FvH4_2g26360

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008279
Physical Location & Seq
Reverse (-)
21187754 .. 21192614
4861 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26360.t2

Sequence Viewer

Length: 1467 bp
ATGGGAGCCAAAGCCAAGTCCCACCACCATTACAACGGCGGCAATGGCGGTGGCTGCGGCGGCAGCGAGAGCAGCAGCAGCAACTCTCCTAGCCCGCCACCGTCACCGCGTCGTAACTCTAGCTCATTAGTTTTCCACCACCGCCGGAAACTCCGGTCGAAACCCATGTCGGTCGTCGCCGGCTTCCTGGTCCTCCGCCGGTTCCTGCGGCTGCTGGTGGTTCTGCCCGTGCTCTATGTCTCTGGACTTGTCATGTGTGTCGGTGTGGGCCCATTTTGCTCTCTGGTTGGTCATACCCCTCACCCCGGTTCGGTTTACCGGAGCCACGAGATGTTTCAGCTACTCTGGCCTCATATTCAGGCCGACAATTCCACTCCGATCGAGTTGTCATCCGTGTGGAGATACAGAAGGAAGCTGAAAGAACAGAAACCTTGTCCACATTCAACTGCAGGACCACAGTCCGACAATGCTGCCCCTAGTGGTTACTTGATAGTGGAGGTAAATGGCGGTCTTAACCAACAACGCTCTGCGATTTGCAACGCTGTGGCTCTTGCTGGAGTTTTGAATGCAATTCTAGTTATTCCTCGCTTTGAATTCAATAGTGTTTGGAGAGATCAAAGTACTTTTAGCGATATATATGATGAAGAGCATTTCACAGCTACCCTTGAGGGCTATGTTAGAGTGGTTAAAGAACTACCTGAGGCACTGCTGGAGAGATATGATCACAACATCAGTAACATACCAAATATTCGTGTTGAAGCTTGGGCTCCTGTCCGGTATTACACAGGAGAAGTTTATCCTGTTCTGCAGAGCCAGGGGGTTGTAAGGATAGCTCCATTTGCTAATAGGTTATCGATGAGTGTCCCACCTCCTATTCAGCATCTAAGATGCCTTTCTAATTATGAAGCATTGCAATTCTCGTCTTCCATATCAAATTTGGCAAAGAAGCTAGTTGAACGAATGACAGAGAAGAGTTCAAGGAGTGGTGGAAAGTATGTTTCAATCCACCTCCGTTTTGAGGAGGACATGGTGGCATTCTCATGCTGCTTGTATGATGGAGGAGAGGCAGAAAAAGCGGAAATGAATTTAGTACGACAGAGGGGGTGGGGAAATAAGTTCAAAAGTAAAGGCGGTGTAACTCTGCCCGGTTTAAATAGGATTAATGGAAAATGTCCACTCACCCCCTTGGAGGTTGGAATGATGCTAAGAGGTATGGGATTTGATAATACTACTTCAATATATTTAGCCTCGGGGAAAATATATTGGGCAGAAAAGCATTTAGCCCCTCTGCTGAAGATGTTTCCCTATCTGCACACTAAAGAGTCACTTGCAACGCCAGATGAGCTTGCTCCATTTAAGGGATATTCTTCAAGATTGGCAGCTTTGGACTATACAGTATGCTTATTTAGTGAGGTTTTTGTAACAACCCAAGGACATGGGTATCAGTTGGACAGATTTCAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

489

Amino Acids

54.24

Weight (kDa)

9.26

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 162 - 491 7.6e-98 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 109
AcsI RAATTY 3 cut(s) 593, 934, 1084
AcuI CTGAAG 1 cut(s) 1313
AfaI GTAC 2 cut(s) 622, 1092
AfiI CCNNNNNNNGG 5 cut(s) 305, 310, 1018, 1189, 1358
AjnI CCWGG 2 cut(s) 186, 813
AluBI AGCT 9 cut(s) 123, 340, 415, 659, 761, 833, 949, 1345, 1382
AluI AGCT 9 cut(s) 123, 340, 415, 659, 761, 833, 949, 1345, 1382
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 1249
AoxI GGCC 3 cut(s) 268, 347, 360
ApaI GGGCCC 1 cut(s) 272
ApeKI GCWGC 9 cut(s) 54, 63, 72, 75, 78, 211, 470, 1044, 1379
ApoI RAATTY 3 cut(s) 593, 934, 1084
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AseI ATTAAT 1 cut(s) 1161
AspS9I GGNCC 4 cut(s) 190, 268, 269, 452
AsuC2I CCSGG 2 cut(s) 306, 1146
AsuHPI GGTGA 3 cut(s) 96, 293, 1171
AvaI CYCGRG 1 cut(s) 1249
AvaII GGWCC 2 cut(s) 190, 452
AxyI CCTNAGG 1 cut(s) 699
BaeGI GKGCMC 1 cut(s) 272
BaeI ACNNNNGTAYC 2 cut(s) 1424, 1457
BanII GRGCYC 2 cut(s) 272, 769
BauI CACGAG 1 cut(s) 326
BbsI GAAGAC 1 cut(s) 915
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 9 cut(s) 41, 75, 84, 87, 90, 198, 457, 1031, 1391
BccI CCATC 1 cut(s) 1049
BceAI ACGGC 1 cut(s) 52
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 2 cut(s) 188, 815
BclI TGATCA 1 cut(s) 721
BcnI CCSGG 2 cut(s) 306, 1146
BcoDI GTCTC 1 cut(s) 244
BfaI CTAG 5 cut(s) 90, 120, 477, 575, 950
BfmI CTRYAG 2 cut(s) 447, 806
BmcAI AGTACT 1 cut(s) 622
Bme1390I CCNGG 4 cut(s) 188, 306, 815, 1146
Bme18I GGWCC 2 cut(s) 190, 452
BmeT110I CYCGRG 1 cut(s) 1249
BmgT120I GGNCC 4 cut(s) 190, 268, 269, 452
BmiI GGNNCC 5 cut(s) 7, 203, 270, 323, 768
BmrFI CCNGG 4 cut(s) 188, 306, 815, 1146
BmsI GCATC 3 cut(s) 878, 889, 1191
BoxI GACNNNNGTC 1 cut(s) 457
BpiI GAAGAC 1 cut(s) 915
BpmI CTGGAG 2 cut(s) 576, 731
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 2 cut(s) 306, 1146
Bsa29I ATCGAT 1 cut(s) 854
BsaJI CCNNGG 5 cut(s) 304, 814, 1185, 1248, 1429
BsaWI WCCGGW 3 cut(s) 153, 318, 774
Bsc4I CCNNNNNNNGG 5 cut(s) 305, 310, 1018, 1189, 1358
Bse118I RCCGGY 2 cut(s) 179, 198
Bse21I CCTNAGG 1 cut(s) 699
Bse3DI GCAATG 2 cut(s) 49, 908
BseBI CCWGG 2 cut(s) 188, 815
BseCI ATCGAT 1 cut(s) 854
BseDI CCNNGG 5 cut(s) 304, 814, 1185, 1248, 1429
BseGI GGATG 1 cut(s) 389
BseLI CCNNNNNNNGG 5 cut(s) 305, 310, 1018, 1189, 1358
BseMI GCAATG 2 cut(s) 49, 908
BseMII CTCAG 1 cut(s) 690
BseRI GAGGAG 2 cut(s) 1034, 1074
BseSI GKGCMC 1 cut(s) 272
BseXI GCAGC 9 cut(s) 41, 75, 84, 87, 90, 198, 457, 1031, 1391
BsgI GTGCAG 1 cut(s) 1295
Bsh1236I CGCG 1 cut(s) 109
Bsh1285I CGRYCG 3 cut(s) 158, 174, 381
BshFI GGCC 3 cut(s) 270, 349, 362
BshVI ATCGAT 1 cut(s) 854
BsiEI CGRYCG 3 cut(s) 158, 174, 381
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 1249
BsiSI CCGG 8 cut(s) 145, 154, 180, 199, 306, 319, 775, 1146
BslFI GGGAC 2 cut(s) 4, 848
BslI CCNNNNNNNGG 5 cut(s) 305, 310, 1018, 1189, 1358
BsmAI GTCTC 1 cut(s) 244
BsmFI GGGAC 2 cut(s) 4, 848
BsmI GAATGC 2 cut(s) 571, 1034
BsnI GGCC 3 cut(s) 270, 349, 362
BsoBI CYCGRG 1 cut(s) 1249
Bsp120I GGGCCC 1 cut(s) 268
Bsp1286I GDGCHC 3 cut(s) 234, 272, 769
Bsp143I GATC 3 cut(s) 378, 613, 721
BspANI GGCC 3 cut(s) 270, 349, 362
BspCNI CTCAG 1 cut(s) 691
BspDI ATCGAT 1 cut(s) 854
BspFNI CGCG 1 cut(s) 109
BspLI GGNNCC 5 cut(s) 7, 203, 270, 323, 768
BspMAI CTGCAG 2 cut(s) 451, 810
BspQI GCTCTTC 1 cut(s) 639
BsrDI GCAATG 2 cut(s) 49, 908
BsrFI RCCGGY 2 cut(s) 179, 198
BssAI RCCGGY 2 cut(s) 179, 198
BssECI CCNNGG 5 cut(s) 304, 814, 1185, 1248, 1429
BssMI GATC 3 cut(s) 378, 613, 721
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 2 cut(s) 1185, 1429
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 2 cut(s) 188, 815
Bst4CI ACNGT 3 cut(s) 102, 459, 1396
Bst6I CTCTTC 2 cut(s) 639, 965
BstC8I GCNNGC 3 cut(s) 95, 181, 1347
BstDEI CTNAG 3 cut(s) 699, 884, 1205
BstF5I GGATG 1 cut(s) 389
BstFNI CGCG 1 cut(s) 109
BstKTI GATC 3 cut(s) 381, 616, 724
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 3 cut(s) 378, 613, 721
BstMCI CGRYCG 3 cut(s) 158, 174, 381
BstNI CCWGG 2 cut(s) 188, 815
BstPAI GACNNNNGTC 1 cut(s) 457
BstSCI CCNGG 4 cut(s) 186, 304, 813, 1144
BstSFI CTRYAG 2 cut(s) 447, 806
BstSLI GKGCMC 1 cut(s) 272
BstUI CGCG 1 cut(s) 109
BstV1I GCAGC 9 cut(s) 41, 75, 84, 87, 90, 198, 457, 1031, 1391
BstV2I GAAGAC 1 cut(s) 915
BstXI CCANNNNNNTGG 1 cut(s) 1436
Bsu15I ATCGAT 1 cut(s) 854
Bsu36I CCTNAGG 1 cut(s) 699
BsuRI GGCC 3 cut(s) 270, 349, 362
BsuTUI ATCGAT 1 cut(s) 854
BtsCI GGATG 1 cut(s) 389
BtsI GCAGTG 1 cut(s) 704
BtsIMutI CAGTG 1 cut(s) 704
Cac8I GCNNGC 3 cut(s) 95, 181, 1347
Cfr10I RCCGGY 2 cut(s) 179, 198
Cfr13I GGNCC 4 cut(s) 190, 268, 269, 452
ClaI ATCGAT 1 cut(s) 854
CseI GACGC 1 cut(s) 98
Csp6I GTAC 2 cut(s) 621, 1091
CspCI CAANNNNNGTGG 2 cut(s) 31, 66
CviAII CATG 5 cut(s) 166, 253, 1027, 1041, 1436
CviQI GTAC 2 cut(s) 621, 1091
DdeI CTNAG 3 cut(s) 699, 884, 1205
DpnI GATC 3 cut(s) 380, 615, 723
DpnII GATC 3 cut(s) 378, 613, 721
DraI TTTAAA 1 cut(s) 1152
Eam1104I CTCTTC 2 cut(s) 639, 965
EarI CTCTTC 2 cut(s) 639, 965
EciI GGCGGA 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 1185, 1429
Eco24I GRGCYC 2 cut(s) 272, 769
Eco47I GGWCC 2 cut(s) 190, 452
Eco57I CTGAAG 1 cut(s) 1313
Eco81I CCTNAGG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 1249
EcoRI GAATTC 1 cut(s) 593
EcoRII CCWGG 2 cut(s) 186, 813
EcoT14I CCWWGG 2 cut(s) 1185, 1429
EcoT38I GRGCYC 2 cut(s) 272, 769
ErhI CCWWGG 2 cut(s) 1185, 1429
FaeI CATG 5 cut(s) 169, 256, 1030, 1044, 1439
FalI AAGNNNNNCTT 2 cut(s) 1311, 1343
FaqI GGGAC 2 cut(s) 4, 848
FatI CATG 5 cut(s) 165, 252, 1026, 1040, 1435
FauI CCCGC 1 cut(s) 102
FbaI TGATCA 1 cut(s) 721
FokI GGATG 1 cut(s) 376
FriOI GRGCYC 2 cut(s) 272, 769
FspBI CTAG 5 cut(s) 90, 120, 477, 575, 950
GsuI CTGGAG 2 cut(s) 576, 731
HaeIII GGCC 3 cut(s) 270, 349, 362
HapII CCGG 8 cut(s) 145, 154, 180, 199, 306, 319, 775, 1146
HgaI GACGC 1 cut(s) 98
Hin1II CATG 5 cut(s) 169, 256, 1030, 1044, 1439
HindIII AAGCTT 1 cut(s) 759
HinfI GANTC 1 cut(s) 1322
HpaII CCGG 8 cut(s) 145, 154, 180, 199, 306, 319, 775, 1146
HphI GGTGA 3 cut(s) 96, 293, 1171
Hpy166II GTNNAC 3 cut(s) 316, 437, 1175
Hpy188I TCNGA 3 cut(s) 378, 463, 1461
Hpy188III TCNNGA 2 cut(s) 243, 1371
Hpy8I GTNNAC 3 cut(s) 316, 437, 1175
Hpy99I CGWCG 2 cut(s) 114, 179
HpyAV CCTTC 1 cut(s) 402
HpyCH4III ACNGT 3 cut(s) 102, 459, 1396
HpyCH4V TGCA 7 cut(s) 449, 537, 569, 808, 913, 1312, 1331
HpyF3I CTNAG 3 cut(s) 699, 884, 1205
Hsp92II CATG 5 cut(s) 169, 256, 1030, 1044, 1439
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 721
Kzo9I GATC 3 cut(s) 378, 613, 721
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 5 cut(s) 5, 321, 772, 838, 1354
Lsp1109I GCAGC 9 cut(s) 41, 75, 84, 87, 90, 198, 457, 1031, 1391
LweI GCATC 3 cut(s) 878, 889, 1191
MaeI CTAG 5 cut(s) 90, 120, 477, 575, 950
MaeIII GTNAC 7 cut(s) 102, 113, 482, 734, 1135, 1323, 1420
MalI GATC 3 cut(s) 380, 615, 723
MboI GATC 3 cut(s) 378, 613, 721
MboII GAAGA 5 cut(s) 656, 915, 982, 1306, 1359
MhlI GDGCHC 3 cut(s) 234, 272, 769
MluCI AATT 7 cut(s) 367, 570, 593, 898, 914, 934, 1084
MlyI GAGTC 1 cut(s) 1331
MmeI TCCRAC 3 cut(s) 486, 1174, 1428
MroNI GCCGGC 1 cut(s) 179
MseI TTAA 5 cut(s) 513, 687, 1151, 1161, 1356
MslI CAYNNNNRTG 2 cut(s) 394, 1039
MspI CCGG 8 cut(s) 145, 154, 180, 199, 306, 319, 775, 1146
MspR9I CCNGG 4 cut(s) 188, 306, 815, 1146
Mva1269I GAATGC 2 cut(s) 571, 1034
MvaI CCWGG 2 cut(s) 188, 815
MvnI CGCG 1 cut(s) 109
NaeI GCCGGC 1 cut(s) 181
NciI CCSGG 2 cut(s) 306, 1146
NdeII GATC 3 cut(s) 378, 613, 721
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 5 cut(s) 169, 256, 1030, 1044, 1439
NlaIV GGNNCC 5 cut(s) 7, 203, 270, 323, 768
NmuCI GTSAC 2 cut(s) 102, 1323
PciSI GCTCTTC 1 cut(s) 639
PctI GAATGC 2 cut(s) 571, 1034
PdiI GCCGGC 1 cut(s) 181
Ple19I CGATCG 1 cut(s) 381
PleI GAGTC 1 cut(s) 1330
PpsI GAGTC 1 cut(s) 1330
PshAI GACNNNNGTC 1 cut(s) 457
PshBI ATTAAT 1 cut(s) 1161
Psp6I CCWGG 2 cut(s) 186, 813
PspGI CCWGG 2 cut(s) 186, 813
PspN4I GGNNCC 5 cut(s) 7, 203, 270, 323, 768
PspOMI GGGCCC 1 cut(s) 268
PspPI GGNCC 4 cut(s) 190, 268, 269, 452
PstI CTGCAG 2 cut(s) 451, 810
PvuI CGATCG 1 cut(s) 381
RsaI GTAC 2 cut(s) 622, 1092
RsaNI GTAC 2 cut(s) 621, 1091
RseI CAYNNNNRTG 2 cut(s) 394, 1039
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 5 cut(s) 513, 687, 1151, 1161, 1356
Sau3AI GATC 3 cut(s) 378, 613, 721
Sau96I GGNCC 4 cut(s) 190, 268, 269, 452
ScaI AGTACT 1 cut(s) 622
SchI GAGTC 1 cut(s) 1331
ScrFI CCNGG 4 cut(s) 188, 306, 815, 1146
SduI GDGCHC 3 cut(s) 234, 272, 769
SfaNI GCATC 3 cut(s) 878, 889, 1191
SfcI CTRYAG 2 cut(s) 447, 806
SinI GGWCC 2 cut(s) 190, 452
SmiMI CAYNNNNRTG 2 cut(s) 394, 1039
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
Sse9I AATT 7 cut(s) 367, 570, 593, 898, 914, 934, 1084
SspI AATATT 1 cut(s) 748
SspMI CTAG 5 cut(s) 90, 120, 477, 575, 950
StyD4I CCNGG 4 cut(s) 186, 304, 813, 1144
StyI CCWWGG 2 cut(s) 1185, 1429
TaaI ACNGT 3 cut(s) 102, 459, 1396
TaqI TCGA 3 cut(s) 158, 381, 854
TaqII GACCGA 1 cut(s) 160
TasI AATT 7 cut(s) 367, 570, 593, 898, 914, 934, 1084
TatI WGTACW 1 cut(s) 620
TauI GCSGC 4 cut(s) 42, 60, 63, 211
Tru1I TTAA 5 cut(s) 513, 687, 1151, 1161, 1356
Tru9I TTAA 5 cut(s) 513, 687, 1151, 1161, 1356
TscAI CASTG 1 cut(s) 711
TseFI GTSAC 2 cut(s) 102, 1323
TseI GCWGC 9 cut(s) 54, 63, 72, 75, 78, 211, 470, 1044, 1379
Tsp45I GTSAC 2 cut(s) 102, 1323
TspDTI ATGAA 3 cut(s) 657, 918, 1097
TspGWI ACGGA 2 cut(s) 382, 1001
TspRI CASTG 1 cut(s) 711
VpaK11BI GGWCC 2 cut(s) 190, 452
VspI ATTAAT 1 cut(s) 1161
XapI RAATTY 3 cut(s) 593, 934, 1084
XcmI CCANNNNNNNNNTGG 1 cut(s) 934
XspI CTAG 5 cut(s) 90, 120, 477, 575, 950
ZrmI AGTACT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.