AT1G29980

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010097
Physical Location & Seq
Reverse (-)
10503239 .. 10506189
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G29980.2

Sequence Viewer

Length: 1224 bp
ATGGTTCCGAGCAACAATAGATGCAAATGGAGTTCCATTTTCTTGTTTCTGCTCAGTGTCTCCGTTGCCGTCTTAGTCGCCGTCGCTGACGACAAGTCTCCGGCGGTTGAGGATGGTTTAGTGATCAACGGCGACTTCGAAACATCACCGTCAAGTGGCTTTCCTGACGACGGAGTAACTGACGGACCATCCGATATCCCAAGCTGGAAATCCAACGGTACGGTGGAGCTAATAAATTCCGGTCAAAAACAAGGCGGAATGATCCTCATTGTCCCACAAGGCCGTCACGCCGTCCGATTAGGGAACGACGCAGAGATCAGCCAAGATCTAACGGTTGAGAAAGGTTTTGTCTATTCAGTCACGTTCAGCGCTGCTCGCACGTGCGCTCAACTCGAATCGATAAACGTGTCGGTAGCGTCTGTGAACGCAGACGCGGACGATATGCTGGCGTCGCGGAACGTTGATTTGCAAACGCTTTATAGCGTTCAAGGGTGGGACCCTTATGCGTGGGCGTTTGAAGCGGAAGATGATCATGTACGGTTGGTTTTTAAGAATCCGGGTATGGAAGATGATCCGACTTGTGGACCAATCATCGATGACATTGCTATTAAGAAGCTCTTTACTCCTGATAAACCCAAAGATAATGCGGTGATTAATGGAGATTTCGAAGATGGTCCATGGATGTTTAGGAACACGTCTCTTGGTGTTTTACTTCCGACAAACCTCGACGAAGAAATCTCGTCTCTTCCTGGTTGGACTGTTGAATCGAACAGAGCGGTTCGGTTTGTTGACTCGGATCACTTCTCTGTTCCAAAGGGCAAACGAGCCGTCGAGCTACTCTCAGGCAAAGAAGGAATTATTTCACAAATGGTTGAGACCAAAGCAGATAAGCCGTACATATTGTCTTTCTCACTAGGCCACGCAGGTGATAAATGCAAGGAACCATTGGCTATTATGGCATTCGCAGGTGATCAGGCGCAGAACTTTCATTACATGGCGCAAGCGAACTCCAGTTTCGAAAAGGCCGGTTTGAATTTCACGGCTAAGGCTGATCGAACCAGAGTTGCGTTCTACAGTGTTTATTACAATACGAGGACTGATGATATGAGCTCCTTGTGTGGACCTGTGATCGATGACGTTAGAGTGTGGTTCTCCGGGTCGAAGAGAATCGGAGCTGGGTTTGGGTTTTGGGTTTTTGTTCTTCTCGTTGTCGGTTTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

407

Amino Acids

44.3

Weight (kDa)

4.65

Isoelectric Point (pI)

28.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF642 PF04862 3 - 168 3.7e-62 Protein of unknown function (DUF642)
DUF642 PF04862 181 - 346 2.7e-18 Protein of unknown function (DUF642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29980 AT1G29980 AT2G34510 AT2G34510
fragaria_vesca FvH4_3g05680
malus_domestica MD05G1284600.v1.1 MD10G1263000.v1.1
prunus_persica Prupe.4G079800_v2.0.a1
pyrus_communis pycom05g26390 pycom10g21920
rosa_chinensis RchiOBHm_Chr5g0013971
rosa_laevigata RLG00000032056
rosa_multiflora Rmu_co8449395.1_g000001
rosa_roxburghii Rroxscaffold_1G00062750
rosa_rugosa Rorug05G0013600 Rorug05G0013700
rosa_samantha Rh5AG107900 Rh5BG104600 Rh5CG116500 Rh5DG103200
rosa_wichuraiana Rw5G009430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 914, 956
Acc36I ACCTGC 2 cut(s) 914, 956
AccBSI CCGCTC 1 cut(s) 776
AccII CGCG 2 cut(s) 434, 454
AciI CCGC 7 cut(s) 104, 255, 434, 454, 521, 647, 776
AclI AACGTT 1 cut(s) 459
AclWI GGATC 3 cut(s) 256, 566, 804
AcsI RAATTY 2 cut(s) 235, 1033
AcvI CACGTG 1 cut(s) 381
AcyI GRCGYC 1 cut(s) 449
AfaI GTAC 3 cut(s) 220, 537, 896
AfeI AGCGCT 1 cut(s) 370
AfiI CCNNNNNNNGG 3 cut(s) 155, 170, 581
AflIII ACRYGT 2 cut(s) 405, 693
AgsI TTSAA 4 cut(s) 488, 518, 764, 1033
AhdI GACNNNNNGTC 1 cut(s) 94
AjiI CACGTC 1 cut(s) 696
AjnI CCWGG 1 cut(s) 748
AleI CACNNNNGTG 1 cut(s) 924
AluBI AGCT 6 cut(s) 204, 229, 616, 835, 1110, 1175
AluI AGCT 6 cut(s) 204, 229, 616, 835, 1110, 1175
Alw21I GWGCWC 1 cut(s) 1112
Alw26I GTCTC 5 cut(s) 64, 102, 702, 747, 869
AlwI GGATC 3 cut(s) 256, 566, 804
Aor51HI AGCGCT 1 cut(s) 370
AoxI GGCC 3 cut(s) 280, 916, 1023
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 2 cut(s) 235, 1033
ArsI GACNNNNNNTTYG 2 cut(s) 813, 845
AseI ATTAAT 1 cut(s) 654
Asp700I GAANNNNTTC 1 cut(s) 859
AspLEI GCGC 4 cut(s) 371, 386, 979, 1000
AspS9I GGNCC 5 cut(s) 185, 496, 584, 674, 1121
AsuC2I CCSGG 2 cut(s) 558, 1156
AsuHPI GGTGA 4 cut(s) 138, 661, 938, 980
AsuII TTCGAA 3 cut(s) 138, 666, 1017
AvaII GGWCC 5 cut(s) 185, 496, 584, 674, 1121
BanII GRGCYC 1 cut(s) 1112
BbrPI CACGTG 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 1112
BbvI GCAGC 1 cut(s) 358
BccI CCATC 3 cut(s) 107, 196, 665
BceAI ACGGC 8 cut(s) 53, 65, 145, 267, 275, 812, 877, 1056
BcgI CGANNNNNNTGC 2 cut(s) 584, 618
BciT130I CCWGG 1 cut(s) 750
BclI TGATCA 3 cut(s) 123, 529, 970
BcnI CCSGG 2 cut(s) 558, 1156
BcoDI GTCTC 5 cut(s) 64, 102, 702, 747, 869
BfaI CTAG 1 cut(s) 914
BfmI CTRYAG 1 cut(s) 1072
BfoI RGCGCY 1 cut(s) 372
BfuAI ACCTGC 2 cut(s) 914, 956
BglII AGATCT 1 cut(s) 325
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
Bme1390I CCNGG 3 cut(s) 558, 750, 1156
Bme18I GGWCC 5 cut(s) 185, 496, 584, 674, 1121
BmeRI GACNNNNNGTC 1 cut(s) 94
BmgBI CACGTC 1 cut(s) 696
BmgT120I GGNCC 5 cut(s) 185, 496, 584, 674, 1121
BmiI GGNNCC 4 cut(s) 6, 497, 498, 942
BmrFI CCNGG 3 cut(s) 558, 750, 1156
BmsI GCATC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 824, 856
BpmI CTGGAG 1 cut(s) 994
Bpu10I CCTNAGC 1 cut(s) 1044
Bpu14I TTCGAA 3 cut(s) 138, 666, 1017
BpuMI CCSGG 2 cut(s) 558, 1156
Bsa29I ATCGAT 3 cut(s) 398, 594, 1131
BsaAI YACGTR 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 449
BsaI GGTCTC 1 cut(s) 869
BsaJI CCNNGG 1 cut(s) 677
BsaWI WCCGGW 1 cut(s) 239
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 170, 581
Bse118I RCCGGY 1 cut(s) 1025
Bse1I ACTGG 1 cut(s) 1011
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 1 cut(s) 750
BseCI ATCGAT 3 cut(s) 398, 594, 1131
BseDI CCNNGG 1 cut(s) 677
BseGI GGATG 3 cut(s) 118, 188, 687
BseLI CCNNNNNNNGG 3 cut(s) 155, 170, 581
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 2 cut(s) 67, 855
BseNI ACTGG 1 cut(s) 1011
BseXI GCAGC 1 cut(s) 358
BseYI CCCAGC 1 cut(s) 1175
Bsh1236I CGCG 2 cut(s) 434, 454
BshFI GGCC 3 cut(s) 282, 918, 1025
BshVI ATCGAT 3 cut(s) 398, 594, 1131
BsiHKAI GWGCWC 1 cut(s) 1112
BsiSI CCGG 5 cut(s) 101, 240, 557, 1026, 1155
BslFI GGGAC 2 cut(s) 257, 509
BslI CCNNNNNNNGG 3 cut(s) 155, 170, 581
BsmAI GTCTC 5 cut(s) 64, 102, 702, 747, 869
BsmBI CGTCTC 2 cut(s) 702, 747
BsmFI GGGAC 2 cut(s) 257, 509
BsmI GAATGC 1 cut(s) 959
BsnI GGCC 3 cut(s) 282, 918, 1025
Bso31I GGTCTC 1 cut(s) 869
Bsp119I TTCGAA 3 cut(s) 138, 666, 1017
Bsp1286I GDGCHC 1 cut(s) 1112
Bsp19I CCATGG 1 cut(s) 677
BspACI CCGC 7 cut(s) 104, 255, 434, 454, 521, 647, 776
BspANI GGCC 3 cut(s) 282, 918, 1025
BspCNI CTCAG 2 cut(s) 66, 854
BspDI ATCGAT 3 cut(s) 398, 594, 1131
BspFNI CGCG 2 cut(s) 434, 454
BspLI GGNNCC 4 cut(s) 6, 497, 498, 942
BspMI ACCTGC 2 cut(s) 914, 956
BspPI GGATC 3 cut(s) 256, 566, 804
BspT104I TTCGAA 3 cut(s) 138, 666, 1017
BspTNI GGTCTC 1 cut(s) 869
BsrBI CCGCTC 1 cut(s) 776
BsrDI GCAATG 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 1025
BsrI ACTGG 1 cut(s) 1011
BssAI RCCGGY 1 cut(s) 1025
BssECI CCNNGG 1 cut(s) 677
BssNI GRCGYC 1 cut(s) 449
BssT1I CCWWGG 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 750
Bst4CI ACNGT 7 cut(s) 150, 218, 223, 334, 540, 760, 1076
Bst6I CTCTTC 2 cut(s) 750, 1157
BstACI GRCGYC 1 cut(s) 449
BstBAI YACGTR 1 cut(s) 381
BstBI TTCGAA 3 cut(s) 138, 666, 1017
BstC8I GCNNGC 3 cut(s) 376, 447, 1002
BstDEI CTNAG 4 cut(s) 53, 73, 841, 1044
BstDSI CCRYGG 1 cut(s) 677
BstF5I GGATG 3 cut(s) 118, 188, 687
BstFNI CGCG 2 cut(s) 434, 454
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 4 cut(s) 371, 386, 979, 1000
BstMAI GTCTC 5 cut(s) 64, 102, 702, 747, 869
BstMWI GCNNNNNNNGC 4 cut(s) 375, 451, 518, 956
BstNI CCWGG 1 cut(s) 750
BstSCI CCNGG 3 cut(s) 556, 748, 1154
BstSFI CTRYAG 1 cut(s) 1072
BstUI CGCG 2 cut(s) 434, 454
BstV1I GCAGC 1 cut(s) 358
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
Bsu15I ATCGAT 3 cut(s) 398, 594, 1131
BsuRI GGCC 3 cut(s) 282, 918, 1025
BsuTUI ATCGAT 3 cut(s) 398, 594, 1131
BtgI CCRYGG 1 cut(s) 677
BtrI CACGTC 1 cut(s) 696
BtsCI GGATG 3 cut(s) 118, 188, 687
BtsIMutI CAGTG 2 cut(s) 61, 1081
BveI ACCTGC 2 cut(s) 914, 956
Cac8I GCNNGC 3 cut(s) 376, 447, 1002
CfoI GCGC 4 cut(s) 371, 386, 979, 1000
Cfr10I RCCGGY 1 cut(s) 1025
Cfr13I GGNCC 5 cut(s) 185, 496, 584, 674, 1121
ClaI ATCGAT 3 cut(s) 398, 594, 1131
CseI GACGC 4 cut(s) 317, 405, 438, 440
Csp6I GTAC 3 cut(s) 219, 536, 895
CviAII CATG 3 cut(s) 533, 678, 994
CviQI GTAC 3 cut(s) 219, 536, 895
DdeI CTNAG 4 cut(s) 53, 73, 841, 1044
DriI GACNNNNNGTC 1 cut(s) 94
Eam1104I CTCTTC 2 cut(s) 750, 1157
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 2 cut(s) 750, 1157
EciI GGCGGA 1 cut(s) 270
Ecl136II GAGCTC 1 cut(s) 1110
Eco130I CCWWGG 1 cut(s) 677
Eco24I GRGCYC 1 cut(s) 1112
Eco31I GGTCTC 1 cut(s) 869
Eco32I GATATC 1 cut(s) 196
Eco47I GGWCC 5 cut(s) 185, 496, 584, 674, 1121
Eco47III AGCGCT 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 1110
Eco72I CACGTG 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 1110
EcoO109I RGGNCCY 1 cut(s) 496
EcoRII CCWGG 1 cut(s) 748
EcoRV GATATC 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 677
EcoT38I GRGCYC 1 cut(s) 1112
ErhI CCWWGG 1 cut(s) 677
Esp3I CGTCTC 2 cut(s) 702, 747
FaeI CATG 3 cut(s) 536, 681, 997
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FaqI GGGAC 2 cut(s) 257, 509
FatI CATG 3 cut(s) 532, 677, 993
FbaI TGATCA 3 cut(s) 123, 529, 970
Fnu4HI GCNGC 1 cut(s) 372
FokI GGATG 3 cut(s) 125, 175, 694
FriOI GRGCYC 1 cut(s) 1112
Fsp4HI GCNGC 1 cut(s) 372
FspBI CTAG 1 cut(s) 914
GlaI GCGC 4 cut(s) 370, 385, 978, 999
GluI GCNGC 1 cut(s) 372
GsaI CCCAGC 1 cut(s) 1179
GsuI CTGGAG 1 cut(s) 994
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 3 cut(s) 282, 918, 1025
HapII CCGG 5 cut(s) 101, 240, 557, 1026, 1155
HgaI GACGC 4 cut(s) 317, 405, 438, 440
HhaI GCGC 4 cut(s) 371, 386, 979, 1000
Hin1I GRCGYC 1 cut(s) 449
Hin1II CATG 3 cut(s) 536, 681, 997
Hin6I GCGC 4 cut(s) 369, 384, 977, 998
HinP1I GCGC 4 cut(s) 369, 384, 977, 998
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HinfI GANTC 5 cut(s) 395, 553, 764, 791, 1167
HpaII CCGG 5 cut(s) 101, 240, 557, 1026, 1155
HphI GGTGA 4 cut(s) 138, 661, 938, 980
Hpy166II GTNNAC 4 cut(s) 424, 584, 790, 1121
Hpy188I TCNGA 7 cut(s) 9, 193, 296, 576, 717, 796, 1172
Hpy188III TCNNGA 2 cut(s) 164, 626
Hpy8I GTNNAC 4 cut(s) 424, 584, 790, 1121
Hpy99I CGWCG 6 cut(s) 86, 173, 311, 454, 731, 833
HpyAV CCTTC 1 cut(s) 845
HpyCH4III ACNGT 7 cut(s) 150, 218, 223, 334, 540, 760, 1076
HpyCH4IV ACGT 6 cut(s) 362, 380, 405, 459, 695, 1137
HpyCH4V TGCA 3 cut(s) 24, 469, 936
HpyF10VI GCNNNNNNNGC 4 cut(s) 375, 451, 518, 956
HpyF3I CTNAG 4 cut(s) 53, 73, 841, 1044
HpySE526I ACGT 6 cut(s) 362, 380, 405, 459, 695, 1137
Hsp92I GRCGYC 1 cut(s) 449
Hsp92II CATG 3 cut(s) 536, 681, 997
HspAI GCGC 4 cut(s) 369, 384, 977, 998
KflI GGGWCCC 1 cut(s) 496
Ksp22I TGATCA 3 cut(s) 123, 529, 970
LmnI GCTCC 3 cut(s) 226, 1115, 1172
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 1 cut(s) 11
MaeI CTAG 1 cut(s) 914
MaeII ACGT 6 cut(s) 362, 380, 405, 459, 695, 1137
MaeIII GTNAC 3 cut(s) 175, 284, 358
MbiI CCGCTC 1 cut(s) 776
MboII GAAGA 7 cut(s) 536, 578, 680, 737, 743, 1174, 1193
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 1 cut(s) 1112
MluCI AATT 3 cut(s) 235, 855, 1033
MlyI GAGTC 1 cut(s) 785
MmeI TCCRAC 4 cut(s) 237, 599, 734, 740
MnlI CCTC 4 cut(s) 103, 275, 734, 1086
MroXI GAANNNNTTC 1 cut(s) 859
MseI TTAA 4 cut(s) 549, 609, 654, 1222
MslI CAYNNNNRTG 1 cut(s) 924
MspI CCGG 5 cut(s) 101, 240, 557, 1026, 1155
MspR9I CCNGG 3 cut(s) 558, 750, 1156
Mva1269I GAATGC 1 cut(s) 959
MvaI CCWGG 1 cut(s) 750
MvnI CGCG 2 cut(s) 434, 454
MwoI GCNNNNNNNGC 4 cut(s) 375, 451, 518, 956
NciI CCSGG 2 cut(s) 558, 1156
NcoI CCATGG 1 cut(s) 677
NlaIII CATG 3 cut(s) 536, 681, 997
NlaIV GGNNCC 4 cut(s) 6, 497, 498, 942
NmuCI GTSAC 2 cut(s) 284, 358
NspV TTCGAA 3 cut(s) 138, 666, 1017
OliI CACNNNNGTG 1 cut(s) 924
PaqCI CACCTGC 2 cut(s) 914, 956
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 189
PctI GAATGC 1 cut(s) 959
PdmI GAANNNNTTC 1 cut(s) 859
PfeI GAWTC 4 cut(s) 395, 553, 764, 1167
PkrI GCNGC 1 cut(s) 373
PleI GAGTC 1 cut(s) 785
PmaCI CACGTG 1 cut(s) 381
PmlI CACGTG 1 cut(s) 381
PpsI GAGTC 1 cut(s) 785
Ppu21I YACGTR 1 cut(s) 381
PpuMI RGGWCCY 1 cut(s) 496
PshBI ATTAAT 1 cut(s) 654
Psp124BI GAGCTC 1 cut(s) 1112
Psp1406I AACGTT 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 496
Psp6I CCWGG 1 cut(s) 748
PspCI CACGTG 1 cut(s) 381
PspFI CCCAGC 1 cut(s) 1175
PspGI CCWGG 1 cut(s) 748
PspN4I GGNNCC 4 cut(s) 6, 497, 498, 942
PspPI GGNCC 5 cut(s) 185, 496, 584, 674, 1121
PspPPI RGGWCCY 1 cut(s) 496
PsrI GAACNNNNNNTAC 2 cut(s) 974, 1006
PsuI RGATCY 1 cut(s) 325
RsaI GTAC 3 cut(s) 220, 537, 896
RsaNI GTAC 3 cut(s) 219, 536, 895
RseI CAYNNNNRTG 1 cut(s) 924
SacI GAGCTC 1 cut(s) 1112
SaqAI TTAA 4 cut(s) 549, 609, 654, 1222
SatI GCNGC 1 cut(s) 372
Sau96I GGNCC 5 cut(s) 185, 496, 584, 674, 1121
SchI GAGTC 1 cut(s) 785
ScrFI CCNGG 3 cut(s) 558, 750, 1156
SduI GDGCHC 1 cut(s) 1112
SfaNI GCATC 1 cut(s) 11
SfcI CTRYAG 1 cut(s) 1072
SfuI TTCGAA 3 cut(s) 138, 666, 1017
SinI GGWCC 5 cut(s) 185, 496, 584, 674, 1121
SmiMI CAYNNNNRTG 1 cut(s) 924
Sse9I AATT 3 cut(s) 235, 855, 1033
SsiI CCGC 7 cut(s) 104, 255, 434, 454, 521, 647, 776
SspMI CTAG 1 cut(s) 914
SstI GAGCTC 1 cut(s) 1112
StyD4I CCNGG 3 cut(s) 556, 748, 1154
StyI CCWWGG 1 cut(s) 677
TaaI ACNGT 7 cut(s) 150, 218, 223, 334, 540, 760, 1076
TaiI ACGT 6 cut(s) 365, 383, 408, 462, 698, 1140
TasI AATT 3 cut(s) 235, 855, 1033
TfiI GAWTC 4 cut(s) 395, 553, 764, 1167
Tru1I TTAA 4 cut(s) 549, 609, 654, 1222
Tru9I TTAA 4 cut(s) 549, 609, 654, 1222
TscAI CASTG 2 cut(s) 61, 1081
TseFI GTSAC 2 cut(s) 284, 358
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 2 cut(s) 284, 358
TspDTI ATGAA 1 cut(s) 977
TspGWI ACGGA 3 cut(s) 52, 186, 198
TspRI CASTG 2 cut(s) 61, 1081
VpaK11BI GGWCC 5 cut(s) 185, 496, 584, 674, 1121
VspI ATTAAT 1 cut(s) 654
XapI RAATTY 2 cut(s) 235, 1033
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XmnI GAANNNNTTC 1 cut(s) 859
XspI CTAG 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.