AT5G46510

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002912
Physical Location & Seq
Forward (+)
18860234 .. 18867015
6782 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46510.1

Sequence Viewer

Length: 4062 bp
ATGGCTTCTTCTCGGAATTGGGTTTATGATGTCTTCCCGAGCTTCAGCGGAACAGATGTCCGTCGGAATTTCCTCAGCCACTTGCTCAAGGGGCTGCATAAATCCGTCAATTCTTTCAGAGACCAAAACATGGAGAGAAGCCAATCACTCGATCCCATGCTTAAACAGGCGATTAGGGATTCTAGAATTGCTCTGGTCGTGTTCTCAAAGAACTACGCCTCTTCAAGCTGGTGCCTTAATGAGCTGTTGGAGATTGTGAAATGTAAGGAAGAGTTTGGTCAAATGGTGATTCCTATTTTCTACTGTTTGGATCCTTCCCATGTGAGGCATCAAGACGGTGACTTTGGAAAGAACTTTGAAGAGACTTGCGGGAGAAATACAGAGGAAGAGAAAATTCAATGGGAGAAAGCGTTGACTGATGTCGCAAATCTCGCTGGGTTTGATTCTGTGACCTGGGATGACGAAGCAAAAATGATTGAAGAAATAGCCAATGATGTTTCGGGTAAACTGCTTTCAACTTCATCCACGGATTCTGCTGAGAACTCTATTGGCATTGAAGATCATATTGCAAATATGAGTGTCTTGTTGCAATTGGAAGCTGAGGAAGTGAGAATGGTTGGTATATGGGGTTCCTCGGGGATTGGTAAGACTACCATTGCAAGAGCTCTGTTTAACCAACTTTCTCGGCATTTCCCAGTTAGCAAATTCATAGACAGAGCTTTTGTATATAAGAGTAGAGAAACTTACAAGGGCGCCAATCCGGACGACCCGAACATGAAGTTGCACTTGCAAGGATGTTTCCTATCGGAAATTTTGGGGAAAAAAGACATAAAGATTGATCATTTAGGTGCACTGGGAGAGAGGCTAAAGCACCAGAAAACTCTCATCATCATTGATGATTTGGATGATCTAGTGGTACTAGATTCATTGGTGGGTAAAACTAATTGGTTTGGATGTGGGAGCAGGATCATAGTGATTACCAATAATAAGCAATTCTTAAGGGCCCATGGAATTGATCATATTTACGAAGTTAGTCTCCCTTCTAAAGAGCGCGCTCAAGAGATGTTTTGTCAGTCTGCTTTCGGGGAAAACTCTCCACCTGAAGGTTTTGAGGAGCTCGTAGTTGAAATTGCATGGCTTGCTGGTAGTCTTCCTTTGGGTCTTACTGTTTTTGGTTCAGCTCTACGAGGAAGAAAAAAGGAGTACTGGGTGAAGATGTTGCCAAGGCTTCAAAATGATCTAGATGGCAATATTGAGGAGACATTGAAAGTCAGCTATGATGCGATAGGCAACGTAAAAGATCAAGCGTTATTCCGTCTTATCGCATGTCTTTTCAATCACGTCAAAGTCAGAGACATTGAATTGTTGCTCGCAGATAGTGGCTTAGATGTAAATATTGCACTGGAAAACCTAGTTGATAAGTCCCTTATTCATGTCAGAAATGATCATGTGGAGATGCACCGTTTGCTACAAGAAACGGGTAGAAATATTGTACGCTCACAGTCCACTGACAACCCAGGAGAACGAGAATTTCTGGTGGATTCAAACGATAGTCGTACTGTACTCAGTGAAGGCATTGGTACTCGAAAGGTGCTAGGAATATCATTGGATACAAGTAAGGTTAGTGAGTTTTGTGTACATGAGAATGCCTTCAAAGGGATGGGTAATCTCCTTTTCTTGGATATTTCCTCGAAGACTTTTATAGAAGAAGAAGTCAAAGTGCATTTACCGGAAAAAATAAACTATTATTCCGTTCAACCCAAACAATTGATTTGGGATAGATTTCCACTGAAATGTATGCCTTATACTTTTCTTCGAAACCTCGTCAAGCTCGAAATGCATGACAGCAAGCTAGAGAAGCTGTGGGAAGGAGCTATGTCATTTACATGTCTAAAGGAATTGGATATGTGGGCATCTAAATACCTGAAAGAAATCCCAGATCTTTCAAAGGCCACTAACATTGAGAAACTTGACTTTGGACATTGCTGGAGTTTGGTGGAGCTTCCTTCCTCTATACGAAATCTCAATAAACTGTTGGAATTGAATATGGAATACTGCGGTGAATTGGAGACTCTTCCTACGGGTTTCAACCTCAAATCCCTGGACTACCTCAATTTCAATGAATGCTGGAAGTTGAGGACTTTTCCTGAATTCGCAACCAACATCTCAAATCTCATTCTGGCAGAAACATCTATTGAAGAATACCCTTCCAATTTGTATTTCAAGAATGTCCGTGAGCTAAGCATGGGGAAAGCGGATAGTGATGAGAATAAATGCCAAGGAGTGAAGCCCTTCATGCCGATGCTGTCTCCCACTTTGACCCTCTTGGAGCTCTGGAATATCCCAAATTTAGTAGAGCTTTCGTCCTCATTTCAGAATCTCAATAACCTTGAGAGGTTGGACATTTGTTACTGCAGAAATCTGGAGTCTCTACCCACCGGAATCAACCTCGAATCTCTCGTTTCCCTTAATCTCTTTGGATGCTCACGGTTGAAGCGCTTTCCTGATATCTCAACCAACATCAAATATCTCGATCTAGACCAAACTGGGATTGAAGAGGTTCCTTGGCAGATCGAGAACTTCTTTAACCTCACTAAGCTAACTATGAAAGGTTGCCGCGAGCTAAAATGTGTATCCCTAAACATTTTTAAACTCAAACATCTTGGGGAAGTTAGCTTTTCCAATTGCGGGGCATTGACCAGAGTTGATTTGAGTTGCTATCCAAGTGGGGTGGAGATGATGAAGGCAGACAATGCTGACATAGTTTCTGAGGAGACCACCTCTTCTCTTCCTGATAGTTGCGTCCTCAATGTCAATTTCATGGATTGCGTCAATCTGGATCGAGAACCTGTCCTTCACCAACAGTCGATTATTTTCAACTCCATGATATTGCCAGGTGAAGAAGTGCCATCATATTTCACTTACCGAACATCTGACTCTCAACCATTTGGAACGTCTTCCTCTCTGCCCATCCCTCTACTTCCCACACAACTCTCTCAACCATTCTTCAGATTTAGGGTTTGTGCAGTGGTAAGTGCTTCCAATGGAGTATATATCGGGGTATATAGTCGGTTCAAAGGCAGAATTGGGAACAAATTTGATTCCTTTGGTGAGCTACATAAATTCATGGAAATTGAGAAGGGTATTCATCTGTGTATATTTGACTGTCGTATCCGTCTATACAAAGATAATGTTCCTCTATCTCAACTGAACTATGATCATGTGGATATAAATATTCATATAACTAGTGGAGATTGGAGATCTACGGTCGTTTTAAAAGAATGGGGTATACGACTCTTAGAGGACTGTTCATCAGCGGAGAACCGACTTGGTAATCCAAACAGTACTCTTCCACATGTTTGTGTAGAGGATGAAGAAAATTTGGTTAATGGGACTGAACAAGGTGATAGAGGTGATGTAGTGACGGAGACAAGCAGTAAACAGAATGAAGACGTTATCCTTCTGCAACGATCAGCTGATTCCATGTTCTCTAGGATGTCAGGAAAAGAAATGATTTCATATTTCACTCATCGCACTACTGGAACCTCTCTAACCAACATACCTCTACCTCACACCTCTCCCTCGCAACCGTTCTTCAGATTTAAAGCTTTCGCAGTGGTTGGTTGCAAATCTTCTGGTCAGTTTTCATATTTTGTCACCCATATTTCTTGTCGATTCAAAGATGGATTTGAGAATCAACCACGTAGGCTTTATAAAGCTGAGAATGGTAACCACCTTGACATATATGAGTGCTCTTTCCCTCTATACCAAGGTAATGCTTCTCCAGCTGAAATGAACTATGATCGCGTGGATATACAGCTTCATTTAAGGTATAGAAGTGACTCTACGGTCAGATTAATAGTATGGGGTATACGCCTCATCGAGGACTGTTCATCATCCGAGAACCAACTTGACAGTGAGACTGATCAGTTTGAAGAGTGTGGAAATAGTGATATAGGCCATGAGATTGATCAGAGTGAAGAATGTGAAGACATGGACGATACTGGTGGTATATGCATTGGGATTGATCAGAGTGAAGAGCGAGGAGACAGTGATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1353

Amino Acids

153.54

Weight (kDa)

5.09

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 9 - 180 2.9e-56 TIR domain
TIR_2 PF13676 11 - 102 1.8e-09 TIR domain
NB-ARC PF00931 198 - 363 4.6e-09 NB-ARC domain
WHD_ROQ1 PF23282 431 - 502 3e-39 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 607 - 624 3e-07 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3714
AasI GACNNNNNNGTC 1 cut(s) 3848
AccB1I GGYRCC 2 cut(s) 231, 752
AccB7I CCANNNNNTGG 1 cut(s) 130
AccI GTMKAC 2 cut(s) 3289, 3871
AccII CGCG 3 cut(s) 1053, 2619, 3807
AccIII TCCGGA 1 cut(s) 760
AciI CCGC 7 cut(s) 48, 369, 2058, 2255, 2617, 2688, 3317
AclWI GGATC 5 cut(s) 146, 305, 318, 974, 2847
AcuI CTGAAG 4 cut(s) 28, 1122, 2992, 3580
AcyI GRCGYC 1 cut(s) 753
AfaI GTAC 8 cut(s) 918, 1205, 1494, 1558, 1563, 1582, 1638, 3346
AfeI AGCGCT 1 cut(s) 2498
AfiI CCNNNNNNNGG 7 cut(s) 130, 324, 1103, 1656, 1678, 2688, 3883
AflII CTTAAG 1 cut(s) 997
AflIII ACRYGT 2 cut(s) 1886, 3355
AhlI ACTAGT 1 cut(s) 3245
AjiI CACGTC 1 cut(s) 1342
AjnI CCWGG 4 cut(s) 452, 1516, 2100, 2893
AjuI GAANNNNNNNTTGG 2 cut(s) 2204, 2236
Alw21I GWGCWC 5 cut(s) 667, 853, 1119, 2334, 3755
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 5 cut(s) 146, 305, 318, 974, 2847
Ama87I CYCGRG 2 cut(s) 37, 634
Aor13HI TCCGGA 1 cut(s) 760
Aor51HI AGCGCT 1 cut(s) 2498
AoxI GGCC 3 cut(s) 1002, 1950, 3958
ApaI GGGCCC 1 cut(s) 1006
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 1 cut(s) 94
ArsI GACNNNNNNTTYG 4 cut(s) 326, 358, 704, 736
AseI ATTAAT 1 cut(s) 3857
Asp700I GAANNNNTTC 4 cut(s) 1649, 2291, 2559, 2956
AspLEI GCGC 4 cut(s) 755, 1053, 1055, 2499
AspS9I GGNCC 2 cut(s) 1002, 1003
AsuII TTCGAA 1 cut(s) 1816
AvaI CYCGRG 2 cut(s) 37, 634
BaeGI GKGCMC 2 cut(s) 853, 1006
BaeI ACNNNNGTAYC 2 cut(s) 3154, 3187
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 2 cut(s) 231, 752
BanII GRGCYC 4 cut(s) 667, 1006, 1119, 2334
BarI GAAGNNNNNNTAC 2 cut(s) 3829, 3861
BbsI GAAGAC 6 cut(s) 25, 1142, 1700, 2949, 3456, 3996
Bbv12I GWGCWC 5 cut(s) 667, 853, 1119, 2334, 3755
BbvCI CCTCAGC 2 cut(s) 74, 600
BbvI GCAGC 1 cut(s) 81
BccI CCATC 5 cut(s) 1238, 1654, 2917, 2978, 3677
BciT130I CCWGG 4 cut(s) 454, 1518, 2102, 2895
BciVI GTATCC 3 cut(s) 1603, 2644, 3182
BclI TGATCA 7 cut(s) 838, 1015, 1444, 3217, 3925, 3970, 4027
BcuI ACTAGT 1 cut(s) 3245
BfmI CTRYAG 1 cut(s) 2413
BfoI RGCGCY 2 cut(s) 756, 2500
BfrI CTTAAG 1 cut(s) 997
BfuI GTATCC 3 cut(s) 1603, 2644, 3182
BglII AGATCT 2 cut(s) 1939, 3260
BisI GCNGC 2 cut(s) 95, 2617
BlpI GCTNAGC 1 cut(s) 2240
BlsI GCNGC 2 cut(s) 96, 2618
BmcAI AGTACT 2 cut(s) 1205, 3346
Bme1390I CCNGG 4 cut(s) 454, 1518, 2102, 2895
BmeT110I CYCGRG 2 cut(s) 37, 634
BmgBI CACGTC 1 cut(s) 1342
BmgT120I GGNCC 2 cut(s) 1002, 1003
BmiI GGNNCC 7 cut(s) 233, 312, 631, 754, 1004, 2562, 3544
BmrFI CCNGG 4 cut(s) 454, 1518, 2102, 2895
BmrI ACTGGG 4 cut(s) 689, 863, 1216, 2556
BmsI GCATC 6 cut(s) 337, 1270, 1446, 1922, 2292, 2471
BmuI ACTGGG 4 cut(s) 689, 863, 1216, 2556
BoxI GACNNNNGTC 1 cut(s) 419
BpiI GAAGAC 6 cut(s) 25, 1142, 1700, 2949, 3456, 3996
BplI GAGNNNNNCTC 2 cut(s) 2765, 2797
BpmI CTGGAG 3 cut(s) 2008, 2444, 3768
Bpu10I CCTNAGC 2 cut(s) 74, 600
Bpu1102I GCTNAGC 1 cut(s) 2240
Bpu14I TTCGAA 1 cut(s) 1816
BpuEI CTTGAG 3 cut(s) 71, 1041, 2411
BsaAI YACGTR 1 cut(s) 3704
BsaHI GRCGYC 1 cut(s) 753
BsaI GGTCTC 2 cut(s) 114, 2768
BsaWI WCCGGW 3 cut(s) 760, 1729, 2438
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 7 cut(s) 130, 324, 1103, 1656, 1678, 2688, 3883
Bse1I ACTGG 7 cut(s) 695, 858, 1211, 1407, 2551, 3544, 4009
Bse3DI GCAATG 2 cut(s) 654, 1981
BseAI TCCGGA 1 cut(s) 760
BseBI CCWGG 4 cut(s) 454, 1518, 2102, 2895
BseLI CCNNNNNNNGG 7 cut(s) 130, 324, 1103, 1656, 1678, 2688, 3883
BseMI GCAATG 2 cut(s) 654, 1981
BseMII CTCAG 6 cut(s) 88, 528, 591, 1579, 2760, 3711
BseNI ACTGG 7 cut(s) 695, 858, 1211, 1407, 2551, 3544, 4009
BsePI GCGCGC 1 cut(s) 1051
BseRI GAGGAG 4 cut(s) 1127, 1271, 2786, 4059
BseSI GKGCMC 2 cut(s) 853, 1006
BseXI GCAGC 1 cut(s) 81
BseYI CCCAGC 1 cut(s) 434
BsgI GTGCAG 1 cut(s) 3045
Bsh1236I CGCG 3 cut(s) 1053, 2619, 3807
Bsh1285I CGRYCG 1 cut(s) 3270
BshFI GGCC 3 cut(s) 1004, 1952, 3960
BshNI GGYRCC 2 cut(s) 231, 752
BsiEI CGRYCG 1 cut(s) 3270
BsiHKAI GWGCWC 5 cut(s) 667, 853, 1119, 2334, 3755
BsiHKCI CYCGRG 2 cut(s) 37, 634
BsiSI CCGG 3 cut(s) 761, 1730, 2439
BslFI GGGAC 2 cut(s) 1408, 3406
BslI CCNNNNNNNGG 7 cut(s) 130, 324, 1103, 1656, 1678, 2688, 3883
BsmFI GGGAC 2 cut(s) 1408, 3406
BsmI GAATGC 2 cut(s) 1651, 2129
BsnI GGCC 3 cut(s) 1004, 1952, 3960
Bso31I GGTCTC 2 cut(s) 114, 2768
BsoBI CYCGRG 2 cut(s) 37, 634
Bsp119I TTCGAA 1 cut(s) 1816
Bsp120I GGGCCC 1 cut(s) 1002
Bsp1286I GDGCHC 6 cut(s) 667, 853, 1006, 1119, 2334, 3755
Bsp13I TCCGGA 1 cut(s) 760
Bsp1407I TGTACA 1 cut(s) 1636
Bsp1720I GCTNAGC 1 cut(s) 2240
Bsp19I CCATGG 1 cut(s) 1006
BspACI CCGC 7 cut(s) 48, 369, 2058, 2255, 2617, 2688, 3317
BspANI GGCC 3 cut(s) 1004, 1952, 3960
BspCNI CTCAG 6 cut(s) 87, 529, 592, 1578, 2761, 3712
BspEI TCCGGA 1 cut(s) 760
BspFNI CGCG 3 cut(s) 1053, 2619, 3807
BspLI GGNNCC 7 cut(s) 233, 312, 631, 754, 1004, 2562, 3544
BspMAI CTGCAG 1 cut(s) 2417
BspPI GGATC 5 cut(s) 146, 305, 318, 974, 2847
BspQI GCTCTTC 1 cut(s) 4032
BspT104I TTCGAA 1 cut(s) 1816
BspT107I GGYRCC 2 cut(s) 231, 752
BspTI CTTAAG 1 cut(s) 997
BspTNI GGTCTC 2 cut(s) 114, 2768
BsrDI GCAATG 2 cut(s) 654, 1981
BsrGI TGTACA 1 cut(s) 1636
BsrI ACTGG 7 cut(s) 695, 858, 1211, 1407, 2551, 3544, 4009
BssHII GCGCGC 1 cut(s) 1051
BssNAI GTATAC 2 cut(s) 3290, 3872
BssNI GRCGYC 1 cut(s) 753
BssT1I CCWWGG 5 cut(s) 1006, 1223, 2278, 2564, 3769
Bst1107I GTATAC 2 cut(s) 3290, 3872
Bst2UI CCWGG 4 cut(s) 454, 1518, 2102, 2895
BstACI GRCGYC 1 cut(s) 753
BstAFI CTTAAG 1 cut(s) 997
BstAPI GCANNNNNTGC 3 cut(s) 1139, 1465, 2753
BstAUI TGTACA 1 cut(s) 1636
BstBAI YACGTR 1 cut(s) 3704
BstBI TTCGAA 1 cut(s) 1816
BstC8I GCNNGC 5 cut(s) 1053, 1140, 1371, 1850, 2621
BstDSI CCRYGG 2 cut(s) 525, 1006
BstEII GGTNACC 1 cut(s) 3728
BstENI CCTNNNNNAGG 1 cut(s) 3881
BstFNI CGCG 3 cut(s) 1053, 2619, 3807
BstH2I RGCGCY 2 cut(s) 756, 2500
BstHHI GCGC 4 cut(s) 755, 1053, 1055, 2499
BstMCI CGRYCG 1 cut(s) 3270
BstMWI GCNNNNNNNGC 9 cut(s) 91, 431, 1139, 1465, 1837, 1858, 2296, 2753, 3785
BstNI CCWGG 4 cut(s) 454, 1518, 2102, 2895
BstNSI RCATGY 3 cut(s) 1329, 1890, 3359
BstPAI GACNNNNGTC 1 cut(s) 419
BstPI GGTNACC 1 cut(s) 3728
BstSCI CCNGG 4 cut(s) 452, 1516, 2100, 2893
BstSFI CTRYAG 1 cut(s) 2413
BstSLI GKGCMC 2 cut(s) 853, 1006
BstUI CGCG 3 cut(s) 1053, 2619, 3807
BstV1I GCAGC 1 cut(s) 81
BstV2I GAAGAC 6 cut(s) 25, 1142, 1700, 2949, 3456, 3996
BstX2I RGATCY 3 cut(s) 310, 1939, 3260
BstYI RGATCY 3 cut(s) 310, 1939, 3260
BstZ17I GTATAC 2 cut(s) 3290, 3872
BsuI GTATCC 3 cut(s) 1603, 2644, 3182
BsuRI GGCC 3 cut(s) 1004, 1952, 3960
BtgI CCRYGG 2 cut(s) 525, 1006
BtgZI GCGATG 1 cut(s) 3515
BtrI CACGTC 1 cut(s) 1342
BtsI GCAGTG 2 cut(s) 3033, 3621
BtsIMutI CAGTG 9 cut(s) 851, 1400, 1506, 1573, 1787, 3033, 3621, 3922, 4057
Cac8I GCNNGC 5 cut(s) 1053, 1140, 1371, 1850, 2621
CfoI GCGC 4 cut(s) 755, 1053, 1055, 2499
Cfr13I GGNCC 2 cut(s) 1002, 1003
CseI GACGC 2 cut(s) 2791, 2818
Csp6I GTAC 8 cut(s) 917, 1204, 1493, 1557, 1562, 1581, 1637, 3345
CspCI CAANNNNNGTGG 4 cut(s) 1942, 1977, 2712, 2747
CviQI GTAC 8 cut(s) 917, 1204, 1493, 1557, 1562, 1581, 1637, 3345
DinI GGCGCC 1 cut(s) 754
DraI TTTAAA 3 cut(s) 2650, 3276, 3604
DrdI GACNNNNNNGTC 1 cut(s) 3848
DseDI GACNNNNNNGTC 1 cut(s) 3848
Ecl136II GAGCTC 3 cut(s) 665, 1117, 2332
Eco130I CCWWGG 5 cut(s) 1006, 1223, 2278, 2564, 3769
Eco24I GRGCYC 4 cut(s) 667, 1006, 1119, 2334
Eco31I GGTCTC 2 cut(s) 114, 2768
Eco32I GATATC 1 cut(s) 2509
Eco47III AGCGCT 1 cut(s) 2498
Eco53kI GAGCTC 3 cut(s) 665, 1117, 2332
Eco57I CTGAAG 4 cut(s) 28, 1122, 2992, 3580
Eco88I CYCGRG 2 cut(s) 37, 634
Eco91I GGTNACC 1 cut(s) 3728
EcoICRI GAGCTC 3 cut(s) 665, 1117, 2332
EcoNI CCTNNNNNAGG 1 cut(s) 3881
EcoO109I RGGNCCY 1 cut(s) 1002
EcoO65I GGTNACC 1 cut(s) 3728
EcoRI GAATTC 1 cut(s) 2150
EcoRII CCWGG 4 cut(s) 452, 1516, 2100, 2893
EcoRV GATATC 1 cut(s) 2509
EcoT14I CCWWGG 5 cut(s) 1006, 1223, 2278, 2564, 3769
EcoT22I ATGCAT 2 cut(s) 1842, 4019
EcoT38I GRGCYC 4 cut(s) 667, 1006, 1119, 2334
EgeI GGCGCC 1 cut(s) 754
EheI GGCGCC 1 cut(s) 754
ErhI CCWWGG 5 cut(s) 1006, 1223, 2278, 2564, 3769
FalI AAGNNNNNCTT 4 cut(s) 770, 802, 980, 1012
FaqI GGGAC 2 cut(s) 1408, 3406
FauI CCCGC 2 cut(s) 362, 2681
FbaI TGATCA 7 cut(s) 838, 1015, 1444, 3217, 3925, 3970, 4027
FblI GTMKAC 2 cut(s) 3289, 3871
Fnu4HI GCNGC 2 cut(s) 95, 2617
FriOI GRGCYC 4 cut(s) 667, 1006, 1119, 2334
Fsp4HI GCNGC 2 cut(s) 95, 2617
GlaI GCGC 4 cut(s) 754, 1052, 1054, 2498
GluI GCNGC 2 cut(s) 95, 2617
GsaI CCCAGC 1 cut(s) 438
GsuI CTGGAG 3 cut(s) 2008, 2444, 3768
HaeII RGCGCY 2 cut(s) 756, 2500
HaeIII GGCC 3 cut(s) 1004, 1952, 3960
HapII CCGG 3 cut(s) 761, 1730, 2439
HgaI GACGC 2 cut(s) 2791, 2818
HhaI GCGC 4 cut(s) 755, 1053, 1055, 2499
Hin1I GRCGYC 1 cut(s) 753
Hin6I GCGC 4 cut(s) 753, 1051, 1053, 2497
HinP1I GCGC 4 cut(s) 753, 1051, 1053, 2497
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HindIII AAGCTT 1 cut(s) 3606
HpaII CCGG 3 cut(s) 761, 1730, 2439
Hpy166II GTNNAC 8 cut(s) 414, 506, 851, 1506, 1637, 3290, 3440, 3872
Hpy8I GTNNAC 8 cut(s) 414, 506, 851, 1506, 1637, 3290, 3440, 3872
Hpy99I CGWCG 1 cut(s) 66
HpyCH4IV ACGT 5 cut(s) 1293, 1341, 2954, 3453, 3703
HpyF10VI GCNNNNNNNGC 9 cut(s) 91, 431, 1139, 1465, 1837, 1858, 2296, 2753, 3785
HpySE526I ACGT 5 cut(s) 1293, 1341, 2954, 3453, 3703
Hsp92I GRCGYC 1 cut(s) 753
HspAI GCGC 4 cut(s) 753, 1051, 1053, 2497
KasI GGCGCC 1 cut(s) 752
Kpn2I TCCGGA 1 cut(s) 760
Ksp22I TGATCA 7 cut(s) 838, 1015, 1444, 3217, 3925, 3970, 4027
LguI GCTCTTC 1 cut(s) 4032
LmnI GCTCC 5 cut(s) 960, 1114, 1871, 1999, 2329
Lsp1109I GCAGC 1 cut(s) 81
LweI GCATC 6 cut(s) 337, 1270, 1446, 1922, 2292, 2471
MaeII ACGT 5 cut(s) 1293, 1341, 2954, 3453, 3703
MaeIII GTNAC 7 cut(s) 338, 448, 2408, 3421, 3655, 3728, 3839
MfeI CAATTG 3 cut(s) 590, 1766, 2683
MflI RGATCY 3 cut(s) 310, 1939, 3260
MhlI GDGCHC 6 cut(s) 667, 853, 1006, 1119, 2334, 3755
Mly113I GGCGCC 1 cut(s) 753
MlyI GAGTC 5 cut(s) 2065, 2435, 2930, 3288, 3836
MmeI TCCRAC 4 cut(s) 44, 228, 2016, 2379
Mph1103I ATGCAT 2 cut(s) 1842, 4019
MroI TCCGGA 1 cut(s) 760
MroXI GAANNNNTTC 4 cut(s) 1649, 2291, 2559, 2956
MslI CAYNNNNRTG 6 cut(s) 846, 1644, 1792, 1885, 2300, 3360
MspA1I CMGCKG 4 cut(s) 48, 3317, 3476, 3788
MspCI CTTAAG 1 cut(s) 997
MspI CCGG 3 cut(s) 761, 1730, 2439
MspR9I CCNGG 4 cut(s) 454, 1518, 2102, 2895
MunI CAATTG 3 cut(s) 590, 1766, 2683
Mva1269I GAATGC 2 cut(s) 1651, 2129
MvaI CCWGG 4 cut(s) 454, 1518, 2102, 2895
MvnI CGCG 3 cut(s) 1053, 2619, 3807
MwoI GCNNNNNNNGC 9 cut(s) 91, 431, 1139, 1465, 1837, 1858, 2296, 2753, 3785
NarI GGCGCC 1 cut(s) 753
NcoI CCATGG 1 cut(s) 1006
NlaIV GGNNCC 7 cut(s) 233, 312, 631, 754, 1004, 2562, 3544
NmeAIII GCCGAG 1 cut(s) 664
NmuCI GTSAC 5 cut(s) 338, 448, 3421, 3655, 3839
NsiI ATGCAT 2 cut(s) 1842, 4019
NspI RCATGY 3 cut(s) 1329, 1890, 3359
NspV TTCGAA 1 cut(s) 1816
PauI GCGCGC 1 cut(s) 1051
PciI ACATGT 2 cut(s) 1886, 3355
PciSI GCTCTTC 1 cut(s) 4032
PcsI WCGNNNNNNNCGW 2 cut(s) 1830, 2457
PctI GAATGC 2 cut(s) 1651, 2129
PdmI GAANNNNTTC 4 cut(s) 1649, 2291, 2559, 2956
PflMI CCANNNNNTGG 1 cut(s) 130
PkrI GCNGC 2 cut(s) 96, 2618
PleI GAGTC 5 cut(s) 2065, 2434, 2930, 3288, 3836
PluTI GGCGCC 1 cut(s) 756
PpsI GAGTC 5 cut(s) 2065, 2434, 2930, 3288, 3836
Ppu21I YACGTR 1 cut(s) 3704
PscI ACATGT 2 cut(s) 1886, 3355
PshAI GACNNNNGTC 1 cut(s) 419
PshBI ATTAAT 1 cut(s) 3857
PsiI TTATAA 1 cut(s) 3714
Psp124BI GAGCTC 3 cut(s) 667, 1119, 2334
Psp6I CCWGG 4 cut(s) 452, 1516, 2100, 2893
PspEI GGTNACC 1 cut(s) 3728
PspFI CCCAGC 1 cut(s) 434
PspGI CCWGG 4 cut(s) 452, 1516, 2100, 2893
PspN4I GGNNCC 7 cut(s) 233, 312, 631, 754, 1004, 2562, 3544
PspOMI GGGCCC 1 cut(s) 1002
PspPI GGNCC 2 cut(s) 1002, 1003
PsrI GAACNNNNNNTAC 2 cut(s) 613, 645
PstI CTGCAG 1 cut(s) 2417
PsuI RGATCY 3 cut(s) 310, 1939, 3260
PteI GCGCGC 1 cut(s) 1051
PvuII CAGCTG 2 cut(s) 3476, 3788
RsaI GTAC 8 cut(s) 918, 1205, 1494, 1558, 1563, 1582, 1638, 3346
RsaNI GTAC 8 cut(s) 917, 1204, 1493, 1557, 1562, 1581, 1637, 3345
RseI CAYNNNNRTG 6 cut(s) 846, 1644, 1792, 1885, 2300, 3360
SacI GAGCTC 3 cut(s) 667, 1119, 2334
SapI GCTCTTC 1 cut(s) 4032
SatI GCNGC 2 cut(s) 95, 2617
Sau96I GGNCC 2 cut(s) 1002, 1003
ScaI AGTACT 2 cut(s) 1205, 3346
SchI GAGTC 5 cut(s) 2065, 2435, 2930, 3288, 3836
ScrFI CCNGG 4 cut(s) 454, 1518, 2102, 2895
SduI GDGCHC 6 cut(s) 667, 853, 1006, 1119, 2334, 3755
SfaNI GCATC 6 cut(s) 337, 1270, 1446, 1922, 2292, 2471
SfcI CTRYAG 1 cut(s) 2413
SfoI GGCGCC 1 cut(s) 754
SfuI TTCGAA 1 cut(s) 1816
SmiMI CAYNNNNRTG 6 cut(s) 846, 1644, 1792, 1885, 2300, 3360
SmlI CTYRAG 4 cut(s) 86, 997, 1056, 2390
SmoI CTYRAG 4 cut(s) 86, 997, 1056, 2390
SpeI ACTAGT 1 cut(s) 3245
SsiI CCGC 7 cut(s) 48, 369, 2058, 2255, 2617, 2688, 3317
SspDI GGCGCC 1 cut(s) 752
SspI AATATT 4 cut(s) 1252, 1396, 1489, 3235
SstI GAGCTC 3 cut(s) 667, 1119, 2334
StyD4I CCNGG 4 cut(s) 452, 1516, 2100, 2893
StyI CCWWGG 5 cut(s) 1006, 1223, 2278, 2564, 3769
TaiI ACGT 5 cut(s) 1296, 1344, 2957, 3456, 3706
TatI WGTACW 4 cut(s) 1203, 1561, 1636, 3344
TauI GCSGC 1 cut(s) 2619
TscAI CASTG 9 cut(s) 858, 1407, 1513, 1573, 1794, 3033, 3621, 3922, 4057
TseFI GTSAC 5 cut(s) 338, 448, 3421, 3655, 3839
TseI GCWGC 1 cut(s) 94
Tsp45I GTSAC 5 cut(s) 338, 448, 3421, 3655, 3839
TspGWI ACGGA 8 cut(s) 50, 94, 542, 1304, 1741, 2222, 3164, 3440
TspRI CASTG 9 cut(s) 858, 1407, 1513, 1573, 1794, 3033, 3621, 3922, 4057
Van91I CCANNNNNTGG 1 cut(s) 130
Vha464I CTTAAG 1 cut(s) 997
VneI GTGCAC 1 cut(s) 849
VspI ATTAAT 1 cut(s) 3857
XagI CCTNNNNNAGG 1 cut(s) 3881
XbaI TCTAGA 3 cut(s) 182, 1240, 2536
XceI RCATGY 3 cut(s) 1329, 1890, 3359
XmiI GTMKAC 2 cut(s) 3289, 3871
XmnI GAANNNNTTC 4 cut(s) 1649, 2291, 2559, 2956
ZrmI AGTACT 2 cut(s) 1205, 3346
Zsp2I ATGCAT 2 cut(s) 1842, 4019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.