Rh4AG385000

Belongs to the Nudix hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0010052
Physical Location & Seq
Reverse (-)
69098751 .. 69101175
2425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG385000.1

Sequence Viewer

Length: 522 bp
ATGGAGGATCTGCCCTCCGGTTACCGTCCCAACGTCGGCGTTTGCCTCATTAACTCCGATAATCTGATTTTTGTTGGATCAAGATTGAATGTTCCCGGAGCATGGCAGATGCCTCAGGGCGGTATTGAAGATGGCGAAGAACCCAAATCAGCAGCTGTTAGGGAACTACGAGAAGAGACTGGGGTAGTGTCTGCTGAAATTATAGCTGAGGTTCCAAATTGGTTGACATATGACTTCCCACCTGCCGTGAAGACCAAAGTTAATCGTCTCTGGGGAGGTGAATGGCATGGGCAAGCACAAAAATGGTTTCTTATGAGATTCACAAATGATGAGAGTGAGATCAACTTAGCTACTGGGGAAGCTGATGCAGAGTTTGCAGAGTGGAAATGGGCGAGCCCTGAAGAAGTTATCGAGCAGGCAGTGGACTACAAGAGGCCAACATATGAGGAAGTTATGAAGACCTTCAAGTCGTACTTGGACGGAAGTGGATTATCTGCAAAATGTAAATCAACAAAATGGTGA

Protein Analysis

173

Amino Acids

19.49

Weight (kDa)

4.68

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 8 - 149 3e-23 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30110 AT1G30110
fragaria_vesca FvH4_4g23240 FvH4_4g32510 FvH4_4g32510 FvH4_4g32510
malus_domestica MD13G1024600.v1.1 MD16G1027300.v1.1
prunus_persica Prupe.1G325900_v2.0.a1
pyrus_communis pycom13g02290 pycom16g02310
rosa_chinensis RchiOBHm_Chr4g0441391
rosa_laevigata RLG00000006095
rosa_multiflora Rmu_sc0005516.1_g000004
rosa_roxburghii Rroxscaffold_5G00382270
rosa_rugosa Rorug04G0333000
rosa_samantha Rh4AG385000 Rh4BG399000 Rh4CG414100 Rh4DG391700
rosa_wichuraiana Rw4G033240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 250
AasI GACNNNNNNGTC 1 cut(s) 466
Acc36I ACCTGC 1 cut(s) 250
AciI CCGC 1 cut(s) 120
AclWI GGATC 2 cut(s) 15, 85
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 3 cut(s) 35, 102, 119
AgsI TTSAA 3 cut(s) 88, 128, 466
AluBI AGCT 4 cut(s) 155, 206, 350, 362
AluI AGCT 4 cut(s) 155, 206, 350, 362
Alw26I GTCTC 2 cut(s) 170, 272
AlwI GGATC 2 cut(s) 15, 85
AlwNI CAGNNNCTG 1 cut(s) 155
AoxI GGCC 1 cut(s) 434
ApeKI GCWGC 1 cut(s) 152
Asp700I GAANNNNTTC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 290
AxyI CCTNAGG 1 cut(s) 114
BanII GRGCYC 1 cut(s) 398
BbsI GAAGAC 2 cut(s) 257, 464
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 164
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 230
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 2 cut(s) 170, 272
BfuAI ACCTGC 1 cut(s) 250
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme1390I CCNGG 1 cut(s) 96
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 1 cut(s) 96
BmrI ACTGGG 2 cut(s) 189, 363
BmsI GCATC 2 cut(s) 99, 355
BmuI ACTGGG 2 cut(s) 189, 363
BpiI GAAGAC 2 cut(s) 257, 464
Bpu10I CCTNAGC 1 cut(s) 207
BpuMI CCSGG 1 cut(s) 96
BsaWI WCCGGW 1 cut(s) 17
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 102, 119
Bse1I ACTGG 2 cut(s) 184, 358
Bse21I CCTNAGG 1 cut(s) 114
BseLI CCNNNNNNNGG 3 cut(s) 35, 102, 119
BseMII CTCAG 2 cut(s) 128, 198
BseNI ACTGG 2 cut(s) 184, 358
BseXI GCAGC 1 cut(s) 164
BshFI GGCC 1 cut(s) 436
BsiSI CCGG 2 cut(s) 18, 96
BslFI GGGAC 1 cut(s) 12
BslI CCNNNNNNNGG 3 cut(s) 35, 102, 119
BsmAI GTCTC 2 cut(s) 170, 272
BsmBI CGTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 12
BsnI GGCC 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 398
Bsp143I GATC 3 cut(s) 7, 77, 339
BspACI CCGC 1 cut(s) 120
BspANI GGCC 1 cut(s) 436
BspCNI CTCAG 2 cut(s) 127, 199
BspLI GGNNCC 1 cut(s) 213
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 2 cut(s) 15, 85
BsrI ACTGG 2 cut(s) 184, 358
BssMI GATC 3 cut(s) 7, 77, 339
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 3 cut(s) 294, 394, 417
BstDEI CTNAG 3 cut(s) 114, 207, 346
BstEII GGTNACC 1 cut(s) 20
BstKTI GATC 3 cut(s) 10, 80, 342
BstMAI GTCTC 2 cut(s) 170, 272
BstMBI GATC 3 cut(s) 7, 77, 339
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstPI GGTNACC 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 164
BstV2I GAAGAC 2 cut(s) 257, 464
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
Bsu36I CCTNAGG 1 cut(s) 114
BsuRI GGCC 1 cut(s) 436
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 3 cut(s) 294, 394, 417
CaiI CAGNNNCTG 1 cut(s) 155
Csp6I GTAC 1 cut(s) 472
CviAII CATG 2 cut(s) 102, 287
CviJI RGCY 6 cut(s) 155, 206, 350, 362, 396, 436
CviKI_1 RGCY 6 cut(s) 155, 206, 350, 362, 396, 436
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 3 cut(s) 114, 207, 346
DpnI GATC 3 cut(s) 9, 79, 341
DpnII GATC 3 cut(s) 7, 77, 339
DrdI GACNNNNNNGTC 1 cut(s) 466
DseDI GACNNNNNNGTC 1 cut(s) 466
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 398
Eco57I CTGAAG 1 cut(s) 420
Eco81I CCTNAGG 1 cut(s) 114
Eco91I GGTNACC 1 cut(s) 20
EcoO65I GGTNACC 1 cut(s) 20
EcoT38I GRGCYC 1 cut(s) 398
Esp3I CGTCTC 1 cut(s) 272
FaeI CATG 2 cut(s) 105, 290
FaiI YATR 9 cut(s) 103, 203, 229, 231, 288, 314, 442, 444, 455
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 1 cut(s) 12
FatI CATG 2 cut(s) 101, 286
FauNDI CATATG 2 cut(s) 229, 442
Fnu4HI GCNGC 1 cut(s) 153
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 1 cut(s) 153
GluI GCNGC 1 cut(s) 153
HaeIII GGCC 1 cut(s) 436
HapII CCGG 2 cut(s) 18, 96
Hin1II CATG 2 cut(s) 105, 290
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 1 cut(s) 318
HpaII CCGG 2 cut(s) 18, 96
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 2 cut(s) 225, 424
Hpy188I TCNGA 2 cut(s) 58, 66
Hpy188III TCNNGA 1 cut(s) 81
Hpy8I GTNNAC 2 cut(s) 225, 424
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 1 cut(s) 472
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 3 cut(s) 368, 377, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 3 cut(s) 114, 207, 346
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 2 cut(s) 105, 290
Kzo9I GATC 3 cut(s) 7, 77, 339
LmnI GCTCC 1 cut(s) 98
LpnPI CCDG 9 cut(s) 31, 101, 109, 165, 255, 256, 339, 401, 411
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 2 cut(s) 99, 355
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 20
MalI GATC 3 cut(s) 9, 79, 341
MboI GATC 3 cut(s) 7, 77, 339
MboII GAAGA 6 cut(s) 140, 149, 185, 262, 413, 469
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 398
MluCI AATT 2 cut(s) 198, 217
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 7 cut(s) 25, 56, 123, 202, 269, 426, 439
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 2 cut(s) 51, 261
MslI CAYNNNNRTG 1 cut(s) 301
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 2 cut(s) 18, 96
MspR9I CCNGG 1 cut(s) 96
MwoI GCNNNNNNNGC 1 cut(s) 374
NciI CCSGG 1 cut(s) 96
NdeI CATATG 2 cut(s) 229, 442
NdeII GATC 3 cut(s) 7, 77, 339
NlaIII CATG 2 cut(s) 105, 290
NlaIV GGNNCC 1 cut(s) 213
PaqCI CACCTGC 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 1 cut(s) 318
PfoI TCCNGGA 1 cut(s) 94
PkrI GCNGC 1 cut(s) 154
PspEI GGTNACC 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 213
PstNI CAGNNNCTG 1 cut(s) 155
PsuI RGATCY 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
RseI CAYNNNNRTG 1 cut(s) 301
SaqAI TTAA 2 cut(s) 51, 261
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 3 cut(s) 7, 77, 339
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 1 cut(s) 398
SetI ASST 9 cut(s) 36, 157, 208, 213, 244, 280, 352, 364, 464
SfaNI GCATC 2 cut(s) 99, 355
SmiMI CAYNNNNRTG 1 cut(s) 301
Sse9I AATT 2 cut(s) 198, 217
SsiI CCGC 1 cut(s) 120
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 26
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 411
TasI AATT 2 cut(s) 198, 217
TfiI GAWTC 1 cut(s) 318
Tru1I TTAA 2 cut(s) 51, 261
Tru9I TTAA 2 cut(s) 51, 261
TscAI CASTG 1 cut(s) 426
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 1 cut(s) 470
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.