AT1G29810

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008820
Physical Location & Seq
Reverse (-)
10435374 .. 10437558
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G29810.1

Sequence Viewer

Length: 564 bp
ATGAGTCGGCTCTTGCTACCTAAACTTTTCTCAATCTCTAGAACACAGGTTCCAGCTGCATCATTGTTCAATAATCTGTACAGACGTCACAAACGTTTTGTTCATTGGACGAGTAAGATGTCAACAGATAGTGTTAGATCATCCACAACTGGTGGTTCTGCTTCTGGAGCTAGAACATTTTGCTCCCTCGCAGATTTATCAACCAAAAAGTGTGTGCCATGCAATGCGAAGGATCTGCGCGCCATGACCGAACAAAGTGCCCAAGACCTACTTCAAAAGGTTGCTGGATGGGATTTGGCCAATGACAATGATACATTAAAGCTGCATCGGTCATGGAGGGTGAAAAGTTTTACAAAGGGGCTAGATTTCTTCCAACGTGTAGCTGATATCGCTGAATCAGAAGGTCATCACCCAGATTTGCATCTGGTCGGCTGGAATAATGTGAAAATTGAGATATGGACACATGCGATAGGTGGTTTGACAGAAAACGACTTCATTCTTGCTGCTAAGATCAACGAGCTCCAAGTGGAAGATCTTCTGAGGAAGAAGAAAGTTGCTAAGTGA

Protein Analysis

187

Amino Acids

21.06

Weight (kDa)

9.68

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 81 - 174 1.7e-29 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 88
AccII CGCG 1 cut(s) 240
AclI AACGTT 1 cut(s) 94
AclWI GGATC 1 cut(s) 240
AcoI YGGCCR 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 376
AgsI TTSAA 2 cut(s) 70, 275
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AloI GAACNNNNNNTCC 2 cut(s) 34, 66
AluBI AGCT 5 cut(s) 56, 170, 322, 383, 520
AluI AGCT 5 cut(s) 56, 170, 322, 383, 520
Alw21I GWGCWC 1 cut(s) 522
AlwI GGATC 1 cut(s) 240
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 3 cut(s) 56, 322, 503
Asp700I GAANNNNTTC 1 cut(s) 534
AspLEI GCGC 2 cut(s) 240, 242
AsuHPI GGTGA 2 cut(s) 352, 401
BaeGI GKGCMC 1 cut(s) 262
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 3 cut(s) 43, 309, 490
BccI CCATC 1 cut(s) 282
BcgI CGANNNNNNTGC 4 cut(s) 217, 239, 251, 273
BfaI CTAG 3 cut(s) 39, 171, 362
BglII AGATCT 1 cut(s) 532
BisI GCNGC 3 cut(s) 57, 323, 504
BlsI GCNGC 3 cut(s) 58, 324, 505
BmiI GGNNCC 1 cut(s) 51
BmsI GCATC 3 cut(s) 68, 334, 430
BpmI CTGGAG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 229
Bse8I GATNNNNATC 1 cut(s) 420
BseGI GGATG 2 cut(s) 140, 293
BseJI GATNNNNATC 1 cut(s) 420
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 530
BseNI ACTGG 1 cut(s) 154
BsePI GCGCGC 1 cut(s) 238
BseSI GKGCMC 1 cut(s) 262
BseXI GCAGC 3 cut(s) 43, 309, 490
Bsh1236I CGCG 1 cut(s) 240
BshFI GGCC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 522
BsnI GGCC 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 262, 522
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 4 cut(s) 137, 232, 510, 532
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 1 cut(s) 531
BspFNI CGCG 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 51
BspPI GGATC 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 229
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 1 cut(s) 154
BssHII GCGCGC 1 cut(s) 238
BssMI GATC 4 cut(s) 137, 232, 510, 532
BssNI GRCGYC 1 cut(s) 85
BstACI GRCGYC 1 cut(s) 85
BstAUI TGTACA 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 3 cut(s) 507, 539, 558
BstF5I GGATG 2 cut(s) 140, 293
BstFNI CGCG 1 cut(s) 240
BstHHI GCGC 2 cut(s) 240, 242
BstKTI GATC 4 cut(s) 140, 235, 513, 535
BstMBI GATC 4 cut(s) 137, 232, 510, 532
BstMWI GCNNNNNNNGC 2 cut(s) 167, 389
BstNSI RCATGY 1 cut(s) 467
BstSLI GKGCMC 1 cut(s) 262
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 3 cut(s) 43, 309, 490
BstX2I RGATCY 2 cut(s) 232, 532
BstYI RGATCY 2 cut(s) 232, 532
BsuRI GGCC 1 cut(s) 299
BtsCI GGATG 2 cut(s) 140, 293
Cac8I GCNNGC 1 cut(s) 240
CfoI GCGC 2 cut(s) 240, 242
Csp6I GTAC 1 cut(s) 79
CviAII CATG 4 cut(s) 219, 244, 333, 464
CviJI RGCY 9 cut(s) 10, 56, 170, 299, 322, 361, 383, 432, 520
CviKI_1 RGCY 9 cut(s) 10, 56, 170, 299, 322, 361, 383, 432, 520
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 3 cut(s) 507, 539, 558
DpnI GATC 4 cut(s) 139, 234, 512, 534
DpnII GATC 4 cut(s) 137, 232, 510, 532
EaeI YGGCCR 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 522
Eco32I GATATC 1 cut(s) 388
Eco53kI GAGCTC 1 cut(s) 520
EcoICRI GAGCTC 1 cut(s) 520
EcoRV GATATC 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 522
FaeI CATG 4 cut(s) 222, 247, 336, 467
FaiI YATR 5 cut(s) 220, 245, 334, 457, 465
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FatI CATG 4 cut(s) 218, 243, 332, 463
Fnu4HI GCNGC 3 cut(s) 57, 323, 504
FokI GGATG 2 cut(s) 127, 300
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 3 cut(s) 57, 323, 504
FspBI CTAG 3 cut(s) 39, 171, 362
GlaI GCGC 2 cut(s) 239, 241
GluI GCNGC 3 cut(s) 57, 323, 504
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 1 cut(s) 299
HhaI GCGC 2 cut(s) 240, 242
Hin1I GRCGYC 1 cut(s) 85
Hin1II CATG 4 cut(s) 222, 247, 336, 467
Hin6I GCGC 2 cut(s) 238, 240
HinP1I GCGC 2 cut(s) 238, 240
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 2 cut(s) 4, 395
HphI GGTGA 2 cut(s) 352, 401
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 2 cut(s) 400, 540
Hpy188III TCNNGA 2 cut(s) 39, 165
Hpy8I GTNNAC 1 cut(s) 123
HpyAV CCTTC 2 cut(s) 223, 395
HpyCH4IV ACGT 3 cut(s) 85, 94, 376
HpyCH4V TGCA 4 cut(s) 59, 222, 325, 421
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 389
HpyF3I CTNAG 3 cut(s) 507, 539, 558
HpySE526I ACGT 3 cut(s) 85, 94, 376
Hsp92I GRCGYC 1 cut(s) 85
Hsp92II CATG 4 cut(s) 222, 247, 336, 467
HspAI GCGC 2 cut(s) 238, 240
Kzo9I GATC 4 cut(s) 137, 232, 510, 532
LmnI GCTCC 3 cut(s) 167, 188, 525
LpnPI CCDG 8 cut(s) 32, 66, 135, 150, 270, 410, 418, 426
Lsp1109I GCAGC 3 cut(s) 43, 309, 490
LweI GCATC 3 cut(s) 68, 334, 430
MaeI CTAG 3 cut(s) 39, 171, 362
MaeII ACGT 3 cut(s) 85, 94, 376
MaeIII GTNAC 1 cut(s) 86
MalI GATC 4 cut(s) 139, 234, 512, 534
MboI GATC 4 cut(s) 137, 232, 510, 532
MboII GAAGA 5 cut(s) 361, 527, 542, 556, 559
MflI RGATCY 2 cut(s) 232, 532
MhlI GDGCHC 2 cut(s) 262, 522
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 1 cut(s) 447
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 3 cut(s) 197, 330, 534
Mox20I TGGCCA 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 534
MscI TGGCCA 1 cut(s) 299
MseI TTAA 1 cut(s) 317
Msp20I TGGCCA 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 56
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 2 cut(s) 167, 389
NdeII GATC 4 cut(s) 137, 232, 510, 532
NlaIII CATG 4 cut(s) 222, 247, 336, 467
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 86
NspI RCATGY 1 cut(s) 467
PauI GCGCGC 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 1 cut(s) 395
PkrI GCNGC 3 cut(s) 58, 324, 505
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 522
Psp1406I AACGTT 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 51
PsuI RGATCY 2 cut(s) 232, 532
PteI GCGCGC 1 cut(s) 238
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SacI GAGCTC 1 cut(s) 522
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 3 cut(s) 57, 323, 504
Sau3AI GATC 4 cut(s) 137, 232, 510, 532
SchI GAGTC 1 cut(s) 13
SduI GDGCHC 2 cut(s) 262, 522
SfaNI GCATC 3 cut(s) 68, 334, 430
Sse9I AATT 1 cut(s) 447
SspMI CTAG 3 cut(s) 39, 171, 362
SstI GAGCTC 1 cut(s) 522
TaiI ACGT 3 cut(s) 88, 97, 379
TaqII GACCGA 2 cut(s) 263, 318
TasI AATT 1 cut(s) 447
TatI WGTACW 1 cut(s) 78
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TseFI GTSAC 1 cut(s) 86
TseI GCWGC 3 cut(s) 56, 322, 503
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 2 cut(s) 92, 484
XbaI TCTAGA 1 cut(s) 38
XceI RCATGY 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 3 cut(s) 39, 171, 362
ZraI GACGTC 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.