AT4G08450

TIR domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002912
Physical Location & Seq
Forward (+)
5365501 .. 5371211
5711 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08450.2

Sequence Viewer

Length: 3705 bp
ATGGCTTCTTCTTCTTCTCATAACTGGGTTTACGACGTTTTCACAAGCTTCAGCGGGGAAGATATCCGTGTAACTTTCCTCACCCACTTTCTCAAGGAGCTTGATCGAAAAATGATTATTGCTTTCAAAGACAATGAGATTGAGAGAGGGAACTCGATCGGTACCGAGCTTATACAGGCGATAAAGGATTCGAGGATCGCGGTGGTTGTGTTCTCCAAAAAATACTCTTCTTCGAGTTGGTGTTTGAATGAGTTGGTAGAGATTGTGAATTGCAAGGAAATTGTGATACCAGTTTTCTATGATTTAGATCCTTCTGATGTTAGGAAACAAGAGGGAGAATTTGGAGAGTCTTTTAAAGAGACTTGCAAGAACAGAACAGATTATGAGATACAACGTTGGGGCCAAGCGTTGACCAATGTAGCAAATATTGCTGGATATCACACTAGGAAACCGAATAACGAAGCAAAATTGATTGAAGAAATCACTAATGATGTTTTGGATAAACTAATGAAGTTAACACCATCGAAAGATTTTGATGAATTTTTTGGCATCGAAGATCATATCAAAGAGCTGAGTTTATTATTGTGCTTGGAATCTGAGGAAGTGAGAATGGTTGGGATATGGGGTCCTACGGGGATTGGTAAGACAACCATTGCAAGAGCTTTATTTAATCGAATCTACCGTCACTTCCAAGGTAGGGTTTTTATAGACAGAGCTTTTATATCTAAGAGTATGGCTATTTATAGTAGAGCAAACTCGGACGACTATAACTTGAAGTTGCATTTGCAAGAAAAGTTGTTGTCTAAACTTTTAGACAAAAAAAACTTGGAGATAAATCACCTAGATGCTGTGAAAGAGAGGCTAAGGCAAATGAAAGTTCTTATCTTTATTGATGATTTGGATGATCAAGTGGTGCTAGAAGCCTTAGCCTGTCAAACTCAGTGGTTTGGGCATGGAAGTAGAATCATTGTGATTACAAAAGATAAGCATCTCTTAAGGGCTTATGGGATTGATCATATATATGAGGTTCTTCTCCCGTCGAAAGACCTTGCTATTAAGATGTTTTGTCGATCTGCTTTTAGGAAAGACTCTCCACCCAATGGTTTTATAGAACTTGCTTATGATGTTGTAAAACGTGCAGGTAGTCTTCCTTTGGGTCTTAACATTTTAGGTTCGTATTTACGGGGTAGGAGTAAGGAGGATTGGATAGATATGATGCCGGGGCTTCGTAATAAACTAGACGGGAAAATTCAGAAAACACTTAGAGTCAGCTACGACGGGTTGGCTAGCGAAGACGATCAAGCGATATTTCGTCATATTGCATGTATTTTTAATTTTGAGGCATGCAGTGACATCAAGAAACTGCTTGAAGATAGTGGCTTGAATGTCACTAATGGGCTTATAAACCTTGTCGATAAGTCCCTCATACGTATAGAACCAAAGCAGAAAACTGTGGAGATGCATTGTTTGTTACAAGAAACGGCTAGAGAAATTATTCGTGCACAGTCTTTTGATGATCCTGGAAAACGAGAATTCCTTGTGGATGGGAAGGATATTGCTGATGTCCTAGATAATTGCAGTGGTACAAGAAAAGTCTTAGGTATATCATTGGATATGGATGAGATTGAAGAGTTGCATCTACAAGTAGACGCCTTCAAAAAGATGCTTAATCTCCGTTTTCTAAAACTTTACACAAATACAAATATATCAGAAAAAGAAGACAAATTGCTCTTACCTAAAGAATTCAACTATTTACCCAATACACTCAGATTACTAAGTTGGCAAAGGTTTCCAATGAGATGTATGCCTTCTGATTTTTTTCCTAAATATCTTGTTAAACTTCTAATGCCGGGGAGCAAGCTCGAGAAGCTGTGGGACGGAGTAATGCCGCTTCAATGCCTCAAGAATATGAATTTGTTTGGATCTGAAAACCTTAAAGAATTCCCAAATCTCTCGCTAGCAACAAACCTAGAGACATTGTCTCTTGGCTTTTGCTTGAGTTTGGTTGAGGTTCCTTCCACTATTGGGAATCTCAATAAACTTACGTATTTGAACATGTCAGGATGCCATAATTTGGAGAAATTTCCGGCTGATGTCAACCTCAAATCTCTCTCTGACCTCGTTCTCAATGGATGCTCACGTTTGAAGATTTTTCCCGCTATATCAAGCAACATCTCAGAGCTATGCCTAAACAGTTTAGCAGTTGAGGAATTCCCATCCAACTTGCATCTAGAGAATCTTGTTTATCTTTTAATTTGGGGAATGACGAGTGTCAAGTTGTGGGATGGCGTGAAGGTGCTTACATCCCTTAAAACTATGCATTTGCGAGATTCTAAAAACCTGAAAGAAATCCCAGATCTTTCAATGGCATCGAATCTTCTGATACTAAATCTTGAACAATGCATAAGTATTGTGGAGCTTCCTTCCTCTATTAGAAATCTCCATAACTTGATAGAGTTGGACATGTCAGGATGCACAAACCTGGAGACTTTTCCAACTGGCATCAACCTTCAATCTCTAAAACGCATAAATCTAGCTAGATGCTCACGGTTAAAGATTTTTCCGGATATCTCAACCAACATCTCAGAGCTTGATCTAAGCCAAACAGCGATTGAAGAAGTTCCTTTGTGGATCGAGAACTTCTCTAAGCTCAAATACTTAATAATGGGAAAATGTAACATGTTGGAATATGTATTCCTAAACATTTCCAAACTGAAACATCTCAAGAGCGTTGACTTTTCAGACTGTGGGATATTGTCTAAAGCAGACATGTACATGCTACAAGTACCCAATGAAGCTTCCTCTTCACTTCCAATTAATTGTGTCCAAAAAGCTGAGCTTATATTCATCAACTGCTACAAGCTGAATCAAAAAGCTCTCATTCGCCAACAATTTTTTCTGAAGAAAATGATTTTGCCAGGTGAAGAAGTGCCTTTTTATTTCACTCACCAAACAATAGGAAGCTCTATTGGTATCCCTCTACTTCACATATTACTTTCTCAACAATACTTCAGATTTAAGGCTTGTGTTGTGGTTGATCCCAAGTTTGTGTTCCCAGCTCGTCGTTATCACGTAAACATCCAGGTTTCTTGTCGATTCAAAGGCATATATGGGAACTACTTTGATTATGCTGATCAGCCACATTGCTTCTCACCATCTCAGACGGACAATTATGTGTATGTATTTGATTGTTGTTTCCCTCTTAACAAAGACAATGCTCCTCTAGCTGAACTCGACTATGATCATGTGGATATAGAATTTCATCTTGATGATAACTACAATCACCACAAAATAAAAGGATGCGGTATACGACTCTCTGAGGATGATGAAAGCAGTGACGATGATGATGACCTAAGCAGTGAAACTGATTATAGTGATGAGTGTGAAGATAGCGATGATAGTGATCTTGGAAATGAAATTGATTACAGTGAAGACTATGAAGACCGCGATACTAGTGATCTTGGAAATGAAACTGATTACAGTGAAGAGTATGAAGACCATGATAGTAGTGATCTTGGCAATGAGTATGACTATAATGAAGAGTGTGAAGAAAATGATGATAATGATCTTGTACTTGAGACTGATCACAATGAAGAGTGTCAAGACAACGATGTGGAGAAGAAGAGAAGTAGTAAGCGAATGCGGGTAAATTTTTATATAGTTCACAATTCTTTTCCAAGATCAAACGTAAACTTTTTCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1234

Amino Acids

141.67

Weight (kDa)

5.11

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 183 2.2e-54 TIR domain
TIR_2 PF13676 13 - 104 2.1e-10 TIR domain
AAA_16 PF13191 181 - 303 1.5e-06 AAA ATPase domain
NB-ARC PF00931 188 - 363 8.6e-13 NB-ARC domain
WHD_ROQ1 PF23282 431 - 503 2.6e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 610 - 628 2.7e-06 Leucine Rich Repeat
C-JID PF20160 972 - 1106 4.6e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1403
Acc36I ACCTGC 1 cut(s) 1130
Acc65I GGTACC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 161
AccB7I CCANNNNNTGG 2 cut(s) 1100, 2074
AccI GTMKAC 2 cut(s) 1647, 3307
AccII CGCG 2 cut(s) 200, 3447
AccIII TCCGGA 1 cut(s) 2563
AciI CCGC 7 cut(s) 54, 200, 1889, 2157, 3303, 3445, 3643
AclI AACGTT 1 cut(s) 394
AclWI GGATC 6 cut(s) 203, 302, 1511, 1930, 2639, 3032
AcuI CTGAAG 3 cut(s) 34, 2921, 2995
AcyI GRCGYC 1 cut(s) 1650
AfaI GTAC 5 cut(s) 163, 1585, 2774, 2787, 3573
AfiI CCNNNNNNNGG 4 cut(s) 697, 1100, 2025, 2074
AflII CTTAAG 1 cut(s) 994
AflIII ACRYGT 4 cut(s) 2055, 2463, 2679, 2769
AhlI ACTAGT 1 cut(s) 3452
AjnI CCWGG 4 cut(s) 1519, 2481, 2917, 3081
AjuI GAANNNNNNNTTGG 4 cut(s) 2880, 2912, 3035, 3067
Alw21I GWGCWC 1 cut(s) 1504
Alw26I GTCTC 5 cut(s) 353, 1967, 1986, 2480, 3572
Alw44I GTGCAC 1 cut(s) 1500
AlwI GGATC 6 cut(s) 203, 302, 1511, 1930, 2639, 3032
Ama87I CYCGRG 1 cut(s) 1862
Aor13HI TCCGGA 1 cut(s) 2563
AoxI GGCC 1 cut(s) 400
ApaLI GTGCAC 1 cut(s) 1500
ArsI GACNNNNNNTTYG 2 cut(s) 667, 699
AseI ATTAAT 1 cut(s) 2817
Asp700I GAANNNNTTC 2 cut(s) 1494, 2619
Asp718I GGTACC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 400, 626
AsuC2I CCSGG 2 cut(s) 1221, 1851
AsuHPI GGTGA 6 cut(s) 73, 830, 2933, 2939, 3144, 3275
AsuNHI GCTAGC 2 cut(s) 1286, 1957
AvaI CYCGRG 1 cut(s) 1862
AvaII GGWCC 1 cut(s) 626
BaeGI GKGCMC 1 cut(s) 1504
BanI GGYRCC 1 cut(s) 161
BarI GAAGNNNNNNTAC 2 cut(s) 1875, 1907
BbsI GAAGAC 6 cut(s) 1139, 1299, 1725, 3438, 3447, 3501
Bbv12I GWGCWC 1 cut(s) 1504
BccI CCATC 5 cut(s) 529, 1538, 2224, 2279, 3163
BceAI ACGGC 1 cut(s) 1497
BciT130I CCWGG 4 cut(s) 1521, 2483, 2919, 3083
BciVI GTATCC 1 cut(s) 2984
BclI TGATCA 5 cut(s) 904, 1012, 3133, 3241, 3583
BcnI CCSGG 2 cut(s) 1221, 1851
BcoDI GTCTC 5 cut(s) 353, 1967, 1986, 2480, 3572
BcuI ACTAGT 1 cut(s) 3452
BfrI CTTAAG 1 cut(s) 994
BfuAI ACCTGC 1 cut(s) 1130
BfuI GTATCC 1 cut(s) 2984
BglII AGATCT 1 cut(s) 2356
BisI GCNGC 1 cut(s) 1889
BlpI GCTNAGC 1 cut(s) 2835
BlsI GCNGC 1 cut(s) 1890
Bme1390I CCNGG 6 cut(s) 1221, 1521, 1851, 2483, 2919, 3083
Bme18I GGWCC 1 cut(s) 626
BmeT110I CYCGRG 1 cut(s) 1862
BmgT120I GGNCC 2 cut(s) 400, 626
BmiI GGNNCC 4 cut(s) 163, 401, 627, 2013
BmrFI CCNGG 6 cut(s) 1221, 1521, 1851, 2483, 2919, 3083
BmrI ACTGGG 1 cut(s) 34
BmtI GCTAGC 2 cut(s) 1290, 1961
BmuI ACTGGG 1 cut(s) 34
BoxI GACNNNNGTC 1 cut(s) 2270
BpiI GAAGAC 6 cut(s) 1139, 1299, 1725, 3438, 3447, 3501
BplI GAGNNNNNCTC 2 cut(s) 2627, 2659
BpmI CTGGAG 1 cut(s) 2504
Bpu10I CCTNAGC 3 cut(s) 863, 925, 3353
Bpu1102I GCTNAGC 1 cut(s) 2835
BpuEI CTTGAG 5 cut(s) 77, 1886, 2017, 2708, 3596
BpuMI CCSGG 2 cut(s) 1221, 1851
BsaAI YACGTR 3 cut(s) 1430, 2046, 3073
BsaBI GATNNNNATC 4 cut(s) 306, 987, 3402, 3564
BsaHI GRCGYC 1 cut(s) 1650
BsaJI CCNNGG 3 cut(s) 691, 1220, 1850
BsaWI WCCGGW 1 cut(s) 2563
Bsc4I CCNNNNNNNGG 4 cut(s) 697, 1100, 2025, 2074
Bse1I ACTGG 3 cut(s) 29, 290, 2503
Bse3DI GCAATG 3 cut(s) 651, 3142, 3526
Bse8I GATNNNNATC 4 cut(s) 306, 987, 3402, 3564
BseAI TCCGGA 1 cut(s) 2563
BseBI CCWGG 4 cut(s) 1521, 2483, 2919, 3083
BseDI CCNNGG 3 cut(s) 691, 1220, 1850
BseJI GATNNNNATC 4 cut(s) 306, 987, 3402, 3564
BseLI CCNNNNNNNGG 4 cut(s) 697, 1100, 2025, 2074
BseMI GCAATG 3 cut(s) 651, 3142, 3526
BseMII CTCAG 9 cut(s) 563, 588, 953, 1780, 2190, 2598, 2826, 3173, 3309
BseNI ACTGG 3 cut(s) 29, 290, 2503
BseRI GAGGAG 1 cut(s) 3210
BseSI GKGCMC 1 cut(s) 1504
BseYI CCCAGC 1 cut(s) 3055
BsgI GTGCAG 1 cut(s) 1158
Bsh1236I CGCG 2 cut(s) 200, 3447
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 1 cut(s) 402
BshNI GGYRCC 1 cut(s) 161
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 1504
BsiHKCI CYCGRG 1 cut(s) 1862
BsiSI CCGG 4 cut(s) 1220, 1850, 2087, 2564
BslFI GGGAC 2 cut(s) 1405, 1889
BslI CCNNNNNNNGG 4 cut(s) 697, 1100, 2025, 2074
BsmAI GTCTC 5 cut(s) 353, 1967, 1986, 2480, 3572
BsmFI GGGAC 2 cut(s) 1405, 1889
BsmI GAATGC 1 cut(s) 3645
BsnI GGCC 1 cut(s) 402
BsoBI CYCGRG 1 cut(s) 1862
Bsp1286I GDGCHC 1 cut(s) 1504
Bsp13I TCCGGA 1 cut(s) 2563
Bsp1407I TGTACA 1 cut(s) 2772
Bsp1720I GCTNAGC 1 cut(s) 2835
BspACI CCGC 7 cut(s) 54, 200, 1889, 2157, 3303, 3445, 3643
BspANI GGCC 1 cut(s) 402
BspCNI CTCAG 9 cut(s) 564, 589, 952, 1779, 2189, 2597, 2827, 3172, 3310
BspEI TCCGGA 1 cut(s) 2563
BspFNI CGCG 2 cut(s) 200, 3447
BspLI GGNNCC 4 cut(s) 163, 401, 627, 2013
BspMI ACCTGC 1 cut(s) 1130
BspOI GCTAGC 2 cut(s) 1290, 1961
BspPI GGATC 6 cut(s) 203, 302, 1511, 1930, 2639, 3032
BspT107I GGYRCC 1 cut(s) 161
BspTI CTTAAG 1 cut(s) 994
BsrDI GCAATG 3 cut(s) 651, 3142, 3526
BsrGI TGTACA 1 cut(s) 2772
BsrI ACTGG 3 cut(s) 29, 290, 2503
BssECI CCNNGG 3 cut(s) 691, 1220, 1850
BssNAI GTATAC 1 cut(s) 3308
BssNI GRCGYC 1 cut(s) 1650
BssT1I CCWWGG 1 cut(s) 691
Bst1107I GTATAC 1 cut(s) 3308
Bst2UI CCWGG 4 cut(s) 1521, 2483, 2919, 3083
Bst4CI ACNGT 8 cut(s) 683, 1453, 1506, 2195, 2550, 2747, 3428, 3482
Bst6I CTCTTC 7 cut(s) 232, 1623, 2809, 3480, 3534, 3588, 3617
BstACI GRCGYC 1 cut(s) 1650
BstAFI CTTAAG 1 cut(s) 994
BstAPI GCANNNNNTGC 1 cut(s) 428
BstAUI TGTACA 1 cut(s) 2772
BstBAI YACGTR 3 cut(s) 1430, 2046, 3073
BstC8I GCNNGC 4 cut(s) 1288, 1345, 1859, 1959
BstFNI CGCG 2 cut(s) 200, 3447
BstMAI GTCTC 5 cut(s) 353, 1967, 1986, 2480, 3572
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 3 cut(s) 428, 1867, 3224
BstNI CCWGG 4 cut(s) 1521, 2483, 2919, 3083
BstNSI RCATGY 7 cut(s) 1326, 1347, 2059, 2467, 2683, 2773, 2779
BstPAI GACNNNNGTC 1 cut(s) 2270
BstSCI CCNGG 6 cut(s) 1219, 1519, 1849, 2481, 2917, 3081
BstSLI GKGCMC 1 cut(s) 1504
BstSNI TACGTA 2 cut(s) 1430, 2046
BstUI CGCG 2 cut(s) 200, 3447
BstV2I GAAGAC 6 cut(s) 1139, 1299, 1725, 3438, 3447, 3501
BstX2I RGATCY 3 cut(s) 307, 1922, 2356
BstYI RGATCY 3 cut(s) 307, 1922, 2356
BstZ17I GTATAC 1 cut(s) 3308
BsuI GTATCC 1 cut(s) 2984
BsuRI GGCC 1 cut(s) 402
BtgZI GCGATG 1 cut(s) 3408
BtsI GCAGTG 4 cut(s) 1354, 1585, 3340, 3364
BtsIMutI CAGTG 7 cut(s) 947, 1354, 1585, 3340, 3364, 3433, 3487
BveI ACCTGC 1 cut(s) 1130
Cac8I GCNNGC 4 cut(s) 1288, 1345, 1859, 1959
Cfr13I GGNCC 2 cut(s) 400, 626
CseI GACGC 1 cut(s) 1658
Csp6I GTAC 5 cut(s) 162, 1584, 2773, 2786, 3572
CspCI CAANNNNNGTGG 2 cut(s) 923, 958
CviQI GTAC 5 cut(s) 162, 1584, 2773, 2786, 3572
DraI TTTAAA 1 cut(s) 355
Eam1104I CTCTTC 7 cut(s) 232, 1623, 2809, 3480, 3534, 3588, 3617
EarI CTCTTC 7 cut(s) 232, 1623, 2809, 3480, 3534, 3588, 3617
Eco105I TACGTA 2 cut(s) 1430, 2046
Eco130I CCWWGG 1 cut(s) 691
Eco32I GATATC 3 cut(s) 64, 437, 2569
Eco47I GGWCC 1 cut(s) 626
Eco57I CTGAAG 3 cut(s) 34, 2921, 2995
Eco88I CYCGRG 1 cut(s) 1862
EcoO109I RGGNCCY 1 cut(s) 626
EcoRI GAATTC 4 cut(s) 1532, 1742, 1940, 2210
EcoRII CCWGG 4 cut(s) 1519, 2481, 2917, 3081
EcoRV GATATC 3 cut(s) 64, 437, 2569
EcoT14I CCWWGG 1 cut(s) 691
EcoT22I ATGCAT 3 cut(s) 1464, 2322, 2405
ErhI CCWWGG 1 cut(s) 691
FalI AAGNNNNNCTT 4 cut(s) 977, 1009, 2823, 2855
FaqI GGGAC 2 cut(s) 1405, 1889
FauI CCCGC 3 cut(s) 47, 2164, 3636
FbaI TGATCA 5 cut(s) 904, 1012, 3133, 3241, 3583
FblI GTMKAC 2 cut(s) 1647, 3307
Fnu4HI GCNGC 1 cut(s) 1889
Fsp4HI GCNGC 1 cut(s) 1889
GluI GCNGC 1 cut(s) 1889
GsaI CCCAGC 1 cut(s) 3059
GsuI CTGGAG 1 cut(s) 2504
HaeIII GGCC 1 cut(s) 402
HapII CCGG 4 cut(s) 1220, 1850, 2087, 2564
HgaI GACGC 1 cut(s) 1658
Hin1I GRCGYC 1 cut(s) 1650
HincII GTYRAC 4 cut(s) 411, 516, 2098, 2734
HindII GTYRAC 4 cut(s) 411, 516, 2098, 2734
HindIII AAGCTT 2 cut(s) 46, 2796
HpaI GTTAAC 1 cut(s) 516
HpaII CCGG 4 cut(s) 1220, 1850, 2087, 2564
HphI GGTGA 6 cut(s) 73, 830, 2933, 2939, 3144, 3275
Hpy99I CGWCG 4 cut(s) 38, 1042, 1280, 3066
HpyAV CCTTC 8 cut(s) 321, 1543, 1663, 1818, 2025, 2287, 2433, 2519
HpyCH4III ACNGT 8 cut(s) 683, 1453, 1506, 2195, 2550, 2747, 3428, 3482
HpyCH4IV ACGT 8 cut(s) 36, 394, 1135, 1429, 2045, 2140, 3072, 3687
HpyF10VI GCNNNNNNNGC 3 cut(s) 428, 1867, 3224
HpySE526I ACGT 8 cut(s) 36, 394, 1135, 1429, 2045, 2140, 3072, 3687
Hsp92I GRCGYC 1 cut(s) 1650
Kpn2I TCCGGA 1 cut(s) 2563
KpnI GGTACC 1 cut(s) 165
Ksp22I TGATCA 5 cut(s) 904, 1012, 3133, 3241, 3583
KspAI GTTAAC 1 cut(s) 516
LmnI GCTCC 4 cut(s) 97, 1854, 2416, 3223
MaeII ACGT 8 cut(s) 36, 394, 1135, 1429, 2045, 2140, 3072, 3687
MaeIII GTNAC 7 cut(s) 70, 683, 1349, 1387, 1470, 2675, 3335
MflI RGATCY 3 cut(s) 307, 1922, 2356
MhlI GDGCHC 1 cut(s) 1504
MlyI GAGTC 4 cut(s) 356, 1082, 1275, 3306
MmeI TCCRAC 4 cut(s) 2244, 2439, 2519, 2664
Mph1103I ATGCAT 3 cut(s) 1464, 2322, 2405
MroI TCCGGA 1 cut(s) 2563
MroXI GAANNNNTTC 2 cut(s) 1494, 2619
MslI CAYNNNNRTG 4 cut(s) 843, 1020, 2774, 3267
MspA1I CMGCKG 1 cut(s) 54
MspCI CTTAAG 1 cut(s) 994
MspI CCGG 4 cut(s) 1220, 1850, 2087, 2564
MspR9I CCNGG 6 cut(s) 1221, 1521, 1851, 2483, 2919, 3083
Mva1269I GAATGC 1 cut(s) 3645
MvaI CCWGG 4 cut(s) 1521, 2483, 2919, 3083
MvnI CGCG 2 cut(s) 200, 3447
MwoI GCNNNNNNNGC 3 cut(s) 428, 1867, 3224
NciI CCSGG 2 cut(s) 1221, 1851
NheI GCTAGC 2 cut(s) 1286, 1957
NlaIV GGNNCC 4 cut(s) 163, 401, 627, 2013
NmuCI GTSAC 4 cut(s) 683, 1349, 1387, 3335
NsiI ATGCAT 3 cut(s) 1464, 2322, 2405
NspI RCATGY 7 cut(s) 1326, 1347, 2059, 2467, 2683, 2773, 2779
PaeI GCATGC 1 cut(s) 1347
PaeR7I CTCGAG 1 cut(s) 1862
PciI ACATGT 4 cut(s) 2055, 2463, 2679, 2769
PcsI WCGNNNNNNNCGW 1 cut(s) 679
PctI GAATGC 1 cut(s) 3645
PdmI GAANNNNTTC 2 cut(s) 1494, 2619
PflFI GACNNNGTC 1 cut(s) 1978
PflMI CCANNNNNTGG 2 cut(s) 1100, 2074
PfoI TCCNGGA 1 cut(s) 1519
PkrI GCNGC 1 cut(s) 1890
Ple19I CGATCG 1 cut(s) 159
PleI GAGTC 4 cut(s) 355, 1082, 1274, 3306
PpsI GAGTC 4 cut(s) 355, 1082, 1274, 3306
Ppu21I YACGTR 3 cut(s) 1430, 2046, 3073
PpuMI RGGWCCY 1 cut(s) 626
PscI ACATGT 4 cut(s) 2055, 2463, 2679, 2769
PshAI GACNNNNGTC 1 cut(s) 2270
PshBI ATTAAT 1 cut(s) 2817
PsiI TTATAA 1 cut(s) 1403
Psp1406I AACGTT 1 cut(s) 394
Psp5II RGGWCCY 1 cut(s) 626
Psp6I CCWGG 4 cut(s) 1519, 2481, 2917, 3081
PspFI CCCAGC 1 cut(s) 3055
PspGI CCWGG 4 cut(s) 1519, 2481, 2917, 3081
PspN4I GGNNCC 4 cut(s) 163, 401, 627, 2013
PspPI GGNCC 2 cut(s) 400, 626
PspPPI RGGWCCY 1 cut(s) 626
PsuI RGATCY 3 cut(s) 307, 1922, 2356
PsyI GACNNNGTC 1 cut(s) 1978
PvuI CGATCG 1 cut(s) 159
RsaI GTAC 5 cut(s) 163, 1585, 2774, 2787, 3573
RsaNI GTAC 5 cut(s) 162, 1584, 2773, 2786, 3572
RseI CAYNNNNRTG 4 cut(s) 843, 1020, 2774, 3267
SatI GCNGC 1 cut(s) 1889
Sau96I GGNCC 2 cut(s) 400, 626
SchI GAGTC 4 cut(s) 356, 1082, 1275, 3306
ScrFI CCNGG 6 cut(s) 1221, 1521, 1851, 2483, 2919, 3083
SduI GDGCHC 1 cut(s) 1504
Sfr274I CTCGAG 1 cut(s) 1862
SinI GGWCC 1 cut(s) 626
SlaI CTCGAG 1 cut(s) 1862
SmiMI CAYNNNNRTG 4 cut(s) 843, 1020, 2774, 3267
SmlI CTYRAG 7 cut(s) 92, 994, 1862, 1901, 1996, 2723, 3575
SmoI CTYRAG 7 cut(s) 92, 994, 1862, 1901, 1996, 2723, 3575
SnaBI TACGTA 2 cut(s) 1430, 2046
SpeI ACTAGT 1 cut(s) 3452
SphI GCATGC 1 cut(s) 1347
SsiI CCGC 7 cut(s) 54, 200, 1889, 2157, 3303, 3445, 3643
SspI AATATT 1 cut(s) 427
StyD4I CCNGG 6 cut(s) 1219, 1519, 1849, 2481, 2917, 3081
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 8 cut(s) 683, 1453, 1506, 2195, 2550, 2747, 3428, 3482
TaiI ACGT 8 cut(s) 39, 397, 1138, 1432, 2048, 2143, 3075, 3690
TatI WGTACW 2 cut(s) 2772, 3571
TauI GCSGC 1 cut(s) 1891
TscAI CASTG 7 cut(s) 947, 1354, 1585, 3340, 3364, 3433, 3487
TseFI GTSAC 4 cut(s) 683, 1349, 1387, 3335
Tsp45I GTSAC 4 cut(s) 683, 1349, 1387, 3335
TspGWI ACGGA 4 cut(s) 56, 1664, 1893, 3179
TspRI CASTG 7 cut(s) 947, 1354, 1585, 3340, 3364, 3433, 3487
Tth111I GACNNNGTC 1 cut(s) 1978
Van91I CCANNNNNTGG 2 cut(s) 1100, 2074
Vha464I CTTAAG 1 cut(s) 994
VneI GTGCAC 1 cut(s) 1500
VpaK11BI GGWCC 1 cut(s) 626
VspI ATTAAT 1 cut(s) 2817
XbaI TCTAGA 1 cut(s) 2230
XceI RCATGY 7 cut(s) 1326, 1347, 2059, 2467, 2683, 2773, 2779
XhoI CTCGAG 1 cut(s) 1862
XmiI GTMKAC 2 cut(s) 1647, 3307
XmnI GAANNNNTTC 2 cut(s) 1494, 2619
Zsp2I ATGCAT 3 cut(s) 1464, 2322, 2405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.