Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010794

Physical Location & Seq

Genomic Coordinates Forward (+)

6919253 .. 6923534

Gene Structure 4282 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1040900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGCTCACCTTCTCAACACCTCCTTCATCCCCACCTCCCAACCCCTCCGCCGTAACCCCACCTCCTCTGCGCCGGCACTCATTTCCGGGCGCCGGGGCAGGGCCCAAGTCCAGGCCAAGATCCGGGAGATCTTCATGCCGGCTCTCAGCTCCACCATGACTGAGGGCAAGATCGTCTCCTGGGTCAAGTCCGAGGGCGACAAGCTCTCCAAGGGCGAGAGCGTCGTCGTCGTCGAGTCCGATAAGGCTGACATGGACGTCGAGACCTTCTACGACGGTTACTTGGCTTCTATTATGGTCGAGGAGGGCGGCGTCGCAGCCGTCGGTTCGGCTATTGCTCTATTGGCCGAAACCGAGGACGAGATTGCCGAGGCCAAGACCAAGGCCAATTCTTCATCAGCAGCTCCAGCTCCAGCGGCACCAGCACCGCCCGCAACTGCTCCGGAGCCACAGCCGGAGAAAATTCCAGATAATGCGGGTCCCGCGGTGGTCCCTAAGGCTGCGGCAACCGTGGCTTCAACCCACCCGGCGTCAGAGGGAGGGAAGAGAATCGTGGCATCGCCGTATGCAAAGAAGCTTGCTAAGGACTTGAAGGTGGACTTGGGGTCAGTGGTGGGAAGTGGACCGCTGGGGAGGATTGTGGCTAAGGATGTTGAAGCTTTTGCTACTCAGCCGACGGTGGCGGTGGCTGCTGAACCGAGTGTTGGAAAGTCAGCCGCTGCTGTGGCAGCGGAGGCAGGAATTGAGTTGGGATCGGTGGTGCCTTTTACAACAATGCAGGGGGCAGTGAGTAGGAACATGCTTGAGAGTCTCTCTGTGCCCACTTTCAGAGTAGGATACACTATCACCACTGACGCGCTGGATGCCCTGTACAAAAAGATCAAGTCGAAGGGAGTGACCATGACAGCATTGCTTGCAAAGGCTACTGCACTTGCATTGGTCAAACATCCTGTTGTGAACTCTGGCTGTAGAGATGGTAAGAGCTTTACATACAACAGCAGCATTAACATAGCGGTTGCTGTGGCAATCGACGGTGGATTGATTACGCCAGTGCTTCAGGATGCGGACAAGGTTGACATATATACATTGTCAAGAAAGTGGAAGGAGTTAGTTGATAAGGCCAGGGCCAAGCAGCTACAACCTCATGAGTACAATACAGGTACATTCACTGTTTCTAATCTTGGGATGTTTGGTGTTGATCGGTTTGATGCTATTCTGCCACCTGGAACTGGTGCAATTATGGCTGTCGGATCATCTCAGCCAACTGTTGTGGCTACTAAGGATGGTCGTATTGGTAGGAAGAACCAAATGCAGGTAAATGTTACTGCTGACCACCGCATAATTTATGGAGCTGATCTTGCTTCATTCTTGCAAACCTTGGCCAAGATTATTGAGGACCCCAAAGATCTTACCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005886 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016020 GO:0019904 GO:0022626 GO:0031967 GO:0031975 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0071944 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

473

Amino Acids

49.25

Weight (kDa)

8.33

Isoelectric Point (pI)

36.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	43 - 114	2.8e-15	Biotin-requiring enzyme
E3_binding	PF02817	184 - 219	2.1e-13	e3 binding domain
2-oxoacid_dh	PF00198	253 - 470	1.9e-63	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010794)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34430
fragaria_vesca	FvH4_2g12130
malus_domestica	MD05G1040900.v1.1 MD10G1046900.v1.1
prunus_persica	Prupe.8G056000_v2.0.a1
pyrus_communis	pycom05g03360 pycom10g03320
rosa_chinensis	RchiOBHm_Chr6g0272101 RchiOBHm_Chr6g0272111
rosa_laevigata	RLG00000013702
rosa_multiflora	Rmu_sc0013301.1_g000004 Rmu_sc0032599.1_g000001
rosa_roxburghii	Rroxscaffold_7G00196600
rosa_rugosa	Rorug06G0065600
rosa_samantha	Rh6AG182900 Rh6BG184200 Rh6CG184100 Rh6DG175700
rosa_wichuraiana	Rw0G009140 Rw6G015740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	257
AatII	GACGTC	1 cut(s)	261
Acc36I	ACCTGC	1 cut(s)	1303
AccB1I	GGYRCC	3 cut(s)	90, 418, 760
AccII	CGCG	2 cut(s)	485, 857
AccIII	TCCGGA	1 cut(s)	442
AclWI	GGATC	3 cut(s)	115, 760, 1258
AcoI	YGGCCR	2 cut(s)	345, 1380
AcsI	RAATTY	1 cut(s)	462
AcuI	CTGAAG	1 cut(s)	1040
AcyI	GRCGYC	4 cut(s)	91, 258, 312, 530
AfaI	GTAC	3 cut(s)	872, 1151, 1162
AfiI	CCNNNNNNNGG	7 cut(s)	93, 100, 112, 123, 704, 1229, 1312
AgsI	TTSAA	3 cut(s)	519, 592, 656
AhdI	GACNNNNNGTC	1 cut(s)	604
AjnI	CCWGG	4 cut(s)	111, 179, 1121, 1222
AjuI	GAANNNNNNNTTGG	4 cut(s)	584, 616, 687, 719
AluBI	AGCT	9 cut(s)	150, 205, 404, 410, 577, 659, 984, 1135, 1352
AluI	AGCT	9 cut(s)	150, 205, 404, 410, 577, 659, 984, 1135, 1352
Alw26I	GTCTC	3 cut(s)	181, 257, 815
AlwI	GGATC	3 cut(s)	115, 760, 1258
AlwNI	CAGNNNCTG	1 cut(s)	719
Aor13HI	TCCGGA	1 cut(s)	442
AoxI	GGCC	8 cut(s)	102, 114, 345, 372, 384, 1119, 1125, 1380
ApaI	GGGCCC	1 cut(s)	106
ApeKI	GCWGC	8 cut(s)	317, 401, 500, 689, 719, 728, 999, 1132
ApoI	RAATTY	1 cut(s)	462
AspLEI	GCGC	3 cut(s)	73, 93, 859
AspS9I	GGNCC	7 cut(s)	102, 103, 479, 490, 623, 1125, 1396
AsuC2I	CCSGG	4 cut(s)	88, 95, 125, 527
AsuHPI	GGTGA	1 cut(s)	838
AvaII	GGWCC	4 cut(s)	479, 490, 623, 1396
AxyI	CCTNAGG	1 cut(s)	495
BaeGI	GKGCMC	2 cut(s)	106, 822
BalI	TGGCCA	1 cut(s)	1382
BanI	GGYRCC	3 cut(s)	90, 418, 760
BanII	GRGCYC	1 cut(s)	106
BbvI	GCAGC	8 cut(s)	329, 413, 487, 676, 706, 740, 1011, 1144
BccI	CCATC	2 cut(s)	968, 1277
BceAI	ACGGC	3 cut(s)	36, 305, 547
BciT130I	CCWGG	4 cut(s)	113, 181, 1123, 1224
BciVI	GTATCC	1 cut(s)	830
BcnI	CCSGG	4 cut(s)	88, 95, 125, 527
BcoDI	GTCTC	3 cut(s)	181, 257, 815
BfaI	CTAG	1 cut(s)	1417
BfmI	CTRYAG	1 cut(s)	967
BfoI	RGCGCY	1 cut(s)	94
BfuAI	ACCTGC	1 cut(s)	1303
BfuI	GTATCC	1 cut(s)	830
BglII	AGATCT	2 cut(s)	129, 1405
Bme1390I	CCNGG	8 cut(s)	88, 95, 113, 125, 181, 527, 1123, 1224
Bme18I	GGWCC	4 cut(s)	479, 490, 623, 1396
BmeRI	GACNNNNNGTC	1 cut(s)	604
BmgT120I	GGNCC	7 cut(s)	102, 103, 479, 490, 623, 1125, 1396
BmiI	GGNNCC	9 cut(s)	92, 104, 420, 447, 480, 481, 492, 762, 1398
BmrFI	CCNGG	8 cut(s)	88, 95, 113, 125, 181, 527, 1123, 1224
BmsI	GCATC	4 cut(s)	566, 853, 1051, 1198
BplI	GAGNNNNNCTC	2 cut(s)	797, 829
BpmI	CTGGAG	2 cut(s)	390, 396
Bpu10I	CCTNAGC	2 cut(s)	582, 645
BpuEI	CTTGAG	1 cut(s)	824
BpuMI	CCSGG	4 cut(s)	88, 95, 125, 527
BsaHI	GRCGYC	4 cut(s)	91, 258, 312, 530
BsaI	GGTCTC	1 cut(s)	257
BsaWI	WCCGGW	1 cut(s)	442
BsaXI	ACNNNNNCTCC	4 cut(s)	47, 77, 191, 221
Bsc4I	CCNNNNNNNGG	7 cut(s)	93, 100, 112, 123, 704, 1229, 1312
Bse118I	RCCGGY	2 cut(s)	73, 139
Bse1I	ACTGG	2 cut(s)	1049, 1234
Bse21I	CCTNAGG	1 cut(s)	495
Bse3DI	GCAATG	1 cut(s)	908
BseAI	TCCGGA	1 cut(s)	442
BseBI	CCWGG	4 cut(s)	113, 181, 1123, 1224
BseGI	GGATG	7 cut(s)	27, 655, 868, 946, 1066, 1191, 1288
BseLI	CCNNNNNNNGG	7 cut(s)	93, 100, 112, 123, 704, 1229, 1312
BseMI	GCAATG	1 cut(s)	908
BseMII	CTCAG	4 cut(s)	153, 160, 683, 1271
BseNI	ACTGG	2 cut(s)	1049, 1234
BseRI	GAGGAG	2 cut(s)	55, 317
BseSI	GKGCMC	2 cut(s)	106, 822
BseXI	GCAGC	8 cut(s)	329, 413, 487, 676, 706, 740, 1011, 1144
BseYI	CCCAGC	1 cut(s)	628
BsgI	GTGCAG	1 cut(s)	912
Bsh1236I	CGCG	2 cut(s)	485, 857
BshFI	GGCC	8 cut(s)	104, 116, 347, 374, 386, 1121, 1127, 1382
BshNI	GGYRCC	3 cut(s)	90, 418, 760
BsiSI	CCGG	8 cut(s)	74, 87, 94, 124, 140, 443, 455, 527
BslFI	GGGAC	2 cut(s)	465, 476
BslI	CCNNNNNNNGG	7 cut(s)	93, 100, 112, 123, 704, 1229, 1312
BsmAI	GTCTC	3 cut(s)	181, 257, 815
BsmBI	CGTCTC	1 cut(s)	181
BsmFI	GGGAC	2 cut(s)	465, 476
BsnI	GGCC	8 cut(s)	104, 116, 347, 374, 386, 1121, 1127, 1382
Bso31I	GGTCTC	1 cut(s)	257
Bsp120I	GGGCCC	1 cut(s)	102
Bsp1286I	GDGCHC	2 cut(s)	106, 822
Bsp13I	TCCGGA	1 cut(s)	442
Bsp1407I	TGTACA	1 cut(s)	870
Bsp143I	GATC	9 cut(s)	120, 129, 171, 752, 879, 1198, 1250, 1354, 1405
BspANI	GGCC	8 cut(s)	104, 116, 347, 374, 386, 1121, 1127, 1382
BspCNI	CTCAG	4 cut(s)	154, 159, 682, 1270
BspEI	TCCGGA	1 cut(s)	442
BspFNI	CGCG	2 cut(s)	485, 857
BspHI	TCATGA	1 cut(s)	1144
BspLI	GGNNCC	9 cut(s)	92, 104, 420, 447, 480, 481, 492, 762, 1398
BspMI	ACCTGC	1 cut(s)	1303
BspPI	GGATC	3 cut(s)	115, 760, 1258
BspT107I	GGYRCC	3 cut(s)	90, 418, 760
BspTNI	GGTCTC	1 cut(s)	257
BsrDI	GCAATG	1 cut(s)	908
BsrFI	RCCGGY	2 cut(s)	73, 139
BsrGI	TGTACA	1 cut(s)	870
BsrI	ACTGG	2 cut(s)	1049, 1234
BssAI	RCCGGY	2 cut(s)	73, 139
BssMI	GATC	9 cut(s)	120, 129, 171, 752, 879, 1198, 1250, 1354, 1405
BssNI	GRCGYC	4 cut(s)	91, 258, 312, 530
BssT1I	CCWWGG	3 cut(s)	210, 381, 1377
Bst2UI	CCWGG	4 cut(s)	113, 181, 1123, 1224
Bst4CI	ACNGT	6 cut(s)	278, 511, 679, 1034, 1171, 1267
Bst6I	CTCTTC	1 cut(s)	539
BstACI	GRCGYC	4 cut(s)	91, 258, 312, 530
BstAPI	GCANNNNNTGC	1 cut(s)	914
BstAUI	TGTACA	1 cut(s)	870
BstC8I	GCNNGC	5 cut(s)	75, 141, 432, 579, 915
BstDEI	CTNAG	8 cut(s)	146, 162, 495, 582, 645, 669, 1257, 1278
BstDSI	CCRYGG	2 cut(s)	483, 510
BstF5I	GGATG	7 cut(s)	27, 655, 868, 946, 1066, 1191, 1288
BstFNI	CGCG	2 cut(s)	485, 857
BstH2I	RGCGCY	1 cut(s)	94
BstHHI	GCGC	3 cut(s)	73, 93, 859
BstKTI	GATC	9 cut(s)	123, 132, 174, 755, 882, 1201, 1253, 1357, 1408
BstMAI	GTCTC	3 cut(s)	181, 257, 815
BstMBI	GATC	9 cut(s)	120, 129, 171, 752, 879, 1198, 1250, 1354, 1405
BstNI	CCWGG	4 cut(s)	113, 181, 1123, 1224
BstNSI	RCATGY	1 cut(s)	802
BstSCI	CCNGG	8 cut(s)	86, 93, 111, 123, 179, 525, 1121, 1222
BstSFI	CTRYAG	1 cut(s)	967
BstSLI	GKGCMC	2 cut(s)	106, 822
BstUI	CGCG	2 cut(s)	485, 857
BstV1I	GCAGC	8 cut(s)	329, 413, 487, 676, 706, 740, 1011, 1144
BstX2I	RGATCY	3 cut(s)	120, 129, 1405
BstYI	RGATCY	3 cut(s)	120, 129, 1405
Bsu36I	CCTNAGG	1 cut(s)	495
BsuI	GTATCC	1 cut(s)	830
BsuRI	GGCC	8 cut(s)	104, 116, 347, 374, 386, 1121, 1127, 1382
BtgI	CCRYGG	2 cut(s)	483, 510
BtgZI	GCGATG	1 cut(s)	543
BtsCI	GGATG	7 cut(s)	27, 655, 868, 946, 1066, 1191, 1288
BtsI	GCAGTG	1 cut(s)	792
BtsIMutI	CAGTG	5 cut(s)	615, 792, 849, 1056, 1167
BveI	ACCTGC	1 cut(s)	1303
Cac8I	GCNNGC	5 cut(s)	75, 141, 432, 579, 915
CaiI	CAGNNNCTG	1 cut(s)	719
CciI	TCATGA	1 cut(s)	1144
CfoI	GCGC	3 cut(s)	73, 93, 859
Cfr10I	RCCGGY	2 cut(s)	73, 139
Cfr13I	GGNCC	7 cut(s)	102, 103, 479, 490, 623, 1125, 1396
Cfr42I	CCGCGG	1 cut(s)	486
CseI	GACGC	4 cut(s)	211, 301, 519, 863
Csp6I	GTAC	3 cut(s)	871, 1150, 1161
CspCI	CAANNNNNGTGG	2 cut(s)	1251, 1286
CviAII	CATG	6 cut(s)	136, 157, 253, 799, 901, 1145
CviQI	GTAC	3 cut(s)	871, 1150, 1161
DdeI	CTNAG	8 cut(s)	146, 162, 495, 582, 645, 669, 1257, 1278
DinI	GGCGCC	1 cut(s)	92
DpnI	GATC	9 cut(s)	122, 131, 173, 754, 881, 1200, 1252, 1356, 1407
DpnII	GATC	9 cut(s)	120, 129, 171, 752, 879, 1198, 1250, 1354, 1405
DrdI	GACNNNNNNGTC	1 cut(s)	257
DriI	GACNNNNNGTC	1 cut(s)	604
DseDI	GACNNNNNNGTC	1 cut(s)	257
EaeI	YGGCCR	2 cut(s)	345, 1380
Eam1104I	CTCTTC	1 cut(s)	539
Eam1105I	GACNNNNNGTC	1 cut(s)	604
EarI	CTCTTC	1 cut(s)	539
EciI	GGCGGA	1 cut(s)	38
Eco130I	CCWWGG	3 cut(s)	210, 381, 1377
Eco24I	GRGCYC	1 cut(s)	106
Eco31I	GGTCTC	1 cut(s)	257
Eco47I	GGWCC	4 cut(s)	479, 490, 623, 1396
Eco57I	CTGAAG	1 cut(s)	1040
Eco81I	CCTNAGG	1 cut(s)	495
EcoO109I	RGGNCCY	3 cut(s)	102, 479, 1396
EcoRII	CCWGG	4 cut(s)	111, 179, 1121, 1222
EcoT14I	CCWWGG	3 cut(s)	210, 381, 1377
EcoT38I	GRGCYC	1 cut(s)	106
EgeI	GGCGCC	1 cut(s)	92
EheI	GGCGCC	1 cut(s)	92
ErhI	CCWWGG	3 cut(s)	210, 381, 1377
Esp3I	CGTCTC	1 cut(s)	181
FaeI	CATG	6 cut(s)	139, 160, 256, 802, 904, 1148
FalI	AAGNNNNNCTT	2 cut(s)	584, 616
FaqI	GGGAC	2 cut(s)	465, 476
FatI	CATG	6 cut(s)	135, 156, 252, 798, 900, 1144
FauI	CCCGC	3 cut(s)	439, 469, 490
FokI	GGATG	7 cut(s)	14, 662, 875, 933, 1073, 1198, 1295
FriOI	GRGCYC	1 cut(s)	106
FspBI	CTAG	1 cut(s)	1417
GlaI	GCGC	3 cut(s)	72, 92, 858
GsaI	CCCAGC	1 cut(s)	632
GsuI	CTGGAG	2 cut(s)	390, 396
HaeII	RGCGCY	1 cut(s)	94
HaeIII	GGCC	8 cut(s)	104, 116, 347, 374, 386, 1121, 1127, 1382
HapII	CCGG	8 cut(s)	74, 87, 94, 124, 140, 443, 455, 527
HgaI	GACGC	4 cut(s)	211, 301, 519, 863
HhaI	GCGC	3 cut(s)	73, 93, 859
Hin1I	GRCGYC	4 cut(s)	91, 258, 312, 530
Hin1II	CATG	6 cut(s)	139, 160, 256, 802, 904, 1148
Hin6I	GCGC	3 cut(s)	71, 91, 857
HinP1I	GCGC	3 cut(s)	71, 91, 857
HincII	GTYRAC	1 cut(s)	1075
HindII	GTYRAC	1 cut(s)	1075
HindIII	AAGCTT	2 cut(s)	575, 657
HinfI	GANTC	3 cut(s)	236, 549, 808
HpaII	CCGG	8 cut(s)	74, 87, 94, 124, 140, 443, 455, 527
HphI	GGTGA	1 cut(s)	838
Hpy166II	GTNNAC	4 cut(s)	598, 623, 958, 1075
Hpy188I	TCNGA	5 cut(s)	193, 241, 535, 830, 1250
Hpy188III	TCNNGA	6 cut(s)	262, 443, 467, 1058, 1092, 1145
Hpy8I	GTNNAC	4 cut(s)	598, 623, 958, 1075
HpyAV	CCTTC	6 cut(s)	20, 34, 277, 586, 883, 1096
HpyCH4III	ACNGT	6 cut(s)	278, 511, 679, 1034, 1171, 1267
HpyCH4IV	ACGT	1 cut(s)	258
HpyCH4V	TGCA	8 cut(s)	569, 778, 917, 929, 935, 1235, 1312, 1372
HpyF3I	CTNAG	8 cut(s)	146, 162, 495, 582, 645, 669, 1257, 1278
HpySE526I	ACGT	1 cut(s)	258
Hsp92I	GRCGYC	4 cut(s)	91, 258, 312, 530
Hsp92II	CATG	6 cut(s)	139, 160, 256, 802, 904, 1148
HspAI	GCGC	3 cut(s)	71, 91, 857
KasI	GGCGCC	1 cut(s)	90
KflI	GGGWCCC	1 cut(s)	479
Kpn2I	TCCGGA	1 cut(s)	442
KroI	GCCGGC	2 cut(s)	73, 139
KroNI	GCCGGC	2 cut(s)	75, 141
KspI	CCGCGG	1 cut(s)	486
Kzo9I	GATC	9 cut(s)	120, 129, 171, 752, 879, 1198, 1250, 1354, 1405
LmnI	GCTCC	6 cut(s)	155, 409, 415, 445, 445, 1349
Lsp1109I	GCAGC	8 cut(s)	329, 413, 487, 676, 706, 740, 1011, 1144
LweI	GCATC	4 cut(s)	566, 853, 1051, 1198
MaeI	CTAG	1 cut(s)	1417
MaeII	ACGT	1 cut(s)	258
MaeIII	GTNAC	4 cut(s)	53, 278, 895, 1321
MalI	GATC	9 cut(s)	122, 131, 173, 754, 881, 1200, 1252, 1356, 1407
MboI	GATC	9 cut(s)	120, 129, 171, 752, 879, 1198, 1250, 1354, 1405
MboII	GAAGA	4 cut(s)	124, 384, 556, 1312
MflI	RGATCY	3 cut(s)	120, 129, 1405
MhlI	GDGCHC	2 cut(s)	106, 822
MlsI	TGGCCA	1 cut(s)	1382
MluCI	AATT	5 cut(s)	388, 462, 741, 1236, 1341
MluNI	TGGCCA	1 cut(s)	1382
Mly113I	GGCGCC	1 cut(s)	91
MlyI	GAGTC	2 cut(s)	245, 817
MmeI	TCCRAC	2 cut(s)	685, 1228
Mox20I	TGGCCA	1 cut(s)	1382
MroI	TCCGGA	1 cut(s)	442
MroNI	GCCGGC	2 cut(s)	73, 139
MscI	TGGCCA	1 cut(s)	1382
MseI	TTAA	1 cut(s)	1005
Msp20I	TGGCCA	1 cut(s)	1382
MspA1I	CMGCKG	5 cut(s)	416, 485, 628, 719, 731
MspI	CCGG	8 cut(s)	74, 87, 94, 124, 140, 443, 455, 527
MspR9I	CCNGG	8 cut(s)	88, 95, 113, 125, 181, 527, 1123, 1224
MvaI	CCWGG	4 cut(s)	113, 181, 1123, 1224
MvnI	CGCG	2 cut(s)	485, 857
NaeI	GCCGGC	2 cut(s)	75, 141
NarI	GGCGCC	1 cut(s)	91
NciI	CCSGG	4 cut(s)	88, 95, 125, 527
NdeII	GATC	9 cut(s)	120, 129, 171, 752, 879, 1198, 1250, 1354, 1405
NgoMIV	GCCGGC	2 cut(s)	73, 139
NlaIII	CATG	6 cut(s)	139, 160, 256, 802, 904, 1148
NlaIV	GGNNCC	9 cut(s)	92, 104, 420, 447, 480, 481, 492, 762, 1398
NmeAIII	GCCGAG	1 cut(s)	394
NmuCI	GTSAC	1 cut(s)	895
NspI	RCATGY	1 cut(s)	802
PagI	TCATGA	1 cut(s)	1144
PcsI	WCGNNNNNNNCGW	3 cut(s)	231, 237, 366
PdiI	GCCGGC	2 cut(s)	75, 141
PfeI	GAWTC	1 cut(s)	549
PfoI	TCCNGGA	1 cut(s)	123
PleI	GAGTC	2 cut(s)	244, 816
PluTI	GGCGCC	1 cut(s)	94
PpsI	GAGTC	2 cut(s)	244, 816
PpuMI	RGGWCCY	2 cut(s)	479, 1396
Psp5II	RGGWCCY	2 cut(s)	479, 1396
Psp6I	CCWGG	4 cut(s)	111, 179, 1121, 1222
PspFI	CCCAGC	1 cut(s)	628
PspGI	CCWGG	4 cut(s)	111, 179, 1121, 1222
PspN4I	GGNNCC	9 cut(s)	92, 104, 420, 447, 480, 481, 492, 762, 1398
PspOMI	GGGCCC	1 cut(s)	102
PspPI	GGNCC	7 cut(s)	102, 103, 479, 490, 623, 1125, 1396
PspPPI	RGGWCCY	2 cut(s)	479, 1396
PstNI	CAGNNNCTG	1 cut(s)	719
PsuI	RGATCY	3 cut(s)	120, 129, 1405
RsaI	GTAC	3 cut(s)	872, 1151, 1162
RsaNI	GTAC	3 cut(s)	871, 1150, 1161
SacII	CCGCGG	1 cut(s)	486
SaqAI	TTAA	1 cut(s)	1005
Sau3AI	GATC	9 cut(s)	120, 129, 171, 752, 879, 1198, 1250, 1354, 1405
Sau96I	GGNCC	7 cut(s)	102, 103, 479, 490, 623, 1125, 1396
SchI	GAGTC	2 cut(s)	245, 817
ScrFI	CCNGG	8 cut(s)	88, 95, 113, 125, 181, 527, 1123, 1224
SduI	GDGCHC	2 cut(s)	106, 822
SfaNI	GCATC	4 cut(s)	566, 853, 1051, 1198
SfcI	CTRYAG	1 cut(s)	967
SfoI	GGCGCC	1 cut(s)	92
Sfr303I	CCGCGG	1 cut(s)	486
SgrBI	CCGCGG	1 cut(s)	486
SinI	GGWCC	4 cut(s)	479, 490, 623, 1396
SmlI	CTYRAG	1 cut(s)	803
SmoI	CTYRAG	1 cut(s)	803
Sse9I	AATT	5 cut(s)	388, 462, 741, 1236, 1341
SspDI	GGCGCC	1 cut(s)	90
SspMI	CTAG	1 cut(s)	1417
StyD4I	CCNGG	8 cut(s)	86, 93, 111, 123, 179, 525, 1121, 1222
StyI	CCWWGG	3 cut(s)	210, 381, 1377
TaaI	ACNGT	6 cut(s)	278, 511, 679, 1034, 1171, 1267
TaiI	ACGT	1 cut(s)	261
TaqI	TCGA	5 cut(s)	234, 261, 300, 887, 1029
TasI	AATT	5 cut(s)	388, 462, 741, 1236, 1341
TatI	WGTACW	2 cut(s)	870, 1149
TauI	GCSGC	4 cut(s)	312, 419, 506, 719
TfiI	GAWTC	1 cut(s)	549
Tru1I	TTAA	1 cut(s)	1005
Tru9I	TTAA	1 cut(s)	1005
TscAI	CASTG	5 cut(s)	615, 792, 856, 1056, 1174
TseFI	GTSAC	1 cut(s)	895
TseI	GCWGC	8 cut(s)	317, 401, 500, 689, 719, 728, 999, 1132
Tsp45I	GTSAC	1 cut(s)	895
TspDTI	ATGAA	4 cut(s)	16, 124, 384, 1353
TspRI	CASTG	5 cut(s)	615, 792, 856, 1056, 1174
VpaK11BI	GGWCC	4 cut(s)	479, 490, 623, 1396
XapI	RAATTY	1 cut(s)	462
XceI	RCATGY	1 cut(s)	802
XcmI	CCANNNNNNNNNTGG	1 cut(s)	856
XspI	CTAG	1 cut(s)	1417
ZraI	GACGTC	1 cut(s)	259

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.