AT1G34150

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003550
Physical Location & Seq
Forward (+)
12435882 .. 12439499
3618 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G34150.1

Sequence Viewer

Length: 1341 bp
ATGACTATCTCCGACGCAAAATCCGATCCCGGCGGCGATGACCGAGACTCTGAAATCGCCAAAGAAACAGAACTAGTGTTTCTCCGTAATCGCGTCAAGGAGTTAGAAGTGGAAAATGCTAAATTGTTGTCTCAGGTTTCAAGCTGCCAATGCCAACAGATGGAAGTGAAGCATGATCGGTCATTGTCAGATTCCAGTAGCTTAGTTAGGAGAAGGAGAGTTAGAAAAGGAGATAAGAATAGTATACCGAGTCATCTTATCTCAAAGAGATATGTTGCACTCAAAATCATGTATTTTGGTAAGAGATTTTATGGATTTTCGGCTGAAGCACAAATGGAACCAAGTATTGAGTCAGAAGTTTTTAAAGCACTTGAAAGGACACGGCTTTTAGTTGGGGACAAGAAAGATTCTTGTTACTCGAGATGTGGGAGAACAGATAAAGGTGTTTCCTCCACAGGGCAGGTGATTGCTTTGTTTTTAAGATCCAGACTGAAGTCACCTCCTGGAGACTCTAAAGCCCAAGTGAATGGGAGAACGGGTGAAAGACCAGAATATGATTATGTAAGAGTTCTAAATCGTGCTCTCCCTGATGACATCCGAGTTATTGGATGGTCTCCTGCTCCTATCGACTTTCATGCAAGGTTTAGTTGCTATGCTAGAGAATACAAATATTTTTTCTGGAGGCAGAACCTGAATCTTTCGGCCATGGACTTTGCCGGGAAGAAGTTCATTGGGGAGCATGATTTCAGAAACTTCTGCAAGATGGATGTGGCAAATGTACATTGCTATACACGGCGTGTTACTTTCTTTGAAGTCTCCCCTTGTCAAAATAGTCATGAGGGTGATCAGCTTTGCACATTTACAATGAGAGGCAGTGCTTTCCTGTGGCACCAGATCCGCTGTATGGTCGCCGTGCTATTCATGATTGGGCAGGGCGTTGAATCAGTTGATGTGATAGATACATTGTTGGACACAAAGAAAACGCCAAGAAAACCTCAATACCTATTGGCCTCAGAGATACCCTTAGTCCTCCGTACATGTGAATTCGAAAATGTTGACTTCATATGTTCTCCAGGTGCTGCAGAGTCACTGCGGTCTCATTTCAAGAATGAATCATTGACATACCAATTAGAGTCTGTAATTTATCAGGAGGCTCTCCGAAACTGTTTACCTCTAAGCAACAATGTTTCAACAGAGGAAAGCTCATGCAATGGCGTAGAAAAAAAGAAGAAAAGGGCAGAACACGTTCCCCTGTTATCTCGACCAACTGAGCCGTCGTATGAAGAACGAACTGCGAAACTGAAACCAAGGAAAGAAGAAACTTTGGCATGTGTAGTGTAA

Protein Analysis

446

Amino Acids

50.98

Weight (kDa)

8.84

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_1 PF01416 241 - 350 1.9e-27 tRNA pseudouridine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 451
Acc36I ACCTGC 1 cut(s) 451
AccB1I GGYRCC 1 cut(s) 888
AccB7I CCANNNNNTGG 1 cut(s) 160
AccI GTMKAC 1 cut(s) 244
AccII CGCG 1 cut(s) 93
AciI CCGC 3 cut(s) 33, 898, 1093
AclWI GGATC 3 cut(s) 20, 477, 889
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 1 cut(s) 1043
AcuI CTGAAG 2 cut(s) 345, 512
AdeI CACNNNGTG 1 cut(s) 797
AfaI GTAC 2 cut(s) 780, 1036
AfiI CCNNNNNNNGG 3 cut(s) 160, 456, 904
AflIII ACRYGT 2 cut(s) 1037, 1243
AgsI TTSAA 6 cut(s) 141, 374, 812, 941, 1105, 1191
AhlI ACTAGT 1 cut(s) 73
AjnI CCWGG 2 cut(s) 502, 1072
AluBI AGCT 4 cut(s) 144, 201, 850, 1203
AluI AGCT 4 cut(s) 144, 201, 850, 1203
Alw21I GWGCWC 1 cut(s) 583
Alw26I GTCTC 6 cut(s) 39, 135, 501, 618, 820, 1101
AlwI GGATC 3 cut(s) 20, 477, 889
AlwNI CAGNNNCTG 2 cut(s) 691, 1079
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 2 cut(s) 702, 1008
ApeKI GCWGC 2 cut(s) 144, 1079
ApoI RAATTY 1 cut(s) 1043
Asp700I GAANNNNTTC 2 cut(s) 725, 1245
AsuC2I CCSGG 2 cut(s) 30, 718
AsuHPI GGTGA 4 cut(s) 475, 489, 551, 854
AsuII TTCGAA 1 cut(s) 1047
AvaI CYCGRG 1 cut(s) 418
BanI GGYRCC 1 cut(s) 888
Bbv12I GWGCWC 1 cut(s) 583
BbvI GCAGC 2 cut(s) 131, 1066
BccI CCATC 3 cut(s) 154, 603, 757
BceAI ACGGC 4 cut(s) 398, 809, 896, 1258
BcgI CGANNNNNNTGC 2 cut(s) 617, 651
BciT130I CCWGG 2 cut(s) 504, 1074
BclI TGATCA 1 cut(s) 844
BcnI CCSGG 2 cut(s) 30, 718
BcoDI GTCTC 6 cut(s) 39, 135, 501, 618, 820, 1101
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 2 cut(s) 74, 657
BfmI CTRYAG 1 cut(s) 1080
BfuAI ACCTGC 1 cut(s) 451
BisI GCNGC 3 cut(s) 34, 145, 1080
BlsI GCNGC 3 cut(s) 35, 146, 1081
Bme1390I CCNGG 4 cut(s) 30, 504, 718, 1074
BmeT110I CYCGRG 1 cut(s) 418
BmiI GGNNCC 2 cut(s) 339, 890
BmrFI CCNGG 4 cut(s) 30, 504, 718, 1074
BoxI GACNNNNGTC 1 cut(s) 493
BplI GAGNNNNNCTC 2 cut(s) 1187, 1219
BpmI CTGGAG 3 cut(s) 525, 700, 1056
Bpu14I TTCGAA 1 cut(s) 1047
BpuMI CCSGG 2 cut(s) 30, 718
BsaI GGTCTC 2 cut(s) 618, 1101
BsaJI CCNNGG 2 cut(s) 705, 1307
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 456, 904
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 2 cut(s) 781, 1216
BseBI CCWGG 2 cut(s) 504, 1074
BseDI CCNNGG 2 cut(s) 705, 1307
BseGI GGATG 3 cut(s) 594, 614, 772
BseLI CCNNNNNNNGG 3 cut(s) 160, 456, 904
BseMI GCAATG 2 cut(s) 781, 1216
BseMII CTCAG 3 cut(s) 146, 1026, 1260
BseNI ACTGG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 131, 1066
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 704, 1010
BshNI GGYRCC 1 cut(s) 888
BsiHKAI GWGCWC 1 cut(s) 583
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 2 cut(s) 30, 717
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 3 cut(s) 160, 456, 904
BsmAI GTCTC 6 cut(s) 39, 135, 501, 618, 820, 1101
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 2 cut(s) 704, 1010
Bso31I GGTCTC 2 cut(s) 618, 1101
BsoBI CYCGRG 1 cut(s) 418
Bsp119I TTCGAA 1 cut(s) 1047
Bsp1286I GDGCHC 1 cut(s) 583
Bsp1407I TGTACA 1 cut(s) 778
Bsp143I GATC 5 cut(s) 25, 175, 482, 844, 894
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 3 cut(s) 33, 898, 1093
BspANI GGCC 2 cut(s) 704, 1010
BspCNI CTCAG 3 cut(s) 145, 1025, 1261
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 2 cut(s) 835, 921
BspLI GGNNCC 2 cut(s) 339, 890
BspMAI CTGCAG 1 cut(s) 1084
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 3 cut(s) 20, 477, 889
BspT104I TTCGAA 1 cut(s) 1047
BspT107I GGYRCC 1 cut(s) 888
BspTNI GGTCTC 2 cut(s) 618, 1101
BsrDI GCAATG 2 cut(s) 781, 1216
BsrGI TGTACA 1 cut(s) 778
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 2 cut(s) 705, 1307
BssMI GATC 5 cut(s) 25, 175, 482, 844, 894
BssNAI GTATAC 1 cut(s) 245
BssT1I CCWWGG 2 cut(s) 705, 1307
Bst1107I GTATAC 1 cut(s) 245
Bst2UI CCWGG 2 cut(s) 504, 1074
Bst4CI ACNGT 1 cut(s) 1166
BstAUI TGTACA 1 cut(s) 778
BstBI TTCGAA 1 cut(s) 1047
BstDEI CTNAG 6 cut(s) 132, 202, 1012, 1024, 1175, 1269
BstDSI CCRYGG 1 cut(s) 705
BstF5I GGATG 3 cut(s) 594, 614, 772
BstFNI CGCG 1 cut(s) 93
BstKTI GATC 5 cut(s) 28, 178, 485, 847, 897
BstMAI GTCTC 6 cut(s) 39, 135, 501, 618, 820, 1101
BstMBI GATC 5 cut(s) 25, 175, 482, 844, 894
BstMWI GCNNNNNNNGC 1 cut(s) 150
BstNI CCWGG 2 cut(s) 504, 1074
BstNSI RCATGY 2 cut(s) 1041, 1332
BstPAI GACNNNNGTC 1 cut(s) 493
BstSCI CCNGG 4 cut(s) 28, 502, 716, 1072
BstSFI CTRYAG 1 cut(s) 1080
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 131, 1066
BstX2I RGATCY 2 cut(s) 482, 894
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 2 cut(s) 482, 894
BstZ17I GTATAC 1 cut(s) 245
BsuRI GGCC 2 cut(s) 704, 1010
BtgI CCRYGG 1 cut(s) 705
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 3 cut(s) 594, 614, 772
BtsI GCAGTG 2 cut(s) 880, 1088
BtsIMutI CAGTG 2 cut(s) 880, 1088
BveI ACCTGC 1 cut(s) 451
CaiI CAGNNNCTG 2 cut(s) 691, 1079
CciI TCATGA 2 cut(s) 835, 921
CseI GACGC 2 cut(s) 23, 82
Csp6I GTAC 2 cut(s) 779, 1035
CviQI GTAC 2 cut(s) 779, 1035
DdeI CTNAG 6 cut(s) 132, 202, 1012, 1024, 1175, 1269
DpnI GATC 5 cut(s) 27, 177, 484, 846, 896
DpnII GATC 5 cut(s) 25, 175, 482, 844, 894
DraI TTTAAA 1 cut(s) 364
DraIII CACNNNGTG 1 cut(s) 797
EaeI YGGCCR 1 cut(s) 702
Eco130I CCWWGG 2 cut(s) 705, 1307
Eco31I GGTCTC 2 cut(s) 618, 1101
Eco57I CTGAAG 2 cut(s) 345, 512
Eco88I CYCGRG 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 1043
EcoRII CCWGG 2 cut(s) 502, 1072
EcoT14I CCWWGG 2 cut(s) 705, 1307
ErhI CCWWGG 2 cut(s) 705, 1307
FaqI GGGAC 1 cut(s) 410
FauNDI CATATG 1 cut(s) 1064
FbaI TGATCA 1 cut(s) 844
FblI GTMKAC 1 cut(s) 244
Fnu4HI GCNGC 3 cut(s) 34, 145, 1080
FokI GGATG 3 cut(s) 581, 621, 779
Fsp4HI GCNGC 3 cut(s) 34, 145, 1080
FspBI CTAG 2 cut(s) 74, 657
GluI GCNGC 3 cut(s) 34, 145, 1080
GsuI CTGGAG 3 cut(s) 525, 700, 1056
HaeIII GGCC 2 cut(s) 704, 1010
HapII CCGG 2 cut(s) 30, 717
HgaI GACGC 2 cut(s) 23, 82
HincII GTYRAC 1 cut(s) 1057
HindII GTYRAC 1 cut(s) 1057
HpaII CCGG 2 cut(s) 30, 717
HphI GGTGA 4 cut(s) 475, 489, 551, 854
Hpy166II GTNNAC 3 cut(s) 245, 1057, 1169
Hpy188I TCNGA 9 cut(s) 13, 25, 52, 190, 355, 599, 749, 1015, 1160
Hpy188III TCNNGA 8 cut(s) 420, 486, 679, 836, 922, 1105, 1148, 1260
Hpy8I GTNNAC 3 cut(s) 245, 1057, 1169
Hpy99I CGWCG 2 cut(s) 17, 1279
HpyAV CCTTC 1 cut(s) 207
HpyCH4III ACNGT 1 cut(s) 1166
HpyCH4IV ACGT 1 cut(s) 1245
HpyCH4V TGCA 6 cut(s) 278, 638, 759, 855, 1082, 1209
HpyF10VI GCNNNNNNNGC 1 cut(s) 150
HpyF3I CTNAG 6 cut(s) 132, 202, 1012, 1024, 1175, 1269
HpySE526I ACGT 1 cut(s) 1245
Ksp22I TGATCA 1 cut(s) 844
Kzo9I GATC 5 cut(s) 25, 175, 482, 844, 894
LmnI GCTCC 2 cut(s) 625, 736
Lsp1109I GCAGC 2 cut(s) 131, 1066
MaeI CTAG 2 cut(s) 74, 657
MaeII ACGT 1 cut(s) 1245
MaeIII GTNAC 4 cut(s) 413, 495, 799, 1086
MalI GATC 5 cut(s) 27, 177, 484, 846, 896
MboI GATC 5 cut(s) 25, 175, 482, 844, 894
MboII GAAGA 4 cut(s) 733, 1240, 1295, 1328
MflI RGATCY 2 cut(s) 482, 894
MhlI GDGCHC 1 cut(s) 583
MluCI AATT 4 cut(s) 122, 1043, 1127, 1140
MlyI GAGTC 6 cut(s) 41, 259, 359, 503, 1094, 1142
MmeI TCCRAC 2 cut(s) 36, 948
MroXI GAANNNNTTC 2 cut(s) 725, 1245
MseI TTAA 2 cut(s) 363, 479
MslI CAYNNNNRTG 1 cut(s) 840
MspA1I CMGCKG 1 cut(s) 900
MspI CCGG 2 cut(s) 30, 717
MspR9I CCNGG 4 cut(s) 30, 504, 718, 1074
MvaI CCWGG 2 cut(s) 504, 1074
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 150
NciI CCSGG 2 cut(s) 30, 718
NcoI CCATGG 1 cut(s) 705
NdeI CATATG 1 cut(s) 1064
NdeII GATC 5 cut(s) 25, 175, 482, 844, 894
NlaIV GGNNCC 2 cut(s) 339, 890
NmuCI GTSAC 2 cut(s) 495, 1086
NspI RCATGY 2 cut(s) 1041, 1332
NspV TTCGAA 1 cut(s) 1047
PaeR7I CTCGAG 1 cut(s) 418
PagI TCATGA 2 cut(s) 835, 921
PaqCI CACCTGC 1 cut(s) 451
PciI ACATGT 1 cut(s) 1037
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PdmI GAANNNNTTC 2 cut(s) 725, 1245
PfeI GAWTC 5 cut(s) 191, 407, 694, 941, 1112
PflMI CCANNNNNTGG 1 cut(s) 160
PfoI TCCNGGA 1 cut(s) 502
PkrI GCNGC 3 cut(s) 35, 146, 1081
PleI GAGTC 6 cut(s) 41, 258, 358, 503, 1093, 1141
PpsI GAGTC 6 cut(s) 41, 258, 358, 503, 1093, 1141
PscI ACATGT 1 cut(s) 1037
PshAI GACNNNNGTC 1 cut(s) 493
Psp6I CCWGG 2 cut(s) 502, 1072
PspGI CCWGG 2 cut(s) 502, 1072
PspN4I GGNNCC 2 cut(s) 339, 890
PstI CTGCAG 1 cut(s) 1084
PstNI CAGNNNCTG 2 cut(s) 691, 1079
PsuI RGATCY 2 cut(s) 482, 894
RsaI GTAC 2 cut(s) 780, 1036
RsaNI GTAC 2 cut(s) 779, 1035
RseI CAYNNNNRTG 1 cut(s) 840
SaqAI TTAA 2 cut(s) 363, 479
SatI GCNGC 3 cut(s) 34, 145, 1080
Sau3AI GATC 5 cut(s) 25, 175, 482, 844, 894
SchI GAGTC 6 cut(s) 41, 259, 359, 503, 1094, 1142
ScrFI CCNGG 4 cut(s) 30, 504, 718, 1074
SduI GDGCHC 1 cut(s) 583
SfcI CTRYAG 1 cut(s) 1080
Sfr274I CTCGAG 1 cut(s) 418
SfuI TTCGAA 1 cut(s) 1047
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 840
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
SpeI ACTAGT 1 cut(s) 73
Sse9I AATT 4 cut(s) 122, 1043, 1127, 1140
SsiI CCGC 3 cut(s) 33, 898, 1093
SspI AATATT 1 cut(s) 671
SspMI CTAG 2 cut(s) 74, 657
StyD4I CCNGG 4 cut(s) 28, 502, 716, 1072
StyI CCWWGG 2 cut(s) 705, 1307
TaaI ACNGT 1 cut(s) 1166
TaiI ACGT 1 cut(s) 1248
TaqI TCGA 4 cut(s) 419, 627, 1047, 1261
TaqII GACCGA 2 cut(s) 57, 168
TasI AATT 4 cut(s) 122, 1043, 1127, 1140
TatI WGTACW 1 cut(s) 778
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 5 cut(s) 191, 407, 694, 941, 1112
Tru1I TTAA 2 cut(s) 363, 479
Tru9I TTAA 2 cut(s) 363, 479
TscAI CASTG 2 cut(s) 880, 1095
TseFI GTSAC 2 cut(s) 495, 1086
TseI GCWGC 2 cut(s) 144, 1079
Tsp45I GTSAC 2 cut(s) 495, 1086
TspDTI ATGAA 6 cut(s) 623, 718, 910, 1051, 1125, 1296
TspGWI ACGGA 2 cut(s) 74, 1022
TspRI CASTG 2 cut(s) 880, 1095
Van91I CCANNNNNTGG 1 cut(s) 160
XapI RAATTY 1 cut(s) 1043
XceI RCATGY 2 cut(s) 1041, 1332
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 244
XmnI GAANNNNTTC 2 cut(s) 725, 1245
XspI CTAG 2 cut(s) 74, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.