RchiOBHm_Chr7g0184751

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008423
Physical Location & Seq
Reverse (-)
5188422 .. 5189218
797 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16484

Sequence Viewer

Length: 618 bp
ATGATCCTCGCCGGCAGGGACACGACGTCTTCTGCTCTATCTTGGTTCTTCTGGATCATATCTTCTAGACCAAACGTCCAGCTCAACATTCTGAATGAGCTCGAAGCCATTCGATCCCGAAACGGCCAAAGTAACGGCGACACTTACGGTTTTGACGAGCTCAGAGACATGCACTATTTGCAGGCAGCGATTATGGAAGCCATGAGACTCTACCCTCCTGTACCGGTGGACACGAAGGCGTGCCTCAACGATGACGTCATGCCGGACGGGACGGTGTTGAGGAAAGGGTGGTTTGTGACGTACCATGCGTATGCCATGGGGAGGATGGAGACGATATGGGGGAAGGACTGCCAGGAGTATGTGCCGGAGAGGTGGCTCGGCGAGGACGGCACGTGTCAGCCGGACAGTGCGTTTCGGTATCCGGTGTTTCACGCCGGGCCGAGAATGTGTCTCGGAAAAGACTTGGCGTTTATTCAGATGAAGTCCATTGCAGCCTCTGTGATACAGAGGTTTGAGTTCGACGTAGAAGATAAGGGCACGTGTCCGGTGCATTTGTTGTCCATGACACTGCGAATTAAAGGTGGACTACCCGTGAGTGTGAGAAAGAGATGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

205

Amino Acids

23.29

Weight (kDa)

6.42

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 182 6e-40 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 29, 258
AclWI GGATC 2 cut(s) 62, 108
AcoI YGGCCR 1 cut(s) 124
AcvI CACGTG 2 cut(s) 393, 540
AcyI GRCGYC 2 cut(s) 26, 255
AfaI GTAC 2 cut(s) 222, 302
AfiI CCNNNNNNNGG 1 cut(s) 321
AflIII ACRYGT 2 cut(s) 392, 539
AgeI ACCGGT 1 cut(s) 223
AhdI GACNNNNNGTC 2 cut(s) 25, 74
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 3 cut(s) 82, 100, 160
AluI AGCT 3 cut(s) 82, 100, 160
Alw21I GWGCWC 2 cut(s) 102, 162
Alw26I GTCTC 4 cut(s) 159, 199, 323, 455
AlwI GGATC 2 cut(s) 62, 108
AlwNI CAGNNNCTG 1 cut(s) 497
AoxI GGCC 2 cut(s) 124, 437
ApeKI GCWGC 2 cut(s) 185, 491
AsiGI ACCGGT 1 cut(s) 223
Asp700I GAANNNNTTC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 437
AsuC2I CCSGG 1 cut(s) 436
BaeGI GKGCMC 1 cut(s) 539
BaeI ACNNNNGTAYC 2 cut(s) 494, 527
BanII GRGCYC 2 cut(s) 102, 162
BbrPI CACGTG 2 cut(s) 393, 540
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 2 cut(s) 102, 162
BbvI GCAGC 2 cut(s) 197, 503
BccI CCATC 1 cut(s) 319
BceAI ACGGC 3 cut(s) 139, 151, 403
BciT130I CCWGG 1 cut(s) 353
BciVI GTATCC 1 cut(s) 429
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 4 cut(s) 159, 199, 323, 455
BfaI CTAG 1 cut(s) 66
BfuI GTATCC 1 cut(s) 429
BisI GCNGC 2 cut(s) 186, 492
BlsI GCNGC 2 cut(s) 187, 493
Bme1390I CCNGG 2 cut(s) 353, 436
BmeRI GACNNNNNGTC 2 cut(s) 25, 74
BmgT120I GGNCC 1 cut(s) 437
BmrFI CCNGG 2 cut(s) 353, 436
BmsI GCATC 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 436
BsaAI YACGTR 2 cut(s) 393, 540
BsaHI GRCGYC 2 cut(s) 26, 255
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 3 cut(s) 223, 421, 544
Bsc4I CCNNNNNNNGG 1 cut(s) 321
Bse118I RCCGGY 2 cut(s) 11, 223
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 1 cut(s) 321
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 175
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 2 cut(s) 197, 503
BshFI GGCC 2 cut(s) 126, 439
BshTI ACCGGT 1 cut(s) 223
BsiHKAI GWGCWC 2 cut(s) 102, 162
BsiSI CCGG 8 cut(s) 12, 224, 263, 365, 401, 422, 435, 545
BslFI GGGAC 2 cut(s) 32, 283
BslI CCNNNNNNNGG 1 cut(s) 321
BsmAI GTCTC 4 cut(s) 159, 199, 323, 455
BsmBI CGTCTC 1 cut(s) 323
BsmFI GGGAC 2 cut(s) 32, 283
BsnI GGCC 2 cut(s) 126, 439
Bsp1286I GDGCHC 3 cut(s) 102, 162, 539
Bsp143I GATC 3 cut(s) 3, 54, 113
Bsp19I CCATGG 1 cut(s) 315
BspANI GGCC 2 cut(s) 126, 439
BspCNI CTCAG 1 cut(s) 174
BspPI GGATC 2 cut(s) 62, 108
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 2 cut(s) 11, 223
BssAI RCCGGY 2 cut(s) 11, 223
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 3 cut(s) 3, 54, 113
BssNI GRCGYC 2 cut(s) 26, 255
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 3 cut(s) 149, 274, 407
BstACI GRCGYC 2 cut(s) 26, 255
BstAPI GCANNNNNTGC 1 cut(s) 178
BstBAI YACGTR 2 cut(s) 393, 540
BstC8I GCNNGC 3 cut(s) 13, 183, 241
BstDEI CTNAG 1 cut(s) 161
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 1 cut(s) 330
BstKTI GATC 3 cut(s) 6, 57, 116
BstMAI GTCTC 4 cut(s) 159, 199, 323, 455
BstMBI GATC 3 cut(s) 3, 54, 113
BstMWI GCNNNNNNNGC 2 cut(s) 178, 387
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 2 cut(s) 351, 434
BstSLI GKGCMC 1 cut(s) 539
BstV1I GCAGC 2 cut(s) 197, 503
BstV2I GAAGAC 1 cut(s) 21
BsuI GTATCC 1 cut(s) 429
BsuRI GGCC 2 cut(s) 126, 439
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 1 cut(s) 330
BtsI GCAGTG 1 cut(s) 566
BtsIMutI CAGTG 2 cut(s) 412, 566
Cac8I GCNNGC 3 cut(s) 13, 183, 241
CaiI CAGNNNCTG 1 cut(s) 497
Cfr10I RCCGGY 2 cut(s) 11, 223
Cfr13I GGNCC 1 cut(s) 437
Csp6I GTAC 2 cut(s) 221, 301
CspAI ACCGGT 1 cut(s) 223
CviAII CATG 6 cut(s) 169, 202, 259, 305, 316, 562
CviQI GTAC 2 cut(s) 221, 301
DdeI CTNAG 1 cut(s) 161
DpnI GATC 3 cut(s) 5, 56, 115
DpnII GATC 3 cut(s) 3, 54, 113
DriI GACNNNNNGTC 2 cut(s) 25, 74
EaeI YGGCCR 1 cut(s) 124
Eam1105I GACNNNNNGTC 2 cut(s) 25, 74
Ecl136II GAGCTC 2 cut(s) 100, 160
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 2 cut(s) 102, 162
Eco53kI GAGCTC 2 cut(s) 100, 160
Eco72I CACGTG 2 cut(s) 393, 540
EcoICRI GAGCTC 2 cut(s) 100, 160
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 315
EcoT22I ATGCAT 1 cut(s) 614
EcoT38I GRGCYC 2 cut(s) 102, 162
ErhI CCWWGG 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 323
FaeI CATG 6 cut(s) 172, 205, 262, 308, 319, 565
FaqI GGGAC 2 cut(s) 32, 283
FatI CATG 6 cut(s) 168, 201, 258, 304, 315, 561
FauNDI CATATG 1 cut(s) 614
Fnu4HI GCNGC 2 cut(s) 186, 492
FokI GGATG 1 cut(s) 337
FriOI GRGCYC 2 cut(s) 102, 162
Fsp4HI GCNGC 2 cut(s) 186, 492
FspBI CTAG 1 cut(s) 66
GluI GCNGC 2 cut(s) 186, 492
HaeIII GGCC 2 cut(s) 126, 439
HapII CCGG 8 cut(s) 12, 224, 263, 365, 401, 422, 435, 545
Hin1I GRCGYC 2 cut(s) 26, 255
Hin1II CATG 6 cut(s) 172, 205, 262, 308, 319, 565
HinfI GANTC 1 cut(s) 207
HpaII CCGG 8 cut(s) 12, 224, 263, 365, 401, 422, 435, 545
Hpy166II GTNNAC 2 cut(s) 229, 584
Hpy188I TCNGA 4 cut(s) 93, 164, 455, 477
Hpy188III TCNNGA 3 cut(s) 52, 66, 117
Hpy8I GTNNAC 2 cut(s) 229, 584
Hpy99I CGWCG 2 cut(s) 28, 524
HpyAV CCTTC 2 cut(s) 229, 337
HpyCH4III ACNGT 3 cut(s) 149, 274, 407
HpyCH4IV ACGT 7 cut(s) 26, 75, 255, 299, 392, 522, 539
HpyCH4V TGCA 5 cut(s) 172, 181, 491, 550, 612
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 387
HpyF3I CTNAG 1 cut(s) 161
HpySE526I ACGT 7 cut(s) 26, 75, 255, 299, 392, 522, 539
Hsp92I GRCGYC 2 cut(s) 26, 255
Hsp92II CATG 6 cut(s) 172, 205, 262, 308, 319, 565
KroI GCCGGC 1 cut(s) 11
KroNI GCCGGC 1 cut(s) 13
Kzo9I GATC 3 cut(s) 3, 54, 113
Lsp1109I GCAGC 2 cut(s) 197, 503
LweI GCATC 1 cut(s) 599
MaeI CTAG 1 cut(s) 66
MaeII ACGT 7 cut(s) 26, 75, 255, 299, 392, 522, 539
MaeIII GTNAC 2 cut(s) 131, 295
MalI GATC 3 cut(s) 5, 56, 115
MboI GATC 3 cut(s) 3, 54, 113
MboII GAAGA 4 cut(s) 21, 40, 54, 539
MhlI GDGCHC 3 cut(s) 102, 162, 539
MluCI AATT 1 cut(s) 573
MlyI GAGTC 1 cut(s) 201
MnlI CCTC 9 cut(s) 17, 225, 254, 273, 315, 363, 376, 501, 505
Mph1103I ATGCAT 1 cut(s) 614
MroNI GCCGGC 1 cut(s) 11
MroXI GAANNNNTTC 1 cut(s) 108
MseI TTAA 1 cut(s) 576
MslI CAYNNNNRTG 1 cut(s) 309
MspI CCGG 8 cut(s) 12, 224, 263, 365, 401, 422, 435, 545
MspR9I CCNGG 2 cut(s) 353, 436
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 178, 387
NaeI GCCGGC 1 cut(s) 13
NciI CCSGG 1 cut(s) 436
NcoI CCATGG 1 cut(s) 315
NdeI CATATG 1 cut(s) 614
NdeII GATC 3 cut(s) 3, 54, 113
NgoMIV GCCGGC 1 cut(s) 11
NlaIII CATG 6 cut(s) 172, 205, 262, 308, 319, 565
NmeAIII GCCGAG 2 cut(s) 357, 465
NmuCI GTSAC 1 cut(s) 295
NsiI ATGCAT 1 cut(s) 614
NspI RCATGY 1 cut(s) 172
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 305
PdiI GCCGGC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 108
PinAI ACCGGT 1 cut(s) 223
PkrI GCNGC 2 cut(s) 187, 493
PleI GAGTC 1 cut(s) 201
PmaCI CACGTG 2 cut(s) 393, 540
PmlI CACGTG 2 cut(s) 393, 540
PpsI GAGTC 1 cut(s) 201
Ppu21I YACGTR 2 cut(s) 393, 540
Psp124BI GAGCTC 2 cut(s) 102, 162
Psp6I CCWGG 1 cut(s) 351
PspCI CACGTG 2 cut(s) 393, 540
PspGI CCWGG 1 cut(s) 351
PspPI GGNCC 1 cut(s) 437
PstNI CAGNNNCTG 1 cut(s) 497
RsaI GTAC 2 cut(s) 222, 302
RsaNI GTAC 2 cut(s) 221, 301
RseI CAYNNNNRTG 1 cut(s) 309
SacI GAGCTC 2 cut(s) 102, 162
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 2 cut(s) 186, 492
Sau3AI GATC 3 cut(s) 3, 54, 113
Sau96I GGNCC 1 cut(s) 437
SchI GAGTC 1 cut(s) 201
ScrFI CCNGG 2 cut(s) 353, 436
SduI GDGCHC 3 cut(s) 102, 162, 539
SfaNI GCATC 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 309
Sse9I AATT 1 cut(s) 573
SspMI CTAG 1 cut(s) 66
SstI GAGCTC 2 cut(s) 102, 162
StyD4I CCNGG 2 cut(s) 351, 434
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 3 cut(s) 149, 274, 407
TaiI ACGT 7 cut(s) 29, 78, 258, 302, 395, 525, 542
TaqI TCGA 3 cut(s) 102, 112, 519
TasI AATT 1 cut(s) 573
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TscAI CASTG 2 cut(s) 412, 573
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 2 cut(s) 185, 491
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 1 cut(s) 494
TspRI CASTG 2 cut(s) 412, 573
XbaI TCTAGA 1 cut(s) 65
XceI RCATGY 1 cut(s) 172
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 1 cut(s) 66
ZraI GACGTC 2 cut(s) 27, 256
Zsp2I ATGCAT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.