AT5G46260

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002912
Physical Location & Seq
Reverse (-)
18758699 .. 18763503
4805 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46260.1

Sequence Viewer

Length: 3618 bp
ATGGCTTCTTCATCTTCTTCTCGAAATTGGTTATATGATGTTTTCTTGAGCTTCAGAGGGGGAGACGTCCGTGTAACATTCCGTAGCCACTTTCTCAAGGAGTTCGATCGGAAACTGATCACTGCTTTCAGAGACAATGAGATCGAGAGAAGCCACTCTCTCTGGCCCGATCTTGAACAAGCCATCAAGGAATCGAGGATCGCAGTGGTCGTTTTCTCCAAAAACTACGCTTCATCGAGCTGGTGTCTTAACGAGTTGCTGGAGATTGTGAACTGCAATGATAAAATTATCATACCGGTTTTCTACGGTGTGGATCCTTCCCAAGTGAGGTATCAAATCGGTGAATTCGGGAAGATCTTTGAAAAGACATGCAAGAGACAAACAGAGGAAGTGAAAAACCAATGGAAGAAAGCGTTGACTCATGTAGCTAATATGCTTGGATTTGATTCTTCCAAATGGGATGACGAAGCAAAAATGATTGAAGAAATAGCCAATGATGTTTTGCGTAAACTGCTTTTAACTACATCGAAGGATTTTGAGGACTTTGTCGGCCTCGAGGATCATATTGCAAATATGAGTGCACTGTTGGATCTGGAATCTAAGGAAGTGAAGATGGTTGGTATATGGGGTTCCTCGGGGATTGGTAAGACTACCATTGCACGAGCTTTGTTTAACAACCTTTTTCGGCATTTCCAAGTTAGGAAATTCATAGACAGATCTTTTGCCTATAAGAGTAGAGAAATTCATAGTAGTGCCAATCCTGACGACCACAATATGAAGTTACATTTACAAGAGAGCTTCCTCTCTGAAATTCTAAGGATGCCGAACATAAAGATAGATCATCTAGGTGTACTAGGAGAGAGGCTACAACACCAGAAAGTTCTTATCATTATTGATGATGTGGATGATCAAGTGATCTTAGATTCATTGGTGGGTAAAACTCAATGGTTTGGAAATGGGAGCAGAATCATTGTGGTTACAAATAATAAACATTTTTTAACGGCTCATGGAATTGATCGTATGTATGAAGTTAGTCTCCCAACGGAAGAGCACGCTCTTGCAATGTTATGTCAATCTGCTTTCAAGAAAAAATCTCCACCTGAAGGTTTTGAGATGCTTGTAGTTCAAGTTGCAAGATATGCTGGTAGTCTTCCTCTGGTTCTTAAGGTTTTGGGTTCATATTTGAGTGGGAAGGATAAGGAGTACTGGATAGATATGCTGCCAAGGCTTCAAAATGGTTTAAACGATAAAATTGAGAGAATACTAAGAATCAGCTATGATGGGTTAGAGAGTGAAGATCAAGCGATATTTCGTCATATCGCATGTATTTTCAATCATATGGAAGTCACAACCATCAAGTCATTGCTCGCAAATAGTATATATGGTGCTAATGTTGGGCTGCAAAACCTGGTTGATAAGTCCATCATTCATGTCAGATGGGGTCATGTGGAGATGCATCCTTTGCTACAAGAAATGGGCAGAAAAATTGTTCGCACACAGTCCATTGGCAAGCCCCGAAAACGAGAATTTCTGGTGGATCCAAATGATATTTGTGATGTACTCAGTGAAGGCATTGATACTCAAAAGGTTTTAGGTATATCATTGGAGACAAGTAAGATTGATGAGTTGTGTGTACATGAGAGTGCCTTCAAACGGATGCGTAATCTCCGTTTCTTAAAAATTGGTACGGATATCTTTGGAGAAGAAAACAGATTGCATTTACCTGAAAGTTTCGACTATTTGCCTCCTACACTTAAACTATTGTGCTGGTCCGAATTTCCAATGAGGTGTATGCCTTCTAATTTTTGCCCTAAAAACCTTGTCACGCTCAAAATGACGAATAGTAAGCTACATAAGTTGTGGGAAGGAGCTGTGCCACTTACATGTCTAAAGGAAATGGATCTAGATGGATCAGTCAATCTGAAAGAAATTCCAGATCTTTCCATGGCTACTAATCTCGAGACACTTAATTTTGAGAATTGCAAGAGTTTGGTGGAGCTTCCTTCCTTTATACAAAATCTCAATAAGCTGTTGAAATTGAACATGGCATTCTGCAACAGTCTGGAAACTCTTCCAACTGGTTTTAACCTCAAATCTTTGAACCGCATCGATTTCACTAAATGCTCGAAGTTAAGGACTTTTCCGGACTTCTCAACCAACATCTCAGATCTCTATTTGACTGGAACAAACATTGAAGAACTCCCTTCTAATTTACATCTCGAGAATCTTATTGATCTTAGAATATCAAAAAAGGAGATTGATGGGAAACAATGGGAAGGAGTGATGAAGCCGCTTAAACCCTTACTGGCGATGTTGTCTCCCACTTTGACGAGCTTGCAACTCCAGAACATCCCAAATTTGGTGGAGCTTCCTTGCTCATTTCAGAATCTCATTCAACTGGAGGTTTTGGACATCACAAACTGTAGAAATCTGGAGACTCTGCCCACCGGAATCAACCTCCAATCTCTCGATAGCCTTAGTTTCAAGGGATGCTCACGGTTGAGGAGCTTTCCTGAAATCTCAACCAACATCTCAAGTCTCAATCTAGAGGAAACAGGGATTGAAGAAGTTCCTTGGTGGATCGATAAATTCTCTAACCTCGGTTTGCTAAGTATGGATCGTTGCAGCAGGCTAAAATGTGTATCCCTACACATTTCTAAACTGAAACGTCTTGGAAAAGTTGACTTTAAAGATTGTGGGGCGTTGACTATAGTTGATTTGTGTGGCTGTCCAATTGGGATGGAGATGGAAGCAAACAATATCGACACAGTTTCCAAAGTCAAACTTGATTTCAGGGACTGCTTCAACTTGGATCCAGAAACTGTACTTCACCAAGAATCAATTATTTTCAAGTACATGTTGTTTCCAGGGAAAGAAGAAATGCCATCATATTTCACTTACCGTACTACTGGAAGCTCCTCTCTGACCATTCCTCTACTTCACCTCCCTCTCTCCCAACCATTCTTCAGATTTAGGGTTGGCGCATTGGTAACTAATGTTAAGCATGGAAAAAATATAAAGGTGAAGTGTGAGTTCAAAGACAGATTTGGGAACAGCTTTCATGTTGGCTCGGATGATTTCTACGTTTATCTACTTTTCACGAAAAGTCAGAAGGGTAGTCAAATGTTAACTATATTGGACTGTTGTATCCCTCTAAACGAAGGTAATGCTTCTCTAGCTCAAGGGAACTACTACGATCATGTGGATATAAATATTCATATAAGTAGTGGAGGTTGGAGATCTACGTTTGAATTAAAAGAATGGGGTATACGACTCTTAGAGGAGGACTCTTCATCAGCAGAGAACGGACTTGGTAATCCAAACAGTACTCTTCCACATGTTTCTGAAGCCGAAGAAGGCAATATGGGGTATTATACACCTCTTCAAGAAGGACTTGTTAATGAGATTGAACACAGTGAAGAGTCTGGAGATATCAATGTAGGGACTAAGAGAAGCAAGAAGCGAATGCGGCCGAAGAAGACAATATGGGGTATTCACCGCTTCAAGAACTTACTGAGAAGCAGGAAGCGAATGCGTGTAAGCATTAAAAATGAAAGACCACTCAAGAAATCTAGGACTCGCCCTGCCTCAATACCTAGGACTGGGAGGAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1205

Amino Acids

137.47

Weight (kDa)

7.71

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 184 8.6e-55 TIR domain
TIR_2 PF13676 14 - 106 6.1e-10 TIR domain
AAA_16 PF13191 182 - 303 9.4e-06 AAA ATPase domain
NB-ARC PF00931 198 - 364 1.2e-13 NB-ARC domain
WHD_ROQ1 PF23282 431 - 501 2e-25 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 606 - 623 8.7e-07 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 69
AbsI CCTCGAGG 1 cut(s) 554
AccI GTMKAC 1 cut(s) 3268
AccIII TCCGGA 1 cut(s) 2143
AciI CCGC 4 cut(s) 2104, 2291, 3469, 3499
AcoI YGGCCR 1 cut(s) 3470
AcsI RAATTY 9 cut(s) 344, 704, 741, 810, 1526, 1775, 1929, 2356, 2588
AcuI CTGAAG 4 cut(s) 37, 1122, 2950, 3366
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 9 cut(s) 852, 1205, 1560, 1635, 1687, 2826, 2855, 2905, 3328
AfiI CCNNNNNNNGG 5 cut(s) 327, 1103, 1653, 2359, 3602
AflII CTTAAG 1 cut(s) 1163
AflIII ACRYGT 3 cut(s) 1883, 2856, 3337
AgeI ACCGGT 1 cut(s) 295
AjnI CCWGG 2 cut(s) 1407, 2866
Alw21I GWGCWC 2 cut(s) 583, 1053
Alw26I GTCTC 9 cut(s) 57, 126, 370, 1040, 1601, 1955, 2322, 2429, 2543
Alw44I GTGCAC 1 cut(s) 579
AlwNI CAGNNNCTG 2 cut(s) 2799, 2822
Ama87I CYCGRG 4 cut(s) 554, 634, 1958, 2219
Aor13HI TCCGGA 1 cut(s) 2143
AoxI GGCC 3 cut(s) 164, 550, 3470
ApaLI GTGCAC 1 cut(s) 579
ApeKI GCWGC 3 cut(s) 1219, 1399, 2625
ApoI RAATTY 9 cut(s) 344, 704, 741, 810, 1526, 1775, 1929, 2356, 2588
ArsI GACNNNNNNTTYG 2 cut(s) 704, 736
AsiGI ACCGGT 1 cut(s) 295
Asp700I GAANNNNTTC 2 cut(s) 2070, 2568
AspA2I CCTAGG 1 cut(s) 3596
AspLEI GCGC 1 cut(s) 2984
AspS9I GGNCC 2 cut(s) 165, 1770
AsuHPI GGTGA 5 cut(s) 353, 2822, 2933, 3034, 3488
AvaI CYCGRG 4 cut(s) 554, 634, 1958, 2219
AvaII GGWCC 1 cut(s) 1770
AvrII CCTAGG 1 cut(s) 3596
BaeGI GKGCMC 1 cut(s) 583
BaeI ACNNNNGTAYC 2 cut(s) 3130, 3163
BamHI GGATCC 3 cut(s) 313, 1537, 2812
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 2 cut(s) 1142, 3485
Bbv12I GWGCWC 2 cut(s) 583, 1053
BbvI GCAGC 3 cut(s) 1206, 1386, 2637
BceAI ACGGC 1 cut(s) 1017
BcgI CGANNNNNNTGC 2 cut(s) 3149, 3183
BciT130I CCWGG 2 cut(s) 1409, 2868
BciVI GTATCC 2 cut(s) 2653, 3158
BclI TGATCA 2 cut(s) 117, 907
BcoDI GTCTC 9 cut(s) 57, 126, 370, 1040, 1601, 1955, 2322, 2429, 2543
BfaI CTAG 7 cut(s) 845, 854, 1904, 2546, 3176, 3573, 3597
BfmI CTRYAG 2 cut(s) 2422, 2709
BfrI CTTAAG 1 cut(s) 1163
BfuI GTATCC 2 cut(s) 2653, 3158
BglII AGATCT 5 cut(s) 354, 716, 1936, 2167, 3239
BisI GCNGC 5 cut(s) 1220, 1400, 2291, 2626, 3470
BlnI CCTAGG 1 cut(s) 3596
BlsI GCNGC 5 cut(s) 1221, 1401, 2292, 2627, 3471
BmcAI AGTACT 2 cut(s) 1205, 3328
Bme1390I CCNGG 2 cut(s) 1409, 2868
Bme18I GGWCC 1 cut(s) 1770
BmeT110I CYCGRG 4 cut(s) 554, 634, 1958, 2219
BmgT120I GGNCC 2 cut(s) 165, 1770
BmiI GGNNCC 4 cut(s) 315, 631, 1539, 2814
BmrFI CCNGG 2 cut(s) 1409, 2868
BmrI ACTGGG 1 cut(s) 3612
BmsI GCATC 7 cut(s) 810, 1104, 1443, 1465, 1647, 2115, 2480
BmuI ACTGGG 1 cut(s) 3612
BpiI GAAGAC 2 cut(s) 1142, 3485
BplI GAGNNNNNCTC 2 cut(s) 3272, 3304
BpmI CTGGAG 5 cut(s) 281, 2327, 2420, 2453, 3447
BpuEI CTTGAG 5 cut(s) 67, 80, 2518, 3165, 3548
Bsa29I ATCGAT 2 cut(s) 2109, 2583
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 7 cut(s) 633, 1223, 1944, 2573, 2599, 2867, 3596
BsaWI WCCGGW 3 cut(s) 295, 2143, 2447
BsaXI ACNNNNNCTCC 2 cut(s) 2928, 2958
Bsc4I CCNNNNNNNGG 5 cut(s) 327, 1103, 1653, 2359, 3602
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 7 cut(s) 1211, 2083, 2185, 2310, 2403, 2914, 3607
Bse3DI GCAATG 4 cut(s) 283, 654, 1068, 1361
BseAI TCCGGA 1 cut(s) 2143
BseBI CCWGG 2 cut(s) 1409, 2868
BseCI ATCGAT 2 cut(s) 2109, 2583
BseDI CCNNGG 7 cut(s) 633, 1223, 1944, 2573, 2599, 2867, 3596
BseGI GGATG 9 cut(s) 466, 825, 910, 1456, 1662, 2349, 2495, 2745, 3079
BseLI CCNNNNNNNGG 5 cut(s) 327, 1103, 1653, 2359, 3602
BseMI GCAATG 4 cut(s) 283, 654, 1068, 1361
BseMII CTCAG 3 cut(s) 1576, 2178, 3506
BseNI ACTGG 7 cut(s) 1211, 2083, 2185, 2310, 2403, 2914, 3607
BseRI GAGGAG 3 cut(s) 2518, 2908, 3296
BseSI GKGCMC 1 cut(s) 583
BseX3I CGGCCG 1 cut(s) 3470
BseXI GCAGC 3 cut(s) 1206, 1386, 2637
Bsh1285I CGRYCG 2 cut(s) 109, 3473
BshFI GGCC 3 cut(s) 166, 552, 3472
BshTI ACCGGT 1 cut(s) 295
BshVI ATCGAT 2 cut(s) 2109, 2583
BsiEI CGRYCG 2 cut(s) 109, 3473
BsiHKAI GWGCWC 2 cut(s) 583, 1053
BsiHKCI CYCGRG 4 cut(s) 554, 634, 1958, 2219
BsiSI CCGG 3 cut(s) 296, 2144, 2448
BslFI GGGAC 2 cut(s) 2810, 3457
BslI CCNNNNNNNGG 5 cut(s) 327, 1103, 1653, 2359, 3602
BsmAI GTCTC 9 cut(s) 57, 126, 370, 1040, 1601, 1955, 2322, 2429, 2543
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 2 cut(s) 2810, 3457
BsmI GAATGC 3 cut(s) 2048, 3471, 3537
BsnI GGCC 3 cut(s) 166, 552, 3472
BsoBI CYCGRG 4 cut(s) 554, 634, 1958, 2219
Bsp1286I GDGCHC 2 cut(s) 583, 1053
Bsp13I TCCGGA 1 cut(s) 2143
Bsp1407I TGTACA 1 cut(s) 1633
Bsp19I CCATGG 1 cut(s) 1944
BspACI CCGC 4 cut(s) 2104, 2291, 3469, 3499
BspANI GGCC 3 cut(s) 166, 552, 3472
BspCNI CTCAG 3 cut(s) 1575, 2177, 3507
BspDI ATCGAT 2 cut(s) 2109, 2583
BspEI TCCGGA 1 cut(s) 2143
BspLI GGNNCC 4 cut(s) 315, 631, 1539, 2814
BspQI GCTCTTC 1 cut(s) 1041
BspTI CTTAAG 1 cut(s) 1163
BsrDI GCAATG 4 cut(s) 283, 654, 1068, 1361
BsrFI RCCGGY 1 cut(s) 295
BsrGI TGTACA 1 cut(s) 1633
BsrI ACTGG 7 cut(s) 1211, 2083, 2185, 2310, 2403, 2914, 3607
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 7 cut(s) 633, 1223, 1944, 2573, 2599, 2867, 3596
BssNAI GTATAC 1 cut(s) 3269
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 4 cut(s) 1223, 1944, 2573, 3596
Bst1107I GTATAC 1 cut(s) 3269
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 2 cut(s) 1409, 2868
Bst6I CTCTTC 6 cut(s) 1041, 2076, 3295, 3336, 3387, 3414
BstACI GRCGYC 1 cut(s) 66
BstAFI CTTAAG 1 cut(s) 1163
BstAPI GCANNNNNTGC 2 cut(s) 1139, 1462
BstAUI TGTACA 1 cut(s) 1633
BstC8I GCNNGC 5 cut(s) 1053, 1368, 1511, 2336, 2630
BstDSI CCRYGG 1 cut(s) 1944
BstF5I GGATG 9 cut(s) 466, 825, 910, 1456, 1662, 2349, 2495, 2745, 3079
BstHHI GCGC 1 cut(s) 2984
BstMAI GTCTC 9 cut(s) 57, 126, 370, 1040, 1601, 1955, 2322, 2429, 2543
BstMCI CGRYCG 2 cut(s) 109, 3473
BstMWI GCNNNNNNNGC 6 cut(s) 511, 1139, 1225, 1462, 3176, 3469
BstNI CCWGG 2 cut(s) 1409, 2868
BstNSI RCATGY 5 cut(s) 372, 1326, 1887, 2860, 3341
BstSCI CCNGG 2 cut(s) 1407, 2866
BstSFI CTRYAG 2 cut(s) 2422, 2709
BstSLI GKGCMC 1 cut(s) 583
BstV1I GCAGC 3 cut(s) 1206, 1386, 2637
BstV2I GAAGAC 2 cut(s) 1142, 3485
BstZ17I GTATAC 1 cut(s) 3269
BstZI CGGCCG 1 cut(s) 3470
Bsu15I ATCGAT 2 cut(s) 2109, 2583
BsuI GTATCC 2 cut(s) 2653, 3158
BsuRI GGCC 3 cut(s) 166, 552, 3472
BsuTUI ATCGAT 2 cut(s) 2109, 2583
BtgI CCRYGG 1 cut(s) 1944
BtgZI GCGATG 1 cut(s) 2324
BtsCI GGATG 9 cut(s) 466, 825, 910, 1456, 1662, 2349, 2495, 2745, 3079
BtsI GCAGTG 2 cut(s) 120, 210
BtsIMutI CAGTG 5 cut(s) 120, 210, 581, 1570, 3421
Cac8I GCNNGC 5 cut(s) 1053, 1368, 1511, 2336, 2630
CaiI CAGNNNCTG 2 cut(s) 2799, 2822
CfoI GCGC 1 cut(s) 2984
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 2 cut(s) 165, 1770
ClaI ATCGAT 2 cut(s) 2109, 2583
CsiI ACCWGGT 1 cut(s) 1407
Csp6I GTAC 9 cut(s) 851, 1204, 1559, 1634, 1686, 2825, 2854, 2904, 3327
CspAI ACCGGT 1 cut(s) 295
CspCI CAANNNNNGTGG 2 cut(s) 2343, 2378
CviQI GTAC 9 cut(s) 851, 1204, 1559, 1634, 1686, 2825, 2854, 2904, 3327
DraI TTTAAA 2 cut(s) 1242, 2689
EaeI YGGCCR 1 cut(s) 3470
EagI CGGCCG 1 cut(s) 3470
Eam1104I CTCTTC 6 cut(s) 1041, 2076, 3295, 3336, 3387, 3414
EarI CTCTTC 6 cut(s) 1041, 2076, 3295, 3336, 3387, 3414
EclXI CGGCCG 1 cut(s) 3470
Eco130I CCWWGG 4 cut(s) 1223, 1944, 2573, 3596
Eco32I GATATC 2 cut(s) 1693, 3433
Eco47I GGWCC 1 cut(s) 1770
Eco52I CGGCCG 1 cut(s) 3470
Eco57I CTGAAG 4 cut(s) 37, 1122, 2950, 3366
Eco88I CYCGRG 4 cut(s) 554, 634, 1958, 2219
EcoRI GAATTC 1 cut(s) 344
EcoRII CCWGG 2 cut(s) 1407, 2866
EcoRV GATATC 2 cut(s) 1693, 3433
EcoT14I CCWWGG 4 cut(s) 1223, 1944, 2573, 3596
EcoT22I ATGCAT 1 cut(s) 1458
ErhI CCWWGG 4 cut(s) 1223, 1944, 2573, 3596
Esp3I CGTCTC 1 cut(s) 57
FalI AAGNNNNNCTT 4 cut(s) 2769, 2801, 3378, 3410
FaqI GGGAC 2 cut(s) 2810, 3457
FauNDI CATATG 1 cut(s) 1338
FbaI TGATCA 2 cut(s) 117, 907
FblI GTMKAC 1 cut(s) 3268
Fnu4HI GCNGC 5 cut(s) 1220, 1400, 2291, 2626, 3470
FokI GGATG 9 cut(s) 473, 832, 917, 1443, 1669, 2336, 2502, 2752, 3086
Fsp4HI GCNGC 5 cut(s) 1220, 1400, 2291, 2626, 3470
FspBI CTAG 7 cut(s) 845, 854, 1904, 2546, 3176, 3573, 3597
GlaI GCGC 1 cut(s) 2983
GluI GCNGC 5 cut(s) 1220, 1400, 2291, 2626, 3470
GsuI CTGGAG 5 cut(s) 281, 2327, 2420, 2453, 3447
HaeIII GGCC 3 cut(s) 166, 552, 3472
HapII CCGG 3 cut(s) 296, 2144, 2448
HhaI GCGC 1 cut(s) 2984
Hin1I GRCGYC 1 cut(s) 66
Hin6I GCGC 1 cut(s) 2982
HinP1I GCGC 1 cut(s) 2982
HincII GTYRAC 4 cut(s) 417, 2683, 2706, 3129
HindII GTYRAC 4 cut(s) 417, 2683, 2706, 3129
HpaI GTTAAC 1 cut(s) 3129
HpaII CCGG 3 cut(s) 296, 2144, 2448
HphI GGTGA 5 cut(s) 353, 2822, 2933, 3034, 3488
HpyCH4IV ACGT 4 cut(s) 66, 2668, 3084, 3245
HpyF10VI GCNNNNNNNGC 6 cut(s) 511, 1139, 1225, 1462, 3176, 3469
HpySE526I ACGT 4 cut(s) 66, 2668, 3084, 3245
Hsp92I GRCGYC 1 cut(s) 66
HspAI GCGC 1 cut(s) 2982
Kpn2I TCCGGA 1 cut(s) 2143
Ksp22I TGATCA 2 cut(s) 117, 907
KspAI GTTAAC 1 cut(s) 3129
LguI GCTCTTC 1 cut(s) 1041
LmnI GCTCC 6 cut(s) 960, 1868, 1996, 2365, 2505, 2921
Lsp1109I GCAGC 3 cut(s) 1206, 1386, 2637
LweI GCATC 7 cut(s) 810, 1104, 1443, 1465, 1647, 2115, 2480
MabI ACCWGGT 1 cut(s) 1407
MaeI CTAG 7 cut(s) 845, 854, 1904, 2546, 3176, 3573, 3597
MaeII ACGT 4 cut(s) 66, 2668, 3084, 3245
MaeIII GTNAC 6 cut(s) 73, 780, 976, 1345, 1822, 2989
MfeI CAATTG 1 cut(s) 2733
MhlI GDGCHC 2 cut(s) 583, 1053
MlyI GAGTC 6 cut(s) 412, 2431, 3267, 3281, 3431, 3571
MmeI TCCRAC 3 cut(s) 567, 2099, 3215
Mph1103I ATGCAT 1 cut(s) 1458
MroI TCCGGA 1 cut(s) 2143
MroXI GAANNNNTTC 2 cut(s) 2070, 2568
MslI CAYNNNNRTG 4 cut(s) 750, 846, 1641, 1882
MspCI CTTAAG 1 cut(s) 1163
MspI CCGG 3 cut(s) 296, 2144, 2448
MspR9I CCNGG 2 cut(s) 1409, 2868
MssI GTTTAAAC 1 cut(s) 1242
MunI CAATTG 1 cut(s) 2733
Mva1269I GAATGC 3 cut(s) 2048, 3471, 3537
MvaI CCWGG 2 cut(s) 1409, 2868
MwoI GCNNNNNNNGC 6 cut(s) 511, 1139, 1225, 1462, 3176, 3469
NcoI CCATGG 1 cut(s) 1944
NdeI CATATG 1 cut(s) 1338
NlaIV GGNNCC 4 cut(s) 315, 631, 1539, 2814
NmuCI GTSAC 2 cut(s) 1345, 1822
NsiI ATGCAT 1 cut(s) 1458
NspI RCATGY 5 cut(s) 372, 1326, 1887, 2860, 3341
PaeR7I CTCGAG 3 cut(s) 554, 1958, 2219
PciI ACATGT 3 cut(s) 1883, 2856, 3337
PciSI GCTCTTC 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 3 cut(s) 2048, 3471, 3537
PdmI GAANNNNTTC 2 cut(s) 2070, 2568
PflFI GACNNNGTC 1 cut(s) 545
PinAI ACCGGT 1 cut(s) 295
PkrI GCNGC 5 cut(s) 1221, 1401, 2292, 2627, 3471
Ple19I CGATCG 1 cut(s) 109
PleI GAGTC 6 cut(s) 412, 2431, 3267, 3281, 3430, 3571
PmeI GTTTAAAC 1 cut(s) 1242
PpsI GAGTC 6 cut(s) 412, 2431, 3267, 3281, 3430, 3571
PscI ACATGT 3 cut(s) 1883, 2856, 3337
Psp6I CCWGG 2 cut(s) 1407, 2866
PspGI CCWGG 2 cut(s) 1407, 2866
PspN4I GGNNCC 4 cut(s) 315, 631, 1539, 2814
PspPI GGNCC 2 cut(s) 165, 1770
PspXI VCTCGAGB 1 cut(s) 554
PsrI GAACNNNNNNTAC 2 cut(s) 613, 645
PstNI CAGNNNCTG 2 cut(s) 2799, 2822
PsyI GACNNNGTC 1 cut(s) 545
PvuI CGATCG 1 cut(s) 109
RsaI GTAC 9 cut(s) 852, 1205, 1560, 1635, 1687, 2826, 2855, 2905, 3328
RsaNI GTAC 9 cut(s) 851, 1204, 1559, 1634, 1686, 2825, 2854, 2904, 3327
RseI CAYNNNNRTG 4 cut(s) 750, 846, 1641, 1882
SapI GCTCTTC 1 cut(s) 1041
SatI GCNGC 5 cut(s) 1220, 1400, 2291, 2626, 3470
Sau96I GGNCC 2 cut(s) 165, 1770
ScaI AGTACT 2 cut(s) 1205, 3328
SchI GAGTC 6 cut(s) 412, 2431, 3267, 3281, 3431, 3571
ScrFI CCNGG 2 cut(s) 1409, 2868
SduI GDGCHC 2 cut(s) 583, 1053
SexAI ACCWGGT 1 cut(s) 1407
SfaNI GCATC 7 cut(s) 810, 1104, 1443, 1465, 1647, 2115, 2480
SfcI CTRYAG 2 cut(s) 2422, 2709
Sfr274I CTCGAG 3 cut(s) 554, 1958, 2219
SinI GGWCC 1 cut(s) 1770
SlaI CTCGAG 3 cut(s) 554, 1958, 2219
SmiMI CAYNNNNRTG 4 cut(s) 750, 846, 1641, 1882
SmlI CTYRAG 9 cut(s) 46, 95, 554, 1163, 1958, 2219, 2533, 3180, 3563
SmoI CTYRAG 9 cut(s) 46, 95, 554, 1163, 1958, 2219, 2533, 3180, 3563
SsiI CCGC 4 cut(s) 2104, 2291, 3469, 3499
SspI AATATT 1 cut(s) 3214
SspMI CTAG 7 cut(s) 845, 854, 1904, 2546, 3176, 3573, 3597
StyD4I CCNGG 2 cut(s) 1407, 2866
StyI CCWWGG 4 cut(s) 1223, 1944, 2573, 3596
TaiI ACGT 4 cut(s) 69, 2671, 3087, 3248
TatI WGTACW 7 cut(s) 850, 1203, 1558, 1633, 2824, 2853, 3326
TauI GCSGC 2 cut(s) 2293, 3472
TscAI CASTG 5 cut(s) 127, 210, 588, 1570, 3421
TseFI GTSAC 2 cut(s) 1345, 1822
TseI GCWGC 3 cut(s) 1219, 1399, 2625
Tsp45I GTSAC 2 cut(s) 1345, 1822
TspGWI ACGGA 7 cut(s) 59, 71, 1058, 1658, 1669, 1703, 3321
TspRI CASTG 5 cut(s) 127, 210, 588, 1570, 3421
Tth111I GACNNNGTC 1 cut(s) 545
Vha464I CTTAAG 1 cut(s) 1163
VneI GTGCAC 1 cut(s) 579
VpaK11BI GGWCC 1 cut(s) 1770
XapI RAATTY 9 cut(s) 344, 704, 741, 810, 1526, 1775, 1929, 2356, 2588
XbaI TCTAGA 2 cut(s) 1903, 2545
XceI RCATGY 5 cut(s) 372, 1326, 1887, 2860, 3341
XhoI CTCGAG 3 cut(s) 554, 1958, 2219
XmaJI CCTAGG 1 cut(s) 3596
XmiI GTMKAC 1 cut(s) 3268
XmnI GAANNNNTTC 2 cut(s) 2070, 2568
XspI CTAG 7 cut(s) 845, 854, 1904, 2546, 3176, 3573, 3597
ZraI GACGTC 1 cut(s) 67
ZrmI AGTACT 2 cut(s) 1205, 3328
Zsp2I ATGCAT 1 cut(s) 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.