MD14G1222800.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008423
Physical Location & Seq
Reverse (-)
30424253 .. 30425815
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1222800.v1.1.491

Sequence Viewer

Length: 1563 bp
ATGGAGCTCTTCTCCCTGCAATCTTTCCTCCTCCTCCTTCTCTCCCTCTCCTTCTACATCTACCACCACTTCTTCGCTTCGTCGAAGACGTACAAACACACCGGCTTCAAAAGCTACCTGATTCTCGGAACCCTACCGGAATTCCTAAAAAACCGCCACCGGTTCCTCGATTGGACAACCGAAATCCTCACCAGCTGCCCCACCAGCACCGCCATCATCAGCCGCCCGGTAAAAATCCGCGGCATCATCACCGCCAACCCTGCCAACGTCGAGCACATGCTGAAAACCAACTTCGAGAACTACCCGAAAGGGGAAAGGTTCATATCTCTTATCCAAGACTTTCTCGGCGGCGGAATCTTCAACTCCGACGGCGAGCTCTGGAAGGTTCAGAGGAAGACCGCCAGCTACGCCTTCAACACGAAATCGCTCCGGAACTTCGTTATGGAAAACGTTAGGGTTGAGATTGAGTCGAGGTTAATTCCGTCTCTGGACACAGCCGCCGAAACAGGACGCCGTTTGGACTTGCAAGATGTTTTGGAGCGCTTTGCTTTCGACAACGTTTGCAAATTGGCTTTTAATGTGGACCCGAATTGTCTCGGAAGCGGCGGCGGCGGAAAATCTGAGTCCACGGACTTCGATTCCAATCCCAATCCGGATCGGTTCATGCAGGCATTTGAAGAAGCGGCCACACTTATCTCTGGGAGATTTTTGTACGCGTTTCAGTTTATTCAAAAGATAAAGACGTTTTTGAACGTTGGATCAGAGCGGAGGTTAAAAGAATCAATCTCGACAGTCCATAACTTCGCGGATAAGATAATAAAGTCGAGAATTAGCAACGAACTGTCGGAGGATCGTCACGATTTGTTGTCCTTGTTTATTGGTATTCCCGAAAACAACTCGCCGGATTTTCTCCGAGATATCGTGATAAGCATCATTCTCGCCGGCCGAGACACGACATCATCGGCTCTGACGTGGTTCTTCTGGCTCCTGTCGTCCAGACCGCACGTGGAGCACGCCATTTTAAAGGAGCTGGAAATGATTCGGGTACGAAACGGGAAACGTATCGGCGATGTTTATGATTTGGACGAGCTCCGAGACATGCACTATTTGCACGCGGCGATTTCAGAGGCGATGCGGCTGTACCCTCCCGTACCGGTCGACACGAAGGCGTGTCTAAACGATGATGTCTTGCCGGACGGGACAGCCGTGGGGAAGGGGTGGTTCGTGACGTACCATGCTTACGCGATGGGGCGGATGGAGAAGATATGGGGGACTACCTGCCACGAGTATTTGCCGGAGCGGTGGCTGACCGAGGACGGCACATGTCGGCAGGAGAGTGCTTTCCGGTATCCGATATTTCATGCTGGGCCAAGGATGTGTCTGGGGAAGGATTTGGCGTATATTCAGATGAAGTCGATTGCGGCATCGGTGATGGAGAGGTTTGTGATCGGCGTACAGGATAGGGATACGTGTCCTAAGCATCTGTTGTCCTTGACTCTCAGGATGAAAGGTGGGCTGCCCGTGAGTGTGAGGAGAAGGAGATTTGTAGCTGATGACCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

521

Amino Acids

59.43

Weight (kDa)

8.3

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 83 - 493 2.2e-59 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1286
AccBSI CCGCTC 2 cut(s) 766, 1300
AccI GTMKAC 1 cut(s) 1158
AccII CGCG 5 cut(s) 240, 716, 806, 1115, 1244
AccIII TCCGGA 2 cut(s) 429, 652
AclI AACGTT 3 cut(s) 450, 558, 753
AclWI GGATC 3 cut(s) 663, 766, 858
AcoI YGGCCR 2 cut(s) 684, 943
AcsI RAATTY 1 cut(s) 140
AcvI CACGTG 1 cut(s) 1006
AcyI GRCGYC 1 cut(s) 511
AfaI GTAC 7 cut(s) 92, 713, 1047, 1142, 1152, 1232, 1455
AfeI AGCGCT 1 cut(s) 542
AfiI CCNNNNNNNGG 1 cut(s) 310
AflIII ACRYGT 3 cut(s) 714, 1322, 1469
AgeI ACCGGT 3 cut(s) 159, 1153, 1557
AgsI TTSAA 6 cut(s) 109, 361, 415, 677, 731, 751
AjiI CACGTC 1 cut(s) 972
AluBI AGCT 8 cut(s) 7, 114, 195, 376, 405, 1030, 1090, 1550
AluI AGCT 8 cut(s) 7, 114, 195, 376, 405, 1030, 1090, 1550
Alw21I GWGCWC 5 cut(s) 9, 276, 378, 1014, 1092
Alw26I GTCTC 4 cut(s) 489, 599, 942, 1089
AlwI GGATC 3 cut(s) 663, 766, 858
Aor13HI TCCGGA 2 cut(s) 429, 652
Aor51HI AGCGCT 1 cut(s) 542
AoxI GGCC 3 cut(s) 684, 943, 1367
ApeKI GCWGC 2 cut(s) 195, 1516
ApoI RAATTY 1 cut(s) 140
AsiGI ACCGGT 3 cut(s) 159, 1153, 1557
Asp700I GAANNNNTTC 1 cut(s) 1038
AspLEI GCGC 1 cut(s) 543
AspS9I GGNCC 2 cut(s) 583, 1367
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 3 cut(s) 181, 241, 1441
AvaII GGWCC 1 cut(s) 583
BanII GRGCYC 3 cut(s) 9, 378, 1092
BauI CACGAG 1 cut(s) 1283
BbrPI CACGTG 1 cut(s) 1006
BbsI GAAGAC 2 cut(s) 92, 401
Bbv12I GWGCWC 5 cut(s) 9, 276, 378, 1014, 1092
BbvI GCAGC 2 cut(s) 182, 1503
BccI CCATC 4 cut(s) 221, 1240, 1249, 1426
BceAI ACGGC 4 cut(s) 385, 498, 1190, 1333
BcgI CGANNNNNNTGC 2 cut(s) 919, 953
BciVI GTATCC 2 cut(s) 1359, 1459
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 4 cut(s) 489, 599, 942, 1089
BfoI RGCGCY 1 cut(s) 544
BfuAI ACCTGC 1 cut(s) 1286
BfuI GTATCC 2 cut(s) 1359, 1459
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 1 cut(s) 583
BmgBI CACGTC 1 cut(s) 972
BmgT120I GGNCC 2 cut(s) 583, 1367
BmiI GGNNCC 4 cut(s) 130, 164, 585, 986
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 5 cut(s) 252, 939, 1122, 1433, 1489
BpiI GAAGAC 2 cut(s) 92, 401
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 1476
BpuMI CCSGG 1 cut(s) 227
BsaAI YACGTR 2 cut(s) 1006, 1470
BsaBI GATNNNNATC 2 cut(s) 642, 929
BsaHI GRCGYC 1 cut(s) 511
BsaJI CCNNGG 5 cut(s) 238, 627, 1206, 1311, 1370
BsaWI WCCGGW 7 cut(s) 136, 159, 429, 652, 1153, 1344, 1557
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse118I RCCGGY 5 cut(s) 101, 159, 941, 1153, 1557
Bse8I GATNNNNATC 2 cut(s) 642, 929
BseAI TCCGGA 2 cut(s) 429, 652
BseDI CCNNGG 5 cut(s) 238, 627, 1206, 1311, 1370
BseGI GGATG 3 cut(s) 1260, 1380, 1509
BseJI GATNNNNATC 2 cut(s) 642, 929
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMII CTCAG 2 cut(s) 612, 1513
BseRI GAGGAG 3 cut(s) 20, 23, 1546
BseX3I CGGCCG 1 cut(s) 943
BseXI GCAGC 2 cut(s) 182, 1503
BseYI CCCAGC 1 cut(s) 1364
Bsh1236I CGCG 5 cut(s) 240, 716, 806, 1115, 1244
Bsh1285I CGRYCG 2 cut(s) 946, 1158
BshFI GGCC 3 cut(s) 686, 945, 1369
BshTI ACCGGT 3 cut(s) 159, 1153, 1557
BsiEI CGRYCG 2 cut(s) 946, 1158
BsiHKAI GWGCWC 5 cut(s) 9, 276, 378, 1014, 1092
BslFI GGGAC 2 cut(s) 1213, 1285
BslI CCNNNNNNNGG 1 cut(s) 310
BsmAI GTCTC 4 cut(s) 489, 599, 942, 1089
BsmBI CGTCTC 1 cut(s) 489
BsmFI GGGAC 2 cut(s) 1213, 1285
BsnI GGCC 3 cut(s) 686, 945, 1369
Bsp1286I GDGCHC 5 cut(s) 9, 276, 378, 1014, 1092
Bsp13I TCCGGA 2 cut(s) 429, 652
Bsp143I GATC 4 cut(s) 655, 758, 850, 1446
BspANI GGCC 3 cut(s) 686, 945, 1369
BspCNI CTCAG 2 cut(s) 613, 1512
BspEI TCCGGA 2 cut(s) 429, 652
BspFNI CGCG 5 cut(s) 240, 716, 806, 1115, 1244
BspLI GGNNCC 4 cut(s) 130, 164, 585, 986
BspMI ACCTGC 1 cut(s) 1286
BspPI GGATC 3 cut(s) 663, 766, 858
BspQI GCTCTTC 1 cut(s) 14
BsrBI CCGCTC 2 cut(s) 766, 1300
BsrFI RCCGGY 5 cut(s) 101, 159, 941, 1153, 1557
BssAI RCCGGY 5 cut(s) 101, 159, 941, 1153, 1557
BssECI CCNNGG 5 cut(s) 238, 627, 1206, 1311, 1370
BssMI GATC 4 cut(s) 655, 758, 850, 1446
BssNI GRCGYC 1 cut(s) 511
BssSI CACGAG 1 cut(s) 1283
BssT1I CCWWGG 1 cut(s) 1370
Bst2BI CACGAG 1 cut(s) 1283
Bst4CI ACNGT 2 cut(s) 793, 843
Bst6I CTCTTC 1 cut(s) 14
BstACI GRCGYC 1 cut(s) 511
BstAPI GCANNNNNTGC 1 cut(s) 1108
BstBAI YACGTR 2 cut(s) 1006, 1470
BstC8I GCNNGC 6 cut(s) 374, 403, 669, 943, 1014, 1113
BstDEI CTNAG 3 cut(s) 621, 1476, 1499
BstDSI CCRYGG 3 cut(s) 238, 627, 1206
BstF5I GGATG 3 cut(s) 1260, 1380, 1509
BstFNI CGCG 5 cut(s) 240, 716, 806, 1115, 1244
BstH2I RGCGCY 1 cut(s) 544
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 4 cut(s) 658, 761, 853, 1449
BstMAI GTCTC 4 cut(s) 489, 599, 942, 1089
BstMBI GATC 4 cut(s) 655, 758, 850, 1446
BstMCI CGRYCG 2 cut(s) 946, 1158
BstMWI GCNNNNNNNGC 7 cut(s) 111, 204, 260, 407, 609, 1009, 1108
BstNSI RCATGY 3 cut(s) 280, 1102, 1326
BstSCI CCNGG 1 cut(s) 225
BstUI CGCG 5 cut(s) 240, 716, 806, 1115, 1244
BstV1I GCAGC 2 cut(s) 182, 1503
BstV2I GAAGAC 2 cut(s) 92, 401
BstZI CGGCCG 1 cut(s) 943
BsuI GTATCC 2 cut(s) 1359, 1459
BsuRI GGCC 3 cut(s) 686, 945, 1369
BtgI CCRYGG 3 cut(s) 238, 627, 1206
BtgZI GCGATG 3 cut(s) 1083, 1145, 1259
BtrI CACGTC 1 cut(s) 972
BtsCI GGATG 3 cut(s) 1260, 1380, 1509
BveI ACCTGC 1 cut(s) 1286
Cac8I GCNNGC 6 cut(s) 374, 403, 669, 943, 1014, 1113
CfoI GCGC 1 cut(s) 543
Cfr10I RCCGGY 5 cut(s) 101, 159, 941, 1153, 1557
Cfr13I GGNCC 2 cut(s) 583, 1367
Cfr42I CCGCGG 1 cut(s) 241
CseI GACGC 1 cut(s) 519
Csp6I GTAC 7 cut(s) 91, 712, 1046, 1141, 1151, 1231, 1454
CspAI ACCGGT 3 cut(s) 159, 1153, 1557
CviAII CATG 6 cut(s) 277, 664, 1099, 1235, 1323, 1361
CviQI GTAC 7 cut(s) 91, 712, 1046, 1141, 1151, 1231, 1454
DdeI CTNAG 3 cut(s) 621, 1476, 1499
DpnI GATC 4 cut(s) 657, 760, 852, 1448
DpnII GATC 4 cut(s) 655, 758, 850, 1446
DraI TTTAAA 1 cut(s) 1023
EaeI YGGCCR 2 cut(s) 684, 943
EagI CGGCCG 1 cut(s) 943
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
EciI GGCGGA 3 cut(s) 366, 627, 1267
Ecl136II GAGCTC 3 cut(s) 7, 376, 1090
EclXI CGGCCG 1 cut(s) 943
Eco130I CCWWGG 1 cut(s) 1370
Eco24I GRGCYC 3 cut(s) 9, 378, 1092
Eco32I GATATC 1 cut(s) 919
Eco47I GGWCC 1 cut(s) 583
Eco47III AGCGCT 1 cut(s) 542
Eco52I CGGCCG 1 cut(s) 943
Eco53kI GAGCTC 3 cut(s) 7, 376, 1090
Eco72I CACGTG 1 cut(s) 1006
EcoICRI GAGCTC 3 cut(s) 7, 376, 1090
EcoRI GAATTC 1 cut(s) 140
EcoRV GATATC 1 cut(s) 919
EcoT14I CCWWGG 1 cut(s) 1370
EcoT38I GRGCYC 3 cut(s) 9, 378, 1092
ErhI CCWWGG 1 cut(s) 1370
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 6 cut(s) 280, 667, 1102, 1238, 1326, 1364
FaqI GGGAC 2 cut(s) 1213, 1285
FatI CATG 6 cut(s) 276, 663, 1098, 1234, 1322, 1360
FblI GTMKAC 1 cut(s) 1158
FokI GGATG 3 cut(s) 1267, 1387, 1516
FriOI GRGCYC 3 cut(s) 9, 378, 1092
GlaI GCGC 1 cut(s) 542
GsaI CCCAGC 1 cut(s) 1368
HaeII RGCGCY 1 cut(s) 544
HaeIII GGCC 3 cut(s) 686, 945, 1369
HgaI GACGC 1 cut(s) 519
HhaI GCGC 1 cut(s) 543
Hin1I GRCGYC 1 cut(s) 511
Hin1II CATG 6 cut(s) 280, 667, 1102, 1238, 1326, 1364
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HincII GTYRAC 1 cut(s) 1159
HindII GTYRAC 1 cut(s) 1159
HinfI GANTC 8 cut(s) 121, 354, 467, 623, 638, 779, 1039, 1495
HphI GGTGA 3 cut(s) 181, 241, 1441
Hpy166II GTNNAC 3 cut(s) 583, 627, 1159
Hpy8I GTNNAC 3 cut(s) 583, 627, 1159
Hpy99I CGWCG 3 cut(s) 85, 272, 371
HpyAV CCTTC 8 cut(s) 47, 61, 376, 421, 1159, 1207, 1381, 1530
HpyCH4III ACNGT 2 cut(s) 793, 843
HpyCH4V TGCA 6 cut(s) 19, 526, 564, 667, 1102, 1111
HpyF10VI GCNNNNNNNGC 7 cut(s) 111, 204, 260, 407, 609, 1009, 1108
HpyF3I CTNAG 3 cut(s) 621, 1476, 1499
Hsp92I GRCGYC 1 cut(s) 511
Hsp92II CATG 6 cut(s) 280, 667, 1102, 1238, 1326, 1364
HspAI GCGC 1 cut(s) 541
Kpn2I TCCGGA 2 cut(s) 429, 652
KroI GCCGGC 1 cut(s) 941
KroNI GCCGGC 1 cut(s) 943
KspI CCGCGG 1 cut(s) 241
Kzo9I GATC 4 cut(s) 655, 758, 850, 1446
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 8 cut(s) 4, 432, 538, 990, 1009, 1027, 1095, 1297
Lsp1109I GCAGC 2 cut(s) 182, 1503
LweI GCATC 5 cut(s) 252, 939, 1122, 1433, 1489
MaeIII GTNAC 2 cut(s) 854, 1225
MalI GATC 4 cut(s) 657, 760, 852, 1448
MbiI CCGCTC 2 cut(s) 766, 1300
MboI GATC 4 cut(s) 655, 758, 850, 1446
MboII GAAGA 7 cut(s) 64, 97, 349, 406, 689, 970, 1273
MhlI GDGCHC 5 cut(s) 9, 276, 378, 1014, 1092
MluCI AATT 5 cut(s) 140, 477, 566, 589, 828
MluI ACGCGT 1 cut(s) 714
MlyI GAGTC 3 cut(s) 476, 632, 1489
MmeI TCCRAC 3 cut(s) 390, 736, 825
MroI TCCGGA 2 cut(s) 429, 652
MroNI GCCGGC 1 cut(s) 941
MroXI GAANNNNTTC 1 cut(s) 1038
MseI TTAA 4 cut(s) 476, 576, 773, 1022
MspA1I CMGCKG 2 cut(s) 195, 240
MspR9I CCNGG 1 cut(s) 227
MvnI CGCG 5 cut(s) 240, 716, 806, 1115, 1244
MwoI GCNNNNNNNGC 7 cut(s) 111, 204, 260, 407, 609, 1009, 1108
NaeI GCCGGC 1 cut(s) 943
NciI CCSGG 1 cut(s) 227
NdeII GATC 4 cut(s) 655, 758, 850, 1446
NgoMIV GCCGGC 1 cut(s) 941
NlaIII CATG 6 cut(s) 280, 667, 1102, 1238, 1326, 1364
NlaIV GGNNCC 4 cut(s) 130, 164, 585, 986
NmeAIII GCCGAG 2 cut(s) 324, 971
NmuCI GTSAC 2 cut(s) 854, 1225
NspI RCATGY 3 cut(s) 280, 1102, 1326
PciI ACATGT 1 cut(s) 1322
PciSI GCTCTTC 1 cut(s) 14
PcsI WCGNNNNNNNCGW 3 cut(s) 86, 968, 1203
PdiI GCCGGC 1 cut(s) 943
PdmI GAANNNNTTC 1 cut(s) 1038
PfeI GAWTC 5 cut(s) 121, 354, 638, 779, 1039
PinAI ACCGGT 3 cut(s) 159, 1153, 1557
PleI GAGTC 3 cut(s) 475, 631, 1489
PmaCI CACGTG 1 cut(s) 1006
PmlI CACGTG 1 cut(s) 1006
PpsI GAGTC 3 cut(s) 475, 631, 1489
Ppu21I YACGTR 2 cut(s) 1006, 1470
PscI ACATGT 1 cut(s) 1322
Psp124BI GAGCTC 3 cut(s) 9, 378, 1092
Psp1406I AACGTT 3 cut(s) 450, 558, 753
PspCI CACGTG 1 cut(s) 1006
PspFI CCCAGC 1 cut(s) 1364
PspN4I GGNNCC 4 cut(s) 130, 164, 585, 986
PspPI GGNCC 2 cut(s) 583, 1367
PvuII CAGCTG 1 cut(s) 195
RsaI GTAC 7 cut(s) 92, 713, 1047, 1142, 1152, 1232, 1455
RsaNI GTAC 7 cut(s) 91, 712, 1046, 1141, 1151, 1231, 1454
SacI GAGCTC 3 cut(s) 9, 378, 1092
SacII CCGCGG 1 cut(s) 241
SalI GTCGAC 1 cut(s) 1157
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 4 cut(s) 476, 576, 773, 1022
Sau3AI GATC 4 cut(s) 655, 758, 850, 1446
Sau96I GGNCC 2 cut(s) 583, 1367
SchI GAGTC 3 cut(s) 476, 632, 1489
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 5 cut(s) 9, 276, 378, 1014, 1092
SfaNI GCATC 5 cut(s) 252, 939, 1122, 1433, 1489
Sfr303I CCGCGG 1 cut(s) 241
SgrBI CCGCGG 1 cut(s) 241
SinI GGWCC 1 cut(s) 583
Sse9I AATT 5 cut(s) 140, 477, 566, 589, 828
SstI GAGCTC 3 cut(s) 9, 378, 1092
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 1370
TaaI ACNGT 2 cut(s) 793, 843
TaqII GACCGA 1 cut(s) 1325
TasI AATT 5 cut(s) 140, 477, 566, 589, 828
TfiI GAWTC 5 cut(s) 121, 354, 638, 779, 1039
Tru1I TTAA 4 cut(s) 476, 576, 773, 1022
Tru9I TTAA 4 cut(s) 476, 576, 773, 1022
TseFI GTSAC 2 cut(s) 854, 1225
TseI GCWGC 2 cut(s) 195, 1516
Tsp45I GTSAC 2 cut(s) 854, 1225
TspDTI ATGAA 5 cut(s) 310, 652, 1349, 1424, 1520
TspGWI ACGGA 2 cut(s) 471, 644
VpaK11BI GGWCC 1 cut(s) 583
XapI RAATTY 1 cut(s) 140
XceI RCATGY 3 cut(s) 280, 1102, 1326
XcmI CCANNNNNNNNNTGG 1 cut(s) 1003
XmiI GTMKAC 1 cut(s) 1158
XmnI GAANNNNTTC 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.