AT1G34430

of pyruvate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010794
Physical Location & Seq
Reverse (-)
12587726 .. 12590223
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G34430.1

Sequence Viewer

Length: 1398 bp
ATGTCTCGTCTTCTCCAAACACCCTTCTTACCTTCAGTTTCCCTCCCCACAAAAACACGATCTTCCGTTACGGGTTTCCGGGTAAAGCCCAGAATCATCCCAATTCAAGCAAAGATCCGAGAAATCTTCATGCCTGCACTAAGTTCCACGATGACCGAAGGCAAAATCGTCTCTTGGGTTAAATCAGAAGGCGATAAACTCAACAAAGGAGAGAGTGTTGTTGTTGTTGAATCTGATAAAGCTGATATGGATGTTGAGACTTTCTATGATGGTTATCTAGCTGCAATCATGGTTGAAGAAGGTGGTGTTGCTCCTGTTGGTTCTGCTATTGCTTTGTTAGCTGAAACTGAAGATGAGATTGCTGATGCTAAAGCTAAAGCTTCTGGTGGTGGTGGTGGTGGTGATTCTAAGGCTCCTCCGGCTTCTCCTCCCACGGCTGCAGTTGAAGCACCTGTGAGTGTTGAGAAGAAGGTTGCAGCTGCTCCGGTGTCCATTAAGGCGGTGGCGGCGTCTGCGGTGCATCCGGCGTCTGAAGGAGGGAAGAGGATTGTGGCGTCTCCTTATGCTAAGAAGTTAGCTAAGGAGTTGAAGGTTGAATTGGCTGGTTTAGTTGGAAGTGGACCGATGGGAAGGATTGTGGCTAAAGATGTTGAGGCGGTTGCTGCTGGAGGAGGAGTTCAAGCTGCTGTTGCTGTTAAGGAGGTAGTGGCAGCTCCTGGTGTGGAGTTGGGATCGGTGGTTCCTTTTACGACAATGCAGGGGGCTGTGAGTAGGAATATGGTGGAGAGTCTTGGTGTTCCTACTTTTAGAGTCGGTTACACGATTAGTACGGATGCTCTTGATGCTCTCTACAAGAAGATTAAGTCGAAAGGTGTGACAATGACGGCACTTTTAGCAAAGGCAACTGCACTTGCACTGGCGAAACATCCAGTTGTAAACTCGTCTTGTAGAGATGGTAACAGTTTTGTGTATAATAGTAGCATCAACGTTGCTGTTGCTGTTGCTATTGATGGTGGTTTGATCACTCCGGTGCTTCAGAACGCTGATAAGGTTGACATTTATTCGTTATCTAGAAAATGGAAAGAATTGGTTGATAAGGCTCGAGCCAAGCAGCTGCAGCCTCAGGAGTACAACACTGGTACCTTCACTCTCTCTAACCTAGGAATGTTTGGCGTTGATCGGTTTGATGCTATTCTACCCCCTGGAACAGGAGCGATTATGGCTGTCGGAGCATCTCAACCTTCAGTGGTTGCAACTAAAGACGGCCGCATTGGCATGAAAAACCAGATGCAGGTGAATGTGACGGCAGATCATCGTGTCATCTATGGTGCTGATCTTGCACAGTTCTTGCAAACATTGGCAAGCATTATTGAGGACCCAAAGGACCTTACATTCTAG

Protein Analysis

465

Amino Acids

48.31

Weight (kDa)

8.8

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 41 - 112 3.9e-15 Biotin-requiring enzyme
E3_binding PF02817 183 - 218 6.2e-13 e3 binding domain
2-oxoacid_dh PF00198 246 - 463 2.2e-64 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34430
fragaria_vesca FvH4_2g12130
malus_domestica MD05G1040900.v1.1 MD10G1046900.v1.1
prunus_persica Prupe.8G056000_v2.0.a1
pyrus_communis pycom05g03360 pycom10g03320
rosa_chinensis RchiOBHm_Chr6g0272101 RchiOBHm_Chr6g0272111
rosa_laevigata RLG00000013702
rosa_multiflora Rmu_sc0013301.1_g000004 Rmu_sc0032599.1_g000001
rosa_roxburghii Rroxscaffold_7G00196600
rosa_rugosa Rorug06G0065600
rosa_samantha Rh6AG182900 Rh6BG184200 Rh6CG184100 Rh6DG175700
rosa_wichuraiana Rw0G009140 Rw6G015740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1282
Acc36I ACCTGC 1 cut(s) 1282
Acc65I GGTACC 1 cut(s) 1139
AccB1I GGYRCC 1 cut(s) 1139
AciI CCGC 5 cut(s) 500, 506, 515, 656, 1267
AclI AACGTT 1 cut(s) 987
AclWI GGATC 2 cut(s) 109, 739
AcoI YGGCCR 1 cut(s) 1264
AcuI CTGAAG 5 cut(s) 18, 369, 552, 1019, 1227
AcyI GRCGYC 3 cut(s) 509, 527, 554
AfaI GTAC 3 cut(s) 829, 1130, 1141
AfiI CCNNNNNNNGG 2 cut(s) 1208, 1291
AgsI TTSAA 7 cut(s) 107, 230, 296, 446, 589, 596, 680
AjnI CCWGG 2 cut(s) 715, 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 581, 613
AleI CACNNNNGTG 1 cut(s) 1028
AloI GAACNNNNNNTCC 4 cut(s) 660, 692, 723, 755
Alw26I GTCTC 4 cut(s) 9, 175, 251, 561
AlwI GGATC 2 cut(s) 109, 739
AlwNI CAGNNNCTG 1 cut(s) 716
Ama87I CYCGRG 1 cut(s) 1101
AoxI GGCC 1 cut(s) 1264
Asp718I GGTACC 1 cut(s) 1139
AspA2I CCTAGG 1 cut(s) 1159
AspS9I GGNCC 3 cut(s) 620, 1375, 1384
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 413, 1306
AvaI CYCGRG 1 cut(s) 1101
AvaII GGWCC 3 cut(s) 620, 1375, 1384
AvrII CCTAGG 1 cut(s) 1159
AxyI CCTNAGG 1 cut(s) 1122
BaeI ACNNNNGTAYC 2 cut(s) 1123, 1156
BanI GGYRCC 1 cut(s) 1139
BbsI GAAGAC 1 cut(s) 2
BccI CCATC 4 cut(s) 263, 619, 947, 1004
BceAI ACGGC 4 cut(s) 450, 900, 1279, 1320
BciT130I CCWGG 2 cut(s) 717, 1203
BclI TGATCA 1 cut(s) 1020
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 4 cut(s) 9, 175, 251, 561
BfaI CTAG 4 cut(s) 278, 1071, 1160, 1396
BfmI CTRYAG 2 cut(s) 438, 1115
BfuAI ACCTGC 1 cut(s) 1282
BglI GCCNNNNNGGC 1 cut(s) 1272
BlnI CCTAGG 1 cut(s) 1159
Bme1390I CCNGG 3 cut(s) 80, 717, 1203
Bme18I GGWCC 3 cut(s) 620, 1375, 1384
BmeT110I CYCGRG 1 cut(s) 1101
BmgT120I GGNCC 3 cut(s) 620, 1375, 1384
BmiI GGNNCC 4 cut(s) 414, 741, 1141, 1377
BmrFI CCNGG 3 cut(s) 80, 717, 1203
BmsI GCATC 8 cut(s) 355, 529, 823, 832, 990, 1177, 1241, 1278
BpiI GAAGAC 1 cut(s) 2
BpmI CTGGAG 1 cut(s) 687
Bpu10I CCTNAGC 1 cut(s) 579
BpuMI CCSGG 1 cut(s) 80
BsaBI GATNNNNATC 1 cut(s) 273
BsaHI GRCGYC 3 cut(s) 509, 527, 554
BsaJI CCNNGG 3 cut(s) 432, 1159, 1201
BsaWI WCCGGW 2 cut(s) 484, 1027
BsaXI ACNNNNNCTCC 4 cut(s) 201, 231, 660, 690
Bsc4I CCNNNNNNNGG 2 cut(s) 1208, 1291
Bse1I ACTGG 3 cut(s) 921, 929, 1141
Bse21I CCTNAGG 1 cut(s) 1122
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 2 cut(s) 717, 1203
BseDI CCNNGG 3 cut(s) 432, 1159, 1201
BseGI GGATG 5 cut(s) 96, 256, 520, 838, 925
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 1208, 1291
BseMII CTCAG 1 cut(s) 1136
BseNI ACTGG 3 cut(s) 921, 929, 1141
BseRI GAGGAG 4 cut(s) 405, 417, 684, 687
BseX3I CGGCCG 1 cut(s) 1264
BsgI GTGCAG 2 cut(s) 120, 891
Bsh1285I CGRYCG 1 cut(s) 1267
BshFI GGCC 1 cut(s) 1266
BshNI GGYRCC 1 cut(s) 1139
BsiEI CGRYCG 1 cut(s) 1267
BsiHKCI CYCGRG 1 cut(s) 1101
BsiSI CCGG 5 cut(s) 79, 419, 485, 524, 1028
BslI CCNNNNNNNGG 2 cut(s) 1208, 1291
BsmAI GTCTC 4 cut(s) 9, 175, 251, 561
BsmBI CGTCTC 2 cut(s) 175, 561
BsnI GGCC 1 cut(s) 1266
BsoBI CYCGRG 1 cut(s) 1101
Bsp143I GATC 7 cut(s) 59, 114, 731, 1020, 1177, 1309, 1333
BspACI CCGC 5 cut(s) 500, 506, 515, 656, 1267
BspANI GGCC 1 cut(s) 1266
BspCNI CTCAG 1 cut(s) 1135
BspLI GGNNCC 4 cut(s) 414, 741, 1141, 1377
BspMAI CTGCAG 2 cut(s) 442, 1119
BspMI ACCTGC 1 cut(s) 1282
BspPI GGATC 2 cut(s) 109, 739
BspT107I GGYRCC 1 cut(s) 1139
BsrI ACTGG 3 cut(s) 921, 929, 1141
BssECI CCNNGG 3 cut(s) 432, 1159, 1201
BssMI GATC 7 cut(s) 59, 114, 731, 1020, 1177, 1309, 1333
BssNI GRCGYC 3 cut(s) 509, 527, 554
BssT1I CCWWGG 1 cut(s) 1159
Bst2UI CCWGG 2 cut(s) 717, 1203
Bst4CI ACNGT 2 cut(s) 962, 1344
Bst6I CTCTTC 1 cut(s) 536
BstACI GRCGYC 3 cut(s) 509, 527, 554
BstC8I GCNNGC 2 cut(s) 135, 1363
BstDEI CTNAG 5 cut(s) 140, 408, 567, 579, 1122
BstDSI CCRYGG 1 cut(s) 432
BstENI CCTNNNNNAGG 1 cut(s) 1206
BstF5I GGATG 5 cut(s) 96, 256, 520, 838, 925
BstKTI GATC 7 cut(s) 62, 117, 734, 1023, 1180, 1312, 1336
BstMAI GTCTC 4 cut(s) 9, 175, 251, 561
BstMBI GATC 7 cut(s) 59, 114, 731, 1020, 1177, 1309, 1333
BstMCI CGRYCG 1 cut(s) 1267
BstNI CCWGG 2 cut(s) 717, 1203
BstSCI CCNGG 3 cut(s) 78, 715, 1201
BstSFI CTRYAG 2 cut(s) 438, 1115
BstV2I GAAGAC 1 cut(s) 2
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
BstZI CGGCCG 1 cut(s) 1264
Bsu36I CCTNAGG 1 cut(s) 1122
BsuRI GGCC 1 cut(s) 1266
BtgI CCRYGG 1 cut(s) 432
BtsCI GGATG 5 cut(s) 96, 256, 520, 838, 925
BtsIMutI CAGTG 3 cut(s) 914, 1134, 1251
BveI ACCTGC 1 cut(s) 1282
Cac8I GCNNGC 2 cut(s) 135, 1363
CaiI CAGNNNCTG 1 cut(s) 716
Cfr13I GGNCC 3 cut(s) 620, 1375, 1384
CseI GACGC 3 cut(s) 498, 516, 543
Csp6I GTAC 3 cut(s) 828, 1129, 1140
CviAII CATG 3 cut(s) 130, 289, 1276
CviQI GTAC 3 cut(s) 828, 1129, 1140
DdeI CTNAG 5 cut(s) 140, 408, 567, 579, 1122
DpnI GATC 7 cut(s) 61, 116, 733, 1022, 1179, 1311, 1335
DpnII GATC 7 cut(s) 59, 114, 731, 1020, 1177, 1309, 1333
EaeI YGGCCR 1 cut(s) 1264
EagI CGGCCG 1 cut(s) 1264
Eam1104I CTCTTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 536
EclXI CGGCCG 1 cut(s) 1264
Eco130I CCWWGG 1 cut(s) 1159
Eco47I GGWCC 3 cut(s) 620, 1375, 1384
Eco52I CGGCCG 1 cut(s) 1264
Eco57I CTGAAG 5 cut(s) 18, 369, 552, 1019, 1227
Eco81I CCTNAGG 1 cut(s) 1122
Eco88I CYCGRG 1 cut(s) 1101
EcoNI CCTNNNNNAGG 1 cut(s) 1206
EcoO109I RGGNCCY 2 cut(s) 1375, 1384
EcoRII CCWGG 2 cut(s) 715, 1201
EcoT14I CCWWGG 1 cut(s) 1159
ErhI CCWWGG 1 cut(s) 1159
Esp3I CGTCTC 2 cut(s) 175, 561
FaeI CATG 3 cut(s) 133, 292, 1279
FatI CATG 3 cut(s) 129, 288, 1275
FbaI TGATCA 1 cut(s) 1020
FokI GGATG 5 cut(s) 83, 263, 507, 845, 912
FspBI CTAG 4 cut(s) 278, 1071, 1160, 1396
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 1 cut(s) 1266
HapII CCGG 5 cut(s) 79, 419, 485, 524, 1028
HgaI GACGC 3 cut(s) 498, 516, 543
Hin1I GRCGYC 3 cut(s) 509, 527, 554
Hin1II CATG 3 cut(s) 133, 292, 1279
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 5 cut(s) 93, 230, 404, 787, 810
HpaII CCGG 5 cut(s) 79, 419, 485, 524, 1028
HphI GGTGA 2 cut(s) 413, 1306
Hpy166II GTNNAC 3 cut(s) 620, 937, 1054
Hpy188I TCNGA 6 cut(s) 119, 187, 235, 532, 1038, 1229
Hpy188III TCNNGA 3 cut(s) 839, 1071, 1124
Hpy8I GTNNAC 3 cut(s) 620, 937, 1054
HpyCH4III ACNGT 2 cut(s) 962, 1344
HpyCH4IV ACGT 1 cut(s) 987
HpyF3I CTNAG 5 cut(s) 140, 408, 567, 579, 1122
HpySE526I ACGT 1 cut(s) 987
Hsp92I GRCGYC 3 cut(s) 509, 527, 554
Hsp92II CATG 3 cut(s) 133, 292, 1279
KpnI GGTACC 1 cut(s) 1143
Ksp22I TGATCA 1 cut(s) 1020
Kzo9I GATC 7 cut(s) 59, 114, 731, 1020, 1177, 1309, 1333
LmnI GCTCC 6 cut(s) 316, 418, 487, 718, 1211, 1229
LweI GCATC 8 cut(s) 355, 529, 823, 832, 990, 1177, 1241, 1278
MaeI CTAG 4 cut(s) 278, 1071, 1160, 1396
MaeII ACGT 1 cut(s) 987
MaeIII GTNAC 5 cut(s) 67, 815, 874, 956, 1300
MalI GATC 7 cut(s) 61, 116, 733, 1022, 1179, 1311, 1335
MboI GATC 7 cut(s) 59, 114, 731, 1020, 1177, 1309, 1333
MboII GAAGA 7 cut(s) 54, 118, 308, 362, 478, 553, 868
MflI RGATCY 1 cut(s) 114
MluCI AATT 3 cut(s) 102, 596, 1085
MlyI GAGTC 2 cut(s) 796, 819
MmeI TCCRAC 2 cut(s) 592, 1207
MseI TTAA 4 cut(s) 180, 495, 696, 861
MslI CAYNNNNRTG 2 cut(s) 1028, 1274
MspA1I CMGCKG 2 cut(s) 479, 1114
MspI CCGG 5 cut(s) 79, 419, 485, 524, 1028
MspR9I CCNGG 3 cut(s) 80, 717, 1203
MvaI CCWGG 2 cut(s) 717, 1203
NciI CCSGG 1 cut(s) 80
NdeII GATC 7 cut(s) 59, 114, 731, 1020, 1177, 1309, 1333
NlaIII CATG 3 cut(s) 133, 292, 1279
NlaIV GGNNCC 4 cut(s) 414, 741, 1141, 1377
NmuCI GTSAC 2 cut(s) 874, 1300
OliI CACNNNNGTG 1 cut(s) 1028
PaeR7I CTCGAG 1 cut(s) 1101
PaqCI CACCTGC 1 cut(s) 1282
PfeI GAWTC 3 cut(s) 93, 230, 404
PleI GAGTC 2 cut(s) 795, 818
PpsI GAGTC 2 cut(s) 795, 818
PpuMI RGGWCCY 2 cut(s) 1375, 1384
Psp1406I AACGTT 1 cut(s) 987
Psp5II RGGWCCY 2 cut(s) 1375, 1384
Psp6I CCWGG 2 cut(s) 715, 1201
PspGI CCWGG 2 cut(s) 715, 1201
PspN4I GGNNCC 4 cut(s) 414, 741, 1141, 1377
PspPI GGNCC 3 cut(s) 620, 1375, 1384
PspPPI RGGWCCY 2 cut(s) 1375, 1384
PspXI VCTCGAGB 1 cut(s) 1101
PstI CTGCAG 2 cut(s) 442, 1119
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 1 cut(s) 114
PvuII CAGCTG 2 cut(s) 479, 1114
RsaI GTAC 3 cut(s) 829, 1130, 1141
RsaNI GTAC 3 cut(s) 828, 1129, 1140
RseI CAYNNNNRTG 2 cut(s) 1028, 1274
SaqAI TTAA 4 cut(s) 180, 495, 696, 861
Sau3AI GATC 7 cut(s) 59, 114, 731, 1020, 1177, 1309, 1333
Sau96I GGNCC 3 cut(s) 620, 1375, 1384
SchI GAGTC 2 cut(s) 796, 819
ScrFI CCNGG 3 cut(s) 80, 717, 1203
SfaNI GCATC 8 cut(s) 355, 529, 823, 832, 990, 1177, 1241, 1278
SfcI CTRYAG 2 cut(s) 438, 1115
Sfr274I CTCGAG 1 cut(s) 1101
SinI GGWCC 3 cut(s) 620, 1375, 1384
SlaI CTCGAG 1 cut(s) 1101
SmiMI CAYNNNNRTG 2 cut(s) 1028, 1274
SmlI CTYRAG 1 cut(s) 1101
SmoI CTYRAG 1 cut(s) 1101
Sse9I AATT 3 cut(s) 102, 596, 1085
SsiI CCGC 5 cut(s) 500, 506, 515, 656, 1267
SspMI CTAG 4 cut(s) 278, 1071, 1160, 1396
StyD4I CCNGG 3 cut(s) 78, 715, 1201
StyI CCWWGG 1 cut(s) 1159
TaaI ACNGT 2 cut(s) 962, 1344
TaiI ACGT 1 cut(s) 990
TaqI TCGA 2 cut(s) 866, 1102
TaqII GACCGA 2 cut(s) 170, 637
TasI AATT 3 cut(s) 102, 596, 1085
TatI WGTACW 1 cut(s) 1128
TauI GCSGC 2 cut(s) 509, 1269
TfiI GAWTC 3 cut(s) 93, 230, 404
Tru1I TTAA 4 cut(s) 180, 495, 696, 861
Tru9I TTAA 4 cut(s) 180, 495, 696, 861
TscAI CASTG 3 cut(s) 921, 1141, 1251
TseFI GTSAC 2 cut(s) 874, 1300
Tsp45I GTSAC 2 cut(s) 874, 1300
TspDTI ATGAA 2 cut(s) 118, 1292
TspGWI ACGGA 2 cut(s) 55, 845
TspRI CASTG 3 cut(s) 921, 1141, 1251
VpaK11BI GGWCC 3 cut(s) 620, 1375, 1384
XagI CCTNNNNNAGG 1 cut(s) 1206
XbaI TCTAGA 1 cut(s) 1070
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XhoI CTCGAG 1 cut(s) 1101
XmaJI CCTAGG 1 cut(s) 1159
XspI CTAG 4 cut(s) 278, 1071, 1160, 1396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.