FvH4_3g06270

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008820
Physical Location & Seq
Reverse (-)
3608046 .. 3611096
3051 bp
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UTR
Exon/CDS
Intron
FvH4_3g06270.t1

Sequence Viewer

Length: 606 bp
ATGGTTCTTGTTTTGCCAATTTGCCCCACCGATCTTGAGCTTAAAGAAAATCCCATATTCATGAGTCGAATGTTACAGCTTCCAATCCCAAAGGTAGCAGTAGTGTCAGTGTTTCAGAACCTGTTTGGAGCTCATGGACGTTCCAGTAGCCGAGTATATGAGATACTTCAGAACAATGTCGGGGTGTCATCCAGTAGAAACTCAGTTTCTGGATTTAGAACTTTTTGCACTGGCCAAGATTTGTCAACAAAGAAGTGCGTGCCCTGCACCTCAAAGGATATGCGACCTATGACTGAAGAATCAGCAAGTAATCTACTCCCGAAGGTGGATGGATGGAATTTGATAAGTGAAAGTGGTTTGTTGAAACTGAAAAAGTCATGGAGAGTTAAATCCTTTACTAAAGGATTGGAAATGTTTAAGCTCGTAGCTGATGTTGCCGAAGCGGAAGGTCATCATCCAGACCTTCATCTTGTTGGATGGAATAATGTGACCATTGAGATATGGACACATGCAATTGGTGGATTGACAGAGAATGACTTCATACTTGCTGCTAAAATCAATGAACTTGATATGCAAAACCTGATGAGGCGGAAAACTACTGATTGA

Protein Analysis

202

Amino Acids

22.44

Weight (kDa)

8.35

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 96 - 189 7.3e-27 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 443, 589
AcoI YGGCCR 1 cut(s) 232
AcsI RAATTY 1 cut(s) 337
AcuI CTGAAG 2 cut(s) 152, 315
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 1 cut(s) 364
AluBI AGCT 5 cut(s) 40, 79, 131, 421, 428
AluI AGCT 5 cut(s) 40, 79, 131, 421, 428
Alw21I GWGCWC 1 cut(s) 133
AlwNI CAGNNNCTG 2 cut(s) 121, 209
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 1 cut(s) 548
ApoI RAATTY 1 cut(s) 337
Asp700I GAANNNNTTC 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 264
BalI TGGCCA 1 cut(s) 234
BanII GRGCYC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 1 cut(s) 535
BccI CCATC 3 cut(s) 323, 327, 471
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
BpuEI CTTGAG 1 cut(s) 56
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 3 cut(s) 144, 192, 235
BseGI GGATG 5 cut(s) 188, 334, 338, 454, 482
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 3 cut(s) 144, 192, 235
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 1 cut(s) 535
BsgI GTGCAG 1 cut(s) 250
BshFI GGCC 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 325
BsnI GGCC 1 cut(s) 234
Bsp1286I GDGCHC 2 cut(s) 133, 264
Bsp143I GATC 1 cut(s) 31
BspACI CCGC 2 cut(s) 443, 589
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 1 cut(s) 215
BspHI TCATGA 1 cut(s) 60
BsrI ACTGG 3 cut(s) 144, 192, 235
BssMI GATC 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 260
BstDEI CTNAG 1 cut(s) 202
BstF5I GGATG 5 cut(s) 188, 334, 338, 454, 482
BstKTI GATC 1 cut(s) 34
BstMBI GATC 1 cut(s) 31
BstMWI GCNNNNNNNGC 2 cut(s) 264, 434
BstNSI RCATGY 1 cut(s) 512
BstSLI GKGCMC 1 cut(s) 264
BstV1I GCAGC 1 cut(s) 535
BsuRI GGCC 1 cut(s) 234
BtsCI GGATG 5 cut(s) 188, 334, 338, 454, 482
BtsIMutI CAGTG 2 cut(s) 114, 228
Cac8I GCNNGC 1 cut(s) 260
CaiI CAGNNNCTG 2 cut(s) 121, 209
CciI TCATGA 1 cut(s) 60
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 4 cut(s) 61, 134, 378, 509
CviJI RGCY 7 cut(s) 40, 79, 131, 150, 234, 421, 428
CviKI_1 RGCY 7 cut(s) 40, 79, 131, 150, 234, 421, 428
DdeI CTNAG 1 cut(s) 202
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 232
EciI GGCGGA 1 cut(s) 604
Ecl136II GAGCTC 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 152, 315
EcoICRI GAGCTC 1 cut(s) 131
EcoT38I GRGCYC 1 cut(s) 133
FaeI CATG 4 cut(s) 64, 137, 381, 512
FatI CATG 4 cut(s) 60, 133, 377, 508
Fnu4HI GCNGC 1 cut(s) 549
FokI GGATG 5 cut(s) 175, 341, 345, 441, 489
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 1 cut(s) 549
GluI GCNGC 1 cut(s) 549
HaeIII GGCC 1 cut(s) 234
Hin1II CATG 4 cut(s) 64, 137, 381, 512
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HinfI GANTC 2 cut(s) 64, 299
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 2 cut(s) 117, 171
Hpy188III TCNNGA 5 cut(s) 35, 61, 210, 319, 458
Hpy8I GTNNAC 1 cut(s) 246
HpyAV CCTTC 3 cut(s) 316, 440, 473
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 4 cut(s) 228, 267, 512, 574
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 434
HpyF3I CTNAG 1 cut(s) 202
HpySE526I ACGT 1 cut(s) 139
Hsp92II CATG 4 cut(s) 64, 137, 381, 512
Kzo9I GATC 1 cut(s) 31
LmnI GCTCC 1 cut(s) 128
LpnPI CCDG 8 cut(s) 134, 157, 195, 205, 216, 277, 471, 593
Lsp1109I GCAGC 1 cut(s) 535
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 2 cut(s) 72, 487
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MboII GAAGA 1 cut(s) 308
MfeI CAATTG 1 cut(s) 513
MhlI GDGCHC 2 cut(s) 133, 264
MlsI TGGCCA 1 cut(s) 234
MluCI AATT 3 cut(s) 18, 337, 513
MluNI TGGCCA 1 cut(s) 234
MlyI GAGTC 1 cut(s) 73
MmeI TCCRAC 1 cut(s) 454
MnlI CCTC 2 cut(s) 280, 579
Mox20I TGGCCA 1 cut(s) 234
MroXI GAANNNNTTC 1 cut(s) 536
MscI TGGCCA 1 cut(s) 234
MseI TTAA 3 cut(s) 42, 387, 417
MslI CAYNNNNRTG 1 cut(s) 59
Msp20I TGGCCA 1 cut(s) 234
MunI CAATTG 1 cut(s) 513
MwoI GCNNNNNNNGC 2 cut(s) 264, 434
NdeII GATC 1 cut(s) 31
NlaIII CATG 4 cut(s) 64, 137, 381, 512
NmeAIII GCCGAG 1 cut(s) 176
NmuCI GTSAC 1 cut(s) 487
NspI RCATGY 1 cut(s) 512
PagI TCATGA 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 536
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 72
Psp124BI GAGCTC 1 cut(s) 133
PstNI CAGNNNCTG 2 cut(s) 121, 209
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 133
SaqAI TTAA 3 cut(s) 42, 387, 417
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 1 cut(s) 31
SchI GAGTC 1 cut(s) 73
SduI GDGCHC 2 cut(s) 133, 264
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 3 cut(s) 18, 337, 513
SsiI CCGC 2 cut(s) 443, 589
SstI GAGCTC 1 cut(s) 133
TaiI ACGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 67
TasI AATT 3 cut(s) 18, 337, 513
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 3 cut(s) 42, 387, 417
Tru9I TTAA 3 cut(s) 42, 387, 417
TscAI CASTG 2 cut(s) 114, 235
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 4 cut(s) 49, 455, 529, 576
TspRI CASTG 2 cut(s) 114, 235
XapI RAATTY 1 cut(s) 337
XceI RCATGY 1 cut(s) 512
XmnI GAANNNNTTC 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.