FvH4_5g21610

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012278
Physical Location & Seq
Reverse (-)
13039240 .. 13041007
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21610.t2

Sequence Viewer

Length: 501 bp
ATGGCACTGAGAATGTGGGCTTCTTCTACAGCCAATGCTCTCAGAATCTCTGGTGGTGCCTCCAAAGCTCAACTCTCCCCTGCATTCTCTCTTTCCAGATGCTTTTCCACTGTTTTGGAAGGGTTGAAGTATGCATCTTCACATGAATGGGTGAAGCATGAAGGTTCAGTTGCTACAATTGGTATCACTGACCATGCTCAGGACCATCTTGGTGAAGTGGTGTTTGTGGAGCTGCCAGAACCGGGTACTGTAGTCTCCCAAAGCAGCAGCTTCGGAAATGTTGAAAGTGTGAAAGCAACCAGTGATGTAAATTCCCCAATCTCTGGTGAGGTTGTTGAGGTTAACCCGAAGCTTAGTGAAACACCTGGTTTGATCAATACAAGCCCATATGAAGATGGATGGATGATCAAGGTGAAACCCAGCAATCCATCAGAACTAGAATCCTTATTGGGTGCTAAAGAATACACGAAATTCTGCGAGGAAGAAGATGCAGCCCACTAG

Protein Analysis

167

Amino Acids

17.74

Weight (kDa)

4.87

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 42 - 166 1.6e-46 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32470 AT2G35370
fragaria_vesca FvH4_5g21610 FvH4_5g21610
malus_domestica MD06G1031000.v1.1 MD16G1277700.v1.1
prunus_persica Prupe.5G041300_v2.0.a1
pyrus_communis pycom06g02580 pycom16g24690
rosa_chinensis RchiOBHm_Chr7g0209071
rosa_laevigata RLG00000003170
rosa_multiflora Rmu_co8473347.1_g000001
rosa_roxburghii Rroxscaffold_3G00249780
rosa_rugosa Rorug07G0108700
rosa_samantha Rh7AG243200 Rh7BG238200 Rh7CG258400 Rh7DG250200
rosa_wichuraiana Rw7G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AccB7I CCANNNNNTGG 1 cut(s) 323
AcsI RAATTY 2 cut(s) 310, 470
AfaI GTAC 1 cut(s) 247
AfiI CCNNNNNNNGG 3 cut(s) 199, 242, 323
AgsI TTSAA 2 cut(s) 127, 284
AjnI CCWGG 1 cut(s) 364
AluBI AGCT 4 cut(s) 68, 232, 270, 352
AluI AGCT 4 cut(s) 68, 232, 270, 352
Alw26I GTCTC 1 cut(s) 259
ApeKI GCWGC 4 cut(s) 232, 264, 267, 491
ApoI RAATTY 2 cut(s) 310, 470
AspS9I GGNCC 1 cut(s) 202
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 4 cut(s) 163, 224, 338, 424
AvaII GGWCC 1 cut(s) 202
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BanI GGYRCC 1 cut(s) 56
BbvI GCAGC 3 cut(s) 219, 276, 279
BccI CCATC 4 cut(s) 213, 389, 393, 436
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 1 cut(s) 366
BclI TGATCA 2 cut(s) 372, 405
BcnI CCSGG 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 2 cut(s) 437, 499
BfmI CTRYAG 2 cut(s) 27, 249
BisI GCNGC 4 cut(s) 233, 265, 268, 492
BlsI GCNGC 4 cut(s) 234, 266, 269, 493
Bme1390I CCNGG 2 cut(s) 243, 366
Bme18I GGWCC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 2 cut(s) 243, 366
BmsI GCATC 3 cut(s) 89, 143, 478
Bpu10I CCTNAGC 1 cut(s) 198
BpuMI CCSGG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 242, 323
Bse1I ACTGG 1 cut(s) 300
BseBI CCWGG 1 cut(s) 366
BseGI GGATG 2 cut(s) 404, 408
BseLI CCNNNNNNNGG 3 cut(s) 199, 242, 323
BseMII CTCAG 2 cut(s) 55, 212
BseNI ACTGG 1 cut(s) 300
BseXI GCAGC 3 cut(s) 219, 276, 279
BseYI CCCAGC 1 cut(s) 419
BshNI GGYRCC 1 cut(s) 56
BsiSI CCGG 1 cut(s) 242
BslI CCNNNNNNNGG 3 cut(s) 199, 242, 323
BsmAI GTCTC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 83
Bsp143I GATC 2 cut(s) 372, 405
BspCNI CTCAG 2 cut(s) 54, 211
BspLI GGNNCC 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 56
BsrI ACTGG 1 cut(s) 300
BssMI GATC 2 cut(s) 372, 405
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 2 cut(s) 112, 250
BstDEI CTNAG 4 cut(s) 8, 41, 198, 353
BstF5I GGATG 2 cut(s) 404, 408
BstKTI GATC 2 cut(s) 375, 408
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 2 cut(s) 372, 405
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 1 cut(s) 366
BstSCI CCNGG 2 cut(s) 241, 364
BstSFI CTRYAG 2 cut(s) 27, 249
BstV1I GCAGC 3 cut(s) 219, 276, 279
BstXI CCANNNNNNTGG 1 cut(s) 115
BtsCI GGATG 2 cut(s) 404, 408
BtsIMutI CAGTG 4 cut(s) 5, 108, 186, 307
Cfr13I GGNCC 1 cut(s) 202
CsiI ACCWGGT 1 cut(s) 364
Csp6I GTAC 1 cut(s) 246
CviAII CATG 3 cut(s) 143, 158, 194
CviJI RGCY 8 cut(s) 20, 32, 68, 232, 270, 352, 384, 494
CviKI_1 RGCY 8 cut(s) 20, 32, 68, 232, 270, 352, 384, 494
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 4 cut(s) 8, 41, 198, 353
DpnI GATC 2 cut(s) 374, 407
DpnII GATC 2 cut(s) 372, 405
Eco47I GGWCC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 364
EcoT22I ATGCAT 1 cut(s) 136
FaeI CATG 3 cut(s) 146, 161, 197
FaiI YATR 6 cut(s) 132, 144, 159, 195, 388, 390
FatI CATG 3 cut(s) 142, 157, 193
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 2 cut(s) 372, 405
Fnu4HI GCNGC 4 cut(s) 233, 265, 268, 492
FokI GGATG 2 cut(s) 411, 415
Fsp4HI GCNGC 4 cut(s) 233, 265, 268, 492
FspBI CTAG 2 cut(s) 437, 499
GluI GCNGC 4 cut(s) 233, 265, 268, 492
GsaI CCCAGC 1 cut(s) 423
HapII CCGG 1 cut(s) 242
Hin1II CATG 3 cut(s) 146, 161, 197
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 2 cut(s) 45, 440
HpaI GTTAAC 1 cut(s) 343
HpaII CCGG 1 cut(s) 242
HphI GGTGA 4 cut(s) 163, 224, 338, 424
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 3 cut(s) 44, 275, 433
Hpy188III TCNNGA 2 cut(s) 96, 200
Hpy8I GTNNAC 1 cut(s) 343
HpyAV CCTTC 2 cut(s) 113, 155
HpyCH4III ACNGT 2 cut(s) 112, 250
HpyCH4V TGCA 3 cut(s) 83, 134, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 4 cut(s) 8, 41, 198, 353
Hsp92II CATG 3 cut(s) 146, 161, 197
Ksp22I TGATCA 2 cut(s) 372, 405
KspAI GTTAAC 1 cut(s) 343
Kzo9I GATC 2 cut(s) 372, 405
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 3 cut(s) 219, 276, 279
LweI GCATC 3 cut(s) 89, 143, 478
MabI ACCWGGT 1 cut(s) 364
MaeI CTAG 2 cut(s) 437, 499
MalI GATC 2 cut(s) 374, 407
MboI GATC 2 cut(s) 372, 405
MboII GAAGA 5 cut(s) 15, 129, 404, 494, 497
MfeI CAATTG 1 cut(s) 177
MluCI AATT 3 cut(s) 177, 310, 470
MnlI CCTC 4 cut(s) 70, 322, 331, 472
Mph1103I ATGCAT 1 cut(s) 136
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 2 cut(s) 145, 210
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 2 cut(s) 243, 366
MunI CAATTG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 65
NciI CCSGG 1 cut(s) 243
NdeI CATATG 1 cut(s) 388
NdeII GATC 2 cut(s) 372, 405
NlaIII CATG 3 cut(s) 146, 161, 197
NlaIV GGNNCC 1 cut(s) 58
NsiI ATGCAT 1 cut(s) 136
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 2 cut(s) 45, 440
PflMI CCANNNNNTGG 1 cut(s) 323
PkrI GCNGC 4 cut(s) 234, 266, 269, 493
Psp6I CCWGG 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 1 cut(s) 202
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
RseI CAYNNNNRTG 2 cut(s) 145, 210
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 4 cut(s) 233, 265, 268, 492
Sau3AI GATC 2 cut(s) 372, 405
Sau96I GGNCC 1 cut(s) 202
ScrFI CCNGG 2 cut(s) 243, 366
SetI ASST 9 cut(s) 70, 166, 234, 272, 333, 342, 354, 367, 414
SexAI ACCWGGT 1 cut(s) 364
SfaNI GCATC 3 cut(s) 89, 143, 478
SfcI CTRYAG 2 cut(s) 27, 249
SinI GGWCC 1 cut(s) 202
SmiMI CAYNNNNRTG 2 cut(s) 145, 210
Sse9I AATT 3 cut(s) 177, 310, 470
SspMI CTAG 2 cut(s) 437, 499
StyD4I CCNGG 2 cut(s) 241, 364
TaaI ACNGT 2 cut(s) 112, 250
TasI AATT 3 cut(s) 177, 310, 470
TfiI GAWTC 2 cut(s) 45, 440
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 4 cut(s) 12, 115, 193, 307
TseI GCWGC 4 cut(s) 232, 264, 267, 491
TspDTI ATGAA 3 cut(s) 159, 174, 405
TspRI CASTG 4 cut(s) 12, 115, 193, 307
Van91I CCANNNNNTGG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 202
XapI RAATTY 2 cut(s) 310, 470
XspI CTAG 2 cut(s) 437, 499
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.