Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010794

Physical Location & Seq

Genomic Coordinates Forward (+)

6274788 .. 6278780

Gene Structure 3993 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1046900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGGCTCGCCTTCTCAACACCTCCTTCATCCCCAGCTCCCAATCCCTCCGCCGGAACGCCACTTCCTCTACCCCGGCCCTCATTTCCGGGCGCCGGAGCAGGGCCCAAGTCCAGGCCAAGATCCGGGAGATCTTCATGCCGGCTCTCAGCTCCACCATGACTGAGGGCAAGATCGTCTCTTGGGTCAAGTCCGAGGGCGACAAGCTATCCAAGGGCGAGAGCGTCGTCGTCGTCGAGTCCGATAAGGCCGACATGGACGTCGAGACTTTCTACGACGGTTACCTGGCTTCGATTATGGTGGAGGAGGGCGGCGTCGCCACCGTCGGTTCGGCTATTGCTCTATTGGCCGAGACCGAGGACGAGATTGCCGAGGCCAAAGCCAAGGCCGGTTCTTCATCAGCAGCTCCAGTGGCACCAGCACCGGCTGCAGCTGCTCCGGAGCCACTACCGGAGAAAATTCCGGATAATGTGGGTCCTGCGGTGGTCCCTAAGGCGGCGGCTACTGTGGCAGCGGTGGCTTCCACCCACCCGGCATCAGAGGGAGGGAAGAGAATCGTGGCATCGCCGTATGCGAAGAAGCTTGCAAAGGACTTGAAGGTGGATTTGGGGTCAGTGGTGGGAAGTGGACCACTGGGGAGGATTGTGGCCAAGGATGTTGAAGCTTTTGCTGCTCAGCCGACGGTGGCGGCTGTTGCTGAACCGAGTGTTGGGAAGTCAGCTGCTGCGGTGGCGGCGGAGGCGGGAATTCAGTTGGGATCGGTGGTGCCTTTTACGACAATGCAGGGGGCTGTGAGTAGAAACATGCTTGAGAGTCTCTCAGTGCCAACTTTCAGAGTAGGATACACTATCACCACTGATGCGCTCGATGCTCTGTACAAAAAGATCAAGTCGAAGGGAGTTACCATGACAGCATTGCTTGCAAAGGCTACAGCACTTGCATTGGTCAAACATCCTGTTGTCAACTCCGGCTGTAGAGATGGTAAGAGCTTTACATACAACAGCAACATTAACATTGCAGTCGCTGTGGCAATCGATGGTGGATTGATTACACCAGTGCTTCAGGATGCGGACAAGGTTGACATATATACATTGTCGAGAAAGTGGAAGGAGTTAGTTGATAAGGCCAGGGCCAAGCAGCTACAACCTCACGAGTACAATACAGGTACTTTCACTGTTTCTAATCTTGGGATGTTTGGTGTTGATCGGTTTGATGCTATTCTGCCGCCTGGAACTGGAGCAATTATGGCTGTTGGATCATCTCAGCCTACTGTTGTGGCTACTAAAGATGGTCGGATTGGACGGAAGAACCAAATGCAGGTAAATGTTACTGCTGATCATCGTATAATTTATGGCGCTGATCTGGCTTCATTCTTGCAAACCTTGGCCAAGATTATTGAGGACCCCAAAGATCTTACCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005886 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016020 GO:0019904 GO:0022626 GO:0031967 GO:0031975 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0071944 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

474

Amino Acids

49.28

Weight (kDa)

8.82

Isoelectric Point (pI)

37.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	43 - 114	1.7e-15	Biotin-requiring enzyme
E3_binding	PF02817	185 - 220	2.1e-13	e3 binding domain
2-oxoacid_dh	PF00198	254 - 471	1.5e-63	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010794)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34430
fragaria_vesca	FvH4_2g12130
malus_domestica	MD05G1040900.v1.1 MD10G1046900.v1.1
prunus_persica	Prupe.8G056000_v2.0.a1
pyrus_communis	pycom05g03360 pycom10g03320
rosa_chinensis	RchiOBHm_Chr6g0272101 RchiOBHm_Chr6g0272111
rosa_laevigata	RLG00000013702
rosa_multiflora	Rmu_sc0013301.1_g000004 Rmu_sc0032599.1_g000001
rosa_roxburghii	Rroxscaffold_7G00196600
rosa_rugosa	Rorug06G0065600
rosa_samantha	Rh6AG182900 Rh6BG184200 Rh6CG184100 Rh6DG175700
rosa_wichuraiana	Rw0G009140 Rw6G015740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	257
AatII	GACGTC	1 cut(s)	261
Acc36I	ACCTGC	1 cut(s)	1306
AccB1I	GGYRCC	3 cut(s)	90, 412, 763
AccIII	TCCGGA	2 cut(s)	436, 460
AclWI	GGATC	3 cut(s)	115, 763, 1261
AcoI	YGGCCR	3 cut(s)	345, 645, 1383
AcsI	RAATTY	2 cut(s)	456, 744
AcuI	CTGAAG	1 cut(s)	1043
AcyI	GRCGYC	3 cut(s)	91, 258, 312
AfaI	GTAC	3 cut(s)	875, 1154, 1165
AfiI	CCNNNNNNNGG	9 cut(s)	51, 93, 100, 112, 123, 493, 707, 1232, 1315
AgsI	TTSAA	2 cut(s)	595, 659
AjnI	CCWGG	4 cut(s)	111, 282, 1124, 1225
AjuI	GAANNNNNNNTTGG	4 cut(s)	587, 619, 690, 722
AloI	GAACNNNNNNTCC	2 cut(s)	47, 79
Alw26I	GTCTC	4 cut(s)	181, 257, 344, 818
AlwI	GGATC	3 cut(s)	115, 763, 1261
AlwNI	CAGNNNCTG	2 cut(s)	722, 1022
Aor13HI	TCCGGA	2 cut(s)	436, 460
ApaI	GGGCCC	1 cut(s)	106
ApeKI	GCWGC	9 cut(s)	401, 425, 428, 431, 509, 668, 719, 722, 1135
ApoI	RAATTY	2 cut(s)	456, 744
AspLEI	GCGC	3 cut(s)	93, 862, 1355
AspS9I	GGNCC	8 cut(s)	76, 102, 103, 473, 484, 626, 1128, 1399
AsuC2I	CCSGG	4 cut(s)	74, 88, 125, 530
AsuHPI	GGTGA	1 cut(s)	841
AvaII	GGWCC	4 cut(s)	473, 484, 626, 1399
AxyI	CCTNAGG	1 cut(s)	489
BaeGI	GKGCMC	1 cut(s)	106
BalI	TGGCCA	2 cut(s)	647, 1385
BanI	GGYRCC	3 cut(s)	90, 412, 763
BanII	GRGCYC	1 cut(s)	106
BauI	CACGAG	1 cut(s)	1148
BbvI	GCAGC	9 cut(s)	412, 413, 418, 440, 521, 655, 706, 709, 1147
BccI	CCATC	3 cut(s)	971, 1028, 1280
BceAI	ACGGC	1 cut(s)	550
BciT130I	CCWGG	4 cut(s)	113, 284, 1126, 1227
BciVI	GTATCC	1 cut(s)	833
BclI	TGATCA	1 cut(s)	1333
BcnI	CCSGG	4 cut(s)	74, 88, 125, 530
BcoDI	GTCTC	4 cut(s)	181, 257, 344, 818
BfaI	CTAG	1 cut(s)	1420
BfmI	CTRYAG	3 cut(s)	426, 927, 970
BfoI	RGCGCY	2 cut(s)	94, 1356
BfuAI	ACCTGC	1 cut(s)	1306
BfuI	GTATCC	1 cut(s)	833
BglII	AGATCT	2 cut(s)	129, 1408
BlpI	GCTNAGC	1 cut(s)	672
Bme1390I	CCNGG	8 cut(s)	74, 88, 113, 125, 284, 530, 1126, 1227
Bme18I	GGWCC	4 cut(s)	473, 484, 626, 1399
BmgT120I	GGNCC	8 cut(s)	76, 102, 103, 473, 484, 626, 1128, 1399
BmiI	GGNNCC	8 cut(s)	92, 104, 414, 441, 474, 486, 765, 1401
BmrFI	CCNGG	8 cut(s)	74, 88, 113, 125, 284, 530, 1126, 1227
BmrI	ACTGGG	1 cut(s)	641
BmsI	GCATC	6 cut(s)	542, 569, 847, 856, 1054, 1201
BmuI	ACTGGG	1 cut(s)	641
BplI	GAGNNNNNCTC	2 cut(s)	800, 832
BpmI	CTGGAG	2 cut(s)	390, 1254
Bpu1102I	GCTNAGC	1 cut(s)	672
BpuEI	CTTGAG	1 cut(s)	827
BpuMI	CCSGG	4 cut(s)	74, 88, 125, 530
Bsa29I	ATCGAT	1 cut(s)	1032
BsaHI	GRCGYC	3 cut(s)	91, 258, 312
BsaI	GGTCTC	1 cut(s)	344
BsaJI	CCNNGG	9 cut(s)	72, 192, 210, 354, 369, 381, 648, 1125, 1380
BsaWI	WCCGGW	3 cut(s)	436, 448, 460
Bsc4I	CCNNNNNNNGG	9 cut(s)	51, 93, 100, 112, 123, 493, 707, 1232, 1315
Bse118I	RCCGGY	3 cut(s)	139, 386, 421
Bse1I	ACTGG	4 cut(s)	407, 636, 1052, 1237
Bse21I	CCTNAGG	1 cut(s)	489
Bse3DI	GCAATG	2 cut(s)	911, 1011
BseAI	TCCGGA	2 cut(s)	436, 460
BseBI	CCWGG	4 cut(s)	113, 284, 1126, 1227
BseCI	ATCGAT	1 cut(s)	1032
BseDI	CCNNGG	9 cut(s)	72, 192, 210, 354, 369, 381, 648, 1125, 1380
BseGI	GGATG	5 cut(s)	27, 658, 949, 1069, 1194
BseLI	CCNNNNNNNGG	9 cut(s)	51, 93, 100, 112, 123, 493, 707, 1232, 1315
BseMI	GCAATG	2 cut(s)	911, 1011
BseMII	CTCAG	5 cut(s)	153, 160, 686, 831, 1274
BseNI	ACTGG	4 cut(s)	407, 636, 1052, 1237
BseRI	GAGGAG	1 cut(s)	317
BseSI	GKGCMC	1 cut(s)	106
BseXI	GCAGC	9 cut(s)	412, 413, 418, 440, 521, 655, 706, 709, 1147
BseYI	CCCAGC	1 cut(s)	32
BshNI	GGYRCC	3 cut(s)	90, 412, 763
BshVI	ATCGAT	1 cut(s)	1032
BslFI	GGGAC	1 cut(s)	470
BslI	CCNNNNNNNGG	9 cut(s)	51, 93, 100, 112, 123, 493, 707, 1232, 1315
BsmAI	GTCTC	4 cut(s)	181, 257, 344, 818
BsmBI	CGTCTC	1 cut(s)	181
BsmFI	GGGAC	1 cut(s)	470
Bso31I	GGTCTC	1 cut(s)	344
Bsp120I	GGGCCC	1 cut(s)	102
Bsp1286I	GDGCHC	1 cut(s)	106
Bsp13I	TCCGGA	2 cut(s)	436, 460
Bsp1407I	TGTACA	1 cut(s)	873
Bsp1720I	GCTNAGC	1 cut(s)	672
BspCNI	CTCAG	5 cut(s)	154, 159, 685, 830, 1273
BspDI	ATCGAT	1 cut(s)	1032
BspEI	TCCGGA	2 cut(s)	436, 460
BspLI	GGNNCC	8 cut(s)	92, 104, 414, 441, 474, 486, 765, 1401
BspMAI	CTGCAG	1 cut(s)	430
BspMI	ACCTGC	1 cut(s)	1306
BspPI	GGATC	3 cut(s)	115, 763, 1261
BspT107I	GGYRCC	3 cut(s)	90, 412, 763
BspTNI	GGTCTC	1 cut(s)	344
BsrDI	GCAATG	2 cut(s)	911, 1011
BsrFI	RCCGGY	3 cut(s)	139, 386, 421
BsrGI	TGTACA	1 cut(s)	873
BsrI	ACTGG	4 cut(s)	407, 636, 1052, 1237
BssAI	RCCGGY	3 cut(s)	139, 386, 421
BssECI	CCNNGG	9 cut(s)	72, 192, 210, 354, 369, 381, 648, 1125, 1380
BssNI	GRCGYC	3 cut(s)	91, 258, 312
BssSI	CACGAG	1 cut(s)	1148
BssT1I	CCWWGG	4 cut(s)	210, 381, 648, 1380
Bst2BI	CACGAG	1 cut(s)	1148
Bst2UI	CCWGG	4 cut(s)	113, 284, 1126, 1227
Bst4CI	ACNGT	6 cut(s)	278, 322, 505, 682, 1174, 1270
Bst6I	CTCTTC	1 cut(s)	542
BstACI	GRCGYC	3 cut(s)	91, 258, 312
BstAPI	GCANNNNNTGC	2 cut(s)	425, 917
BstAUI	TGTACA	1 cut(s)	873
BstC8I	GCNNGC	4 cut(s)	7, 141, 582, 918
BstDEI	CTNAG	6 cut(s)	146, 162, 489, 672, 817, 1260
BstEII	GGTNACC	1 cut(s)	278
BstF5I	GGATG	5 cut(s)	27, 658, 949, 1069, 1194
BstH2I	RGCGCY	2 cut(s)	94, 1356
BstHHI	GCGC	3 cut(s)	93, 862, 1355
BstMAI	GTCTC	4 cut(s)	181, 257, 344, 818
BstNI	CCWGG	4 cut(s)	113, 284, 1126, 1227
BstNSI	RCATGY	1 cut(s)	805
BstPI	GGTNACC	1 cut(s)	278
BstSCI	CCNGG	8 cut(s)	72, 86, 111, 123, 282, 528, 1124, 1225
BstSFI	CTRYAG	3 cut(s)	426, 927, 970
BstSLI	GKGCMC	1 cut(s)	106
BstV1I	GCAGC	9 cut(s)	412, 413, 418, 440, 521, 655, 706, 709, 1147
BstX2I	RGATCY	3 cut(s)	120, 129, 1408
BstYI	RGATCY	3 cut(s)	120, 129, 1408
Bsu15I	ATCGAT	1 cut(s)	1032
Bsu36I	CCTNAGG	1 cut(s)	489
BsuI	GTATCC	1 cut(s)	833
BsuTUI	ATCGAT	1 cut(s)	1032
BtgZI	GCGATG	1 cut(s)	546
BtsCI	GGATG	5 cut(s)	27, 658, 949, 1069, 1194
BtsIMutI	CAGTG	7 cut(s)	414, 618, 629, 825, 852, 1059, 1170
BveI	ACCTGC	1 cut(s)	1306
Cac8I	GCNNGC	4 cut(s)	7, 141, 582, 918
CaiI	CAGNNNCTG	2 cut(s)	722, 1022
CfoI	GCGC	3 cut(s)	93, 862, 1355
Cfr10I	RCCGGY	3 cut(s)	139, 386, 421
Cfr13I	GGNCC	8 cut(s)	76, 102, 103, 473, 484, 626, 1128, 1399
ClaI	ATCGAT	1 cut(s)	1032
CseI	GACGC	2 cut(s)	211, 301
Csp6I	GTAC	3 cut(s)	874, 1153, 1164
CviAII	CATG	5 cut(s)	136, 157, 253, 802, 904
CviQI	GTAC	3 cut(s)	874, 1153, 1164
DdeI	CTNAG	6 cut(s)	146, 162, 489, 672, 817, 1260
DinI	GGCGCC	1 cut(s)	92
DrdI	GACNNNNNNGTC	1 cut(s)	257
DseDI	GACNNNNNNGTC	1 cut(s)	257
EaeI	YGGCCR	3 cut(s)	345, 645, 1383
Eam1104I	CTCTTC	1 cut(s)	542
EarI	CTCTTC	1 cut(s)	542
EciI	GGCGGA	2 cut(s)	38, 749
Eco130I	CCWWGG	4 cut(s)	210, 381, 648, 1380
Eco24I	GRGCYC	1 cut(s)	106
Eco31I	GGTCTC	1 cut(s)	344
Eco47I	GGWCC	4 cut(s)	473, 484, 626, 1399
Eco57I	CTGAAG	1 cut(s)	1043
Eco81I	CCTNAGG	1 cut(s)	489
Eco91I	GGTNACC	1 cut(s)	278
EcoO109I	RGGNCCY	3 cut(s)	102, 473, 1399
EcoO65I	GGTNACC	1 cut(s)	278
EcoRI	GAATTC	1 cut(s)	744
EcoRII	CCWGG	4 cut(s)	111, 282, 1124, 1225
EcoT14I	CCWWGG	4 cut(s)	210, 381, 648, 1380
EcoT38I	GRGCYC	1 cut(s)	106
EgeI	GGCGCC	1 cut(s)	92
EheI	GGCGCC	1 cut(s)	92
ErhI	CCWWGG	4 cut(s)	210, 381, 648, 1380
Esp3I	CGTCTC	1 cut(s)	181
FaeI	CATG	5 cut(s)	139, 160, 256, 805, 907
FaqI	GGGAC	1 cut(s)	470
FatI	CATG	5 cut(s)	135, 156, 252, 801, 903
FauI	CCCGC	1 cut(s)	733
FbaI	TGATCA	1 cut(s)	1333
FokI	GGATG	5 cut(s)	14, 665, 936, 1076, 1201
FriOI	GRGCYC	1 cut(s)	106
FspBI	CTAG	1 cut(s)	1420
GlaI	GCGC	3 cut(s)	92, 861, 1354
GsaI	CCCAGC	1 cut(s)	36
GsuI	CTGGAG	2 cut(s)	390, 1254
HaeII	RGCGCY	2 cut(s)	94, 1356
HgaI	GACGC	2 cut(s)	211, 301
HhaI	GCGC	3 cut(s)	93, 862, 1355
Hin1I	GRCGYC	3 cut(s)	91, 258, 312
Hin1II	CATG	5 cut(s)	139, 160, 256, 805, 907
Hin6I	GCGC	3 cut(s)	91, 860, 1353
HinP1I	GCGC	3 cut(s)	91, 860, 1353
HincII	GTYRAC	2 cut(s)	961, 1078
HindII	GTYRAC	2 cut(s)	961, 1078
HindIII	AAGCTT	2 cut(s)	578, 660
HinfI	GANTC	3 cut(s)	236, 552, 811
HphI	GGTGA	1 cut(s)	841
Hpy166II	GTNNAC	3 cut(s)	626, 961, 1078
Hpy188I	TCNGA	5 cut(s)	193, 241, 538, 833, 1293
Hpy188III	TCNNGA	6 cut(s)	262, 437, 461, 1061, 1095, 1148
Hpy8I	GTNNAC	3 cut(s)	626, 961, 1078
Hpy99I	CGWCG	9 cut(s)	227, 230, 233, 236, 263, 278, 317, 326, 682
HpyAV	CCTTC	5 cut(s)	20, 34, 589, 886, 1099
HpyCH4III	ACNGT	6 cut(s)	278, 322, 505, 682, 1174, 1270
HpyCH4IV	ACGT	1 cut(s)	258
HpyCH4V	TGCA	8 cut(s)	428, 584, 781, 920, 938, 1016, 1315, 1375
HpyF3I	CTNAG	6 cut(s)	146, 162, 489, 672, 817, 1260
HpySE526I	ACGT	1 cut(s)	258
Hsp92I	GRCGYC	3 cut(s)	91, 258, 312
Hsp92II	CATG	5 cut(s)	139, 160, 256, 805, 907
HspAI	GCGC	3 cut(s)	91, 860, 1353
KasI	GGCGCC	1 cut(s)	90
Kpn2I	TCCGGA	2 cut(s)	436, 460
KroI	GCCGGC	1 cut(s)	139
KroNI	GCCGGC	1 cut(s)	141
Ksp22I	TGATCA	1 cut(s)	1333
LmnI	GCTCC	7 cut(s)	41, 96, 155, 409, 439, 439, 1235
Lsp1109I	GCAGC	9 cut(s)	412, 413, 418, 440, 521, 655, 706, 709, 1147
LweI	GCATC	6 cut(s)	542, 569, 847, 856, 1054, 1201
MaeI	CTAG	1 cut(s)	1420
MaeII	ACGT	1 cut(s)	258
MaeIII	GTNAC	3 cut(s)	278, 898, 1324
MboII	GAAGA	5 cut(s)	124, 384, 559, 586, 1315
MflI	RGATCY	3 cut(s)	120, 129, 1408
MhlI	GDGCHC	1 cut(s)	106
MlsI	TGGCCA	2 cut(s)	647, 1385
MluCI	AATT	4 cut(s)	456, 744, 1239, 1344
MluNI	TGGCCA	2 cut(s)	647, 1385
Mly113I	GGCGCC	1 cut(s)	91
MlyI	GAGTC	2 cut(s)	245, 820
MmeI	TCCRAC	2 cut(s)	1231, 1271
Mox20I	TGGCCA	2 cut(s)	647, 1385
MroI	TCCGGA	2 cut(s)	436, 460
MroNI	GCCGGC	1 cut(s)	139
MscI	TGGCCA	2 cut(s)	647, 1385
MseI	TTAA	1 cut(s)	1008
Msp20I	TGGCCA	2 cut(s)	647, 1385
MspA1I	CMGCKG	3 cut(s)	431, 512, 719
MspR9I	CCNGG	8 cut(s)	74, 88, 113, 125, 284, 530, 1126, 1227
MvaI	CCWGG	4 cut(s)	113, 284, 1126, 1227
NaeI	GCCGGC	1 cut(s)	141
NarI	GGCGCC	1 cut(s)	91
NciI	CCSGG	4 cut(s)	74, 88, 125, 530
NgoMIV	GCCGGC	1 cut(s)	139
NlaIII	CATG	5 cut(s)	139, 160, 256, 805, 907
NlaIV	GGNNCC	8 cut(s)	92, 104, 414, 441, 474, 486, 765, 1401
NmeAIII	GCCGAG	2 cut(s)	373, 394
NspI	RCATGY	1 cut(s)	805
PcsI	WCGNNNNNNNCGW	4 cut(s)	231, 237, 366, 569
PdiI	GCCGGC	1 cut(s)	141
PfeI	GAWTC	1 cut(s)	552
PfoI	TCCNGGA	1 cut(s)	123
PleI	GAGTC	2 cut(s)	244, 819
PluTI	GGCGCC	1 cut(s)	94
PpsI	GAGTC	2 cut(s)	244, 819
PpuMI	RGGWCCY	2 cut(s)	473, 1399
Psp5II	RGGWCCY	2 cut(s)	473, 1399
Psp6I	CCWGG	4 cut(s)	111, 282, 1124, 1225
PspEI	GGTNACC	1 cut(s)	278
PspFI	CCCAGC	1 cut(s)	32
PspGI	CCWGG	4 cut(s)	111, 282, 1124, 1225
PspN4I	GGNNCC	8 cut(s)	92, 104, 414, 441, 474, 486, 765, 1401
PspOMI	GGGCCC	1 cut(s)	102
PspPI	GGNCC	8 cut(s)	76, 102, 103, 473, 484, 626, 1128, 1399
PspPPI	RGGWCCY	2 cut(s)	473, 1399
PstI	CTGCAG	1 cut(s)	430
PstNI	CAGNNNCTG	2 cut(s)	722, 1022
PsuI	RGATCY	3 cut(s)	120, 129, 1408
PvuII	CAGCTG	2 cut(s)	431, 719
RsaI	GTAC	3 cut(s)	875, 1154, 1165
RsaNI	GTAC	3 cut(s)	874, 1153, 1164
SaqAI	TTAA	1 cut(s)	1008
Sau96I	GGNCC	8 cut(s)	76, 102, 103, 473, 484, 626, 1128, 1399
SchI	GAGTC	2 cut(s)	245, 820
ScrFI	CCNGG	8 cut(s)	74, 88, 113, 125, 284, 530, 1126, 1227
SduI	GDGCHC	1 cut(s)	106
SfaNI	GCATC	6 cut(s)	542, 569, 847, 856, 1054, 1201
SfcI	CTRYAG	3 cut(s)	426, 927, 970
SfoI	GGCGCC	1 cut(s)	92
SinI	GGWCC	4 cut(s)	473, 484, 626, 1399
SmlI	CTYRAG	1 cut(s)	806
SmoI	CTYRAG	1 cut(s)	806
Sse9I	AATT	4 cut(s)	456, 744, 1239, 1344
SspDI	GGCGCC	1 cut(s)	90
SspMI	CTAG	1 cut(s)	1420
StyD4I	CCNGG	8 cut(s)	72, 86, 111, 123, 282, 528, 1124, 1225
StyI	CCWWGG	4 cut(s)	210, 381, 648, 1380
TaaI	ACNGT	6 cut(s)	278, 322, 505, 682, 1174, 1270
TaiI	ACGT	1 cut(s)	261
TaqI	TCGA	7 cut(s)	234, 261, 290, 864, 890, 1032, 1094
TaqII	GACCGA	1 cut(s)	368
TasI	AATT	4 cut(s)	456, 744, 1239, 1344
TatI	WGTACW	2 cut(s)	873, 1152
TauI	GCSGC	6 cut(s)	312, 497, 500, 689, 734, 1225
TfiI	GAWTC	1 cut(s)	552
Tru1I	TTAA	1 cut(s)	1008
Tru9I	TTAA	1 cut(s)	1008
TscAI	CASTG	7 cut(s)	414, 618, 636, 825, 859, 1059, 1177
TseI	GCWGC	9 cut(s)	401, 425, 428, 431, 509, 668, 719, 722, 1135
TspDTI	ATGAA	4 cut(s)	16, 124, 384, 1356
TspGWI	ACGGA	1 cut(s)	1315
TspRI	CASTG	7 cut(s)	414, 618, 636, 825, 859, 1059, 1177
VpaK11BI	GGWCC	4 cut(s)	473, 484, 626, 1399
XapI	RAATTY	2 cut(s)	456, 744
XceI	RCATGY	1 cut(s)	805
XspI	CTAG	1 cut(s)	1420
ZraI	GACGTC	1 cut(s)	259

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.