pycom06g18960

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008423
Physical Location & Seq
Reverse (-)
22523471 .. 22525024
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g18960.1

Sequence Viewer

Length: 1554 bp
ATGGAACTCTTCTCTCTACAGTCCTTCCTCCTCTTCCTCTTGCTCTCCATCTACCTTTACTTCCACTTCTTCGCTTCGCCGAAGAAATATAAACACACCAGCTTCAAATACTACCCGATTATTGGAACCCTACCGGAATTCCTAAAAAATCGCCACCGGTTTCTCGAATGGACAACCGAAATCCTCATCAACTGCCCCACCAGCACCGCCATCTTCAGCCGCCCAGTCAAAATCCGTGGCATCATCACCGCCAACCCTGCCAACGTCGAGCACATGCTGAAAACCAACTTCAAAAACTACCCGAAAGGCGAAAGGGTCATATCCCTCCTTCAAGGCTTTCTCGGCGGCGGAATCTTCAACTCCGACGGCGAGCTCTGGATGGTCCAGAGGAAGACCGCCAGCTACGCCTTCAACACGAAATCGCTCCGGAACTTCGTGATGGAAAACGTTAGGTTTGAGATTGAGTTGAGGCTAATCCCGTGTTTGGACACAGCCGCTGAAACGGGGCGTGGTTTGGACTTGCAAGACGTTTTGGAGCGCTTTGCTTTCGACAACGTTTGCAAGTTGGCTTTTAACGTCGACCCGAATTGTCTTGCAATCGGCGGAAAATCTGAGTCCGCCGATTTCGATTCCAATTCAGATCAGTTCATGCAGGCATTTGAAGAAGCAGCCACGCTTAGTTCCGGGAGATTTTTGTATGCGTTTAGGTTTATACAAAGGATTAAGAAGTTTTTGAACGTTGGATCAGAGCGGAGGCTAAAAGATTCAATATCGACCGTCCATAATTTCGCGGATAATATAATAAAGTCAAGAATTAGCGACGATCGACGGTCGGAGGATCGTCACGATTTGTTGTCCCTGTTTATCGGTATTCCTGAAAACAACAGCTCGCCCGAGTTTCTCCGCGATATCGTGATAAGCATCATTCTGGCCGGCCGAGACACGACATCATCGGCTCTGACATGGTTCTTCTGGCTCCTGTCGTCCAGACCGCACGTGGAGCGCGCCATTTTAAAGGAGCTCGAAACGATTCGGGTCCGAAACGGGAAACGTATCGGTGATATTTACGATTTGGACGAGCTCCGAGACATGCACTATTTGCACGCGGCGATTTCTGAGGCGATGCGGCTGTACCCTCCAGTACCGGTCGACACGAAGGCTTGTCTAAACGACGACGTCCTGCCGGACGGTACGTTCGTGGGGAAGGGCTGGTTCGTGACGTACCATGCCTACGCAATGGGGAGGATGGAGAAGATATGGGGGAAGAACTGCCGTGAGTATCTGCCTGAGCGGTGGCTGACGGAGGACGGCACGTGTCGGCAGGAGAATGTTTTTCGGTATCCGATATTTCATGGCGGACCAAGGATGTGTCTCGGGAAGGATTTGGCGTATATTCAGATGAAGTCGATTGCAGCGTCCGTGATGGAGCGGTTTGAGATCGACGTTCAGGATAGGGACACGTGTCCTGAGCACCTATTGTCATTGACTCTCAGGATGAAAGGTGGACTGCCAGTGAGTGTGAGGAGGAGGAGATGTGTTGCTGATGATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

518

Amino Acids

59.65

Weight (kDa)

8.55

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 82 - 490 7.9e-59 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1179
AccBSI CCGCTC 3 cut(s) 751, 1291, 1429
AccI GTMKAC 2 cut(s) 579, 1149
AccII CGCG 4 cut(s) 791, 906, 1005, 1106
AccIII TCCGGA 1 cut(s) 426
AclI AACGTT 3 cut(s) 447, 555, 738
AclWI GGATC 2 cut(s) 751, 846
AcoI YGGCCR 2 cut(s) 930, 934
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 199
AcvI CACGTG 3 cut(s) 997, 1314, 1461
AcyI GRCGYC 1 cut(s) 1176
AfaI GTAC 4 cut(s) 1133, 1143, 1192, 1223
AfeI AGCGCT 1 cut(s) 539
AfiI CCNNNNNNNGG 2 cut(s) 122, 484
AflIII ACRYGT 3 cut(s) 1313, 1458, 1460
AgeI ACCGGT 2 cut(s) 156, 1144
AgsI TTSAA 8 cut(s) 106, 292, 332, 358, 412, 662, 736, 768
AloI GAACNNNNNNTCC 2 cut(s) 1178, 1210
AluBI AGCT 6 cut(s) 102, 373, 402, 888, 1021, 1081
AluI AGCT 6 cut(s) 102, 373, 402, 888, 1021, 1081
Alw21I GWGCWC 5 cut(s) 273, 375, 1023, 1083, 1473
Alw26I GTCTC 3 cut(s) 933, 1080, 1376
AlwI GGATC 2 cut(s) 751, 846
AlwNI CAGNNNCTG 1 cut(s) 497
Ama87I CYCGRG 2 cut(s) 893, 1373
Aor13HI TCCGGA 1 cut(s) 426
Aor51HI AGCGCT 1 cut(s) 539
AoxI GGCC 2 cut(s) 930, 934
ApeKI GCWGC 2 cut(s) 668, 1412
ApoI RAATTY 1 cut(s) 137
ArsI GACNNNNNNTTYG 2 cut(s) 1178, 1210
AsiGI ACCGGT 2 cut(s) 156, 1144
Asp700I GAANNNNTTC 1 cut(s) 1029
AspLEI GCGC 3 cut(s) 540, 1005, 1007
AspS9I GGNCC 3 cut(s) 382, 1036, 1358
AsuC2I CCSGG 1 cut(s) 685
AsuHPI GGTGA 2 cut(s) 238, 1070
AvaI CYCGRG 2 cut(s) 893, 1373
AvaII GGWCC 3 cut(s) 382, 1036, 1358
BaeI ACNNNNGTAYC 2 cut(s) 1182, 1215
BanII GRGCYC 3 cut(s) 375, 1023, 1083
BbrPI CACGTG 3 cut(s) 997, 1314, 1461
BbsI GAAGAC 1 cut(s) 398
Bbv12I GWGCWC 5 cut(s) 273, 375, 1023, 1083, 1473
BbvI GCAGC 2 cut(s) 680, 1424
BccI CCATC 6 cut(s) 56, 218, 373, 433, 1240, 1417
BceAI ACGGC 3 cut(s) 382, 1257, 1324
BciVI GTATCC 1 cut(s) 1350
BcnI CCSGG 1 cut(s) 685
BcoDI GTCTC 3 cut(s) 933, 1080, 1376
BfmI CTRYAG 1 cut(s) 17
BfoI RGCGCY 1 cut(s) 541
BfuI GTATCC 1 cut(s) 1350
BisI GCNGC 7 cut(s) 220, 346, 495, 669, 1107, 1127, 1413
BlsI GCNGC 7 cut(s) 221, 347, 496, 670, 1108, 1128, 1414
Bme1390I CCNGG 1 cut(s) 685
Bme18I GGWCC 3 cut(s) 382, 1036, 1358
BmeT110I CYCGRG 2 cut(s) 893, 1373
BmgT120I GGNCC 3 cut(s) 382, 1036, 1358
BmiI GGNNCC 3 cut(s) 127, 977, 1037
BmrFI CCNGG 1 cut(s) 685
BmrI ACTGGG 1 cut(s) 218
BmsI GCATC 3 cut(s) 249, 930, 1113
BmuI ACTGGG 1 cut(s) 218
BoxI GACNNNNGTC 1 cut(s) 1461
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 1122
Bpu10I CCTNAGC 2 cut(s) 1287, 1467
BpuMI CCSGG 1 cut(s) 685
BsaAI YACGTR 3 cut(s) 997, 1314, 1461
BsaBI GATNNNNATC 1 cut(s) 920
BsaHI GRCGYC 1 cut(s) 1176
BsaJI CCNNGG 2 cut(s) 235, 1361
BsaWI WCCGGW 4 cut(s) 133, 156, 426, 1144
BsaXI ACNNNNNCTCC 2 cut(s) 1519, 1549
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 484
Bse118I RCCGGY 3 cut(s) 156, 932, 1144
Bse1I ACTGG 3 cut(s) 224, 1139, 1511
Bse3DI GCAATG 1 cut(s) 1242
Bse8I GATNNNNATC 1 cut(s) 920
BseAI TCCGGA 1 cut(s) 426
BseDI CCNNGG 2 cut(s) 235, 1361
BseGI GGATG 4 cut(s) 384, 1251, 1371, 1500
BseJI GATNNNNATC 1 cut(s) 920
BseLI CCNNNNNNNGG 2 cut(s) 122, 484
BseMI GCAATG 1 cut(s) 1242
BseMII CTCAG 5 cut(s) 603, 1107, 1278, 1458, 1504
BseNI ACTGG 3 cut(s) 224, 1139, 1511
BsePI GCGCGC 1 cut(s) 1003
BseRI GAGGAG 4 cut(s) 20, 1537, 1540, 1543
BseX3I CGGCCG 1 cut(s) 934
BseXI GCAGC 2 cut(s) 680, 1424
Bsh1236I CGCG 4 cut(s) 791, 906, 1005, 1106
Bsh1285I CGRYCG 5 cut(s) 777, 826, 833, 937, 1149
BshFI GGCC 2 cut(s) 932, 936
BshTI ACCGGT 2 cut(s) 156, 1144
BsiEI CGRYCG 5 cut(s) 777, 826, 833, 937, 1149
BsiHKAI GWGCWC 5 cut(s) 273, 375, 1023, 1083, 1473
BsiHKCI CYCGRG 2 cut(s) 893, 1373
BsiSI CCGG 7 cut(s) 134, 157, 427, 684, 933, 1145, 1184
BslFI GGGAC 2 cut(s) 841, 1469
BslI CCNNNNNNNGG 2 cut(s) 122, 484
BsmAI GTCTC 3 cut(s) 933, 1080, 1376
BsmFI GGGAC 2 cut(s) 841, 1469
BsnI GGCC 2 cut(s) 932, 936
BsoBI CYCGRG 2 cut(s) 893, 1373
Bsp1286I GDGCHC 5 cut(s) 273, 375, 1023, 1083, 1473
Bsp13I TCCGGA 1 cut(s) 426
Bsp143I GATC 6 cut(s) 640, 743, 823, 838, 1437, 1546
BspANI GGCC 2 cut(s) 932, 936
BspCNI CTCAG 5 cut(s) 604, 1108, 1279, 1459, 1503
BspEI TCCGGA 1 cut(s) 426
BspFNI CGCG 4 cut(s) 791, 906, 1005, 1106
BspLI GGNNCC 3 cut(s) 127, 977, 1037
BspPI GGATC 2 cut(s) 751, 846
BsrBI CCGCTC 3 cut(s) 751, 1291, 1429
BsrDI GCAATG 1 cut(s) 1242
BsrFI RCCGGY 3 cut(s) 156, 932, 1144
BsrI ACTGG 3 cut(s) 224, 1139, 1511
BssAI RCCGGY 3 cut(s) 156, 932, 1144
BssECI CCNNGG 2 cut(s) 235, 1361
BssHII GCGCGC 1 cut(s) 1003
BssMI GATC 6 cut(s) 640, 743, 823, 838, 1437, 1546
BssNI GRCGYC 1 cut(s) 1176
BssT1I CCWWGG 1 cut(s) 1361
Bst4CI ACNGT 4 cut(s) 21, 778, 831, 1190
Bst6I CTCTTC 2 cut(s) 14, 38
BstACI GRCGYC 1 cut(s) 1176
BstAPI GCANNNNNTGC 1 cut(s) 1099
BstBAI YACGTR 3 cut(s) 997, 1314, 1461
BstC8I GCNNGC 7 cut(s) 371, 400, 654, 890, 934, 1005, 1104
BstDEI CTNAG 6 cut(s) 612, 677, 1116, 1287, 1467, 1490
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 4 cut(s) 384, 1251, 1371, 1500
BstFNI CGCG 4 cut(s) 791, 906, 1005, 1106
BstH2I RGCGCY 1 cut(s) 541
BstHHI GCGC 3 cut(s) 540, 1005, 1007
BstKTI GATC 6 cut(s) 643, 746, 826, 841, 1440, 1549
BstMAI GTCTC 3 cut(s) 933, 1080, 1376
BstMBI GATC 6 cut(s) 640, 743, 823, 838, 1437, 1546
BstMCI CGRYCG 5 cut(s) 777, 826, 833, 937, 1149
BstMWI GCNNNNNNNGC 6 cut(s) 201, 257, 342, 404, 1000, 1099
BstNSI RCATGY 2 cut(s) 277, 1093
BstPAI GACNNNNGTC 1 cut(s) 1461
BstSCI CCNGG 1 cut(s) 683
BstSFI CTRYAG 1 cut(s) 17
BstUI CGCG 4 cut(s) 791, 906, 1005, 1106
BstV1I GCAGC 2 cut(s) 680, 1424
BstV2I GAAGAC 1 cut(s) 398
BstZI CGGCCG 1 cut(s) 934
BsuI GTATCC 1 cut(s) 1350
BsuRI GGCC 2 cut(s) 932, 936
BtgI CCRYGG 1 cut(s) 235
BtgZI GCGATG 1 cut(s) 1136
BtsCI GGATG 4 cut(s) 384, 1251, 1371, 1500
BtsIMutI CAGTG 1 cut(s) 1518
Cac8I GCNNGC 7 cut(s) 371, 400, 654, 890, 934, 1005, 1104
CaiI CAGNNNCTG 1 cut(s) 497
CfoI GCGC 3 cut(s) 540, 1005, 1007
Cfr10I RCCGGY 3 cut(s) 156, 932, 1144
Cfr13I GGNCC 3 cut(s) 382, 1036, 1358
CseI GACGC 1 cut(s) 1404
Csp6I GTAC 4 cut(s) 1132, 1142, 1191, 1222
CspAI ACCGGT 2 cut(s) 156, 1144
CspCI CAANNNNNGTGG 2 cut(s) 217, 252
CviAII CATG 6 cut(s) 274, 649, 963, 1090, 1226, 1352
CviQI GTAC 4 cut(s) 1132, 1142, 1191, 1222
DdeI CTNAG 6 cut(s) 612, 677, 1116, 1287, 1467, 1490
DpnI GATC 6 cut(s) 642, 745, 825, 840, 1439, 1548
DpnII GATC 6 cut(s) 640, 743, 823, 838, 1437, 1546
DraI TTTAAA 1 cut(s) 1014
EaeI YGGCCR 2 cut(s) 930, 934
EagI CGGCCG 1 cut(s) 934
Eam1104I CTCTTC 2 cut(s) 14, 38
EarI CTCTTC 2 cut(s) 14, 38
EciI GGCGGA 4 cut(s) 363, 607, 618, 1371
Ecl136II GAGCTC 3 cut(s) 373, 1021, 1081
EclXI CGGCCG 1 cut(s) 934
Eco130I CCWWGG 1 cut(s) 1361
Eco24I GRGCYC 3 cut(s) 375, 1023, 1083
Eco32I GATATC 1 cut(s) 910
Eco47I GGWCC 3 cut(s) 382, 1036, 1358
Eco47III AGCGCT 1 cut(s) 539
Eco52I CGGCCG 1 cut(s) 934
Eco53kI GAGCTC 3 cut(s) 373, 1021, 1081
Eco57I CTGAAG 1 cut(s) 199
Eco72I CACGTG 3 cut(s) 997, 1314, 1461
Eco88I CYCGRG 2 cut(s) 893, 1373
EcoICRI GAGCTC 3 cut(s) 373, 1021, 1081
EcoRI GAATTC 1 cut(s) 137
EcoRV GATATC 1 cut(s) 910
EcoT14I CCWWGG 1 cut(s) 1361
EcoT38I GRGCYC 3 cut(s) 375, 1023, 1083
ErhI CCWWGG 1 cut(s) 1361
FaeI CATG 6 cut(s) 277, 652, 966, 1093, 1229, 1355
FaqI GGGAC 2 cut(s) 841, 1469
FatI CATG 6 cut(s) 273, 648, 962, 1089, 1225, 1351
FblI GTMKAC 2 cut(s) 579, 1149
Fnu4HI GCNGC 7 cut(s) 220, 346, 495, 669, 1107, 1127, 1413
FokI GGATG 4 cut(s) 391, 1258, 1378, 1507
FriOI GRGCYC 3 cut(s) 375, 1023, 1083
FseI GGCCGGCC 1 cut(s) 936
Fsp4HI GCNGC 7 cut(s) 220, 346, 495, 669, 1107, 1127, 1413
GlaI GCGC 3 cut(s) 539, 1004, 1006
GluI GCNGC 7 cut(s) 220, 346, 495, 669, 1107, 1127, 1413
GsuI CTGGAG 1 cut(s) 1122
HaeII RGCGCY 1 cut(s) 541
HaeIII GGCC 2 cut(s) 932, 936
HapII CCGG 7 cut(s) 134, 157, 427, 684, 933, 1145, 1184
HgaI GACGC 1 cut(s) 1404
HhaI GCGC 3 cut(s) 540, 1005, 1007
Hin1I GRCGYC 1 cut(s) 1176
Hin1II CATG 6 cut(s) 277, 652, 966, 1093, 1229, 1355
Hin6I GCGC 3 cut(s) 538, 1003, 1005
HinP1I GCGC 3 cut(s) 538, 1003, 1005
HincII GTYRAC 2 cut(s) 580, 1150
HindII GTYRAC 2 cut(s) 580, 1150
HinfI GANTC 6 cut(s) 351, 614, 629, 764, 1030, 1486
HpaII CCGG 7 cut(s) 134, 157, 427, 684, 933, 1145, 1184
HphI GGTGA 2 cut(s) 238, 1070
Hpy166II GTNNAC 3 cut(s) 580, 1150, 1505
Hpy8I GTNNAC 3 cut(s) 580, 1150, 1505
Hpy99I CGWCG 8 cut(s) 269, 368, 581, 824, 831, 1175, 1178, 1445
HpyAV CCTTC 6 cut(s) 34, 338, 418, 1150, 1198, 1372
HpyCH4III ACNGT 4 cut(s) 21, 778, 831, 1190
HpyCH4V TGCA 7 cut(s) 523, 561, 596, 652, 1093, 1102, 1412
HpyF10VI GCNNNNNNNGC 6 cut(s) 201, 257, 342, 404, 1000, 1099
HpyF3I CTNAG 6 cut(s) 612, 677, 1116, 1287, 1467, 1490
Hsp92I GRCGYC 1 cut(s) 1176
Hsp92II CATG 6 cut(s) 277, 652, 966, 1093, 1229, 1355
HspAI GCGC 3 cut(s) 538, 1003, 1005
Kpn2I TCCGGA 1 cut(s) 426
KroI GCCGGC 1 cut(s) 932
KroNI GCCGGC 1 cut(s) 934
Kzo9I GATC 6 cut(s) 640, 743, 823, 838, 1437, 1546
LmnI GCTCC 7 cut(s) 429, 535, 981, 1000, 1018, 1086, 1426
Lsp1109I GCAGC 2 cut(s) 680, 1424
LweI GCATC 3 cut(s) 249, 930, 1113
MaeIII GTNAC 2 cut(s) 842, 1216
MalI GATC 6 cut(s) 642, 745, 825, 840, 1439, 1548
MbiI CCGCTC 3 cut(s) 751, 1291, 1429
MboI GATC 6 cut(s) 640, 743, 823, 838, 1437, 1546
MhlI GDGCHC 5 cut(s) 273, 375, 1023, 1083, 1473
MluCI AATT 5 cut(s) 137, 586, 634, 784, 813
MlyI GAGTC 2 cut(s) 623, 1480
MmeI TCCRAC 3 cut(s) 387, 721, 813
MroI TCCGGA 1 cut(s) 426
MroNI GCCGGC 1 cut(s) 932
MroXI GAANNNNTTC 1 cut(s) 1029
MseI TTAA 3 cut(s) 573, 723, 1013
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 7 cut(s) 134, 157, 427, 684, 933, 1145, 1184
MspR9I CCNGG 1 cut(s) 685
MvnI CGCG 4 cut(s) 791, 906, 1005, 1106
MwoI GCNNNNNNNGC 6 cut(s) 201, 257, 342, 404, 1000, 1099
NaeI GCCGGC 1 cut(s) 934
NciI CCSGG 1 cut(s) 685
NdeII GATC 6 cut(s) 640, 743, 823, 838, 1437, 1546
NgoMIV GCCGGC 1 cut(s) 932
NlaIII CATG 6 cut(s) 277, 652, 966, 1093, 1229, 1355
NlaIV GGNNCC 3 cut(s) 127, 977, 1037
NmeAIII GCCGAG 2 cut(s) 321, 962
NmuCI GTSAC 2 cut(s) 842, 1216
NspI RCATGY 2 cut(s) 277, 1093
PauI GCGCGC 1 cut(s) 1003
PcsI WCGNNNNNNNCGW 3 cut(s) 1074, 1194, 1412
PdiI GCCGGC 1 cut(s) 934
PdmI GAANNNNTTC 1 cut(s) 1029
PfeI GAWTC 4 cut(s) 351, 629, 764, 1030
PflFI GACNNNGTC 1 cut(s) 1175
PfoI TCCNGGA 1 cut(s) 683
PinAI ACCGGT 2 cut(s) 156, 1144
PkrI GCNGC 7 cut(s) 221, 347, 496, 670, 1108, 1128, 1414
Ple19I CGATCG 1 cut(s) 826
PleI GAGTC 2 cut(s) 622, 1480
PmaCI CACGTG 3 cut(s) 997, 1314, 1461
PmlI CACGTG 3 cut(s) 997, 1314, 1461
PpsI GAGTC 2 cut(s) 622, 1480
Ppu21I YACGTR 3 cut(s) 997, 1314, 1461
PshAI GACNNNNGTC 1 cut(s) 1461
Psp124BI GAGCTC 3 cut(s) 375, 1023, 1083
Psp1406I AACGTT 3 cut(s) 447, 555, 738
PspCI CACGTG 3 cut(s) 997, 1314, 1461
PspN4I GGNNCC 3 cut(s) 127, 977, 1037
PspPI GGNCC 3 cut(s) 382, 1036, 1358
PstNI CAGNNNCTG 1 cut(s) 497
PsyI GACNNNGTC 1 cut(s) 1175
PteI GCGCGC 1 cut(s) 1003
PvuI CGATCG 1 cut(s) 826
RigI GGCCGGCC 1 cut(s) 936
RsaI GTAC 4 cut(s) 1133, 1143, 1192, 1223
RsaNI GTAC 4 cut(s) 1132, 1142, 1191, 1222
SacI GAGCTC 3 cut(s) 375, 1023, 1083
SalI GTCGAC 2 cut(s) 578, 1148
SaqAI TTAA 3 cut(s) 573, 723, 1013
SatI GCNGC 7 cut(s) 220, 346, 495, 669, 1107, 1127, 1413
Sau3AI GATC 6 cut(s) 640, 743, 823, 838, 1437, 1546
Sau96I GGNCC 3 cut(s) 382, 1036, 1358
SchI GAGTC 2 cut(s) 623, 1480
ScrFI CCNGG 1 cut(s) 685
SduI GDGCHC 5 cut(s) 273, 375, 1023, 1083, 1473
SfaNI GCATC 3 cut(s) 249, 930, 1113
SfcI CTRYAG 1 cut(s) 17
SinI GGWCC 3 cut(s) 382, 1036, 1358
Sse9I AATT 5 cut(s) 137, 586, 634, 784, 813
SstI GAGCTC 3 cut(s) 375, 1023, 1083
StyD4I CCNGG 1 cut(s) 683
StyI CCWWGG 1 cut(s) 1361
TaaI ACNGT 4 cut(s) 21, 778, 831, 1190
TasI AATT 5 cut(s) 137, 586, 634, 784, 813
TauI GCSGC 5 cut(s) 222, 348, 497, 1109, 1129
TfiI GAWTC 4 cut(s) 351, 629, 764, 1030
Tru1I TTAA 3 cut(s) 573, 723, 1013
Tru9I TTAA 3 cut(s) 573, 723, 1013
TscAI CASTG 1 cut(s) 1518
TseFI GTSAC 2 cut(s) 842, 1216
TseI GCWGC 2 cut(s) 668, 1412
Tsp45I GTSAC 2 cut(s) 842, 1216
TspDTI ATGAA 4 cut(s) 637, 1340, 1415, 1511
TspGWI ACGGA 3 cut(s) 224, 1316, 1408
TspRI CASTG 1 cut(s) 1518
Tth111I GACNNNGTC 1 cut(s) 1175
VpaK11BI GGWCC 3 cut(s) 382, 1036, 1358
XapI RAATTY 1 cut(s) 137
XceI RCATGY 2 cut(s) 277, 1093
XcmI CCANNNNNNNNNTGG 1 cut(s) 994
XmiI GTMKAC 2 cut(s) 579, 1149
XmnI GAANNNNTTC 1 cut(s) 1029
ZraI GACGTC 1 cut(s) 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.