AT1G32470

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012278
Physical Location & Seq
Reverse (-)
11739304 .. 11740399
1096 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32470.1

Sequence Viewer

Length: 501 bp
ATGGCACTGAGAATGTGGGCTTCCTCTACAGCAAATGCTCTCAAGCTCTCTTCTTCTGCCTCCAAATCTCATCTCTTGCCAGCTTTCTCCATCTCCAGATGCTTCTCCTCAGTGTTGGAAGGACTTAAGTATGCAAATTCACATGAGTGGGTGAAACATGAAGGCTCAGTTGCTACAATTGGTATCACTGACCATGCGCAGGACCATTTAGGAGAAGTTGTGTTTGTGGAGCTGCCTGAAGCAAACAGTTCAGTGAGCAAAGAAAAAAGCTTTGGAGCTGTTGAGAGTGTTAAGGCCACAAGTGAGATTCTATCTCCAATCTCTGGTGAAGTCATTGAGGTTAACACAAAGCTCACCGAGTCACCTGGTTTGATCAACTCAAGCCCTTATGAAGATGGTTGGATGATAAAGGTGAAACCGAGCAGCCCCGCGGAGTTGGAAGCTCTGATGGGTCCAAAGGAATACACCAAGTTCTGCGAAGAAGAAGACGCTGCTCACTAG

Protein Analysis

166

Amino Acids

17.9

Weight (kDa)

5.11

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 42 - 161 2.2e-50 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32470 AT2G35370
fragaria_vesca FvH4_5g21610 FvH4_5g21610
malus_domestica MD06G1031000.v1.1 MD16G1277700.v1.1
prunus_persica Prupe.5G041300_v2.0.a1
pyrus_communis pycom06g02580 pycom16g24690
rosa_chinensis RchiOBHm_Chr7g0209071
rosa_laevigata RLG00000003170
rosa_multiflora Rmu_co8473347.1_g000001
rosa_roxburghii Rroxscaffold_3G00249780
rosa_rugosa Rorug07G0108700
rosa_samantha Rh7AG243200 Rh7BG238200 Rh7CG258400 Rh7DG250200
rosa_wichuraiana Rw7G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 198
AccB7I CCANNNNNTGG 1 cut(s) 323
AccII CGCG 1 cut(s) 431
AciI CCGC 2 cut(s) 429, 431
AcsI RAATTY 1 cut(s) 136
AcuI CTGAAG 1 cut(s) 258
AfiI CCNNNNNNNGG 2 cut(s) 199, 323
AflII CTTAAG 1 cut(s) 125
AjnI CCWGG 1 cut(s) 364
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AleI CACNNNNGTG 1 cut(s) 145
AluBI AGCT 7 cut(s) 46, 83, 232, 270, 278, 352, 443
AluI AGCT 7 cut(s) 46, 83, 232, 270, 278, 352, 443
AoxI GGCC 1 cut(s) 294
ApeKI GCWGC 3 cut(s) 232, 423, 491
ApoI RAATTY 1 cut(s) 136
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 2 cut(s) 202, 452
AsuHPI GGTGA 5 cut(s) 163, 338, 346, 354, 424
AvaII GGWCC 2 cut(s) 202, 452
BbsI GAAGAC 1 cut(s) 492
BbvI GCAGC 3 cut(s) 219, 435, 478
BccI CCATC 3 cut(s) 98, 389, 442
BciT130I CCWGG 1 cut(s) 366
BclI TGATCA 1 cut(s) 372
BfaI CTAG 1 cut(s) 499
BfmI CTRYAG 1 cut(s) 27
BfrI CTTAAG 1 cut(s) 125
BisI GCNGC 3 cut(s) 233, 424, 492
BlsI GCNGC 3 cut(s) 234, 425, 493
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 2 cut(s) 202, 452
BmgT120I GGNCC 2 cut(s) 202, 452
BmiI GGNNCC 1 cut(s) 453
BmrFI CCNGG 1 cut(s) 366
BmsI GCATC 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 492
BpmI CTGGAG 1 cut(s) 79
BpuEI CTTGAG 2 cut(s) 26, 364
BsaJI CCNNGG 1 cut(s) 429
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 323
BseBI CCWGG 1 cut(s) 366
BseDI CCNNGG 1 cut(s) 429
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 199, 323
BseMII CTCAG 2 cut(s) 123, 180
BseRI GAGGAG 1 cut(s) 97
BseXI GCAGC 3 cut(s) 219, 435, 478
Bsh1236I CGCG 1 cut(s) 431
BshFI GGCC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 199, 323
BsnI GGCC 1 cut(s) 296
Bsp143I GATC 1 cut(s) 372
BspACI CCGC 2 cut(s) 429, 431
BspANI GGCC 1 cut(s) 296
BspCNI CTCAG 2 cut(s) 122, 179
BspFNI CGCG 1 cut(s) 431
BspLI GGNNCC 1 cut(s) 453
BspTI CTTAAG 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 1 cut(s) 55
BstAFI CTTAAG 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 3 cut(s) 8, 109, 166
BstDSI CCRYGG 1 cut(s) 429
BstF5I GGATG 1 cut(s) 408
BstFNI CGCG 1 cut(s) 431
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 1 cut(s) 375
BstMBI GATC 1 cut(s) 372
BstNI CCWGG 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 364
BstSFI CTRYAG 1 cut(s) 27
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 3 cut(s) 219, 435, 478
BstV2I GAAGAC 1 cut(s) 492
BsuRI GGCC 1 cut(s) 296
BtgI CCRYGG 1 cut(s) 429
BtsCI GGATG 1 cut(s) 408
BtsIMutI CAGTG 4 cut(s) 5, 117, 186, 258
Cac8I GCNNGC 1 cut(s) 81
CfoI GCGC 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 202, 452
Cfr42I CCGCGG 1 cut(s) 432
CsiI ACCWGGT 1 cut(s) 364
CviAII CATG 3 cut(s) 143, 158, 194
DdeI CTNAG 3 cut(s) 8, 109, 166
DpnI GATC 1 cut(s) 374
DpnII GATC 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 202, 452
Eco57I CTGAAG 1 cut(s) 258
EcoRII CCWGG 1 cut(s) 364
FaeI CATG 3 cut(s) 146, 161, 197
FaiI YATR 5 cut(s) 132, 144, 159, 195, 390
FatI CATG 3 cut(s) 142, 157, 193
FauI CCCGC 1 cut(s) 436
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 233, 424, 492
FokI GGATG 1 cut(s) 415
Fsp4HI GCNGC 3 cut(s) 233, 424, 492
FspBI CTAG 1 cut(s) 499
FspI TGCGCA 1 cut(s) 198
GlaI GCGC 1 cut(s) 198
GluI GCNGC 3 cut(s) 233, 424, 492
GsuI CTGGAG 1 cut(s) 79
HaeIII GGCC 1 cut(s) 296
HhaI GCGC 1 cut(s) 199
Hin1II CATG 3 cut(s) 146, 161, 197
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 268
HinfI GANTC 2 cut(s) 307, 359
HpaI GTTAAC 1 cut(s) 343
HphI GGTGA 5 cut(s) 163, 338, 346, 354, 424
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 1 cut(s) 96
Hpy8I GTNNAC 1 cut(s) 343
HpyAV CCTTC 2 cut(s) 113, 155
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4V TGCA 1 cut(s) 134
HpyF3I CTNAG 3 cut(s) 8, 109, 166
Hsp92II CATG 3 cut(s) 146, 161, 197
HspAI GCGC 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 372
KspAI GTTAAC 1 cut(s) 343
KspI CCGCGG 1 cut(s) 432
Kzo9I GATC 1 cut(s) 372
LmnI GCTCC 2 cut(s) 229, 275
LpnPI CCDG 7 cut(s) 93, 109, 185, 249, 309, 351, 378
Lsp1109I GCAGC 3 cut(s) 219, 435, 478
LweI GCATC 1 cut(s) 89
MabI ACCWGGT 1 cut(s) 364
MaeI CTAG 1 cut(s) 499
MaeIII GTNAC 1 cut(s) 360
MalI GATC 1 cut(s) 374
MboI GATC 1 cut(s) 372
MboII GAAGA 6 cut(s) 42, 45, 404, 491, 494, 497
MfeI CAATTG 1 cut(s) 177
MluCI AATT 2 cut(s) 136, 177
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 3 cut(s) 96, 380, 417
MnlI CCTC 4 cut(s) 34, 70, 118, 331
MseI TTAA 3 cut(s) 126, 291, 342
MslI CAYNNNNRTG 1 cut(s) 145
MspA1I CMGCKG 1 cut(s) 431
MspCI CTTAAG 1 cut(s) 125
MspR9I CCNGG 1 cut(s) 366
MunI CAATTG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 366
MvnI CGCG 1 cut(s) 431
NdeII GATC 1 cut(s) 372
NlaIII CATG 3 cut(s) 146, 161, 197
NlaIV GGNNCC 1 cut(s) 453
NmuCI GTSAC 1 cut(s) 360
NsbI TGCGCA 1 cut(s) 198
OliI CACNNNNGTG 1 cut(s) 145
PfeI GAWTC 1 cut(s) 307
PflMI CCANNNNNTGG 1 cut(s) 323
PkrI GCNGC 3 cut(s) 234, 425, 493
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp6I CCWGG 1 cut(s) 364
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 1 cut(s) 453
PspPI GGNCC 2 cut(s) 202, 452
RseI CAYNNNNRTG 1 cut(s) 145
SacII CCGCGG 1 cut(s) 432
SaqAI TTAA 3 cut(s) 126, 291, 342
SatI GCNGC 3 cut(s) 233, 424, 492
Sau3AI GATC 1 cut(s) 372
Sau96I GGNCC 2 cut(s) 202, 452
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 1 cut(s) 366
SexAI ACCWGGT 1 cut(s) 364
SfaNI GCATC 1 cut(s) 89
SfcI CTRYAG 1 cut(s) 27
Sfr303I CCGCGG 1 cut(s) 432
SgrBI CCGCGG 1 cut(s) 432
SinI GGWCC 2 cut(s) 202, 452
SmiMI CAYNNNNRTG 1 cut(s) 145
SmlI CTYRAG 3 cut(s) 41, 125, 379
SmoI CTYRAG 3 cut(s) 41, 125, 379
Sse9I AATT 2 cut(s) 136, 177
SsiI CCGC 2 cut(s) 429, 431
SspMI CTAG 1 cut(s) 499
StyD4I CCNGG 1 cut(s) 364
TaaI ACNGT 1 cut(s) 248
TasI AATT 2 cut(s) 136, 177
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 3 cut(s) 126, 291, 342
Tru9I TTAA 3 cut(s) 126, 291, 342
TscAI CASTG 4 cut(s) 12, 117, 193, 258
TseFI GTSAC 1 cut(s) 360
TseI GCWGC 3 cut(s) 232, 423, 491
Tsp45I GTSAC 1 cut(s) 360
TspDTI ATGAA 2 cut(s) 174, 405
TspRI CASTG 4 cut(s) 12, 117, 193, 258
Van91I CCANNNNNTGG 1 cut(s) 323
Vha464I CTTAAG 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 202, 452
XapI RAATTY 1 cut(s) 136
XspI CTAG 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.