AT1G34540

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008423
Physical Location & Seq
Forward (+)
12637054 .. 12638692
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G34540.1

Sequence Viewer

Length: 1497 bp
ATGGCGCTCTTAATATTCATCTTCCTCCTCTGTTTCCCTATCTCCATCTTCTTTATCTTCTTCACCAAGAAATCTTCTTCAGAATTTGGTTTCAAGTCGTATCCAATCGTCGGAAGCTTTCCTGGTCTTGTGAATAACCGTCACCGATTCCTCGACTGGACGGTGGAGACTCTTTCTCGATGTCCAACCCAAACGGCGATTTTCCGGCGACCAGGGAAGCAACAACTCATCATGACGGCGAATCCATCTAACGTCGAGTATATGCTCAAGACCAAATTCGAGAGCTTCCCTAAAGGCCAACAGTTTACTTCGGTTCTTGAGGATTTTCTCGGCCACGGGATTTTTAATTCCGATGGTGATATGTGGTGGAAACAGAGGAAGACGGCGAGCTACGAATTTAGTACTAAATCTCTCCGTGACTTTGTTATGAGCAACGTCACCGTCGAAATCAACACCAGGCTTGTTCCGGTGTTAGTAGAAGCTGCAACCACCGGAAAGTTGATAGATCTCCAAGACATATTGGAGCGATTCGCGTTCGATAACATCTGTAAATTAGCTTTCAATGTTGATTGCGCTTGTCTCGGCCACGACGGAGCGGTCGGTGTAAACTTCATGCGGGCCTTCGAAACCGCTGCGACGATTATATCGCAGCGGTTTCGGTCGGTGGCTTCGTGTGCATGGAGGATTAAAAAGAAACTTAACATTGGATCAGAGAGAGTTCTGAGAGAATCAATCGCGACCGTCCATAAATTCGCAGACGAGATCGTGCGTAACAGGATTGATCAAGGAAGATCAAGTGACCACAAGGAAGATTTGCTTTCTCGGTTTATCAGCAAGGAGGAGATGAATTCGCCGGAGATACTCCGTGACATTGTCATAAGTTTCATCCTCGCGGGACGAGACACGACTTCCTCTGCTTTGAGCTGGTTCTTTTGGTTACTCTCTATGCATCCAGAGGTGGAAGATAAGATTCTGCAAGAGCTAAACTCGATTCGCGCACGAACTGGGAAGCGAATCGGGGAAGTTTACGGGTTTGAACATCTTAAGATGATGAATTATCTTCACGCTGCTATAACTGAATCTTTAAGATTGTATCCACCTGTGCCAGTGGACATAAAGAGTTGTGCTGAGGACAATGTATTGCCTGATGGGACATTTGTAGGTAAAGGTTGGGCAATAACCTATAACATATTTGCAATGGGAAGGATGGAGAGTATTTGGGGGAAAGATTGTGATCGTTTTGATCCTGAGAGGTGGATTGATGAAACAAATGGTTGTTTTAGAGGTGAGGATCCTTCTAAGTTTCCTGCGTTTCATGCTGGACCAAGGATGTGTGTAGGGAAGGATATGGCTTATATTCAAATGAAATCGATAGTCGCTGCGGTTTTAGAGCGGTTTGTGGTTGAGGTTCCAGGGAAGGAGCGTCCTGAGATTCTATTGTCGATGACACTTAGAATCAAAGGAGGTTTGTTTGCGAGGGTACAAGAGAGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

498

Amino Acids

56.82

Weight (kDa)

8.57

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 481 1.9e-68 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 596
AccBSI CCGCTC 2 cut(s) 596, 1393
AccII CGCG 4 cut(s) 533, 737, 893, 996
AciI CCGC 7 cut(s) 596, 616, 630, 652, 893, 1382, 1393
AclWI GGATC 4 cut(s) 715, 1238, 1286, 1299
AcoI YGGCCR 2 cut(s) 331, 583
AcsI RAATTY 5 cut(s) 83, 275, 395, 749, 847
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 2 cut(s) 403, 1482
AfiI CCNNNNNNNGG 1 cut(s) 110
AflII CTTAAG 1 cut(s) 1043
AgsI TTSAA 4 cut(s) 94, 562, 1037, 1361
AjnI CCWGG 4 cut(s) 121, 211, 455, 1411
AluBI AGCT 7 cut(s) 117, 285, 390, 482, 557, 924, 982
AluI AGCT 7 cut(s) 117, 285, 390, 482, 557, 924, 982
Alw26I GTCTC 3 cut(s) 161, 584, 894
AlwI GGATC 4 cut(s) 715, 1238, 1286, 1299
AoxI GGCC 4 cut(s) 295, 331, 583, 618
ApeKI GCWGC 5 cut(s) 482, 632, 649, 1067, 1379
ApoI RAATTY 5 cut(s) 83, 275, 395, 749, 847
AspLEI GCGC 3 cut(s) 7, 575, 998
AspS9I GGNCC 2 cut(s) 618, 1322
AsuHPI GGTGA 5 cut(s) 55, 134, 368, 430, 1298
AsuII TTCGAA 1 cut(s) 624
AvaII GGWCC 1 cut(s) 1322
BamHI GGATCC 1 cut(s) 1291
BbsI GAAGAC 1 cut(s) 386
BbvCI CCTCAGC 1 cut(s) 1128
BbvI GCAGC 5 cut(s) 469, 619, 661, 1054, 1366
BccI CCATC 5 cut(s) 53, 253, 347, 1142, 1201
BceAI ACGGC 3 cut(s) 210, 252, 399
BcgI CGANNNNNNTGC 4 cut(s) 614, 638, 648, 672
BciT130I CCWGG 4 cut(s) 123, 213, 457, 1413
BciVI GTATCC 2 cut(s) 111, 1104
BclI TGATCA 1 cut(s) 781
BcoDI GTCTC 3 cut(s) 161, 584, 894
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 1043
BfuI GTATCC 2 cut(s) 111, 1104
BglII AGATCT 2 cut(s) 505, 1490
BisI GCNGC 5 cut(s) 483, 633, 650, 1068, 1380
BlsI GCNGC 5 cut(s) 484, 634, 651, 1069, 1381
BmcAI AGTACT 1 cut(s) 403
Bme1390I CCNGG 4 cut(s) 123, 213, 457, 1413
Bme18I GGWCC 1 cut(s) 1322
BmgT120I GGNCC 2 cut(s) 618, 1322
BmiI GGNNCC 2 cut(s) 1293, 1410
BmrFI CCNGG 4 cut(s) 123, 213, 457, 1413
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 1 cut(s) 958
BmuI ACTGGG 1 cut(s) 1014
BpiI GAAGAC 1 cut(s) 386
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
Bpu10I CCTNAGC 1 cut(s) 1128
Bpu14I TTCGAA 1 cut(s) 624
BpuEI CTTGAG 2 cut(s) 251, 338
Bsa29I ATCGAT 1 cut(s) 1370
BsaBI GATNNNNATC 1 cut(s) 1233
BsaJI CCNNGG 4 cut(s) 212, 334, 1325, 1412
BsaWI WCCGGW 2 cut(s) 466, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse1I ACTGG 3 cut(s) 161, 1009, 1106
Bse3DI GCAATG 1 cut(s) 1203
Bse8I GATNNNNATC 1 cut(s) 1233
BseBI CCWGG 4 cut(s) 123, 213, 457, 1413
BseCI ATCGAT 1 cut(s) 1370
BseDI CCNNGG 4 cut(s) 212, 334, 1325, 1412
BseGI GGATG 4 cut(s) 885, 949, 1212, 1335
BseJI GATNNNNATC 1 cut(s) 1233
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMI GCAATG 1 cut(s) 1203
BseMII CTCAG 4 cut(s) 713, 1119, 1239, 1419
BseNI ACTGG 3 cut(s) 161, 1009, 1106
BseRI GAGGAG 2 cut(s) 17, 854
BseXI GCAGC 5 cut(s) 469, 619, 661, 1054, 1366
Bsh1236I CGCG 4 cut(s) 533, 737, 893, 996
Bsh1285I CGRYCG 3 cut(s) 600, 662, 741
BshFI GGCC 4 cut(s) 297, 333, 585, 620
BshVI ATCGAT 1 cut(s) 1370
BsiEI CGRYCG 3 cut(s) 600, 662, 741
BsiSI CCGG 4 cut(s) 205, 467, 492, 854
BslFI GGGAC 2 cut(s) 909, 1165
BslI CCNNNNNNNGG 1 cut(s) 110
BsmAI GTCTC 3 cut(s) 161, 584, 894
BsmFI GGGAC 2 cut(s) 909, 1165
BsnI GGCC 4 cut(s) 297, 333, 585, 620
Bsp119I TTCGAA 1 cut(s) 624
Bsp143I GATC 9 cut(s) 505, 707, 762, 781, 791, 1234, 1243, 1291, 1490
Bsp68I TCGCGA 1 cut(s) 737
BspACI CCGC 7 cut(s) 596, 616, 630, 652, 893, 1382, 1393
BspANI GGCC 4 cut(s) 297, 333, 585, 620
BspCNI CTCAG 4 cut(s) 714, 1120, 1240, 1420
BspDI ATCGAT 1 cut(s) 1370
BspFNI CGCG 4 cut(s) 533, 737, 893, 996
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 1293, 1410
BspPI GGATC 4 cut(s) 715, 1238, 1286, 1299
BspT104I TTCGAA 1 cut(s) 624
BspTI CTTAAG 1 cut(s) 1043
BsrBI CCGCTC 2 cut(s) 596, 1393
BsrDI GCAATG 1 cut(s) 1203
BsrI ACTGG 3 cut(s) 161, 1009, 1106
BssECI CCNNGG 4 cut(s) 212, 334, 1325, 1412
BssMI GATC 9 cut(s) 505, 707, 762, 781, 791, 1234, 1243, 1291, 1490
BssT1I CCWWGG 1 cut(s) 1325
Bst2UI CCWGG 4 cut(s) 123, 213, 457, 1413
Bst4CI ACNGT 5 cut(s) 140, 163, 303, 442, 742
BstAFI CTTAAG 1 cut(s) 1043
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 2 cut(s) 388, 618
BstDEI CTNAG 6 cut(s) 722, 1128, 1248, 1299, 1428, 1451
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 4 cut(s) 885, 949, 1212, 1335
BstFNI CGCG 4 cut(s) 533, 737, 893, 996
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 575, 998
BstKTI GATC 9 cut(s) 508, 710, 765, 784, 794, 1237, 1246, 1294, 1493
BstMAI GTCTC 3 cut(s) 161, 584, 894
BstMBI GATC 9 cut(s) 505, 707, 762, 781, 791, 1234, 1243, 1291, 1490
BstMCI CGRYCG 3 cut(s) 600, 662, 741
BstMWI GCNNNNNNNGC 2 cut(s) 674, 1316
BstNI CCWGG 4 cut(s) 123, 213, 457, 1413
BstSCI CCNGG 4 cut(s) 121, 211, 455, 1411
BstUI CGCG 4 cut(s) 533, 737, 893, 996
BstV1I GCAGC 5 cut(s) 469, 619, 661, 1054, 1366
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 3 cut(s) 505, 1291, 1490
BstYI RGATCY 3 cut(s) 505, 1291, 1490
Bsu15I ATCGAT 1 cut(s) 1370
BsuI GTATCC 2 cut(s) 111, 1104
BsuRI GGCC 4 cut(s) 297, 333, 585, 620
BsuTUI ATCGAT 1 cut(s) 1370
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 4 cut(s) 885, 949, 1212, 1335
BtsIMutI CAGTG 1 cut(s) 1113
BtuMI TCGCGA 1 cut(s) 737
Cac8I GCNNGC 2 cut(s) 388, 618
CciI TCATGA 1 cut(s) 231
CfoI GCGC 3 cut(s) 7, 575, 998
Cfr13I GGNCC 2 cut(s) 618, 1322
ClaI ATCGAT 1 cut(s) 1370
CseI GACGC 1 cut(s) 1412
Csp6I GTAC 2 cut(s) 402, 1481
CviAII CATG 4 cut(s) 232, 613, 678, 1316
CviQI GTAC 2 cut(s) 402, 1481
DdeI CTNAG 6 cut(s) 722, 1128, 1248, 1299, 1428, 1451
DpnI GATC 9 cut(s) 507, 709, 764, 783, 793, 1236, 1245, 1293, 1492
DpnII GATC 9 cut(s) 505, 707, 762, 781, 791, 1234, 1243, 1291, 1490
DrdI GACNNNNNNGTC 1 cut(s) 596
DseDI GACNNNNNNGTC 1 cut(s) 596
EaeI YGGCCR 2 cut(s) 331, 583
Eco130I CCWWGG 1 cut(s) 1325
Eco47I GGWCC 1 cut(s) 1322
Eco57I CTGAAG 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 847
EcoRII CCWGG 4 cut(s) 121, 211, 455, 1411
EcoT14I CCWWGG 1 cut(s) 1325
EcoT22I ATGCAT 1 cut(s) 951
ErhI CCWWGG 1 cut(s) 1325
FaeI CATG 4 cut(s) 235, 616, 681, 1319
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 2 cut(s) 909, 1165
FatI CATG 4 cut(s) 231, 612, 677, 1315
FauI CCCGC 2 cut(s) 609, 886
FbaI TGATCA 1 cut(s) 781
Fnu4HI GCNGC 5 cut(s) 483, 633, 650, 1068, 1380
FokI GGATG 4 cut(s) 872, 936, 1219, 1342
Fsp4HI GCNGC 5 cut(s) 483, 633, 650, 1068, 1380
GlaI GCGC 3 cut(s) 6, 574, 997
GluI GCNGC 5 cut(s) 483, 633, 650, 1068, 1380
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 297, 333, 585, 620
HapII CCGG 4 cut(s) 205, 467, 492, 854
HgaI GACGC 1 cut(s) 1412
HhaI GCGC 3 cut(s) 7, 575, 998
Hin1II CATG 4 cut(s) 235, 616, 681, 1319
Hin6I GCGC 3 cut(s) 5, 573, 996
HinP1I GCGC 3 cut(s) 5, 573, 996
HindIII AAGCTT 1 cut(s) 115
HpaII CCGG 4 cut(s) 205, 467, 492, 854
HphI GGTGA 5 cut(s) 55, 134, 368, 430, 1298
Hpy166II GTNNAC 4 cut(s) 306, 607, 1027, 1111
Hpy188I TCNGA 5 cut(s) 82, 113, 352, 712, 723
Hpy8I GTNNAC 4 cut(s) 306, 607, 1027, 1111
Hpy99I CGWCG 5 cut(s) 113, 257, 446, 593, 640
HpyAV CCTTC 5 cut(s) 631, 1197, 1305, 1336, 1411
HpyCH4III ACNGT 5 cut(s) 140, 163, 303, 442, 742
HpyCH4IV ACGT 2 cut(s) 252, 435
HpyCH4V TGCA 5 cut(s) 485, 677, 949, 976, 1196
HpyF10VI GCNNNNNNNGC 2 cut(s) 674, 1316
HpyF3I CTNAG 6 cut(s) 722, 1128, 1248, 1299, 1428, 1451
HpySE526I ACGT 2 cut(s) 252, 435
Hsp92II CATG 4 cut(s) 235, 616, 681, 1319
HspAI GCGC 3 cut(s) 5, 573, 996
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 9 cut(s) 505, 707, 762, 781, 791, 1234, 1243, 1291, 1490
LmnI GCTCC 3 cut(s) 523, 593, 1420
Lsp1109I GCAGC 5 cut(s) 469, 619, 661, 1054, 1366
LweI GCATC 1 cut(s) 958
MaeII ACGT 2 cut(s) 252, 435
MaeIII GTNAC 7 cut(s) 140, 416, 436, 770, 797, 866, 936
MalI GATC 9 cut(s) 507, 709, 764, 783, 793, 1236, 1245, 1293, 1492
MbiI CCGCTC 2 cut(s) 596, 1393
MboI GATC 9 cut(s) 505, 707, 762, 781, 791, 1234, 1243, 1291, 1490
MflI RGATCY 3 cut(s) 505, 1291, 1490
MluCI AATT 8 cut(s) 83, 275, 346, 395, 551, 749, 847, 1054
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 2 cut(s) 91, 209
Mph1103I ATGCAT 1 cut(s) 951
MseI TTAA 6 cut(s) 11, 345, 687, 699, 1044, 1085
MspA1I CMGCKG 2 cut(s) 632, 652
MspCI CTTAAG 1 cut(s) 1043
MspI CCGG 4 cut(s) 205, 467, 492, 854
MspR9I CCNGG 4 cut(s) 123, 213, 457, 1413
MvaI CCWGG 4 cut(s) 123, 213, 457, 1413
MvnI CGCG 4 cut(s) 533, 737, 893, 996
MwoI GCNNNNNNNGC 2 cut(s) 674, 1316
NdeII GATC 9 cut(s) 505, 707, 762, 781, 791, 1234, 1243, 1291, 1490
NlaIII CATG 4 cut(s) 235, 616, 681, 1319
NlaIV GGNNCC 2 cut(s) 1293, 1410
NmeAIII GCCGAG 2 cut(s) 309, 561
NmuCI GTSAC 5 cut(s) 140, 416, 436, 797, 866
NruI TCGCGA 1 cut(s) 737
NsiI ATGCAT 1 cut(s) 951
NspV TTCGAA 1 cut(s) 624
PagI TCATGA 1 cut(s) 231
PcsI WCGNNNNNNNCGW 2 cut(s) 441, 668
PflFI GACNNNGTC 1 cut(s) 872
PkrI GCNGC 5 cut(s) 484, 634, 651, 1069, 1381
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp6I CCWGG 4 cut(s) 121, 211, 455, 1411
PspGI CCWGG 4 cut(s) 121, 211, 455, 1411
PspN4I GGNNCC 2 cut(s) 1293, 1410
PspPI GGNCC 2 cut(s) 618, 1322
PsuI RGATCY 3 cut(s) 505, 1291, 1490
PsyI GACNNNGTC 1 cut(s) 872
RruI TCGCGA 1 cut(s) 737
RsaI GTAC 2 cut(s) 403, 1482
RsaNI GTAC 2 cut(s) 402, 1481
SaqAI TTAA 6 cut(s) 11, 345, 687, 699, 1044, 1085
SatI GCNGC 5 cut(s) 483, 633, 650, 1068, 1380
Sau3AI GATC 9 cut(s) 505, 707, 762, 781, 791, 1234, 1243, 1291, 1490
Sau96I GGNCC 2 cut(s) 618, 1322
ScaI AGTACT 1 cut(s) 403
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 4 cut(s) 123, 213, 457, 1413
SfaNI GCATC 1 cut(s) 958
SfuI TTCGAA 1 cut(s) 624
SinI GGWCC 1 cut(s) 1322
SmlI CTYRAG 3 cut(s) 266, 317, 1043
SmoI CTYRAG 3 cut(s) 266, 317, 1043
Sse9I AATT 8 cut(s) 83, 275, 346, 395, 551, 749, 847, 1054
SsiI CCGC 7 cut(s) 596, 616, 630, 652, 893, 1382, 1393
SspI AATATT 1 cut(s) 15
StyD4I CCNGG 4 cut(s) 121, 211, 455, 1411
StyI CCWWGG 1 cut(s) 1325
TaaI ACNGT 5 cut(s) 140, 163, 303, 442, 742
TaiI ACGT 2 cut(s) 255, 438
TaqII GACCGA 1 cut(s) 648
TasI AATT 8 cut(s) 83, 275, 346, 395, 551, 749, 847, 1054
TatI WGTACW 1 cut(s) 401
Tru1I TTAA 6 cut(s) 11, 345, 687, 699, 1044, 1085
Tru9I TTAA 6 cut(s) 11, 345, 687, 699, 1044, 1085
TscAI CASTG 1 cut(s) 1113
TseFI GTSAC 5 cut(s) 140, 416, 436, 797, 866
TseI GCWGC 5 cut(s) 482, 632, 649, 1067, 1379
Tsp45I GTSAC 5 cut(s) 140, 416, 436, 797, 866
TspDTI ATGAA 8 cut(s) 7, 601, 860, 874, 1067, 1278, 1304, 1379
TspGWI ACGGA 3 cut(s) 404, 606, 854
TspRI CASTG 1 cut(s) 1113
Tth111I GACNNNGTC 1 cut(s) 872
Vha464I CTTAAG 1 cut(s) 1043
VpaK11BI GGWCC 1 cut(s) 1322
XapI RAATTY 5 cut(s) 83, 275, 395, 749, 847
ZrmI AGTACT 1 cut(s) 403
Zsp2I ATGCAT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.