AT1G30110

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010052
Physical Location & Seq
Forward (+)
10581735 .. 10584152
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30110.2

Sequence Viewer

Length: 528 bp
ATGGAGAATCTACCACCTGGTTATCGTCCCAATGTTGGTGTTTGTCTAATCAACTCGGATAATCTGGTATTTGTAGCTTCTAGATTGAATGTTCCAGGAGCATGGCAGATGCCACAGGGAGGCATTGAAGATGGGGAGGATCCAAAGTCAGCAGCCATGAGAGAGTTACAAGAAGAAACTGGTGTTGTTTCAGCTGAAATCGTCTCTGAGGTCCCAAATTGGTTGACATATGATTTTCCACCAGCAGTAAAAGCAAAAGTTAACCGTCTTTGGGGCGGTGAATGGCATGGTCAAGCTCAGAAATGGTATTTAGTGAGACTGAGGAACGATGAGGACGAGAAAGAGATCAATCTAGCGAACAACGAAGCGGATTCAGAGTTTGCGGAGTGGAAATGGGCGAAGCCAGAAGAAGTGGTAGAGCAAGCAGTGGATTACAAAAGGCCAACCTATGAAGAAGTCATCAAGACTTTTGGTTCGTTCTTAAACGACACAGGAAGAGCTGCTAAATGTAAATCAGCCAAGTGGTGA

Protein Analysis

175

Amino Acids

19.83

Weight (kDa)

4.76

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 8 - 150 3.6e-23 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30110 AT1G30110
fragaria_vesca FvH4_4g23240 FvH4_4g32510 FvH4_4g32510 FvH4_4g32510
malus_domestica MD13G1024600.v1.1 MD16G1027300.v1.1
prunus_persica Prupe.1G325900_v2.0.a1
pyrus_communis pycom13g02290 pycom16g02310
rosa_chinensis RchiOBHm_Chr4g0441391
rosa_laevigata RLG00000006095
rosa_multiflora Rmu_sc0005516.1_g000004
rosa_roxburghii Rroxscaffold_5G00382270
rosa_rugosa Rorug04G0333000
rosa_samantha Rh4AG385000 Rh4BG399000 Rh4CG414100 Rh4DG391700
rosa_wichuraiana Rw4G033240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 276, 368, 383
AclWI GGATC 2 cut(s) 134, 147
AfiI CCNNNNNNNGG 3 cut(s) 35, 119, 271
AgsI TTSAA 2 cut(s) 88, 128
AjnI CCWGG 2 cut(s) 16, 94
AluBI AGCT 4 cut(s) 77, 194, 296, 500
AluI AGCT 4 cut(s) 77, 194, 296, 500
Alw26I GTCTC 2 cut(s) 208, 310
AlwI GGATC 2 cut(s) 134, 147
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 2 cut(s) 152, 500
ArsI GACNNNNNNTTYG 2 cut(s) 457, 489
AspS9I GGNCC 1 cut(s) 211
AsuHPI GGTGA 1 cut(s) 290
AvaII GGWCC 1 cut(s) 211
BamHI GGATCC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 164, 487
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 2 cut(s) 18, 96
BcoDI GTCTC 2 cut(s) 208, 310
BfaI CTAG 2 cut(s) 81, 353
BisI GCNGC 2 cut(s) 153, 501
BlsI GCNGC 2 cut(s) 154, 502
Bme1390I CCNGG 2 cut(s) 18, 96
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 141, 213
BmrFI CCNGG 2 cut(s) 18, 96
BmsI GCATC 1 cut(s) 99
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 119, 271
Bse1I ACTGG 1 cut(s) 184
BseBI CCWGG 2 cut(s) 18, 96
BseLI CCNNNNNNNGG 3 cut(s) 35, 119, 271
BseMII CTCAG 3 cut(s) 198, 311, 311
BseNI ACTGG 1 cut(s) 184
BseXI GCAGC 2 cut(s) 164, 487
BshFI GGCC 1 cut(s) 442
BslFI GGGAC 2 cut(s) 12, 197
BslI CCNNNNNNNGG 3 cut(s) 35, 119, 271
BsmAI GTCTC 2 cut(s) 208, 310
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 2 cut(s) 12, 197
BsnI GGCC 1 cut(s) 442
Bsp143I GATC 2 cut(s) 139, 345
BspACI CCGC 3 cut(s) 276, 368, 383
BspANI GGCC 1 cut(s) 442
BspCNI CTCAG 3 cut(s) 199, 310, 312
BspLI GGNNCC 2 cut(s) 141, 213
BspPI GGATC 2 cut(s) 134, 147
BspQI GCTCTTC 1 cut(s) 490
BsrI ACTGG 1 cut(s) 184
BssMI GATC 2 cut(s) 139, 345
Bst2UI CCWGG 2 cut(s) 18, 96
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 1 cut(s) 490
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 3 cut(s) 207, 297, 320
BstKTI GATC 2 cut(s) 142, 348
BstMAI GTCTC 2 cut(s) 208, 310
BstMBI GATC 2 cut(s) 139, 345
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 2 cut(s) 18, 96
BstSCI CCNGG 2 cut(s) 16, 94
BstV1I GCAGC 2 cut(s) 164, 487
BstX2I RGATCY 1 cut(s) 139
BstXI CCANNNNNNTGG 1 cut(s) 102
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 1 cut(s) 442
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 1 cut(s) 432
Cac8I GCNNGC 1 cut(s) 423
Cfr13I GGNCC 1 cut(s) 211
CsiI ACCWGGT 1 cut(s) 16
CviAII CATG 3 cut(s) 102, 157, 287
CviJI RGCY 8 cut(s) 77, 155, 194, 296, 403, 442, 500, 518
CviKI_1 RGCY 8 cut(s) 77, 155, 194, 296, 403, 442, 500, 518
DdeI CTNAG 3 cut(s) 207, 297, 320
DpnI GATC 2 cut(s) 141, 347
DpnII GATC 2 cut(s) 139, 345
Eam1104I CTCTTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 211
EcoRII CCWGG 2 cut(s) 16, 94
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 3 cut(s) 105, 160, 290
FaiI YATR 6 cut(s) 103, 158, 229, 231, 288, 450
FaqI GGGAC 2 cut(s) 12, 197
FatI CATG 3 cut(s) 101, 156, 286
FauNDI CATATG 1 cut(s) 229
Fnu4HI GCNGC 2 cut(s) 153, 501
Fsp4HI GCNGC 2 cut(s) 153, 501
FspBI CTAG 2 cut(s) 81, 353
GluI GCNGC 2 cut(s) 153, 501
HaeIII GGCC 1 cut(s) 442
Hin1II CATG 3 cut(s) 105, 160, 290
HincII GTYRAC 2 cut(s) 225, 262
HindII GTYRAC 2 cut(s) 225, 262
HinfI GANTC 2 cut(s) 7, 371
HpaI GTTAAC 1 cut(s) 262
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 2 cut(s) 225, 262
Hpy188I TCNGA 4 cut(s) 58, 208, 300, 376
Hpy188III TCNNGA 2 cut(s) 81, 463
Hpy8I GTNNAC 2 cut(s) 225, 262
HpyCH4III ACNGT 1 cut(s) 266
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 3 cut(s) 207, 297, 320
Hsp92II CATG 3 cut(s) 105, 160, 290
KspAI GTTAAC 1 cut(s) 262
Kzo9I GATC 2 cut(s) 139, 345
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 1 cut(s) 98
Lsp1109I GCAGC 2 cut(s) 164, 487
LweI GCATC 1 cut(s) 99
MabI ACCWGGT 1 cut(s) 16
MaeI CTAG 2 cut(s) 81, 353
MaeIII GTNAC 1 cut(s) 165
MalI GATC 2 cut(s) 141, 347
MboI GATC 2 cut(s) 139, 345
MboII GAAGA 5 cut(s) 140, 185, 419, 464, 507
MflI RGATCY 1 cut(s) 139
MluCI AATT 1 cut(s) 217
MnlI CCTC 5 cut(s) 113, 130, 202, 315, 325
MseI TTAA 2 cut(s) 261, 482
MspA1I CMGCKG 1 cut(s) 194
MspR9I CCNGG 2 cut(s) 18, 96
MvaI CCWGG 2 cut(s) 18, 96
MwoI GCNNNNNNNGC 1 cut(s) 251
NdeI CATATG 1 cut(s) 229
NdeII GATC 2 cut(s) 139, 345
NlaIII CATG 3 cut(s) 105, 160, 290
NlaIV GGNNCC 2 cut(s) 141, 213
PciSI GCTCTTC 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PfeI GAWTC 2 cut(s) 7, 371
PfoI TCCNGGA 1 cut(s) 94
PkrI GCNGC 2 cut(s) 154, 502
PpuMI RGGWCCY 1 cut(s) 211
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 2 cut(s) 16, 94
PspGI CCWGG 2 cut(s) 16, 94
PspN4I GGNNCC 2 cut(s) 141, 213
PspPI GGNCC 1 cut(s) 211
PspPPI RGGWCCY 1 cut(s) 211
PsuI RGATCY 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 2 cut(s) 261, 482
SatI GCNGC 2 cut(s) 153, 501
Sau3AI GATC 2 cut(s) 139, 345
Sau96I GGNCC 1 cut(s) 211
ScrFI CCNGG 2 cut(s) 18, 96
SetI ASST 7 cut(s) 19, 79, 196, 213, 298, 449, 502
SexAI ACCWGGT 1 cut(s) 16
SfaNI GCATC 1 cut(s) 99
SinI GGWCC 1 cut(s) 211
Sse9I AATT 1 cut(s) 217
SsiI CCGC 3 cut(s) 276, 368, 383
SspMI CTAG 2 cut(s) 81, 353
StyD4I CCNGG 2 cut(s) 16, 94
TaaI ACNGT 1 cut(s) 266
TasI AATT 1 cut(s) 217
TfiI GAWTC 2 cut(s) 7, 371
Tru1I TTAA 2 cut(s) 261, 482
Tru9I TTAA 2 cut(s) 261, 482
TscAI CASTG 1 cut(s) 432
TseI GCWGC 2 cut(s) 152, 500
TspDTI ATGAA 1 cut(s) 465
TspRI CASTG 1 cut(s) 432
VpaK11BI GGWCC 1 cut(s) 211
XbaI TCTAGA 1 cut(s) 80
XspI CTAG 2 cut(s) 81, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.