AT1G38131

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008279
Physical Location & Seq
Reverse (-)
14292996 .. 14296253
3258 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G38131.1

Sequence Viewer

Length: 1770 bp
ATGAAGAGCAAAATCCACCACCAGCCCAATGGTAGCAACAATGGTGTTGTTTCAAGTAACGATAATGGCTGCCGAAGTGAGTCTCCAAGCCCACCTTTATCTCCAAACCGCCGTGTCCTCCGTCGTCAACGGCGGCAAACTTTACTCAGAGCTTCTTCTTTCTCTCTCCGGAGAAATCTTCGTTACTTGCTTCTCCTTCCTATGATTTACGCTTCTGGGCTTCTCATGTGTGTTGGTCCTTTCTCTGGTCTTGTCGGATGGGTTTATGTTCCCGGTTCTGTTTATCGGAGCCCCGAGATTTACCGGAAACTTAAAGATGACATTTTCTCCGACAATTCAACCGCCCTTGAGTTGTCTTCTGTCTGGAAATTCAAAAGAAGACCAAAGATGCCAAAACCTTGTCCTAATTCAACAGTTTCATCACATTTTGGTCTGAACAGAGAATCATCTGCTCTTGCTCCTAGTAGTGGTTACTTAATCGTGGAAGCAAATGGCGGTCTTAATCAGCAGCGTTCTGCGATTTGCAATGCTGTGGCTGTAGCCGGGCTTCTTAACGCAGTTTTAGTCATACCGCGGTTTGAGTTTCACGCTATTTGGAAGGATTCAAGCAACTTTGGTGATATCTATGACGAAGATCACTTCATATCAAGCCTTGAAGGTTATGTGAAAATAGTGAGAGATGTTCCTGATGAGATAATGACTCGTTTTAGTTACAATGTTTCAAGTATTCCTACTATTCGTGTTCAAGCTTGGGCTACTGTGAACTACTATAACGGGGAAGTTTATCCGGTTTTAAAGGAACATGGGGTTATTCGGATAACTCCTTTCGCAAATAGATTGGCGATGAGTGTTCCACCGTATATACAACTTTTGAGGTGCATAGCTAATTACAAAGCATTGAAGTTTTCTTCACCTATATCAACACTAGCTGAGAAGTTAGTCGATCGGATGGTTGAGAAAAGCTCGGCGACCGGAGGAAAGTATGTCTCGGTCCACCTTCGATTTGAGGAGGACATGGTGGCATTCTCATGTTGTCTCTACGAAGGTGGAAGAGCTGAGAAATCTGAAATGGATGTGATTAGACAGAAAAGTTGGAAAGGAAAGTTCAAACGAAGAGATCGTGTCATTAGGCCCGACCTTAACCGAGTTAACGGAAAATGTCCCCTTACCCCATTAGAGGTCGGTATGATGCTCCGTGGTATGGGATTTGATAACAACACTTCGATATATTTGGCCTCGGGAAGAATCTACCAACCTGAGAAACATTTGGCTCCTCTTCAAGAAATGTTTCCTCGTCTCTACACAAAAGAGTCTCTTGCAACTCCAGAGGAGCTTGCCCCATTTCAGGGATATTCCTCCAGAATGGCTGCTTTGGACTACACAGTGAGTTTGCTAAGTGAAGTCTTTGTGACAACTCAAGGTGGAAACTTTCCTCATTTTTTGATGGGCCATCGAAGATTCCTCTTCGGTGGACATGCAAAAACTGTCATACCCGACAAACCCAAACTCGTTCTCCTTCTTCAGGACATGGAAATGAGGTGGGAAGTTTTCAAAAAGGAGATGAAACTGATGCTAGGGGAAAGCGATAGAAAGGGAGTGATGGTACCGAGAGTCAGGAAGATCAACCGGAAAACTTCGATTTACACATACCCGTTACCCGAATGCGAATGCATCTTCCATTTGTCTTCCAATTTTTCAAACACCGGAAACATTTTGAGCCTTGGAGCTCTTCATCCATCTTCCAACCTCATAAGTTCTGCAAGACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

589

Amino Acids

66.49

Weight (kDa)

9.66

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 158 - 487 1.7e-100 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1603
AccB1I GGYRCC 1 cut(s) 1603
AccII CGCG 1 cut(s) 574
AccIII TCCGGA 1 cut(s) 168
AciI CCGC 6 cut(s) 109, 133, 342, 495, 572, 574
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 1505
AfaI GTAC 1 cut(s) 1605
AfiI CCNNNNNNNGG 7 cut(s) 245, 467, 1177, 1201, 1345, 1346, 1522
AluBI AGCT 8 cut(s) 152, 749, 884, 929, 963, 1055, 1333, 1727
AluI AGCT 8 cut(s) 152, 749, 884, 929, 963, 1055, 1333, 1727
Alw21I GWGCWC 1 cut(s) 1729
Alw26I GTCTC 5 cut(s) 87, 991, 1040, 1301, 1317
Ama87I CYCGRG 2 cut(s) 293, 1237
Aor13HI TCCGGA 1 cut(s) 168
AoxI GGCC 3 cut(s) 1130, 1233, 1447
ApeKI GCWGC 3 cut(s) 69, 508, 1367
ApoI RAATTY 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 1287
Asp718I GGTACC 1 cut(s) 1603
AspS9I GGNCC 4 cut(s) 236, 991, 1131, 1447
AsuC2I CCSGG 2 cut(s) 273, 544
AsuHPI GGTGA 2 cut(s) 629, 903
AvaI CYCGRG 2 cut(s) 293, 1237
AvaII GGWCC 2 cut(s) 236, 991
BanI GGYRCC 1 cut(s) 1603
BanII GRGCYC 2 cut(s) 293, 1729
BarI GAAGNNNNNNTAC 2 cut(s) 531, 563
BbsI GAAGAC 3 cut(s) 348, 385, 1677
Bbv12I GWGCWC 1 cut(s) 1729
BbvI GCAGC 3 cut(s) 56, 520, 1354
BccI CCATC 6 cut(s) 252, 943, 1438, 1458, 1594, 1744
BceAI ACGGC 2 cut(s) 96, 146
BcnI CCSGG 2 cut(s) 273, 544
BcoDI GTCTC 5 cut(s) 87, 991, 1040, 1301, 1317
BfaI CTAG 3 cut(s) 462, 926, 1574
BfmI CTRYAG 2 cut(s) 537, 1766
BisI GCNGC 4 cut(s) 70, 134, 509, 1368
BlsI GCNGC 4 cut(s) 71, 135, 510, 1369
Bme1390I CCNGG 2 cut(s) 273, 544
Bme18I GGWCC 2 cut(s) 236, 991
BmeT110I CYCGRG 2 cut(s) 293, 1237
BmgT120I GGNCC 4 cut(s) 236, 991, 1131, 1447
BmiI GGNNCC 3 cut(s) 290, 1272, 1605
BmrFI CCNGG 2 cut(s) 273, 544
BmsI GCATC 4 cut(s) 378, 1179, 1560, 1680
BpiI GAAGAC 3 cut(s) 348, 385, 1677
BplI GAGNNNNNCTC 2 cut(s) 947, 979
BpmI CTGGAG 2 cut(s) 1308, 1342
BpuEI CTTGAG 2 cut(s) 368, 1401
BpuMI CCSGG 2 cut(s) 273, 544
BsaJI CCNNGG 4 cut(s) 572, 1195, 1236, 1720
BsaWI WCCGGW 6 cut(s) 168, 303, 787, 971, 1626, 1703
BsaXI ACNNNNNCTCC 6 cut(s) 311, 341, 1497, 1527, 1587, 1617
Bsc4I CCNNNNNNNGG 7 cut(s) 245, 467, 1177, 1201, 1345, 1346, 1522
Bse3DI GCAATG 1 cut(s) 532
BseAI TCCGGA 1 cut(s) 168
BseDI CCNNGG 4 cut(s) 572, 1195, 1236, 1720
BseGI GGATG 4 cut(s) 263, 954, 1078, 1732
BseLI CCNNNNNNNGG 7 cut(s) 245, 467, 1177, 1201, 1345, 1346, 1522
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 4 cut(s) 160, 921, 1047, 1248
BseRI GAGGAG 3 cut(s) 1022, 1263, 1343
BseXI GCAGC 3 cut(s) 56, 520, 1354
Bsh1236I CGCG 1 cut(s) 574
Bsh1285I CGRYCG 2 cut(s) 946, 972
BshFI GGCC 3 cut(s) 1132, 1235, 1449
BshNI GGYRCC 1 cut(s) 1603
BsiEI CGRYCG 2 cut(s) 946, 972
BsiHKAI GWGCWC 1 cut(s) 1729
BsiHKCI CYCGRG 2 cut(s) 293, 1237
BsiSI CCGG 8 cut(s) 169, 273, 304, 543, 788, 972, 1627, 1704
BslFI GGGAC 1 cut(s) 1146
BslI CCNNNNNNNGG 7 cut(s) 245, 467, 1177, 1201, 1345, 1346, 1522
BsmAI GTCTC 5 cut(s) 87, 991, 1040, 1301, 1317
BsmBI CGTCTC 1 cut(s) 1301
BsmFI GGGAC 1 cut(s) 1146
BsmI GAATGC 3 cut(s) 1022, 1667, 1673
BsnI GGCC 3 cut(s) 1132, 1235, 1449
BsoBI CYCGRG 2 cut(s) 293, 1237
Bsp1286I GDGCHC 2 cut(s) 293, 1729
Bsp13I TCCGGA 1 cut(s) 168
Bsp143I GATC 4 cut(s) 634, 943, 1117, 1620
BspACI CCGC 6 cut(s) 109, 133, 342, 495, 572, 574
BspANI GGCC 3 cut(s) 1132, 1235, 1449
BspCNI CTCAG 4 cut(s) 159, 922, 1048, 1249
BspEI TCCGGA 1 cut(s) 168
BspFNI CGCG 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 290, 1272, 1605
BspQI GCTCTTC 2 cut(s) 1045, 1734
BspT107I GGYRCC 1 cut(s) 1603
BsrDI GCAATG 1 cut(s) 532
BssECI CCNNGG 4 cut(s) 572, 1195, 1236, 1720
BssMI GATC 4 cut(s) 634, 943, 1117, 1620
BssT1I CCWWGG 1 cut(s) 1720
Bst4CI ACNGT 5 cut(s) 415, 760, 858, 1384, 1486
Bst6I CTCTTC 5 cut(s) 1045, 1108, 1281, 1469, 1734
BstC8I GCNNGC 1 cut(s) 1335
BstDEI CTNAG 5 cut(s) 146, 930, 1056, 1257, 1394
BstDSI CCRYGG 2 cut(s) 572, 1195
BstENI CCTNNNNNAGG 1 cut(s) 1520
BstF5I GGATG 4 cut(s) 263, 954, 1078, 1732
BstFNI CGCG 1 cut(s) 574
BstKTI GATC 4 cut(s) 637, 946, 1120, 1623
BstMAI GTCTC 5 cut(s) 87, 991, 1040, 1301, 1317
BstMBI GATC 4 cut(s) 634, 943, 1117, 1620
BstMCI CGRYCG 2 cut(s) 946, 972
BstNSI RCATGY 1 cut(s) 1478
BstSCI CCNGG 2 cut(s) 271, 542
BstSFI CTRYAG 2 cut(s) 537, 1766
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 3 cut(s) 56, 520, 1354
BstV2I GAAGAC 3 cut(s) 348, 385, 1677
BstXI CCANNNNNNTGG 1 cut(s) 29
BsuRI GGCC 3 cut(s) 1132, 1235, 1449
BtgI CCRYGG 2 cut(s) 572, 1195
BtgZI GCGATG 1 cut(s) 857
BtsCI GGATG 4 cut(s) 263, 954, 1078, 1732
BtsIMutI CAGTG 1 cut(s) 1389
Cac8I GCNNGC 1 cut(s) 1335
Cfr13I GGNCC 4 cut(s) 236, 991, 1131, 1447
Cfr42I CCGCGG 1 cut(s) 575
Csp6I GTAC 1 cut(s) 1604
CviAII CATG 6 cut(s) 226, 803, 1015, 1029, 1475, 1528
CviQI GTAC 1 cut(s) 1604
DdeI CTNAG 5 cut(s) 146, 930, 1056, 1257, 1394
DpnI GATC 4 cut(s) 636, 945, 1119, 1622
DpnII GATC 4 cut(s) 634, 943, 1117, 1620
DraI TTTAAA 1 cut(s) 795
Eam1104I CTCTTC 5 cut(s) 1045, 1108, 1281, 1469, 1734
EarI CTCTTC 5 cut(s) 1045, 1108, 1281, 1469, 1734
Ecl136II GAGCTC 1 cut(s) 1727
Eco130I CCWWGG 1 cut(s) 1720
Eco24I GRGCYC 2 cut(s) 293, 1729
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 2 cut(s) 236, 991
Eco53kI GAGCTC 1 cut(s) 1727
Eco57I CTGAAG 1 cut(s) 1505
Eco88I CYCGRG 2 cut(s) 293, 1237
EcoICRI GAGCTC 1 cut(s) 1727
EcoNI CCTNNNNNAGG 1 cut(s) 1520
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 1720
EcoT22I ATGCAT 1 cut(s) 1673
EcoT38I GRGCYC 2 cut(s) 293, 1729
ErhI CCWWGG 1 cut(s) 1720
Esp3I CGTCTC 1 cut(s) 1301
FaeI CATG 6 cut(s) 229, 806, 1018, 1032, 1478, 1531
FalI AAGNNNNNCTT 4 cut(s) 79, 111, 1299, 1331
FaqI GGGAC 1 cut(s) 1146
FatI CATG 6 cut(s) 225, 802, 1014, 1028, 1474, 1527
Fnu4HI GCNGC 4 cut(s) 70, 134, 509, 1368
FokI GGATG 4 cut(s) 270, 961, 1085, 1719
FriOI GRGCYC 2 cut(s) 293, 1729
Fsp4HI GCNGC 4 cut(s) 70, 134, 509, 1368
FspBI CTAG 3 cut(s) 462, 926, 1574
GluI GCNGC 4 cut(s) 70, 134, 509, 1368
GsuI CTGGAG 2 cut(s) 1308, 1342
HaeIII GGCC 3 cut(s) 1132, 1235, 1449
HapII CCGG 8 cut(s) 169, 273, 304, 543, 788, 972, 1627, 1704
Hin1II CATG 6 cut(s) 229, 806, 1018, 1032, 1478, 1531
HincII GTYRAC 2 cut(s) 128, 1150
HindII GTYRAC 2 cut(s) 128, 1150
HindIII AAGCTT 1 cut(s) 747
HinfI GANTC 8 cut(s) 80, 443, 602, 700, 1245, 1310, 1458, 1611
HpaI GTTAAC 1 cut(s) 1150
HpaII CCGG 8 cut(s) 169, 273, 304, 543, 788, 972, 1627, 1704
HphI GGTGA 2 cut(s) 629, 903
Hpy166II GTNNAC 5 cut(s) 128, 763, 994, 1150, 1472
Hpy188I TCNGA 8 cut(s) 149, 257, 288, 331, 435, 816, 948, 1066
Hpy188III TCNNGA 9 cut(s) 169, 364, 686, 1239, 1280, 1325, 1359, 1523, 1615
Hpy8I GTNNAC 5 cut(s) 128, 763, 994, 1150, 1472
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 6 cut(s) 206, 592, 650, 1007, 1037, 1526
HpyCH4III ACNGT 5 cut(s) 415, 760, 858, 1384, 1486
HpyCH4V TGCA 6 cut(s) 525, 879, 1319, 1478, 1671, 1760
HpyF3I CTNAG 5 cut(s) 146, 930, 1056, 1257, 1394
Hsp92II CATG 6 cut(s) 229, 806, 1018, 1032, 1478, 1531
Kpn2I TCCGGA 1 cut(s) 168
KpnI GGTACC 1 cut(s) 1607
KspAI GTTAAC 1 cut(s) 1150
KspI CCGCGG 1 cut(s) 575
Kzo9I GATC 4 cut(s) 634, 943, 1117, 1620
LguI GCTCTTC 2 cut(s) 1045, 1734
LmnI GCTCC 6 cut(s) 288, 463, 1197, 1276, 1330, 1724
Lsp1109I GCAGC 3 cut(s) 56, 520, 1354
LweI GCATC 4 cut(s) 378, 1179, 1560, 1680
MaeI CTAG 3 cut(s) 462, 926, 1574
MaeIII GTNAC 6 cut(s) 56, 182, 470, 710, 1408, 1653
MalI GATC 4 cut(s) 636, 945, 1119, 1622
MboI GATC 4 cut(s) 634, 943, 1117, 1620
MhlI GDGCHC 2 cut(s) 293, 1729
MluCI AATT 5 cut(s) 334, 368, 406, 886, 1690
MlyI GAGTC 4 cut(s) 89, 694, 1319, 1620
MmeI TCCRAC 4 cut(s) 235, 354, 1073, 1767
Mph1103I ATGCAT 1 cut(s) 1673
MroI TCCGGA 1 cut(s) 168
MroXI GAANNNNTTC 1 cut(s) 1287
MseI TTAA 7 cut(s) 312, 476, 501, 552, 794, 1140, 1149
MslI CAYNNNNRTG 2 cut(s) 1027, 1532
MspA1I CMGCKG 1 cut(s) 574
MspI CCGG 8 cut(s) 169, 273, 304, 543, 788, 972, 1627, 1704
MspR9I CCNGG 2 cut(s) 273, 544
Mva1269I GAATGC 3 cut(s) 1022, 1667, 1673
MvnI CGCG 1 cut(s) 574
NciI CCSGG 2 cut(s) 273, 544
NdeII GATC 4 cut(s) 634, 943, 1117, 1620
NlaIII CATG 6 cut(s) 229, 806, 1018, 1032, 1478, 1531
NlaIV GGNNCC 3 cut(s) 290, 1272, 1605
NmeAIII GCCGAG 1 cut(s) 944
NmuCI GTSAC 1 cut(s) 1408
NsiI ATGCAT 1 cut(s) 1673
NspI RCATGY 1 cut(s) 1478
PciSI GCTCTTC 2 cut(s) 1045, 1734
PcsI WCGNNNNNNNCGW 1 cut(s) 1117
PctI GAATGC 3 cut(s) 1022, 1667, 1673
PdmI GAANNNNTTC 1 cut(s) 1287
PfeI GAWTC 4 cut(s) 443, 602, 1245, 1458
PkrI GCNGC 4 cut(s) 71, 135, 510, 1369
Ple19I CGATCG 1 cut(s) 946
PleI GAGTC 4 cut(s) 88, 694, 1318, 1619
PpsI GAGTC 4 cut(s) 88, 694, 1318, 1619
Psp124BI GAGCTC 1 cut(s) 1729
PspN4I GGNNCC 3 cut(s) 290, 1272, 1605
PspPI GGNCC 4 cut(s) 236, 991, 1131, 1447
PvuI CGATCG 1 cut(s) 946
RsaI GTAC 1 cut(s) 1605
RsaNI GTAC 1 cut(s) 1604
RseI CAYNNNNRTG 2 cut(s) 1027, 1532
SacI GAGCTC 1 cut(s) 1729
SacII CCGCGG 1 cut(s) 575
SapI GCTCTTC 2 cut(s) 1045, 1734
SaqAI TTAA 7 cut(s) 312, 476, 501, 552, 794, 1140, 1149
SatI GCNGC 4 cut(s) 70, 134, 509, 1368
Sau3AI GATC 4 cut(s) 634, 943, 1117, 1620
Sau96I GGNCC 4 cut(s) 236, 991, 1131, 1447
SchI GAGTC 4 cut(s) 89, 694, 1319, 1620
ScrFI CCNGG 2 cut(s) 273, 544
SduI GDGCHC 2 cut(s) 293, 1729
SfaNI GCATC 4 cut(s) 378, 1179, 1560, 1680
SfcI CTRYAG 2 cut(s) 537, 1766
Sfr303I CCGCGG 1 cut(s) 575
SgrBI CCGCGG 1 cut(s) 575
SinI GGWCC 2 cut(s) 236, 991
SmiMI CAYNNNNRTG 2 cut(s) 1027, 1532
SmlI CTYRAG 2 cut(s) 347, 1416
SmoI CTYRAG 2 cut(s) 347, 1416
Sse9I AATT 5 cut(s) 334, 368, 406, 886, 1690
SsiI CCGC 6 cut(s) 109, 133, 342, 495, 572, 574
SspMI CTAG 3 cut(s) 462, 926, 1574
SstI GAGCTC 1 cut(s) 1729
StyD4I CCNGG 2 cut(s) 271, 542
StyI CCWWGG 1 cut(s) 1720
TaaI ACNGT 5 cut(s) 415, 760, 858, 1384, 1486
TaqI TCGA 5 cut(s) 942, 1000, 1223, 1453, 1637
TaqII GACCGA 1 cut(s) 979
TasI AATT 5 cut(s) 334, 368, 406, 886, 1690
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 4 cut(s) 443, 602, 1245, 1458
Tru1I TTAA 7 cut(s) 312, 476, 501, 552, 794, 1140, 1149
Tru9I TTAA 7 cut(s) 312, 476, 501, 552, 794, 1140, 1149
TscAI CASTG 1 cut(s) 1389
TseFI GTSAC 1 cut(s) 1408
TseI GCWGC 3 cut(s) 69, 508, 1367
Tsp45I GTSAC 1 cut(s) 1408
TspDTI ATGAA 5 cut(s) 17, 408, 631, 1577, 1721
TspGWI ACGGA 3 cut(s) 110, 1167, 1184
TspRI CASTG 1 cut(s) 1389
VpaK11BI GGWCC 2 cut(s) 236, 991
XagI CCTNNNNNAGG 1 cut(s) 1520
XapI RAATTY 1 cut(s) 368
XceI RCATGY 1 cut(s) 1478
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XmnI GAANNNNTTC 1 cut(s) 1287
XspI CTAG 3 cut(s) 462, 926, 1574
Zsp2I ATGCAT 1 cut(s) 1673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.