AT5G46520

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002912
Physical Location & Seq
Forward (+)
18867616 .. 18872494
4879 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46520.2

Sequence Viewer

Length: 3507 bp
ATGGCTTCTTCATCTTCTTCTCGCAATTGGGTTTATGATGTTTTCTTGAGCTTCAGTGGGAAAGACGTCCGCGTAACATTCCGTAGCCACTTTCTCAAAGAGCTTGATCGGAAACTGATCTCTGCTTTCAGAGACAATGAGATCGAGAGAAGCCACTCTCTTTGGCCCGATCTTGAACAAGCGATCAAGGACTCCAGAATCGCAGTGGTCGTTTTCTCCAAAAACTACGCTTCGTCGAGCTGGTGTCTTAACGAGTTGCTGGAGATTGTGAACTGCAATGATAAAATTATCATACCGGTTTTCTACGGTGTGGATCCTTCCCAAGTGAGGTATCAAATCGGTGAATTTGGAAGTATCTTTGAAAAGACTTGCAAGAGACAAACAGAGGAAGTGAAAAATCAATGGAAGAAAGCGTTGACTGATGTAGCTAATATGCTTGGATTTGATTCTGCCAAATGGGATGACGAAGCAAAAATGATTGAAGAAATAGCCAATGATGTTTTGGCTAAACTGCTTTTAACTTCATCCACGGATTCTGCTGAGAACTCTATTGGCATTGAAGATCATATTGCAAATATGAGTGTATTGTTGAAATTGGAAGCTGAGGAAGTGAGAATGGTTGGTATATGGGGTTCCTCGGGGATTGGTAAGACTACCATTGCAAGAGCTCTGTTTAACCAACTTTCTCGGCATTTCCCAGTTAGCAAATTCATAGACAGAGCTTTTGTATATAAGAGTAGAGAAACTTACAAGGGCGCCAATCCGGACGACCCGAACATGAAGTTGCACTTGCAAGGATGTTTCCTATCGGAAATTTTGGGGAAAAAAGACATAAAGATTGATCATTTAGGTGCACTGGGAGAGAGGCTAAAGCACCAGAAAACTCTCATCATCATTGATGATTTGGATGATCTAGTGGTACTAGATTCATTGGTGGGTAAAACTAATTGGTTTGGATGTGGGAGCAGGATCATAGTGATTACCAATAATAAGCAATTCTTAAGGGCCCATGGAATTGATCATATTTACGAAGTTAGTCTCCCTTCTAAAGAGCGCGCTCAAGAGATGTTTTGTCAGTCTGCTTTCGGGGAAAACTCTCCACCTGAAGGTTTTGAGGAGCTCGTAGTTGAAATTGCATGGCTTGCTGGTAGTCTTCCTTTGGGTCTTACTGTTTTTGGTTCAGCTCTACGAGGGAGAAAAAAGGAGTACTGGGTGAAGATGTTGCCAAGGCTTCAAAATGATCTAGATGGCAATATTGAGGAGACATTGAAAGTCAGCTATGATGCGATAGGCAACGTAAAAGATCAAGCGTTATTCCGTCTTATCGCATGTCTTTTCAATCACGTCAAAGTCAGAGACATTGAATTGTTGCTCGCAGATAGTGGCTTAGATGTAAATATTGCACTGGAAAACCTAGTTGATAAGTCCCTTATTCATGTCAGAAATGATCATGTGGAGATGCACCGTTTGCTACAAGAAACGGGTAGAAATATTGTACGCTCACAGTCCACTGACAACCCAGGAGAACGAGAATTTCTGGTGGATTCAAACGATAGTCGTACTGTACTCAGTGAAGGCATTGGTACTCGAAAGGTGCTAGGAATATCATTGGATACAAGTAAGGTTAGTGAGTTTTGTGTACATGAGAATGCCTTCAAAGGGATGGGTAATCTCCTTTTCTTGGATATTTCCTCGAAGACTTTTATAGAAGAAGAAGTCAAAGTGCATTTACCGGAAAAAATAAACTATTATTCCGTTCAACCCAAACAATTGATTTGGGATAGATTTCCACTGAAATGTATGCCTTATACTTTTCTTCGAAACCTCGTCAAGCTCGAAATGCATGACAGCAAGCTAGAGAAGCTGTGGGAAGGAGCTATGTCATTTACATGTCTAAAGGAATTGGATATGTGGGCATCTAAATACCTGAAAGAAATCCCAGATCTTTCAAAGGCCACTAACATTGAGAAACTTGACTTTGGACATTGCTGGAGTTTGGTGGAGCTTCCTTCCTCTATACGAAATCTCAATAAACTGTTGGAATTGAATATGGAATACTGCGGTGAATTGGAGACTCTTCCTACGGGTTTCAACCTCAAATCCCTGGACTACCTCAATTTCAATGAATGCTGGAAGTTGAGGACTTTTCCTGAATTCGCAACCAACATCTCAAATCTCATTCTGGCAGAAACATCTATTGAAGAATACCCTTCCAATTTGTATTTCAAGAATGTCCGTGAGCTAAGCATGGGGAAAGCGGATAGTGATGAGAATAAATGCCAAGGAGTGAAGCCCTTCATGCCGATGCTGTCTCCCACTTTGACCCTCTTGGAGCTCTGGAATATCCCAAATTTAGTAGAGCTTTCGTCCTCATTTCAGAATCTCAATAACCTTGAGAGGTTGGACATTTGTTACTGCAGAAATCTGGAGTCTCTACCCACCGGAATCAACCTCGAATCTCTCGTTTCCCTTAATCTCTTTGGATGCTCACGGTTGAAGCGCTTTCCTGATATCTCAACCAACATCAAATATCTCGATCTAGACCAAACTGGGATTGAAGAGGTTCCTTGGCAGATCGAGAACTTCTTTAACCTCACTAAGCTAACTATGAAAGGTTGCCGCGAGCTAAAATGTGTATCCCTAAACATTTTTAAACTCAAACATCTTGGGGAAGTTAGCTTTTCCAATTGCGGGGCATTGACCAGAGTTGATTTGAGTTGCTATCCAAGTGGGGTGGAGATGATGAAGGCAGACAATGCTGACATAGTTTCTGAGGAGACCACCTCTTCTCTTCCTGATAGTTGCGTCCTCAATGTCAATTTCATGGATTGCGTCAATCTGGATCGAGAACCTGTCCTTCACCAACAGTCGATTATTTTCAACTCCATGATATTGCCAGGTGAAGAAGTGCCATCATATTTCACTTACCGAACATCTGACTCTCAACCATTTGGAACGTCTTCCTCTCTGCCCATCCCTCTACTTCCCACACAACTCTCTCAACCATTCTTCAGATTTAGGGTTTGTGCAGTGGTAAGTGCTTCCAATGGAGTATATATCGGGGTATATAGTCGGTTCAAAGGCAGAATTGGGAACAAATTTGATTCCTTTGGTGAGGTACATAATTTCATGGAAATTGAGAAGGGTATTCATCTGTGTATATTTGACTGTCGTATCCGTCTATACAAAGATAATGTTCCTCTATCTCAACTGAACTATGATCATGTGGATATAAATATTCATATAACTAGTGGAGATTGGAGATCTACGGTCGTTTTAAAAGAATGGGGTATACGACTCTTAGAGACCGGTTCATCAGCAGAGAACCGACTTGGTAATCCAAACAGTACTCTTCCACATGTTTCTCAAGCCGAAGAAGGCAATATGGGGTATTATACACATGTTCAAGGACTTGTTAATGAGATTGAAAATAGTGAAGACTCTGGAGATAACAATGTAGAGACTGAGAGAAGCAAGAAGCGCATGCGGCTACACCACTTCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1168

Amino Acids

132.79

Weight (kDa)

5.47

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 50 - 218 6.3e-54 TIR domain
TIR_2 PF13676 52 - 144 1.2e-11 TIR domain
NB-ARC PF00931 236 - 402 2.9e-09 NB-ARC domain
WHD_ROQ1 PF23282 470 - 541 2.6e-39 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 646 - 663 2.7e-07 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 69
AccB1I GGYRCC 1 cut(s) 755
AccI GTMKAC 1 cut(s) 3292
AccII CGCG 3 cut(s) 72, 1056, 2622
AccIII TCCGGA 1 cut(s) 763
AciI CCGC 6 cut(s) 70, 2061, 2258, 2620, 2691, 3487
AclWI GGATC 4 cut(s) 308, 321, 977, 2850
AcsI RAATTY 7 cut(s) 344, 707, 813, 1532, 2153, 2350, 3098
AcuI CTGAAG 3 cut(s) 37, 1125, 2995
AcyI GRCGYC 2 cut(s) 66, 756
AfaI GTAC 9 cut(s) 921, 1208, 1497, 1561, 1566, 1585, 1641, 3120, 3349
AfeI AGCGCT 1 cut(s) 2501
AfiI CCNNNNNNNGG 5 cut(s) 327, 1106, 1659, 1681, 2691
AflII CTTAAG 1 cut(s) 1000
AflIII ACRYGT 3 cut(s) 1889, 3358, 3400
AgeI ACCGGT 2 cut(s) 295, 3308
AhlI ACTAGT 1 cut(s) 3248
AjiI CACGTC 1 cut(s) 1345
AjnI CCWGG 3 cut(s) 1519, 2103, 2896
AjuI GAANNNNNNNTTGG 2 cut(s) 2207, 2239
Alw21I GWGCWC 4 cut(s) 670, 856, 1122, 2337
Alw44I GTGCAC 1 cut(s) 852
AlwI GGATC 4 cut(s) 308, 321, 977, 2850
Ama87I CYCGRG 1 cut(s) 637
Aor13HI TCCGGA 1 cut(s) 763
Aor51HI AGCGCT 1 cut(s) 2501
AoxI GGCC 3 cut(s) 164, 1005, 1953
ApaI GGGCCC 1 cut(s) 1009
ApaLI GTGCAC 1 cut(s) 852
ApoI RAATTY 7 cut(s) 344, 707, 813, 1532, 2153, 2350, 3098
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
AsiGI ACCGGT 2 cut(s) 295, 3308
Asp700I GAANNNNTTC 4 cut(s) 1652, 2294, 2562, 2959
AspLEI GCGC 5 cut(s) 758, 1056, 1058, 2502, 3483
AspS9I GGNCC 3 cut(s) 165, 1005, 1006
AsuHPI GGTGA 6 cut(s) 353, 1225, 2075, 2852, 2912, 3125
AsuII TTCGAA 1 cut(s) 1819
AvaI CYCGRG 1 cut(s) 637
BaeGI GKGCMC 2 cut(s) 856, 1009
BaeI ACNNNNGTAYC 2 cut(s) 3157, 3190
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 4 cut(s) 670, 1009, 1122, 2337
BbsI GAAGAC 4 cut(s) 1145, 1703, 2952, 3444
Bbv12I GWGCWC 4 cut(s) 670, 856, 1122, 2337
BbvCI CCTCAGC 1 cut(s) 603
BccI CCATC 4 cut(s) 1241, 1657, 2920, 2981
BciT130I CCWGG 3 cut(s) 1521, 2105, 2898
BciVI GTATCC 3 cut(s) 1606, 2647, 3185
BclI TGATCA 4 cut(s) 841, 1018, 1447, 3220
BcuI ACTAGT 1 cut(s) 3248
BfaI CTAG 8 cut(s) 914, 923, 1244, 1415, 1598, 1856, 2540, 3249
BfmI CTRYAG 1 cut(s) 2416
BfoI RGCGCY 2 cut(s) 759, 2503
BfrI CTTAAG 1 cut(s) 1000
BfuI GTATCC 3 cut(s) 1606, 2647, 3185
BglII AGATCT 2 cut(s) 1942, 3263
BisI GCNGC 2 cut(s) 2620, 3488
BlpI GCTNAGC 1 cut(s) 2243
BlsI GCNGC 2 cut(s) 2621, 3489
BmcAI AGTACT 2 cut(s) 1208, 3349
Bme1390I CCNGG 3 cut(s) 1521, 2105, 2898
BmeT110I CYCGRG 1 cut(s) 637
BmgBI CACGTC 1 cut(s) 1345
BmgT120I GGNCC 3 cut(s) 165, 1005, 1006
BmiI GGNNCC 5 cut(s) 315, 634, 757, 1007, 2565
BmrFI CCNGG 3 cut(s) 1521, 2105, 2898
BmrI ACTGGG 4 cut(s) 692, 866, 1219, 2559
BmsI GCATC 5 cut(s) 1273, 1449, 1925, 2295, 2474
BmuI ACTGGG 4 cut(s) 692, 866, 1219, 2559
BpiI GAAGAC 4 cut(s) 1145, 1703, 2952, 3444
BplI GAGNNNNNCTC 2 cut(s) 2768, 2800
BpmI CTGGAG 5 cut(s) 178, 281, 2011, 2447, 3465
Bpu10I CCTNAGC 1 cut(s) 603
Bpu1102I GCTNAGC 1 cut(s) 2243
Bpu14I TTCGAA 1 cut(s) 1819
BpuEI CTTGAG 4 cut(s) 67, 1044, 2414, 3351
BsaHI GRCGYC 2 cut(s) 66, 756
BsaI GGTCTC 2 cut(s) 2771, 3299
BsaJI CCNNGG 8 cut(s) 528, 636, 1009, 1226, 1519, 2103, 2281, 2567
BsaWI WCCGGW 5 cut(s) 295, 763, 1732, 2441, 3308
Bsc4I CCNNNNNNNGG 5 cut(s) 327, 1106, 1659, 1681, 2691
Bse118I RCCGGY 2 cut(s) 295, 3308
Bse1I ACTGG 5 cut(s) 698, 861, 1214, 1410, 2554
Bse3DI GCAATG 3 cut(s) 283, 657, 1984
BseAI TCCGGA 1 cut(s) 763
BseBI CCWGG 3 cut(s) 1521, 2105, 2898
BseDI CCNNGG 8 cut(s) 528, 636, 1009, 1226, 1519, 2103, 2281, 2567
BseGI GGATG 8 cut(s) 466, 524, 803, 913, 962, 1668, 2489, 2973
BseLI CCNNNNNNNGG 5 cut(s) 327, 1106, 1659, 1681, 2691
BseMI GCAATG 3 cut(s) 283, 657, 1984
BseMII CTCAG 5 cut(s) 531, 594, 1582, 2763, 3456
BseNI ACTGG 5 cut(s) 698, 861, 1214, 1410, 2554
BsePI GCGCGC 1 cut(s) 1054
BseRI GAGGAG 3 cut(s) 1130, 1274, 2789
BseSI GKGCMC 2 cut(s) 856, 1009
BsgI GTGCAG 1 cut(s) 3048
Bsh1236I CGCG 3 cut(s) 72, 1056, 2622
Bsh1285I CGRYCG 1 cut(s) 3273
BshFI GGCC 3 cut(s) 166, 1007, 1955
BshNI GGYRCC 1 cut(s) 755
BshTI ACCGGT 2 cut(s) 295, 3308
BsiEI CGRYCG 1 cut(s) 3273
BsiHKAI GWGCWC 4 cut(s) 670, 856, 1122, 2337
BsiHKCI CYCGRG 1 cut(s) 637
BsiSI CCGG 5 cut(s) 296, 764, 1733, 2442, 3309
BslFI GGGAC 1 cut(s) 1411
BslI CCNNNNNNNGG 5 cut(s) 327, 1106, 1659, 1681, 2691
BsmFI GGGAC 1 cut(s) 1411
BsmI GAATGC 2 cut(s) 1654, 2132
BsnI GGCC 3 cut(s) 166, 1007, 1955
Bso31I GGTCTC 2 cut(s) 2771, 3299
BsoBI CYCGRG 1 cut(s) 637
Bsp119I TTCGAA 1 cut(s) 1819
Bsp120I GGGCCC 1 cut(s) 1005
Bsp1286I GDGCHC 5 cut(s) 670, 856, 1009, 1122, 2337
Bsp13I TCCGGA 1 cut(s) 763
Bsp1407I TGTACA 1 cut(s) 1639
Bsp1720I GCTNAGC 1 cut(s) 2243
Bsp19I CCATGG 1 cut(s) 1009
BspACI CCGC 6 cut(s) 70, 2061, 2258, 2620, 2691, 3487
BspANI GGCC 3 cut(s) 166, 1007, 1955
BspCNI CTCAG 5 cut(s) 532, 595, 1581, 2764, 3457
BspEI TCCGGA 1 cut(s) 763
BspFNI CGCG 3 cut(s) 72, 1056, 2622
BspLI GGNNCC 5 cut(s) 315, 634, 757, 1007, 2565
BspMAI CTGCAG 1 cut(s) 2420
BspPI GGATC 4 cut(s) 308, 321, 977, 2850
BspT104I TTCGAA 1 cut(s) 1819
BspT107I GGYRCC 1 cut(s) 755
BspTI CTTAAG 1 cut(s) 1000
BspTNI GGTCTC 2 cut(s) 2771, 3299
BsrDI GCAATG 3 cut(s) 283, 657, 1984
BsrFI RCCGGY 2 cut(s) 295, 3308
BsrGI TGTACA 1 cut(s) 1639
BsrI ACTGG 5 cut(s) 698, 861, 1214, 1410, 2554
BssAI RCCGGY 2 cut(s) 295, 3308
BssECI CCNNGG 8 cut(s) 528, 636, 1009, 1226, 1519, 2103, 2281, 2567
BssHII GCGCGC 1 cut(s) 1054
BssNAI GTATAC 1 cut(s) 3293
BssNI GRCGYC 2 cut(s) 66, 756
BssT1I CCWWGG 4 cut(s) 1009, 1226, 2281, 2567
Bst1107I GTATAC 1 cut(s) 3293
Bst2UI CCWGG 3 cut(s) 1521, 2105, 2898
Bst6I CTCTTC 5 cut(s) 2082, 2553, 2791, 2796, 3357
BstACI GRCGYC 2 cut(s) 66, 756
BstAFI CTTAAG 1 cut(s) 1000
BstAPI GCANNNNNTGC 3 cut(s) 1142, 1468, 2756
BstAUI TGTACA 1 cut(s) 1639
BstBI TTCGAA 1 cut(s) 1819
BstC8I GCNNGC 6 cut(s) 1056, 1143, 1374, 1853, 2624, 3485
BstDEI CTNAG 9 cut(s) 540, 603, 1387, 1568, 2243, 2598, 2772, 3301, 3465
BstDSI CCRYGG 2 cut(s) 528, 1009
BstF5I GGATG 8 cut(s) 466, 524, 803, 913, 962, 1668, 2489, 2973
BstFNI CGCG 3 cut(s) 72, 1056, 2622
BstH2I RGCGCY 2 cut(s) 759, 2503
BstHHI GCGC 5 cut(s) 758, 1056, 1058, 2502, 3483
BstMCI CGRYCG 1 cut(s) 3273
BstMWI GCNNNNNNNGC 8 cut(s) 1142, 1468, 1840, 1861, 2299, 2756, 3480, 3487
BstNI CCWGG 3 cut(s) 1521, 2105, 2898
BstNSI RCATGY 5 cut(s) 1332, 1893, 3362, 3404, 3487
BstSCI CCNGG 3 cut(s) 1519, 2103, 2896
BstSFI CTRYAG 1 cut(s) 2416
BstSLI GKGCMC 2 cut(s) 856, 1009
BstUI CGCG 3 cut(s) 72, 1056, 2622
BstV2I GAAGAC 4 cut(s) 1145, 1703, 2952, 3444
BstX2I RGATCY 3 cut(s) 313, 1942, 3263
BstYI RGATCY 3 cut(s) 313, 1942, 3263
BstZ17I GTATAC 1 cut(s) 3293
BsuI GTATCC 3 cut(s) 1606, 2647, 3185
BsuRI GGCC 3 cut(s) 166, 1007, 1955
BtgI CCRYGG 2 cut(s) 528, 1009
BtrI CACGTC 1 cut(s) 1345
BtsCI GGATG 8 cut(s) 466, 524, 803, 913, 962, 1668, 2489, 2973
BtsI GCAGTG 2 cut(s) 210, 3036
BtsIMutI CAGTG 8 cut(s) 61, 210, 854, 1403, 1509, 1576, 1790, 3036
Cac8I GCNNGC 6 cut(s) 1056, 1143, 1374, 1853, 2624, 3485
CfoI GCGC 5 cut(s) 758, 1056, 1058, 2502, 3483
Cfr10I RCCGGY 2 cut(s) 295, 3308
Cfr13I GGNCC 3 cut(s) 165, 1005, 1006
CseI GACGC 2 cut(s) 2794, 2821
Csp6I GTAC 9 cut(s) 920, 1207, 1496, 1560, 1565, 1584, 1640, 3119, 3348
CspAI ACCGGT 2 cut(s) 295, 3308
CspCI CAANNNNNGTGG 6 cut(s) 143, 178, 1945, 1980, 2715, 2750
CviQI GTAC 9 cut(s) 920, 1207, 1496, 1560, 1565, 1584, 1640, 3119, 3348
DdeI CTNAG 9 cut(s) 540, 603, 1387, 1568, 2243, 2598, 2772, 3301, 3465
DinI GGCGCC 1 cut(s) 757
DraI TTTAAA 2 cut(s) 2653, 3279
Eam1104I CTCTTC 5 cut(s) 2082, 2553, 2791, 2796, 3357
EarI CTCTTC 5 cut(s) 2082, 2553, 2791, 2796, 3357
Ecl136II GAGCTC 3 cut(s) 668, 1120, 2335
Eco130I CCWWGG 4 cut(s) 1009, 1226, 2281, 2567
Eco24I GRGCYC 4 cut(s) 670, 1009, 1122, 2337
Eco31I GGTCTC 2 cut(s) 2771, 3299
Eco32I GATATC 1 cut(s) 2512
Eco47III AGCGCT 1 cut(s) 2501
Eco53kI GAGCTC 3 cut(s) 668, 1120, 2335
Eco57I CTGAAG 3 cut(s) 37, 1125, 2995
Eco88I CYCGRG 1 cut(s) 637
EcoICRI GAGCTC 3 cut(s) 668, 1120, 2335
EcoO109I RGGNCCY 1 cut(s) 1005
EcoRI GAATTC 1 cut(s) 2153
EcoRII CCWGG 3 cut(s) 1519, 2103, 2896
EcoRV GATATC 1 cut(s) 2512
EcoT14I CCWWGG 4 cut(s) 1009, 1226, 2281, 2567
EcoT22I ATGCAT 1 cut(s) 1845
EcoT38I GRGCYC 4 cut(s) 670, 1009, 1122, 2337
EgeI GGCGCC 1 cut(s) 757
EheI GGCGCC 1 cut(s) 757
ErhI CCWWGG 4 cut(s) 1009, 1226, 2281, 2567
FalI AAGNNNNNCTT 4 cut(s) 773, 805, 983, 1015
FaqI GGGAC 1 cut(s) 1411
FauI CCCGC 1 cut(s) 2684
FauNDI CATATG 1 cut(s) 3503
FbaI TGATCA 4 cut(s) 841, 1018, 1447, 3220
FblI GTMKAC 1 cut(s) 3292
Fnu4HI GCNGC 2 cut(s) 2620, 3488
FokI GGATG 8 cut(s) 473, 511, 810, 920, 969, 1675, 2496, 2960
FriOI GRGCYC 4 cut(s) 670, 1009, 1122, 2337
Fsp4HI GCNGC 2 cut(s) 2620, 3488
FspBI CTAG 8 cut(s) 914, 923, 1244, 1415, 1598, 1856, 2540, 3249
GlaI GCGC 5 cut(s) 757, 1055, 1057, 2501, 3482
GluI GCNGC 2 cut(s) 2620, 3488
GsuI CTGGAG 5 cut(s) 178, 281, 2011, 2447, 3465
HaeII RGCGCY 2 cut(s) 759, 2503
HaeIII GGCC 3 cut(s) 166, 1007, 1955
HapII CCGG 5 cut(s) 296, 764, 1733, 2442, 3309
HgaI GACGC 2 cut(s) 2794, 2821
HhaI GCGC 5 cut(s) 758, 1056, 1058, 2502, 3483
Hin1I GRCGYC 2 cut(s) 66, 756
Hin6I GCGC 5 cut(s) 756, 1054, 1056, 2500, 3481
HinP1I GCGC 5 cut(s) 756, 1054, 1056, 2500, 3481
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HpaII CCGG 5 cut(s) 296, 764, 1733, 2442, 3309
HphI GGTGA 6 cut(s) 353, 1225, 2075, 2852, 2912, 3125
Hpy166II GTNNAC 6 cut(s) 271, 417, 854, 1509, 1640, 3293
Hpy188I TCNGA 9 cut(s) 111, 131, 811, 1355, 1442, 2379, 2773, 2938, 3014
Hpy8I GTNNAC 6 cut(s) 271, 417, 854, 1509, 1640, 3293
Hpy99I CGWCG 1 cut(s) 238
HpyCH4IV ACGT 4 cut(s) 66, 1296, 1344, 2957
HpyF10VI GCNNNNNNNGC 8 cut(s) 1142, 1468, 1840, 1861, 2299, 2756, 3480, 3487
HpyF3I CTNAG 9 cut(s) 540, 603, 1387, 1568, 2243, 2598, 2772, 3301, 3465
HpySE526I ACGT 4 cut(s) 66, 1296, 1344, 2957
Hsp92I GRCGYC 2 cut(s) 66, 756
HspAI GCGC 5 cut(s) 756, 1054, 1056, 2500, 3481
KasI GGCGCC 1 cut(s) 755
Kpn2I TCCGGA 1 cut(s) 763
Ksp22I TGATCA 4 cut(s) 841, 1018, 1447, 3220
LmnI GCTCC 5 cut(s) 963, 1117, 1874, 2002, 2332
LweI GCATC 5 cut(s) 1273, 1449, 1925, 2295, 2474
MaeI CTAG 8 cut(s) 914, 923, 1244, 1415, 1598, 1856, 2540, 3249
MaeII ACGT 4 cut(s) 66, 1296, 1344, 2957
MaeIII GTNAC 2 cut(s) 73, 2411
MfeI CAATTG 3 cut(s) 25, 1769, 2686
MflI RGATCY 3 cut(s) 313, 1942, 3263
MhlI GDGCHC 5 cut(s) 670, 856, 1009, 1122, 2337
Mly113I GGCGCC 1 cut(s) 756
MlyI GAGTC 6 cut(s) 185, 2068, 2438, 2933, 3291, 3434
MmeI TCCRAC 2 cut(s) 2019, 2382
Mph1103I ATGCAT 1 cut(s) 1845
MroI TCCGGA 1 cut(s) 763
MroXI GAANNNNTTC 4 cut(s) 1652, 2294, 2562, 2959
MseI TTAA 9 cut(s) 249, 518, 675, 1001, 2472, 2589, 2652, 3278, 3417
MslI CAYNNNNRTG 5 cut(s) 849, 1647, 1795, 1888, 2303
MspCI CTTAAG 1 cut(s) 1000
MspI CCGG 5 cut(s) 296, 764, 1733, 2442, 3309
MspR9I CCNGG 3 cut(s) 1521, 2105, 2898
MunI CAATTG 3 cut(s) 25, 1769, 2686
Mva1269I GAATGC 2 cut(s) 1654, 2132
MvaI CCWGG 3 cut(s) 1521, 2105, 2898
MvnI CGCG 3 cut(s) 72, 1056, 2622
MwoI GCNNNNNNNGC 8 cut(s) 1142, 1468, 1840, 1861, 2299, 2756, 3480, 3487
NarI GGCGCC 1 cut(s) 756
NcoI CCATGG 1 cut(s) 1009
NdeI CATATG 1 cut(s) 3503
NlaIV GGNNCC 5 cut(s) 315, 634, 757, 1007, 2565
NmeAIII GCCGAG 1 cut(s) 667
NsiI ATGCAT 1 cut(s) 1845
NspI RCATGY 5 cut(s) 1332, 1893, 3362, 3404, 3487
NspV TTCGAA 1 cut(s) 1819
PaeI GCATGC 1 cut(s) 3487
PauI GCGCGC 1 cut(s) 1054
PciI ACATGT 3 cut(s) 1889, 3358, 3400
PcsI WCGNNNNNNNCGW 3 cut(s) 207, 1833, 2460
PctI GAATGC 2 cut(s) 1654, 2132
PdmI GAANNNNTTC 4 cut(s) 1652, 2294, 2562, 2959
PfeI GAWTC 9 cut(s) 198, 446, 533, 926, 1544, 2380, 2445, 2456, 3104
PinAI ACCGGT 2 cut(s) 295, 3308
PkrI GCNGC 2 cut(s) 2621, 3489
PleI GAGTC 6 cut(s) 185, 2068, 2437, 2933, 3291, 3434
PluTI GGCGCC 1 cut(s) 759
PpsI GAGTC 6 cut(s) 185, 2068, 2437, 2933, 3291, 3434
PscI ACATGT 3 cut(s) 1889, 3358, 3400
Psp124BI GAGCTC 3 cut(s) 670, 1122, 2337
Psp6I CCWGG 3 cut(s) 1519, 2103, 2896
PspGI CCWGG 3 cut(s) 1519, 2103, 2896
PspN4I GGNNCC 5 cut(s) 315, 634, 757, 1007, 2565
PspOMI GGGCCC 1 cut(s) 1005
PspPI GGNCC 3 cut(s) 165, 1005, 1006
PsrI GAACNNNNNNTAC 2 cut(s) 616, 648
PstI CTGCAG 1 cut(s) 2420
PsuI RGATCY 3 cut(s) 313, 1942, 3263
PteI GCGCGC 1 cut(s) 1054
RsaI GTAC 9 cut(s) 921, 1208, 1497, 1561, 1566, 1585, 1641, 3120, 3349
RsaNI GTAC 9 cut(s) 920, 1207, 1496, 1560, 1565, 1584, 1640, 3119, 3348
RseI CAYNNNNRTG 5 cut(s) 849, 1647, 1795, 1888, 2303
SacI GAGCTC 3 cut(s) 670, 1122, 2337
SaqAI TTAA 9 cut(s) 249, 518, 675, 1001, 2472, 2589, 2652, 3278, 3417
SatI GCNGC 2 cut(s) 2620, 3488
Sau96I GGNCC 3 cut(s) 165, 1005, 1006
ScaI AGTACT 2 cut(s) 1208, 3349
SchI GAGTC 6 cut(s) 185, 2068, 2438, 2933, 3291, 3434
ScrFI CCNGG 3 cut(s) 1521, 2105, 2898
SduI GDGCHC 5 cut(s) 670, 856, 1009, 1122, 2337
SfaNI GCATC 5 cut(s) 1273, 1449, 1925, 2295, 2474
SfcI CTRYAG 1 cut(s) 2416
SfoI GGCGCC 1 cut(s) 757
SfuI TTCGAA 1 cut(s) 1819
SmiMI CAYNNNNRTG 5 cut(s) 849, 1647, 1795, 1888, 2303
SmlI CTYRAG 5 cut(s) 46, 1000, 1059, 2393, 3366
SmoI CTYRAG 5 cut(s) 46, 1000, 1059, 2393, 3366
SpeI ACTAGT 1 cut(s) 3248
SphI GCATGC 1 cut(s) 3487
SsiI CCGC 6 cut(s) 70, 2061, 2258, 2620, 2691, 3487
SspDI GGCGCC 1 cut(s) 755
SspI AATATT 4 cut(s) 1255, 1399, 1492, 3238
SspMI CTAG 8 cut(s) 914, 923, 1244, 1415, 1598, 1856, 2540, 3249
SstI GAGCTC 3 cut(s) 670, 1122, 2337
StyD4I CCNGG 3 cut(s) 1519, 2103, 2896
StyI CCWWGG 4 cut(s) 1009, 1226, 2281, 2567
TaiI ACGT 4 cut(s) 69, 1299, 1347, 2960
TatI WGTACW 4 cut(s) 1206, 1564, 1639, 3347
TauI GCSGC 2 cut(s) 2622, 3490
TfiI GAWTC 9 cut(s) 198, 446, 533, 926, 1544, 2380, 2445, 2456, 3104
Tru1I TTAA 9 cut(s) 249, 518, 675, 1001, 2472, 2589, 2652, 3278, 3417
Tru9I TTAA 9 cut(s) 249, 518, 675, 1001, 2472, 2589, 2652, 3278, 3417
TscAI CASTG 8 cut(s) 61, 210, 861, 1410, 1516, 1576, 1797, 3036
TspGWI ACGGA 6 cut(s) 71, 545, 1307, 1744, 2225, 3167
TspRI CASTG 8 cut(s) 61, 210, 861, 1410, 1516, 1576, 1797, 3036
Vha464I CTTAAG 1 cut(s) 1000
VneI GTGCAC 1 cut(s) 852
XapI RAATTY 7 cut(s) 344, 707, 813, 1532, 2153, 2350, 3098
XbaI TCTAGA 2 cut(s) 1243, 2539
XceI RCATGY 5 cut(s) 1332, 1893, 3362, 3404, 3487
XcmI CCANNNNNNNNNTGG 2 cut(s) 202, 499
XmiI GTMKAC 1 cut(s) 3292
XmnI GAANNNNTTC 4 cut(s) 1652, 2294, 2562, 2959
XspI CTAG 8 cut(s) 914, 923, 1244, 1415, 1598, 1856, 2540, 3249
ZraI GACGTC 1 cut(s) 67
ZrmI AGTACT 2 cut(s) 1208, 3349
Zsp2I ATGCAT 1 cut(s) 1845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.