Rh7DG250200

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012278
Physical Location & Seq
Reverse (-)
25930595 .. 25932310
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG250200.1

Sequence Viewer

Length: 387 bp
ATGGCAATGAAAATGTGGGCTTCTTCTACAGCAAATGCACTCAGAATCTCTGGTGCCTCCAAAGCTCAACTCTCCCCTGCATTTTCTCTCTCCAGATGCTTTTCCACTGTTCTGGATGGGTTGAAGTATGCATCTTCACATGAATGGGTGAAGCATGAAGGCTCAGTTGCTACAATTGGTATCACTGACCATGCTCAGGACCATCTTGGTGAAGTGGTGTTTGTGGAGCTGCCAGAACCCGGTACAGCAGTCTCCCAAAGCAGCAGCTTCGGAAATGTTGAAAGTGTGAAAGCAACCAGTGATGTCAATTCCCCAATCTCTGGTGAGGTTGTTGAGGTTAACCCGAAGCTGAGTGAAACACCCGGTTTGGTAATTGCTTTTCCTTAA

Protein Analysis

128

Amino Acids

13.42

Weight (kDa)

5.21

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 41 - 124 7.2e-33 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32470 AT2G35370
fragaria_vesca FvH4_5g21610 FvH4_5g21610
malus_domestica MD06G1031000.v1.1 MD16G1277700.v1.1
prunus_persica Prupe.5G041300_v2.0.a1
pyrus_communis pycom06g02580 pycom16g24690
rosa_chinensis RchiOBHm_Chr7g0209071
rosa_laevigata RLG00000003170
rosa_multiflora Rmu_co8473347.1_g000001
rosa_roxburghii Rroxscaffold_3G00249780
rosa_rugosa Rorug07G0108700
rosa_samantha Rh7AG243200 Rh7BG238200 Rh7CG258400 Rh7DG250200
rosa_wichuraiana Rw7G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 53
AccB7I CCANNNNNTGG 1 cut(s) 320
AfaI GTAC 1 cut(s) 244
AfiI CCNNNNNNNGG 3 cut(s) 196, 239, 320
AgsI TTSAA 2 cut(s) 124, 281
AluBI AGCT 4 cut(s) 65, 229, 267, 349
AluI AGCT 4 cut(s) 65, 229, 267, 349
Alw26I GTCTC 1 cut(s) 256
ApeKI GCWGC 3 cut(s) 229, 261, 264
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 2 cut(s) 240, 363
AsuHPI GGTGA 3 cut(s) 160, 221, 335
AvaII GGWCC 1 cut(s) 199
BaeI ACNNNNGTAYC 2 cut(s) 234, 267
BanI GGYRCC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 216, 273, 276
BccI CCATC 2 cut(s) 110, 210
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BcnI CCSGG 2 cut(s) 240, 363
BcoDI GTCTC 1 cut(s) 256
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 3 cut(s) 230, 262, 265
BlsI GCNGC 3 cut(s) 231, 263, 266
Bme1390I CCNGG 2 cut(s) 240, 363
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 240, 363
BmsI GCATC 2 cut(s) 86, 140
BpmI CTGGAG 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 195
BpuMI CCSGG 2 cut(s) 240, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 239, 320
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 1 cut(s) 12
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 3 cut(s) 196, 239, 320
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 4 cut(s) 55, 177, 209, 341
BseNI ACTGG 1 cut(s) 297
BseXI GCAGC 3 cut(s) 216, 273, 276
BshNI GGYRCC 1 cut(s) 53
BsiSI CCGG 2 cut(s) 240, 363
BslI CCNNNNNNNGG 3 cut(s) 196, 239, 320
BsmAI GTCTC 1 cut(s) 256
BspCNI CTCAG 4 cut(s) 54, 176, 208, 342
BspLI GGNNCC 1 cut(s) 55
BspT107I GGYRCC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 109
BstDEI CTNAG 4 cut(s) 41, 163, 195, 350
BstF5I GGATG 1 cut(s) 121
BstMAI GTCTC 1 cut(s) 256
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstSCI CCNGG 2 cut(s) 238, 361
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 3 cut(s) 216, 273, 276
BstXI CCANNNNNNTGG 1 cut(s) 112
BtsCI GGATG 1 cut(s) 121
BtsIMutI CAGTG 3 cut(s) 105, 183, 304
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 1 cut(s) 243
CviAII CATG 3 cut(s) 140, 155, 191
CviJI RGCY 6 cut(s) 20, 65, 162, 229, 267, 349
CviKI_1 RGCY 6 cut(s) 20, 65, 162, 229, 267, 349
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 4 cut(s) 41, 163, 195, 350
Eco47I GGWCC 1 cut(s) 199
EcoT22I ATGCAT 1 cut(s) 133
FaeI CATG 3 cut(s) 143, 158, 194
FaiI YATR 4 cut(s) 129, 141, 156, 192
FatI CATG 3 cut(s) 139, 154, 190
Fnu4HI GCNGC 3 cut(s) 230, 262, 265
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 3 cut(s) 230, 262, 265
GluI GCNGC 3 cut(s) 230, 262, 265
GsuI CTGGAG 1 cut(s) 76
HapII CCGG 2 cut(s) 240, 363
Hin1II CATG 3 cut(s) 143, 158, 194
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 1 cut(s) 45
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 2 cut(s) 240, 363
HphI GGTGA 3 cut(s) 160, 221, 335
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 44, 272
Hpy188III TCNNGA 3 cut(s) 93, 113, 197
Hpy8I GTNNAC 1 cut(s) 340
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 3 cut(s) 38, 80, 131
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 4 cut(s) 41, 163, 195, 350
Hsp92II CATG 3 cut(s) 143, 158, 194
KspAI GTTAAC 1 cut(s) 340
LmnI GCTCC 1 cut(s) 226
Lsp1109I GCAGC 3 cut(s) 216, 273, 276
LweI GCATC 2 cut(s) 86, 140
MboII GAAGA 2 cut(s) 15, 126
MfeI CAATTG 1 cut(s) 174
MluCI AATT 3 cut(s) 174, 307, 372
MnlI CCTC 3 cut(s) 67, 319, 328
Mph1103I ATGCAT 1 cut(s) 133
MseI TTAA 2 cut(s) 339, 385
MslI CAYNNNNRTG 2 cut(s) 142, 207
MspI CCGG 2 cut(s) 240, 363
MspR9I CCNGG 2 cut(s) 240, 363
MunI CAATTG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 2 cut(s) 240, 363
NlaIII CATG 3 cut(s) 143, 158, 194
NlaIV GGNNCC 1 cut(s) 55
NsiI ATGCAT 1 cut(s) 133
PfeI GAWTC 1 cut(s) 45
PflMI CCANNNNNTGG 1 cut(s) 320
PkrI GCNGC 3 cut(s) 231, 263, 266
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 1 cut(s) 199
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 2 cut(s) 142, 207
SaqAI TTAA 2 cut(s) 339, 385
SatI GCNGC 3 cut(s) 230, 262, 265
Sau96I GGNCC 1 cut(s) 199
ScrFI CCNGG 2 cut(s) 240, 363
SetI ASST 6 cut(s) 67, 231, 269, 330, 339, 351
SfaNI GCATC 2 cut(s) 86, 140
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 199
SmiMI CAYNNNNRTG 2 cut(s) 142, 207
Sse9I AATT 3 cut(s) 174, 307, 372
StyD4I CCNGG 2 cut(s) 238, 361
TaaI ACNGT 1 cut(s) 109
TasI AATT 3 cut(s) 174, 307, 372
TfiI GAWTC 1 cut(s) 45
Tru1I TTAA 2 cut(s) 339, 385
Tru9I TTAA 2 cut(s) 339, 385
TscAI CASTG 3 cut(s) 112, 190, 304
TseI GCWGC 3 cut(s) 229, 261, 264
TspDTI ATGAA 3 cut(s) 23, 156, 171
TspRI CASTG 3 cut(s) 112, 190, 304
Van91I CCANNNNNTGG 1 cut(s) 320
VpaK11BI GGWCC 1 cut(s) 199
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.