MD05G1284600.v1.1

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010097
Physical Location & Seq
Forward (+)
41856335 .. 41861637
5303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1284600.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGGTCGGAGCTCCACATTACACAAATGGGTCTTACCGACGCTGCTTCTTCTCGGTCATTTGGCTGCTGAAATTTCAGCAGATGACGGGCCGGTGCCAAACGGAGATTTTGAACTGACCCCATCAGGCGGCTTTTCAAACGACGGTGCTGTGGACGGGCCCGCCGGGATCCCCAGCTGGAAAGCAAACGGCACCGTCGAGCTTGTCGAATCCGGGCAGAAACAGGGTGGGATGATCCTCATCGTACCGCAAGGTAGACACGCGGTGAGGCTCGGAAACGACGCCGAAATAAGCCAGCAAGTGAAGGTGGAGAAAGGGTCGATATACTCGGTGACTTTCAGCGCGGCCCGCACGTGCGCACAGCTGGAGTCGCTGAACGTGTCCGTGCCACCTGCGTCGCAGACGATAGACCTTCAGACCTTGTACAACGTGCAGGGGTGGGACCCCTACGCGTGGGCCTTCGAGGCGGAGGAGGATGATGCGATGTTGGTCTTCAGGAATCCCGGCATGGAGGATGACCCCACTTGTGGGCCCATCATTGATGACGTTGCTATGAAGAAACTCTTTACCCCTGATAGGCCCAAAGACAACGCAGTGATCAATGGTGATTTTGAGGAAGGTCCATGGATGTTTCCAAACATCTCACTCGGAGTCCTGCTCCCAACCAACCTCGACGAAGAAACTTCTTCCTTACCCGGTTGGATCGTCGAGTCCAACCGGGCTGTTAGGTACATAGACTCGTACCATTACGCAGTGCCACAAGGCAAACGGGCCATTGAATTGGTATCTGGAAAAGAAGGCATCATATCTCAAATGGTTGAAACTTCACCAAACAAGCCCTACACCTTGACCTTCTCATTAGGCCATGCTAATGACAAATGCAAGCAGCCTCTCGGCATCATGGCATTCGCCGGAGACCAGGCGCAAAACGTCCATTACACACCGAATTCCAACAGCACGTTCCAAACCGCCGGCGTGAATTTCACGGCCAAGGCTGAGAGGACAAGGATTGCATTCTACAGTGTGTATTACAACACCAGGACTGATGACATGAGCTCACTCTGTGGGCCTGTGGTGGATGATGTCCGGGTTTGGTTTTCCGGGTCATGGAGAAATGGGCTCGGTGGGTCGGGCCGGGTGGCACTTGGGCTTGCTTTCTGGGCTTTTGTGTGGGTTTTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

395

Amino Acids

42.8

Weight (kDa)

4.78

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF642 PF04862 31 - 186 5.4e-64 Protein of unknown function (DUF642)
DUF642 PF04862 199 - 364 3.6e-19 Protein of unknown function (DUF642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29980 AT1G29980 AT2G34510 AT2G34510
fragaria_vesca FvH4_3g05680
malus_domestica MD05G1284600.v1.1 MD10G1263000.v1.1
prunus_persica Prupe.4G079800_v2.0.a1
pyrus_communis pycom05g26390 pycom10g21920
rosa_chinensis RchiOBHm_Chr5g0013971
rosa_laevigata RLG00000032056
rosa_multiflora Rmu_co8449395.1_g000001
rosa_roxburghii Rroxscaffold_1G00062750
rosa_rugosa Rorug05G0013600 Rorug05G0013700
rosa_samantha Rh5AG107900 Rh5BG104600 Rh5CG116500 Rh5DG103200
rosa_wichuraiana Rw5G009430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
Acc16I TGCGCA 1 cut(s) 358
Acc36I ACCTGC 1 cut(s) 400
AccB1I GGYRCC 2 cut(s) 94, 191
AccI GTMKAC 1 cut(s) 256
AccII CGCG 3 cut(s) 263, 344, 452
AciI CCGC 8 cut(s) 129, 162, 248, 263, 344, 349, 467, 969
AclWI GGATC 4 cut(s) 163, 176, 229, 710
AcoI YGGCCR 1 cut(s) 987
AcsI RAATTY 3 cut(s) 72, 946, 979
AcuI CTGAAG 2 cut(s) 398, 478
AcvI CACGTG 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 282
AdeI CACNNNGTG 2 cut(s) 265, 1064
AfaI GTAC 4 cut(s) 246, 425, 731, 743
AfiI CCNNNNNNNGG 6 cut(s) 128, 453, 527, 528, 576, 1107
AflIII ACRYGT 2 cut(s) 378, 450
AgsI TTSAA 4 cut(s) 113, 138, 779, 821
AjnI CCWGG 2 cut(s) 918, 1037
AjuI GAANNNNNNNTTGG 2 cut(s) 944, 976
AluBI AGCT 5 cut(s) 12, 177, 202, 364, 1056
AluI AGCT 5 cut(s) 12, 177, 202, 364, 1056
Alw21I GWGCWC 2 cut(s) 14, 1058
Alw26I GTCTC 1 cut(s) 909
AlwI GGATC 4 cut(s) 163, 176, 229, 710
ApaI GGGCCC 2 cut(s) 162, 534
ApeKI GCWGC 3 cut(s) 43, 65, 886
ApoI RAATTY 3 cut(s) 72, 946, 979
AspLEI GCGC 3 cut(s) 344, 359, 925
AsuC2I CCSGG 8 cut(s) 166, 214, 504, 696, 719, 1088, 1102, 1136
AsuHPI GGTGA 4 cut(s) 277, 343, 617, 819
AvaII GGWCC 2 cut(s) 442, 620
BaeGI GKGCMC 2 cut(s) 162, 534
BamHI GGATCC 1 cut(s) 168
BanI GGYRCC 2 cut(s) 94, 191
BanII GRGCYC 5 cut(s) 14, 162, 534, 1058, 1122
BbrPI CACGTG 1 cut(s) 354
BbsI GAAGAC 1 cut(s) 484
Bbv12I GWGCWC 2 cut(s) 14, 1058
BbvI GCAGC 3 cut(s) 30, 52, 898
BccI CCATC 2 cut(s) 130, 542
BceAI ACGGC 2 cut(s) 205, 1002
BciT130I CCWGG 2 cut(s) 920, 1039
BclI TGATCA 1 cut(s) 597
BcnI CCSGG 8 cut(s) 166, 214, 504, 696, 719, 1088, 1102, 1136
BcoDI GTCTC 1 cut(s) 909
BfmI CTRYAG 1 cut(s) 1018
BfuAI ACCTGC 1 cut(s) 400
BglI GCCNNNNNGGC 1 cut(s) 464
BisI GCNGC 5 cut(s) 44, 66, 130, 345, 887
BlsI GCNGC 5 cut(s) 45, 67, 131, 346, 888
Bme18I GGWCC 2 cut(s) 442, 620
BmiI GGNNCC 7 cut(s) 96, 160, 170, 193, 443, 444, 532
BmsI GCATC 3 cut(s) 469, 810, 906
BpiI GAAGAC 1 cut(s) 484
BplI GAGNNNNNCTC 2 cut(s) 642, 674
BpmI CTGGAG 1 cut(s) 386
BpuMI CCSGG 8 cut(s) 166, 214, 504, 696, 719, 1088, 1102, 1136
BsaAI YACGTR 1 cut(s) 354
BsaBI GATNNNNATC 1 cut(s) 239
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 909
BsaJI CCNNGG 2 cut(s) 623, 990
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 453, 527, 528, 576, 1107
Bse118I RCCGGY 2 cut(s) 91, 971
Bse8I GATNNNNATC 1 cut(s) 239
BseBI CCWGG 2 cut(s) 920, 1039
BseDI CCNNGG 2 cut(s) 623, 990
BseGI GGATG 5 cut(s) 237, 481, 520, 633, 1084
BseJI GATNNNNATC 1 cut(s) 239
BseLI CCNNNNNNNGG 6 cut(s) 128, 453, 527, 528, 576, 1107
BseMII CTCAG 1 cut(s) 987
BseRI GAGGAG 1 cut(s) 485
BseSI GKGCMC 2 cut(s) 162, 534
BseXI GCAGC 3 cut(s) 30, 52, 898
BseYI CCCAGC 1 cut(s) 173
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 3 cut(s) 263, 344, 452
BshNI GGYRCC 2 cut(s) 94, 191
BsiHKAI GWGCWC 2 cut(s) 14, 1058
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 6 cut(s) 128, 453, 527, 528, 576, 1107
BsmAI GTCTC 1 cut(s) 909
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 2 cut(s) 905, 1013
Bso31I GGTCTC 1 cut(s) 909
Bsp120I GGGCCC 2 cut(s) 158, 530
Bsp1286I GDGCHC 5 cut(s) 14, 162, 534, 1058, 1122
Bsp1407I TGTACA 1 cut(s) 423
Bsp143I GATC 4 cut(s) 168, 234, 597, 702
Bsp19I CCATGG 1 cut(s) 623
BspACI CCGC 8 cut(s) 129, 162, 248, 263, 344, 349, 467, 969
BspCNI CTCAG 1 cut(s) 988
BspFNI CGCG 3 cut(s) 263, 344, 452
BspLI GGNNCC 7 cut(s) 96, 160, 170, 193, 443, 444, 532
BspMI ACCTGC 1 cut(s) 400
BspPI GGATC 4 cut(s) 163, 176, 229, 710
BspT107I GGYRCC 2 cut(s) 94, 191
BspTNI GGTCTC 1 cut(s) 909
BsrFI RCCGGY 2 cut(s) 91, 971
BsrGI TGTACA 1 cut(s) 423
BssAI RCCGGY 2 cut(s) 91, 971
BssECI CCNNGG 2 cut(s) 623, 990
BssMI GATC 4 cut(s) 168, 234, 597, 702
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 2 cut(s) 623, 990
Bst2UI CCWGG 2 cut(s) 920, 1039
Bst4CI ACNGT 3 cut(s) 146, 196, 1022
BstACI GRCGYC 1 cut(s) 282
BstAUI TGTACA 1 cut(s) 423
BstBAI YACGTR 1 cut(s) 354
BstC8I GCNNGC 6 cut(s) 162, 296, 349, 884, 973, 1152
BstDEI CTNAG 1 cut(s) 996
BstDSI CCRYGG 1 cut(s) 623
BstF5I GGATG 5 cut(s) 237, 481, 520, 633, 1084
BstFNI CGCG 3 cut(s) 263, 344, 452
BstHHI GCGC 3 cut(s) 344, 359, 925
BstKTI GATC 4 cut(s) 171, 237, 600, 705
BstMAI GTCTC 1 cut(s) 909
BstMBI GATC 4 cut(s) 168, 234, 597, 702
BstMWI GCNNNNNNNGC 4 cut(s) 348, 370, 464, 1160
BstNI CCWGG 2 cut(s) 920, 1039
BstSFI CTRYAG 1 cut(s) 1018
BstSLI GKGCMC 2 cut(s) 162, 534
BstUI CGCG 3 cut(s) 263, 344, 452
BstV1I GCAGC 3 cut(s) 30, 52, 898
BstV2I GAAGAC 1 cut(s) 484
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 623
BtgZI GCGATG 1 cut(s) 497
BtsCI GGATG 5 cut(s) 237, 481, 520, 633, 1084
BtsI GCAGTG 2 cut(s) 600, 759
BtsIMutI CAGTG 3 cut(s) 600, 759, 1027
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 6 cut(s) 162, 296, 349, 884, 973, 1152
CfoI GCGC 3 cut(s) 344, 359, 925
Cfr10I RCCGGY 2 cut(s) 91, 971
CseI GACGC 3 cut(s) 49, 290, 384
Csp6I GTAC 4 cut(s) 245, 424, 730, 742
CviAII CATG 6 cut(s) 508, 624, 866, 901, 1051, 1107
CviQI GTAC 4 cut(s) 245, 424, 730, 742
DdeI CTNAG 1 cut(s) 996
DpnI GATC 4 cut(s) 170, 236, 599, 704
DpnII GATC 4 cut(s) 168, 234, 597, 702
DraIII CACNNNGTG 2 cut(s) 265, 1064
EaeI YGGCCR 1 cut(s) 987
EciI GGCGGA 1 cut(s) 482
Ecl136II GAGCTC 2 cut(s) 12, 1056
Eco130I CCWWGG 2 cut(s) 623, 990
Eco24I GRGCYC 5 cut(s) 14, 162, 534, 1058, 1122
Eco31I GGTCTC 1 cut(s) 909
Eco47I GGWCC 2 cut(s) 442, 620
Eco53kI GAGCTC 2 cut(s) 12, 1056
Eco57I CTGAAG 2 cut(s) 398, 478
Eco72I CACGTG 1 cut(s) 354
EcoICRI GAGCTC 2 cut(s) 12, 1056
EcoO109I RGGNCCY 1 cut(s) 442
EcoRI GAATTC 1 cut(s) 946
EcoRII CCWGG 2 cut(s) 918, 1037
EcoT14I CCWWGG 2 cut(s) 623, 990
EcoT38I GRGCYC 5 cut(s) 14, 162, 534, 1058, 1122
ErhI CCWWGG 2 cut(s) 623, 990
FaeI CATG 6 cut(s) 511, 627, 869, 904, 1054, 1110
FalI AAGNNNNNCTT 2 cut(s) 548, 580
FaqI GGGAC 1 cut(s) 455
FatI CATG 6 cut(s) 507, 623, 865, 900, 1050, 1106
FauI CCCGC 2 cut(s) 169, 356
FbaI TGATCA 1 cut(s) 597
FblI GTMKAC 1 cut(s) 256
Fnu4HI GCNGC 5 cut(s) 44, 66, 130, 345, 887
FokI GGATG 5 cut(s) 244, 488, 527, 640, 1091
FriOI GRGCYC 5 cut(s) 14, 162, 534, 1058, 1122
Fsp4HI GCNGC 5 cut(s) 44, 66, 130, 345, 887
FspAI RTGCGCAY 1 cut(s) 358
FspI TGCGCA 1 cut(s) 358
GlaI GCGC 3 cut(s) 343, 358, 924
GluI GCNGC 5 cut(s) 44, 66, 130, 345, 887
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 1 cut(s) 386
HgaI GACGC 3 cut(s) 49, 290, 384
HhaI GCGC 3 cut(s) 344, 359, 925
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 6 cut(s) 511, 627, 869, 904, 1054, 1110
Hin6I GCGC 3 cut(s) 342, 357, 923
HinP1I GCGC 3 cut(s) 342, 357, 923
HinfI GANTC 6 cut(s) 209, 368, 499, 651, 710, 737
HphI GGTGA 4 cut(s) 277, 343, 617, 819
Hpy166II GTNNAC 2 cut(s) 154, 257
Hpy188I TCNGA 4 cut(s) 9, 275, 417, 650
Hpy188III TCNNGA 2 cut(s) 496, 789
Hpy8I GTNNAC 2 cut(s) 154, 257
Hpy99I CGWCG 7 cut(s) 43, 146, 200, 284, 400, 677, 710
HpyAV CCTTC 6 cut(s) 298, 422, 469, 611, 791, 862
HpyCH4III ACNGT 3 cut(s) 146, 196, 1022
HpyCH4IV ACGT 6 cut(s) 353, 378, 429, 546, 930, 959
HpyCH4V TGCA 3 cut(s) 433, 882, 1013
HpyF10VI GCNNNNNNNGC 4 cut(s) 348, 370, 464, 1160
HpyF3I CTNAG 1 cut(s) 996
HpySE526I ACGT 6 cut(s) 353, 378, 429, 546, 930, 959
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 6 cut(s) 511, 627, 869, 904, 1054, 1110
HspAI GCGC 3 cut(s) 342, 357, 923
KflI GGGWCCC 1 cut(s) 442
KroI GCCGGC 1 cut(s) 971
KroNI GCCGGC 1 cut(s) 973
Ksp22I TGATCA 1 cut(s) 597
Kzo9I GATC 4 cut(s) 168, 234, 597, 702
LmnI GCTCC 3 cut(s) 9, 17, 663
Lsp1109I GCAGC 3 cut(s) 30, 52, 898
LweI GCATC 3 cut(s) 469, 810, 906
MaeII ACGT 6 cut(s) 353, 378, 429, 546, 930, 959
MaeIII GTNAC 1 cut(s) 331
MalI GATC 4 cut(s) 170, 236, 599, 704
MboI GATC 4 cut(s) 168, 234, 597, 702
MboII GAAGA 5 cut(s) 41, 484, 568, 678, 689
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 5 cut(s) 14, 162, 534, 1058, 1122
MluCI AATT 4 cut(s) 72, 779, 946, 979
MluI ACGCGT 1 cut(s) 450
MlyI GAGTC 4 cut(s) 377, 660, 719, 731
MmeI TCCRAC 3 cut(s) 680, 738, 975
MreI CGCCGGCG 1 cut(s) 971
MroNI GCCGGC 1 cut(s) 971
MslI CAYNNNNRTG 1 cut(s) 870
MspA1I CMGCKG 2 cut(s) 177, 364
Mva1269I GAATGC 2 cut(s) 905, 1013
MvaI CCWGG 2 cut(s) 920, 1039
MvnI CGCG 3 cut(s) 263, 344, 452
MwoI GCNNNNNNNGC 4 cut(s) 348, 370, 464, 1160
NaeI GCCGGC 1 cut(s) 973
NciI CCSGG 8 cut(s) 166, 214, 504, 696, 719, 1088, 1102, 1136
NcoI CCATGG 1 cut(s) 623
NdeII GATC 4 cut(s) 168, 234, 597, 702
NgoMIV GCCGGC 1 cut(s) 971
NlaIII CATG 6 cut(s) 511, 627, 869, 904, 1054, 1110
NlaIV GGNNCC 7 cut(s) 96, 160, 170, 193, 443, 444, 532
NmeAIII GCCGAG 1 cut(s) 873
NmuCI GTSAC 1 cut(s) 331
NsbI TGCGCA 1 cut(s) 358
PaqCI CACCTGC 1 cut(s) 400
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 204
PctI GAATGC 2 cut(s) 905, 1013
PdiI GCCGGC 1 cut(s) 973
PfeI GAWTC 2 cut(s) 209, 499
PkrI GCNGC 5 cut(s) 45, 67, 131, 346, 888
PleI GAGTC 4 cut(s) 376, 659, 718, 731
PmaCI CACGTG 1 cut(s) 354
PmlI CACGTG 1 cut(s) 354
PpsI GAGTC 4 cut(s) 376, 659, 718, 731
Ppu21I YACGTR 1 cut(s) 354
PpuMI RGGWCCY 1 cut(s) 442
Psp124BI GAGCTC 2 cut(s) 14, 1058
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 2 cut(s) 918, 1037
PspCI CACGTG 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 2 cut(s) 918, 1037
PspN4I GGNNCC 7 cut(s) 96, 160, 170, 193, 443, 444, 532
PspOMI GGGCCC 2 cut(s) 158, 530
PspPPI RGGWCCY 1 cut(s) 442
PsuI RGATCY 1 cut(s) 168
PvuII CAGCTG 2 cut(s) 177, 364
RsaI GTAC 4 cut(s) 246, 425, 731, 743
RsaNI GTAC 4 cut(s) 245, 424, 730, 742
RseI CAYNNNNRTG 1 cut(s) 870
SacI GAGCTC 2 cut(s) 14, 1058
SatI GCNGC 5 cut(s) 44, 66, 130, 345, 887
Sau3AI GATC 4 cut(s) 168, 234, 597, 702
SchI GAGTC 4 cut(s) 377, 660, 719, 731
SduI GDGCHC 5 cut(s) 14, 162, 534, 1058, 1122
SfaNI GCATC 3 cut(s) 469, 810, 906
SfcI CTRYAG 1 cut(s) 1018
SgrAI CRCCGGYG 1 cut(s) 971
SinI GGWCC 2 cut(s) 442, 620
SmiMI CAYNNNNRTG 1 cut(s) 870
Sse9I AATT 4 cut(s) 72, 779, 946, 979
SsiI CCGC 8 cut(s) 129, 162, 248, 263, 344, 349, 467, 969
SstI GAGCTC 2 cut(s) 14, 1058
StyI CCWWGG 2 cut(s) 623, 990
TaaI ACNGT 3 cut(s) 146, 196, 1022
TaiI ACGT 6 cut(s) 356, 381, 432, 549, 933, 962
TaqI TCGA 6 cut(s) 198, 207, 320, 462, 672, 708
TaqII GACCGA 1 cut(s) 44
TasI AATT 4 cut(s) 72, 779, 946, 979
TatI WGTACW 1 cut(s) 423
TauI GCSGC 2 cut(s) 132, 347
TfiI GAWTC 2 cut(s) 209, 499
TscAI CASTG 3 cut(s) 600, 759, 1027
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 3 cut(s) 43, 65, 886
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 1 cut(s) 569
TspGWI ACGGA 2 cut(s) 117, 373
TspRI CASTG 3 cut(s) 600, 759, 1027
VpaK11BI GGWCC 2 cut(s) 442, 620
XapI RAATTY 3 cut(s) 72, 946, 979
XmiI GTMKAC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.