 PF13855 436 - 496 6.4e-09 Leucine rich repeat
LRR_8 PF13855 489 - 544 3e-06 Leucine rich repeat
LRR_14 PF23598 546 - 640 1.4e-07 Leucine-rich repeat region
LRR_8 PF13855 581 - 640 4.1e-10 Leucine rich repeat
Pkinase PF00069 748 - 927 1.5e-29 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 751 - 997 1.4e-28 Protein tyrosine and serine/threonine kinase
O-FucT PF10250 1104 - 1465 9e-92 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2655
Acc36I ACCTGC 1 cut(s) 478
Acc65I GGTACC 1 cut(s) 1570
AccB1I GGYRCC 1 cut(s) 1570
AccB7I CCANNNNNTGG 2 cut(s) 1940, 4473
AccI GTMKAC 2 cut(s) 149, 898
AccIII TCCGGA 3 cut(s) 256, 610, 1429
AciI CCGC 5 cut(s) 2312, 3260, 3882, 4401, 4420
AclWI GGATC 7 cut(s) 456, 469, 1357, 1836, 3434, 3683, 4174
AcoI YGGCCR 3 cut(s) 51, 1149, 4402
AcuI CTGAAG 3 cut(s) 590, 2523, 3692
AcyI GRCGYC 1 cut(s) 3164
AdeI CACNNNGTG 1 cut(s) 1058
AfeI AGCGCT 2 cut(s) 1878, 4034
AfiI CCNNNNNNNGG 7 cut(s) 263, 840, 1940, 2121, 2721, 2934, 4473
AflII CTTAAG 1 cut(s) 3086
AflIII ACRYGT 2 cut(s) 699, 4528
AhlI ACTAGT 2 cut(s) 116, 4114
AjnI CCWGG 6 cut(s) 1386, 1815, 2083, 3660, 4190, 4368
AjuI GAANNNNNNNTTGG 2 cut(s) 1777, 1809
AloI GAACNNNNNNTCC 4 cut(s) 2041, 2073, 3610, 3642
Alw21I GWGCWC 3 cut(s) 13, 1824, 2172
Alw26I GTCTC 3 cut(s) 2750, 3341, 3706
AlwI GGATC 7 cut(s) 456, 469, 1357, 1836, 3434, 3683, 4174
Ama87I CYCGRG 1 cut(s) 2483
Aor13HI TCCGGA 3 cut(s) 256, 610, 1429
Aor51HI AGCGCT 2 cut(s) 1878, 4034
AoxI GGCC 9 cut(s) 51, 235, 664, 721, 1149, 1780, 3892, 4402, 4421
ApeKI GCWGC 9 cut(s) 473, 2096, 2993, 3242, 3809, 3884, 4109, 4295, 4442
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
Asp700I GAANNNNTTC 8 cut(s) 271, 452, 2190, 2296, 2574, 3188, 3271, 4202
Asp718I GGTACC 1 cut(s) 1570
AspA2I CCTAGG 1 cut(s) 3283
AspLEI GCGC 4 cut(s) 348, 1879, 2656, 4035
AspS9I GGNCC 9 cut(s) 411, 721, 821, 1038, 1548, 1637, 1781, 1943, 3893
AsuC2I CCSGG 1 cut(s) 4351
AsuHPI GGTGA 3 cut(s) 3120, 3467, 4198
AsuII TTCGAA 2 cut(s) 3275, 4384
AsuNHI GCTAGC 1 cut(s) 4438
AvaI CYCGRG 1 cut(s) 2483
AvaII GGWCC 6 cut(s) 411, 821, 1038, 1548, 1637, 1943
AvrII CCTAGG 1 cut(s) 3283
BaeGI GKGCMC 1 cut(s) 3756
BalI TGGCCA 2 cut(s) 53, 1151
BamHI GGATCC 1 cut(s) 461
BanI GGYRCC 1 cut(s) 1570
BanII GRGCYC 2 cut(s) 13, 1824
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BbsI GAAGAC 4 cut(s) 696, 743, 1378, 4494
Bbv12I GWGCWC 3 cut(s) 13, 1824, 2172
BbvI GCAGC 9 cut(s) 460, 2108, 2980, 3229, 3796, 3871, 4121, 4307, 4454
BccI CCATC 8 cut(s) 207, 406, 812, 838, 1582, 1979, 2811, 2858
BcgI CGANNNNNNTGC 2 cut(s) 4316, 4350
BciT130I CCWGG 6 cut(s) 1388, 1817, 2085, 3662, 4192, 4370
BciVI GTATCC 1 cut(s) 1325
BclI TGATCA 1 cut(s) 1963
BcnI CCSGG 1 cut(s) 4351
BcoDI GTCTC 3 cut(s) 2750, 3341, 3706
BcuI ACTAGT 2 cut(s) 116, 4114
BfmI CTRYAG 5 cut(s) 1376, 1578, 2778, 2955, 2994
BfoI RGCGCY 2 cut(s) 1880, 4036
BfrI CTTAAG 1 cut(s) 3086
BfuAI ACCTGC 1 cut(s) 478
BfuI GTATCC 1 cut(s) 1325
BglII AGATCT 1 cut(s) 3580
BlnI CCTAGG 1 cut(s) 3283
BlpI GCTNAGC 1 cut(s) 2384
BmcAI AGTACT 2 cut(s) 2748, 2850
Bme1390I CCNGG 7 cut(s) 1388, 1817, 2085, 3662, 4192, 4351, 4370
Bme18I GGWCC 6 cut(s) 411, 821, 1038, 1548, 1637, 1943
BmeT110I CYCGRG 1 cut(s) 2483
BmgT120I GGNCC 9 cut(s) 411, 721, 821, 1038, 1548, 1637, 1781, 1943, 3893
BmiI GGNNCC 7 cut(s) 412, 463, 1039, 1549, 1572, 2480, 4355
BmrFI CCNGG 7 cut(s) 1388, 1817, 2085, 3662, 4192, 4351, 4370
BmrI ACTGGG 3 cut(s) 181, 919, 1785
BmtI GCTAGC 1 cut(s) 4442
BmuI ACTGGG 3 cut(s) 181, 919, 1785
BpiI GAAGAC 4 cut(s) 696, 743, 1378, 4494
BpmI CTGGAG 4 cut(s) 1107, 1370, 1838, 4174
Bpu1102I GCTNAGC 1 cut(s) 2384
Bpu14I TTCGAA 2 cut(s) 3275, 4384
BpuEI CTTGAG 4 cut(s) 316, 1205, 1324, 3940
BpuMI CCSGG 1 cut(s) 4351
BsaBI GATNNNNATC 1 cut(s) 3579
BsaHI GRCGYC 1 cut(s) 3164
BsaI GGTCTC 1 cut(s) 3341
BsaJI CCNNGG 8 cut(s) 1387, 1758, 1921, 2084, 2451, 3283, 3661, 4368
BsaWI WCCGGW 4 cut(s) 256, 610, 1429, 4099
BsaXI ACNNNNNCTCC 8 cut(s) 1056, 1086, 1105, 1135, 1368, 1398, 4528, 4558
Bsc4I CCNNNNNNNGG 7 cut(s) 263, 840, 1940, 2121, 2721, 2934, 4473
Bse1I ACTGG 8 cut(s) 82, 149, 176, 326, 811, 925, 1791, 3131
Bse3DI GCAATG 7 cut(s) 2106, 2395, 2673, 3370, 3433, 3692, 4308
Bse8I GATNNNNATC 1 cut(s) 3579
BseAI TCCGGA 3 cut(s) 256, 610, 1429
BseBI CCWGG 6 cut(s) 1388, 1817, 2085, 3662, 4192, 4370
BseDI CCNNGG 8 cut(s) 1387, 1758, 1921, 2084, 2451, 3283, 3661, 4368
BseJI GATNNNNATC 1 cut(s) 3579
BseLI CCNNNNNNNGG 7 cut(s) 263, 840, 1940, 2121, 2721, 2934, 4473
BseMI GCAATG 7 cut(s) 2106, 2395, 2673, 3370, 3433, 3692, 4308
BseNI ACTGG 8 cut(s) 82, 149, 176, 326, 811, 925, 1791, 3131
BseRI GAGGAG 2 cut(s) 3057, 4235
BseSI GKGCMC 1 cut(s) 3756
BseXI GCAGC 9 cut(s) 460, 2108, 2980, 3229, 3796, 3871, 4121, 4307, 4454
BseYI CCCAGC 1 cut(s) 2004
BsgI GTGCAG 2 cut(s) 2044, 3795
BshFI GGCC 9 cut(s) 53, 237, 666, 723, 1151, 1782, 3894, 4404, 4423
BshNI GGYRCC 1 cut(s) 1570
BsiHKAI GWGCWC 3 cut(s) 13, 1824, 2172
BsiHKCI CYCGRG 1 cut(s) 2483
BsiSI CCGG 5 cut(s) 257, 611, 1430, 4100, 4350
BslFI GGGAC 1 cut(s) 1602
BslI CCNNNNNNNGG 7 cut(s) 263, 840, 1940, 2121, 2721, 2934, 4473
BsmAI GTCTC 3 cut(s) 2750, 3341, 3706
BsmFI GGGAC 1 cut(s) 1602
BsmI GAATGC 1 cut(s) 3858
BsnI GGCC 9 cut(s) 53, 237, 666, 723, 1151, 1782, 3894, 4404, 4423
Bso31I GGTCTC 1 cut(s) 3341
BsoBI CYCGRG 1 cut(s) 2483
Bsp119I TTCGAA 2 cut(s) 3275, 4384
Bsp1286I GDGCHC 4 cut(s) 13, 1824, 2172, 3756
Bsp13I TCCGGA 3 cut(s) 256, 610, 1429
Bsp1407I TGTACA 2 cut(s) 2035, 2730
Bsp1720I GCTNAGC 1 cut(s) 2384
Bsp19I CCATGG 1 cut(s) 1921
BspACI CCGC 5 cut(s) 2312, 3260, 3882, 4401, 4420
BspANI GGCC 9 cut(s) 53, 237, 666, 723, 1151, 1782, 3894, 4404, 4423
BspEI TCCGGA 3 cut(s) 256, 610, 1429
BspHI TCATGA 1 cut(s) 2893
BspLI GGNNCC 7 cut(s) 412, 463, 1039, 1549, 1572, 2480, 4355
BspMAI CTGCAG 3 cut(s) 1582, 2959, 2998
BspMI ACCTGC 1 cut(s) 478
BspOI GCTAGC 1 cut(s) 4442
BspPI GGATC 7 cut(s) 456, 469, 1357, 1836, 3434, 3683, 4174
BspQI GCTCTTC 1 cut(s) 96
BspT104I TTCGAA 2 cut(s) 3275, 4384
BspT107I GGYRCC 1 cut(s) 1570
BspTI CTTAAG 1 cut(s) 3086
BspTNI GGTCTC 1 cut(s) 3341
BsrDI GCAATG 7 cut(s) 2106, 2395, 2673, 3370, 3433, 3692, 4308
BsrGI TGTACA 2 cut(s) 2035, 2730
BsrI ACTGG 8 cut(s) 82, 149, 176, 326, 811, 925, 1791, 3131
BssECI CCNNGG 8 cut(s) 1387, 1758, 1921, 2084, 2451, 3283, 3661, 4368
BssNAI GTATAC 1 cut(s) 899
BssNI GRCGYC 1 cut(s) 3164
BssT1I CCWWGG 4 cut(s) 1758, 1921, 2451, 3283
Bst1107I GTATAC 1 cut(s) 899
Bst2UI CCWGG 6 cut(s) 1388, 1817, 2085, 3662, 4192, 4370
Bst6I CTCTTC 6 cut(s) 96, 2340, 2713, 3390, 4288, 4427
BstACI GRCGYC 1 cut(s) 3164
BstAFI CTTAAG 1 cut(s) 3086
BstAPI GCANNNNNTGC 2 cut(s) 2159, 3176
BstAUI TGTACA 2 cut(s) 2035, 2730
BstBI TTCGAA 2 cut(s) 3275, 4384
BstC8I GCNNGC 1 cut(s) 4440
BstDSI CCRYGG 1 cut(s) 1921
BstH2I RGCGCY 2 cut(s) 1880, 4036
BstHHI GCGC 4 cut(s) 348, 1879, 2656, 4035
BstMAI GTCTC 3 cut(s) 2750, 3341, 3706
BstNI CCWGG 6 cut(s) 1388, 1817, 2085, 3662, 4192, 4370
BstNSI RCATGY 5 cut(s) 703, 2470, 2660, 4532, 4585
BstSCI CCNGG 7 cut(s) 1386, 1815, 2083, 3660, 4190, 4349, 4368
BstSFI CTRYAG 5 cut(s) 1376, 1578, 2778, 2955, 2994
BstSLI GKGCMC 1 cut(s) 3756
BstV1I GCAGC 9 cut(s) 460, 2108, 2980, 3229, 3796, 3871, 4121, 4307, 4454
BstV2I GAAGAC 4 cut(s) 696, 743, 1378, 4494
BstX2I RGATCY 4 cut(s) 461, 1828, 3580, 4166
BstXI CCANNNNNNTGG 2 cut(s) 818, 3770
BstYI RGATCY 4 cut(s) 461, 1828, 3580, 4166
BstZ17I GTATAC 1 cut(s) 899
BsuI GTATCC 1 cut(s) 1325
BsuRI GGCC 9 cut(s) 53, 237, 666, 723, 1151, 1782, 3894, 4404, 4423
BtgI CCRYGG 1 cut(s) 1921
BtgZI GCGATG 2 cut(s) 2384, 2830
BtsI GCAGTG 2 cut(s) 851, 1587
BtsIMutI CAGTG 8 cut(s) 333, 748, 851, 895, 1587, 2286, 3328, 3812
BveI ACCTGC 1 cut(s) 478
Cac8I GCNNGC 1 cut(s) 4440
CciI TCATGA 1 cut(s) 2893
CfoI GCGC 4 cut(s) 348, 1879, 2656, 4035
Cfr13I GGNCC 9 cut(s) 411, 721, 821, 1038, 1548, 1637, 1781, 1943, 3893
CseI GACGC 1 cut(s) 3153
CspCI CAANNNNNGTGG 2 cut(s) 871, 906
DraI TTTAAA 1 cut(s) 2811
DraIII CACNNNGTG 1 cut(s) 1058
EaeI YGGCCR 3 cut(s) 51, 1149, 4402
Eam1104I CTCTTC 6 cut(s) 96, 2340, 2713, 3390, 4288, 4427
EarI CTCTTC 6 cut(s) 96, 2340, 2713, 3390, 4288, 4427
Ecl136II GAGCTC 2 cut(s) 11, 1822
Eco130I CCWWGG 4 cut(s) 1758, 1921, 2451, 3283
Eco24I GRGCYC 2 cut(s) 13, 1824
Eco31I GGTCTC 1 cut(s) 3341
Eco47I GGWCC 6 cut(s) 411, 821, 1038, 1548, 1637, 1943
Eco47III AGCGCT 2 cut(s) 1878, 4034
Eco53kI GAGCTC 2 cut(s) 11, 1822
Eco57I CTGAAG 3 cut(s) 590, 2523, 3692
Eco88I CYCGRG 1 cut(s) 2483
EcoICRI GAGCTC 2 cut(s) 11, 1822
EcoO109I RGGNCCY 1 cut(s) 411
EcoRI GAATTC 2 cut(s) 249, 4198
EcoRII CCWGG 6 cut(s) 1386, 1815, 2083, 3660, 4190, 4368
EcoT14I CCWWGG 4 cut(s) 1758, 1921, 2451, 3283
EcoT38I GRGCYC 2 cut(s) 13, 1824
ErhI CCWWGG 4 cut(s) 1758, 1921, 2451, 3283
FalI AAGNNNNNCTT 2 cut(s) 2280, 2312
FaqI GGGAC 1 cut(s) 1602
FauNDI CATATG 1 cut(s) 2120
FbaI TGATCA 1 cut(s) 1963
FblI GTMKAC 2 cut(s) 149, 898
FriOI GRGCYC 2 cut(s) 13, 1824
FspI TGCGCA 1 cut(s) 2655
GlaI GCGC 4 cut(s) 347, 1878, 2655, 4034
GsaI CCCAGC 1 cut(s) 2008
GsuI CTGGAG 4 cut(s) 1107, 1370, 1838, 4174
HaeII RGCGCY 2 cut(s) 1880, 4036
HaeIII GGCC 9 cut(s) 53, 237, 666, 723, 1151, 1782, 3894, 4404, 4423
HapII CCGG 5 cut(s) 257, 611, 1430, 4100, 4350
HgaI GACGC 1 cut(s) 3153
HhaI GCGC 4 cut(s) 348, 1879, 2656, 4035
Hin1I GRCGYC 1 cut(s) 3164
Hin6I GCGC 4 cut(s) 346, 1877, 2654, 4033
HinP1I GCGC 4 cut(s) 346, 1877, 2654, 4033
HincII GTYRAC 4 cut(s) 880, 1104, 1445, 1462
HindII GTYRAC 4 cut(s) 880, 1104, 1445, 1462
HpaI GTTAAC 2 cut(s) 880, 1104
HpaII CCGG 5 cut(s) 257, 611, 1430, 4100, 4350
HphI GGTGA 3 cut(s) 3120, 3467, 4198
Hsp92I GRCGYC 1 cut(s) 3164
HspAI GCGC 4 cut(s) 346, 1877, 2654, 4033
Kpn2I TCCGGA 3 cut(s) 256, 610, 1429
KpnI GGTACC 1 cut(s) 1574
Ksp22I TGATCA 1 cut(s) 1963
KspAI GTTAAC 2 cut(s) 880, 1104
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 5 cut(s) 259, 1819, 2478, 4353, 4414
Lsp1109I GCAGC 9 cut(s) 460, 2108, 2980, 3229, 3796, 3871, 4121, 4307, 4454
MfeI CAATTG 3 cut(s) 2598, 3555, 3833
MflI RGATCY 4 cut(s) 461, 1828, 3580, 4166
MhlI GDGCHC 4 cut(s) 13, 1824, 2172, 3756
MlsI TGGCCA 2 cut(s) 53, 1151
MluNI TGGCCA 2 cut(s) 53, 1151
MlyI GAGTC 5 cut(s) 216, 1874, 3209, 3454, 4340
MmeI TCCRAC 7 cut(s) 165, 1382, 1636, 1958, 2547, 3787, 3804
Mox20I TGGCCA 2 cut(s) 53, 1151
MroI TCCGGA 3 cut(s) 256, 610, 1429
MroXI GAANNNNTTC 8 cut(s) 271, 452, 2190, 2296, 2574, 3188, 3271, 4202
MscI TGGCCA 2 cut(s) 53, 1151
Msp20I TGGCCA 2 cut(s) 53, 1151
MspA1I CMGCKG 2 cut(s) 637, 3884
MspCI CTTAAG 1 cut(s) 3086
MspI CCGG 5 cut(s) 257, 611, 1430, 4100, 4350
MspR9I CCNGG 7 cut(s) 1388, 1817, 2085, 3662, 4192, 4351, 4370
MunI CAATTG 3 cut(s) 2598, 3555, 3833
Mva1269I GAATGC 1 cut(s) 3858
MvaI CCWGG 6 cut(s) 1388, 1817, 2085, 3662, 4192, 4370
NciI CCSGG 1 cut(s) 4351
NcoI CCATGG 1 cut(s) 1921
NdeI CATATG 1 cut(s) 2120
NheI GCTAGC 1 cut(s) 4438
NlaIV GGNNCC 7 cut(s) 412, 463, 1039, 1549, 1572, 2480, 4355
NmuCI GTSAC 8 cut(s) 112, 119, 1011, 1077, 1672, 2998, 3956, 4228
NsbI TGCGCA 1 cut(s) 2655
NspI RCATGY 5 cut(s) 703, 2470, 2660, 4532, 4585
NspV TTCGAA 2 cut(s) 3275, 4384
PaeR7I CTCGAG 1 cut(s) 2483
PagI TCATGA 1 cut(s) 2893
PciI ACATGT 2 cut(s) 699, 4528
PciSI GCTCTTC 1 cut(s) 96
PctI GAATGC 1 cut(s) 3858
PdmI GAANNNNTTC 8 cut(s) 271, 452, 2190, 2296, 2574, 3188, 3271, 4202
PfeI GAWTC 9 cut(s) 220, 394, 448, 772, 1854, 2828, 2960, 3673, 3924
PflMI CCANNNNNTGG 2 cut(s) 1940, 4473
PfoI TCCNGGA 2 cut(s) 4190, 4349
PleI GAGTC 5 cut(s) 215, 1873, 3208, 3453, 4340
PpsI GAGTC 5 cut(s) 215, 1873, 3208, 3453, 4340
PpuMI RGGWCCY 1 cut(s) 411
PscI ACATGT 2 cut(s) 699, 4528
Psp124BI GAGCTC 2 cut(s) 13, 1824
Psp5II RGGWCCY 1 cut(s) 411
Psp6I CCWGG 6 cut(s) 1386, 1815, 2083, 3660, 4190, 4368
PspFI CCCAGC 1 cut(s) 2004
PspGI CCWGG 6 cut(s) 1386, 1815, 2083, 3660, 4190, 4368
PspN4I GGNNCC 7 cut(s) 412, 463, 1039, 1549, 1572, 2480, 4355
PspPI GGNCC 9 cut(s) 411, 721, 821, 1038, 1548, 1637, 1781, 1943, 3893
PspPPI RGGWCCY 1 cut(s) 411
PstI CTGCAG 3 cut(s) 1582, 2959, 2998
PsuI RGATCY 4 cut(s) 461, 1828, 3580, 4166
PvuII CAGCTG 1 cut(s) 637
SacI GAGCTC 2 cut(s) 13, 1824
SapI GCTCTTC 1 cut(s) 96
Sau96I GGNCC 9 cut(s) 411, 721, 821, 1038, 1548, 1637, 1781, 1943, 3893
ScaI AGTACT 2 cut(s) 2748, 2850
SchI GAGTC 5 cut(s) 216, 1874, 3209, 3454, 4340
ScrFI CCNGG 7 cut(s) 1388, 1817, 2085, 3662, 4192, 4351, 4370
SduI GDGCHC 4 cut(s) 13, 1824, 2172, 3756
SfcI CTRYAG 5 cut(s) 1376, 1578, 2778, 2955, 2994
Sfr274I CTCGAG 1 cut(s) 2483
SfuI TTCGAA 2 cut(s) 3275, 4384
SinI GGWCC 6 cut(s) 411, 821, 1038, 1548, 1637, 1943
SlaI CTCGAG 1 cut(s) 2483
SmlI CTYRAG 6 cut(s) 295, 1220, 1303, 2483, 3086, 3919
SmoI CTYRAG 6 cut(s) 295, 1220, 1303, 2483, 3086, 3919
SpeI ACTAGT 2 cut(s) 116, 4114
SsiI CCGC 5 cut(s) 2312, 3260, 3882, 4401, 4420
SspI AATATT 3 cut(s) 910, 2042, 3476
SstI GAGCTC 2 cut(s) 13, 1824
StyD4I CCNGG 7 cut(s) 1386, 1815, 2083, 3660, 4190, 4349, 4368
StyI CCWWGG 4 cut(s) 1758, 1921, 2451, 3283
TatI WGTACW 6 cut(s) 795, 1395, 2035, 2730, 2746, 2848
TauI GCSGC 3 cut(s) 3884, 4404, 4423
TfiI GAWTC 9 cut(s) 220, 394, 448, 772, 1854, 2828, 2960, 3673, 3924
TscAI CASTG 8 cut(s) 333, 748, 851, 895, 1587, 2286, 3328, 3819
TseFI GTSAC 8 cut(s) 112, 119, 1011, 1077, 1672, 2998, 3956, 4228
TseI GCWGC 9 cut(s) 473, 2096, 2993, 3242, 3809, 3884, 4109, 4295, 4442
Tsp45I GTSAC 8 cut(s) 112, 119, 1011, 1077, 1672, 2998, 3956, 4228
TspGWI ACGGA 2 cut(s) 151, 2718
TspRI CASTG 8 cut(s) 333, 748, 851, 895, 1587, 2286, 3328, 3819
Van91I CCANNNNNTGG 2 cut(s) 1940, 4473
Vha464I CTTAAG 1 cut(s) 3086
VpaK11BI GGWCC 6 cut(s) 411, 821, 1038, 1548, 1637, 1943
XceI RCATGY 5 cut(s) 703, 2470, 2660, 4532, 4585
XcmI CCANNNNNNNNNTGG 4 cut(s) 837, 1246, 1581, 2935
XhoI CTCGAG 1 cut(s) 2483
XmaJI CCTAGG 1 cut(s) 3283
XmiI GTMKAC 2 cut(s) 149, 898
XmnI GAANNNNTTC 8 cut(s) 271, 452, 2190, 2296, 2574, 3188, 3271, 4202
ZrmI AGTACT 2 cut(s) 2748, 2850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.