AT5G46500

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002912
Physical Location & Seq
Forward (+)
18855955 .. 18858552
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46500.2

Sequence Viewer

Length: 1254 bp
ATGATGTTGTCTCCCACTTTGACGAGCCATTGCAGCAGGCTAAAATGTGTATCCCTACACATTTCTAAACTGAAACATCTTGAGGACGCTTTGTTTCCAGCTTGTGGGGCATTAAATCGAGTTGAATTGAGTGGTTCTTCTAGTGGGATGAAAGCAGACAATATTGACACAGCCTCCTCTTCTCTTCCTCAAGTCGAACTTGATTTCAGGGAGTGCTTCAACTTGGATCCAGAAACTGTCCTTCACCAAGAATCAATTATATTCAAGTACATGTTATTTCCAGGGAAAGAAGAAGTGCCATCATATTTCACTTACCGTACTACTGGAGTCTCCTCTCTGACCATTCCTCTACTTCACCTCCCTCTCTCCCAACCATTCTTCAGATTTAGGGTTGGCGCATTGGTAACTAATGTTAAGCATGGAAAAAATATAAAGGTAAAATGTGAGTTCAAAGACAGATTTGGGAACAGCTTTCATGTTGGCTCGGATGATTTCTACGTTTATCTACTTTTCACGAAAAGTCAGAAGGGTAGTCAAATGTTAACTATATTGGACTGTTGTATCCCTCTAAACGAAGGTAATGCTTCTCTAGCTCAAGGGAACTACTACGATCATGTGGATATAAATATTCATATAACAGAATCTCTTGGATCTTTTGGATCTACGTCTGAATTAAAAGAATGGGGTATACGACTCTTAGAGGAAGACTCTTCATCAGCAGAGAACCAACTTGGTAATCCGAACAGTACTCTTCCACATGTTTCTGAAGCCGAAGAAGGCAATATGGGGTATTATACACCTGTTCAAGGACTTGTTAATGAGATTGAACACAATGGAGAGTCTGGAGATAACAATGTAGAGACTGAGAGAAGCACGAAGCATGCGGCTCAACGGAACTACTACGATCATGTGGATATAGATATTGAGCAAATGGATAGTGATGATGATATTGAAGAATGGGGTAGACACTTAGAGGACTGTCATCAGCGAAGAATCGACATGGTAATCCAAACACTATGCAGTATGTTTGTGAAGCCGATGAAGACAATGATGGATGCCATTAGACTGATCACAACGAAGAGCGAGGAGACAGTGATGAATAGTGATATAGGCAGGGAGACTGATCACTTTGAAGAGTGCGAAGATAGTGATATAAGCAATGAGAGTGATCAGAGTGAAGAGCGTGGGGATAGTGACGATGATGGTATAAACAATGAGACTGATCTGTTCGAAGAGTGTGGAAACAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

417

Amino Acids

46.97

Weight (kDa)

4.6

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 104
AccI GTMKAC 2 cut(s) 688, 964
AciI CCGC 1 cut(s) 884
AclWI GGATC 4 cut(s) 221, 234, 658, 667
AcuI CTGAAG 2 cut(s) 364, 786
AfaI GTAC 3 cut(s) 269, 319, 748
AfiI CCNNNNNNNGG 2 cut(s) 104, 806
AflIII ACRYGT 2 cut(s) 270, 757
AgsI TTSAA 8 cut(s) 125, 220, 265, 451, 806, 827, 953, 1133
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 3 cut(s) 101, 471, 593
AluI AGCT 3 cut(s) 101, 471, 593
Alw26I GTCTC 6 cut(s) 15, 334, 854, 1082, 1112, 1211
AlwI GGATC 4 cut(s) 221, 234, 658, 667
AlwNI CAGNNNCTG 1 cut(s) 236
ApeKI GCWGC 1 cut(s) 33
AspLEI GCGC 1 cut(s) 398
AsuHPI GGTGA 2 cut(s) 236, 347
AsuII TTCGAA 1 cut(s) 1230
BaeI ACNNNNGTAYC 2 cut(s) 544, 577
BamHI GGATCC 1 cut(s) 226
BbsI GAAGAC 2 cut(s) 711, 1049
BbvI GCAGC 1 cut(s) 45
BccI CCATC 3 cut(s) 307, 1045, 1196
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 1 cut(s) 282
BciVI GTATCC 2 cut(s) 61, 572
BclI TGATCA 3 cut(s) 1068, 1123, 1168
BcoDI GTCTC 6 cut(s) 15, 334, 854, 1082, 1112, 1211
BfaI CTAG 2 cut(s) 141, 590
BfuI GTATCC 2 cut(s) 61, 572
BisI GCNGC 2 cut(s) 34, 885
BlsI GCNGC 2 cut(s) 35, 886
BmcAI AGTACT 1 cut(s) 748
Bme1390I CCNGG 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 1 cut(s) 1045
BpiI GAAGAC 2 cut(s) 711, 1049
BplI GAGNNNNNCTC 2 cut(s) 692, 724
BpmI CTGGAG 2 cut(s) 345, 864
Bpu14I TTCGAA 1 cut(s) 1230
BpuEI CTTGAG 3 cut(s) 101, 174, 579
BsaJI CCNNGG 1 cut(s) 281
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 342, 372
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 806
Bse1I ACTGG 1 cut(s) 328
Bse3DI GCAATG 2 cut(s) 28, 1165
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 3 cut(s) 153, 493, 1060
BseLI CCNNNNNNNGG 2 cut(s) 104, 806
BseMI GCAATG 2 cut(s) 28, 1165
BseMII CTCAG 1 cut(s) 855
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 3 cut(s) 166, 322, 1100
BseXI GCAGC 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 104, 806
BsmAI GTCTC 6 cut(s) 15, 334, 854, 1082, 1112, 1211
Bsp119I TTCGAA 1 cut(s) 1230
Bsp143I GATC 9 cut(s) 226, 610, 650, 659, 904, 1068, 1123, 1168, 1222
BspACI CCGC 1 cut(s) 884
BspCNI CTCAG 1 cut(s) 856
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 4 cut(s) 221, 234, 658, 667
BspQI GCTCTTC 2 cut(s) 1073, 1173
BspT104I TTCGAA 1 cut(s) 1230
BsrDI GCAATG 2 cut(s) 28, 1165
BsrI ACTGG 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 9 cut(s) 226, 610, 650, 659, 904, 1068, 1123, 1168, 1222
BssNAI GTATAC 1 cut(s) 689
Bst1107I GTATAC 1 cut(s) 689
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 7 cut(s) 238, 317, 557, 746, 980, 1093, 1247
Bst6I CTCTTC 8 cut(s) 184, 189, 715, 756, 1073, 1128, 1173, 1227
BstBI TTCGAA 1 cut(s) 1230
BstC8I GCNNGC 2 cut(s) 38, 882
BstDEI CTNAG 3 cut(s) 697, 864, 970
BstENI CCTNNNNNAGG 1 cut(s) 804
BstF5I GGATG 3 cut(s) 153, 493, 1060
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 9 cut(s) 229, 613, 653, 662, 907, 1071, 1126, 1171, 1225
BstMAI GTCTC 6 cut(s) 15, 334, 854, 1082, 1112, 1211
BstMBI GATC 9 cut(s) 226, 610, 650, 659, 904, 1068, 1123, 1168, 1222
BstMWI GCNNNNNNNGC 3 cut(s) 33, 107, 590
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 3 cut(s) 274, 761, 884
BstSCI CCNGG 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 2 cut(s) 711, 1049
BstX2I RGATCY 3 cut(s) 226, 650, 659
BstYI RGATCY 3 cut(s) 226, 650, 659
BstZ17I GTATAC 1 cut(s) 689
BsuI GTATCC 2 cut(s) 61, 572
BtsCI GGATG 3 cut(s) 153, 493, 1060
BtsIMutI CAGTG 2 cut(s) 1098, 1252
Cac8I GCNNGC 2 cut(s) 38, 882
CaiI CAGNNNCTG 1 cut(s) 236
CfoI GCGC 1 cut(s) 398
CseI GACGC 1 cut(s) 95
Csp6I GTAC 3 cut(s) 268, 318, 747
CviAII CATG 8 cut(s) 271, 419, 476, 614, 758, 881, 908, 1000
CviQI GTAC 3 cut(s) 268, 318, 747
DdeI CTNAG 3 cut(s) 697, 864, 970
DpnI GATC 9 cut(s) 228, 612, 652, 661, 906, 1070, 1125, 1170, 1224
DpnII GATC 9 cut(s) 226, 610, 650, 659, 904, 1068, 1123, 1168, 1222
Eam1104I CTCTTC 8 cut(s) 184, 189, 715, 756, 1073, 1128, 1173, 1227
EarI CTCTTC 8 cut(s) 184, 189, 715, 756, 1073, 1128, 1173, 1227
Eco57I CTGAAG 2 cut(s) 364, 786
EcoNI CCTNNNNNAGG 1 cut(s) 804
EcoRII CCWGG 1 cut(s) 280
FaeI CATG 8 cut(s) 274, 422, 479, 617, 761, 884, 911, 1003
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FatI CATG 8 cut(s) 270, 418, 475, 613, 757, 880, 907, 999
FbaI TGATCA 3 cut(s) 1068, 1123, 1168
FblI GTMKAC 2 cut(s) 688, 964
Fnu4HI GCNGC 2 cut(s) 34, 885
FokI GGATG 3 cut(s) 160, 500, 1067
Fsp4HI GCNGC 2 cut(s) 34, 885
FspBI CTAG 2 cut(s) 141, 590
GlaI GCGC 1 cut(s) 397
GluI GCNGC 2 cut(s) 34, 885
GsuI CTGGAG 2 cut(s) 345, 864
HgaI GACGC 1 cut(s) 95
HhaI GCGC 1 cut(s) 398
Hin1II CATG 8 cut(s) 274, 422, 479, 617, 761, 884, 911, 1003
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HincII GTYRAC 1 cut(s) 543
HindII GTYRAC 1 cut(s) 543
HinfI GANTC 7 cut(s) 251, 327, 641, 693, 707, 839, 993
HpaI GTTAAC 1 cut(s) 543
HphI GGTGA 2 cut(s) 236, 347
Hpy166II GTNNAC 3 cut(s) 543, 689, 965
Hpy188I TCNGA 8 cut(s) 339, 383, 487, 525, 670, 741, 766, 1173
Hpy188III TCNNGA 4 cut(s) 80, 230, 514, 843
Hpy8I GTNNAC 3 cut(s) 543, 689, 965
HpyAV CCTTC 4 cut(s) 251, 520, 569, 770
HpyCH4III ACNGT 7 cut(s) 238, 317, 557, 746, 980, 1093, 1247
HpyCH4IV ACGT 2 cut(s) 498, 665
HpyCH4V TGCA 2 cut(s) 33, 1020
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 107, 590
HpyF3I CTNAG 3 cut(s) 697, 864, 970
HpySE526I ACGT 2 cut(s) 498, 665
Hsp92II CATG 8 cut(s) 274, 422, 479, 617, 761, 884, 911, 1003
HspAI GCGC 1 cut(s) 396
Ksp22I TGATCA 3 cut(s) 1068, 1123, 1168
KspAI GTTAAC 1 cut(s) 543
Kzo9I GATC 9 cut(s) 226, 610, 650, 659, 904, 1068, 1123, 1168, 1222
LguI GCTCTTC 2 cut(s) 1073, 1173
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 1 cut(s) 1045
MaeI CTAG 2 cut(s) 141, 590
MaeII ACGT 2 cut(s) 498, 665
MaeIII GTNAC 2 cut(s) 403, 1193
MalI GATC 9 cut(s) 228, 612, 652, 661, 906, 1070, 1125, 1170, 1224
MboI GATC 9 cut(s) 226, 610, 650, 659, 904, 1068, 1123, 1168, 1222
MflI RGATCY 3 cut(s) 226, 650, 659
MluCI AATT 3 cut(s) 125, 255, 671
MlyI GAGTC 4 cut(s) 336, 687, 701, 848
MseI TTAA 5 cut(s) 113, 414, 542, 674, 816
MspR9I CCNGG 1 cut(s) 282
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 3 cut(s) 33, 107, 590
NdeII GATC 9 cut(s) 226, 610, 650, 659, 904, 1068, 1123, 1168, 1222
NlaIII CATG 8 cut(s) 274, 422, 479, 617, 761, 884, 911, 1003
NlaIV GGNNCC 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 1193
NspI RCATGY 3 cut(s) 274, 761, 884
NspV TTCGAA 1 cut(s) 1230
PaeI GCATGC 1 cut(s) 884
PciI ACATGT 2 cut(s) 270, 757
PciSI GCTCTTC 2 cut(s) 1073, 1173
PfeI GAWTC 3 cut(s) 251, 641, 993
PflMI CCANNNNNTGG 1 cut(s) 104
PkrI GCNGC 2 cut(s) 35, 886
PleI GAGTC 4 cut(s) 335, 687, 701, 847
PpsI GAGTC 4 cut(s) 335, 687, 701, 847
PscI ACATGT 2 cut(s) 270, 757
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 228
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 3 cut(s) 226, 650, 659
RsaI GTAC 3 cut(s) 269, 319, 748
RsaNI GTAC 3 cut(s) 268, 318, 747
SapI GCTCTTC 2 cut(s) 1073, 1173
SaqAI TTAA 5 cut(s) 113, 414, 542, 674, 816
SatI GCNGC 2 cut(s) 34, 885
Sau3AI GATC 9 cut(s) 226, 610, 650, 659, 904, 1068, 1123, 1168, 1222
ScaI AGTACT 1 cut(s) 748
SchI GAGTC 4 cut(s) 336, 687, 701, 848
ScrFI CCNGG 1 cut(s) 282
SetI ASST 9 cut(s) 103, 360, 438, 473, 501, 580, 595, 668, 802
SfaNI GCATC 1 cut(s) 1045
SfuI TTCGAA 1 cut(s) 1230
SmlI CTYRAG 3 cut(s) 80, 189, 594
SmoI CTYRAG 3 cut(s) 80, 189, 594
SphI GCATGC 1 cut(s) 884
Sse9I AATT 3 cut(s) 125, 255, 671
SsiI CCGC 1 cut(s) 884
SspI AATATT 2 cut(s) 163, 628
SspMI CTAG 2 cut(s) 141, 590
StyD4I CCNGG 1 cut(s) 280
TaaI ACNGT 7 cut(s) 238, 317, 557, 746, 980, 1093, 1247
TaiI ACGT 2 cut(s) 501, 668
TaqI TCGA 4 cut(s) 118, 195, 996, 1230
TasI AATT 3 cut(s) 125, 255, 671
TatI WGTACW 2 cut(s) 267, 746
TauI GCSGC 1 cut(s) 887
TfiI GAWTC 3 cut(s) 251, 641, 993
Tru1I TTAA 5 cut(s) 113, 414, 542, 674, 816
Tru9I TTAA 5 cut(s) 113, 414, 542, 674, 816
TscAI CASTG 2 cut(s) 1098, 1252
TseFI GTSAC 1 cut(s) 1193
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 1193
TspDTI ATGAA 6 cut(s) 164, 464, 620, 702, 1055, 1112
TspGWI ACGGA 1 cut(s) 907
TspRI CASTG 2 cut(s) 1098, 1252
Van91I CCANNNNNTGG 1 cut(s) 104
XagI CCTNNNNNAGG 1 cut(s) 804
XceI RCATGY 3 cut(s) 274, 761, 884
XmiI GTMKAC 2 cut(s) 688, 964
XspI CTAG 2 cut(s) 141, 590
ZrmI AGTACT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.