AT1G31540

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002912
Physical Location & Seq
Reverse (-)
11288378 .. 11293799
5422 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G31540.3

Sequence Viewer

Length: 2331 bp
ATGGCTTCGTCATCTTCTTCTCACAATTGGTTATATGATGTTTTCTTGAGCTTCAGAGGGGAAGACGTCCGCGTAACATTCCGTAGCCACTTTCTCAAAGAGCTTGATCGGAAACTAATCACTGCTTTCAGAGACAATGAGATCGAGAGAAGCCACTCTCTCTGGCCCGATCTTGAACAAGCCATCAAGGAATCCAGGATCGCTGTGGTCGTTTTCTCAAAAAACTACGCTTCATCGAGCTGGTGTCTTAACGAGTTGCTGGAGATTGTAAACTGCAATGATAAAATTGTCATACCAGTTTTCTACCATGTGGATCCTTCCCAAGTGAGGCATCAAATCGGCGACTTCGGAAAGATCTTTGAAAATACTTGCAAGAGACAAACAGATGAGGAAGTGAAAAATCAATGGAAGAAAGCGTTGACTCTTGTAGCGAATATGCTTGGATTTGACTCTGCCAAATGGAACGACGAAGCAAAAATGATTGAAGAAATAGCCAATGATGTTTTGGGTAAACTGCTTTTAACTACACCCAAGGATTCTGAGGAACTTGTTGGCATCGAAGATCACATCGCTGAAATGAGTTTACTGCTGCAACTGGAATCTAAAGAAGTGAGAATGGTTGGTATATCGGGTTCCTCAGGGATTGGTAAGACTACCATTGCAAGAGCTCTGTTTAAACGACTTTCTCGACATTTCCAAGGTAGCACTTTCATCGACAGGGCTTTTGTATCTTATAGTCGGAATATTTATAGTGGCGCCAATCCGGACGACCCCAATATGAAGTTGCAGCTACAAGGACACTTCCTATCTGAAATTCTCGGGAAAAAAGACATAAAGATAGATGATCCAGCTGCATTGGAAGAGAGGCTAAAGCACCAAAAAGTTCTTATCATTATTGATGATTTGGATGATATAATGGTACTAGATACATTAGTGGGTCAAACTCAATGGTTTGGATATGGGAGCAGAATCATTGTGGTTACAAATGATAAGCACTTCTTGATTGCTCATGGAATTGATCATATTTATGAAGTAAGTTTCCCAACTGATGTGCATGCTTGTCAGATGTTATGTCAATCTGCATTCAAGCAAAACTATGCTCCTAAAGGTTTTGAGGATCTTGTAGTTGATGTAGTAAGACATGCGGGCAATTTTCCTTTGGGTCTTAATCTTTTGGGTAAATATTTGCGGAGGAGGGATATGGAATACTGGATGGATATGCTGCCAAGGCTTGAGAATAGTCTACGTATAGACGGTAAAATTGAAAAAATACTAAGAATCAGCTATGATGGGTTAGAGAGTGAAGATCAAGAGATATTTCGTCATATCGCATGTCTTTTCAATCATATGGAAGTCACAACCATCAAGTCCTTGCTGGCAGATAGTGATGTTAGTTTTGCACTAGAAAACCTAGCTGATAAGTCCCTCATTCATGTCAGACAAGGTTATGTGGTGATGCACCGTTCGCTACAAGAAATGGGTAGAAAAATTGTTCGCATTCAGTCCATTGACAAACCTGGAGAACGAGAATTTCTGGTGGATCCAAATGATATACATGATATACTCAATGCATGCACTGGTACTCAAAAGGTTTTAGGTATATCACTGGATATAAGGAATATTCGTGAGTTGGATGTACATGAGAGGGCCTTCAAAGGGATGTCTAATCTCCGTTTCTTAGAAATTAAGAACTTTGGTTTGAAAGAAGACGGTTTGCATTTACCTCCAAGTTTCGACTATTTGCCCCGTACACTCAAACTATTGTGCTGGTCCAAATTTCCAATGAGGTGTATGCCTTTTGGTTTTCGTCCTGAAAACCTTGTCAAGCTCGAAATGCAGTACAGCAAGCTACATAAGCTATGGGAAGGAGTTGCGCCACTTACATGTCTAAAGGAAATGGATCTGCACGGATCATCAAACCTGAAAGTAATTCCAGATCTTTCTGAAGCTACTAATCTCGAGATACTTAATCTTAAGTTTTGCGAGAGTTTGGTCGAGCTCCCGTCCTCTATACGAAATCTCAATAAACTGTTGAACTTGGACATGCTAAACTGCAAAAGTCTGAAAATTCTTCCTACCGGATTCAACCTCAAATCTCTTGACCGCCTCAATCTCTATCATTGCTCAAAGTTGAAGACTTTTCCCAAATTCTCAACCAACATCTCAGTTCTCAATCTAAATCTAACAAACATTGAAGATTTCCCTTCTAATTTACATCTCGAGAATCTTGTTGAGTTTAGAATATCAAAAGAAGAGAGTGATGAGAAACAATGGGAAGAAGAGAAGGTAAGTAGTTCTACACTTAACATTATCTCCAAACTCTTTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

776

Amino Acids

89.12

Weight (kDa)

6.07

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 186 1.8e-55 TIR domain
TIR_2 PF13676 14 - 108 4.4e-12 TIR domain
AAA_16 PF13191 183 - 304 3.5e-07 AAA ATPase domain
NB-ARC PF00931 199 - 365 1.7e-09 NB-ARC domain
WHD_ROQ1 PF23282 434 - 502 3.7e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 606 - 624 5.2e-09 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 69
AccB1I GGYRCC 1 cut(s) 755
AccI GTMKAC 1 cut(s) 1243
AccII CGCG 1 cut(s) 72
AccIII TCCGGA 1 cut(s) 763
AciI CCGC 4 cut(s) 70, 1145, 1189, 2104
AclWI GGATC 9 cut(s) 206, 308, 321, 839, 1125, 1535, 1548, 1908, 1918
AcsI RAATTY 5 cut(s) 813, 1529, 1775, 2067, 2147
AcuI CTGAAG 2 cut(s) 37, 1965
AcyI GRCGYC 2 cut(s) 66, 756
AfaI GTAC 5 cut(s) 921, 1582, 1638, 1750, 1841
AfiI CCNNNNNNNGG 2 cut(s) 327, 1656
AflII CTTAAG 1 cut(s) 1973
AflIII ACRYGT 1 cut(s) 1883
AjnI CCWGG 2 cut(s) 194, 1516
Alw21I GWGCWC 2 cut(s) 670, 2001
Alw26I GTCTC 2 cut(s) 126, 370
AlwI GGATC 9 cut(s) 206, 308, 321, 839, 1125, 1535, 1548, 1908, 1918
Ama87I CYCGRG 3 cut(s) 818, 1958, 2219
Aor13HI TCCGGA 1 cut(s) 763
AoxI GGCC 2 cut(s) 164, 1647
ApeKI GCWGC 4 cut(s) 589, 787, 851, 1222
ApoI RAATTY 5 cut(s) 813, 1529, 1775, 2067, 2147
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
AspLEI GCGC 2 cut(s) 758, 1876
AspS9I GGNCC 3 cut(s) 165, 1647, 1770
AsuHPI GGTGA 1 cut(s) 1465
AvaI CYCGRG 3 cut(s) 818, 1958, 2219
AvaII GGWCC 1 cut(s) 1770
AxyI CCTNAGG 1 cut(s) 637
BaeI ACNNNNGTAYC 2 cut(s) 918, 951
BamHI GGATCC 2 cut(s) 313, 1540
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 2 cut(s) 670, 2001
BbsI GAAGAC 3 cut(s) 69, 1713, 2141
Bbv12I GWGCWC 2 cut(s) 670, 2001
BbvI GCAGC 4 cut(s) 576, 799, 838, 1209
BccI CCATC 4 cut(s) 191, 1207, 1283, 1370
BcgI CGANNNNNNTGC 2 cut(s) 694, 728
BciT130I CCWGG 2 cut(s) 196, 1518
BclI TGATCA 1 cut(s) 1018
BcoDI GTCTC 2 cut(s) 126, 370
BfaI CTAG 3 cut(s) 923, 1403, 1412
BfoI RGCGCY 1 cut(s) 759
BfrI CTTAAG 1 cut(s) 1973
BglII AGATCT 2 cut(s) 354, 1936
BisI GCNGC 4 cut(s) 590, 788, 852, 1223
BlsI GCNGC 4 cut(s) 591, 789, 853, 1224
Bme1390I CCNGG 2 cut(s) 196, 1518
Bme18I GGWCC 1 cut(s) 1770
BmeT110I CYCGRG 3 cut(s) 818, 1958, 2219
BmgT120I GGNCC 3 cut(s) 165, 1647, 1770
BmiI GGNNCC 4 cut(s) 315, 634, 757, 1542
BmrFI CCNGG 2 cut(s) 196, 1518
BmsI GCATC 3 cut(s) 340, 564, 1446
BpiI GAAGAC 3 cut(s) 69, 1713, 2141
BpmI CTGGAG 2 cut(s) 281, 1539
BpuEI CTTGAG 2 cut(s) 67, 1253
BsaAI YACGTR 1 cut(s) 1247
BsaHI GRCGYC 2 cut(s) 66, 756
BsaJI CCNNGG 3 cut(s) 531, 697, 1226
BsaWI WCCGGW 2 cut(s) 763, 2078
BsaXI ACNNNNNCTCC 2 cut(s) 2297, 2327
Bsc4I CCNNNNNNNGG 2 cut(s) 327, 1656
Bse1I ACTGG 5 cut(s) 296, 600, 1214, 1582, 1611
Bse21I CCTNAGG 1 cut(s) 637
Bse3DI GCAATG 3 cut(s) 283, 657, 2119
BseAI TCCGGA 1 cut(s) 763
BseBI CCWGG 2 cut(s) 196, 1518
BseDI CCNNGG 3 cut(s) 531, 697, 1226
BseGI GGATG 4 cut(s) 913, 1218, 1639, 1665
BseLI CCNNNNNNNGG 2 cut(s) 327, 1656
BseMI GCAATG 3 cut(s) 283, 657, 2119
BseMII CTCAG 3 cut(s) 531, 651, 2178
BseNI ACTGG 5 cut(s) 296, 600, 1214, 1582, 1611
BseRI GAGGAG 1 cut(s) 1207
BseXI GCAGC 4 cut(s) 576, 799, 838, 1209
BsgI GTGCAG 1 cut(s) 1889
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 2 cut(s) 166, 1649
BshNI GGYRCC 1 cut(s) 755
BsiHKAI GWGCWC 2 cut(s) 670, 2001
BsiHKCI CYCGRG 3 cut(s) 818, 1958, 2219
BsiSI CCGG 2 cut(s) 764, 2079
BslFI GGGAC 1 cut(s) 1408
BslI CCNNNNNNNGG 2 cut(s) 327, 1656
BsmAI GTCTC 2 cut(s) 126, 370
BsmFI GGGAC 1 cut(s) 1408
BsmI GAATGC 2 cut(s) 1082, 1497
BsnI GGCC 2 cut(s) 166, 1649
BsoBI CYCGRG 3 cut(s) 818, 1958, 2219
Bsp1286I GDGCHC 2 cut(s) 670, 2001
Bsp13I TCCGGA 1 cut(s) 763
Bsp1407I TGTACA 1 cut(s) 1636
BspACI CCGC 4 cut(s) 70, 1145, 1189, 2104
BspANI GGCC 2 cut(s) 166, 1649
BspCNI CTCAG 3 cut(s) 532, 650, 2177
BspEI TCCGGA 1 cut(s) 763
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 4 cut(s) 315, 634, 757, 1542
BspPI GGATC 9 cut(s) 206, 308, 321, 839, 1125, 1535, 1548, 1908, 1918
BspT107I GGYRCC 1 cut(s) 755
BspTI CTTAAG 1 cut(s) 1973
BsrDI GCAATG 3 cut(s) 283, 657, 2119
BsrGI TGTACA 1 cut(s) 1636
BsrI ACTGG 5 cut(s) 296, 600, 1214, 1582, 1611
BssECI CCNNGG 3 cut(s) 531, 697, 1226
BssNI GRCGYC 2 cut(s) 66, 756
BssT1I CCWWGG 3 cut(s) 531, 697, 1226
Bst2UI CCWGG 2 cut(s) 196, 1518
Bst4CI ACNGT 4 cut(s) 1256, 1463, 1712, 2031
Bst6I CTCTTC 3 cut(s) 855, 2247, 2274
BstACI GRCGYC 2 cut(s) 66, 756
BstAFI CTTAAG 1 cut(s) 1973
BstAUI TGTACA 1 cut(s) 1636
BstBAI YACGTR 1 cut(s) 1247
BstC8I GCNNGC 5 cut(s) 1056, 1147, 1377, 1573, 1847
BstDEI CTNAG 5 cut(s) 540, 637, 1274, 1678, 2164
BstF5I GGATG 4 cut(s) 913, 1218, 1639, 1665
BstFNI CGCG 1 cut(s) 72
BstH2I RGCGCY 1 cut(s) 759
BstHHI GCGC 2 cut(s) 758, 1876
BstMAI GTCTC 2 cut(s) 126, 370
BstMWI GCNNNNNNNGC 4 cut(s) 1228, 1465, 1834, 1855
BstNI CCWGG 2 cut(s) 196, 1518
BstNSI RCATGY 6 cut(s) 1058, 1145, 1335, 1575, 1887, 2047
BstSCI CCNGG 2 cut(s) 194, 1516
BstSNI TACGTA 1 cut(s) 1247
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 4 cut(s) 576, 799, 838, 1209
BstV2I GAAGAC 3 cut(s) 69, 1713, 2141
BstX2I RGATCY 6 cut(s) 313, 354, 1117, 1540, 1900, 1936
BstYI RGATCY 6 cut(s) 313, 354, 1117, 1540, 1900, 1936
Bsu36I CCTNAGG 1 cut(s) 637
BsuRI GGCC 2 cut(s) 166, 1649
BtgZI GCGATG 1 cut(s) 553
BtsCI GGATG 4 cut(s) 913, 1218, 1639, 1665
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 3 cut(s) 120, 1575, 1604
Cac8I GCNNGC 5 cut(s) 1056, 1147, 1377, 1573, 1847
CfoI GCGC 2 cut(s) 758, 1876
Cfr13I GGNCC 3 cut(s) 165, 1647, 1770
Csp6I GTAC 5 cut(s) 920, 1581, 1637, 1749, 1840
CviQI GTAC 5 cut(s) 920, 1581, 1637, 1749, 1840
DdeI CTNAG 5 cut(s) 540, 637, 1274, 1678, 2164
DinI GGCGCC 1 cut(s) 757
DraI TTTAAA 1 cut(s) 676
Eam1104I CTCTTC 3 cut(s) 855, 2247, 2274
EarI CTCTTC 3 cut(s) 855, 2247, 2274
Ecl136II GAGCTC 2 cut(s) 668, 1999
Eco105I TACGTA 1 cut(s) 1247
Eco130I CCWWGG 3 cut(s) 531, 697, 1226
Eco24I GRGCYC 2 cut(s) 670, 2001
Eco47I GGWCC 1 cut(s) 1770
Eco53kI GAGCTC 2 cut(s) 668, 1999
Eco57I CTGAAG 2 cut(s) 37, 1965
Eco81I CCTNAGG 1 cut(s) 637
Eco88I CYCGRG 3 cut(s) 818, 1958, 2219
EcoICRI GAGCTC 2 cut(s) 668, 1999
EcoO109I RGGNCCY 1 cut(s) 1647
EcoRII CCWGG 2 cut(s) 194, 1516
EcoT14I CCWWGG 3 cut(s) 531, 697, 1226
EcoT22I ATGCAT 1 cut(s) 1573
EcoT38I GRGCYC 2 cut(s) 670, 2001
EgeI GGCGCC 1 cut(s) 757
EheI GGCGCC 1 cut(s) 757
ErhI CCWWGG 3 cut(s) 531, 697, 1226
FalI AAGNNNNNCTT 2 cut(s) 983, 1015
FaqI GGGAC 1 cut(s) 1408
FauI CCCGC 1 cut(s) 1138
FauNDI CATATG 1 cut(s) 1347
FbaI TGATCA 1 cut(s) 1018
FblI GTMKAC 1 cut(s) 1243
Fnu4HI GCNGC 4 cut(s) 590, 788, 852, 1223
FokI GGATG 4 cut(s) 920, 1225, 1646, 1672
FriOI GRGCYC 2 cut(s) 670, 2001
Fsp4HI GCNGC 4 cut(s) 590, 788, 852, 1223
FspBI CTAG 3 cut(s) 923, 1403, 1412
GlaI GCGC 2 cut(s) 757, 1875
GluI GCNGC 4 cut(s) 590, 788, 852, 1223
GsuI CTGGAG 2 cut(s) 281, 1539
HaeII RGCGCY 1 cut(s) 759
HaeIII GGCC 2 cut(s) 166, 1649
HapII CCGG 2 cut(s) 764, 2079
HhaI GCGC 2 cut(s) 758, 1876
Hin1I GRCGYC 2 cut(s) 66, 756
Hin6I GCGC 2 cut(s) 756, 1874
HinP1I GCGC 2 cut(s) 756, 1874
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HinfI GANTC 9 cut(s) 191, 421, 449, 536, 599, 969, 1278, 2082, 2224
HpaII CCGG 2 cut(s) 764, 2079
HphI GGTGA 1 cut(s) 1465
Hpy166II GTNNAC 6 cut(s) 271, 420, 512, 584, 1244, 1751
Hpy8I GTNNAC 6 cut(s) 271, 420, 512, 584, 1244, 1751
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 5 cut(s) 327, 1660, 1859, 2214, 2278
HpyCH4III ACNGT 4 cut(s) 1256, 1463, 1712, 2031
HpyCH4IV ACGT 2 cut(s) 66, 1246
HpyF10VI GCNNNNNNNGC 4 cut(s) 1228, 1465, 1834, 1855
HpyF3I CTNAG 5 cut(s) 540, 637, 1274, 1678, 2164
HpySE526I ACGT 2 cut(s) 66, 1246
Hsp92I GRCGYC 2 cut(s) 66, 756
HspAI GCGC 2 cut(s) 756, 1874
KasI GGCGCC 1 cut(s) 755
Kpn2I TCCGGA 1 cut(s) 763
Ksp22I TGATCA 1 cut(s) 1018
LmnI GCTCC 3 cut(s) 963, 1105, 2004
Lsp1109I GCAGC 4 cut(s) 576, 799, 838, 1209
LweI GCATC 3 cut(s) 340, 564, 1446
MaeI CTAG 3 cut(s) 923, 1403, 1412
MaeII ACGT 2 cut(s) 66, 1246
MaeIII GTNAC 3 cut(s) 73, 979, 1354
MfeI CAATTG 1 cut(s) 25
MflI RGATCY 6 cut(s) 313, 354, 1117, 1540, 1900, 1936
MhlI GDGCHC 2 cut(s) 670, 2001
Mly113I GGCGCC 1 cut(s) 756
MlyI GAGTC 2 cut(s) 415, 443
MmeI TCCRAC 2 cut(s) 719, 1611
Mph1103I ATGCAT 1 cut(s) 1573
MroI TCCGGA 1 cut(s) 763
MseI TTAA 8 cut(s) 249, 521, 675, 1167, 1686, 1968, 1974, 2304
MslI CAYNNNNRTG 2 cut(s) 1026, 1882
MspA1I CMGCKG 1 cut(s) 851
MspCI CTTAAG 1 cut(s) 1973
MspI CCGG 2 cut(s) 764, 2079
MspR9I CCNGG 2 cut(s) 196, 1518
MssI GTTTAAAC 1 cut(s) 676
MunI CAATTG 1 cut(s) 25
Mva1269I GAATGC 2 cut(s) 1082, 1497
MvaI CCWGG 2 cut(s) 196, 1518
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 4 cut(s) 1228, 1465, 1834, 1855
NarI GGCGCC 1 cut(s) 756
NdeI CATATG 1 cut(s) 1347
NlaIV GGNNCC 4 cut(s) 315, 634, 757, 1542
NmuCI GTSAC 1 cut(s) 1354
NsiI ATGCAT 1 cut(s) 1573
NspI RCATGY 6 cut(s) 1058, 1145, 1335, 1575, 1887, 2047
PaeI GCATGC 2 cut(s) 1058, 1575
PaeR7I CTCGAG 2 cut(s) 1958, 2219
PciI ACATGT 1 cut(s) 1883
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 685
PctI GAATGC 2 cut(s) 1082, 1497
PfeI GAWTC 7 cut(s) 191, 536, 599, 969, 1278, 2082, 2224
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 4 cut(s) 591, 789, 853, 1224
PleI GAGTC 2 cut(s) 415, 443
PluTI GGCGCC 1 cut(s) 759
PmeI GTTTAAAC 1 cut(s) 676
PpsI GAGTC 2 cut(s) 415, 443
Ppu21I YACGTR 1 cut(s) 1247
PscI ACATGT 1 cut(s) 1883
Psp124BI GAGCTC 2 cut(s) 670, 2001
Psp6I CCWGG 2 cut(s) 194, 1516
PspGI CCWGG 2 cut(s) 194, 1516
PspN4I GGNNCC 4 cut(s) 315, 634, 757, 1542
PspPI GGNCC 3 cut(s) 165, 1647, 1770
PsrI GAACNNNNNNTAC 2 cut(s) 616, 648
PsuI RGATCY 6 cut(s) 313, 354, 1117, 1540, 1900, 1936
PvuII CAGCTG 1 cut(s) 851
RsaI GTAC 5 cut(s) 921, 1582, 1638, 1750, 1841
RsaNI GTAC 5 cut(s) 920, 1581, 1637, 1749, 1840
RseI CAYNNNNRTG 2 cut(s) 1026, 1882
SacI GAGCTC 2 cut(s) 670, 2001
SaqAI TTAA 8 cut(s) 249, 521, 675, 1167, 1686, 1968, 1974, 2304
SatI GCNGC 4 cut(s) 590, 788, 852, 1223
Sau96I GGNCC 3 cut(s) 165, 1647, 1770
SchI GAGTC 2 cut(s) 415, 443
ScrFI CCNGG 2 cut(s) 196, 1518
SduI GDGCHC 2 cut(s) 670, 2001
SfaNI GCATC 3 cut(s) 340, 564, 1446
SfoI GGCGCC 1 cut(s) 757
Sfr274I CTCGAG 2 cut(s) 1958, 2219
SinI GGWCC 1 cut(s) 1770
SlaI CTCGAG 2 cut(s) 1958, 2219
SmiMI CAYNNNNRTG 2 cut(s) 1026, 1882
SmlI CTYRAG 5 cut(s) 46, 1232, 1958, 1973, 2219
SmoI CTYRAG 5 cut(s) 46, 1232, 1958, 1973, 2219
SnaBI TACGTA 1 cut(s) 1247
SphI GCATGC 2 cut(s) 1058, 1575
SsiI CCGC 4 cut(s) 70, 1145, 1189, 2104
SspDI GGCGCC 1 cut(s) 755
SspI AATATT 3 cut(s) 745, 1184, 1621
SspMI CTAG 3 cut(s) 923, 1403, 1412
SstI GAGCTC 2 cut(s) 670, 2001
StyD4I CCNGG 2 cut(s) 194, 1516
StyI CCWWGG 3 cut(s) 531, 697, 1226
TaaI ACNGT 4 cut(s) 1256, 1463, 1712, 2031
TaiI ACGT 2 cut(s) 69, 1249
TatI WGTACW 2 cut(s) 1636, 1839
TfiI GAWTC 7 cut(s) 191, 536, 599, 969, 1278, 2082, 2224
Tru1I TTAA 8 cut(s) 249, 521, 675, 1167, 1686, 1968, 1974, 2304
Tru9I TTAA 8 cut(s) 249, 521, 675, 1167, 1686, 1968, 1974, 2304
TscAI CASTG 3 cut(s) 127, 1582, 1611
TseFI GTSAC 1 cut(s) 1354
TseI GCWGC 4 cut(s) 589, 787, 851, 1222
Tsp45I GTSAC 1 cut(s) 1354
TspDTI ATGAA 5 cut(s) 222, 700, 794, 1044, 1421
TspGWI ACGGA 3 cut(s) 71, 1661, 1923
TspRI CASTG 3 cut(s) 127, 1582, 1611
Vha464I CTTAAG 1 cut(s) 1973
VpaK11BI GGWCC 1 cut(s) 1770
XapI RAATTY 5 cut(s) 813, 1529, 1775, 2067, 2147
XceI RCATGY 6 cut(s) 1058, 1145, 1335, 1575, 1887, 2047
XcmI CCANNNNNNNNNTGG 2 cut(s) 202, 502
XhoI CTCGAG 2 cut(s) 1958, 2219
XmiI GTMKAC 1 cut(s) 1243
XspI CTAG 3 cut(s) 923, 1403, 1412
ZraI GACGTC 1 cut(s) 67
Zsp2I ATGCAT 1 cut(s) 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.