AT5G46270

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002912
Physical Location & Seq
Reverse (-)
18763972 .. 18769090
5119 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46270.5

Sequence Viewer

Length: 3420 bp
ATGTGTAGTAATTTTCCTCCTCTCATTCTCTCTATGGCTTCTTCATCTTCTTCTCGAAACTGGTTATATGATGTTTTCTTGAGCTTCAGAGGGGGAGACGTCCGCGTAACATTCCGTAGCCACTTTCTCAAAGAGCTTGATCGGAAACTGATCACTGCATTCAGAGACAATGAGATCGAGAGAAGCCACTCTCTCTGGCCCGATCTTGAACAAGCCATCAAGGATTCCAGAATCGCAGTGGTCATTTTCTCCAAAAACTACGCTTCATCGAGCTGGTGTCTTAACGAGTTGCTGGAGATTGTGAATTGCAATGATAAAATTGTCATACCAGTTTTCTACGGAGTGGATCCTTCCCAAGTGAGGCATCAAATCGGTGACTTTGGAAAGATCTTTGAAAAGACTTGCAAGAGACAAACAGAGCAAGTGAAAAACCAATGGAAGAAAGCGTTGACTGATGTAGCTAATATGCTTGGATTTGATTCTGCGACATGGGATGACGAAGCAAAAATGATTGAAGAAATAGCCAATGATGTTTTGGCTAAACTGCTTTTAACTACACCCAAGGACTTTGAAAATTTTGTTGGCATCGAAGATCATATTGCAAATATGAGTGTATTGTTGAAATTGGAAGCTGAGGAAGTGAGAATGGTTGGTATATGGGGTTCCTCGGGGATTGGTAAGACTACCATTGCAAGAGCTCTGTTTAACCAACTTTCTCGGCATTTCCCAGTTAGCAAATTCATAGACAGAGCTTTTGTATATAAGAGTAGAGAAATTTTTAGTAGAGCCAATCCGGACGACCACAACATGAAGTTACATTTACAAGAGAAACTCCTCTCTGAAATTCTGAGGATGCCAGACATAAAGATCGACCACCTAGGTGTACTAGGAGAGAGGCTACAACACCAAAAAGTTCTTATTATCGTTGATGATTTGGATGACCAAGTGATCTTAGATTCATTGGTGGGTCAAACTCAATGGTTTGGAAGTGGGAGCAGAATCATTGCGGTTACAAATAATAAGCATTTCTTAAGGGCTCATGAGATTGATCATATTTATGAAGTTAGTCTCCCAACTCAGCAACACGCTCTTGCAATGTTATGTCAATCTGCTTTTAGGAAAAAATCTCCACCAGAAGGTTTTGAGATGCTTGTAGTTCAAGTTGCAAGACATGTGGATAGTCTTCCTTTGGGTCTTAATGTTTTGGGTTCATATTTGAGGGGCCGGGATAAGGAGTACTGGATGGAAATGCTGCCAAGGCTTGAGAATGGTCTACACGATAAAATTGAGAAAATACTAAGAATCAGCTATGATGGTTTAGGTAGCGAAGAGGATAAAGCGATATTTCGTCATATCGCATGTCTTTTCAATCATATGGAAGTCACAACCATCACGTCGTTGCTCACAGACTTGGGTATTAATATTGGGCTAAAAAACCTAGTGGATAAGTCCATCATTCATGTCAGACGGGGTTGTGTGGAGATGCACCGTATGCTACAAGAAATGGGCAGAAAAATTGTTCGCACACAGTCCATTGACAAGCCTGGGAAACGAGAATTTCTGGTGGATCCAAATGATATTTCTGATGTACTCAGTGAAGGCATTGGTACTCAAAAGGTTTTAGGTATATCTTTAAATACAGGTGAAATTGATGAGTTGTATGTACATGAGAGTGCTTTCAAAGGGATGAGTAATCTCCGTTTCTTAGAAATTGATTCGAAGAACTTTGGAAAAGCAGGTAGATTGTACTTACCCGAAAGTTTGGACTATTTACCCCCTAGACTCAAACTATTGTGTTGGCCCAATTTTCCTATGAGATGTATGCCTTCTAATTTTCGTCCTGAAAACCTTGTGACGCTCAAAATGCCGAATAGCAAGCTACATAAGCTGTGGGAAGGAGTTGCGTCGCTTACATGCCTAAAGGAAATGGATATGGTTGGATCATCAAACCTGAAAGAAATTCCAGATCTTTCCATGCCTACTAATCTTGAGATACTTAAACTTGGTTTTTGTAAGAGTTTGGTGGAGCTTCCTTCCTCTATAAGAAATCTCAATAAACTATTGAAATTGGACATGGAATTCTGCCATAGTCTGGAAATTCTCCCAACCGGCTTCAACCTCAAATCTCTCGACCACCTCAACTTCCGATATTGCTCGGAGTTGAGGACTTTTCCCGAATTCTCAACCAACATCTCCGTTCTCATGCTTTTTGGAACAAACATTGAAGAATTCCCTAACCTCGAGAATCTTGTTGAGCTTAGCTTATCAAAAGAAGAGAGTGATGGGAAACAATGGGATGGAGTGAAGCCGCTTACGCCCTTCTTGGAGATGTTGTCTCCCACTTTGAAGAGCTTGAAGCTCGAGAATATTCCAAGTTTGGTGGAGCTTCCTTCCTCATTTCAGAATCTCAATCAACTGAAGGAGTTGAGCATCACATACTGCAGAAATCTGGAGACTCTTCCCACAGGAATCAACCTCAAATCTCTCAATTACCTTTGTTTCAAAGGATGCTCACAGTTGAGGAGCTTTCCTGAAATCTCAACTAACATCTCAGTTCTCAATCTAGAAGAAACAGGGATTGAAGAGGTTCCTTGGCAGATCGAGAACTTCTTTAACCTCACTAAGCTAACTATGAGGAGTTGCAGCAAGCTAAAATGTTTATCCCTAAACATTCCTAAAATGAAAACTCTGTGGGACGTTGACTTTTCAGATTGTGCGGCGTTGACTGTAGTTAACTTAAGTGGCTATCCAAGTGACACACTTTCCGAGGAGGAGGATGATAGTTTGGACCCCTTTCTCGATTTCAGGGGCTGCTTCAGCTTGGATCCAGAAACTGTCCTTCACCAAGAGTCAGTTATTTTCAACTCCATGGCATTTCCAGGTGAACAAGTGCCATCATATTTCACGTACCGTACTACTGGAACCTCCACCATTCTCCCCAATATCCCTCTACTTCCAACACAACTCTCTCAACCATTCTTCAGATTTAGGGTTTGTGCGGTGGCAACCGCTTTCAATATAGTATCCATCCAGGTAAATAGTCGGTTCACAGGCAGATTTGGGAACAGCTTTGATTTCTTTGGTGAGGGACATGAACTCATGGAAATACGGAAGGGTGATATGTTGATATTTGACTGTCGTATCCCTTTCAACGAAGATAATGCTCTTCTAGCTCGAGGGGACTACGATCACGTGGATATGAATATTCATATAAGTGGAGATTCAGAATCTACGTTCGAATTAAAAGGATGGGGTATACGCCTCTTAGAGGACTGTTCATCAGTGGAGAACCGACTTGTTAATCCAACACATGCGTATACACCTGTTCAAGGACTTGTTAATGAGATTGAACACAATGAAGACTCTGGAGATAACAATGTAGAGACTAAGAGAAGCGCGAAGCGAATGCGGATCACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1139

Amino Acids

129.68

Weight (kDa)

5.6

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 196 1.7e-55 TIR domain
TIR_2 PF13676 25 - 118 1.2e-10 TIR domain
AAA_16 PF13191 193 - 314 7.5e-07 AAA ATPase domain
NB-ARC PF00931 208 - 375 9.1e-11 NB-ARC domain
WHD_ROQ1 PF23282 443 - 511 4.1e-24 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 616 - 634 1e-08 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 102
Acc36I ACCTGC 1 cut(s) 1727
AccB7I CCANNNNNTGG 1 cut(s) 2092
AccI GTMKAC 3 cut(s) 1273, 3256, 3317
AccII CGCG 2 cut(s) 105, 3398
AccIII TCCGGA 1 cut(s) 793
AciI CCGC 7 cut(s) 103, 1007, 2309, 2717, 2999, 3009, 3409
AclWI GGATC 8 cut(s) 341, 354, 1562, 1575, 1948, 2819, 2832, 3419
AcuI CTGAAG 4 cut(s) 70, 2438, 2800, 2965
AcvI CACGTG 1 cut(s) 3193
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 8 cut(s) 885, 1238, 1590, 1609, 1665, 1748, 2909, 2914
AfiI CCNNNNNNNGG 6 cut(s) 360, 1136, 1231, 2092, 3268, 3329
AflII CTTAAG 2 cut(s) 1030, 2737
AflIII ACRYGT 1 cut(s) 1171
AjiI CACGTC 1 cut(s) 1395
AjnI CCWGG 3 cut(s) 1543, 2878, 3030
AjuI GAANNNNNNNTTGG 4 cut(s) 564, 596, 1564, 1596
AleI CACNNNNGTG 1 cut(s) 879
Alw21I GWGCWC 1 cut(s) 700
Alw26I GTCTC 7 cut(s) 90, 159, 403, 1073, 2340, 2447, 3377
AlwI GGATC 8 cut(s) 341, 354, 1562, 1575, 1948, 2819, 2832, 3419
AlwNI CAGNNNCTG 2 cut(s) 2811, 2834
Ama87I CYCGRG 4 cut(s) 667, 2240, 2360, 3174
Aor13HI TCCGGA 1 cut(s) 793
AoxI GGCC 3 cut(s) 197, 1222, 1799
ApeKI GCWGC 3 cut(s) 1252, 2643, 2811
ArsI GACNNNNNNTTYG 2 cut(s) 737, 769
AseI ATTAAT 1 cut(s) 1419
Asp700I GAANNNNTTC 1 cut(s) 2586
AspA2I CCTAGG 1 cut(s) 877
AspLEI GCGC 1 cut(s) 3398
AspS9I GGNCC 4 cut(s) 198, 1222, 1800, 2788
AsuC2I CCSGG 1 cut(s) 1226
AsuHPI GGTGA 6 cut(s) 386, 1655, 2834, 2894, 3095, 3128
AsuII TTCGAA 2 cut(s) 1718, 3237
AvaI CYCGRG 4 cut(s) 667, 2240, 2360, 3174
AvaII GGWCC 1 cut(s) 2788
AvrII CCTAGG 1 cut(s) 877
BaeI ACNNNNGTAYC 2 cut(s) 3124, 3157
BamHI GGATCC 3 cut(s) 346, 1567, 2824
BanII GRGCYC 2 cut(s) 700, 1039
BbrPI CACGTG 1 cut(s) 3193
BbsI GAAGAC 2 cut(s) 1175, 3366
Bbv12I GWGCWC 1 cut(s) 700
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 3 cut(s) 1239, 2655, 2798
BcgI CGANNNNNNTGC 3 cut(s) 3143, 3177, 3388
BciT130I CCWGG 3 cut(s) 1545, 2880, 3032
BciVI GTATCC 2 cut(s) 3034, 3152
BclI TGATCA 2 cut(s) 150, 1048
BcnI CCSGG 1 cut(s) 1226
BcoDI GTCTC 7 cut(s) 90, 159, 403, 1073, 2340, 2447, 3377
BfaI CTAG 6 cut(s) 878, 887, 1439, 1779, 2564, 3170
BfmI CTRYAG 2 cut(s) 2440, 2727
BfrI CTTAAG 2 cut(s) 1030, 2737
BfuAI ACCTGC 1 cut(s) 1727
BfuI GTATCC 2 cut(s) 3034, 3152
BglII AGATCT 2 cut(s) 387, 1966
BisI GCNGC 5 cut(s) 1253, 2309, 2644, 2718, 2812
BlnI CCTAGG 1 cut(s) 877
BlpI GCTNAGC 1 cut(s) 2258
BlsI GCNGC 5 cut(s) 1254, 2310, 2645, 2719, 2813
BmcAI AGTACT 1 cut(s) 1238
Bme1390I CCNGG 4 cut(s) 1226, 1545, 2880, 3032
Bme18I GGWCC 1 cut(s) 2788
BmeT110I CYCGRG 4 cut(s) 667, 2240, 2360, 3174
BmgBI CACGTC 1 cut(s) 1395
BmgT120I GGNCC 4 cut(s) 198, 1222, 1800, 2788
BmiI GGNNCC 8 cut(s) 348, 664, 1223, 1569, 2589, 2790, 2826, 2923
BmrFI CCNGG 4 cut(s) 1226, 1545, 2880, 3032
BmrI ACTGGG 1 cut(s) 722
BmsI GCATC 7 cut(s) 373, 594, 843, 1137, 1473, 2439, 2498
BmuI ACTGGG 1 cut(s) 722
BpiI GAAGAC 2 cut(s) 1175, 3366
BpmI CTGGAG 3 cut(s) 314, 2471, 3387
Bpu10I CCTNAGC 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 2258
Bpu14I TTCGAA 2 cut(s) 1718, 3237
BpuEI CTTGAG 3 cut(s) 100, 1283, 2009
BpuMI CCSGG 1 cut(s) 1226
BsaAI YACGTR 3 cut(s) 2907, 3193, 3417
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 8 cut(s) 561, 666, 877, 1256, 1544, 2591, 2766, 2868
BsaWI WCCGGW 1 cut(s) 793
BsaXI ACNNNNNCTCC 2 cut(s) 2318, 2348
Bsc4I CCNNNNNNNGG 6 cut(s) 360, 1136, 1231, 2092, 3268, 3329
Bse118I RCCGGY 1 cut(s) 2108
Bse1I ACTGG 5 cut(s) 65, 329, 728, 1244, 2923
Bse3DI GCAATG 4 cut(s) 316, 687, 1002, 1101
BseAI TCCGGA 1 cut(s) 793
BseBI CCWGG 3 cut(s) 1545, 2880, 3032
BseDI CCNNGG 8 cut(s) 561, 666, 877, 1256, 1544, 2591, 2766, 2868
BseLI CCNNNNNNNGG 6 cut(s) 360, 1136, 1231, 2092, 3268, 3329
BseMI GCAATG 4 cut(s) 316, 687, 1002, 1101
BseMII CTCAG 5 cut(s) 624, 839, 1091, 1606, 2565
BseNI ACTGG 5 cut(s) 65, 329, 728, 1244, 2923
BseRI GAGGAG 6 cut(s) 9, 824, 2536, 2650, 2783, 2786
BseXI GCAGC 3 cut(s) 1239, 2655, 2798
Bsh1236I CGCG 2 cut(s) 105, 3398
BshFI GGCC 3 cut(s) 199, 1224, 1801
BsiHKAI GWGCWC 1 cut(s) 700
BsiHKCI CYCGRG 4 cut(s) 667, 2240, 2360, 3174
BsiSI CCGG 3 cut(s) 794, 1225, 2109
BslFI GGGAC 3 cut(s) 2708, 3102, 3194
BslI CCNNNNNNNGG 6 cut(s) 360, 1136, 1231, 2092, 3268, 3329
BsmAI GTCTC 7 cut(s) 90, 159, 403, 1073, 2340, 2447, 3377
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 3 cut(s) 2708, 3102, 3194
BsmI GAATGC 2 cut(s) 158, 3411
BsnI GGCC 3 cut(s) 199, 1224, 1801
BsoBI CYCGRG 4 cut(s) 667, 2240, 2360, 3174
Bsp119I TTCGAA 2 cut(s) 1718, 3237
Bsp1286I GDGCHC 2 cut(s) 700, 1039
Bsp13I TCCGGA 1 cut(s) 793
Bsp1407I TGTACA 1 cut(s) 1663
Bsp1720I GCTNAGC 1 cut(s) 2258
Bsp19I CCATGG 1 cut(s) 2868
BspACI CCGC 7 cut(s) 103, 1007, 2309, 2717, 2999, 3009, 3409
BspANI GGCC 3 cut(s) 199, 1224, 1801
BspCNI CTCAG 5 cut(s) 625, 840, 1090, 1605, 2564
BspEI TCCGGA 1 cut(s) 793
BspFNI CGCG 2 cut(s) 105, 3398
BspHI TCATGA 1 cut(s) 1039
BspLI GGNNCC 8 cut(s) 348, 664, 1223, 1569, 2589, 2790, 2826, 2923
BspMAI CTGCAG 1 cut(s) 2444
BspMI ACCTGC 1 cut(s) 1727
BspPI GGATC 8 cut(s) 341, 354, 1562, 1575, 1948, 2819, 2832, 3419
BspQI GCTCTTC 2 cut(s) 2342, 3171
BspT104I TTCGAA 2 cut(s) 1718, 3237
BspTI CTTAAG 2 cut(s) 1030, 2737
BsrDI GCAATG 4 cut(s) 316, 687, 1002, 1101
BsrFI RCCGGY 1 cut(s) 2108
BsrGI TGTACA 1 cut(s) 1663
BsrI ACTGG 5 cut(s) 65, 329, 728, 1244, 2923
BssAI RCCGGY 1 cut(s) 2108
BssECI CCNNGG 8 cut(s) 561, 666, 877, 1256, 1544, 2591, 2766, 2868
BssNAI GTATAC 2 cut(s) 3257, 3318
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 5 cut(s) 561, 877, 1256, 2591, 2868
Bst1107I GTATAC 2 cut(s) 3257, 3318
Bst2UI CCWGG 3 cut(s) 1545, 2880, 3032
Bst4CI ACNGT 8 cut(s) 1490, 1530, 2517, 2728, 2836, 2912, 3137, 3275
Bst6I CTCTTC 6 cut(s) 1323, 2268, 2342, 2463, 2577, 3171
BstACI GRCGYC 1 cut(s) 99
BstAFI CTTAAG 2 cut(s) 1030, 2737
BstAPI GCANNNNNTGC 1 cut(s) 1492
BstAUI TGTACA 1 cut(s) 1663
BstBAI YACGTR 3 cut(s) 2907, 3193, 3417
BstBI TTCGAA 2 cut(s) 1718, 3237
BstC8I GCNNGC 2 cut(s) 1877, 2648
BstDSI CCRYGG 1 cut(s) 2868
BstENI CCTNNNNNAGG 2 cut(s) 3266, 3327
BstFNI CGCG 2 cut(s) 105, 3398
BstHHI GCGC 1 cut(s) 3398
BstMAI GTCTC 7 cut(s) 90, 159, 403, 1073, 2340, 2447, 3377
BstMWI GCNNNNNNNGC 7 cut(s) 1258, 1492, 1864, 1885, 2314, 2817, 3170
BstNI CCWGG 3 cut(s) 1545, 2880, 3032
BstNSI RCATGY 4 cut(s) 1175, 1362, 1917, 3314
BstSCI CCNGG 4 cut(s) 1224, 1543, 2878, 3030
BstSFI CTRYAG 2 cut(s) 2440, 2727
BstUI CGCG 2 cut(s) 105, 3398
BstV1I GCAGC 3 cut(s) 1239, 2655, 2798
BstV2I GAAGAC 2 cut(s) 1175, 3366
BstX2I RGATCY 5 cut(s) 346, 387, 1567, 1966, 2824
BstYI RGATCY 5 cut(s) 346, 387, 1567, 1966, 2824
BstZ17I GTATAC 2 cut(s) 3257, 3318
BsuI GTATCC 2 cut(s) 3034, 3152
BsuRI GGCC 3 cut(s) 199, 1224, 1801
BtgI CCRYGG 1 cut(s) 2868
BtrI CACGTC 1 cut(s) 1395
BtsI GCAGTG 2 cut(s) 153, 243
BtsIMutI CAGTG 4 cut(s) 153, 243, 1600, 3288
BveI ACCTGC 1 cut(s) 1727
Cac8I GCNNGC 2 cut(s) 1877, 2648
CaiI CAGNNNCTG 2 cut(s) 2811, 2834
CciI TCATGA 1 cut(s) 1039
CfoI GCGC 1 cut(s) 3398
Cfr10I RCCGGY 1 cut(s) 2108
Cfr13I GGNCC 4 cut(s) 198, 1222, 1800, 2788
CseI GACGC 2 cut(s) 1864, 1893
Csp6I GTAC 8 cut(s) 884, 1237, 1589, 1608, 1664, 1747, 2908, 2913
CspCI CAANNNNNGTGG 4 cut(s) 1155, 1190, 2361, 2396
CviQI GTAC 8 cut(s) 884, 1237, 1589, 1608, 1664, 1747, 2908, 2913
DraI TTTAAA 1 cut(s) 1635
Eam1104I CTCTTC 6 cut(s) 1323, 2268, 2342, 2463, 2577, 3171
EarI CTCTTC 6 cut(s) 1323, 2268, 2342, 2463, 2577, 3171
Ecl136II GAGCTC 1 cut(s) 698
Eco130I CCWWGG 5 cut(s) 561, 877, 1256, 2591, 2868
Eco24I GRGCYC 2 cut(s) 700, 1039
Eco47I GGWCC 1 cut(s) 2788
Eco53kI GAGCTC 1 cut(s) 698
Eco57I CTGAAG 4 cut(s) 70, 2438, 2800, 2965
Eco72I CACGTG 1 cut(s) 3193
Eco88I CYCGRG 4 cut(s) 667, 2240, 2360, 3174
EcoICRI GAGCTC 1 cut(s) 698
EcoNI CCTNNNNNAGG 2 cut(s) 3266, 3327
EcoRI GAATTC 3 cut(s) 2078, 2177, 2228
EcoRII CCWGG 3 cut(s) 1543, 2878, 3030
EcoT14I CCWWGG 5 cut(s) 561, 877, 1256, 2591, 2868
EcoT38I GRGCYC 2 cut(s) 700, 1039
ErhI CCWWGG 5 cut(s) 561, 877, 1256, 2591, 2868
Esp3I CGTCTC 1 cut(s) 90
FalI AAGNNNNNCTT 2 cut(s) 1013, 1045
FaqI GGGAC 3 cut(s) 2708, 3102, 3194
FauNDI CATATG 1 cut(s) 1374
FbaI TGATCA 2 cut(s) 150, 1048
FblI GTMKAC 3 cut(s) 1273, 3256, 3317
Fnu4HI GCNGC 5 cut(s) 1253, 2309, 2644, 2718, 2812
FriOI GRGCYC 2 cut(s) 700, 1039
Fsp4HI GCNGC 5 cut(s) 1253, 2309, 2644, 2718, 2812
FspBI CTAG 6 cut(s) 878, 887, 1439, 1779, 2564, 3170
GlaI GCGC 1 cut(s) 3397
GluI GCNGC 5 cut(s) 1253, 2309, 2644, 2718, 2812
GsuI CTGGAG 3 cut(s) 314, 2471, 3387
HaeIII GGCC 3 cut(s) 199, 1224, 1801
HapII CCGG 3 cut(s) 794, 1225, 2109
HgaI GACGC 2 cut(s) 1864, 1893
HhaI GCGC 1 cut(s) 3398
Hin1I GRCGYC 1 cut(s) 99
Hin6I GCGC 1 cut(s) 3396
HinP1I GCGC 1 cut(s) 3396
HincII GTYRAC 4 cut(s) 450, 2701, 2724, 2734
HindII GTYRAC 4 cut(s) 450, 2701, 2724, 2734
HpaI GTTAAC 1 cut(s) 2734
HpaII CCGG 3 cut(s) 794, 1225, 2109
HphI GGTGA 6 cut(s) 386, 1655, 2834, 2894, 3095, 3128
Hpy99I CGWCG 2 cut(s) 1399, 1909
HpyCH4III ACNGT 8 cut(s) 1490, 1530, 2517, 2728, 2836, 2912, 3137, 3275
HpyCH4IV ACGT 7 cut(s) 99, 1394, 2697, 2906, 3192, 3233, 3416
HpyF10VI GCNNNNNNNGC 7 cut(s) 1258, 1492, 1864, 1885, 2314, 2817, 3170
HpySE526I ACGT 7 cut(s) 99, 1394, 2697, 2906, 3192, 3233, 3416
Hsp92I GRCGYC 1 cut(s) 99
HspAI GCGC 1 cut(s) 3396
Kpn2I TCCGGA 1 cut(s) 793
Ksp22I TGATCA 2 cut(s) 150, 1048
KspAI GTTAAC 1 cut(s) 2734
LguI GCTCTTC 2 cut(s) 2342, 3171
LmnI GCTCC 4 cut(s) 993, 2026, 2383, 2523
Lsp1109I GCAGC 3 cut(s) 1239, 2655, 2798
LweI GCATC 7 cut(s) 373, 594, 843, 1137, 1473, 2439, 2498
MaeI CTAG 6 cut(s) 878, 887, 1439, 1779, 2564, 3170
MaeII ACGT 7 cut(s) 99, 1394, 2697, 2906, 3192, 3233, 3416
MaeIII GTNAC 7 cut(s) 106, 374, 813, 1009, 1381, 1852, 2753
MflI RGATCY 5 cut(s) 346, 387, 1567, 1966, 2824
MhlI GDGCHC 2 cut(s) 700, 1039
MlyI GAGTC 4 cut(s) 1776, 2449, 2858, 3356
MmeI TCCRAC 3 cut(s) 1918, 2981, 3329
MroI TCCGGA 1 cut(s) 793
MroXI GAANNNNTTC 1 cut(s) 2586
MslI CAYNNNNRTG 4 cut(s) 879, 1056, 1671, 3213
MspCI CTTAAG 2 cut(s) 1030, 2737
MspI CCGG 3 cut(s) 794, 1225, 2109
MspR9I CCNGG 4 cut(s) 1226, 1545, 2880, 3032
Mva1269I GAATGC 2 cut(s) 158, 3411
MvaI CCWGG 3 cut(s) 1545, 2880, 3032
MvnI CGCG 2 cut(s) 105, 3398
MwoI GCNNNNNNNGC 7 cut(s) 1258, 1492, 1864, 1885, 2314, 2817, 3170
NciI CCSGG 1 cut(s) 1226
NcoI CCATGG 1 cut(s) 2868
NdeI CATATG 1 cut(s) 1374
NlaIV GGNNCC 8 cut(s) 348, 664, 1223, 1569, 2589, 2790, 2826, 2923
NmeAIII GCCGAG 1 cut(s) 697
NmuCI GTSAC 4 cut(s) 374, 1381, 1852, 2753
NspI RCATGY 4 cut(s) 1175, 1362, 1917, 3314
NspV TTCGAA 2 cut(s) 1718, 3237
OliI CACNNNNGTG 1 cut(s) 879
PaeR7I CTCGAG 3 cut(s) 2240, 2360, 3174
PagI TCATGA 1 cut(s) 1039
PciI ACATGT 1 cut(s) 1171
PciSI GCTCTTC 2 cut(s) 2342, 3171
PctI GAATGC 2 cut(s) 158, 3411
PdmI GAANNNNTTC 1 cut(s) 2586
PflMI CCANNNNNTGG 1 cut(s) 2092
PkrI GCNGC 5 cut(s) 1254, 2310, 2645, 2719, 2813
PleI GAGTC 4 cut(s) 1776, 2449, 2857, 3356
PmaCI CACGTG 1 cut(s) 3193
PmlI CACGTG 1 cut(s) 3193
PpsI GAGTC 4 cut(s) 1776, 2449, 2857, 3356
Ppu21I YACGTR 3 cut(s) 2907, 3193, 3417
PscI ACATGT 1 cut(s) 1171
PshBI ATTAAT 1 cut(s) 1419
Psp124BI GAGCTC 1 cut(s) 700
Psp6I CCWGG 3 cut(s) 1543, 2878, 3030
PspCI CACGTG 1 cut(s) 3193
PspGI CCWGG 3 cut(s) 1543, 2878, 3030
PspN4I GGNNCC 8 cut(s) 348, 664, 1223, 1569, 2589, 2790, 2826, 2923
PspPI GGNCC 4 cut(s) 198, 1222, 1800, 2788
PspXI VCTCGAGB 1 cut(s) 3174
PsrI GAACNNNNNNTAC 2 cut(s) 646, 678
PstI CTGCAG 1 cut(s) 2444
PstNI CAGNNNCTG 2 cut(s) 2811, 2834
PsuI RGATCY 5 cut(s) 346, 387, 1567, 1966, 2824
RsaI GTAC 8 cut(s) 885, 1238, 1590, 1609, 1665, 1748, 2909, 2914
RsaNI GTAC 8 cut(s) 884, 1237, 1589, 1608, 1664, 1747, 2908, 2913
RseI CAYNNNNRTG 4 cut(s) 879, 1056, 1671, 3213
SacI GAGCTC 1 cut(s) 700
SapI GCTCTTC 2 cut(s) 2342, 3171
SatI GCNGC 5 cut(s) 1253, 2309, 2644, 2718, 2812
Sau96I GGNCC 4 cut(s) 198, 1222, 1800, 2788
ScaI AGTACT 1 cut(s) 1238
SchI GAGTC 4 cut(s) 1776, 2449, 2858, 3356
ScrFI CCNGG 4 cut(s) 1226, 1545, 2880, 3032
SduI GDGCHC 2 cut(s) 700, 1039
SfaNI GCATC 7 cut(s) 373, 594, 843, 1137, 1473, 2439, 2498
SfcI CTRYAG 2 cut(s) 2440, 2727
Sfr274I CTCGAG 3 cut(s) 2240, 2360, 3174
SfuI TTCGAA 2 cut(s) 1718, 3237
SinI GGWCC 1 cut(s) 2788
SlaI CTCGAG 3 cut(s) 2240, 2360, 3174
SmiMI CAYNNNNRTG 4 cut(s) 879, 1056, 1671, 3213
SmlI CTYRAG 8 cut(s) 79, 1030, 1262, 1988, 2240, 2360, 2737, 3174
SmoI CTYRAG 8 cut(s) 79, 1030, 1262, 1988, 2240, 2360, 2737, 3174
SsiI CCGC 7 cut(s) 103, 1007, 2309, 2717, 2999, 3009, 3409
SspI AATATT 3 cut(s) 1423, 2368, 3205
SspMI CTAG 6 cut(s) 878, 887, 1439, 1779, 2564, 3170
SstI GAGCTC 1 cut(s) 700
StyD4I CCNGG 4 cut(s) 1224, 1543, 2878, 3030
StyI CCWWGG 5 cut(s) 561, 877, 1256, 2591, 2868
TaaI ACNGT 8 cut(s) 1490, 1530, 2517, 2728, 2836, 2912, 3137, 3275
TaiI ACGT 7 cut(s) 102, 1397, 2700, 2909, 3195, 3236, 3419
TatI WGTACW 5 cut(s) 883, 1236, 1588, 1663, 1746
TauI GCSGC 2 cut(s) 2311, 2720
TscAI CASTG 4 cut(s) 160, 243, 1600, 3288
TseFI GTSAC 4 cut(s) 374, 1381, 1852, 2753
TseI GCWGC 3 cut(s) 1252, 2643, 2811
Tsp45I GTSAC 4 cut(s) 374, 1381, 1852, 2753
TspGWI ACGGA 5 cut(s) 104, 354, 1688, 2185, 3124
TspRI CASTG 4 cut(s) 160, 243, 1600, 3288
Van91I CCANNNNNTGG 1 cut(s) 2092
Vha464I CTTAAG 2 cut(s) 1030, 2737
VpaK11BI GGWCC 1 cut(s) 2788
VspI ATTAAT 1 cut(s) 1419
XagI CCTNNNNNAGG 2 cut(s) 3266, 3327
XbaI TCTAGA 1 cut(s) 2563
XceI RCATGY 4 cut(s) 1175, 1362, 1917, 3314
XcmI CCANNNNNNNNNTGG 2 cut(s) 235, 532
XhoI CTCGAG 3 cut(s) 2240, 2360, 3174
XmaJI CCTAGG 1 cut(s) 877
XmiI GTMKAC 3 cut(s) 1273, 3256, 3317
XmnI GAANNNNTTC 1 cut(s) 2586
XspI CTAG 6 cut(s) 878, 887, 1439, 1779, 2564, 3170
ZraI GACGTC 1 cut(s) 100
ZrmI AGTACT 1 cut(s) 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.