AT2G35370

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012278
Physical Location & Seq
Forward (+)
14891041 .. 14892298
1258 bp
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UTR
Exon/CDS
Intron
AT2G35370.1

Sequence Viewer

Length: 498 bp
ATGGCACTAAGAATGTGGGCTTCTTCTACAGCAAACGCTCTCAAGCTTTCTTCTTCTGTTTCCAAGTCTCATCTCTCTCCTTTCTCCTTCTCTAGATGCTTCTCCACAGTTTTGGAGGGTTTGAAGTATGCAAATTCACATGAGTGGGTTAAACATGAAGGCTCTGTTGCCACCATTGGCATCACTGCCCATGCTCAGGACCATTTAGGTGAAGTGGTGTTTGTTGAACTGCCAGAGGACAATACTTCAGTGAGCAAAGAGAAAAGCTTTGGAGCAGTGGAGAGTGTGAAGGCAACAAGTGAGATCTTATCACCAATCTCAGGTGAAATCATTGAGGTTAACAAGAAGCTCACAGAATCACCTGGCTTGATCAACTCAAGCCCCTATGAAGATGGTTGGATGATCAAAGTGAAACCAAGTAGCCCCGCGGAGTTGGAATCTTTGATGGGTCCAAAGGAATACACCAAGTTCTGCGAGGAGGAAGATGCTGCTCACTAG

Protein Analysis

165

Amino Acids

17.95

Weight (kDa)

5.24

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 41 - 160 5.8e-51 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32470 AT2G35370
fragaria_vesca FvH4_5g21610 FvH4_5g21610
malus_domestica MD06G1031000.v1.1 MD16G1277700.v1.1
prunus_persica Prupe.5G041300_v2.0.a1
pyrus_communis pycom06g02580 pycom16g24690
rosa_chinensis RchiOBHm_Chr7g0209071
rosa_laevigata RLG00000003170
rosa_multiflora Rmu_co8473347.1_g000001
rosa_roxburghii Rroxscaffold_3G00249780
rosa_rugosa Rorug07G0108700
rosa_samantha Rh7AG243200 Rh7BG238200 Rh7CG258400 Rh7DG250200
rosa_wichuraiana Rw7G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 428
AciI CCGC 2 cut(s) 426, 428
AcsI RAATTY 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 231
AfiI CCNNNNNNNGG 2 cut(s) 196, 320
AgsI TTSAA 2 cut(s) 124, 227
AjnI CCWGG 1 cut(s) 361
AleI CACNNNNGTG 1 cut(s) 142
AluBI AGCT 3 cut(s) 46, 267, 349
AluI AGCT 3 cut(s) 46, 267, 349
Alw26I GTCTC 1 cut(s) 72
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 1 cut(s) 133
AspS9I GGNCC 2 cut(s) 199, 449
AsuHPI GGTGA 4 cut(s) 221, 303, 335, 351
AvaII GGWCC 2 cut(s) 199, 449
BbvI GCAGC 1 cut(s) 475
BccI CCATC 2 cut(s) 386, 439
BciT130I CCWGG 1 cut(s) 363
BclI TGATCA 2 cut(s) 369, 402
BcoDI GTCTC 1 cut(s) 72
BfaI CTAG 2 cut(s) 93, 496
BfmI CTRYAG 1 cut(s) 27
BglII AGATCT 1 cut(s) 303
BisI GCNGC 1 cut(s) 489
BlsI GCNGC 1 cut(s) 490
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 2 cut(s) 199, 449
BmgT120I GGNCC 2 cut(s) 199, 449
BmiI GGNNCC 1 cut(s) 450
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 3 cut(s) 86, 189, 475
Bpu10I CCTNAGC 1 cut(s) 195
BpuEI CTTGAG 2 cut(s) 26, 361
BsaJI CCNNGG 1 cut(s) 426
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 320
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 426
BseGI GGATG 1 cut(s) 405
BseLI CCNNNNNNNGG 2 cut(s) 196, 320
BseMII CTCAG 2 cut(s) 209, 333
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 1 cut(s) 475
Bsh1236I CGCG 1 cut(s) 428
BslI CCNNNNNNNGG 2 cut(s) 196, 320
BsmAI GTCTC 1 cut(s) 72
Bsp143I GATC 3 cut(s) 303, 369, 402
BspACI CCGC 2 cut(s) 426, 428
BspCNI CTCAG 2 cut(s) 208, 332
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 1 cut(s) 450
BssECI CCNNGG 1 cut(s) 426
BssMI GATC 3 cut(s) 303, 369, 402
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 109
BstDEI CTNAG 3 cut(s) 8, 195, 319
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 1 cut(s) 405
BstFNI CGCG 1 cut(s) 428
BstKTI GATC 3 cut(s) 306, 372, 405
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 3 cut(s) 303, 369, 402
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 27
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 1 cut(s) 475
BstX2I RGATCY 1 cut(s) 303
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 1 cut(s) 303
BtgI CCRYGG 1 cut(s) 426
BtsCI GGATG 1 cut(s) 405
BtsI GCAGTG 2 cut(s) 183, 282
BtsIMutI CAGTG 3 cut(s) 183, 255, 282
Cfr13I GGNCC 2 cut(s) 199, 449
Cfr42I CCGCGG 1 cut(s) 429
CviAII CATG 3 cut(s) 140, 155, 191
CviJI RGCY 8 cut(s) 20, 46, 162, 267, 349, 366, 381, 423
CviKI_1 RGCY 8 cut(s) 20, 46, 162, 267, 349, 366, 381, 423
DdeI CTNAG 3 cut(s) 8, 195, 319
DpnI GATC 3 cut(s) 305, 371, 404
DpnII GATC 3 cut(s) 303, 369, 402
Eco47I GGWCC 2 cut(s) 199, 449
Eco57I CTGAAG 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 361
FaeI CATG 3 cut(s) 143, 158, 194
FaiI YATR 5 cut(s) 129, 141, 156, 192, 387
FatI CATG 3 cut(s) 139, 154, 190
FauI CCCGC 1 cut(s) 433
FbaI TGATCA 2 cut(s) 369, 402
Fnu4HI GCNGC 1 cut(s) 489
FokI GGATG 1 cut(s) 412
Fsp4HI GCNGC 1 cut(s) 489
FspBI CTAG 2 cut(s) 93, 496
GluI GCNGC 1 cut(s) 489
Hin1II CATG 3 cut(s) 143, 158, 194
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 2 cut(s) 44, 265
HinfI GANTC 2 cut(s) 356, 437
HpaI GTTAAC 1 cut(s) 340
HphI GGTGA 4 cut(s) 221, 303, 335, 351
Hpy166II GTNNAC 1 cut(s) 340
Hpy188III TCNNGA 2 cut(s) 93, 197
Hpy8I GTNNAC 1 cut(s) 340
HpyAV CCTTC 3 cut(s) 97, 152, 283
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 1 cut(s) 131
HpyF3I CTNAG 3 cut(s) 8, 195, 319
Hsp92II CATG 3 cut(s) 143, 158, 194
Ksp22I TGATCA 2 cut(s) 369, 402
KspAI GTTAAC 1 cut(s) 340
KspI CCGCGG 1 cut(s) 429
Kzo9I GATC 3 cut(s) 303, 369, 402
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 5 cut(s) 182, 246, 306, 348, 375
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 3 cut(s) 86, 189, 475
MaeI CTAG 2 cut(s) 93, 496
MalI GATC 3 cut(s) 305, 371, 404
MboI GATC 3 cut(s) 303, 369, 402
MboII GAAGA 5 cut(s) 15, 42, 45, 401, 494
MflI RGATCY 1 cut(s) 303
MluCI AATT 1 cut(s) 133
MmeI TCCRAC 2 cut(s) 377, 414
MnlI CCTC 5 cut(s) 109, 229, 328, 469, 472
MseI TTAA 2 cut(s) 150, 339
MslI CAYNNNNRTG 2 cut(s) 142, 207
MspA1I CMGCKG 1 cut(s) 428
MspR9I CCNGG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 428
NdeII GATC 3 cut(s) 303, 369, 402
NlaIII CATG 3 cut(s) 143, 158, 194
NlaIV GGNNCC 1 cut(s) 450
OliI CACNNNNGTG 1 cut(s) 142
PfeI GAWTC 2 cut(s) 356, 437
PkrI GCNGC 1 cut(s) 490
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 450
PspPI GGNCC 2 cut(s) 199, 449
PsuI RGATCY 1 cut(s) 303
RseI CAYNNNNRTG 2 cut(s) 142, 207
SacII CCGCGG 1 cut(s) 429
SaqAI TTAA 2 cut(s) 150, 339
SatI GCNGC 1 cut(s) 489
Sau3AI GATC 3 cut(s) 303, 369, 402
Sau96I GGNCC 2 cut(s) 199, 449
ScrFI CCNGG 1 cut(s) 363
SetI ASST 7 cut(s) 48, 211, 269, 325, 339, 351, 364
SfaNI GCATC 3 cut(s) 86, 189, 475
SfcI CTRYAG 1 cut(s) 27
Sfr303I CCGCGG 1 cut(s) 429
SgrBI CCGCGG 1 cut(s) 429
SinI GGWCC 2 cut(s) 199, 449
SmiMI CAYNNNNRTG 2 cut(s) 142, 207
SmlI CTYRAG 2 cut(s) 41, 376
SmoI CTYRAG 2 cut(s) 41, 376
Sse9I AATT 1 cut(s) 133
SsiI CCGC 2 cut(s) 426, 428
SspMI CTAG 2 cut(s) 93, 496
StyD4I CCNGG 1 cut(s) 361
TaaI ACNGT 1 cut(s) 109
TasI AATT 1 cut(s) 133
TfiI GAWTC 2 cut(s) 356, 437
Tru1I TTAA 2 cut(s) 150, 339
Tru9I TTAA 2 cut(s) 150, 339
TscAI CASTG 3 cut(s) 190, 255, 282
TseI GCWGC 1 cut(s) 488
TspDTI ATGAA 2 cut(s) 171, 402
TspRI CASTG 3 cut(s) 190, 255, 282
VpaK11BI GGWCC 2 cut(s) 199, 449
XapI RAATTY 1 cut(s) 133
XbaI TCTAGA 1 cut(s) 92
XspI CTAG 2 cut(s) 93, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.