MD16G1203200.v1.1

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008820
Physical Location & Seq
Reverse (-)
18469931 .. 18473079
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1203200.v1.1.491

Sequence Viewer

Length: 375 bp
ATGGACGATTTGTTAATAAAGAAGTGCGTGTCTTGCGACTCAAAGGACATGCGACCCATGACTGAACAAGCAGCAAAAGGATTAATCACAAAGGTGGACGGATGGAATTTGGTGAGTGAAGGTGATGCTTTGAGACTGAAAAGATCATGGACAGTTAAGACCTTCACAAAAGGACTGGATATGCTTAAGCTCGTAGCTGATGTTGCAGAAGCAGAAGGTCATCATCCAGATCTTCATCTAGTTGGATGGAACAATGTAACAGTCGAGATTTGGACACATGCGGTTGGTGGATTGACCGAGAATGACTTCATACTTGCTGCTAAGATCAACAGAATCAGCATGCAACACCTTCTGAGGCGGAAAGCTACCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
Pterin_4a

Protein Analysis

125

Amino Acids

13.89

Weight (kDa)

6.96

Isoelectric Point (pI)

30.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 19 - 112 1.6e-27 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 281, 358
AcsI RAATTY 1 cut(s) 106
AflII CTTAAG 1 cut(s) 185
AleI CACNNNNGTG 1 cut(s) 92
AluBI AGCT 3 cut(s) 190, 197, 365
AluI AGCT 3 cut(s) 190, 197, 365
Alw26I GTCTC 1 cut(s) 127
ApeKI GCWGC 2 cut(s) 71, 317
ApoI RAATTY 1 cut(s) 106
AseI ATTAAT 1 cut(s) 83
Asp700I GAANNNNTTC 1 cut(s) 305
AsuHPI GGTGA 2 cut(s) 124, 134
BbvI GCAGC 2 cut(s) 83, 304
BccI CCATC 2 cut(s) 96, 240
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 1 cut(s) 239
BfrI CTTAAG 1 cut(s) 185
BglII AGATCT 1 cut(s) 229
BisI GCNGC 2 cut(s) 72, 318
BlsI GCNGC 2 cut(s) 73, 319
BmsI GCATC 1 cut(s) 115
BsaBI GATNNNNATC 1 cut(s) 234
Bse1I ACTGG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 234
BseGI GGATG 3 cut(s) 107, 223, 251
BseJI GATNNNNATC 1 cut(s) 234
BseMII CTCAG 1 cut(s) 344
BseNI ACTGG 1 cut(s) 180
BseXI GCAGC 2 cut(s) 83, 304
BsmAI GTCTC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 143, 229, 324
BspACI CCGC 2 cut(s) 281, 358
BspCNI CTCAG 1 cut(s) 345
BspTI CTTAAG 1 cut(s) 185
BsrI ACTGG 1 cut(s) 180
BssMI GATC 3 cut(s) 143, 229, 324
Bst4CI ACNGT 2 cut(s) 154, 262
BstAFI CTTAAG 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 2 cut(s) 321, 353
BstF5I GGATG 3 cut(s) 107, 223, 251
BstKTI GATC 3 cut(s) 146, 232, 327
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 3 cut(s) 143, 229, 324
BstMWI GCNNNNNNNGC 2 cut(s) 33, 203
BstNSI RCATGY 3 cut(s) 52, 281, 343
BstV1I GCAGC 2 cut(s) 83, 304
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BtsCI GGATG 3 cut(s) 107, 223, 251
Cac8I GCNNGC 1 cut(s) 341
CviAII CATG 5 cut(s) 49, 58, 147, 278, 340
CviJI RGCY 3 cut(s) 190, 197, 365
CviKI_1 RGCY 3 cut(s) 190, 197, 365
DdeI CTNAG 2 cut(s) 321, 353
DpnI GATC 3 cut(s) 145, 231, 326
DpnII GATC 3 cut(s) 143, 229, 324
EciI GGCGGA 1 cut(s) 373
FaeI CATG 5 cut(s) 52, 61, 150, 281, 343
FaiI YATR 7 cut(s) 50, 59, 148, 182, 279, 311, 341
FatI CATG 5 cut(s) 48, 57, 146, 277, 339
Fnu4HI GCNGC 2 cut(s) 72, 318
FokI GGATG 3 cut(s) 114, 210, 258
Fsp4HI GCNGC 2 cut(s) 72, 318
FspBI CTAG 1 cut(s) 239
GluI GCNGC 2 cut(s) 72, 318
Hin1II CATG 5 cut(s) 52, 61, 150, 281, 343
HinfI GANTC 2 cut(s) 38, 333
HphI GGTGA 2 cut(s) 124, 134
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 1 cut(s) 354
Hpy188III TCNNGA 2 cut(s) 227, 265
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 4 cut(s) 113, 172, 209, 359
HpyCH4III ACNGT 2 cut(s) 154, 262
HpyCH4V TGCA 2 cut(s) 206, 343
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 203
HpyF3I CTNAG 2 cut(s) 321, 353
Hsp92II CATG 5 cut(s) 52, 61, 150, 281, 343
Kzo9I GATC 3 cut(s) 143, 229, 324
LpnPI CCDG 2 cut(s) 161, 240
Lsp1109I GCAGC 2 cut(s) 83, 304
LweI GCATC 1 cut(s) 115
MaeI CTAG 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 256
MalI GATC 3 cut(s) 145, 231, 326
MboI GATC 3 cut(s) 143, 229, 324
MboII GAAGA 1 cut(s) 224
MflI RGATCY 1 cut(s) 229
MluCI AATT 1 cut(s) 106
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 1 cut(s) 348
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 4 cut(s) 14, 83, 156, 186
MslI CAYNNNNRTG 1 cut(s) 92
MspCI CTTAAG 1 cut(s) 185
MwoI GCNNNNNNNGC 2 cut(s) 33, 203
NdeII GATC 3 cut(s) 143, 229, 324
NlaIII CATG 5 cut(s) 52, 61, 150, 281, 343
NspI RCATGY 3 cut(s) 52, 281, 343
OliI CACNNNNGTG 1 cut(s) 92
PaeI GCATGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 333
PkrI GCNGC 2 cut(s) 73, 319
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PshBI ATTAAT 1 cut(s) 83
PsuI RGATCY 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 92
SaqAI TTAA 4 cut(s) 14, 83, 156, 186
SatI GCNGC 2 cut(s) 72, 318
Sau3AI GATC 3 cut(s) 143, 229, 324
SchI GAGTC 1 cut(s) 32
SetI ASST 8 cut(s) 96, 124, 164, 192, 199, 220, 351, 367
SfaNI GCATC 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
SphI GCATGC 1 cut(s) 343
Sse9I AATT 1 cut(s) 106
SsiI CCGC 2 cut(s) 281, 358
SspMI CTAG 1 cut(s) 239
TaaI ACNGT 2 cut(s) 154, 262
TaqI TCGA 1 cut(s) 264
TaqII GACCGA 1 cut(s) 311
TasI AATT 1 cut(s) 106
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 4 cut(s) 14, 83, 156, 186
Tru9I TTAA 4 cut(s) 14, 83, 156, 186
TseI GCWGC 2 cut(s) 71, 317
TspDTI ATGAA 2 cut(s) 224, 298
TspGWI ACGGA 1 cut(s) 114
Vha464I CTTAAG 1 cut(s) 185
VspI ATTAAT 1 cut(s) 83
XapI RAATTY 1 cut(s) 106
XceI RCATGY 3 cut(s) 52, 281, 343
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.