MD06G1031000.v1.1

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012278
Physical Location & Seq
Reverse (-)
3741604 .. 3743283
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1031000.v1.1.491

Sequence Viewer

Length: 498 bp
ATGGCACTGAGAATGTGGGCTTCTTCTACAGCCAATGCACTCAGAATCTCCACTGCCTCCAAATCCCACCTCGCTCCTGCATTTTCTATCTCCAGATGCTTTTCTACTGTTTTGGATGGATTGAAGTATGCATCTTCACATGAGTGGGTGAAGCATGAAGGCTCTGTGGCAACAATTGGCATCACTGATCATGCTCAGGACCATCTTGGAGAAGTAGTGTTTGTTGAGCTACCAGAAGCTGGTCGCTCAGTCTCTCAAGGAGGCAGCTTTGGAGCTGTGGAAAGTGTCAAAGCAACCAGTGATGTAAATTCCCCAATCTCCGGCGAAGTTGTCGAGGTTAACTCAAAGCTTACTGAATCACCTGGTTTGATCAATTCGAGCCCATATGAAGATGGATGGATGATCAAGATCAAGCCCAGTAGTCCATCAGAACTAGAATCCTTGTTGGGACCTAAAGAATACACGAAGTTTTGTGAGGAAGAAAATGCAGCCCATTGA

Protein Analysis

166

Amino Acids

17.62

Weight (kDa)

5.19

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 41 - 160 5.1e-50 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32470 AT2G35370
fragaria_vesca FvH4_5g21610 FvH4_5g21610
malus_domestica MD06G1031000.v1.1 MD16G1277700.v1.1
prunus_persica Prupe.5G041300_v2.0.a1
pyrus_communis pycom06g02580 pycom16g24690
rosa_chinensis RchiOBHm_Chr7g0209071
rosa_laevigata RLG00000003170
rosa_multiflora Rmu_co8473347.1_g000001
rosa_roxburghii Rroxscaffold_3G00249780
rosa_rugosa Rorug07G0108700
rosa_samantha Rh7AG243200 Rh7BG238200 Rh7CG258400 Rh7DG250200
rosa_wichuraiana Rw7G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 239
AcsI RAATTY 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 239, 320
AgsI TTSAA 1 cut(s) 124
AjnI CCWGG 1 cut(s) 361
AleI CACNNNNGTG 1 cut(s) 142
AluBI AGCT 5 cut(s) 229, 239, 267, 275, 349
AluI AGCT 5 cut(s) 229, 239, 267, 275, 349
Alw26I GTCTC 1 cut(s) 256
AlwNI CAGNNNCTG 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 264, 488
ApoI RAATTY 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 199, 449
AsuHPI GGTGA 2 cut(s) 160, 351
AvaII GGWCC 2 cut(s) 199, 449
BanII GRGCYC 1 cut(s) 383
BbvI GCAGC 1 cut(s) 276
BccI CCATC 5 cut(s) 110, 210, 386, 390, 433
BciT130I CCWGG 1 cut(s) 363
BclI TGATCA 3 cut(s) 187, 369, 402
BcoDI GTCTC 1 cut(s) 256
BfaI CTAG 1 cut(s) 434
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 2 cut(s) 265, 489
BlsI GCNGC 2 cut(s) 266, 490
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 2 cut(s) 199, 449
BmgT120I GGNCC 2 cut(s) 199, 449
BmiI GGNNCC 1 cut(s) 450
BmrFI CCNGG 1 cut(s) 363
BmrI ACTGGG 1 cut(s) 411
BmsI GCATC 3 cut(s) 86, 140, 189
BmuI ACTGGG 1 cut(s) 411
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 240
BsaBI GATNNNNATC 1 cut(s) 407
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 320
Bse1I ACTGG 2 cut(s) 297, 417
Bse8I GATNNNNATC 1 cut(s) 407
BseBI CCWGG 1 cut(s) 363
BseGI GGATG 3 cut(s) 121, 401, 405
BseJI GATNNNNATC 1 cut(s) 407
BseLI CCNNNNNNNGG 2 cut(s) 239, 320
BseMII CTCAG 3 cut(s) 55, 209, 261
BseNI ACTGG 2 cut(s) 297, 417
BseXI GCAGC 1 cut(s) 276
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 1 cut(s) 462
BslI CCNNNNNNNGG 2 cut(s) 239, 320
BsmAI GTCTC 1 cut(s) 256
BsmFI GGGAC 1 cut(s) 462
Bsp1286I GDGCHC 1 cut(s) 383
Bsp143I GATC 4 cut(s) 187, 369, 402, 408
BspCNI CTCAG 3 cut(s) 54, 208, 260
BspLI GGNNCC 1 cut(s) 450
BsrI ACTGG 2 cut(s) 297, 417
BssMI GATC 4 cut(s) 187, 369, 402, 408
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 109
BstDEI CTNAG 4 cut(s) 8, 41, 195, 247
BstF5I GGATG 3 cut(s) 121, 401, 405
BstKTI GATC 4 cut(s) 190, 372, 405, 411
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 4 cut(s) 187, 369, 402, 408
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 276
BtsCI GGATG 3 cut(s) 121, 401, 405
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 4 cut(s) 5, 51, 183, 304
CaiI CAGNNNCTG 1 cut(s) 239
Cfr13I GGNCC 2 cut(s) 199, 449
CsiI ACCWGGT 1 cut(s) 361
CviAII CATG 3 cut(s) 140, 155, 191
DdeI CTNAG 4 cut(s) 8, 41, 195, 247
DpnI GATC 4 cut(s) 189, 371, 404, 410
DpnII GATC 4 cut(s) 187, 369, 402, 408
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 2 cut(s) 199, 449
EcoO109I RGGNCCY 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 361
EcoT22I ATGCAT 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 383
FaeI CATG 3 cut(s) 143, 158, 194
FaiI YATR 6 cut(s) 129, 141, 156, 192, 385, 387
FaqI GGGAC 1 cut(s) 462
FatI CATG 3 cut(s) 139, 154, 190
FauNDI CATATG 1 cut(s) 385
FbaI TGATCA 3 cut(s) 187, 369, 402
Fnu4HI GCNGC 2 cut(s) 265, 489
FokI GGATG 3 cut(s) 128, 408, 412
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 2 cut(s) 265, 489
FspBI CTAG 1 cut(s) 434
GluI GCNGC 2 cut(s) 265, 489
GsuI CTGGAG 1 cut(s) 76
HapII CCGG 1 cut(s) 321
Hin1II CATG 3 cut(s) 143, 158, 194
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 3 cut(s) 45, 356, 437
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 1 cut(s) 321
HphI GGTGA 2 cut(s) 160, 351
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 44, 430
Hpy188III TCNNGA 3 cut(s) 93, 197, 406
Hpy8I GTNNAC 1 cut(s) 340
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 4 cut(s) 38, 80, 131, 488
HpyF3I CTNAG 4 cut(s) 8, 41, 195, 247
Hsp92II CATG 3 cut(s) 143, 158, 194
Ksp22I TGATCA 3 cut(s) 187, 369, 402
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 4 cut(s) 187, 369, 402, 408
LmnI GCTCC 2 cut(s) 79, 272
Lsp1109I GCAGC 1 cut(s) 276
LweI GCATC 3 cut(s) 86, 140, 189
MabI ACCWGGT 1 cut(s) 361
MaeI CTAG 1 cut(s) 434
MalI GATC 4 cut(s) 189, 371, 404, 410
MboI GATC 4 cut(s) 187, 369, 402, 408
MboII GAAGA 4 cut(s) 15, 126, 401, 491
MfeI CAATTG 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 3 cut(s) 174, 307, 373
MnlI CCTC 5 cut(s) 67, 80, 254, 328, 469
Mph1103I ATGCAT 1 cut(s) 133
MseI TTAA 1 cut(s) 339
MslI CAYNNNNRTG 1 cut(s) 142
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 363
MunI CAATTG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 363
NdeI CATATG 1 cut(s) 385
NdeII GATC 4 cut(s) 187, 369, 402, 408
NlaIII CATG 3 cut(s) 143, 158, 194
NlaIV GGNNCC 1 cut(s) 450
NsiI ATGCAT 1 cut(s) 133
OliI CACNNNNGTG 1 cut(s) 142
PfeI GAWTC 3 cut(s) 45, 356, 437
PflMI CCANNNNNTGG 1 cut(s) 239
PkrI GCNGC 2 cut(s) 266, 490
PpuMI RGGWCCY 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 450
PspPI GGNCC 2 cut(s) 199, 449
PspPPI RGGWCCY 1 cut(s) 449
PstNI CAGNNNCTG 1 cut(s) 239
RseI CAYNNNNRTG 1 cut(s) 142
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 2 cut(s) 265, 489
Sau3AI GATC 4 cut(s) 187, 369, 402, 408
Sau96I GGNCC 2 cut(s) 199, 449
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 1 cut(s) 383
SetI ASST 9 cut(s) 72, 231, 241, 269, 277, 339, 351, 364, 454
SexAI ACCWGGT 1 cut(s) 361
SfaNI GCATC 3 cut(s) 86, 140, 189
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 2 cut(s) 199, 449
SmiMI CAYNNNNRTG 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 3 cut(s) 174, 307, 373
SspMI CTAG 1 cut(s) 434
StyD4I CCNGG 1 cut(s) 361
TaaI ACNGT 1 cut(s) 109
TaqI TCGA 2 cut(s) 333, 377
TasI AATT 3 cut(s) 174, 307, 373
TfiI GAWTC 3 cut(s) 45, 356, 437
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 4 cut(s) 12, 58, 190, 304
TseI GCWGC 2 cut(s) 264, 488
TspDTI ATGAA 2 cut(s) 171, 402
TspRI CASTG 4 cut(s) 12, 58, 190, 304
Van91I CCANNNNNTGG 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 199, 449
XapI RAATTY 1 cut(s) 307
XspI CTAG 1 cut(s) 434
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.