RchiOBHm_Chr7g0209071

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012278
Physical Location & Seq
Reverse (-)
26451141 .. 26452809
1669 bp
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UTR
Exon/CDS
Intron
PRQ18712

Sequence Viewer

Length: 498 bp
ATGGCAATGAAAATGTGGGCTTCTTCTACAGCAAATGCACTCAGAATCTCTGGTGCCTCCAAAGCTCAACTCTCCCCTGCATTTTCTCTCTCCAGATGCTTTTCCACTGTTCTGGATGGGTTGAAGTATGCATCTTCACATGAATGGGTGAAGCATGAAGGCTCAGTTGCTACAATTGGTATCACTGACCATGCTCAGGACCATCTTGGTGAAGTGGTGTTTGTGGAGCTGCCAGAACCCGGTACAGCAGTCTCCCAAAGCAGCAGCTTCGGAAATGTTGAAAGTGTGAAAGCAACCAGTGATGTCAATTCCCCAATCTCTGGTGAGGTTGTTGAGGTTAACCCGAAGCTGAGTGAAACACCTGGTTTGATCAATACAAGCCCATATGAAGATGGATGGATGATCAAGGTGAAGCCCAGCAATGCATCAGAACTAGAATCCTTGTTGGGTCCTAAAGAATACACGAAATTCTGCGAGGAAGAAGATGCAGCCCACTAG

Protein Analysis

165

Amino Acids

17.63

Weight (kDa)

4.86

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 41 - 160 1.6e-49 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32470 AT2G35370
fragaria_vesca FvH4_5g21610 FvH4_5g21610
malus_domestica MD06G1031000.v1.1 MD16G1277700.v1.1
prunus_persica Prupe.5G041300_v2.0.a1
pyrus_communis pycom06g02580 pycom16g24690
rosa_chinensis RchiOBHm_Chr7g0209071
rosa_laevigata RLG00000003170
rosa_multiflora Rmu_co8473347.1_g000001
rosa_roxburghii Rroxscaffold_3G00249780
rosa_rugosa Rorug07G0108700
rosa_samantha Rh7AG243200 Rh7BG238200 Rh7CG258400 Rh7DG250200
rosa_wichuraiana Rw7G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 53
AccB7I CCANNNNNTGG 1 cut(s) 320
AcsI RAATTY 1 cut(s) 467
AfaI GTAC 1 cut(s) 244
AfiI CCNNNNNNNGG 3 cut(s) 196, 239, 320
AgsI TTSAA 2 cut(s) 124, 281
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 4 cut(s) 65, 229, 267, 349
AluI AGCT 4 cut(s) 65, 229, 267, 349
Alw26I GTCTC 1 cut(s) 256
ApeKI GCWGC 4 cut(s) 229, 261, 264, 488
ApoI RAATTY 1 cut(s) 467
AspS9I GGNCC 2 cut(s) 199, 449
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 4 cut(s) 160, 221, 335, 421
AvaII GGWCC 2 cut(s) 199, 449
BaeI ACNNNNGTAYC 2 cut(s) 234, 267
BanI GGYRCC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 216, 273, 276
BccI CCATC 4 cut(s) 110, 210, 386, 390
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 1 cut(s) 363
BclI TGATCA 2 cut(s) 369, 402
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 256
BfaI CTAG 2 cut(s) 434, 496
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 4 cut(s) 230, 262, 265, 489
BlsI GCNGC 4 cut(s) 231, 263, 266, 490
Bme1390I CCNGG 2 cut(s) 240, 363
Bme18I GGWCC 2 cut(s) 199, 449
BmgT120I GGNCC 2 cut(s) 199, 449
BmiI GGNNCC 2 cut(s) 55, 450
BmrFI CCNGG 2 cut(s) 240, 363
BmsI GCATC 4 cut(s) 86, 140, 434, 475
BpmI CTGGAG 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 239, 320
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 2 cut(s) 12, 427
BseBI CCWGG 1 cut(s) 363
BseGI GGATG 3 cut(s) 121, 401, 405
BseLI CCNNNNNNNGG 3 cut(s) 196, 239, 320
BseMI GCAATG 2 cut(s) 12, 427
BseMII CTCAG 4 cut(s) 55, 177, 209, 341
BseNI ACTGG 1 cut(s) 297
BseXI GCAGC 3 cut(s) 216, 273, 276
BseYI CCCAGC 1 cut(s) 416
BshNI GGYRCC 1 cut(s) 53
BsiSI CCGG 1 cut(s) 240
BslI CCNNNNNNNGG 3 cut(s) 196, 239, 320
BsmAI GTCTC 1 cut(s) 256
Bsp143I GATC 2 cut(s) 369, 402
BspCNI CTCAG 4 cut(s) 54, 176, 208, 342
BspLI GGNNCC 2 cut(s) 55, 450
BspT107I GGYRCC 1 cut(s) 53
BsrDI GCAATG 2 cut(s) 12, 427
BsrI ACTGG 1 cut(s) 297
BssMI GATC 2 cut(s) 369, 402
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 109
BstDEI CTNAG 4 cut(s) 41, 163, 195, 350
BstF5I GGATG 3 cut(s) 121, 401, 405
BstKTI GATC 2 cut(s) 372, 405
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 2 cut(s) 369, 402
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 2 cut(s) 238, 361
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 3 cut(s) 216, 273, 276
BstXI CCANNNNNNTGG 1 cut(s) 112
BtsCI GGATG 3 cut(s) 121, 401, 405
BtsIMutI CAGTG 3 cut(s) 105, 183, 304
Cfr13I GGNCC 2 cut(s) 199, 449
CsiI ACCWGGT 1 cut(s) 361
Csp6I GTAC 1 cut(s) 243
CviAII CATG 3 cut(s) 140, 155, 191
CviJI RGCY 9 cut(s) 20, 65, 162, 229, 267, 349, 381, 415, 491
CviKI_1 RGCY 9 cut(s) 20, 65, 162, 229, 267, 349, 381, 415, 491
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 4 cut(s) 41, 163, 195, 350
DpnI GATC 2 cut(s) 371, 404
DpnII GATC 2 cut(s) 369, 402
Eco47I GGWCC 2 cut(s) 199, 449
EcoO109I RGGNCCY 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 361
EcoT22I ATGCAT 2 cut(s) 133, 427
FaeI CATG 3 cut(s) 143, 158, 194
FaiI YATR 6 cut(s) 129, 141, 156, 192, 385, 387
FatI CATG 3 cut(s) 139, 154, 190
FauNDI CATATG 1 cut(s) 385
FbaI TGATCA 2 cut(s) 369, 402
Fnu4HI GCNGC 4 cut(s) 230, 262, 265, 489
FokI GGATG 3 cut(s) 128, 408, 412
Fsp4HI GCNGC 4 cut(s) 230, 262, 265, 489
FspBI CTAG 2 cut(s) 434, 496
GluI GCNGC 4 cut(s) 230, 262, 265, 489
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 76
HapII CCGG 1 cut(s) 240
Hin1II CATG 3 cut(s) 143, 158, 194
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 2 cut(s) 45, 437
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 1 cut(s) 240
HphI GGTGA 4 cut(s) 160, 221, 335, 421
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 3 cut(s) 44, 272, 430
Hpy188III TCNNGA 3 cut(s) 93, 113, 197
Hpy8I GTNNAC 1 cut(s) 340
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 5 cut(s) 38, 80, 131, 425, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 4 cut(s) 41, 163, 195, 350
Hsp92II CATG 3 cut(s) 143, 158, 194
Ksp22I TGATCA 2 cut(s) 369, 402
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 2 cut(s) 369, 402
LmnI GCTCC 1 cut(s) 226
Lsp1109I GCAGC 3 cut(s) 216, 273, 276
LweI GCATC 4 cut(s) 86, 140, 434, 475
MabI ACCWGGT 1 cut(s) 361
MaeI CTAG 2 cut(s) 434, 496
MalI GATC 2 cut(s) 371, 404
MboI GATC 2 cut(s) 369, 402
MboII GAAGA 5 cut(s) 15, 126, 401, 491, 494
MfeI CAATTG 1 cut(s) 174
MluCI AATT 3 cut(s) 174, 307, 467
MnlI CCTC 4 cut(s) 67, 319, 328, 469
Mph1103I ATGCAT 2 cut(s) 133, 427
MseI TTAA 1 cut(s) 339
MslI CAYNNNNRTG 2 cut(s) 142, 207
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 240, 363
MunI CAATTG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 363
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 385
NdeII GATC 2 cut(s) 369, 402
NlaIII CATG 3 cut(s) 143, 158, 194
NlaIV GGNNCC 2 cut(s) 55, 450
NsiI ATGCAT 2 cut(s) 133, 427
PfeI GAWTC 2 cut(s) 45, 437
PflMI CCANNNNNTGG 1 cut(s) 320
PkrI GCNGC 4 cut(s) 231, 263, 266, 490
PpuMI RGGWCCY 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 2 cut(s) 55, 450
PspPI GGNCC 2 cut(s) 199, 449
PspPPI RGGWCCY 1 cut(s) 449
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 2 cut(s) 142, 207
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 4 cut(s) 230, 262, 265, 489
Sau3AI GATC 2 cut(s) 369, 402
Sau96I GGNCC 2 cut(s) 199, 449
ScrFI CCNGG 2 cut(s) 240, 363
SetI ASST 8 cut(s) 67, 231, 269, 330, 339, 351, 364, 411
SexAI ACCWGGT 1 cut(s) 361
SfaNI GCATC 4 cut(s) 86, 140, 434, 475
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 2 cut(s) 199, 449
SmiMI CAYNNNNRTG 2 cut(s) 142, 207
Sse9I AATT 3 cut(s) 174, 307, 467
SspMI CTAG 2 cut(s) 434, 496
StyD4I CCNGG 2 cut(s) 238, 361
TaaI ACNGT 1 cut(s) 109
TasI AATT 3 cut(s) 174, 307, 467
TfiI GAWTC 2 cut(s) 45, 437
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 3 cut(s) 112, 190, 304
TseI GCWGC 4 cut(s) 229, 261, 264, 488
TspDTI ATGAA 4 cut(s) 23, 156, 171, 402
TspRI CASTG 3 cut(s) 112, 190, 304
Van91I CCANNNNNTGG 1 cut(s) 320
VpaK11BI GGWCC 2 cut(s) 199, 449
XapI RAATTY 1 cut(s) 467
XspI CTAG 2 cut(s) 434, 496
Zsp2I ATGCAT 2 cut(s) 133, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.