AT5G22690

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002912
Physical Location & Seq
Forward (+)
7541072 .. 7545256
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22690.1

Sequence Viewer

Length: 3027 bp
ATGGCTCTTTCTTATCGCAATTGGTTGTACGACGTGTTCCCGAGCTTCAGCGGAGAAGACGTACGTGTAACTTTCCTCAGCCACTTTCTGAAGGAGCTTGATCGGAAATTGATTAGTGTATTCAAAGACAACGATATCCAGAGAAGTCAATCGCTCGATCCCGAGCTTAAACTGGCGATTAGGGATTCGAGGATTGCAATCGTCGTCTTCTCCAAAAACTACGCTGCTTCTAGCTGGTGCCTTGACGAGTTGCTGGAGATTGTGAAATGTAAAGAAGAGTTTGGTCAAATTGTAATTCCCGTTTTCTACGGTTTGGATCCTTGTCATGTTAGGAAACAGAGTGGCGAATTCGGAATTGTGTTTGAAAACACTTGCCAGACCAAAACAGATGATGAGATACAAAAATGGCGGAGAGCATTGACTGATGTAGCTAACATCCTCGGGTTTCATTCTTCCAACTGGGATAATGAAGCAACCATGGTTGAAGATATAGCCAATGATGTTTTGGCTAAACTGAATTTAACTACAACATCTAATGACTTTGAGGGTTTTGTTGGCATCGAAGGTCATATCGCAAAGATTAGTTTAATGTTGTGCTTGGAATGTAAACAAGTGAGGATGTTTGGGATTTGGGGACCTTCTGGTATTGGAAAGACTACCATTGCTAGAGCTCTGTTTAGTCGAATCTCTCGTCATTTCCAAGGTAGCGTTTTCTTAGACAGAGCTTTCGTGTCTAAGAGTATGGAAATTTATAGTGGAGGCAACGTGGACAACTACAACGCCAAGCTGCATTTGCAAGGAAAGTTTTTGTCTGAAATTTTAAGAGCAAAAGACATAAAGATATCTAATTTAGGTGTTGTGGGAGAGAGGCTAAAGCATATGAAAGTTTTAATCTTTATTGATGATTTGGATGATCAAGTGGTGTTAGATGCTTTGGCGAGTAAACCTCATTGGTTTGGTTGTGGGAGCAGAATCATTGTTATTACAAAAGATAAGCAATTTTTTAGGGCTCATGGGATTGGTCTTTTTTACGAAGTGGGTCTCCCATCTGATAAACTAGCTCTAGAGATGTTCTCTCAGTCTGCTTTCAGGCAAAACTCTCCACCTCCTGGTTTCACAGAGCTTGCTTCTGAAGTTTCAAAACGTTCTGGTAATCTTCCTTTGGCTCTTAATGTTTTGGGTTCGCATCTTCGAGGGAGGGACAAAGAAGATTGGATTGATATGTTGCCAAGGCTTCGAAAAGGTTTGGATGGGAAGATTGAGAAAATATTAAGAGTTGGCTACGATGAGTTAAGTAATAAAGATGATAAAGCTATATTTCGTCTCATCGCATGTCTCTTCAATGGTGCGGAAATCAGTTACATCAAGCTGCTACTTGCAGATAGTAACTTGGGTGTTACCATTGGGCTTAAAAACCTAGTTGATAAGTCCCTCATACGTATTGGGTGTGACACTGTGGAGATGCACAGCATGTTACAAGAAATGGGTAGAGAAATTGTTCGGGAACAGTCCATTTACGAGCCTGGAGAACGGGAATTTCTGGTGGATTCGACGGATATTTTGGATGTACTTAATGATAACACTGGTACGAAAAAGGTTTTAGGTATATCATTTGATATGTCTGAGATTGAGGAGTTGCACATACATAAGAGAGCCTTCAAAAGGATGCCTAATCTCAGGTTCTTAAGATTTTACAAGAAATTAGGGAAGCAGAGTAAAGAAGCCAGATTGCACCTACAAGAAGGCTTTGACAAGTTTTTCCCCCCTAAACTCAAATTACTGAGTTGGGATGACTACCCGATGAGGCGTATGCCTTCTAACTTTCATGCTGGATATCTCGTTGTACTGAGAATGCAACACAGCAAGCTCGAAAAGTTATGGCAAGGAGTTCAGCCGCTTACATGCCTCAGGGAGATGCAGTTGTGGGGATCAAAAAAACTGAAAGAAATCCCAGATCTCTCGTTGGCCACCAATCTTGAAACACTTTATCTCAACGATTGCTCGAGTTTAGTGGAGCTTCCTTCCTCTATCAAGAATCTGAATAAACTGTGGGACTTGGGTATGAAAGGATGTGAAAAGCTGGAGCTTCTTCCAACCGACATCAACCTCAAATCTCTCTATCGCCTTGATCTTGGTAGATGCTCACGGTTGAAGAGTTTTCCTGATATCTCAAGTAACATTTCAGAGCTCTATCTGAATCGAACAGCGATTGAAGAAGTTCCTTGGTGGATCCAGAAATTCTCCAGGCTTAAACGTTTACGTATGAGGGAATGCAAAAAGCTCAAATGTATATCTCCAAACATCTCCAAACTGAAACATCTTGAGATGCTCGACTTTTCAAACTGCATAGCAACTACAGAAGAAGAAGCTCTTGTTCAACAACAATCAGTTTTGAAGTATCTCATATTTCCAGGAGGACAAGTGCCTTTGTATTTCACTTACCAAGCTACTGGAAGCTCTTTGGCCATCCCTTTGTCATTACATCAGAGCTCTCTCTCTCAACAATTATTGGGATTTAGGGCTTGCGTCGTGCTTGATGCTGAATCTATGTCCTCCGAGTTATACGTAATTGATATCAAGGTATGTTGTCGCTTGAGTGGTAAACGCAGCAACCTCTTTGATTCCGCTGACTGTCGGGATGCCTTCTTTACACCTCAGATGGATAGTCATTTGGTTATATTTGATTGTTGTTTCCCTCTAAACCAAGATAACGTTCGTCTAGCTGAACTGAACAATGATAAGGTGGTCACAGAGTTTCATTTCACTAGTATTTCTCGCTGCAAAATAACAGGAGTCGGTGTACGCTTCCTCAGGGACTGTTCATTACCAGAGAACCATCATAATGATCCAAATATTCTTGCACCTAATTGTGGATGCCCTGAGACTGAACACTCTGATGAGTATGGAGAATTCGGTGTAGAGACGAAAAGAAGTAGGAAGCGAAAGCGGATAACCTTAAGGACCGCTCAAGAAACTATTGACTTGCCCTACCTTCAGATCCGGGAAGAACAGAGGATGTCACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1008

Amino Acids

115.16

Weight (kDa)

8.0

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 10 - 187 6.3e-61 TIR domain
TIR_2 PF13676 12 - 107 5.8e-12 TIR domain
NB-ARC PF00931 190 - 366 5.7e-07 NB-ARC domain
WHD_ROQ1 PF23282 433 - 504 2.6e-28 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 612 - 631 1e-07 Leucine Rich Repeat
C-JID PF20160 803 - 885 4.8e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 237
AccB7I CCANNNNNTGG 1 cut(s) 1109
AccBSI CCGCTC 1 cut(s) 2966
AciI CCGC 7 cut(s) 51, 409, 1349, 1895, 2625, 2947, 2964
AclI AACGTT 3 cut(s) 1144, 2254, 2712
AclWI GGATC 8 cut(s) 152, 311, 324, 1936, 2224, 2237, 2840, 2992
AcoI YGGCCR 2 cut(s) 1965, 2463
AcsI RAATTY 7 cut(s) 347, 517, 747, 816, 1535, 2237, 2909
AcuI CTGAAG 4 cut(s) 31, 110, 1152, 2978
AfaI GTAC 6 cut(s) 29, 63, 1569, 1588, 1845, 2802
AfiI CCNNNNNNNGG 3 cut(s) 1109, 1662, 2870
AflII CTTAAG 2 cut(s) 1684, 2956
AflIII ACRYGT 2 cut(s) 33, 64
AhlI ACTAGT 1 cut(s) 2765
AjiI CACGTC 1 cut(s) 34
AjnI CCWGG 4 cut(s) 1108, 1522, 2243, 2410
AjuI GAANNNNNNNTTGG 2 cut(s) 2697, 2729
Alw21I GWGCWC 3 cut(s) 673, 2190, 2492
Alw26I GTCTC 5 cut(s) 1046, 1328, 1340, 2876, 2915
AlwI GGATC 8 cut(s) 152, 311, 324, 1936, 2224, 2237, 2840, 2992
AlwNI CAGNNNCTG 1 cut(s) 2817
Ama87I CYCGRG 4 cut(s) 40, 161, 440, 2002
AoxI GGCC 2 cut(s) 1965, 2463
ApeKI GCWGC 5 cut(s) 224, 787, 1369, 2607, 2778
ApoI RAATTY 7 cut(s) 347, 517, 747, 816, 1535, 2237, 2909
ArsI GACNNNNNNTTYG 6 cut(s) 676, 708, 710, 742, 1543, 1575
Asp700I GAANNNNTTC 2 cut(s) 1497, 2217
AspS9I GGNCC 2 cut(s) 635, 2961
AsuC2I CCSGG 1 cut(s) 3002
AsuII TTCGAA 1 cut(s) 1237
AvaI CYCGRG 4 cut(s) 40, 161, 440, 2002
AvaII GGWCC 2 cut(s) 635, 2961
AxyI CCTNAGG 2 cut(s) 1907, 2810
BalI TGGCCA 2 cut(s) 1967, 2465
BamHI GGATCC 2 cut(s) 316, 2229
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 4 cut(s) 673, 1012, 2190, 2492
BbsI GAAGAC 2 cut(s) 63, 199
Bbv12I GWGCWC 3 cut(s) 673, 2190, 2492
BbvCI CCTCAGC 1 cut(s) 77
BbvI GCAGC 5 cut(s) 211, 774, 1356, 2619, 2765
BccI CCATC 5 cut(s) 1054, 1244, 2474, 2653, 2844
BciT130I CCWGG 4 cut(s) 1110, 1524, 2245, 2412
BclI TGATCA 1 cut(s) 913
BcnI CCSGG 1 cut(s) 3002
BcoDI GTCTC 5 cut(s) 1046, 1328, 1340, 2876, 2915
BcuI ACTAGT 1 cut(s) 2765
BfaI CTAG 7 cut(s) 231, 666, 1058, 1064, 1418, 2720, 2766
BfmI CTRYAG 1 cut(s) 2355
BfrI CTTAAG 2 cut(s) 1684, 2956
BglII AGATCT 1 cut(s) 1954
BisI GCNGC 6 cut(s) 225, 788, 1370, 1895, 2608, 2779
BlsI GCNGC 6 cut(s) 226, 789, 1371, 1896, 2609, 2780
Bme1390I CCNGG 5 cut(s) 1110, 1524, 2245, 2412, 3002
Bme18I GGWCC 2 cut(s) 635, 2961
BmeT110I CYCGRG 4 cut(s) 40, 161, 440, 2002
BmgBI CACGTC 1 cut(s) 34
BmgT120I GGNCC 2 cut(s) 635, 2961
BmiI GGNNCC 4 cut(s) 239, 318, 636, 2231
BmrFI CCNGG 5 cut(s) 1110, 1524, 2245, 2412, 3002
BmrI ACTGGG 1 cut(s) 469
BmuI ACTGGG 1 cut(s) 469
BpiI GAAGAC 2 cut(s) 63, 199
BplI GAGNNNNNCTC 4 cut(s) 931, 963, 1058, 1090
BpmI CTGGAG 4 cut(s) 275, 1545, 2102, 2227
Bpu10I CCTNAGC 1 cut(s) 77
Bpu14I TTCGAA 1 cut(s) 1237
BpuEI CTTGAG 4 cut(s) 2155, 2342, 2614, 2952
BpuMI CCSGG 1 cut(s) 3002
BsaAI YACGTR 4 cut(s) 65, 1439, 2261, 2566
BsaBI GATNNNNATC 1 cut(s) 197
BsaI GGTCTC 1 cut(s) 1046
BsaJI CCNNGG 5 cut(s) 439, 477, 700, 1229, 2222
Bsc4I CCNNNNNNNGG 3 cut(s) 1109, 1662, 2870
Bse1I ACTGG 4 cut(s) 177, 464, 1588, 2455
Bse21I CCTNAGG 2 cut(s) 1907, 2810
Bse3DI GCAATG 1 cut(s) 660
Bse8I GATNNNNATC 1 cut(s) 197
BseBI CCWGG 4 cut(s) 1110, 1524, 2245, 2412
BseDI CCNNGG 5 cut(s) 439, 477, 700, 1229, 2222
BseJI GATNNNNATC 1 cut(s) 197
BseLI CCNNNNNNNGG 3 cut(s) 1109, 1662, 2870
BseMI GCAATG 1 cut(s) 660
BseNI ACTGG 4 cut(s) 177, 464, 1588, 2455
BseRI GAGGAG 1 cut(s) 1646
BseXI GCAGC 5 cut(s) 211, 774, 1356, 2619, 2765
BshFI GGCC 2 cut(s) 1967, 2465
BshNI GGYRCC 1 cut(s) 237
BsiHKAI GWGCWC 3 cut(s) 673, 2190, 2492
BsiHKCI CYCGRG 4 cut(s) 40, 161, 440, 2002
BsiSI CCGG 1 cut(s) 3001
BsiWI CGTACG 1 cut(s) 61
BslFI GGGAC 5 cut(s) 648, 1214, 1414, 2066, 2828
BslI CCNNNNNNNGG 3 cut(s) 1109, 1662, 2870
BsmAI GTCTC 5 cut(s) 1046, 1328, 1340, 2876, 2915
BsmBI CGTCTC 2 cut(s) 1328, 2915
BsmFI GGGAC 5 cut(s) 648, 1214, 1414, 2066, 2828
BsmI GAATGC 2 cut(s) 1857, 2276
BsnI GGCC 2 cut(s) 1967, 2465
Bso31I GGTCTC 1 cut(s) 1046
BsoBI CYCGRG 4 cut(s) 40, 161, 440, 2002
Bsp119I TTCGAA 1 cut(s) 1237
Bsp1286I GDGCHC 4 cut(s) 673, 1012, 2190, 2492
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 7 cut(s) 51, 409, 1349, 1895, 2625, 2947, 2964
BspANI GGCC 2 cut(s) 1967, 2465
BspLI GGNNCC 4 cut(s) 239, 318, 636, 2231
BspPI GGATC 8 cut(s) 152, 311, 324, 1936, 2224, 2237, 2840, 2992
BspT104I TTCGAA 1 cut(s) 1237
BspT107I GGYRCC 1 cut(s) 237
BspTI CTTAAG 2 cut(s) 1684, 2956
BspTNI GGTCTC 1 cut(s) 1046
BsrBI CCGCTC 1 cut(s) 2966
BsrDI GCAATG 1 cut(s) 660
BsrI ACTGG 4 cut(s) 177, 464, 1588, 2455
BssECI CCNNGG 5 cut(s) 439, 477, 700, 1229, 2222
BssT1I CCWWGG 4 cut(s) 477, 700, 1229, 2222
Bst2UI CCWGG 4 cut(s) 1110, 1524, 2245, 2412
Bst4CI ACNGT 7 cut(s) 311, 1456, 1509, 2049, 2148, 2633, 2819
Bst6I CTCTTC 3 cut(s) 270, 1343, 2147
BstAFI CTTAAG 2 cut(s) 1684, 2956
BstBAI YACGTR 4 cut(s) 65, 1439, 2261, 2566
BstBI TTCGAA 1 cut(s) 1237
BstC8I GCNNGC 3 cut(s) 1125, 1865, 2524
BstDSI CCRYGG 1 cut(s) 477
BstENI CCTNNNNNAGG 1 cut(s) 1660
BstMAI GTCTC 5 cut(s) 1046, 1328, 1340, 2876, 2915
BstMWI GCNNNNNNNGC 1 cut(s) 793
BstNI CCWGG 4 cut(s) 1110, 1524, 2245, 2412
BstNSI RCATGY 3 cut(s) 1335, 1474, 1905
BstSCI CCNGG 5 cut(s) 1108, 1522, 2243, 2410, 3000
BstSFI CTRYAG 1 cut(s) 2355
BstSNI TACGTA 3 cut(s) 1439, 2261, 2566
BstV1I GCAGC 5 cut(s) 211, 774, 1356, 2619, 2765
BstV2I GAAGAC 2 cut(s) 63, 199
BstX2I RGATCY 4 cut(s) 316, 1954, 2229, 2997
BstXI CCANNNNNNTGG 1 cut(s) 2450
BstYI RGATCY 4 cut(s) 316, 1954, 2229, 2997
Bsu36I CCTNAGG 2 cut(s) 1907, 2810
BsuRI GGCC 2 cut(s) 1967, 2465
BtgI CCRYGG 1 cut(s) 477
BtgZI GCGATG 1 cut(s) 1312
BtrI CACGTC 1 cut(s) 34
BtsIMutI CAGTG 2 cut(s) 1452, 1581
Cac8I GCNNGC 3 cut(s) 1125, 1865, 2524
CaiI CAGNNNCTG 1 cut(s) 2817
Cfr13I GGNCC 2 cut(s) 635, 2961
CseI GACGC 1 cut(s) 2515
Csp6I GTAC 6 cut(s) 28, 62, 1568, 1587, 1844, 2801
CspCI CAANNNNNGTGG 2 cut(s) 1957, 1992
CviAII CATG 7 cut(s) 326, 478, 1013, 1332, 1471, 1826, 1902
CviQI GTAC 6 cut(s) 28, 62, 1568, 1587, 1844, 2801
EaeI YGGCCR 2 cut(s) 1965, 2463
Eam1104I CTCTTC 3 cut(s) 270, 1343, 2147
EarI CTCTTC 3 cut(s) 270, 1343, 2147
EciI GGCGGA 1 cut(s) 424
Ecl136II GAGCTC 3 cut(s) 671, 2188, 2490
Eco105I TACGTA 3 cut(s) 1439, 2261, 2566
Eco130I CCWWGG 4 cut(s) 477, 700, 1229, 2222
Eco24I GRGCYC 4 cut(s) 673, 1012, 2190, 2492
Eco31I GGTCTC 1 cut(s) 1046
Eco32I GATATC 5 cut(s) 136, 843, 1835, 2167, 2575
Eco47I GGWCC 2 cut(s) 635, 2961
Eco53kI GAGCTC 3 cut(s) 671, 2188, 2490
Eco57I CTGAAG 4 cut(s) 31, 110, 1152, 2978
Eco81I CCTNAGG 2 cut(s) 1907, 2810
Eco88I CYCGRG 4 cut(s) 40, 161, 440, 2002
EcoICRI GAGCTC 3 cut(s) 671, 2188, 2490
EcoNI CCTNNNNNAGG 1 cut(s) 1660
EcoO109I RGGNCCY 1 cut(s) 635
EcoRI GAATTC 2 cut(s) 347, 2909
EcoRII CCWGG 4 cut(s) 1108, 1522, 2243, 2410
EcoRV GATATC 5 cut(s) 136, 843, 1835, 2167, 2575
EcoT14I CCWWGG 4 cut(s) 477, 700, 1229, 2222
EcoT38I GRGCYC 4 cut(s) 673, 1012, 2190, 2492
ErhI CCWWGG 4 cut(s) 477, 700, 1229, 2222
Esp3I CGTCTC 2 cut(s) 1328, 2915
FaeI CATG 7 cut(s) 329, 481, 1016, 1335, 1474, 1829, 1905
FalI AAGNNNNNCTT 4 cut(s) 1640, 1672, 2355, 2387
FaqI GGGAC 5 cut(s) 648, 1214, 1414, 2066, 2828
FatI CATG 7 cut(s) 325, 477, 1012, 1331, 1470, 1825, 1901
FauNDI CATATG 1 cut(s) 879
FbaI TGATCA 1 cut(s) 913
Fnu4HI GCNGC 6 cut(s) 225, 788, 1370, 1895, 2608, 2779
FriOI GRGCYC 4 cut(s) 673, 1012, 2190, 2492
Fsp4HI GCNGC 6 cut(s) 225, 788, 1370, 1895, 2608, 2779
FspBI CTAG 7 cut(s) 231, 666, 1058, 1064, 1418, 2720, 2766
GluI GCNGC 6 cut(s) 225, 788, 1370, 1895, 2608, 2779
GsuI CTGGAG 4 cut(s) 275, 1545, 2102, 2227
HaeIII GGCC 2 cut(s) 1967, 2465
HapII CCGG 1 cut(s) 3001
HgaI GACGC 1 cut(s) 2515
Hin1II CATG 7 cut(s) 329, 481, 1016, 1335, 1474, 1829, 1905
HinfI GANTC 9 cut(s) 185, 684, 972, 1547, 2035, 2197, 2543, 2621, 2793
HpaII CCGG 1 cut(s) 3001
Hpy166II GTNNAC 6 cut(s) 608, 769, 944, 2258, 2603, 2801
Hpy8I GTNNAC 6 cut(s) 608, 769, 944, 2258, 2603, 2801
Hpy99I CGWCG 4 cut(s) 35, 206, 1555, 2531
HpyAV CCTTC 9 cut(s) 85, 557, 648, 1666, 1736, 1824, 2031, 2653, 3002
HpyCH4III ACNGT 7 cut(s) 311, 1456, 1509, 2049, 2148, 2633, 2819
HpyF10VI GCNNNNNNNGC 1 cut(s) 793
Hsp92II CATG 7 cut(s) 329, 481, 1016, 1335, 1474, 1829, 1905
Ksp22I TGATCA 1 cut(s) 913
LmnI GCTCC 4 cut(s) 94, 966, 2014, 2083
Lsp1109I GCAGC 5 cut(s) 211, 774, 1356, 2619, 2765
MaeI CTAG 7 cut(s) 231, 666, 1058, 1064, 1418, 2720, 2766
MaeIII GTNAC 9 cut(s) 67, 1358, 1385, 1396, 1448, 1473, 2174, 2746, 3018
MbiI CCGCTC 1 cut(s) 2966
MfeI CAATTG 1 cut(s) 19
MflI RGATCY 4 cut(s) 316, 1954, 2229, 2997
MhlI GDGCHC 4 cut(s) 673, 1012, 2190, 2492
MlsI TGGCCA 2 cut(s) 1967, 2465
MluNI TGGCCA 2 cut(s) 1967, 2465
MlyI GAGTC 1 cut(s) 2802
MmeI TCCRAC 2 cut(s) 480, 2117
Mox20I TGGCCA 2 cut(s) 1967, 2465
MroXI GAANNNNTTC 2 cut(s) 1497, 2217
MscI TGGCCA 2 cut(s) 1967, 2465
MslI CAYNNNNRTG 2 cut(s) 2841, 2895
Msp20I TGGCCA 2 cut(s) 1967, 2465
MspA1I CMGCKG 2 cut(s) 51, 2627
MspCI CTTAAG 2 cut(s) 1684, 2956
MspI CCGG 1 cut(s) 3001
MspR9I CCNGG 5 cut(s) 1110, 1524, 2245, 2412, 3002
MunI CAATTG 1 cut(s) 19
Mva1269I GAATGC 2 cut(s) 1857, 2276
MvaI CCWGG 4 cut(s) 1110, 1524, 2245, 2412
MwoI GCNNNNNNNGC 1 cut(s) 793
NciI CCSGG 1 cut(s) 3002
NcoI CCATGG 1 cut(s) 477
NdeI CATATG 1 cut(s) 879
NlaIII CATG 7 cut(s) 329, 481, 1016, 1335, 1474, 1829, 1905
NlaIV GGNNCC 4 cut(s) 239, 318, 636, 2231
NmuCI GTSAC 3 cut(s) 1448, 2746, 3018
NspI RCATGY 3 cut(s) 1335, 1474, 1905
NspV TTCGAA 1 cut(s) 1237
PaeR7I CTCGAG 1 cut(s) 2002
PcsI WCGNNNNNNNCGW 1 cut(s) 688
PctI GAATGC 2 cut(s) 1857, 2276
PdmI GAANNNNTTC 2 cut(s) 1497, 2217
PfeI GAWTC 8 cut(s) 185, 684, 972, 1547, 2035, 2197, 2543, 2621
Pfl23II CGTACG 1 cut(s) 61
PflMI CCANNNNNTGG 1 cut(s) 1109
PfoI TCCNGGA 2 cut(s) 2410, 3000
PkrI GCNGC 6 cut(s) 226, 789, 1371, 1896, 2609, 2780
PleI GAGTC 1 cut(s) 2801
PpsI GAGTC 1 cut(s) 2801
Ppu21I YACGTR 4 cut(s) 65, 1439, 2261, 2566
PpuMI RGGWCCY 1 cut(s) 635
Psp124BI GAGCTC 3 cut(s) 673, 2190, 2492
Psp1406I AACGTT 3 cut(s) 1144, 2254, 2712
Psp5II RGGWCCY 1 cut(s) 635
Psp6I CCWGG 4 cut(s) 1108, 1522, 2243, 2410
PspGI CCWGG 4 cut(s) 1108, 1522, 2243, 2410
PspLI CGTACG 1 cut(s) 61
PspN4I GGNNCC 4 cut(s) 239, 318, 636, 2231
PspPI GGNCC 2 cut(s) 635, 2961
PspPPI RGGWCCY 1 cut(s) 635
PspXI VCTCGAGB 1 cut(s) 2002
PsrI GAACNNNNNNTAC 2 cut(s) 20, 52
PstNI CAGNNNCTG 1 cut(s) 2817
PsuI RGATCY 4 cut(s) 316, 1954, 2229, 2997
RsaI GTAC 6 cut(s) 29, 63, 1569, 1588, 1845, 2802
RsaNI GTAC 6 cut(s) 28, 62, 1568, 1587, 1844, 2801
RseI CAYNNNNRTG 2 cut(s) 2841, 2895
SacI GAGCTC 3 cut(s) 673, 2190, 2492
SatI GCNGC 6 cut(s) 225, 788, 1370, 1895, 2608, 2779
Sau96I GGNCC 2 cut(s) 635, 2961
SchI GAGTC 1 cut(s) 2802
ScrFI CCNGG 5 cut(s) 1110, 1524, 2245, 2412, 3002
SduI GDGCHC 4 cut(s) 673, 1012, 2190, 2492
SfcI CTRYAG 1 cut(s) 2355
Sfr274I CTCGAG 1 cut(s) 2002
SfuI TTCGAA 1 cut(s) 1237
SinI GGWCC 2 cut(s) 635, 2961
SlaI CTCGAG 1 cut(s) 2002
SmiMI CAYNNNNRTG 2 cut(s) 2841, 2895
SmlI CTYRAG 7 cut(s) 1684, 2002, 2170, 2321, 2593, 2956, 2967
SmoI CTYRAG 7 cut(s) 1684, 2002, 2170, 2321, 2593, 2956, 2967
SnaBI TACGTA 3 cut(s) 1439, 2261, 2566
SpeI ACTAGT 1 cut(s) 2765
SsiI CCGC 7 cut(s) 51, 409, 1349, 1895, 2625, 2947, 2964
SspI AATATT 2 cut(s) 1269, 2854
SspMI CTAG 7 cut(s) 231, 666, 1058, 1064, 1418, 2720, 2766
SstI GAGCTC 3 cut(s) 673, 2190, 2492
StyD4I CCNGG 5 cut(s) 1108, 1522, 2243, 2410, 3000
StyI CCWWGG 4 cut(s) 477, 700, 1229, 2222
TaaI ACNGT 7 cut(s) 311, 1456, 1509, 2049, 2148, 2633, 2819
TatI WGTACW 2 cut(s) 1567, 1843
TauI GCSGC 1 cut(s) 1897
TfiI GAWTC 8 cut(s) 185, 684, 972, 1547, 2035, 2197, 2543, 2621
TscAI CASTG 2 cut(s) 1459, 1588
TseFI GTSAC 3 cut(s) 1448, 2746, 3018
TseI GCWGC 5 cut(s) 224, 787, 1369, 2607, 2778
Tsp45I GTSAC 3 cut(s) 1448, 2746, 3018
TspDTI ATGAA 7 cut(s) 437, 483, 896, 1814, 2078, 2747, 2811
TspGWI ACGGA 1 cut(s) 1568
TspRI CASTG 2 cut(s) 1459, 1588
Van91I CCANNNNNTGG 1 cut(s) 1109
Vha464I CTTAAG 2 cut(s) 1684, 2956
VpaK11BI GGWCC 2 cut(s) 635, 2961
XagI CCTNNNNNAGG 1 cut(s) 1660
XapI RAATTY 7 cut(s) 347, 517, 747, 816, 1535, 2237, 2909
XbaI TCTAGA 1 cut(s) 1063
XceI RCATGY 3 cut(s) 1335, 1474, 1905
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
XhoI CTCGAG 1 cut(s) 2002
XmnI GAANNNNTTC 2 cut(s) 1497, 2217
XspI CTAG 7 cut(s) 231, 666, 1058, 1064, 1418, 2720, 2766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.