Rw5G007630

protein At1g04910-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006470
Physical Location & Seq
Reverse (-)
8075395 .. 8078030
2636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G007630.1

Sequence Viewer

Length: 1845 bp
ATGGGAAAGCAAGGGAGCAAAGAATATGATCCTTCTAATATTGGGAGGAGAATGTCAGGGGGAGATTACAGTTTGAATTCCAAAATGTTGTGGCTTCATGGTCTGAAAAGTCATGACTTTCATGTTCTACGAAATGATTCCATGAAGTGTAGTACTTGTAAGGGAAGCTTTGTGGGGAAAAAGCATTTGTGGCTTCGGAAAGATGTGAAGTCAATCGTTTTTGTGTTTGTTTTGATGGGTTTCCTTATCCTGCTTGATACTTTTCTGGTGTCCTTTTTCGATTCTATGGTTCTTCAGAACAGTTCAACTTTAAGAAAATCTCTGGGGCTTAAGGAGGACAAAATGGATGCCTACATTATGAAAGAAAAATCACCAGTTCAGATGTATGATCGCCTACTAAAGTTGGCGTCTGCATCCCTTGCAAAGAAAGAGTTCAAGCAAGAGTCATCAACCTTATGGGAAGAACCAAATAGGAGGCAAGTTGCTGCATGGAAACCTTGTGCGGATAGAAAAGTTTCGAAAAGCCTAGGAAAATATAAGAAAAGTAATGGCTACATAATAGTCAGTGCAAATGGTGGTCTCAATCAACAACGAGTTGCTATTTGCAATGCTGTTGCTGTGGCATCTCTTCTAAATGCTACTCTTGTTCTTCCAAGATTTCTTTACAGCAATGTGTGGAATGATCCGAGTCAATTTGGTGATATTTACCAAGAAGAATATTTTATAGATGTAATGAAGGATGAAGTAAACATTGTCAAGGAACTCCCTTCTCATTTGGAATCACTAGACCTTGAAGCAATTGGTAGCCTTATTACTGATGCAGATCTTGTGAAGGAGGCAAAACCAACTGATTATGTTAGAACTGTGCTTCCTATTCTCTTGAAAAATGGAGTTGTTCACTTCCTGGGATATGGGAATCGACTAGGCTTCGATCCATTGCCTTCTGAGCTTCAGAGACTAAGATGCAAATGCAACTTCCATGCTTTGAAATTCGTGCCCGAAATCCAACGAGTTGGTTCTTTATTGGTTACAAGGATAAGAAAATATGATGCTGCACTGAGTATGTTGGACAAACAATTGCTTGGAAACTTCATGCACAGCATTCACTCAAAACAGCATGATGCTGCTGCAAGAGGCCCGTTCAAATATCTTGCTCTACACTTGAGATTCGAGATTGACATGGTAGCCTATTCCCTCTGTGACTTTGGAGGTGGAGAAAGTGAGAGAAATGAACTTCAAGCATACAGAGAAATCCATTTTCCTCTGCTCATCGAGCGCTTAAAGAACTCGAAGTCTGTTTCTCAAAGTGAGTTGAGACAGTTGGGGAAATGTCCATTGACACCGGAAGAAGCAGCACTAGTTCTTGCTGGTATAGGTTTTAAGCATAGAACTTACATTTACCTAGCTGGATCTCAGATATATAGTGGAAACTCCAGGATGAATTCCTTCACTAACCTCTATCCTAATTTGGTCACAAAGGAAACTCTCCTCACACCAAGAGAACTTGCACCTTTTCAAAACTTCTCTTCCAAGTTAGCAGCACTAGACTTCATTGCCTGTGCAACTGCCGATGTGTTTGCTATGACTGACTCCGGGAGCCAACTATCCTCCCTGGTATCTGGATTTCGAACCTATTATGGTGGCGGCCATGCTCCTACTCTGCGGCCTAACAAGAAGAGGCTAGCAGCAATTTTGTCTGAGAATAGTACCATAGGCTGGAATATTTTTGAAGACAGAGTTAAAAAGATGATCGAGGAAGGTCAAAGGGTACATGTAAGGAGGTTTGGTCGAAGCATTTATCGACAGCCAAGATGCCCAGAGTGCATGTGTAAATCCTACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

614

Amino Acids

69.44

Weight (kDa)

9.38

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 185 - 546 5.4e-93 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1716
AciI CCGC 3 cut(s) 503, 1644, 1663
AclWI GGATC 4 cut(s) 23, 677, 926, 1417
AcoI YGGCCR 1 cut(s) 1645
AcsI RAATTY 3 cut(s) 76, 989, 1441
AcuI CTGAAG 2 cut(s) 278, 935
AcyI GRCGYC 1 cut(s) 407
AfaI GTAC 3 cut(s) 154, 1708, 1770
AfeI AGCGCT 1 cut(s) 1277
AfiI CCNNNNNNNGG 1 cut(s) 1716
AflII CTTAAG 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 1771
AhlI ACTAGT 1 cut(s) 1357
AjnI CCWGG 3 cut(s) 903, 1433, 1611
AloI GAACNNNNNNTCC 2 cut(s) 853, 885
AluBI AGCT 3 cut(s) 168, 949, 1406
AluI AGCT 3 cut(s) 168, 949, 1406
Alw26I GTCTC 3 cut(s) 584, 949, 1309
AlwI GGATC 4 cut(s) 23, 677, 926, 1417
Aor51HI AGCGCT 1 cut(s) 1277
AoxI GGCC 3 cut(s) 1135, 1645, 1664
ApeKI GCWGC 7 cut(s) 485, 1052, 1124, 1127, 1352, 1538, 1685
ApoI RAATTY 3 cut(s) 76, 989, 1441
Asp700I GAANNNNTTC 4 cut(s) 136, 431, 514, 1445
AspA2I CCTAGG 1 cut(s) 526
AspLEI GCGC 1 cut(s) 1278
AspS9I GGNCC 1 cut(s) 1136
AsuC2I CCSGG 1 cut(s) 1594
AsuHPI GGTGA 2 cut(s) 363, 710
AsuII TTCGAA 2 cut(s) 518, 1627
AsuNHI GCTAGC 1 cut(s) 1681
AvrII CCTAGG 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 999
BbsI GAAGAC 1 cut(s) 1737
BbvI GCAGC 7 cut(s) 472, 1039, 1111, 1114, 1364, 1550, 1697
BccI CCATC 1 cut(s) 229
BcgI CGANNNNNNTGC 2 cut(s) 1559, 1593
BciT130I CCWGG 3 cut(s) 905, 1435, 1613
BcnI CCSGG 1 cut(s) 1594
BcoDI GTCTC 3 cut(s) 584, 949, 1309
BcuI ACTAGT 1 cut(s) 1357
BfaI CTAG 7 cut(s) 527, 785, 923, 1358, 1403, 1544, 1682
BfoI RGCGCY 1 cut(s) 1279
BfrI CTTAAG 1 cut(s) 329
BglII AGATCT 1 cut(s) 823
BisI GCNGC 9 cut(s) 486, 1053, 1125, 1128, 1353, 1539, 1645, 1664, 1686
BlnI CCTAGG 1 cut(s) 526
BlsI GCNGC 9 cut(s) 487, 1054, 1126, 1129, 1354, 1540, 1646, 1665, 1687
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 4 cut(s) 905, 1435, 1594, 1613
BmgT120I GGNCC 1 cut(s) 1136
BmiI GGNNCC 1 cut(s) 1598
BmrFI CCNGG 4 cut(s) 905, 1435, 1594, 1613
BmsI GCATC 8 cut(s) 337, 422, 632, 808, 953, 1039, 1111, 1802
BmtI GCTAGC 1 cut(s) 1685
BpiI GAAGAC 1 cut(s) 1737
BpmI CTGGAG 1 cut(s) 1417
Bpu14I TTCGAA 2 cut(s) 518, 1627
BpuEI CTTGAG 1 cut(s) 1183
BpuMI CCSGG 1 cut(s) 1594
BsaBI GATNNNNATC 1 cut(s) 822
BsaHI GRCGYC 1 cut(s) 407
BsaI GGTCTC 1 cut(s) 584
BsaJI CCNNGG 3 cut(s) 526, 904, 1611
BsaWI WCCGGW 1 cut(s) 1342
BsaXI ACNNNNNCTCC 2 cut(s) 1771, 1801
Bsc4I CCNNNNNNNGG 1 cut(s) 1716
Bse1I ACTGG 1 cut(s) 374
Bse3DI GCAATG 4 cut(s) 613, 676, 935, 1551
Bse8I GATNNNNATC 1 cut(s) 822
BseBI CCWGG 3 cut(s) 905, 1435, 1613
BseDI CCNNGG 3 cut(s) 526, 904, 1611
BseGI GGATG 4 cut(s) 352, 413, 745, 1443
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 1 cut(s) 1716
BseMI GCAATG 4 cut(s) 613, 676, 935, 1551
BseMII CTCAG 4 cut(s) 936, 1049, 1427, 1689
BseNI ACTGG 1 cut(s) 374
BseRI GAGGAG 2 cut(s) 61, 1478
BseSI GKGCMC 1 cut(s) 999
BseXI GCAGC 7 cut(s) 472, 1039, 1111, 1114, 1364, 1550, 1697
BsgI GTGCAG 1 cut(s) 1038
BshFI GGCC 3 cut(s) 1137, 1647, 1666
BsiSI CCGG 2 cut(s) 1343, 1593
BslI CCNNNNNNNGG 1 cut(s) 1716
BsmAI GTCTC 3 cut(s) 584, 949, 1309
BsmI GAATGC 1 cut(s) 1101
BsnI GGCC 3 cut(s) 1137, 1647, 1666
Bso31I GGTCTC 1 cut(s) 584
Bsp119I TTCGAA 2 cut(s) 518, 1627
Bsp1286I GDGCHC 1 cut(s) 999
Bsp143I GATC 7 cut(s) 28, 388, 682, 823, 931, 1409, 1749
BspACI CCGC 3 cut(s) 503, 1644, 1663
BspANI GGCC 3 cut(s) 1137, 1647, 1666
BspCNI CTCAG 4 cut(s) 937, 1050, 1426, 1690
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 1598
BspOI GCTAGC 1 cut(s) 1685
BspPI GGATC 4 cut(s) 23, 677, 926, 1417
BspT104I TTCGAA 2 cut(s) 518, 1627
BspTI CTTAAG 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 584
BsrDI GCAATG 4 cut(s) 613, 676, 935, 1551
BsrI ACTGG 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 526, 904, 1611
BssMI GATC 7 cut(s) 28, 388, 682, 823, 931, 1409, 1749
BssNI GRCGYC 1 cut(s) 407
BssT1I CCWWGG 1 cut(s) 526
Bst2UI CCWGG 3 cut(s) 905, 1435, 1613
Bst4CI ACNGT 4 cut(s) 71, 302, 865, 1320
Bst6I CTCTTC 3 cut(s) 633, 1531, 1670
BstACI GRCGYC 1 cut(s) 407
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 419
BstBI TTCGAA 2 cut(s) 518, 1627
BstC8I GCNNGC 1 cut(s) 1683
BstDEI CTNAG 5 cut(s) 945, 959, 1058, 1413, 1698
BstF5I GGATG 4 cut(s) 352, 413, 745, 1443
BstH2I RGCGCY 1 cut(s) 1279
BstHHI GCGC 1 cut(s) 1278
BstKTI GATC 7 cut(s) 31, 391, 685, 826, 934, 1412, 1752
BstMAI GTCTC 3 cut(s) 584, 949, 1309
BstMBI GATC 7 cut(s) 28, 388, 682, 823, 931, 1409, 1749
BstMWI GCNNNNNNNGC 5 cut(s) 190, 419, 946, 1273, 1821
BstNI CCWGG 3 cut(s) 905, 1435, 1613
BstNSI RCATGY 2 cut(s) 1775, 1828
BstSCI CCNGG 4 cut(s) 903, 1433, 1592, 1611
BstSLI GKGCMC 1 cut(s) 999
BstV1I GCAGC 7 cut(s) 472, 1039, 1111, 1114, 1364, 1550, 1697
BstV2I GAAGAC 1 cut(s) 1737
BstX2I RGATCY 2 cut(s) 823, 1409
BstXI CCANNNNNNTGG 1 cut(s) 1013
BstYI RGATCY 2 cut(s) 823, 1409
BsuRI GGCC 3 cut(s) 1137, 1647, 1666
BtsCI GGATG 4 cut(s) 352, 413, 745, 1443
BtsIMutI CAGTG 2 cut(s) 571, 1055
Cac8I GCNNGC 1 cut(s) 1683
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 1278
Cfr13I GGNCC 1 cut(s) 1136
CseI GACGC 1 cut(s) 396
Csp6I GTAC 3 cut(s) 153, 1707, 1769
CviQI GTAC 3 cut(s) 153, 1707, 1769
DdeI CTNAG 5 cut(s) 945, 959, 1058, 1413, 1698
DpnI GATC 7 cut(s) 30, 390, 684, 825, 933, 1411, 1751
DpnII GATC 7 cut(s) 28, 388, 682, 823, 931, 1409, 1749
EaeI YGGCCR 1 cut(s) 1645
Eam1104I CTCTTC 3 cut(s) 633, 1531, 1670
EarI CTCTTC 3 cut(s) 633, 1531, 1670
Eco130I CCWWGG 1 cut(s) 526
Eco31I GGTCTC 1 cut(s) 584
Eco47III AGCGCT 1 cut(s) 1277
Eco57I CTGAAG 2 cut(s) 278, 935
EcoRI GAATTC 2 cut(s) 76, 1441
EcoRII CCWGG 3 cut(s) 903, 1433, 1611
EcoT14I CCWWGG 1 cut(s) 526
ErhI CCWWGG 1 cut(s) 526
FalI AAGNNNNNCTT 2 cut(s) 152, 184
Fnu4HI GCNGC 9 cut(s) 486, 1053, 1125, 1128, 1353, 1539, 1645, 1664, 1686
FokI GGATG 4 cut(s) 359, 400, 752, 1450
Fsp4HI GCNGC 9 cut(s) 486, 1053, 1125, 1128, 1353, 1539, 1645, 1664, 1686
FspBI CTAG 7 cut(s) 527, 785, 923, 1358, 1403, 1544, 1682
GlaI GCGC 1 cut(s) 1277
GluI GCNGC 9 cut(s) 486, 1053, 1125, 1128, 1353, 1539, 1645, 1664, 1686
GsuI CTGGAG 1 cut(s) 1417
HaeII RGCGCY 1 cut(s) 1279
HaeIII GGCC 3 cut(s) 1137, 1647, 1666
HapII CCGG 2 cut(s) 1343, 1593
HgaI GACGC 1 cut(s) 396
HhaI GCGC 1 cut(s) 1278
Hin1I GRCGYC 1 cut(s) 407
Hin6I GCGC 1 cut(s) 1276
HinP1I GCGC 1 cut(s) 1276
HindIII AAGCTT 1 cut(s) 166
HinfI GANTC 8 cut(s) 137, 281, 443, 688, 779, 916, 1167, 1589
HpaII CCGG 2 cut(s) 1343, 1593
HphI GGTGA 2 cut(s) 363, 710
Hpy166II GTNNAC 2 cut(s) 748, 898
Hpy188I TCNGA 9 cut(s) 105, 198, 297, 381, 687, 946, 954, 1416, 1699
Hpy188III TCNNGA 4 cut(s) 113, 880, 1171, 1620
Hpy8I GTNNAC 2 cut(s) 748, 898
HpyAV CCTTC 7 cut(s) 42, 730, 777, 826, 951, 1456, 1751
HpyCH4III ACNGT 4 cut(s) 71, 302, 865, 1320
HpyF10VI GCNNNNNNNGC 5 cut(s) 190, 419, 946, 1273, 1821
HpyF3I CTNAG 5 cut(s) 945, 959, 1058, 1413, 1698
Hsp92I GRCGYC 1 cut(s) 407
HspAI GCGC 1 cut(s) 1276
Kzo9I GATC 7 cut(s) 28, 388, 682, 823, 931, 1409, 1749
LmnI GCTCC 3 cut(s) 15, 1596, 1657
Lsp1109I GCAGC 7 cut(s) 472, 1039, 1111, 1114, 1364, 1550, 1697
LweI GCATC 8 cut(s) 337, 422, 632, 808, 953, 1039, 1111, 1802
MaeI CTAG 7 cut(s) 527, 785, 923, 1358, 1403, 1544, 1682
MaeIII GTNAC 3 cut(s) 1027, 1199, 1471
MalI GATC 7 cut(s) 30, 390, 684, 825, 933, 1411, 1751
MboI GATC 7 cut(s) 28, 388, 682, 823, 931, 1409, 1749
MboII GAAGA 9 cut(s) 284, 473, 620, 641, 725, 1358, 1518, 1687, 1742
MfeI CAATTG 2 cut(s) 798, 1076
MflI RGATCY 2 cut(s) 823, 1409
MhlI GDGCHC 1 cut(s) 999
MluCI AATT 8 cut(s) 76, 692, 798, 989, 1076, 1441, 1465, 1689
MlyI GAGTC 3 cut(s) 452, 697, 1583
MmeI TCCRAC 2 cut(s) 1030, 1047
MroXI GAANNNNTTC 4 cut(s) 136, 431, 514, 1445
MseI TTAA 5 cut(s) 311, 330, 1280, 1380, 1740
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 2 cut(s) 1343, 1593
MspR9I CCNGG 4 cut(s) 905, 1435, 1594, 1613
MunI CAATTG 2 cut(s) 798, 1076
Mva1269I GAATGC 1 cut(s) 1101
MvaI CCWGG 3 cut(s) 905, 1435, 1613
MwoI GCNNNNNNNGC 5 cut(s) 190, 419, 946, 1273, 1821
NciI CCSGG 1 cut(s) 1594
NdeII GATC 7 cut(s) 28, 388, 682, 823, 931, 1409, 1749
NheI GCTAGC 1 cut(s) 1681
NlaIV GGNNCC 1 cut(s) 1598
NmuCI GTSAC 2 cut(s) 1199, 1471
NspI RCATGY 2 cut(s) 1775, 1828
NspV TTCGAA 2 cut(s) 518, 1627
PagI TCATGA 1 cut(s) 112
PciI ACATGT 1 cut(s) 1771
PctI GAATGC 1 cut(s) 1101
PdmI GAANNNNTTC 4 cut(s) 136, 431, 514, 1445
PfeI GAWTC 5 cut(s) 137, 281, 779, 916, 1167
PflMI CCANNNNNTGG 1 cut(s) 1716
PfoI TCCNGGA 2 cut(s) 1433, 1592
PkrI GCNGC 9 cut(s) 487, 1054, 1126, 1129, 1354, 1540, 1646, 1665, 1687
PleI GAGTC 3 cut(s) 451, 696, 1583
PpsI GAGTC 3 cut(s) 451, 696, 1583
PscI ACATGT 1 cut(s) 1771
Psp6I CCWGG 3 cut(s) 903, 1433, 1611
PspGI CCWGG 3 cut(s) 903, 1433, 1611
PspN4I GGNNCC 1 cut(s) 1598
PspPI GGNCC 1 cut(s) 1136
PsuI RGATCY 2 cut(s) 823, 1409
RsaI GTAC 3 cut(s) 154, 1708, 1770
RsaNI GTAC 3 cut(s) 153, 1707, 1769
SaqAI TTAA 5 cut(s) 311, 330, 1280, 1380, 1740
SatI GCNGC 9 cut(s) 486, 1053, 1125, 1128, 1353, 1539, 1645, 1664, 1686
Sau3AI GATC 7 cut(s) 28, 388, 682, 823, 931, 1409, 1749
Sau96I GGNCC 1 cut(s) 1136
ScaI AGTACT 1 cut(s) 154
SchI GAGTC 3 cut(s) 452, 697, 1583
ScrFI CCNGG 4 cut(s) 905, 1435, 1594, 1613
SduI GDGCHC 1 cut(s) 999
SfaNI GCATC 8 cut(s) 337, 422, 632, 808, 953, 1039, 1111, 1802
SfuI TTCGAA 2 cut(s) 518, 1627
SmlI CTYRAG 2 cut(s) 329, 1162
SmoI CTYRAG 2 cut(s) 329, 1162
SpeI ACTAGT 1 cut(s) 1357
Sse9I AATT 8 cut(s) 76, 692, 798, 989, 1076, 1441, 1465, 1689
SsiI CCGC 3 cut(s) 503, 1644, 1663
SspI AATATT 3 cut(s) 40, 719, 1723
SspMI CTAG 7 cut(s) 527, 785, 923, 1358, 1403, 1544, 1682
StyD4I CCNGG 4 cut(s) 903, 1433, 1592, 1611
StyI CCWWGG 1 cut(s) 526
TaaI ACNGT 4 cut(s) 71, 302, 865, 1320
TasI AATT 8 cut(s) 76, 692, 798, 989, 1076, 1441, 1465, 1689
TatI WGTACW 1 cut(s) 152
TauI GCSGC 2 cut(s) 1647, 1666
TfiI GAWTC 5 cut(s) 137, 281, 779, 916, 1167
Tru1I TTAA 5 cut(s) 311, 330, 1280, 1380, 1740
Tru9I TTAA 5 cut(s) 311, 330, 1280, 1380, 1740
TscAI CASTG 2 cut(s) 571, 1062
TseFI GTSAC 2 cut(s) 1199, 1471
TseI GCWGC 7 cut(s) 485, 1052, 1124, 1127, 1352, 1538, 1685
Tsp45I GTSAC 2 cut(s) 1199, 1471
TspRI CASTG 2 cut(s) 571, 1062
Van91I CCANNNNNTGG 1 cut(s) 1716
Vha464I CTTAAG 1 cut(s) 329
XapI RAATTY 3 cut(s) 76, 989, 1441
XceI RCATGY 2 cut(s) 1775, 1828
XmaJI CCTAGG 1 cut(s) 526
XmnI GAANNNNTTC 4 cut(s) 136, 431, 514, 1445
XspI CTAG 7 cut(s) 527, 785, 923, 1358, 1403, 1544, 1682
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.