Rorug06G0076200

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003550
Physical Location & Seq
Reverse (-)
10126695 .. 10128146
1452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0076200.1

Sequence Viewer

Length: 1452 bp
ATGGAAACCAAACCTCATCCGCAGCTTCATATTTTCTTCCTTCCATTTATGGCTCAAGGCCACACTTTACCCATGATAGACATAGCCAAACTATTTGCTTCACGCAGTGTAAAATGCACCATAGTAACCACCCCTGCCAATGCACCAATCATCTCCAAAGCAATCCAAACTAGCAAAGGTTTCGGTTTTGAAATTGAAGTTGTTGTCATCAAATTCCCATCTACTGAAGTTGGGTTGCCTGAAGGAATTGAAAGTACTAAATTGGAGAAGACAGAAGAGATGAGGGAAAAGTTCATAAAAGCAATCACTCTGTTTGAACAACAGGTAGAGAAAATTCTAGACCAACATCATCCTCATTGCCTTGTTGCAGACTTTTTATTTCATTGGGCTACAGATGTTGCTGCAAAGTTTGGGATCCCAAGGTTCGTCTTTCATGGAGCTGGTTTTTTCGCCTTGTGTGGTCTAAAGAGTGTAATGCTGTACCAACCTCACATGAAGGTTTCATCAGATTCAGAATCTTTTGTTATTCCTAGTCTCCCAGATGAGATAATTATGACAAGAAACCAGCTAGCACCTTTTCTTACTCGAAATGGTAAGACAGAACTCATCAAGTTAATCAAAGCATCCAGAGAAGCTGAGGAAAGGAGCTACGGGATTATTGTAAACAGCTACTATGAACTTGAACCTGATTATGCAGATCACTATAGGAAGGTGTTGGGGAAGAAGTTCTGGCATATCGGCCCAGTCTCTTTATGCAACAAGGTAGAAAAAGATAAATCAGAGAGGGGAAGGGAAGGTTCGGTTGATGAAGTACATGAGTGCTTGAACTGGCTTAATTCCAAGAAACCCAATTCGGTTGTTTATATATGTTTTGGAAGTATGACCAAATTCAGTGATTGTCAGCTCATAGAAATTGCCTTCGGTCTTGAGGCTTCTCAGCAGCAATTCATTTGGGTTGTGAATAAAGAAAATAATGATAAAGAAGAGTGGTTACCTGAAGGATTTAAGCAGAGAAATGAGGGTAAGGGACTGGTTATAAGAGGTTGGGCTCCCCAACTACTGATTCTTCAAAATGAAGCAGTTGGGGCCTTTGTCACTCATTGTGGGTGGAACTCAATCCTTGAAGGGGTGTCTTCTGGGGTTCCAATGATCACCTGGCCAGTGTCTGCAGACCAGTTTTTCAATGAGAAGTTGGTGACTCAGATACTTGGCATTGGGATTGCCATTGGTGCTCAAAAGTGGGAGGATGATAGTGTGCAGAGCGAAGCCAATGTGAAGAGGGAGGCTATAGAGAAGGCTGTGACTGAATTCATGGTGGGTAATGAACAAGAGGAAATGAGAAGCAAAGTGCTTGCGCTTGGACAGATGGCAAGGAGGGCAGTTGCAGAAGGTGGTTCATCGTACTCTGATTTAACTGCTCTAATTGAAGAGTTGTGGTTCCTCGTGTCTTGA

Protein Analysis

483

Amino Acids

54.38

Weight (kDa)

5.72

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 274 - 401 6.9e-20 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1037
AciI CCGC 1 cut(s) 20
AclWI GGATC 2 cut(s) 409, 422
AcoI YGGCCR 1 cut(s) 1157
AcsI RAATTY 4 cut(s) 212, 333, 887, 1307
AcuI CTGAAG 3 cut(s) 246, 261, 1017
AdeI CACNNNGTG 1 cut(s) 107
AfaI GTAC 4 cut(s) 256, 482, 813, 1403
AfiI CCNNNNNNNGG 1 cut(s) 1126
AjnI CCWGG 1 cut(s) 1154
AjuI GAANNNNNNNTTGG 2 cut(s) 1047, 1079
AluBI AGCT 7 cut(s) 25, 440, 568, 635, 648, 669, 904
AluI AGCT 7 cut(s) 25, 440, 568, 635, 648, 669, 904
Alw21I GWGCWC 1 cut(s) 1234
Alw26I GTCTC 2 cut(s) 539, 751
AlwI GGATC 2 cut(s) 409, 422
AlwNI CAGNNNCTG 1 cut(s) 1166
AoxI GGCC 4 cut(s) 58, 739, 1086, 1157
ApeKI GCWGC 3 cut(s) 22, 401, 940
ApoI RAATTY 4 cut(s) 212, 333, 887, 1307
Asp700I GAANNNNTTC 1 cut(s) 725
AspLEI GCGC 1 cut(s) 1357
AspS9I GGNCC 2 cut(s) 740, 1086
AsuHPI GGTGA 2 cut(s) 1144, 1207
AsuNHI GCTAGC 1 cut(s) 568
BalI TGGCCA 1 cut(s) 1159
BamHI GGATCC 1 cut(s) 414
BanII GRGCYC 1 cut(s) 1051
BarI GAAGNNNNNNTAC 2 cut(s) 975, 1007
BauI CACGAG 1 cut(s) 1442
BbsI GAAGAC 2 cut(s) 275, 1125
Bbv12I GWGCWC 1 cut(s) 1234
BbvCI CCTCAGC 1 cut(s) 636
BbvI GCAGC 3 cut(s) 34, 388, 952
BccI CCATC 2 cut(s) 226, 1360
BcgI CGANNNNNNTGC 2 cut(s) 163, 197
BciT130I CCWGG 1 cut(s) 1156
BclI TGATCA 1 cut(s) 1149
BcoDI GTCTC 2 cut(s) 539, 751
BfaI CTAG 4 cut(s) 171, 338, 531, 569
BfmI CTRYAG 4 cut(s) 390, 703, 1167, 1287
BisI GCNGC 3 cut(s) 23, 402, 941
BlsI GCNGC 3 cut(s) 24, 403, 942
BmcAI AGTACT 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 1156
BmgT120I GGNCC 2 cut(s) 740, 1086
BmiI GGNNCC 5 cut(s) 416, 1050, 1087, 1143, 1439
BmrFI CCNGG 1 cut(s) 1156
BmrI ACTGGG 1 cut(s) 737
BmsI GCATC 1 cut(s) 632
BmtI GCTAGC 1 cut(s) 572
BmuI ACTGGG 1 cut(s) 737
BpiI GAAGAC 2 cut(s) 275, 1125
Bpu10I CCTNAGC 1 cut(s) 636
BpuEI CTTGAG 2 cut(s) 39, 947
BsaJI CCNNGG 1 cut(s) 419
Bsc4I CCNNNNNNNGG 1 cut(s) 1126
Bse1I ACTGG 5 cut(s) 743, 833, 1035, 1160, 1174
Bse3DI GCAATG 1 cut(s) 355
BseBI CCWGG 1 cut(s) 1156
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 4 cut(s) 16, 349, 623, 1252
BseLI CCNNNNNNNGG 1 cut(s) 1126
BseMI GCAATG 1 cut(s) 355
BseMII CTCAG 3 cut(s) 627, 950, 1214
BseNI ACTGG 5 cut(s) 743, 833, 1035, 1160, 1174
BseXI GCAGC 3 cut(s) 34, 388, 952
BsgI GTGCAG 1 cut(s) 1277
BshFI GGCC 4 cut(s) 60, 741, 1088, 1159
BsiHKAI GWGCWC 1 cut(s) 1234
BslFI GGGAC 1 cut(s) 1041
BslI CCNNNNNNNGG 1 cut(s) 1126
BsmAI GTCTC 2 cut(s) 539, 751
BsmFI GGGAC 1 cut(s) 1041
BsnI GGCC 4 cut(s) 60, 741, 1088, 1159
Bsp1286I GDGCHC 2 cut(s) 1051, 1234
Bsp143I GATC 3 cut(s) 414, 697, 1149
BspACI CCGC 1 cut(s) 20
BspANI GGCC 4 cut(s) 60, 741, 1088, 1159
BspCNI CTCAG 3 cut(s) 628, 949, 1213
BspLI GGNNCC 5 cut(s) 416, 1050, 1087, 1143, 1439
BspMAI CTGCAG 1 cut(s) 1171
BspOI GCTAGC 1 cut(s) 572
BspPI GGATC 2 cut(s) 409, 422
BsrDI GCAATG 1 cut(s) 355
BsrI ACTGG 5 cut(s) 743, 833, 1035, 1160, 1174
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 3 cut(s) 414, 697, 1149
BssSI CACGAG 1 cut(s) 1442
BssT1I CCWWGG 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 1442
Bst2UI CCWGG 1 cut(s) 1156
Bst6I CTCTTC 4 cut(s) 270, 978, 1271, 1422
BstC8I GCNNGC 2 cut(s) 570, 1353
BstDEI CTNAG 3 cut(s) 636, 936, 1200
BstEII GGTNACC 1 cut(s) 990
BstF5I GGATG 4 cut(s) 16, 349, 623, 1252
BstHHI GCGC 1 cut(s) 1357
BstKTI GATC 3 cut(s) 417, 700, 1152
BstMAI GTCTC 2 cut(s) 539, 751
BstMBI GATC 3 cut(s) 414, 697, 1149
BstMWI GCNNNNNNNGC 3 cut(s) 1085, 1229, 1376
BstNI CCWGG 1 cut(s) 1156
BstPI GGTNACC 1 cut(s) 990
BstSCI CCNGG 1 cut(s) 1154
BstSFI CTRYAG 4 cut(s) 390, 703, 1167, 1287
BstV1I GCAGC 3 cut(s) 34, 388, 952
BstV2I GAAGAC 2 cut(s) 275, 1125
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuRI GGCC 4 cut(s) 60, 741, 1088, 1159
BtsCI GGATG 4 cut(s) 16, 349, 623, 1252
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 3 cut(s) 112, 898, 1167
Cac8I GCNNGC 2 cut(s) 570, 1353
CaiI CAGNNNCTG 1 cut(s) 1166
CfoI GCGC 1 cut(s) 1357
Cfr13I GGNCC 2 cut(s) 740, 1086
Csp6I GTAC 4 cut(s) 255, 481, 812, 1402
CviAII CATG 5 cut(s) 73, 434, 493, 815, 1312
CviQI GTAC 4 cut(s) 255, 481, 812, 1402
DdeI CTNAG 3 cut(s) 636, 936, 1200
DpnI GATC 3 cut(s) 416, 699, 1151
DpnII GATC 3 cut(s) 414, 697, 1149
DraIII CACNNNGTG 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 1157
Eam1104I CTCTTC 4 cut(s) 270, 978, 1271, 1422
EarI CTCTTC 4 cut(s) 270, 978, 1271, 1422
Eco130I CCWWGG 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 1051
Eco57I CTGAAG 3 cut(s) 246, 261, 1017
Eco91I GGTNACC 1 cut(s) 990
EcoO109I RGGNCCY 1 cut(s) 1086
EcoO65I GGTNACC 1 cut(s) 990
EcoRI GAATTC 1 cut(s) 1307
EcoRII CCWGG 1 cut(s) 1154
EcoT14I CCWWGG 1 cut(s) 419
EcoT38I GRGCYC 1 cut(s) 1051
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 5 cut(s) 76, 437, 496, 818, 1315
FaqI GGGAC 1 cut(s) 1041
FatI CATG 5 cut(s) 72, 433, 492, 814, 1311
FbaI TGATCA 1 cut(s) 1149
Fnu4HI GCNGC 3 cut(s) 23, 402, 941
FokI GGATG 4 cut(s) 3, 336, 610, 1259
FriOI GRGCYC 1 cut(s) 1051
Fsp4HI GCNGC 3 cut(s) 23, 402, 941
FspBI CTAG 4 cut(s) 171, 338, 531, 569
GlaI GCGC 1 cut(s) 1356
GluI GCNGC 3 cut(s) 23, 402, 941
HaeIII GGCC 4 cut(s) 60, 741, 1088, 1159
HhaI GCGC 1 cut(s) 1357
Hin1II CATG 5 cut(s) 76, 437, 496, 818, 1315
Hin6I GCGC 1 cut(s) 1355
HinP1I GCGC 1 cut(s) 1355
HinfI GANTC 4 cut(s) 509, 515, 1063, 1198
HphI GGTGA 2 cut(s) 1144, 1207
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 5 cut(s) 508, 514, 781, 1203, 1408
Hpy188III TCNNGA 4 cut(s) 338, 627, 926, 1449
Hpy8I GTNNAC 1 cut(s) 664
HpyCH4V TGCA 9 cut(s) 117, 143, 368, 404, 695, 756, 1169, 1258, 1385
HpyF10VI GCNNNNNNNGC 3 cut(s) 1085, 1229, 1376
HpyF3I CTNAG 3 cut(s) 636, 936, 1200
Hsp92II CATG 5 cut(s) 76, 437, 496, 818, 1315
HspAI GCGC 1 cut(s) 1355
Ksp22I TGATCA 1 cut(s) 1149
Kzo9I GATC 3 cut(s) 414, 697, 1149
LmnI GCTCC 3 cut(s) 437, 645, 1054
Lsp1109I GCAGC 3 cut(s) 34, 388, 952
LweI GCATC 1 cut(s) 632
MaeI CTAG 4 cut(s) 171, 338, 531, 569
MaeIII GTNAC 5 cut(s) 124, 990, 1093, 1195, 1300
MalI GATC 3 cut(s) 416, 699, 1151
MboI GATC 3 cut(s) 414, 697, 1149
MboII GAAGA 9 cut(s) 28, 280, 287, 733, 995, 1058, 1125, 1288, 1439
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 2 cut(s) 1051, 1234
MlsI TGGCCA 1 cut(s) 1159
MluNI TGGCCA 1 cut(s) 1159
MlyI GAGTC 1 cut(s) 1192
Mox20I TGGCCA 1 cut(s) 1159
MroXI GAANNNNTTC 1 cut(s) 725
MscI TGGCCA 1 cut(s) 1159
MseI TTAA 4 cut(s) 614, 834, 1005, 1412
Msp20I TGGCCA 1 cut(s) 1159
MspR9I CCNGG 1 cut(s) 1156
MvaI CCWGG 1 cut(s) 1156
MwoI GCNNNNNNNGC 3 cut(s) 1085, 1229, 1376
NdeII GATC 3 cut(s) 414, 697, 1149
NheI GCTAGC 1 cut(s) 568
NlaIII CATG 5 cut(s) 76, 437, 496, 818, 1315
NlaIV GGNNCC 5 cut(s) 416, 1050, 1087, 1143, 1439
NmuCI GTSAC 3 cut(s) 1093, 1195, 1300
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 3 cut(s) 509, 515, 1063
PkrI GCNGC 3 cut(s) 24, 403, 942
PleI GAGTC 1 cut(s) 1192
PpsI GAGTC 1 cut(s) 1192
PsiI TTATAA 1 cut(s) 1037
Psp6I CCWGG 1 cut(s) 1154
PspEI GGTNACC 1 cut(s) 990
PspGI CCWGG 1 cut(s) 1154
PspN4I GGNNCC 5 cut(s) 416, 1050, 1087, 1143, 1439
PspPI GGNCC 2 cut(s) 740, 1086
PstI CTGCAG 1 cut(s) 1171
PstNI CAGNNNCTG 1 cut(s) 1166
PsuI RGATCY 1 cut(s) 414
RsaI GTAC 4 cut(s) 256, 482, 813, 1403
RsaNI GTAC 4 cut(s) 255, 481, 812, 1402
SaqAI TTAA 4 cut(s) 614, 834, 1005, 1412
SatI GCNGC 3 cut(s) 23, 402, 941
Sau3AI GATC 3 cut(s) 414, 697, 1149
Sau96I GGNCC 2 cut(s) 740, 1086
ScaI AGTACT 1 cut(s) 256
SchI GAGTC 1 cut(s) 1192
ScrFI CCNGG 1 cut(s) 1156
SduI GDGCHC 2 cut(s) 1051, 1234
SfaNI GCATC 1 cut(s) 632
SfcI CTRYAG 4 cut(s) 390, 703, 1167, 1287
SmlI CTYRAG 2 cut(s) 54, 926
SmoI CTYRAG 2 cut(s) 54, 926
SsiI CCGC 1 cut(s) 20
SspMI CTAG 4 cut(s) 171, 338, 531, 569
StyD4I CCNGG 1 cut(s) 1154
StyI CCWWGG 1 cut(s) 419
TaqI TCGA 1 cut(s) 586
TaqII GACCGA 1 cut(s) 911
TatI WGTACW 2 cut(s) 254, 811
TfiI GAWTC 3 cut(s) 509, 515, 1063
Tru1I TTAA 4 cut(s) 614, 834, 1005, 1412
Tru9I TTAA 4 cut(s) 614, 834, 1005, 1412
TscAI CASTG 3 cut(s) 112, 898, 1167
TseFI GTSAC 3 cut(s) 1093, 1195, 1300
TseI GCWGC 3 cut(s) 22, 401, 940
Tsp45I GTSAC 3 cut(s) 1093, 1195, 1300
TspRI CASTG 3 cut(s) 112, 898, 1167
XapI RAATTY 4 cut(s) 212, 333, 887, 1307
XbaI TCTAGA 1 cut(s) 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 1152
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 4 cut(s) 171, 338, 531, 569
ZrmI AGTACT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.