Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0010794

Physical Location & Seq

Genomic Coordinates Reverse (-)

30413892 .. 30417139

Gene Structure 3248 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G015740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1407 bp

ATGGCTCACCTTCTCAACACCTCCTTCATCCCCGGCTCCCGAACCCTCGCCCGCATCCCGACTACTCCAGTCCTCATCTCCGGCCGCCGCAGCAGCAGGTCCCAAGTCCAGGCCAAGATCCGAGAAATCTTCATGCCGGCGCTCAGCTCCACCATGACCGAGGGCAAGATCGTGTCCTGGGTCAAGTCCGAGGGAGACAAGCTCTCCAAGGGCGAGAGCGTCGTCGTCGTCGAGTCCGATAAGGCTGACATGGACGTCGAGACTTTCTACGACGGGTACTTGGCCTCCATTATGGTCGAGGAAGGCGGCGTTGCCCCCGTCGGCTCCGCTATTGCGCTCTTGGCCGAGACCCAAGACGAGATCGCCGAGGCCAAGGCCAAAGCCACTTCTTCTTCTCCTTCTTCATCATCACCTCCTCAGGAGACCAAAATTCCGGAAAATGCGGCTCCCGCGGCGGCCCCTAAGGCGGCGGCGGCGGCGGCGGCGGCGGCAGCTCCGGTGGTGTCTTCTACACATCCGGCTTCAGAGGGAGGGAAGAGAATCGTGGCGTCACCTTACGCGAAGAAGCTGGCGAAGGAGTTGAAGGTTGAGTTGGGGGCAGTAGTGGGAACTGGGCCAATGGGGAGGATTGTGGCCAAGGATGTTGAGGCTTTTGCTGCAGCTCAGGCTCAGGCTCAGGCTCCGGCCGCCACATCGACGCCTGTGCCGGCCGCAGCGGCAGCATCAGGGATTGAGTTGGGGACTGTGGTGCCTTTTACTACCATGCAGGGCGCTGTGAGTAGGAACATGATTGAGAGTCTATCAGTGCCCACTTTCAGAGTAGGATACACTATCACCACTAATGCTCTGGATGCTCTCTACAAAAAGATCAAGTCCAAGGGAGTTACCATGACAGCATTGTTGGCAAAGGCAACAGCACTTGCATTGGTTCAACATCCTGTTGTCAACTCTGGCTGTAGAGATGGTAAGAGTTTTACTTATAACAGCAGCATTAACATAGCAGTTGCTGTGGCTATCGATGGTGGGTTGATAACACCAGTTCTTCAGGATGCAGACAAGGTTGACATATATACGTTGTCAAGAAAGTGGAAGGAGTTAGTTGATAAGGCCAGGGCCAAGCAGCTGCAACCTCATGAATACAATACAGGTACTTTCACTCTTTCAAATCTTGGGATGTTTGGCGTTGACCGGTTTGATGCCATTCTGCCACCTGGAACTGGAGCAATTATGGCAGTTGGAGCATCTCAGCCTACTGTTGTGGCTTCCAAGGATGGTCGAATTGGTATGAAGAACCAAATGCAGGTAAATGTTACAGCTGATCATCGTGTAATTTATGGTGCTGATCTTGCTTCGTTCTTGCAAACCTTGGCAAGAATTATTGAGGACCCCAAAGATCTTACCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005886 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016020 GO:0019904 GO:0022626 GO:0031967 GO:0031975 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0071944 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

468

Amino Acids

48.75

Weight (kDa)

8.85

Isoelectric Point (pI)

39.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	42 - 113	3.1e-15	Biotin-requiring enzyme
E3_binding	PF02817	181 - 216	3e-13	e3 binding domain
2-oxoacid_dh	PF00198	248 - 466	5.2e-63	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010794)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34430
fragaria_vesca	FvH4_2g12130
malus_domestica	MD05G1040900.v1.1 MD10G1046900.v1.1
prunus_persica	Prupe.8G056000_v2.0.a1
pyrus_communis	pycom05g03360 pycom10g03320
rosa_chinensis	RchiOBHm_Chr6g0272101 RchiOBHm_Chr6g0272111
rosa_laevigata	RLG00000013702
rosa_multiflora	Rmu_sc0013301.1_g000004 Rmu_sc0032599.1_g000001
rosa_roxburghii	Rroxscaffold_7G00196600
rosa_rugosa	Rorug06G0065600
rosa_samantha	Rh6AG182900 Rh6BG184200 Rh6CG184100 Rh6DG175700
rosa_wichuraiana	Rw0G009140 Rw6G015740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	981
AasI	GACNNNNNNGTC	1 cut(s)	254
AatII	GACGTC	1 cut(s)	258
Acc36I	ACCTGC	2 cut(s)	87, 1291
AccB1I	GGYRCC	1 cut(s)	748
AccII	CGCG	2 cut(s)	452, 560
AccIII	TCCGGA	1 cut(s)	433
AclWI	GGATC	1 cut(s)	112
AcoI	YGGCCR	5 cut(s)	82, 342, 633, 684, 708
AcsI	RAATTY	1 cut(s)	429
AcuI	CTGAAG	2 cut(s)	507, 1028
AcyI	GRCGYC	3 cut(s)	255, 548, 698
AfaI	GTAC	2 cut(s)	278, 1150
AfiI	CCNNNNNNNGG	4 cut(s)	109, 466, 1217, 1300
AgeI	ACCGGT	1 cut(s)	1188
AgsI	TTSAA	3 cut(s)	583, 932, 1164
AjnI	CCWGG	4 cut(s)	108, 176, 1109, 1210
AjuI	GAANNNNNNNTTGG	2 cut(s)	575, 607
AluBI	AGCT	7 cut(s)	147, 202, 494, 568, 662, 1123, 1316
AluI	AGCT	7 cut(s)	147, 202, 494, 568, 662, 1123, 1316
Alw26I	GTCTC	4 cut(s)	189, 254, 341, 416
AlwI	GGATC	1 cut(s)	112
AlwNI	CAGNNNCTG	1 cut(s)	1007
Aor13HI	TCCGGA	1 cut(s)	433
ApoI	RAATTY	1 cut(s)	429
AsiGI	ACCGGT	1 cut(s)	1188
AspLEI	GCGC	3 cut(s)	142, 337, 773
AspS9I	GGNCC	5 cut(s)	99, 457, 614, 1113, 1384
AsuC2I	CCSGG	1 cut(s)	33
AsuHPI	GGTGA	3 cut(s)	402, 543, 826
AvaII	GGWCC	2 cut(s)	99, 1384
AxyI	CCTNAGG	2 cut(s)	417, 462
BaeGI	GKGCMC	1 cut(s)	810
BalI	TGGCCA	1 cut(s)	635
BanI	GGYRCC	1 cut(s)	748
BbsI	GAAGAC	1 cut(s)	498
BccI	CCATC	3 cut(s)	956, 1013, 1265
BcgI	CGANNNNNNTGC	2 cut(s)	685, 719
BciT130I	CCWGG	4 cut(s)	110, 178, 1111, 1212
BciVI	GTATCC	1 cut(s)	818
BclI	TGATCA	1 cut(s)	1318
BcnI	CCSGG	1 cut(s)	33
BcoDI	GTCTC	4 cut(s)	189, 254, 341, 416
BfaI	CTAG	1 cut(s)	1405
BfmI	CTRYAG	2 cut(s)	657, 955
BfoI	RGCGCY	2 cut(s)	143, 774
BfuAI	ACCTGC	2 cut(s)	87, 1291
BfuI	GTATCC	1 cut(s)	818
BglI	GCCNNNNNGGC	2 cut(s)	464, 716
BglII	AGATCT	1 cut(s)	1393
BlpI	GCTNAGC	1 cut(s)	143
Bme1390I	CCNGG	5 cut(s)	33, 110, 178, 1111, 1212
Bme18I	GGWCC	2 cut(s)	99, 1384
BmgT120I	GGNCC	5 cut(s)	99, 457, 614, 1113, 1384
BmiI	GGNNCC	8 cut(s)	37, 101, 325, 447, 459, 681, 750, 1386
BmrFI	CCNGG	5 cut(s)	33, 110, 178, 1111, 1212
BmrI	ACTGGG	1 cut(s)	621
BmsI	GCATC	6 cut(s)	63, 731, 841, 1039, 1186, 1250
BmuI	ACTGGG	1 cut(s)	621
BpiI	GAAGAC	1 cut(s)	498
BplI	GAGNNNNNCTC	2 cut(s)	186, 218
BpmI	CTGGAG	2 cut(s)	51, 1239
Bpu10I	CCTNAGC	3 cut(s)	663, 669, 675
Bpu1102I	GCTNAGC	1 cut(s)	143
BpuMI	CCSGG	1 cut(s)	33
Bsa29I	ATCGAT	1 cut(s)	1017
BsaHI	GRCGYC	3 cut(s)	255, 548, 698
BsaI	GGTCTC	2 cut(s)	341, 416
BsaWI	WCCGGW	3 cut(s)	433, 496, 1188
BsaXI	ACNNNNNCTCC	4 cut(s)	188, 218, 269, 299
Bsc4I	CCNNNNNNNGG	4 cut(s)	109, 466, 1217, 1300
Bse118I	RCCGGY	3 cut(s)	136, 706, 1188
Bse1I	ACTGG	4 cut(s)	68, 616, 1037, 1222
Bse21I	CCTNAGG	2 cut(s)	417, 462
BseAI	TCCGGA	1 cut(s)	433
BseBI	CCWGG	4 cut(s)	110, 178, 1111, 1212
BseCI	ATCGAT	1 cut(s)	1017
BseGI	GGATG	9 cut(s)	27, 54, 514, 646, 856, 934, 1054, 1179, 1276
BseLI	CCNNNNNNNGG	4 cut(s)	109, 466, 1217, 1300
BseMII	CTCAG	6 cut(s)	157, 431, 677, 683, 689, 1259
BseNI	ACTGG	4 cut(s)	68, 616, 1037, 1222
BseRI	GAGGAG	1 cut(s)	405
BseSI	GKGCMC	1 cut(s)	810
BseX3I	CGGCCG	3 cut(s)	82, 684, 708
Bsh1236I	CGCG	2 cut(s)	452, 560
Bsh1285I	CGRYCG	3 cut(s)	85, 687, 711
BshNI	GGYRCC	1 cut(s)	748
BshTI	ACCGGT	1 cut(s)	1188
BshVI	ATCGAT	1 cut(s)	1017
BsiEI	CGRYCG	3 cut(s)	85, 687, 711
BsiSI	CCGG	9 cut(s)	33, 81, 137, 434, 497, 518, 683, 707, 1189
BslFI	GGGAC	2 cut(s)	85, 754
BslI	CCNNNNNNNGG	4 cut(s)	109, 466, 1217, 1300
BsmAI	GTCTC	4 cut(s)	189, 254, 341, 416
BsmFI	GGGAC	2 cut(s)	85, 754
Bso31I	GGTCTC	2 cut(s)	341, 416
Bsp1286I	GDGCHC	1 cut(s)	810
Bsp13I	TCCGGA	1 cut(s)	433
Bsp143I	GATC	7 cut(s)	117, 168, 360, 867, 1318, 1342, 1393
Bsp1720I	GCTNAGC	1 cut(s)	143
BspCNI	CTCAG	6 cut(s)	156, 430, 676, 682, 688, 1258
BspDI	ATCGAT	1 cut(s)	1017
BspEI	TCCGGA	1 cut(s)	433
BspFNI	CGCG	2 cut(s)	452, 560
BspHI	TCATGA	1 cut(s)	1132
BspLI	GGNNCC	8 cut(s)	37, 101, 325, 447, 459, 681, 750, 1386
BspMAI	CTGCAG	1 cut(s)	661
BspMI	ACCTGC	2 cut(s)	87, 1291
BspPI	GGATC	1 cut(s)	112
BspT107I	GGYRCC	1 cut(s)	748
BspTNI	GGTCTC	2 cut(s)	341, 416
BsrFI	RCCGGY	3 cut(s)	136, 706, 1188
BsrI	ACTGG	4 cut(s)	68, 616, 1037, 1222
BssAI	RCCGGY	3 cut(s)	136, 706, 1188
BssMI	GATC	7 cut(s)	117, 168, 360, 867, 1318, 1342, 1393
BssNI	GRCGYC	3 cut(s)	255, 548, 698
BssT1I	CCWWGG	6 cut(s)	207, 372, 636, 876, 1266, 1365
Bst2UI	CCWGG	4 cut(s)	110, 178, 1111, 1212
Bst4CI	ACNGT	2 cut(s)	745, 1255
Bst6I	CTCTTC	1 cut(s)	530
BstACI	GRCGYC	3 cut(s)	255, 548, 698
BstC8I	GCNNGC	4 cut(s)	52, 138, 570, 708
BstDEI	CTNAG	7 cut(s)	143, 417, 462, 663, 669, 675, 1245
BstDSI	CCRYGG	1 cut(s)	450
BstF5I	GGATG	9 cut(s)	27, 54, 514, 646, 856, 934, 1054, 1179, 1276
BstFNI	CGCG	2 cut(s)	452, 560
BstH2I	RGCGCY	2 cut(s)	143, 774
BstHHI	GCGC	3 cut(s)	142, 337, 773
BstKTI	GATC	7 cut(s)	120, 171, 363, 870, 1321, 1345, 1396
BstMAI	GTCTC	4 cut(s)	189, 254, 341, 416
BstMBI	GATC	7 cut(s)	117, 168, 360, 867, 1318, 1342, 1393
BstMCI	CGRYCG	3 cut(s)	85, 687, 711
BstNI	CCWGG	4 cut(s)	110, 178, 1111, 1212
BstSCI	CCNGG	5 cut(s)	31, 108, 176, 1109, 1210
BstSFI	CTRYAG	2 cut(s)	657, 955
BstSLI	GKGCMC	1 cut(s)	810
BstUI	CGCG	2 cut(s)	452, 560
BstV2I	GAAGAC	1 cut(s)	498
BstX2I	RGATCY	2 cut(s)	117, 1393
BstYI	RGATCY	2 cut(s)	117, 1393
BstZI	CGGCCG	3 cut(s)	82, 684, 708
Bsu15I	ATCGAT	1 cut(s)	1017
Bsu36I	CCTNAGG	2 cut(s)	417, 462
BsuI	GTATCC	1 cut(s)	818
BsuTUI	ATCGAT	1 cut(s)	1017
BtgI	CCRYGG	1 cut(s)	450
BtsCI	GGATG	9 cut(s)	27, 54, 514, 646, 856, 934, 1054, 1179, 1276
BtsIMutI	CAGTG	1 cut(s)	810
BveI	ACCTGC	2 cut(s)	87, 1291
Cac8I	GCNNGC	4 cut(s)	52, 138, 570, 708
CaiI	CAGNNNCTG	1 cut(s)	1007
CciI	TCATGA	1 cut(s)	1132
CfoI	GCGC	3 cut(s)	142, 337, 773
Cfr10I	RCCGGY	3 cut(s)	136, 706, 1188
Cfr13I	GGNCC	5 cut(s)	99, 457, 614, 1113, 1384
Cfr42I	CCGCGG	1 cut(s)	453
ClaI	ATCGAT	1 cut(s)	1017
CseI	GACGC	3 cut(s)	208, 537, 706
Csp6I	GTAC	2 cut(s)	277, 1149
CspAI	ACCGGT	1 cut(s)	1188
CviAII	CATG	7 cut(s)	133, 154, 250, 763, 787, 889, 1133
CviQI	GTAC	2 cut(s)	277, 1149
DdeI	CTNAG	7 cut(s)	143, 417, 462, 663, 669, 675, 1245
DpnI	GATC	7 cut(s)	119, 170, 362, 869, 1320, 1344, 1395
DpnII	GATC	7 cut(s)	117, 168, 360, 867, 1318, 1342, 1393
DrdI	GACNNNNNNGTC	1 cut(s)	254
DseDI	GACNNNNNNGTC	1 cut(s)	254
EaeI	YGGCCR	5 cut(s)	82, 342, 633, 684, 708
EagI	CGGCCG	3 cut(s)	82, 684, 708
Eam1104I	CTCTTC	1 cut(s)	530
EarI	CTCTTC	1 cut(s)	530
EclXI	CGGCCG	3 cut(s)	82, 684, 708
Eco130I	CCWWGG	6 cut(s)	207, 372, 636, 876, 1266, 1365
Eco31I	GGTCTC	2 cut(s)	341, 416
Eco47I	GGWCC	2 cut(s)	99, 1384
Eco52I	CGGCCG	3 cut(s)	82, 684, 708
Eco57I	CTGAAG	2 cut(s)	507, 1028
Eco81I	CCTNAGG	2 cut(s)	417, 462
EcoO109I	RGGNCCY	2 cut(s)	99, 1384
EcoRII	CCWGG	4 cut(s)	108, 176, 1109, 1210
EcoT14I	CCWWGG	6 cut(s)	207, 372, 636, 876, 1266, 1365
ErhI	CCWWGG	6 cut(s)	207, 372, 636, 876, 1266, 1365
FaeI	CATG	7 cut(s)	136, 157, 253, 766, 790, 892, 1136
FaqI	GGGAC	2 cut(s)	85, 754
FatI	CATG	7 cut(s)	132, 153, 249, 762, 786, 888, 1132
FauI	CCCGC	2 cut(s)	59, 457
FbaI	TGATCA	1 cut(s)	1318
FokI	GGATG	9 cut(s)	14, 41, 501, 653, 863, 921, 1061, 1186, 1283
FspBI	CTAG	1 cut(s)	1405
GlaI	GCGC	3 cut(s)	141, 336, 772
GsuI	CTGGAG	2 cut(s)	51, 1239
HaeII	RGCGCY	2 cut(s)	143, 774
HapII	CCGG	9 cut(s)	33, 81, 137, 434, 497, 518, 683, 707, 1189
HgaI	GACGC	3 cut(s)	208, 537, 706
HhaI	GCGC	3 cut(s)	142, 337, 773
Hin1I	GRCGYC	3 cut(s)	255, 548, 698
Hin1II	CATG	7 cut(s)	136, 157, 253, 766, 790, 892, 1136
Hin6I	GCGC	3 cut(s)	140, 335, 771
HinP1I	GCGC	3 cut(s)	140, 335, 771
HincII	GTYRAC	3 cut(s)	946, 1063, 1186
HindII	GTYRAC	3 cut(s)	946, 1063, 1186
HinfI	GANTC	3 cut(s)	233, 540, 796
HpaII	CCGG	9 cut(s)	33, 81, 137, 434, 497, 518, 683, 707, 1189
HphI	GGTGA	3 cut(s)	402, 543, 826
Hpy166II	GTNNAC	3 cut(s)	946, 1063, 1186
Hpy188I	TCNGA	5 cut(s)	122, 190, 238, 526, 818
Hpy188III	TCNNGA	9 cut(s)	39, 58, 259, 419, 434, 848, 1046, 1080, 1133
Hpy8I	GTNNAC	3 cut(s)	946, 1063, 1186
Hpy99I	CGWCG	8 cut(s)	224, 227, 230, 233, 260, 275, 323, 700
HpyAV	CCTTC	7 cut(s)	20, 34, 296, 408, 568, 577, 1084
HpyCH4III	ACNGT	2 cut(s)	745, 1255
HpyCH4IV	ACGT	2 cut(s)	255, 1073
HpyCH4V	TGCA	7 cut(s)	659, 766, 923, 1052, 1126, 1300, 1360
HpyF3I	CTNAG	7 cut(s)	143, 417, 462, 663, 669, 675, 1245
HpySE526I	ACGT	2 cut(s)	255, 1073
Hsp92I	GRCGYC	3 cut(s)	255, 548, 698
Hsp92II	CATG	7 cut(s)	136, 157, 253, 766, 790, 892, 1136
HspAI	GCGC	3 cut(s)	140, 335, 771
Kpn2I	TCCGGA	1 cut(s)	433
KroI	GCCGGC	2 cut(s)	136, 706
KroNI	GCCGGC	2 cut(s)	138, 708
Ksp22I	TGATCA	1 cut(s)	1318
KspI	CCGCGG	1 cut(s)	453
Kzo9I	GATC	7 cut(s)	117, 168, 360, 867, 1318, 1342, 1393
LmnI	GCTCC	8 cut(s)	41, 152, 329, 451, 499, 685, 1220, 1238
LweI	GCATC	6 cut(s)	63, 731, 841, 1039, 1186, 1250
MaeI	CTAG	1 cut(s)	1405
MaeII	ACGT	2 cut(s)	255, 1073
MaeIII	GTNAC	3 cut(s)	549, 883, 1309
MalI	GATC	7 cut(s)	119, 170, 362, 869, 1320, 1344, 1395
MboI	GATC	7 cut(s)	117, 168, 360, 867, 1318, 1342, 1393
MboII	GAAGA	9 cut(s)	121, 381, 384, 393, 498, 547, 574, 1034, 1300
MflI	RGATCY	2 cut(s)	117, 1393
MhlI	GDGCHC	1 cut(s)	810
MlsI	TGGCCA	1 cut(s)	635
MluCI	AATT	5 cut(s)	429, 1224, 1278, 1329, 1374
MluNI	TGGCCA	1 cut(s)	635
MlyI	GAGTC	2 cut(s)	242, 805
MmeI	TCCRAC	1 cut(s)	1216
Mox20I	TGGCCA	1 cut(s)	635
MroI	TCCGGA	1 cut(s)	433
MroNI	GCCGGC	2 cut(s)	136, 706
MscI	TGGCCA	1 cut(s)	635
MseI	TTAA	1 cut(s)	993
Msp20I	TGGCCA	1 cut(s)	635
MspA1I	CMGCKG	4 cut(s)	452, 716, 1123, 1316
MspI	CCGG	9 cut(s)	33, 81, 137, 434, 497, 518, 683, 707, 1189
MspR9I	CCNGG	5 cut(s)	33, 110, 178, 1111, 1212
MvaI	CCWGG	4 cut(s)	110, 178, 1111, 1212
MvnI	CGCG	2 cut(s)	452, 560
NaeI	GCCGGC	2 cut(s)	138, 708
NciI	CCSGG	1 cut(s)	33
NdeII	GATC	7 cut(s)	117, 168, 360, 867, 1318, 1342, 1393
NgoMIV	GCCGGC	2 cut(s)	136, 706
NlaIII	CATG	7 cut(s)	136, 157, 253, 766, 790, 892, 1136
NlaIV	GGNNCC	8 cut(s)	37, 101, 325, 447, 459, 681, 750, 1386
NmeAIII	GCCGAG	2 cut(s)	370, 391
NmuCI	GTSAC	1 cut(s)	549
PagI	TCATGA	1 cut(s)	1132
PcsI	WCGNNNNNNNCGW	3 cut(s)	228, 234, 363
PdiI	GCCGGC	2 cut(s)	138, 708
PfeI	GAWTC	1 cut(s)	540
PinAI	ACCGGT	1 cut(s)	1188
PleI	GAGTC	2 cut(s)	241, 804
PpsI	GAGTC	2 cut(s)	241, 804
PpuMI	RGGWCCY	2 cut(s)	99, 1384
PsiI	TTATAA	1 cut(s)	981
Psp5II	RGGWCCY	2 cut(s)	99, 1384
Psp6I	CCWGG	4 cut(s)	108, 176, 1109, 1210
PspGI	CCWGG	4 cut(s)	108, 176, 1109, 1210
PspN4I	GGNNCC	8 cut(s)	37, 101, 325, 447, 459, 681, 750, 1386
PspPI	GGNCC	5 cut(s)	99, 457, 614, 1113, 1384
PspPPI	RGGWCCY	2 cut(s)	99, 1384
PstI	CTGCAG	1 cut(s)	661
PstNI	CAGNNNCTG	1 cut(s)	1007
PsuI	RGATCY	2 cut(s)	117, 1393
PvuII	CAGCTG	2 cut(s)	1123, 1316
RsaI	GTAC	2 cut(s)	278, 1150
RsaNI	GTAC	2 cut(s)	277, 1149
SacII	CCGCGG	1 cut(s)	453
SaqAI	TTAA	1 cut(s)	993
Sau3AI	GATC	7 cut(s)	117, 168, 360, 867, 1318, 1342, 1393
Sau96I	GGNCC	5 cut(s)	99, 457, 614, 1113, 1384
SchI	GAGTC	2 cut(s)	242, 805
ScrFI	CCNGG	5 cut(s)	33, 110, 178, 1111, 1212
SduI	GDGCHC	1 cut(s)	810
SfaNI	GCATC	6 cut(s)	63, 731, 841, 1039, 1186, 1250
SfcI	CTRYAG	2 cut(s)	657, 955
Sfr303I	CCGCGG	1 cut(s)	453
SgrBI	CCGCGG	1 cut(s)	453
SinI	GGWCC	2 cut(s)	99, 1384
Sse9I	AATT	5 cut(s)	429, 1224, 1278, 1329, 1374
SspMI	CTAG	1 cut(s)	1405
StyD4I	CCNGG	5 cut(s)	31, 108, 176, 1109, 1210
StyI	CCWWGG	6 cut(s)	207, 372, 636, 876, 1266, 1365
TaaI	ACNGT	2 cut(s)	745, 1255
TaiI	ACGT	2 cut(s)	258, 1076
TaqI	TCGA	6 cut(s)	231, 258, 297, 695, 1017, 1276
TaqII	GACCGA	1 cut(s)	173
TasI	AATT	5 cut(s)	429, 1224, 1278, 1329, 1374
TfiI	GAWTC	1 cut(s)	540
Tru1I	TTAA	1 cut(s)	993
Tru9I	TTAA	1 cut(s)	993
TscAI	CASTG	1 cut(s)	810
TseFI	GTSAC	1 cut(s)	549
Tsp45I	GTSAC	1 cut(s)	549
TspDTI	ATGAA	5 cut(s)	16, 121, 393, 1149, 1301
TspRI	CASTG	1 cut(s)	810
VpaK11BI	GGWCC	2 cut(s)	99, 1384
XapI	RAATTY	1 cut(s)	429
XcmI	CCANNNNNNNNNTGG	1 cut(s)	844
XspI	CTAG	1 cut(s)	1405
ZraI	GACGTC	1 cut(s)	256

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.