Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0008279

Physical Location & Seq

Genomic Coordinates Forward (+)

13748562 .. 13751610

Gene Structure 3049 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g14500.6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGGAACGAAAGCAAAGGCGCACCACCACCAGTACAATGCTAATGGTGCAGGTGGTGGTGGAGGCAACGGCAGTGAGAGCAGCAGCAGCACCTCACCTAGCCCACCTCCGTCGCCTCGCCGTCACTTCACAGTCCCTCACTGCCGCCGGAAGCCCCGCGCAAAAACCATCTCCGTCGTCTCTGGTTTTCTTCTTCTCCGCCGATTCCTCCGCTTACTGGTGGTGCTTCCCCTGCTCTACGTCTCCGGCCTGCTCATGTGCATGGGCCCGTTCTCCGGTCTAGTGGGCCATACCCCTGTTCCCGGTTCCGTTTACCGCAGTCACGAGATGTTTCAGCTGCTCTGGCCTCACATTCTGGCCGATAATTCGACTCCAATTGAGTTGTCCTCTGTATGGAGATACAGAAGAAAGCTGAAAGAGCAGAAGCCTTGCCCGTCGTCAACTGCTGGACTACAATTAGATTCCCCTGGCCCTAGTGGTTACTTGATTGTGGAGGTTAATGGTGGTCTTAACCAACAACGTTCTGCGATTTGCAATGCTGTGGCACTTGCTGGCCTTTTAAATGCAGTTCTACTTATTCCTCGCTTTGAATTCCATAATGTTTGGAGGGATCCGAGCACTTTTACTGATATATACGATGAAGATCATTTCATAGCCACCCTTGAAGGCCATGTTAAAGTGGTTAAAGAACTACCGGAGATGCTGATGGAGAAATATGATCACAACATCACTACCATACCAACCCTTCATGTCCAATCTTGGGCTCCTGTCCGTTATTACACAGGAGAAGTTTATCCTGTCCTGCAAAAGGAGGGGGTTATTCGCATAGCTCCATTTGCTAATAGACTAGCAATGAATGTCCCACCTCCTATTCAGCATTTGAGATGCCTTGCTAATTATGAAGCATTGAAATTCTCTTCTCCCATATCAAATTTGGCAAATAAACTAGTTGAACGAATGACTGAGATGAGTTCAAGGACCCGTGGAAAGTATATTGCAGTCCACCTCCGCTTTGAGGAGGACATGGTGGCATTCTCATGCTGTTTGTATGATGGAGGAGAGGCTGAAAATGTTGCAATGAATTCAATTAGACAAAAAGGATGGGGAGATAAGTTCAAAAGAAAAGGCCGCGTAACTCTACCTGGTCTCAATAGGATTGATGGAAAGTGCCCACTAACCCCCTTGGAGGTTGGAATGATGCTACGGGGTATGGGGTTTGACAACAGCACTTTAATTTATTTAGCCTCAGGGAAAATCTACTGGGCAGAGAAACATTTAGCCCCTCTGCTAAAGATGTTTCCCTATCTGCATACCAAGAAGTCTCTTGCCACCCCAGATGAGCTTGCTCCTTTTGAGGTATCTTACGATCATCGAGATTGGCAGCCTTGGATTATACAGTATGCTTGCTTAGTGAGATATTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

O-FucT

Protein Analysis

475

Amino Acids

53.22

Weight (kDa)

9.34

Isoelectric Point (pI)

50.23

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008279)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G38065 AT1G38065 AT1G38131
fragaria_vesca	FvH4_2g26360 FvH4_2g26360 FvH4_2g26360 FvH4_2g26360
malus_domestica	MD08G1169500.v1.1 MD15G1356100.v1.1
prunus_persica	Prupe.1G503400_v2.0.a1 Prupe.1G503400_v2.0.a1
pyrus_communis	pycom08g14500 pycom15g31810
rosa_chinensis	RchiOBHm_Chr6g0294941
rosa_multiflora	Rmu_sc0025906.1_g000001 Rmu_sc0025907.1_g000001
rosa_roxburghii	Rroxscaffold_7G00172810
rosa_rugosa	Rorug06G0248500
rosa_samantha	Rh6AG361700 Rh6BG369200 Rh6CG375700 Rh6DG362200
rosa_wichuraiana	Rw6G031550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	41
Acc36I	ACCTGC	1 cut(s)	41
AccII	CGCG	2 cut(s)	159, 1131
AciI	CCGC	7 cut(s)	145, 157, 199, 211, 316, 1009, 1129
AclI	AACGTT	1 cut(s)	520
AclWI	GGATC	2 cut(s)	605, 618
AcoI	YGGCCR	1 cut(s)	357
AcsI	RAATTY	4 cut(s)	590, 911, 931, 1081
AfaI	GTAC	1 cut(s)	35
AfiI	CCNNNNNNNGG	8 cut(s)	217, 275, 302, 393, 760, 808, 1015, 1186
AgsI	TTSAA	7 cut(s)	590, 665, 910, 953, 975, 1086, 1117
AhlI	ACTAGT	1 cut(s)	946
AjnI	CCWGG	2 cut(s)	466, 1141
AluBI	AGCT	4 cut(s)	337, 412, 830, 1342
AluI	AGCT	4 cut(s)	337, 412, 830, 1342
Alw21I	GWGCWC	1 cut(s)	620
Alw26I	GTCTC	4 cut(s)	184, 247, 1151, 1326
AlwI	GGATC	2 cut(s)	605, 618
AoxI	GGCC	9 cut(s)	247, 265, 286, 344, 357, 469, 553, 667, 1126
ApaI	GGGCCC	1 cut(s)	269
ApeKI	GCWGC	5 cut(s)	81, 84, 87, 337, 1381
ApoI	RAATTY	4 cut(s)	590, 911, 931, 1081
AspLEI	GCGC	2 cut(s)	22, 161
AspS9I	GGNCC	5 cut(s)	265, 266, 286, 470, 978
AsuC2I	CCSGG	1 cut(s)	303
AsuHPI	GGTGA	1 cut(s)	87
AvaII	GGWCC	1 cut(s)	978
AxyI	CCTNAGG	1 cut(s)	1246
BaeGI	GKGCMC	2 cut(s)	269, 1172
BamHI	GGATCC	1 cut(s)	610
BanII	GRGCYC	2 cut(s)	269, 766
BauI	CACGAG	1 cut(s)	323
Bbv12I	GWGCWC	1 cut(s)	620
BbvI	GCAGC	5 cut(s)	93, 96, 99, 324, 1393
BccI	CCATC	5 cut(s)	176, 700, 1046, 1095, 1154
BceAI	ACGGC	2 cut(s)	85, 105
BciT130I	CCWGG	2 cut(s)	468, 1143
BclI	TGATCA	1 cut(s)	718
BcnI	CCSGG	1 cut(s)	303
BcoDI	GTCTC	4 cut(s)	184, 247, 1151, 1326
BcuI	ACTAGT	1 cut(s)	946
BfaI	CTAG	5 cut(s)	99, 281, 474, 848, 947
BfuAI	ACCTGC	1 cut(s)	41
BisI	GCNGC	7 cut(s)	82, 85, 88, 145, 338, 1129, 1382
BlsI	GCNGC	7 cut(s)	83, 86, 89, 146, 339, 1130, 1383
Bme1390I	CCNGG	3 cut(s)	303, 468, 1143
Bme18I	GGWCC	1 cut(s)	978
BmgT120I	GGNCC	5 cut(s)	265, 266, 286, 470, 978
BmiI	GGNNCC	5 cut(s)	267, 307, 612, 765, 980
BmrFI	CCNGG	3 cut(s)	303, 468, 1143
BmrI	ACTGGG	1 cut(s)	1270
BmsI	GCATC	3 cut(s)	690, 875, 1188
BmuI	ACTGGG	1 cut(s)	1270
BplI	GAGNNNNNCTC	2 cut(s)	371, 403
BpuMI	CCSGG	1 cut(s)	303
BsaBI	GATNNNNATC	1 cut(s)	642
BsaI	GGTCTC	1 cut(s)	1151
BsaJI	CCNNGG	4 cut(s)	466, 982, 1182, 1385
BsaWI	WCCGGW	2 cut(s)	275, 694
Bsc4I	CCNNNNNNNGG	8 cut(s)	217, 275, 302, 393, 760, 808, 1015, 1186
Bse1I	ACTGG	3 cut(s)	31, 222, 1265
Bse21I	CCTNAGG	1 cut(s)	1246
Bse3DI	GCAATG	3 cut(s)	541, 858, 1083
Bse8I	GATNNNNATC	1 cut(s)	642
BseBI	CCWGG	2 cut(s)	468, 1143
BseDI	CCNNGG	4 cut(s)	466, 982, 1182, 1385
BseGI	GGATG	1 cut(s)	1106
BseJI	GATNNNNATC	1 cut(s)	642
BseLI	CCNNNNNNNGG	8 cut(s)	217, 275, 302, 393, 760, 808, 1015, 1186
BseMI	GCAATG	3 cut(s)	541, 858, 1083
BseMII	CTCAG	2 cut(s)	954, 1260
BseNI	ACTGG	3 cut(s)	31, 222, 1265
BseRI	GAGGAG	2 cut(s)	1031, 1071
BseSI	GKGCMC	2 cut(s)	269, 1172
BseXI	GCAGC	5 cut(s)	93, 96, 99, 324, 1393
BsgI	GTGCAG	1 cut(s)	69
Bsh1236I	CGCG	2 cut(s)	159, 1131
BshFI	GGCC	9 cut(s)	249, 267, 288, 346, 359, 471, 555, 669, 1128
BsiHKAI	GWGCWC	1 cut(s)	620
BsiSI	CCGG	5 cut(s)	148, 246, 276, 303, 695
BslFI	GGGAC	2 cut(s)	119, 845
BslI	CCNNNNNNNGG	8 cut(s)	217, 275, 302, 393, 760, 808, 1015, 1186
BsmAI	GTCTC	4 cut(s)	184, 247, 1151, 1326
BsmBI	CGTCTC	2 cut(s)	184, 247
BsmFI	GGGAC	2 cut(s)	119, 845
BsmI	GAATGC	1 cut(s)	1031
BsnI	GGCC	9 cut(s)	249, 267, 288, 346, 359, 471, 555, 669, 1128
Bso31I	GGTCTC	1 cut(s)	1151
Bsp120I	GGGCCC	1 cut(s)	265
Bsp1286I	GDGCHC	4 cut(s)	269, 620, 766, 1172
Bsp143I	GATC	4 cut(s)	610, 643, 718, 1366
BspACI	CCGC	7 cut(s)	145, 157, 199, 211, 316, 1009, 1129
BspANI	GGCC	9 cut(s)	249, 267, 288, 346, 359, 471, 555, 669, 1128
BspCNI	CTCAG	2 cut(s)	955, 1259
BspFNI	CGCG	2 cut(s)	159, 1131
BspLI	GGNNCC	5 cut(s)	267, 307, 612, 765, 980
BspMI	ACCTGC	1 cut(s)	41
BspPI	GGATC	2 cut(s)	605, 618
BspTNI	GGTCTC	1 cut(s)	1151
BsrDI	GCAATG	3 cut(s)	541, 858, 1083
BsrI	ACTGG	3 cut(s)	31, 222, 1265
BssECI	CCNNGG	4 cut(s)	466, 982, 1182, 1385
BssMI	GATC	4 cut(s)	610, 643, 718, 1366
BssSI	CACGAG	1 cut(s)	323
BssT1I	CCWWGG	2 cut(s)	1182, 1385
Bst2BI	CACGAG	1 cut(s)	323
Bst2UI	CCWGG	2 cut(s)	468, 1143
Bst4CI	ACNGT	2 cut(s)	133, 1398
Bst6I	CTCTTC	1 cut(s)	922
BstC8I	GCNNGC	4 cut(s)	251, 553, 1344, 1405
BstDEI	CTNAG	3 cut(s)	963, 1246, 1408
BstDSI	CCRYGG	1 cut(s)	982
BstENI	CCTNNNNNAGG	1 cut(s)	806
BstF5I	GGATG	1 cut(s)	1106
BstFNI	CGCG	2 cut(s)	159, 1131
BstHHI	GCGC	2 cut(s)	22, 161
BstKTI	GATC	4 cut(s)	613, 646, 721, 1369
BstMAI	GTCTC	4 cut(s)	184, 247, 1151, 1326
BstMBI	GATC	4 cut(s)	610, 643, 718, 1366
BstMWI	GCNNNNNNNGC	7 cut(s)	47, 78, 87, 232, 343, 418, 836
BstNI	CCWGG	2 cut(s)	468, 1143
BstSCI	CCNGG	3 cut(s)	301, 466, 1141
BstSLI	GKGCMC	2 cut(s)	269, 1172
BstUI	CGCG	2 cut(s)	159, 1131
BstV1I	GCAGC	5 cut(s)	93, 96, 99, 324, 1393
BstX2I	RGATCY	1 cut(s)	610
BstYI	RGATCY	1 cut(s)	610
Bsu36I	CCTNAGG	1 cut(s)	1246
BsuRI	GGCC	9 cut(s)	249, 267, 288, 346, 359, 471, 555, 669, 1128
BtgI	CCRYGG	1 cut(s)	982
BtsCI	GGATG	1 cut(s)	1106
BtsI	GCAGTG	2 cut(s)	79, 139
BtsIMutI	CAGTG	2 cut(s)	79, 139
BveI	ACCTGC	1 cut(s)	41
Cac8I	GCNNGC	4 cut(s)	251, 553, 1344, 1405
CfoI	GCGC	2 cut(s)	22, 161
Cfr13I	GGNCC	5 cut(s)	265, 266, 286, 470, 978
CsiI	ACCWGGT	1 cut(s)	1141
Csp6I	GTAC	1 cut(s)	34
CviAII	CATG	6 cut(s)	256, 262, 671, 749, 1024, 1038
CviQI	GTAC	1 cut(s)	34
DdeI	CTNAG	3 cut(s)	963, 1246, 1408
DpnI	GATC	4 cut(s)	612, 645, 720, 1368
DpnII	GATC	4 cut(s)	610, 643, 718, 1366
DraI	TTTAAA	1 cut(s)	561
EaeI	YGGCCR	1 cut(s)	357
Eam1104I	CTCTTC	1 cut(s)	922
EarI	CTCTTC	1 cut(s)	922
EciI	GGCGGA	1 cut(s)	188
Eco130I	CCWWGG	2 cut(s)	1182, 1385
Eco24I	GRGCYC	2 cut(s)	269, 766
Eco31I	GGTCTC	1 cut(s)	1151
Eco47I	GGWCC	1 cut(s)	978
Eco81I	CCTNAGG	1 cut(s)	1246
EcoNI	CCTNNNNNAGG	1 cut(s)	806
EcoO109I	RGGNCCY	1 cut(s)	978
EcoRI	GAATTC	2 cut(s)	590, 1081
EcoRII	CCWGG	2 cut(s)	466, 1141
EcoT14I	CCWWGG	2 cut(s)	1182, 1385
EcoT38I	GRGCYC	2 cut(s)	269, 766
ErhI	CCWWGG	2 cut(s)	1182, 1385
Esp3I	CGTCTC	2 cut(s)	184, 247
FaeI	CATG	6 cut(s)	259, 265, 674, 752, 1027, 1041
FaqI	GGGAC	2 cut(s)	119, 845
FatI	CATG	6 cut(s)	255, 261, 670, 748, 1023, 1037
FauI	CCCGC	1 cut(s)	164
FbaI	TGATCA	1 cut(s)	718
Fnu4HI	GCNGC	7 cut(s)	82, 85, 88, 145, 338, 1129, 1382
FokI	GGATG	1 cut(s)	1113
FriOI	GRGCYC	2 cut(s)	269, 766
Fsp4HI	GCNGC	7 cut(s)	82, 85, 88, 145, 338, 1129, 1382
FspBI	CTAG	5 cut(s)	99, 281, 474, 848, 947
GlaI	GCGC	2 cut(s)	21, 160
GluI	GCNGC	7 cut(s)	82, 85, 88, 145, 338, 1129, 1382
HaeIII	GGCC	9 cut(s)	249, 267, 288, 346, 359, 471, 555, 669, 1128
HapII	CCGG	5 cut(s)	148, 246, 276, 303, 695
HhaI	GCGC	2 cut(s)	22, 161
Hin1II	CATG	6 cut(s)	259, 265, 674, 752, 1027, 1041
Hin6I	GCGC	2 cut(s)	20, 159
HinP1I	GCGC	2 cut(s)	20, 159
HincII	GTYRAC	1 cut(s)	441
HindII	GTYRAC	1 cut(s)	441
HinfI	GANTC	3 cut(s)	204, 370, 461
HpaII	CCGG	5 cut(s)	148, 246, 276, 303, 695
HphI	GGTGA	1 cut(s)	87
Hpy166II	GTNNAC	3 cut(s)	313, 441, 1003
Hpy188I	TCNGA	1 cut(s)	615
Hpy188III	TCNNGA	2 cut(s)	323, 1373
Hpy8I	GTNNAC	3 cut(s)	313, 441, 1003
Hpy99I	CGWCG	3 cut(s)	115, 179, 439
HpyAV	CCTTC	2 cut(s)	659, 755
HpyCH4III	ACNGT	2 cut(s)	133, 1398
HpyCH4IV	ACGT	2 cut(s)	240, 520
HpyCH4V	TGCA	8 cut(s)	50, 261, 534, 566, 805, 998, 1076, 1309
HpyF10VI	GCNNNNNNNGC	7 cut(s)	47, 78, 87, 232, 343, 418, 836
HpyF3I	CTNAG	3 cut(s)	963, 1246, 1408
HpySE526I	ACGT	2 cut(s)	240, 520
Hsp92II	CATG	6 cut(s)	259, 265, 674, 752, 1027, 1041
HspAI	GCGC	2 cut(s)	20, 159
Ksp22I	TGATCA	1 cut(s)	718
Kzo9I	GATC	4 cut(s)	610, 643, 718, 1366
LmnI	GCTCC	3 cut(s)	769, 835, 1351
Lsp1109I	GCAGC	5 cut(s)	93, 96, 99, 324, 1393
LweI	GCATC	3 cut(s)	690, 875, 1188
MabI	ACCWGGT	1 cut(s)	1141
MaeI	CTAG	5 cut(s)	99, 281, 474, 848, 947
MaeII	ACGT	2 cut(s)	240, 520
MaeIII	GTNAC	4 cut(s)	122, 320, 479, 1132
MalI	GATC	4 cut(s)	612, 645, 720, 1368
MboI	GATC	4 cut(s)	610, 643, 718, 1366
MboII	GAAGA	5 cut(s)	182, 185, 417, 653, 909
MfeI	CAATTG	1 cut(s)	375
MflI	RGATCY	1 cut(s)	610
MhlI	GDGCHC	4 cut(s)	269, 620, 766, 1172
MlyI	GAGTC	1 cut(s)	364
MmeI	TCCRAC	1 cut(s)	1171
MseI	TTAA	6 cut(s)	498, 510, 560, 675, 684, 1232
MslI	CAYNNNNRTG	2 cut(s)	260, 1036
MspA1I	CMGCKG	1 cut(s)	337
MspI	CCGG	5 cut(s)	148, 246, 276, 303, 695
MspR9I	CCNGG	3 cut(s)	303, 468, 1143
MunI	CAATTG	1 cut(s)	375
Mva1269I	GAATGC	1 cut(s)	1031
MvaI	CCWGG	2 cut(s)	468, 1143
MvnI	CGCG	2 cut(s)	159, 1131
MwoI	GCNNNNNNNGC	7 cut(s)	47, 78, 87, 232, 343, 418, 836
NciI	CCSGG	1 cut(s)	303
NdeII	GATC	4 cut(s)	610, 643, 718, 1366
NlaIII	CATG	6 cut(s)	259, 265, 674, 752, 1027, 1041
NlaIV	GGNNCC	5 cut(s)	267, 307, 612, 765, 980
NmuCI	GTSAC	2 cut(s)	122, 320
PaqCI	CACCTGC	1 cut(s)	41
PctI	GAATGC	1 cut(s)	1031
PfeI	GAWTC	2 cut(s)	204, 461
PkrI	GCNGC	7 cut(s)	83, 86, 89, 146, 339, 1130, 1383
PleI	GAGTC	1 cut(s)	364
PpsI	GAGTC	1 cut(s)	364
PpuMI	RGGWCCY	1 cut(s)	978
Psp1406I	AACGTT	1 cut(s)	520
Psp5II	RGGWCCY	1 cut(s)	978
Psp6I	CCWGG	2 cut(s)	466, 1141
PspGI	CCWGG	2 cut(s)	466, 1141
PspN4I	GGNNCC	5 cut(s)	267, 307, 612, 765, 980
PspOMI	GGGCCC	1 cut(s)	265
PspPI	GGNCC	5 cut(s)	265, 266, 286, 470, 978
PspPPI	RGGWCCY	1 cut(s)	978
PsuI	RGATCY	1 cut(s)	610
PvuII	CAGCTG	1 cut(s)	337
RsaI	GTAC	1 cut(s)	35
RsaNI	GTAC	1 cut(s)	34
RseI	CAYNNNNRTG	2 cut(s)	260, 1036
SaqAI	TTAA	6 cut(s)	498, 510, 560, 675, 684, 1232
SatI	GCNGC	7 cut(s)	82, 85, 88, 145, 338, 1129, 1382
Sau3AI	GATC	4 cut(s)	610, 643, 718, 1366
Sau96I	GGNCC	5 cut(s)	265, 266, 286, 470, 978
SchI	GAGTC	1 cut(s)	364
ScrFI	CCNGG	3 cut(s)	303, 468, 1143
SduI	GDGCHC	4 cut(s)	269, 620, 766, 1172
SexAI	ACCWGGT	1 cut(s)	1141
SfaNI	GCATC	3 cut(s)	690, 875, 1188
SinI	GGWCC	1 cut(s)	978
SmiMI	CAYNNNNRTG	2 cut(s)	260, 1036
SpeI	ACTAGT	1 cut(s)	946
SsiI	CCGC	7 cut(s)	145, 157, 199, 211, 316, 1009, 1129
SspMI	CTAG	5 cut(s)	99, 281, 474, 848, 947
StyD4I	CCNGG	3 cut(s)	301, 466, 1141
StyI	CCWWGG	2 cut(s)	1182, 1385
TaaI	ACNGT	2 cut(s)	133, 1398
TaiI	ACGT	2 cut(s)	243, 523
TaqI	TCGA	2 cut(s)	368, 1372
TatI	WGTACW	1 cut(s)	33
TauI	GCSGC	2 cut(s)	147, 1131
TfiI	GAWTC	2 cut(s)	204, 461
Tru1I	TTAA	6 cut(s)	498, 510, 560, 675, 684, 1232
Tru9I	TTAA	6 cut(s)	498, 510, 560, 675, 684, 1232
TscAI	CASTG	2 cut(s)	79, 146
TseFI	GTSAC	2 cut(s)	122, 320
TseI	GCWGC	5 cut(s)	81, 84, 87, 337, 1381
Tsp45I	GTSAC	2 cut(s)	122, 320
TspDTI	ATGAA	6 cut(s)	640, 654, 737, 869, 915, 1094
TspGWI	ACGGA	4 cut(s)	99, 163, 298, 761
TspRI	CASTG	2 cut(s)	79, 146
VpaK11BI	GGWCC	1 cut(s)	978
XagI	CCTNNNNNAGG	1 cut(s)	806
XapI	RAATTY	4 cut(s)	590, 911, 931, 1081
XcmI	CCANNNNNNNNNTGG	1 cut(s)	931
XspI	CTAG	5 cut(s)	99, 281, 474, 848, 947

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.