MD15G1356100.v1.1

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008279
Physical Location & Seq
Forward (+)
42948605 .. 42952728
4124 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1356100.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGGGAATGAAAGCAAAGGCCCACCACCACCATTACAATGCTAATGGTGGTGGTGGTGGAGGCAACGGCAGTGAGAGCAGCAGCAGCACCTCACCTAGCCCCCCTCCGTCGCCGCGCCGTCACTCTACAGTCCCTCACTGCCGCCGGAAGCTCCGGACAAAACCCATCTCCATCGTCTTCAGTTTTCTTCTCCTCCGCCGATTCCTCCGCTTACTGGTGGTTCTTCCCCTGCTATACGTCTCTGGCCTGCTCATGTGTGTGGGCCCGTTCTCTGGTCTAGTGGGTCATACCCCTCTTCCCGGTTCCGTTTACCGCAGTCACGAGATGTTTCAGCTGCTCTGGCCTCACATTCAGGCCGATAACTCGACTCCGATTGAGTTGTCCTCTGTATGGAGATTCAGAAGGAAGTTGAAAGAGCAGAAGCCTTGCCCACCTTCAGCTTCTGGACTACAATTAGATTCCCCAGGCCTTACTGGTTACTTAATTGTGGAGGTTAATGGTGGCCTTAACCAACAACGCTCTGCGATTTGCAATGCTGTGGCCCTTGCTGGTCTTTTGAATGCAGTTCTGGTTATTCCTCGCTTTGAATTCCATAACGTTTGGAGGGATACAAGCACATTTACTGATATATACGATGAAGATCATTTCATAGCCACCCTTGAAGGCCATGTTAAAGTGGTTAAAGAACTACCGGAGATGCTGATGGATAAATTTGATCACAACATCTCTAACATACCAACCCTTCGTGTCCAAGCTTGGGCCCCTGTCCGTTATTACACAGGAGAAGTTTATCCTGTTCTGCAGAAGCAGGGGGTTATCCGCATAGCTCCATTTGCTAATAGATTAGCAATGAATGTCCCACCTCCTATTCAGCATTTGAGATGCCTCACTAATTATGAAGCATTGAAATTCTCTTCTCCCATATCAAATTTGGCAACTAAACTAGTCGAACGAATGACTGAAATGAGTTCAAGGACCAGTGGAAAGTATGTTGCAGTCCACCTCCGTTTTGAGGAGGACATGGTGGCATTCTCATGCTGTTTGTATGATGGAGGAGAGGCTGAAAATGTCGCAATGAATTCAATTCGACAAAAAGGATGGGGAGATAAGTTCAAGAGAAAAGGCCGTGTAACTCTACCTGGTCTCAACAGGATTGATGGAAAATGCCCACTTACCCCCTTGGAGGTTGGAATGATGTTACGGGGTATGGGATTTGATAACAGCACTTCAATTTATTTAGCCTCTGGGAAAATCTACTGGGCGGAGAAACATTTAGCCCCTCTGCTCAAGTTGTTTCCCTATCTGCATACCAAGGAGTCTCTTGCAACCCCAGATGAGCTTGCTCCTTTTGAGGGTTATTCTTCAAGATTGGCAGCTTTGGATTATACAGTATGCTTGTTTAGTGAGTTTTTTGTAACAACACAAGGTGGGAACTTCCCACATTTTCTAATGGGTCATCGGAGATTCTCCTATGGTGGGCATGCCAAGACAATTATACCTGATAAGCGCAAGCTTGTTGTTCTACTGCAGAACATGAGTATCAGTTGGAAAGATTTCAAACTTGAGATGGAAGCAATGCTCACAGAAAGTGACCGCAAGGGAAAGATAATACCGAGAGTGAAAAAATTCACCCGAAAATCTTCTGTATACACGTACCCTTTGCCAGAGTGTAGTTGTCTCCAAAAGCACAACAACACTCAGATTGTACCATCTGCACATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

575

Amino Acids

64.41

Weight (kDa)

9.53

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 160 - 489 9.1e-97 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1647
AccII CGCG 1 cut(s) 115
AccIII TCCGGA 1 cut(s) 153
AciI CCGC 8 cut(s) 113, 142, 196, 208, 313, 820, 1262, 1594
AclI AACGTT 1 cut(s) 597
AcsI RAATTY 6 cut(s) 587, 710, 908, 928, 1078, 1625
AcuI CTGAAG 2 cut(s) 163, 420
AdeI CACNNNGTG 1 cut(s) 1427
AfaI GTAC 2 cut(s) 1655, 1707
AfiI CCNNNNNNNGG 9 cut(s) 214, 272, 299, 390, 757, 1012, 1183, 1352, 1476
AflIII ACRYGT 1 cut(s) 1650
AhlI ACTAGT 1 cut(s) 943
AjnI CCWGG 2 cut(s) 463, 1138
AluBI AGCT 8 cut(s) 151, 334, 440, 755, 827, 1339, 1376, 1513
AluI AGCT 8 cut(s) 151, 334, 440, 755, 827, 1339, 1376, 1513
Alw26I GTCTC 4 cut(s) 244, 1148, 1323, 1682
AlwNI CAGNNNCTG 1 cut(s) 443
Aor13HI TCCGGA 1 cut(s) 153
ApaI GGGCCC 2 cut(s) 266, 763
ApeKI GCWGC 5 cut(s) 78, 81, 84, 334, 1373
ApoI RAATTY 6 cut(s) 587, 710, 908, 928, 1078, 1625
Asp700I GAANNNNTTC 3 cut(s) 1553, 1625, 1639
AspLEI GCGC 2 cut(s) 117, 1509
AspS9I GGNCC 7 cut(s) 19, 262, 263, 541, 759, 760, 975
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 2 cut(s) 84, 1621
AvaII GGWCC 1 cut(s) 975
BaeGI GKGCMC 2 cut(s) 266, 763
BaeI ACNNNNGTAYC 2 cut(s) 1522, 1555
BanII GRGCYC 2 cut(s) 266, 763
BauI CACGAG 1 cut(s) 320
BbsI GAAGAC 1 cut(s) 169
BbvI GCAGC 5 cut(s) 90, 93, 96, 321, 1385
BccI CCATC 8 cut(s) 173, 179, 697, 1043, 1092, 1151, 1561, 1717
BceAI ACGGC 3 cut(s) 82, 102, 1110
BciT130I CCWGG 2 cut(s) 465, 1140
BciVI GTATCC 1 cut(s) 601
BclI TGATCA 1 cut(s) 715
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 4 cut(s) 244, 1148, 1323, 1682
BcuI ACTAGT 1 cut(s) 943
BfaI CTAG 3 cut(s) 96, 278, 944
BfmI CTRYAG 3 cut(s) 126, 800, 1526
BfuI GTATCC 1 cut(s) 601
BisI GCNGC 7 cut(s) 79, 82, 85, 113, 142, 335, 1374
BlsI GCNGC 7 cut(s) 80, 83, 86, 114, 143, 336, 1375
Bme1390I CCNGG 3 cut(s) 300, 465, 1140
Bme18I GGWCC 1 cut(s) 975
BmgT120I GGNCC 7 cut(s) 19, 262, 263, 541, 759, 760, 975
BmiI GGNNCC 4 cut(s) 264, 304, 761, 762
BmrFI CCNGG 3 cut(s) 300, 465, 1140
BmrI ACTGGG 1 cut(s) 1267
BmsI GCATC 2 cut(s) 687, 872
BmuI ACTGGG 1 cut(s) 1267
BpiI GAAGAC 1 cut(s) 169
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpuEI CTTGAG 2 cut(s) 1271, 1583
BpuMI CCSGG 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 1653
BsaBI GATNNNNATC 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 1148
BsaJI CCNNGG 3 cut(s) 463, 1179, 1311
BsaWI WCCGGW 2 cut(s) 153, 691
Bsc4I CCNNNNNNNGG 9 cut(s) 214, 272, 299, 390, 757, 1012, 1183, 1352, 1476
Bse1I ACTGG 4 cut(s) 219, 478, 978, 1262
Bse3DI GCAATG 4 cut(s) 538, 855, 1080, 1581
Bse8I GATNNNNATC 1 cut(s) 639
BseAI TCCGGA 1 cut(s) 153
BseBI CCWGG 2 cut(s) 465, 1140
BseDI CCNNGG 3 cut(s) 463, 1179, 1311
BseGI GGATG 1 cut(s) 1103
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 9 cut(s) 214, 272, 299, 390, 757, 1012, 1183, 1352, 1476
BseMI GCAATG 4 cut(s) 538, 855, 1080, 1581
BseMII CTCAG 1 cut(s) 1712
BseNI ACTGG 4 cut(s) 219, 478, 978, 1262
BseRI GAGGAG 3 cut(s) 182, 1028, 1068
BseSI GKGCMC 2 cut(s) 266, 763
BseXI GCAGC 5 cut(s) 90, 93, 96, 321, 1385
BsgI GTGCAG 1 cut(s) 1698
Bsh1236I CGCG 1 cut(s) 115
BsiSI CCGG 4 cut(s) 145, 154, 300, 692
BslFI GGGAC 2 cut(s) 116, 842
BslI CCNNNNNNNGG 9 cut(s) 214, 272, 299, 390, 757, 1012, 1183, 1352, 1476
BsmAI GTCTC 4 cut(s) 244, 1148, 1323, 1682
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 2 cut(s) 116, 842
BsmI GAATGC 2 cut(s) 565, 1028
Bso31I GGTCTC 1 cut(s) 1148
Bsp120I GGGCCC 2 cut(s) 262, 759
Bsp1286I GDGCHC 2 cut(s) 266, 763
Bsp13I TCCGGA 1 cut(s) 153
Bsp143I GATC 2 cut(s) 640, 715
BspACI CCGC 8 cut(s) 113, 142, 196, 208, 313, 820, 1262, 1594
BspCNI CTCAG 1 cut(s) 1711
BspEI TCCGGA 1 cut(s) 153
BspFNI CGCG 1 cut(s) 115
BspLI GGNNCC 4 cut(s) 264, 304, 761, 762
BspMAI CTGCAG 2 cut(s) 804, 1530
BspTNI GGTCTC 1 cut(s) 1148
BsrDI GCAATG 4 cut(s) 538, 855, 1080, 1581
BsrI ACTGG 4 cut(s) 219, 478, 978, 1262
BssECI CCNNGG 3 cut(s) 463, 1179, 1311
BssMI GATC 2 cut(s) 640, 715
BssNAI GTATAC 1 cut(s) 1648
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 2 cut(s) 1179, 1311
Bst1107I GTATAC 1 cut(s) 1648
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 2 cut(s) 465, 1140
Bst4CI ACNGT 2 cut(s) 130, 1390
Bst6I CTCTTC 2 cut(s) 300, 919
BstBAI YACGTR 1 cut(s) 1653
BstC8I GCNNGC 4 cut(s) 248, 1341, 1482, 1511
BstDEI CTNAG 1 cut(s) 1698
BstF5I GGATG 1 cut(s) 1103
BstFNI CGCG 1 cut(s) 115
BstHHI GCGC 2 cut(s) 117, 1509
BstKTI GATC 2 cut(s) 643, 718
BstMAI GTCTC 4 cut(s) 244, 1148, 1323, 1682
BstMBI GATC 2 cut(s) 640, 715
BstMWI GCNNNNNNNGC 4 cut(s) 75, 84, 340, 833
BstNI CCWGG 2 cut(s) 465, 1140
BstNSI RCATGY 1 cut(s) 1484
BstSCI CCNGG 3 cut(s) 298, 463, 1138
BstSFI CTRYAG 3 cut(s) 126, 800, 1526
BstSLI GKGCMC 2 cut(s) 266, 763
BstUI CGCG 1 cut(s) 115
BstV1I GCAGC 5 cut(s) 90, 93, 96, 321, 1385
BstV2I GAAGAC 1 cut(s) 169
BstZ17I GTATAC 1 cut(s) 1648
BsuI GTATCC 1 cut(s) 601
BtsCI GGATG 1 cut(s) 1103
BtsI GCAGTG 2 cut(s) 76, 136
BtsIMutI CAGTG 3 cut(s) 76, 136, 985
Cac8I GCNNGC 4 cut(s) 248, 1341, 1482, 1511
CaiI CAGNNNCTG 1 cut(s) 443
CfoI GCGC 2 cut(s) 117, 1509
Cfr13I GGNCC 7 cut(s) 19, 262, 263, 541, 759, 760, 975
CsiI ACCWGGT 1 cut(s) 1138
Csp6I GTAC 2 cut(s) 1654, 1706
CviAII CATG 6 cut(s) 253, 668, 1021, 1035, 1481, 1534
CviQI GTAC 2 cut(s) 1654, 1706
DdeI CTNAG 1 cut(s) 1698
DpnI GATC 2 cut(s) 642, 717
DpnII GATC 2 cut(s) 640, 715
DraIII CACNNNGTG 1 cut(s) 1427
Eam1104I CTCTTC 2 cut(s) 300, 919
EarI CTCTTC 2 cut(s) 300, 919
EciI GGCGGA 2 cut(s) 185, 1277
Eco130I CCWWGG 2 cut(s) 1179, 1311
Eco147I AGGCCT 1 cut(s) 468
Eco24I GRGCYC 2 cut(s) 266, 763
Eco31I GGTCTC 1 cut(s) 1148
Eco47I GGWCC 1 cut(s) 975
Eco57I CTGAAG 2 cut(s) 163, 420
EcoO109I RGGNCCY 1 cut(s) 760
EcoRI GAATTC 2 cut(s) 587, 1078
EcoRII CCWGG 2 cut(s) 463, 1138
EcoT14I CCWWGG 2 cut(s) 1179, 1311
EcoT38I GRGCYC 2 cut(s) 266, 763
ErhI CCWWGG 2 cut(s) 1179, 1311
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 6 cut(s) 256, 671, 1024, 1038, 1484, 1537
FaqI GGGAC 2 cut(s) 116, 842
FatI CATG 6 cut(s) 252, 667, 1020, 1034, 1480, 1533
FbaI TGATCA 1 cut(s) 715
FblI GTMKAC 1 cut(s) 1647
Fnu4HI GCNGC 7 cut(s) 79, 82, 85, 113, 142, 335, 1374
FokI GGATG 1 cut(s) 1110
FriOI GRGCYC 2 cut(s) 266, 763
Fsp4HI GCNGC 7 cut(s) 79, 82, 85, 113, 142, 335, 1374
FspBI CTAG 3 cut(s) 96, 278, 944
GlaI GCGC 2 cut(s) 116, 1508
GluI GCNGC 7 cut(s) 79, 82, 85, 113, 142, 335, 1374
HapII CCGG 4 cut(s) 145, 154, 300, 692
HhaI GCGC 2 cut(s) 117, 1509
Hin1II CATG 6 cut(s) 256, 671, 1024, 1038, 1484, 1537
Hin6I GCGC 2 cut(s) 115, 1507
HinP1I GCGC 2 cut(s) 115, 1507
HindIII AAGCTT 2 cut(s) 753, 1511
HinfI GANTC 6 cut(s) 201, 367, 396, 458, 1316, 1464
HpaII CCGG 4 cut(s) 145, 154, 300, 692
HphI GGTGA 2 cut(s) 84, 1621
Hpy166II GTNNAC 3 cut(s) 310, 1000, 1648
Hpy188I TCNGA 4 cut(s) 372, 401, 1461, 1701
Hpy188III TCNNGA 6 cut(s) 154, 320, 444, 1114, 1365, 1722
Hpy8I GTNNAC 3 cut(s) 310, 1000, 1648
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 4 cut(s) 396, 444, 656, 752
HpyCH4III ACNGT 2 cut(s) 130, 1390
HpyCH4IV ACGT 3 cut(s) 237, 597, 1652
HpyCH4V TGCA 8 cut(s) 531, 563, 802, 995, 1306, 1325, 1528, 1715
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 84, 340, 833
HpyF3I CTNAG 1 cut(s) 1698
HpySE526I ACGT 3 cut(s) 237, 597, 1652
Hsp92II CATG 6 cut(s) 256, 671, 1024, 1038, 1484, 1537
HspAI GCGC 2 cut(s) 115, 1507
Kpn2I TCCGGA 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 2 cut(s) 640, 715
LmnI GCTCC 3 cut(s) 156, 832, 1348
Lsp1109I GCAGC 5 cut(s) 90, 93, 96, 321, 1385
LweI GCATC 2 cut(s) 687, 872
MabI ACCWGGT 1 cut(s) 1138
MaeI CTAG 3 cut(s) 96, 278, 944
MaeII ACGT 3 cut(s) 237, 597, 1652
MaeIII GTNAC 7 cut(s) 119, 317, 476, 1129, 1197, 1414, 1589
MalI GATC 2 cut(s) 642, 717
MboI GATC 2 cut(s) 640, 715
MboII GAAGA 8 cut(s) 169, 179, 215, 287, 650, 906, 1353, 1632
MhlI GDGCHC 2 cut(s) 266, 763
MlyI GAGTC 2 cut(s) 361, 1325
MmeI TCCRAC 2 cut(s) 1168, 1526
MroI TCCGGA 1 cut(s) 153
MroXI GAANNNNTTC 3 cut(s) 1553, 1625, 1639
MseI TTAA 5 cut(s) 482, 495, 507, 672, 681
MslI CAYNNNNRTG 3 cut(s) 36, 257, 1033
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 4 cut(s) 145, 154, 300, 692
MspR9I CCNGG 3 cut(s) 300, 465, 1140
Mva1269I GAATGC 2 cut(s) 565, 1028
MvaI CCWGG 2 cut(s) 465, 1140
MvnI CGCG 1 cut(s) 115
MwoI GCNNNNNNNGC 4 cut(s) 75, 84, 340, 833
NciI CCSGG 1 cut(s) 300
NdeII GATC 2 cut(s) 640, 715
NlaIII CATG 6 cut(s) 256, 671, 1024, 1038, 1484, 1537
NlaIV GGNNCC 4 cut(s) 264, 304, 761, 762
NmuCI GTSAC 3 cut(s) 119, 317, 1589
NspI RCATGY 1 cut(s) 1484
PaeI GCATGC 1 cut(s) 1484
PceI AGGCCT 1 cut(s) 468
PctI GAATGC 2 cut(s) 565, 1028
PdmI GAANNNNTTC 3 cut(s) 1553, 1625, 1639
PfeI GAWTC 4 cut(s) 201, 396, 458, 1464
PkrI GCNGC 7 cut(s) 80, 83, 86, 114, 143, 336, 1375
PleI GAGTC 2 cut(s) 361, 1324
PpsI GAGTC 2 cut(s) 361, 1324
Ppu21I YACGTR 1 cut(s) 1653
Psp1406I AACGTT 1 cut(s) 597
Psp6I CCWGG 2 cut(s) 463, 1138
PspGI CCWGG 2 cut(s) 463, 1138
PspN4I GGNNCC 4 cut(s) 264, 304, 761, 762
PspOMI GGGCCC 2 cut(s) 262, 759
PspPI GGNCC 7 cut(s) 19, 262, 263, 541, 759, 760, 975
PstI CTGCAG 2 cut(s) 804, 1530
PstNI CAGNNNCTG 1 cut(s) 443
PvuII CAGCTG 1 cut(s) 334
RsaI GTAC 2 cut(s) 1655, 1707
RsaNI GTAC 2 cut(s) 1654, 1706
RseI CAYNNNNRTG 3 cut(s) 36, 257, 1033
SaqAI TTAA 5 cut(s) 482, 495, 507, 672, 681
SatI GCNGC 7 cut(s) 79, 82, 85, 113, 142, 335, 1374
Sau3AI GATC 2 cut(s) 640, 715
Sau96I GGNCC 7 cut(s) 19, 262, 263, 541, 759, 760, 975
SchI GAGTC 2 cut(s) 361, 1325
ScrFI CCNGG 3 cut(s) 300, 465, 1140
SduI GDGCHC 2 cut(s) 266, 763
SexAI ACCWGGT 1 cut(s) 1138
SfaNI GCATC 2 cut(s) 687, 872
SfcI CTRYAG 3 cut(s) 126, 800, 1526
SinI GGWCC 1 cut(s) 975
SmiMI CAYNNNNRTG 3 cut(s) 36, 257, 1033
SmlI CTYRAG 2 cut(s) 1286, 1562
SmoI CTYRAG 2 cut(s) 1286, 1562
SpeI ACTAGT 1 cut(s) 943
SphI GCATGC 1 cut(s) 1484
SseBI AGGCCT 1 cut(s) 468
SsiI CCGC 8 cut(s) 113, 142, 196, 208, 313, 820, 1262, 1594
SspMI CTAG 3 cut(s) 96, 278, 944
StuI AGGCCT 1 cut(s) 468
StyD4I CCNGG 3 cut(s) 298, 463, 1138
StyI CCWWGG 2 cut(s) 1179, 1311
TaaI ACNGT 2 cut(s) 130, 1390
TaiI ACGT 3 cut(s) 240, 600, 1655
TaqI TCGA 3 cut(s) 365, 948, 1087
TauI GCSGC 2 cut(s) 115, 144
TfiI GAWTC 4 cut(s) 201, 396, 458, 1464
Tru1I TTAA 5 cut(s) 482, 495, 507, 672, 681
Tru9I TTAA 5 cut(s) 482, 495, 507, 672, 681
TscAI CASTG 3 cut(s) 76, 143, 985
TseFI GTSAC 3 cut(s) 119, 317, 1589
TseI GCWGC 5 cut(s) 78, 81, 84, 334, 1373
Tsp45I GTSAC 3 cut(s) 119, 317, 1589
TspDTI ATGAA 6 cut(s) 23, 637, 651, 866, 912, 1091
TspGWI ACGGA 4 cut(s) 96, 295, 758, 995
TspRI CASTG 3 cut(s) 76, 143, 985
VpaK11BI GGWCC 1 cut(s) 975
XapI RAATTY 6 cut(s) 587, 710, 908, 928, 1078, 1625
XceI RCATGY 1 cut(s) 1484
XcmI CCANNNNNNNNNTGG 1 cut(s) 928
XmiI GTMKAC 1 cut(s) 1647
XmnI GAANNNNTTC 3 cut(s) 1553, 1625, 1639
XspI CTAG 3 cut(s) 96, 278, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.