Rmu_co8018686.1_g000001

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8018686.1
OG0008423
Physical Location & Seq
Forward (+)
51 .. 538
488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8018686.1_g000001.1.cds

Sequence Viewer

Length: 488 bp
atggagaacaatagctacgaacagcaggatgatttgttatcccggttcatcggaaatgacaacaataactcgccggagtttctccgtgacatcatcataagcatgatcctcgccggcagggacacgacgtcttctgctctatcttggttcttctggatcatatcttctagaccaaacgtccagctcaacattctgaatgagctcgaagccattcgatcccgaaacggcgaaagtaacggcgacacttacggttttgacgagctcagagacatgcactatttgcaggcagcgattatggaagccatgagactctaccctcctgtaccggtggacacgaaggcgtgcctcaacgatgacgtcatgccggacgggacggtgttgaggaaagggtggtttgtgacgtaccatgcgtatgccatggggaggatggagacgatatgggggaaggactgccaggagtatgtgccggagaggtggctcggcgagga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

162

Amino Acids

18.74

Weight (kDa)

4.55

Isoelectric Point (pI)

57.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 131, 360
AclWI GGATC 3 cut(s) 100, 164, 210
AcyI GRCGYC 2 cut(s) 128, 357
AfaI GTAC 2 cut(s) 324, 404
AfiI CCNNNNNNNGG 1 cut(s) 423
AgeI ACCGGT 1 cut(s) 325
AhdI GACNNNNNGTC 2 cut(s) 127, 176
AjnI CCWGG 1 cut(s) 453
AluBI AGCT 4 cut(s) 15, 184, 202, 262
AluI AGCT 4 cut(s) 15, 184, 202, 262
Alw21I GWGCWC 2 cut(s) 204, 264
Alw26I GTCTC 3 cut(s) 261, 301, 425
AlwI GGATC 3 cut(s) 100, 164, 210
ApeKI GCWGC 1 cut(s) 287
AsiGI ACCGGT 1 cut(s) 325
Asp700I GAANNNNTTC 1 cut(s) 210
AsuC2I CCSGG 1 cut(s) 43
BanII GRGCYC 2 cut(s) 204, 264
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 2 cut(s) 204, 264
BbvI GCAGC 1 cut(s) 299
BccI CCATC 1 cut(s) 421
BceAI ACGGC 2 cut(s) 241, 253
BcgI CGANNNNNNTGC 2 cut(s) 91, 125
BciT130I CCWGG 1 cut(s) 455
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 3 cut(s) 261, 301, 425
BfaI CTAG 1 cut(s) 168
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 2 cut(s) 43, 455
BmeRI GACNNNNNGTC 2 cut(s) 127, 176
BmrFI CCNGG 2 cut(s) 43, 455
BpiI GAAGAC 1 cut(s) 123
BpuMI CCSGG 1 cut(s) 43
BsaHI GRCGYC 2 cut(s) 128, 357
BsaJI CCNNGG 1 cut(s) 417
BsaWI WCCGGW 1 cut(s) 325
Bsc4I CCNNNNNNNGG 1 cut(s) 423
Bse118I RCCGGY 2 cut(s) 113, 325
BseBI CCWGG 1 cut(s) 455
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 2 cut(s) 34, 432
BseLI CCNNNNNNNGG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 277
BseXI GCAGC 1 cut(s) 299
BshTI ACCGGT 1 cut(s) 325
BsiHKAI GWGCWC 2 cut(s) 204, 264
BsiSI CCGG 6 cut(s) 43, 74, 114, 326, 365, 467
BslFI GGGAC 2 cut(s) 134, 385
BslI CCNNNNNNNGG 1 cut(s) 423
BsmAI GTCTC 3 cut(s) 261, 301, 425
BsmBI CGTCTC 1 cut(s) 425
BsmFI GGGAC 2 cut(s) 134, 385
Bsp1286I GDGCHC 2 cut(s) 204, 264
Bsp143I GATC 3 cut(s) 105, 156, 215
Bsp19I CCATGG 1 cut(s) 417
BspCNI CTCAG 1 cut(s) 276
BspPI GGATC 3 cut(s) 100, 164, 210
BsrFI RCCGGY 2 cut(s) 113, 325
BssAI RCCGGY 2 cut(s) 113, 325
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 3 cut(s) 105, 156, 215
BssNI GRCGYC 2 cut(s) 128, 357
BssT1I CCWWGG 1 cut(s) 417
Bst2UI CCWGG 1 cut(s) 455
Bst4CI ACNGT 2 cut(s) 251, 376
BstACI GRCGYC 2 cut(s) 128, 357
BstAPI GCANNNNNTGC 1 cut(s) 280
BstC8I GCNNGC 3 cut(s) 115, 285, 343
BstDEI CTNAG 1 cut(s) 263
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 2 cut(s) 34, 432
BstKTI GATC 3 cut(s) 108, 159, 218
BstMAI GTCTC 3 cut(s) 261, 301, 425
BstMBI GATC 3 cut(s) 105, 156, 215
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 1 cut(s) 455
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 2 cut(s) 41, 453
BstV1I GCAGC 1 cut(s) 299
BstV2I GAAGAC 1 cut(s) 123
BtgI CCRYGG 1 cut(s) 417
BtsCI GGATG 2 cut(s) 34, 432
Cac8I GCNNGC 3 cut(s) 115, 285, 343
Cfr10I RCCGGY 2 cut(s) 113, 325
Csp6I GTAC 2 cut(s) 323, 403
CspAI ACCGGT 1 cut(s) 325
CviAII CATG 6 cut(s) 103, 271, 304, 361, 407, 418
CviJI RGCY 7 cut(s) 15, 184, 202, 209, 262, 302, 478
CviKI_1 RGCY 7 cut(s) 15, 184, 202, 209, 262, 302, 478
CviQI GTAC 2 cut(s) 323, 403
DdeI CTNAG 1 cut(s) 263
DpnI GATC 3 cut(s) 107, 158, 217
DpnII GATC 3 cut(s) 105, 156, 215
DriI GACNNNNNGTC 2 cut(s) 127, 176
Eam1105I GACNNNNNGTC 2 cut(s) 127, 176
Ecl136II GAGCTC 2 cut(s) 202, 262
Eco130I CCWWGG 1 cut(s) 417
Eco24I GRGCYC 2 cut(s) 204, 264
Eco53kI GAGCTC 2 cut(s) 202, 262
EcoICRI GAGCTC 2 cut(s) 202, 262
EcoRII CCWGG 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 417
EcoT38I GRGCYC 2 cut(s) 204, 264
ErhI CCWWGG 1 cut(s) 417
Esp3I CGTCTC 1 cut(s) 425
FaeI CATG 6 cut(s) 106, 274, 307, 364, 410, 421
FaqI GGGAC 2 cut(s) 134, 385
FatI CATG 6 cut(s) 102, 270, 303, 360, 406, 417
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 2 cut(s) 41, 439
FriOI GRGCYC 2 cut(s) 204, 264
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 1 cut(s) 168
GluI GCNGC 1 cut(s) 288
HapII CCGG 6 cut(s) 43, 74, 114, 326, 365, 467
Hin1I GRCGYC 2 cut(s) 128, 357
Hin1II CATG 6 cut(s) 106, 274, 307, 364, 410, 421
HinfI GANTC 1 cut(s) 309
HpaII CCGG 6 cut(s) 43, 74, 114, 326, 365, 467
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 53, 195, 266
Hpy188III TCNNGA 3 cut(s) 154, 168, 219
Hpy8I GTNNAC 1 cut(s) 331
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 2 cut(s) 331, 439
HpyCH4III ACNGT 2 cut(s) 251, 376
HpyCH4IV ACGT 4 cut(s) 128, 177, 357, 401
HpyCH4V TGCA 2 cut(s) 274, 283
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 1 cut(s) 263
HpySE526I ACGT 4 cut(s) 128, 177, 357, 401
Hsp92I GRCGYC 2 cut(s) 128, 357
Hsp92II CATG 6 cut(s) 106, 274, 307, 364, 410, 421
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
Kzo9I GATC 3 cut(s) 105, 156, 215
Lsp1109I GCAGC 1 cut(s) 299
MaeI CTAG 1 cut(s) 168
MaeII ACGT 4 cut(s) 128, 177, 357, 401
MaeIII GTNAC 3 cut(s) 86, 233, 397
MalI GATC 3 cut(s) 107, 158, 217
MboI GATC 3 cut(s) 105, 156, 215
MboII GAAGA 3 cut(s) 123, 142, 156
MhlI GDGCHC 2 cut(s) 204, 264
MlyI GAGTC 1 cut(s) 303
MnlI CCTC 7 cut(s) 119, 327, 356, 375, 417, 465, 478
MroNI GCCGGC 1 cut(s) 113
MroXI GAANNNNTTC 1 cut(s) 210
MslI CAYNNNNRTG 2 cut(s) 101, 411
MspI CCGG 6 cut(s) 43, 74, 114, 326, 365, 467
MspR9I CCNGG 2 cut(s) 43, 455
MvaI CCWGG 1 cut(s) 455
MwoI GCNNNNNNNGC 1 cut(s) 280
NaeI GCCGGC 1 cut(s) 115
NciI CCSGG 1 cut(s) 43
NcoI CCATGG 1 cut(s) 417
NdeII GATC 3 cut(s) 105, 156, 215
NgoMIV GCCGGC 1 cut(s) 113
NlaIII CATG 6 cut(s) 106, 274, 307, 364, 410, 421
NmeAIII GCCGAG 1 cut(s) 459
NmuCI GTSAC 2 cut(s) 86, 397
NspI RCATGY 1 cut(s) 274
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 407
PdiI GCCGGC 1 cut(s) 115
PdmI GAANNNNTTC 1 cut(s) 210
PinAI ACCGGT 1 cut(s) 325
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
Psp124BI GAGCTC 2 cut(s) 204, 264
Psp6I CCWGG 1 cut(s) 453
PspGI CCWGG 1 cut(s) 453
PsrI GAACNNNNNNTAC 1 cut(s) 31
RsaI GTAC 2 cut(s) 324, 404
RsaNI GTAC 2 cut(s) 323, 403
RseI CAYNNNNRTG 2 cut(s) 101, 411
SacI GAGCTC 2 cut(s) 204, 264
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 3 cut(s) 105, 156, 215
SchI GAGTC 1 cut(s) 303
ScrFI CCNGG 2 cut(s) 43, 455
SduI GDGCHC 2 cut(s) 204, 264
SetI ASST 9 cut(s) 17, 131, 180, 186, 204, 264, 360, 404, 476
SmiMI CAYNNNNRTG 2 cut(s) 101, 411
SspMI CTAG 1 cut(s) 168
SstI GAGCTC 2 cut(s) 204, 264
StyD4I CCNGG 2 cut(s) 41, 453
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 2 cut(s) 251, 376
TaiI ACGT 4 cut(s) 131, 180, 360, 404
TaqI TCGA 2 cut(s) 204, 214
TseFI GTSAC 2 cut(s) 86, 397
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 2 cut(s) 86, 397
TspDTI ATGAA 1 cut(s) 37
TspGWI ACGGA 1 cut(s) 74
XbaI TCTAGA 1 cut(s) 167
XceI RCATGY 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 1 cut(s) 168
ZraI GACGTC 2 cut(s) 129, 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.