Rh5AG100900

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008820
Physical Location & Seq
Forward (+)
9334127 .. 9336239
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG100900.1

Sequence Viewer

Length: 483 bp
ATGAGTCGGATGTTACAGCTTCCATTTCCAAAGGTAGCAGTAGTATCAGTGTTTCAGAACCTGTTTGGAGCTCATGGACGTTCCAGTAGCCGAGTTTATGAGATACTTCAGAACAATGTGAGGGTGTCATCCAGTAGAAAATCAGTTTCTGGATTTAGAACTTTTTGCACTGGCCAAGATTTGTCAACAAAGAAGTGTGTGCCGTGCACCTCAAAGGATATGCGACCTATGACTGAAGAATCAGCAAGTAATTTACTCCCAAAGGTGGATGGATGGAATATGGTGAGTGAAAGTGGTTTGTTGAAACTGAAAAAGTCATGGAGAGTTAAATCCTTCACAAAAGGATTGGAAATGTTTAAGCTCATAGCTGATGTTGCAGAAGCAGAAGGTCATCATCCCGACCTTCATCTTGTTGGATGGAACAATGTGACCATTGAGATCTGGACACATGCAGTTGGTAAGGGCATCCTACGAATGAAGTAA

Protein Analysis

160

Amino Acids

17.92

Weight (kDa)

9.86

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 76 - 154 2.1e-17 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 2 cut(s) 92, 255
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 1 cut(s) 304
AluBI AGCT 4 cut(s) 19, 71, 361, 368
AluI AGCT 4 cut(s) 19, 71, 361, 368
Alw21I GWGCWC 2 cut(s) 73, 209
Alw44I GTGCAC 1 cut(s) 205
AlwNI CAGNNNCTG 2 cut(s) 61, 149
AoxI GGCC 1 cut(s) 172
ApaLI GTGCAC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 174
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 2 cut(s) 73, 209
BccI CCATC 3 cut(s) 263, 267, 411
BceAI ACGGC 1 cut(s) 187
BglII AGATCT 1 cut(s) 438
BmsI GCATC 1 cut(s) 474
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse1I ACTGG 3 cut(s) 84, 132, 175
BseGI GGATG 7 cut(s) 15, 128, 274, 278, 394, 422, 465
BseLI CCNNNNNNNGG 1 cut(s) 265
BseNI ACTGG 3 cut(s) 84, 132, 175
BseSI GKGCMC 1 cut(s) 209
BshFI GGCC 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 73, 209
BslI CCNNNNNNNGG 1 cut(s) 265
BsnI GGCC 1 cut(s) 174
Bsp1286I GDGCHC 2 cut(s) 73, 209
Bsp143I GATC 1 cut(s) 438
BspANI GGCC 1 cut(s) 174
BsrI ACTGG 3 cut(s) 84, 132, 175
BssMI GATC 1 cut(s) 438
BstF5I GGATG 7 cut(s) 15, 128, 274, 278, 394, 422, 465
BstKTI GATC 1 cut(s) 441
BstMBI GATC 1 cut(s) 438
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNSI RCATGY 1 cut(s) 452
BstSLI GKGCMC 1 cut(s) 209
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuRI GGCC 1 cut(s) 174
BtsCI GGATG 7 cut(s) 15, 128, 274, 278, 394, 422, 465
BtsIMutI CAGTG 2 cut(s) 54, 168
CaiI CAGNNNCTG 2 cut(s) 61, 149
CviAII CATG 3 cut(s) 74, 318, 449
CviJI RGCY 6 cut(s) 19, 71, 90, 174, 361, 368
CviKI_1 RGCY 6 cut(s) 19, 71, 90, 174, 361, 368
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
EaeI YGGCCR 1 cut(s) 172
Ecl136II GAGCTC 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 73
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 2 cut(s) 92, 255
EcoICRI GAGCTC 1 cut(s) 71
EcoT38I GRGCYC 1 cut(s) 73
FaeI CATG 3 cut(s) 77, 321, 452
FaiI YATR 8 cut(s) 75, 99, 221, 230, 281, 319, 365, 450
FatI CATG 3 cut(s) 73, 317, 448
FokI GGATG 7 cut(s) 22, 115, 281, 285, 381, 429, 452
FriOI GRGCYC 1 cut(s) 73
HaeIII GGCC 1 cut(s) 174
Hin1II CATG 3 cut(s) 77, 321, 452
HincII GTYRAC 1 cut(s) 186
HindII GTYRAC 1 cut(s) 186
HinfI GANTC 2 cut(s) 4, 239
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 186, 207
Hpy188I TCNGA 3 cut(s) 9, 57, 111
Hpy188III TCNNGA 3 cut(s) 150, 398, 442
Hpy8I GTNNAC 2 cut(s) 186, 207
HpyAV CCTTC 3 cut(s) 343, 380, 413
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 4 cut(s) 168, 207, 377, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 3 cut(s) 77, 321, 452
Kzo9I GATC 1 cut(s) 438
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 6 cut(s) 74, 97, 135, 145, 156, 427
LweI GCATC 1 cut(s) 474
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 2 cut(s) 12, 427
MalI GATC 1 cut(s) 440
MboI GATC 1 cut(s) 438
MboII GAAGA 1 cut(s) 248
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 2 cut(s) 73, 209
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 1 cut(s) 250
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 394
MnlI CCTC 2 cut(s) 114, 220
Mox20I TGGCCA 1 cut(s) 174
MscI TGGCCA 1 cut(s) 174
MseI TTAA 2 cut(s) 327, 357
Msp20I TGGCCA 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 374
NdeII GATC 1 cut(s) 438
NlaIII CATG 3 cut(s) 77, 321, 452
NmeAIII GCCGAG 1 cut(s) 116
NmuCI GTSAC 1 cut(s) 427
NspI RCATGY 1 cut(s) 452
PfeI GAWTC 1 cut(s) 239
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 73
PstNI CAGNNNCTG 2 cut(s) 61, 149
PsuI RGATCY 1 cut(s) 438
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 2 cut(s) 327, 357
Sau3AI GATC 1 cut(s) 438
SchI GAGTC 1 cut(s) 13
SduI GDGCHC 2 cut(s) 73, 209
SfaNI GCATC 1 cut(s) 474
Sse9I AATT 1 cut(s) 250
SstI GAGCTC 1 cut(s) 73
TaiI ACGT 1 cut(s) 82
TasI AATT 1 cut(s) 250
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 2 cut(s) 327, 357
Tru9I TTAA 2 cut(s) 327, 357
TscAI CASTG 2 cut(s) 54, 175
TseFI GTSAC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 1 cut(s) 395
TspRI CASTG 2 cut(s) 54, 175
VneI GTGCAC 1 cut(s) 205
XceI RCATGY 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.