AT5G46490

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002912
Physical Location & Seq
Forward (+)
18850645 .. 18853896
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46490.4

Sequence Viewer

Length: 1074 bp
ATGGCTTCTTCATCTTCTTCTCGAAATTGGTTATATGATGTTTTCTTGAGCTTCAGAGGGGGAGACGTCCGTGTAACATTCCGTAGCCACTTTCTCAAGGAGTTCGATCGGAAACTGATCACTGCTTTCAGAGACAATGAGATCGAGAGAAGCCACTCTCTTTGGCCCGATCTTGAACAAGCGATCAAGGACTCCAGAATCGCAGTGGTCGTTTTCTCCAAAAACTACGCTTCATCGAGCTGGTGTCTTAACGAGTTGCTGGAGATTGTGAACTGCAATGATAAAATTATCATACCGGTTTTCTACGGTGTGGATCCTTCCCAAGTGAGGTATCAAATCGGTGACTTTGGAAGGATCTTTGAAAAGACTTGCAAGAGACAAACAGAGGAAGTGAAAAACCAATGGAAGAAAGCGTTGACTCTTGTAGCTAATATGCTTGGATTTGATTCTGCCAAATGGGATGACGAAGCAAAAATGATTGAAGAAATAGCCAATGATGTTTTGCGTAAACTGCTTTTAACTACATCGAAGGATTTTGATGACTTTGTCGGCCTCGAGGATCATATTGCAAATATGAGTGCACTGTTGGATCTGGAATCTAAGGAAGTGAAGATGGTTGGTATATGGGGTTCCTCGGGGATTGGTAAGACTACCATTGCACGAGCTTTGTTTAACAACCTTTTTCGGCATTTCCAAGTTAGGAAATTCATAGACAGATCTTTTGCCTATAAGAGTAGAGAAATTCATAGTAGTGCCAATCCTGACGACCACAATATGAAGTTACATTTACAAGAGAGCTTCCTCTCTGAAATTCTAAGGATGCCGAACATAAAGATAGATGATCCAACTGCACTGGAAGAGAGGCTAAAGTACCAAAAAGTTCTTATCATTATTGATGATTTGGATGATATAATGGTACTAGATACATTGGTGGGTCAAACTCAATGGTTTGGATATGGGAGCAGAATCATTGTGGTTACAAATGATAAGCACTTCTTGACTGCTCATGGGATTGATCATATTTATGAAACAAGGTTATGTGGTGATGCACCGTTCGCTACAAGAAATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

357

Amino Acids

41.15

Weight (kDa)

5.96

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 69 - 241 3.1e-54 TIR domain
TIR_2 PF13676 71 - 164 2e-10 TIR domain
NB-ARC PF00931 255 - 421 1.6e-10 NB-ARC domain
WHD_ROQ1 PF23282 490 - 558 1.2e-21 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 69
AbsI CCTCGAGG 1 cut(s) 554
AclWI GGATC 6 cut(s) 308, 321, 362, 567, 597, 836
AcsI RAATTY 3 cut(s) 704, 741, 810
AcuI CTGAAG 1 cut(s) 37
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 2 cut(s) 872, 918
AfiI CCNNNNNNNGG 1 cut(s) 327
AgeI ACCGGT 1 cut(s) 295
AgsI TTSAA 3 cut(s) 176, 362, 482
AluBI AGCT 5 cut(s) 51, 240, 428, 665, 798
AluI AGCT 5 cut(s) 51, 240, 428, 665, 798
Alw21I GWGCWC 1 cut(s) 583
Alw26I GTCTC 3 cut(s) 57, 126, 370
Alw44I GTGCAC 1 cut(s) 579
AlwI GGATC 6 cut(s) 308, 321, 362, 567, 597, 836
Ama87I CYCGRG 2 cut(s) 554, 634
AoxI GGCC 2 cut(s) 164, 550
ApaLI GTGCAC 1 cut(s) 579
ApoI RAATTY 3 cut(s) 704, 741, 810
ArsI GACNNNNNNTTYG 2 cut(s) 704, 736
AsiGI ACCGGT 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 353, 1055
AvaI CYCGRG 2 cut(s) 554, 634
BaeGI GKGCMC 1 cut(s) 583
BaeI ACNNNNGTAYC 2 cut(s) 915, 948
BamHI GGATCC 1 cut(s) 313
BauI CACGAG 1 cut(s) 660
Bbv12I GWGCWC 1 cut(s) 583
BccI CCATC 1 cut(s) 607
BclI TGATCA 2 cut(s) 117, 1015
BcoDI GTCTC 3 cut(s) 57, 126, 370
BfaI CTAG 1 cut(s) 920
BglII AGATCT 1 cut(s) 716
BmeT110I CYCGRG 2 cut(s) 554, 634
BmgT120I GGNCC 1 cut(s) 165
BmiI GGNNCC 2 cut(s) 315, 631
BmsI GCATC 2 cut(s) 810, 1036
BpmI CTGGAG 2 cut(s) 178, 281
BpuEI CTTGAG 2 cut(s) 67, 80
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 633
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 1 cut(s) 858
Bse3DI GCAATG 2 cut(s) 283, 654
BseDI CCNNGG 1 cut(s) 633
BseGI GGATG 3 cut(s) 466, 825, 910
BseLI CCNNNNNNNGG 1 cut(s) 327
BseMI GCAATG 2 cut(s) 283, 654
BseNI ACTGG 1 cut(s) 858
BseSI GKGCMC 1 cut(s) 583
BsgI GTGCAG 1 cut(s) 834
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 2 cut(s) 166, 552
BshTI ACCGGT 1 cut(s) 295
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 583
BsiHKCI CYCGRG 2 cut(s) 554, 634
BsiSI CCGG 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 327
BsmAI GTCTC 3 cut(s) 57, 126, 370
BsmBI CGTCTC 1 cut(s) 57
BsnI GGCC 2 cut(s) 166, 552
BsoBI CYCGRG 2 cut(s) 554, 634
Bsp1286I GDGCHC 1 cut(s) 583
BspANI GGCC 2 cut(s) 166, 552
BspLI GGNNCC 2 cut(s) 315, 631
BspPI GGATC 6 cut(s) 308, 321, 362, 567, 597, 836
BsrDI GCAATG 2 cut(s) 283, 654
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 1 cut(s) 858
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 633
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 660
Bst2BI CACGAG 1 cut(s) 660
Bst4CI ACNGT 3 cut(s) 308, 585, 1053
Bst6I CTCTTC 1 cut(s) 852
BstACI GRCGYC 1 cut(s) 66
BstDEI CTNAG 2 cut(s) 600, 815
BstF5I GGATG 3 cut(s) 466, 825, 910
BstMAI GTCTC 3 cut(s) 57, 126, 370
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 2 cut(s) 511, 1055
BstSLI GKGCMC 1 cut(s) 583
BstX2I RGATCY 4 cut(s) 313, 354, 589, 716
BstYI RGATCY 4 cut(s) 313, 354, 589, 716
BsuRI GGCC 2 cut(s) 166, 552
BtsCI GGATG 3 cut(s) 466, 825, 910
BtsI GCAGTG 2 cut(s) 120, 210
BtsIMutI CAGTG 4 cut(s) 120, 210, 581, 851
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 165
Csp6I GTAC 2 cut(s) 871, 917
CspAI ACCGGT 1 cut(s) 295
CspCI CAANNNNNGTGG 2 cut(s) 143, 178
CviAII CATG 1 cut(s) 1007
CviQI GTAC 2 cut(s) 871, 917
DdeI CTNAG 2 cut(s) 600, 815
Eam1104I CTCTTC 1 cut(s) 852
EarI CTCTTC 1 cut(s) 852
Eco57I CTGAAG 1 cut(s) 37
Eco88I CYCGRG 2 cut(s) 554, 634
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 1 cut(s) 1010
FalI AAGNNNNNCTT 2 cut(s) 980, 1012
FatI CATG 1 cut(s) 1006
FbaI TGATCA 2 cut(s) 117, 1015
FokI GGATG 3 cut(s) 473, 832, 917
FspBI CTAG 1 cut(s) 920
GsuI CTGGAG 2 cut(s) 178, 281
HaeIII GGCC 2 cut(s) 166, 552
HapII CCGG 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 1 cut(s) 1010
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HinfI GANTC 6 cut(s) 191, 198, 418, 446, 596, 966
HpaII CCGG 1 cut(s) 296
HphI GGTGA 2 cut(s) 353, 1055
Hpy166II GTNNAC 4 cut(s) 271, 417, 509, 581
Hpy188I TCNGA 4 cut(s) 56, 111, 131, 808
Hpy188III TCNNGA 8 cut(s) 21, 46, 145, 173, 195, 593, 761, 997
Hpy8I GTNNAC 4 cut(s) 271, 417, 509, 581
HpyAV CCTTC 3 cut(s) 327, 345, 523
HpyCH4III ACNGT 3 cut(s) 308, 585, 1053
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 7 cut(s) 276, 372, 569, 581, 659, 851, 1049
HpyF10VI GCNNNNNNNGC 2 cut(s) 511, 1055
HpyF3I CTNAG 2 cut(s) 600, 815
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 1 cut(s) 1010
Ksp22I TGATCA 2 cut(s) 117, 1015
LmnI GCTCC 1 cut(s) 960
LpnPI CCDG 7 cut(s) 208, 226, 245, 309, 578, 774, 839
LweI GCATC 2 cut(s) 810, 1036
MaeI CTAG 1 cut(s) 920
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 4 cut(s) 73, 341, 780, 976
MboII GAAGA 6 cut(s) 6, 9, 418, 494, 622, 869
MflI RGATCY 4 cut(s) 313, 354, 589, 716
MhlI GDGCHC 1 cut(s) 583
MluCI AATT 5 cut(s) 25, 285, 704, 741, 810
MlyI GAGTC 2 cut(s) 185, 412
MmeI TCCRAC 2 cut(s) 567, 869
MnlI CCTC 8 cut(s) 50, 321, 379, 550, 563, 643, 812, 855
MseI TTAA 3 cut(s) 249, 518, 672
MslI CAYNNNNRTG 2 cut(s) 750, 1023
MspI CCGG 1 cut(s) 296
MwoI GCNNNNNNNGC 2 cut(s) 511, 1055
NlaIII CATG 1 cut(s) 1010
NlaIV GGNNCC 2 cut(s) 315, 631
NmuCI GTSAC 1 cut(s) 341
PaeR7I CTCGAG 1 cut(s) 554
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 4 cut(s) 198, 446, 596, 966
PflFI GACNNNGTC 1 cut(s) 545
PinAI ACCGGT 1 cut(s) 295
Ple19I CGATCG 1 cut(s) 109
PleI GAGTC 2 cut(s) 185, 412
PpsI GAGTC 2 cut(s) 185, 412
PspN4I GGNNCC 2 cut(s) 315, 631
PspPI GGNCC 1 cut(s) 165
PspXI VCTCGAGB 1 cut(s) 554
PsrI GAACNNNNNNTAC 2 cut(s) 613, 645
PsuI RGATCY 4 cut(s) 313, 354, 589, 716
PsyI GACNNNGTC 1 cut(s) 545
PvuI CGATCG 1 cut(s) 109
RsaI GTAC 2 cut(s) 872, 918
RsaNI GTAC 2 cut(s) 871, 917
RseI CAYNNNNRTG 2 cut(s) 750, 1023
SaqAI TTAA 3 cut(s) 249, 518, 672
Sau96I GGNCC 1 cut(s) 165
SchI GAGTC 2 cut(s) 185, 412
SduI GDGCHC 1 cut(s) 583
SetI ASST 9 cut(s) 53, 69, 242, 332, 430, 667, 681, 800, 1037
SfaNI GCATC 2 cut(s) 810, 1036
Sfr274I CTCGAG 1 cut(s) 554
SlaI CTCGAG 1 cut(s) 554
SmiMI CAYNNNNRTG 2 cut(s) 750, 1023
SmlI CTYRAG 3 cut(s) 46, 95, 554
SmoI CTYRAG 3 cut(s) 46, 95, 554
Sse9I AATT 5 cut(s) 25, 285, 704, 741, 810
SspMI CTAG 1 cut(s) 920
TaaI ACNGT 3 cut(s) 308, 585, 1053
TaiI ACGT 1 cut(s) 69
TaqI TCGA 6 cut(s) 22, 105, 144, 236, 527, 555
TasI AATT 5 cut(s) 25, 285, 704, 741, 810
TfiI GAWTC 4 cut(s) 198, 446, 596, 966
Tru1I TTAA 3 cut(s) 249, 518, 672
Tru9I TTAA 3 cut(s) 249, 518, 672
TscAI CASTG 4 cut(s) 127, 210, 588, 858
TseFI GTSAC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 5 cut(s) 222, 697, 734, 791, 1041
TspGWI ACGGA 2 cut(s) 59, 71
TspRI CASTG 4 cut(s) 127, 210, 588, 858
Tth111I GACNNNGTC 1 cut(s) 545
VneI GTGCAC 1 cut(s) 579
XapI RAATTY 3 cut(s) 704, 741, 810
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XhoI CTCGAG 1 cut(s) 554
XspI CTAG 1 cut(s) 920
ZraI GACGTC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.