Rroxscaffold_1G00063530

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008820
Physical Location & Seq
Reverse (-)
85393035 .. 85396159
3125 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00063530.1

Sequence Viewer

Length: 606 bp
ATGCAACACATTTACCCACTGTTTGTAACCGCTACAGAGAAACCCATCAGCCCCATATTCATGAGTCGGATGTTACAGCTTCCATTTCCAAAGGTAGCAGTAGTATCAGTGTTTCAGAACCTGTTTGGAGCTCATGGACGTTCCAGTAGCCGAGTTTATGAGATACTTCAGAACAATGTGAGGGTGTCATCCAGTAGAAACTCAGTTTCTGGATTTAGAACTTTTTGCACCGGCCAAGATTTGTCAACAAAGAAGTGTGTGCCGTGCACCTCAAAGGATATGCGACCTATGACTGAAGAATCAGCAAGTAATTTACTCCCAAAGGTGGATGGATGGAATATGGTGAGTGAAAGTGGTTTGTTGAAACTGAAAAAGTCATGGAGAGTTAAATCCTTCACAAAAGGATTGGAAATGTTTAAGCTTATAGCTGATGTTGCAGAAGCAGAAGGTCATCATCCCGACCTTCATCTTGTTGGATGGAACAATGTGACCATTGAGATCTGGACACATGCAGTTGGTGGATTGACTGAGAATGATTTCATACTGGCTGCTAAAATCAATGCACTTGATATGCAAAACCTGATGAGGCGGAAAGCTACTGATTGA

Protein Analysis

201

Amino Acids

22.54

Weight (kDa)

9.35

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 96 - 189 1.8e-26 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 30, 589
AcoI YGGCCR 1 cut(s) 232
AcuI CTGAAG 2 cut(s) 152, 315
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 1 cut(s) 364
AluBI AGCT 5 cut(s) 79, 131, 421, 428, 596
AluI AGCT 5 cut(s) 79, 131, 421, 428, 596
Alw21I GWGCWC 2 cut(s) 133, 269
Alw44I GTGCAC 1 cut(s) 265
AlwNI CAGNNNCTG 2 cut(s) 121, 209
AoxI GGCC 1 cut(s) 232
ApaLI GTGCAC 1 cut(s) 265
ApeKI GCWGC 1 cut(s) 548
Asp700I GAANNNNTTC 1 cut(s) 536
AsuHPI GGTGA 1 cut(s) 355
BaeGI GKGCMC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 133
Bbv12I GWGCWC 2 cut(s) 133, 269
BbvI GCAGC 1 cut(s) 535
BccI CCATC 4 cut(s) 53, 323, 327, 471
BceAI ACGGC 1 cut(s) 247
BfmI CTRYAG 1 cut(s) 33
BglII AGATCT 1 cut(s) 498
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 3 cut(s) 144, 192, 549
BseGI GGATG 6 cut(s) 75, 188, 334, 338, 454, 482
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMII CTCAG 2 cut(s) 216, 519
BseNI ACTGG 3 cut(s) 144, 192, 549
BseSI GKGCMC 1 cut(s) 269
BseXI GCAGC 1 cut(s) 535
BshFI GGCC 1 cut(s) 234
BsiHKAI GWGCWC 2 cut(s) 133, 269
BsiSI CCGG 1 cut(s) 231
BslI CCNNNNNNNGG 1 cut(s) 325
BsnI GGCC 1 cut(s) 234
Bsp1286I GDGCHC 2 cut(s) 133, 269
Bsp143I GATC 1 cut(s) 498
BspACI CCGC 2 cut(s) 30, 589
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 2 cut(s) 215, 520
BspHI TCATGA 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 3 cut(s) 144, 192, 549
BssAI RCCGGY 1 cut(s) 230
BssMI GATC 1 cut(s) 498
Bst4CI ACNGT 1 cut(s) 21
BstDEI CTNAG 2 cut(s) 202, 528
BstF5I GGATG 6 cut(s) 75, 188, 334, 338, 454, 482
BstKTI GATC 1 cut(s) 501
BstMBI GATC 1 cut(s) 498
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNSI RCATGY 1 cut(s) 512
BstSFI CTRYAG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 535
BstX2I RGATCY 1 cut(s) 498
BstYI RGATCY 1 cut(s) 498
BsuRI GGCC 1 cut(s) 234
BtsCI GGATG 6 cut(s) 75, 188, 334, 338, 454, 482
BtsIMutI CAGTG 2 cut(s) 17, 114
CaiI CAGNNNCTG 2 cut(s) 121, 209
CciI TCATGA 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 230
CviAII CATG 4 cut(s) 61, 134, 378, 509
CviJI RGCY 9 cut(s) 51, 79, 131, 150, 234, 421, 428, 548, 596
CviKI_1 RGCY 9 cut(s) 51, 79, 131, 150, 234, 421, 428, 548, 596
DdeI CTNAG 2 cut(s) 202, 528
DpnI GATC 1 cut(s) 500
DpnII GATC 1 cut(s) 498
EaeI YGGCCR 1 cut(s) 232
EciI GGCGGA 1 cut(s) 604
Ecl136II GAGCTC 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 152, 315
EcoICRI GAGCTC 1 cut(s) 131
EcoT38I GRGCYC 1 cut(s) 133
FaeI CATG 4 cut(s) 64, 137, 381, 512
FatI CATG 4 cut(s) 60, 133, 377, 508
Fnu4HI GCNGC 1 cut(s) 549
FokI GGATG 6 cut(s) 82, 175, 341, 345, 441, 489
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 1 cut(s) 549
GluI GCNGC 1 cut(s) 549
HaeIII GGCC 1 cut(s) 234
HapII CCGG 1 cut(s) 231
Hin1II CATG 4 cut(s) 64, 137, 381, 512
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 2 cut(s) 64, 299
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 2 cut(s) 246, 267
Hpy188I TCNGA 3 cut(s) 69, 117, 171
Hpy188III TCNNGA 4 cut(s) 61, 210, 458, 502
Hpy8I GTNNAC 2 cut(s) 246, 267
HpyAV CCTTC 3 cut(s) 403, 440, 473
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 7 cut(s) 4, 228, 267, 437, 512, 563, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 2 cut(s) 202, 528
HpySE526I ACGT 1 cut(s) 139
Hsp92II CATG 4 cut(s) 64, 137, 381, 512
Kzo9I GATC 1 cut(s) 498
LmnI GCTCC 1 cut(s) 128
LpnPI CCDG 8 cut(s) 134, 157, 195, 205, 244, 487, 530, 593
Lsp1109I GCAGC 1 cut(s) 535
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 3 cut(s) 25, 72, 487
MalI GATC 1 cut(s) 500
MboI GATC 1 cut(s) 498
MboII GAAGA 1 cut(s) 308
MflI RGATCY 1 cut(s) 498
MhlI GDGCHC 2 cut(s) 133, 269
MluCI AATT 1 cut(s) 310
MlyI GAGTC 1 cut(s) 73
MmeI TCCRAC 2 cut(s) 47, 454
MnlI CCTC 3 cut(s) 174, 280, 579
MroXI GAANNNNTTC 1 cut(s) 536
MseI TTAA 2 cut(s) 387, 417
MslI CAYNNNNRTG 1 cut(s) 59
MspI CCGG 1 cut(s) 231
MwoI GCNNNNNNNGC 1 cut(s) 434
NdeII GATC 1 cut(s) 498
NlaIII CATG 4 cut(s) 64, 137, 381, 512
NmeAIII GCCGAG 1 cut(s) 176
NmuCI GTSAC 1 cut(s) 487
NspI RCATGY 1 cut(s) 512
PagI TCATGA 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 536
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 72
Psp124BI GAGCTC 1 cut(s) 133
PstNI CAGNNNCTG 2 cut(s) 121, 209
PsuI RGATCY 1 cut(s) 498
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 133
SaqAI TTAA 2 cut(s) 387, 417
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 1 cut(s) 498
SchI GAGTC 1 cut(s) 73
SduI GDGCHC 2 cut(s) 133, 269
SfcI CTRYAG 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 59
Sse9I AATT 1 cut(s) 310
SsiI CCGC 2 cut(s) 30, 589
SstI GAGCTC 1 cut(s) 133
TaaI ACNGT 1 cut(s) 21
TaiI ACGT 1 cut(s) 142
TasI AATT 1 cut(s) 310
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 2 cut(s) 387, 417
Tru9I TTAA 2 cut(s) 387, 417
TscAI CASTG 2 cut(s) 24, 114
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 3 cut(s) 49, 455, 529
TspRI CASTG 2 cut(s) 24, 114
VneI GTGCAC 1 cut(s) 265
XceI RCATGY 1 cut(s) 512
XmnI GAANNNNTTC 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.