AT1G38065

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008279
Physical Location & Seq
Reverse (-)
14289533 .. 14292098
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G38065.2

Sequence Viewer

Length: 1413 bp
ATGCCAAAACCTTGTCCTAATTCAACAGTTTCATCACATTTTGGTCTGAACAGAGGTATTAATGTGTTTGATGAAATATTTTCAGAATCATCTGCTCTTGCTCCTAGTAGTGGTTACTTAATCGTGGAAGCAAATGGCGGTCTTAATCAGCAGCGTTCTGCGATTTGCAATGCTGTGGCTGTAGCCGGGCTTCTTAACGCAGTTTTAGTCATACCGCGGTTTGAGTTTCACGCTATTTGGAAGGATTCAAGCAACTTTGGTGATATCTATGACGAAGATCACTTCATATCAAGCCTTGAAGGTTATGTGAAAATAGTGAGAGATGTTCCTGATGAGATAATGACTCGTTTTAGTTACAATGTTTCAAGTATTCCTACTATTCGTGTTCAAGCTTGGGCTACTGTGAACTACTATAACGGGGAAGTTTATCCGGTTTTAAAGGAACATGGGGTTATTCGGATAACTCCTTTCGCAAATAGATTGGCGATGAGTGTTCCACCGTATATACAACTTTTGAGGTGCATAGCTAATTACAAAGCATTGAAGTTTTCTTCACCTATATCAACACTAGCTGAGAAGTTAGTCGATCGGATGGTTGAGAAAAGCTCGGCGACCGGAGGAAAGTATGTCTCGGTCCACCTTCGATTTGAGGAGGACATGGTGGCATTCTCATGTTGTCTCTACGAAGGTGGAAGAGCTGAGAAATCTGAAATGGATGTGATTAGACAGAAAAGTTGGAAAGGAAAGTTCAAACGAAGAGATCGTGTCATTAGGCCCGACCTTAACCGAGTTAACGGAAAATGTCCCCTTACCCCATTAGAGGTCGGTATGATGCTCCGTGGTATGGGATTTGATAACAACACTTCGATATATTTGGCCTCGGGAAGAATCTACCAACCTGAGAAACATTTGGCTCCTCTTCAAGAAATGTTTCCTCGTCTCTACACAAAAGAGTCTCTTGCAACTCCAGAGGAGCTTGCCCCATTTCAGGGATATTCCTCCAGAATGGCTGCTTTGGACTACACAGTGAGTTTGCTAAGTGAAGTCTTTGTGACAACTCAAGGTGGAAACTTTCCTCATTTTTTGATGGGCCATCGAAGATTCCTCTTCGGTGGACATGCAAAAACTGTCATACCCGACAAACCCAAACTCGTTCTCCTTCTTCAGGACATGGAAATGAGGTGGGAAGTTTTCAAAAAGGAGATGAAACTGATGCTAGGGGAAAGCGATAGAAAGGGAGTGATGGTACCGAGAGTCAGGAAGATCAACCGGAAAACTTCGATTTACACATACCCGTTACCCGAATGCGAATGCATCTTCCATTTGTCTTCCAATTTTTCAAACACCGGAAACATTTTGAGCCTTGGAGCTCTTCATCCATCTTCCAACCTCATAAGTTCTGCAAGACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

470

Amino Acids

52.93

Weight (kDa)

9.07

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 29 - 358 8e-101 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1246
AccB1I GGYRCC 1 cut(s) 1246
AccII CGCG 1 cut(s) 217
AciI CCGC 3 cut(s) 138, 215, 217
AcuI CTGAAG 1 cut(s) 1148
AfaI GTAC 1 cut(s) 1248
AfiI CCNNNNNNNGG 6 cut(s) 110, 820, 844, 988, 989, 1165
AluBI AGCT 7 cut(s) 392, 527, 572, 606, 698, 976, 1370
AluI AGCT 7 cut(s) 392, 527, 572, 606, 698, 976, 1370
Alw21I GWGCWC 1 cut(s) 1372
Alw26I GTCTC 4 cut(s) 634, 683, 944, 960
Ama87I CYCGRG 1 cut(s) 880
AoxI GGCC 3 cut(s) 773, 876, 1090
ApeKI GCWGC 2 cut(s) 151, 1010
AseI ATTAAT 1 cut(s) 60
Asp700I GAANNNNTTC 1 cut(s) 930
Asp718I GGTACC 1 cut(s) 1246
AspS9I GGNCC 3 cut(s) 634, 774, 1090
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 2 cut(s) 272, 546
AvaI CYCGRG 1 cut(s) 880
AvaII GGWCC 1 cut(s) 634
BanI GGYRCC 1 cut(s) 1246
BanII GRGCYC 1 cut(s) 1372
BarI GAAGNNNNNNTAC 2 cut(s) 174, 206
BbsI GAAGAC 1 cut(s) 1320
Bbv12I GWGCWC 1 cut(s) 1372
BbvI GCAGC 2 cut(s) 163, 997
BccI CCATC 5 cut(s) 586, 1081, 1101, 1237, 1387
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 4 cut(s) 634, 683, 944, 960
BfaI CTAG 3 cut(s) 105, 569, 1217
BfmI CTRYAG 2 cut(s) 180, 1409
BisI GCNGC 2 cut(s) 152, 1011
BlsI GCNGC 2 cut(s) 153, 1012
Bme1390I CCNGG 1 cut(s) 187
Bme18I GGWCC 1 cut(s) 634
BmeT110I CYCGRG 1 cut(s) 880
BmgT120I GGNCC 3 cut(s) 634, 774, 1090
BmiI GGNNCC 2 cut(s) 915, 1248
BmrFI CCNGG 1 cut(s) 187
BmsI GCATC 3 cut(s) 822, 1203, 1323
BpiI GAAGAC 1 cut(s) 1320
BplI GAGNNNNNCTC 2 cut(s) 590, 622
BpmI CTGGAG 2 cut(s) 951, 985
BpuEI CTTGAG 1 cut(s) 1044
BpuMI CCSGG 1 cut(s) 187
BsaJI CCNNGG 4 cut(s) 215, 838, 879, 1363
BsaWI WCCGGW 4 cut(s) 430, 614, 1269, 1346
BsaXI ACNNNNNCTCC 4 cut(s) 1140, 1170, 1230, 1260
Bsc4I CCNNNNNNNGG 6 cut(s) 110, 820, 844, 988, 989, 1165
Bse3DI GCAATG 1 cut(s) 175
BseDI CCNNGG 4 cut(s) 215, 838, 879, 1363
BseGI GGATG 3 cut(s) 597, 721, 1375
BseLI CCNNNNNNNGG 6 cut(s) 110, 820, 844, 988, 989, 1165
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 3 cut(s) 564, 690, 891
BseRI GAGGAG 3 cut(s) 665, 906, 986
BseXI GCAGC 2 cut(s) 163, 997
Bsh1236I CGCG 1 cut(s) 217
Bsh1285I CGRYCG 2 cut(s) 589, 615
BshFI GGCC 3 cut(s) 775, 878, 1092
BshNI GGYRCC 1 cut(s) 1246
BsiEI CGRYCG 2 cut(s) 589, 615
BsiHKAI GWGCWC 1 cut(s) 1372
BsiHKCI CYCGRG 1 cut(s) 880
BsiSI CCGG 5 cut(s) 186, 431, 615, 1270, 1347
BslFI GGGAC 1 cut(s) 789
BslI CCNNNNNNNGG 6 cut(s) 110, 820, 844, 988, 989, 1165
BsmAI GTCTC 4 cut(s) 634, 683, 944, 960
BsmBI CGTCTC 1 cut(s) 944
BsmFI GGGAC 1 cut(s) 789
BsmI GAATGC 3 cut(s) 665, 1310, 1316
BsnI GGCC 3 cut(s) 775, 878, 1092
BsoBI CYCGRG 1 cut(s) 880
Bsp1286I GDGCHC 1 cut(s) 1372
Bsp143I GATC 4 cut(s) 277, 586, 760, 1263
BspACI CCGC 3 cut(s) 138, 215, 217
BspANI GGCC 3 cut(s) 775, 878, 1092
BspCNI CTCAG 3 cut(s) 565, 691, 892
BspFNI CGCG 1 cut(s) 217
BspLI GGNNCC 2 cut(s) 915, 1248
BspQI GCTCTTC 2 cut(s) 688, 1377
BspT107I GGYRCC 1 cut(s) 1246
BsrDI GCAATG 1 cut(s) 175
BssECI CCNNGG 4 cut(s) 215, 838, 879, 1363
BssMI GATC 4 cut(s) 277, 586, 760, 1263
BssT1I CCWWGG 1 cut(s) 1363
Bst4CI ACNGT 5 cut(s) 28, 403, 501, 1027, 1129
Bst6I CTCTTC 5 cut(s) 688, 751, 924, 1112, 1377
BstC8I GCNNGC 1 cut(s) 978
BstDEI CTNAG 4 cut(s) 573, 699, 900, 1037
BstDSI CCRYGG 2 cut(s) 215, 838
BstENI CCTNNNNNAGG 1 cut(s) 1163
BstF5I GGATG 3 cut(s) 597, 721, 1375
BstFNI CGCG 1 cut(s) 217
BstKTI GATC 4 cut(s) 280, 589, 763, 1266
BstMAI GTCTC 4 cut(s) 634, 683, 944, 960
BstMBI GATC 4 cut(s) 277, 586, 760, 1263
BstMCI CGRYCG 2 cut(s) 589, 615
BstNSI RCATGY 1 cut(s) 1121
BstSCI CCNGG 1 cut(s) 185
BstSFI CTRYAG 2 cut(s) 180, 1409
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 163, 997
BstV2I GAAGAC 1 cut(s) 1320
BsuRI GGCC 3 cut(s) 775, 878, 1092
BtgI CCRYGG 2 cut(s) 215, 838
BtgZI GCGATG 1 cut(s) 500
BtsCI GGATG 3 cut(s) 597, 721, 1375
BtsIMutI CAGTG 1 cut(s) 1032
Cac8I GCNNGC 1 cut(s) 978
Cfr13I GGNCC 3 cut(s) 634, 774, 1090
Cfr42I CCGCGG 1 cut(s) 218
Csp6I GTAC 1 cut(s) 1247
CviAII CATG 5 cut(s) 446, 658, 672, 1118, 1171
CviQI GTAC 1 cut(s) 1247
DdeI CTNAG 4 cut(s) 573, 699, 900, 1037
DpnI GATC 4 cut(s) 279, 588, 762, 1265
DpnII GATC 4 cut(s) 277, 586, 760, 1263
DraI TTTAAA 1 cut(s) 438
Eam1104I CTCTTC 5 cut(s) 688, 751, 924, 1112, 1377
EarI CTCTTC 5 cut(s) 688, 751, 924, 1112, 1377
Ecl136II GAGCTC 1 cut(s) 1370
Eco130I CCWWGG 1 cut(s) 1363
Eco24I GRGCYC 1 cut(s) 1372
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 634
Eco53kI GAGCTC 1 cut(s) 1370
Eco57I CTGAAG 1 cut(s) 1148
Eco88I CYCGRG 1 cut(s) 880
EcoICRI GAGCTC 1 cut(s) 1370
EcoNI CCTNNNNNAGG 1 cut(s) 1163
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 1363
EcoT22I ATGCAT 1 cut(s) 1316
EcoT38I GRGCYC 1 cut(s) 1372
ErhI CCWWGG 1 cut(s) 1363
Esp3I CGTCTC 1 cut(s) 944
FaeI CATG 5 cut(s) 449, 661, 675, 1121, 1174
FalI AAGNNNNNCTT 2 cut(s) 942, 974
FaqI GGGAC 1 cut(s) 789
FatI CATG 5 cut(s) 445, 657, 671, 1117, 1170
Fnu4HI GCNGC 2 cut(s) 152, 1011
FokI GGATG 3 cut(s) 604, 728, 1362
FriOI GRGCYC 1 cut(s) 1372
Fsp4HI GCNGC 2 cut(s) 152, 1011
FspBI CTAG 3 cut(s) 105, 569, 1217
GluI GCNGC 2 cut(s) 152, 1011
GsuI CTGGAG 2 cut(s) 951, 985
HaeIII GGCC 3 cut(s) 775, 878, 1092
HapII CCGG 5 cut(s) 186, 431, 615, 1270, 1347
Hin1II CATG 5 cut(s) 449, 661, 675, 1121, 1174
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 7 cut(s) 86, 245, 343, 888, 953, 1101, 1254
HpaI GTTAAC 1 cut(s) 793
HpaII CCGG 5 cut(s) 186, 431, 615, 1270, 1347
HphI GGTGA 2 cut(s) 272, 546
Hpy166II GTNNAC 4 cut(s) 406, 637, 793, 1115
Hpy188I TCNGA 5 cut(s) 48, 85, 459, 591, 709
Hpy188III TCNNGA 7 cut(s) 329, 882, 923, 968, 1002, 1166, 1258
Hpy8I GTNNAC 4 cut(s) 406, 637, 793, 1115
HpyAV CCTTC 5 cut(s) 235, 293, 650, 680, 1169
HpyCH4III ACNGT 5 cut(s) 28, 403, 501, 1027, 1129
HpyCH4V TGCA 6 cut(s) 168, 522, 962, 1121, 1314, 1403
HpyF3I CTNAG 4 cut(s) 573, 699, 900, 1037
Hsp92II CATG 5 cut(s) 449, 661, 675, 1121, 1174
KpnI GGTACC 1 cut(s) 1250
KspAI GTTAAC 1 cut(s) 793
KspI CCGCGG 1 cut(s) 218
Kzo9I GATC 4 cut(s) 277, 586, 760, 1263
LguI GCTCTTC 2 cut(s) 688, 1377
LmnI GCTCC 5 cut(s) 106, 840, 919, 973, 1367
Lsp1109I GCAGC 2 cut(s) 163, 997
LweI GCATC 3 cut(s) 822, 1203, 1323
MaeI CTAG 3 cut(s) 105, 569, 1217
MaeIII GTNAC 4 cut(s) 113, 353, 1051, 1296
MalI GATC 4 cut(s) 279, 588, 762, 1265
MboI GATC 4 cut(s) 277, 586, 760, 1263
MhlI GDGCHC 1 cut(s) 1372
MluCI AATT 3 cut(s) 19, 529, 1333
MlyI GAGTC 3 cut(s) 337, 962, 1263
MmeI TCCRAC 2 cut(s) 716, 1410
Mph1103I ATGCAT 1 cut(s) 1316
MroXI GAANNNNTTC 1 cut(s) 930
MseI TTAA 7 cut(s) 60, 119, 144, 195, 437, 783, 792
MslI CAYNNNNRTG 2 cut(s) 670, 1175
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 5 cut(s) 186, 431, 615, 1270, 1347
MspR9I CCNGG 1 cut(s) 187
Mva1269I GAATGC 3 cut(s) 665, 1310, 1316
MvnI CGCG 1 cut(s) 217
NciI CCSGG 1 cut(s) 187
NdeII GATC 4 cut(s) 277, 586, 760, 1263
NlaIII CATG 5 cut(s) 449, 661, 675, 1121, 1174
NlaIV GGNNCC 2 cut(s) 915, 1248
NmeAIII GCCGAG 1 cut(s) 587
NmuCI GTSAC 1 cut(s) 1051
NsiI ATGCAT 1 cut(s) 1316
NspI RCATGY 1 cut(s) 1121
PciSI GCTCTTC 2 cut(s) 688, 1377
PcsI WCGNNNNNNNCGW 1 cut(s) 760
PctI GAATGC 3 cut(s) 665, 1310, 1316
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 4 cut(s) 86, 245, 888, 1101
PkrI GCNGC 2 cut(s) 153, 1012
Ple19I CGATCG 1 cut(s) 589
PleI GAGTC 3 cut(s) 337, 961, 1262
PpsI GAGTC 3 cut(s) 337, 961, 1262
PshBI ATTAAT 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 1372
PspN4I GGNNCC 2 cut(s) 915, 1248
PspPI GGNCC 3 cut(s) 634, 774, 1090
PvuI CGATCG 1 cut(s) 589
RsaI GTAC 1 cut(s) 1248
RsaNI GTAC 1 cut(s) 1247
RseI CAYNNNNRTG 2 cut(s) 670, 1175
SacI GAGCTC 1 cut(s) 1372
SacII CCGCGG 1 cut(s) 218
SapI GCTCTTC 2 cut(s) 688, 1377
SaqAI TTAA 7 cut(s) 60, 119, 144, 195, 437, 783, 792
SatI GCNGC 2 cut(s) 152, 1011
Sau3AI GATC 4 cut(s) 277, 586, 760, 1263
Sau96I GGNCC 3 cut(s) 634, 774, 1090
SchI GAGTC 3 cut(s) 337, 962, 1263
ScrFI CCNGG 1 cut(s) 187
SduI GDGCHC 1 cut(s) 1372
SfaNI GCATC 3 cut(s) 822, 1203, 1323
SfcI CTRYAG 2 cut(s) 180, 1409
Sfr303I CCGCGG 1 cut(s) 218
SgrBI CCGCGG 1 cut(s) 218
SinI GGWCC 1 cut(s) 634
SmiMI CAYNNNNRTG 2 cut(s) 670, 1175
SmlI CTYRAG 1 cut(s) 1059
SmoI CTYRAG 1 cut(s) 1059
Sse9I AATT 3 cut(s) 19, 529, 1333
SsiI CCGC 3 cut(s) 138, 215, 217
SspI AATATT 1 cut(s) 78
SspMI CTAG 3 cut(s) 105, 569, 1217
SstI GAGCTC 1 cut(s) 1372
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 1363
TaaI ACNGT 5 cut(s) 28, 403, 501, 1027, 1129
TaqI TCGA 5 cut(s) 585, 643, 866, 1096, 1280
TaqII GACCGA 1 cut(s) 622
TasI AATT 3 cut(s) 19, 529, 1333
TfiI GAWTC 4 cut(s) 86, 245, 888, 1101
Tru1I TTAA 7 cut(s) 60, 119, 144, 195, 437, 783, 792
Tru9I TTAA 7 cut(s) 60, 119, 144, 195, 437, 783, 792
TscAI CASTG 1 cut(s) 1032
TseFI GTSAC 1 cut(s) 1051
TseI GCWGC 2 cut(s) 151, 1010
Tsp45I GTSAC 1 cut(s) 1051
TspDTI ATGAA 5 cut(s) 21, 87, 274, 1220, 1364
TspGWI ACGGA 2 cut(s) 810, 827
TspRI CASTG 1 cut(s) 1032
VpaK11BI GGWCC 1 cut(s) 634
VspI ATTAAT 1 cut(s) 60
XagI CCTNNNNNAGG 1 cut(s) 1163
XceI RCATGY 1 cut(s) 1121
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 3 cut(s) 105, 569, 1217
Zsp2I ATGCAT 1 cut(s) 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.